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Texto completo SciELO Brasil
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Id: biblio-837666
Autor: Zhou, Fang; Xia, Zhongyuan; Liu, Kang; Zhou, Qin.
Título: Exogenous neuregulin-1 attenuates STZ-induced diabetic peripheral neuropathic pain in rats
Fonte: Acta cir. bras;32(1):28-37, Jan. 2017. graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate whether modulating NRG1 could attenuate diabetic neuropathic pain and analyze the underlying mechanism. Methods: Male SD rats were randomly divided into control group, diabetic group, NRG1 intervention group. After STZ-induced 2 weeks, NRG1 intervention daily for consecutive 7 days. 4 weeks after NRG1 intervention, both the mechanical withdrawal threshold and the morphological changes of the dorsal root ganglion and sural nerve were observed. Meanwhile, the expression of NGF, IL-1β, TNF-α in spinal cord were determined. Results: Compared with the diabetic group, NRG1 treatment improved the mechanical withdrawal threshold in diabetic rats, pathological changes of dorsal root ganglion and sural nerve were alleviated by NRG1 treatment with electron microscopy imagine. Moreover, compared with the control group, the expression of NGF was significantly decreased and the production of IL-1β, TNF-α were markedly induced in diabetic group. Furthermore, NRG1 treatment could normalized the above effect as compared to diabetic group. Conclusion: NRG1 exerted positive effects on the behavioral and pathological changes of rats with STZ-induced diabetic neuropathic pain, the underlying mechanism might be related to the promotion of NGF excretion and the inhibition of inflammatory cytokines excretion.
Descritores: Neuregulina-1/uso terapêutico
Diabetes Mellitus Experimental/tratamento farmacológico
Neuropatias Diabéticas/tratamento farmacológico
Neuralgia/tratamento farmacológico
-Medula Espinal/metabolismo
Distribuição Aleatória
Fator de Necrose Tumoral alfa/metabolismo
Ratos Sprague-Dawley
Estreptozocina
Fator de Crescimento Neural/metabolismo
Interleucina-1beta/metabolismo
Neuralgia/etiologia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Venezuela
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Id: lil-740324
Autor: Felizzola, Ornella; Martínez, Juan Carlos; Zerpa, Noraida; Malavé, Caridad.
Título: Production and evaluation of chicken antibodies against a synthetic peptide from glial growth factor / Producción y evaluación de anticuerpos de gallinas contra un péptido sintético del factor de crecimiento glial
Fonte: Invest. clín;54(3):257-269, sep. 2013. ilus.
Idioma: en.
Resumo: Neuregulins (NRG) are proteins that belong to the family of epidermal growth factors. It is well established that these factors are essential for the development and maintenance of the nervous system. Due to the difficulty of purifying enough quantities of these factors and the lack of specificity from commercially available antibodies, the aim of this work was to produce antibodies against a synthetic peptide capable to detect and identify neuregulin GGFb isoforms. To accomplish this goal, polyclonal antibodies were raised in hens against a synthetic peptide designed from the GGFb1 extracellular sequence. The sequence analysis was made using different epitope-predicting programs. Our results showed that the peptide sequence selected was immunogenic because it was capable of inducing a specific type B immune response in the experimental animal model. These antibodies were also capable of recognizing a recombinant GGF protein and GGF isoforms present in different samples. Our results suggest that the development of immunoglobulin Y (IgY) using synthetic peptides represents, a valuable tool for neuroscience research.

Las Neuregulinas (NRG) son proteínas que pertenecen a la familia de los factores de crecimiento epidermal. Se ha demostrado que estos factores son esenciales para el desarrollo y mantenimiento de la funcionalidad del sistema nervioso. Debido a la dificultad para purificar estas proteínas y la falta de especificidad de los anticuerpos disponibles comercialmente, el objetivo de este trabajo fue producir anticuerpos contra un péptido sintético capaz de detectar e identificar una isoforma de la Neuregulina (GGFb). Para lograr este objetivo, se desarrollaron anticuerpos en gallinas (IgY) contra un péptido sintético diseñado a partir de la secuencia aminoacídica de la región extracelular de GGFb, utilizando programas de predicción de epítopes. Los resultados demuestran que el péptido seleccionado fue immunogénico debido a que estimuló una respuesta inmune específica tipo B en el modelo utilizado. Estos anticuerpos fueron también capaces de reconocer una proteína recombinante e isoformas de GGF presentes en diferentes muestras biológicas. Nuestros resultados demuestran el potencial valor de las inmunoglobulinas Y (IgY) contra péptidos sintéticos como una herramienta de aplicación para la investigación en neurociencia.
Descritores: Anticorpos Heterófilos/imunologia
Galinhas/imunologia
Imunoglobulinas/imunologia
Neuregulina-1/imunologia
Fragmentos de Peptídeos/imunologia
-Especificidade de Anticorpos
Anticorpos Heterófilos/biossíntese
Anticorpos Heterófilos/isolamento & purificação
Células Cultivadas
Meios de Cultivo Condicionados
Eletroforese em Gel de Poliacrilamida
Ensaio de Imunoadsorção Enzimática
Epitopos/imunologia
Immunoblotting
Imunoglobulinas/biossíntese
Imunoglobulinas/isolamento & purificação
Neuregulina-1/análise
Fragmentos de Peptídeos/síntese química
Isoformas de Proteínas/análise
Isoformas de Proteínas/imunologia
Ratos Sprague-Dawley
Proteínas Recombinantes/imunologia
Células de Schwann/imunologia
Células de Schwann/metabolismo
Nervo Isquiático/citologia
Limites: Animais
Feminino
Ratos
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha



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