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Id: biblio-954858
Autor: Valdez-Velázquez, Laura L; Olamendi-Portugal, Timoteo; Restano-Cassulini, Rita; Zamudio, Fernando Z; Possani, Lourival D.
Título: Mass fingerprinting and electrophysiological analysis of the venom from the scorpion Centruroides hirsutipalpus (Scorpiones: Buthidae)
Fonte: J. venom. anim. toxins incl. trop. dis;24:17, 2018. tab, graf, ilus.
Idioma: en.
Projeto: SEP-CONACyT; . SEP-CONACyT.
Resumo: Centruroides hirsutipalpus, of the family Buthidae, is a scorpion endemic to the Western Pacific region of Mexico. Although medically important, its venom has not yet been studied. Therefore, this communication aims to identify their venom components and possible functions. Methods Fingerprinting mass analysis of the soluble venom from this scorpion was achieved by high-performance liquid chromatography and electrospray mass spectrometry. Furthermore, the soluble venom and its toxic effects were evaluated extensively via electrophysiological assays in HEK cells expressing human voltage-gated Na+ channels (hNav 1.1 to Nav1.6), CHO cells expressing hNav 1.7, potassium channel hERG 1 (Ether-à-go-go-related-gene) and the human K+-channel hKv1.1. Results The separation of soluble venom produced 60 fractions from which 83 distinct components were identified. The molecular mass distribution of these components varies from 340 to 21,120 Da. Most of the peptides have a molecular weight between 7001 and 8000 Da (46% components), a range that usually corresponds to peptides known to affect Na+ channels. Peptides with molecular masses from 3000 to 5000 Da (28% of the components) were identified within the range corresponding to K+-channel blocking toxins. Two peptides were obtained in pure format and completely sequenced: one with 29 amino acids, showing sequence similarity to an "orphan peptide" of C. limpidus, and the other with 65 amino acid residues shown to be an arthropod toxin (lethal to crustaceans and toxic to crickets). The electrophysiological results of the whole soluble venom show a beta type modification of the currents of channels Nav1.1, Nav1.2 and Nav1.6. The main effect observed in channels hERG and hKv 1.1 was a reduction of the currents. Conclusion The venom contains more than 83 distinct components, among which are peptides that affect the function of human Na+-channels and K+-channels. Two new complete amino acid sequences were determined: one an arthropod toxin, the other a peptide of unknown function.(AU)
Descritores: Venenos de Escorpião/isolamento & purificação
Venenos de Escorpião/toxicidade
Eletrofisiologia/métodos
-Espectrometria de Massas/métodos
Sequência de Aminoácidos
Proteínas de Artrópodes/fisiologia
Limites: Animais
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: lil-643660
Autor: Cui, Yubao; Zhou, Ying; Ma, Guifang; Yang, Li; Wang, Yungang; Shi, Weihong.
Título: Cloning, bioinformatics analysis, and expression of the dust mite allergen Der f 5 of Dermatophagoides farinae
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;45(8):746-752, Aug. 2012. ilus, tab.
Idioma: en.
Resumo: Crude extracts of house dust mites are used clinically for diagnosis and immunotherapy of allergic diseases, including bronchial asthma, perennial rhinitis, and atopic dermatitis. However, crude extracts are complexes with non-allergenic antigens and lack effective concentrations of important allergens, resulting in several side effects. Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae) is one of the predominant sources of dust mite allergens, which has more than 30 groups of allergen. The cDNA coding for the group 5 allergen of D. farinae from China was cloned, sequenced and expressed. According to alignment using the VECTOR NTI 9.0 software, there were eight mismatched nucleotides in five cDNA clones resulting in seven incompatible amino acid residues, suggesting that the Der f 5 allergen might have sequence polymorphism. Bioinformatics analysis revealed that the matured Der f 5 allergen has a molecular mass of 13604.03 Da, a theoretical pI of 5.43 and is probably hydrophobic and cytoplasmic. Similarities in amino acid sequences between Der f 5 and allergens of other domestic mite species, viz. Der p 5, Blo t 5, Sui m 5, and Lep d 5, were 79, 48, 53, and 37%, respectively. Phylogenetic analysis indicated that Der f 5 and Der p 5 clustered together. Blo t 5 and Ale o 5 also clustered together, although Blomia tropicalis and Aleuroglyphus ovatus belong to different mite families, viz. Echimyopodidae and Acaridae, respectively.
Descritores: Antígenos de Dermatophagoides/genética
Proteínas de Artrópodes/genética
Dermatophagoides farinae/genética
Expressão Gênica/genética
-Sequência de Aminoácidos
Antígenos de Dermatophagoides/imunologia
Antígenos de Dermatophagoides/metabolismo
Proteínas de Artrópodes/imunologia
Proteínas de Artrópodes/metabolismo
China
Clonagem Molecular
Biologia Computacional
DNA Complementar
Dermatophagoides farinae/imunologia
Dermatophagoides farinae/metabolismo
Escherichia coli/genética
Expressão Gênica/imunologia
Dados de Sequência Molecular
Filogenia
Plasmídeos
Análise de Sequência de DNA
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-596908
Autor: Rego, Francisca X. M; Giavina-Bianchi, Pedro; Kalil, Jorge; Arruda, L. Karla; Toledo-Barros, Myrthes.
Título: The hammock: a reservoir of allergens
Fonte: Clinics;66(7):1199-1202, 2011. ilus.
Idioma: en.
Resumo: INTRODUCTION: Asthma affects approximately 10 percent of the world's population. Sensitization to allergens is an important risk factor, and exposure to allergens is associated with disease severity. METHODS: We performed skin tests to evaluate allergen sensitization to mites, cockroaches, cats, dogs, and molds in 73 asthmatic patients. Enzyme Linked Immunosorbent Assay was used to assay the mite and cockroach allergens found in dust from the bedding, hammocks, bedroom floors, living rooms, and kitchens of 29 patients and 14 controls. RESULTS: Fifty patients (68.5 percent) had positive skin test responses. There were positive responses to D. pteronyssinus (52.0 percent), B. tropicalis (53.4 percent), T. putrescentiae (15.0 percent), E. maynei (12.3 percent), L. destructor (8.2 percent), B. germanica (20.5 percent), P. americana (21.9 percent), Felis catus (10.9 percent), C. herbarium (2.7 percent), A. alternata (4.1 percent), and P. notatun (1.3 percent). The exposure to mite and cockroach allergens was similar in the patients and the controls. The Dermatophagoides pteronyssinus Group 1 levels were highest in the beds and hammocks. The Blattella germanica Group 1 levels were highest in the kitchens, living rooms and hammocks. DISCUSSION: The positive skin tests to mites, cockroaches and cats were consistent with previous studies. D pteronyssinus was the most prevalent home dust mite, and hammocks were a source of allergens. To improve asthma prophylaxis, it is important to determine its association with mite allergen exposure in hammocks.
Descritores: Alérgenos/imunologia
Asma/imunologia
Poeira/imunologia
-Antígenos de Dermatophagoides/análise
Proteínas de Artrópodes/análise
Estudos de Casos e Controles
Baratas
Cisteína Endopeptidases/análise
Poeira/análise
Ensaio de Imunoadsorção Enzimática
Ácaros
Fatores de Risco
Testes Cutâneos
Estatísticas não Paramétricas
Limites: Adolescente
Adulto
Animais
Feminino
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME



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