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Id: biblio-839285
Autor: Wang, CC; Guo, L; Tian, FD; An, N; Luo, L; Hao, RH; Wang, B; Zhou, ZH.
Título: Naringenin regulates production of matrix metalloproteinases in the knee-joint and primary cultured articular chondrocytes and alleviates pain in rat osteoarthritis model
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(4):e5714, 2017. tab, graf.
Idioma: en.
Resumo: Inflammation of cartilage is a primary symptom for knee-joint osteoarthritis. Matrix metalloproteinases (MMPs) are known to play an important role in the articular cartilage destruction related to osteoarthritis. Naringenin is a plant-derived flavonoid known for its anti-inflammatory properties. We studied the effect of naringenin on the transcriptional expression, secretion and enzymatic activity of MMP-3 in vivo in the murine monosodium iodoacetate (MIA) osteoarthritis model. The assessment of pain behavior was also performed in the MIA rats. The destruction of knee-joint tissues was analyzed microscopically. Moreover, the effect of naringenin was also studied in vitro in IL-1β activated articular chondrocytes. The transcriptional expression of MMP-3, MMP-1, MMP-13, thrombospondin motifs (ADAMTS-4) and ADAMTS-5 was also studied in primary cultured chondrocytes of rats. Naringenin caused significant reduction in pain behavior and showed marked improvement in the tissue morphology of MIA rats. Moreover, a significant inhibition of MMP-3 expression in MIA rats was observed upon treatment with naringenin. In the in vitro tests, naringenin caused a significant reduction in the transcriptional expression, secretion and enzymatic activity of the studied degradative enzymes. The NF-κB pathway was also found to be inhibited upon treatment with naringenin in vitro. Overall, the study suggests that naringenin alleviated pain and regulated the production of matrix-metalloproteinases via regulation of NF-κB pathway. Thus, naringenin could be a potent therapeutic option for the treatment of osteoarthritis.
Descritores: Anti-Inflamatórios/farmacologia
Artralgia/enzimologia
Condrócitos/enzimologia
Flavanonas/farmacologia
Articulação do Joelho/enzimologia
Metaloproteinase 3 da Matriz/biossíntese
Osteoartrite do Joelho/enzimologia
-Artralgia/tratamento farmacológico
Western Blotting
Proliferação de Células/efeitos dos fármacos
Células Cultivadas
Condrócitos/efeitos dos fármacos
Modelos Animais de Doenças
Expressão Gênica
Interleucina-1beta/análise
Interleucina-1beta/efeitos dos fármacos
Interleucina-1beta/metabolismo
Articulação do Joelho/patologia
Metaloproteinase 3 da Matriz/análise
NF-kappa B/análise
NF-kappa B/efeitos dos fármacos
Inibidor de NF-kappaB alfa/análise
Inibidor de NF-kappaB alfa/efeitos dos fármacos
Osteoartrite do Joelho/tratamento farmacológico
Osteoartrite do Joelho/patologia
Distribuição Aleatória
Ratos Wistar
Reprodutibilidade dos Testes
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fatores de Tempo
Resultado do Tratamento
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME



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