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Id: lil-623383
Autor: Peresi, Eliana; Silva, Sônia Maria Usó Ruiz; Calvi, Sueli Aparecida; Marcondes-Machado, Jussara.
Título: Citocinas e proteínas de fase aguda do soro como marcadores de regressão da resposta inflamatória ao tratamento da tuberculose pulmonar / Cytokines and acute phase serum proteins as markers of inflammatory regression during the treatment of pulmonary tuberculosis
Fonte: J. bras. pneumol;34(11):942-949, nov. 2008. ilus, tab.
Idioma: pt.
Resumo: OBJETIVO: Analisar o padrão de citocinas pró- e antiinflamatórias e da resposta de fase aguda (RFA) como marcadores de resposta ao tratamento da tuberculose pulmonar. MÉTODOS: Determinação dos níveis de interferon-gama (IFN-γ), tumor necrosis factor-alpha (TNF-α, fator de necrose tumoral-alfa), interleucina-10 (IL-10) e transforming growth factor-beta (TGF-β, fator transformador de crescimento-beta), pelo método ELISA, em sobrenadante de cultura de células mononucleares do sangue periférico e monócitos, assim como dos níveis de proteínas totais, albumina, globulinas, alfa-1-glicoproteína ácida (AGA), proteína C reativa (PCR) e velocidade de hemossedimentação (VHS) em 28 doentes com tuberculose pulmonar, em três tempos: antes (T0), aos três meses (T3) e aos seis meses (T6) de tratamento, em relação aos controles saudáveis, em um único tempo. RESULTADOS: Os pacientes apresentaram valores maiores de citocinas e RFA que os controles em T0, com diminuição em T3 e diminuição (TNF-α, IL-10, TGF-β, AGA e VHS) ou normalização (IFN-γ e PCR) em T6. CONCLUSÕES: PCR, AGA e VHS são possíveis marcadores para auxiliar no diagnóstico de tuberculose pulmonar e na indicação de tratamento de indivíduos com baciloscopia negativa; PCR (T0 > T3 > T6 = referência) pode também ser marcador de resposta ao tratamento. Antes do tratamento, o perfil Th0 (IFN-γ, IL-10, TNF-α e TGF-β), indutor de e protetor contra inflamação, prevaleceu nos pacientes; em T6, prevaleceu o perfil Th2 (IL-10, TNF-α e TGF-β), protetor contra efeito nocivo pró-inflamatório do TNF-α ainda presente. O comportamento do IFN-γ (T0 > T3 > T6 = controle) sugere sua utilização como marcador de resposta ao tratamento.

OBJECTIVE: To evaluate the pattern of pro-inflammatory cytokines, anti-inflammatory cytokines and the acute phase response (APR) as markers of the response to treatment of pulmonary tuberculosis. METHODS: Twenty-eight patients with pulmonary tuberculosis were evaluated at three time points: pretreatment (T0), treatment month 3 (T3) and treatment month 6 (T6). Levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukine-10 (IL-10) and transforming growth factor-beta (TGF-β) were determined using ELISA in the supernatant of peripheral blood mononuclear cell and monocyte culture. Levels of total protein, albumin, globulins, C-reactive protein (CRP), alpha-1-acid glycoprotein (AAG) and erythrocyte sedimentation rate (ESR) were also determined. All of these parameters were also evaluated, only once, in a group of healthy controls. RESULTS: In relation to controls, patients presented cytokine levels and APR that were higher at T0, lower at T3 and either lower (TNF-α, IL-10, TGF-β, AAG and ESR) or normal (IFN-γ and CRP) at T6. CONCLUSIONS: For individuals with negative smear sputum microscopy, CRP, AAG and ESR are potential markers of pulmonary tuberculosis and of the need for treatment; CRP (T0 > T3 > T6 = reference) can also be a marker of treatment response. In the patients, the Th0 profile (IFN-γ, IL-10, TNF-α and TGF-β), inducer of and protector against inflammation, predominated at T0, whereas the Th2 profile (IL-10, TNF-α and TGF-β), protecting against the harmful pro-inflammatory effect of the remaining TNF-α, predominated at T6. The behavior of IFN-γ (T0 > T3 > T6 = controls) suggests its use as a marker of treatment response.
Descritores: Proteína C-Reativa/análise
Interferon gama/sangue
/sangue
INTERLEUKIN-ABDUCENS NERVE/sangue
Fator de Crescimento Transformador beta/sangue
Tuberculose Pulmonar/sangue
Fator de Necrose Tumoral alfa/sangue
-Biomarcadores/sangue
Sedimentação Sanguínea/efeitos dos fármacos
Técnicas de Cultura de Células
Ensaio de Imunoadsorção Enzimática
Fatores de Tempo
Tuberculose Pulmonar/tratamento farmacológico
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-1054688
Autor: Santiago, Luiz Alfredo; Silva, Jussara Matyelle Rodrigues da; Azevedo, Orleâncio Gomes Ripardo de; Vasconcelos, Paulo Roberto Leitão de.
Título: Comparative study on the efficacy of non-steroidal, steroid and non-use of anti-inflammatory in the treatment of acute epidemic conjunctivitis
Fonte: Acta cir. bras;34(12):e201901206, 2019. tab.
Idioma: en.
Resumo: Abstract Purpose To evaluate the effects of prednisolone against sodium diclofenac both with ciprofloxacin compared to artificial tears on the symptoms and signs of acute viral conjunctivitis. Methods Study included 37 patients diagnosed with acute conjunctivitis and distributed by three groups: A (1% prednisolone acetate + ciprofloxacin (0.3%); B (Sodium diclofenac (0.1%) + ciprofloxacin (0.3%) and C (artificial tears + ciprofloxacin (0.3%). Patients received medication 6/6 hours daily. Signs and symptoms (e.g. lacrimation, burning, photophobia, etc.) were scored at baseline and on the first, third, fifth and seventh days and in the end of treatment using a standardized questionnaire and slit lamp anterior segment examination. Results All three groups demonstrated an improvement in the signs and symptoms of conjunctivitis in their follow-up visits. There was no significant difference in symptom and sign scores between Group A and B and B and C in the study visits ( p >0.05). However, the comparison between groups A and C showed a clinical trend (p=0.05) on third evaluation suggesting better clinical action using the corticosteroids. Conclusion The prednisolone acetate was not superior to the use of sodium diclofenac or artificial tears in relieving the signs and symptoms of viral conjunctivitis.
Descritores: Prednisolona/análogos & derivados
Ciprofloxacino/administração & dosagem
Anti-Inflamatórios não Esteroides/administração & dosagem
Conjuntivite Viral/tratamento farmacológico
Diclofenaco/administração & dosagem
Corticosteroides/administração & dosagem
-Soluções Oftálmicas/administração & dosagem
Prednisolona/administração & dosagem
Doença Aguda
Análise de Variância
Interleucinas/análise
Interferon gama
Fator de Necrose Tumoral alfa/análise
Resultado do Tratamento
Óxido Nítrico Sintase/análise
Lubrificantes Oftálmicos/administração & dosagem
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-973620
Autor: Silvestre, Marilene Chaves; Reis, Vitor Manoel Silva dos.
Título: Evaluation of the profile of inflammatory cytokines, through immunohistochemistry, in the skin of patients with allergic contact dermatitis to nickel in the acute and chronic phases
Fonte: An. bras. dermatol;93(6):829-835, Nov.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: Abstract: Background: Allergic contact dermatitis to ion nickel (Ni+2) is an inflammatory dermatosis, common in industrialized countries. It involves the activation of nickel-specific T-cells, followed by proliferation and induction of a mixed profile of both proinflammatory and regulatory cytokines, suggesting that several T-cell subtypes (helper - Th and cytotoxic - Tc) are involved. A broader understanding of the cytokine profile may lead to new therapeutic approaches. Objectives: This study aimed to analyze the cytokines TNF-α, INF-γ, IL-2, IL-4, IL-10, IL-13, IL-17 and IL-23 using the immunohistochemistry technique in order to try to identify their prevalence in chronic and acute eczema of patients with allergic contact dermatitis to Ni+2. Methods: We performed an immunohistochemical study for eight cytokines in 20 patients with Ni+2 allergic contact dermatitis, biopsied at the site of chronic eczema, triggered by the patient's daily contact with Ni+2, and at the site of acute eczema caused by nickel sulfate, 48 hours after applying the contact test. Results: The stained samples showed positive results for the eight cytokines studied. TNF-α, IFN-γ, IL-4, IL-13 and IL-17 had a higher prevalence in chronic eczema, IL-2 and IL-23 in acute eczema, and IL-10 presented a similar prevalence in both acute and chronic eczema. However, these prevalences were statistically significant only for IL-4 and IL-13. Study Limitations: Small sample size. Conclusions: In chronic and acute eczema, we observed the presence of a mixed cytokine profile of the T cell subtypes (Th/Tc), suggesting that the responses are expressed at the same time.
Descritores: Citocinas/análise
Interleucinas/análise
Interferon gama/análise
Fator de Necrose Tumoral alfa/análise
Dermatite Alérgica de Contato/imunologia
Níquel/efeitos adversos
-Biópsia
Imuno-Histoquímica
Doença Aguda
Doença Crônica
Estudos Prospectivos
Citocinas/imunologia
Interleucinas/imunologia
Interferon gama/imunologia
Fator de Necrose Tumoral alfa/imunologia
Dermatite Alérgica de Contato/etiologia
Dermatite Alérgica de Contato/patologia
Níquel/imunologia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Tipo de Publ: Estudo Observacional
Responsável: BR1.1 - BIREME


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Id: biblio-837916
Autor: Wang, Fang; Liu, Juan-Hua; Zhao, Yu-Kun; Luo, Di-Qing.
Título: Interferon-gamma-induced local leukocytoclastic vasculitis at the subcutaneous injection site
Fonte: An. bras. dermatol;91(5,supl.1):76-78, Sept.-Oct. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Cutaneous reactions associated with interferons (IFNs) treatment are either localized or generalized. The most common presentation of localized reactions at IFNs injection site is usually an erythematous patch or plaque. Local leukocytoclastic vasculitis presenting with cutaneous necrosis is extremely rare. We report a 19-year-old man with hepatitis B who had local leukocytoclastic vasculitis induced by interferon-gama injection at the injection site. After changing the injection sites and using the combined treatment of prednisone and colchicine, the previous lesion healed and no other cutaneous lesion occurred. We also made a mini review of such cases.
Descritores: Pele/patologia
Interferon gama/efeitos adversos
Vasculite Leucocitoclástica Cutânea/induzido quimicamente
-Pele/efeitos dos fármacos
Prednisona/uso terapêutico
Colchicina/uso terapêutico
Resultado do Tratamento
Vasculite Leucocitoclástica Cutânea/patologia
Vasculite Leucocitoclástica Cutânea/tratamento farmacológico
Eritema/induzido quimicamente
Eritema/patologia
Injeções Subcutâneas/efeitos adversos
Anti-Inflamatórios/uso terapêutico
Necrose/induzido quimicamente
Necrose/patologia
Limites: Humanos
Masculino
Adulto Jovem
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-1048819
Autor: Nejat, Sahar.
Título: Interferon-gamma release assays for tuberculosis screening of children / Ensayos de liberación de interferón gamma para el rastreo de la tuberculosis en niños
Fonte: Salud(i)ciencia (Impresa) = Salud(i)ciencia (En linea);22(7):666-667, oct.-nov. 2017.
Idioma: en; es.
Descritores: Tuberculose
Tuberculose/diagnóstico
Interferon gama
Limites: Pré-Escolar
Criança
Adolescente
Tipo de Publ: Relatório Técnico
Responsável: AR392.1 - Biblioteca


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Id: lil-623608
Autor: Badaro, Roberto.
Título: The use of recombinant gamma interferon associated with pentavalent antimony in therapy for visceral leishmaniasis
Fonte: Mem. Inst. Oswaldo Cruz;83(supl.1):376-377, Nov. 1988.
Idioma: en.
Conferência: Apresentado em: Annual Meeting on Basic Research in Chagas's disease, 15, Apresentado em: Meeting of the Brazilian Society of Protozoology4, Caxambu, 7-10 Nov. 1988.
Descritores: Interferon gama/uso terapêutico
Leishmaniose Visceral/tratamento farmacológico
Antimônio/uso terapêutico
-Proteínas Recombinantes
Leishmaniose Visceral/terapia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-792599
Autor: KAMPITS, Cassio; MONTENEGRO, Marlon M; RIBEIRO, Ingrid WJ; FURTADO, Mariana V; POLANCZYK, Carisi A; RÖSING, Cassiano K; HAAS, Alex. N.
Título: Periodontal disease and inflammatory blood cytokines in patients with stable coronary artery disease
Fonte: J. appl. oral sci;24(4):352-358, July-Aug. 2016. tab.
Idioma: en.
Projeto: National Council for Scientific and Technological Development.
Resumo: ABSTRACT Periodontal disease has been associated with elevations of blood cytokines involved in atherosclerosis in systemically healthy individuals, but little is known about this association in stable cardiovascular patients. The aim of this study was to assess the association between periodontal disease (exposure) and blood cytokine levels (outcomes) in a target population of patients with stable coronary artery disease (CAD). Material and Methods This cross-sectional study included 91 patients with stable CAD who had been under optimized cardiovascular care. Blood levels of IL-1β, IL-6, IL-8, IL-10, IFN-γ, and TNF-α were measured by Luminex technology. A full-mouth periodontal examination was conducted to record probing depth (PD) and clinical attachment (CA) loss. Multiple linear regression models, adjusting for gender, body mass index, oral hypoglycemic drugs, smoking, and occurre:nce of acute myocardial infarction were applied. Results CAD patients that experienced major events had higher concentrations of IFN-γ (median: 5.05 pg/mL vs. 3.01 pg/mL; p=0.01), IL-10 (median: 2.33 pg/mL vs. 1.01 pg/mL; p=0.03), and TNF-α (median: 9.17 pg/mL vs. 7.47 pg/mL; p=0.02). Higher numbers of teeth with at least 6 mm of CA loss (R2=0.07) and PD (R2=0.06) were significantly associated with higher IFN-γ log concentrations. Mean CA loss (R2=0.05) and PD (R2=0.06) were significantly related to IL-10 concentrations. Elevated concentrations of TNF-α were associated with higher mean CA loss (R2=0.07). Conclusion Periodontal disease is associated with increased systemic inflammation in stable cardiovascular patients. These findings provide additional evidence supporting the idea that periodontal disease can be a prognostic factor in cardiovascular patients.
Descritores: Doenças Periodontais/sangue
Doença da Artéria Coronariana/sangue
Interleucinas/sangue
Interferon gama/sangue
Fator de Necrose Tumoral alfa/sangue
-Doenças Periodontais/fisiopatologia
Valores de Referência
Doença da Artéria Coronariana/fisiopatologia
Biomarcadores/sangue
Fumar/efeitos adversos
Modelos Lineares
Índice Periodontal
Estudos Transversais
Valor Preditivo dos Testes
Inquéritos e Questionários
Fatores de Risco
Perda da Inserção Periodontal
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Idoso
Responsável: BR1.1 - BIREME


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Id: lil-777353
Autor: Mendi, Ayşegül; Köse, Sevil; Uçkan, Duygu; Akca, Gülçin; Yilmaz, Derviş; Aral, Levent; Gültekin, Sibel Elif; Eroğlu, Tamer; Kiliç, Emine; Uçkan, Sina.
Título: Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation
Fonte: J. appl. oral sci;24(1):67-75, Jan.-Feb. 2016. tab, graf.
Idioma: en.
Projeto: TUBITAK; . TUBITAK; . TUBITAK.
Resumo: ABSTRACT An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a recognized feature that inhibits CXCL8 expression. Objective The aim of the study was to determine if P. gingivalis infection modulates the inflammatory response of gingival stromal stem cells (G-MSSCs), including the release of CXCL8, and the expression of TLRs and if immunomodulatory L. rhamnosus ATCC9595 could prevent CXCL8 inhibition in experimental inflammation. Material and Methods G-MSSCs were pretreated with L. rhamnosus ATCC9595 and then stimulated with P. gingivalis ATCC33277. CXCL8 and IL-10 levels were investigated with ELISA and the TLR-4 and 2 were determined through flow cytometer analysis. Results CXCL8 was suppressed by P. gingivalis and L. rhamnosus ATCC9595, whereas incubation with both strains did not abolish CXCL8. L. rhamnosus ATCC9595 scaled down the expression of TLR4 and induced TLR2 expression when exposed to P. gingivalis stimulation (p<0.01). Conclusions These findings provide evidence that L. rhamnosus ATCC9595 can modulate the inflammatory signals and could introduce P. gingivalis to immune systems by inducing CXCL8 secretion.
Descritores: Interleucina-8/análise
Porphyromonas gingivalis/imunologia
Probióticos/farmacologia
Lactobacillus rhamnosus/fisiologia
Células-Tronco Mesenquimais/microbiologia
-Periodontite/microbiologia
Aderência Bacteriana/imunologia
Ensaio de Imunoadsorção Enzimática
Células Cultivadas
Interleucina-8/imunologia
Interferon gama/análise
Interferon gama/imunologia
Interleucina-10
Estatísticas não Paramétricas
Receptor 4 Toll-Like/análise
Receptor 4 Toll-Like/imunologia
Citometria de Fluxo
Imunidade Inata
Limites: Humanos
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-894934
Autor: Alvarado-Arnez, Lucia Elena; Batista, Angelica Martins; Alves, Silvia Marinho; Melo, Gloria; Lorena, Virgínia Maria Barros de; Cardoso, Cynthia C; Pereira, Isabela Resende; Carrazzone, Cristina; Pacheco, Antonio G; Oliveira Jr, Wilson; Moraes, Milton Ozório; Lannes-Vieira, Joseli.
Título: Single nucleotide polymorphisms of cytokine-related genes and association with clinical outcome in a Chagas disease case-control study from Brazil
Fonte: Mem. Inst. Oswaldo Cruz;113(6):e170489, 2018. tab, graf.
Idioma: en.
Projeto: FAPERJ; . CNPq; . INCTV, National Institute for Science and Technology for Vaccines; . BPP; . PROEP/IOC/CNPq.
Resumo: BACKGROUND The severity of chronic chagasic cardiomyopathy (CCC), the most frequent clinical outcome of Chagas disease (CD), has been associated with cytokine-enriched heart tissue inflammation, and high serum levels of transforming growth factor (TGFβ), interferon-gamma (IFNγ), and tumour necrosis factor (TNF). Conversely, increased interleukin (IL)-10 serum concentrations have been associated with asymptomatic CD. Cytokines and cytokine-related gene polymorphisms may control cytokine expression and have been proposed to contribute to CCC outcomes. OBJECTIVES We evaluated the association of 13 cytokine-related genes (TGFB: rs8179181, rs8105161, rs1800469; IL10: rs1800890, rs1800871, rs1800896; IFNG: rs2430561; TNF: rs1800629; BAT1: rs3853601; LTA: rs909253, rs2239704; TNFR1: rs767455; TNFR2: rs1061624) with risk and progression of CCC. FINDINGS Four hundred and six seropositive patients from CD endemic areas in the state of Pernambuco, north-eastern Brazil, were classified as non-cardiopathic (A, 110) or cardiopathic (mild, B1, 163; severe, C, 133). We found no evidence of TGFB, IL10, TNF, or TNFR1/2 gene polymorphisms associated with CCC risk or progression. Only BAT1 rs3853601 −22G carriers (B1 vs. C: OR = 0.5; p-value = 0.03) and IFNG rs2430561 +874AT (A vs. C: OR = 0.7; p-value = 0.03; A vs. B1+C: OR = 0.8; p-value = 0.02) showed a significant association with protection from cardiopathy in a logistic regression analysis with adjustment for gender and ethnicity; however, the association disappeared after performing adjustment for multiple testing. A systematic review of TNF rs1800629 −308G>A publications included five studies for meta-analysis (534 CCC and 472 asymptomatic patients) and showed no consensus in pooled odds ratio (OR) estimates for A allele or A carriers (OR = 1.4 and 1.5; p-values = 0.14 and 0.15, respectively). In CD patients, TNF serum levels were increased, but not affected by the TNF rs1800629 −308A allele. MAIN CONCLUSIONS Our data suggest no significant contribution of the analysed gene variants of cytokine-related molecules to development/severity of Chagas' heart disease, reinforcing the idea that parasite/host interplay is critical to CD outcomes.
Descritores: Estudos de Casos e Controles
Cardiomiopatia Chagásica/complicações
Citocinas/genética
Predisposição Genética para Doença
-Interferon gama/genética
Polimorfismo de Nucleotídeo Único
Receptores Tipo I de Fatores de Necrose Tumoral
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-894865
Autor: Figueiredo, Webertty Mayk Eufrásio; Viana, Sayonara de Melo; Alves, Dorotheia Teixeira; Guerra, Priscila Valera; Coêlho, Zirlane Castelo Branco; Barbosa, Helene Santos; Teixeira, Maria Jania.
Título: Protection mediated by chemokine CXCL10 in BALB/c mice infected by Leishmania infantum
Fonte: Mem. Inst. Oswaldo Cruz;112(8):561-568, Aug. 2017. graf.
Idioma: en.
Projeto: CAPES.
Resumo: BACKGROUND Visceral leishmaniasis (VL) caused by Leishmania infantum is characterised by the loss of the ability of the host to generate an effective immune response. Chemokines have a direct involvement in the pathogenesis of leishmaniasis, causing a rapid change in the expression of these molecules during infection by Leishmania. OBJECTIVES Herein, it was investigated the role of CXCL10 in controlling infection by L. infantum. METHODS RAW 264.7 macrophages were infected with L. infantum in vitro and treated or not with CXCL10 (25, 50 and 100 ng/mL). Parasite load, as well as nitric oxide (NO), IL-4 and IL-10 production were assessed at 24 and 48 h after infection. In vivo, BALB/c mice were infected and treated or not with CXCL10 (5 μg/kg) at one, three and seven days of infection. Parasite load, IFN-g, IL-4, TGF-β and IL-10 were evaluated one, seven and 23 days post treatment. FINDINGS In vitro, CXCL10 reduced parasitic load, not dependent on NO, and inhibited IL-10 and IL-4 secretion. In vivo, CXCL10 was able to reduce the parasite load in both liver and spleen, four weeks after infection, representing a higher decrease in the number of parasites in these organs, also induced IFN-γ at day 23 after treatment, correlating with the decrease in parasite load, and reduced IL-10 and TGF-β. MAIN CONCLUSIONS This study suggests a partial protective role of CXCL10 against L. infantum, mediated by IFN-g, not dependent on NO, and with suppression of IL-10 and TGF-β. These data may provide information for the development of new approaches for future therapeutic interventions for VL.
Descritores: Tamanho do Órgão/fisiologia
Interleucina-4/biossíntese
Interleucina-10/biossíntese
Leishmania infantum
Quimiocina CXCL10/uso terapêutico
Leishmaniose Visceral/imunologia
Leishmaniose Visceral/parasitologia
Leishmaniose Visceral/tratamento farmacológico
Fígado/patologia
Macrófagos/efeitos dos fármacos
-Citocinas/imunologia
Interferon gama/análise
Camundongos Endogâmicos BALB C
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME



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