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Pesquisa : D12.776.097 [Categoria DeCS]
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Id: biblio-894860
Autor: Lima, Filipe R; Takenami, Iukary; Cavalcanti, Maurílio AL; Riley, Lee W; Arruda, Sérgio.
Título: ELISA-based assay of immunoglobulin G antibodies against mammalian cell entry 1A (Mce1A) protein: a novel diagnostic approach for leprosy
Fonte: Mem. Inst. Oswaldo Cruz;112(12):844-849, Dec. 2017. tab, graf.
Idioma: en.
Resumo: BACKGROUND Leprosy is a chronic infectious disease caused by the obligate intracellular bacillus Mycobacterium leprae. Because leprosy diagnosis is complex and requires professional expertise, new tools and methodologies are needed to detect cases in early stages and prevent transmission. The M. leprae genome contains mce1A, which encodes a putative mammalian cell entry protein (Mce1A). We hypothesised that the presence of Mce1A on the cell surface could be detected by the host's immune system. OBJECTIVE The aim of this study was to evaluate antibody responses against the Mce1A protein in leprosy patients, household contacts of patients, and the general population to present an addition tool for leprosy diagnosis. METHODS A cross-sectional study involving 89 volunteers [55 leprosy cases, 12 household contacts (HHC) and 22 endemic controls (EC)] was conducted at Couto Maia Hospital, in Salvador, Bahia (BA), Brazil. RESULTS The median anti-Mce1A IgA was significantly higher in multibacillary (MB) and paucibacillary (PB) cases than in EC (p < 0.0001). A similar trend was observed in IgM levels, which were significantly higher in both MB (p < 0.0001) and PB (p = 0.0006) groups compared to in EC individuals. The greatest differences were observed for IgG class-specific antibodies against Mce1A. The median levels of MB and PB were significantly higher compared to both controls HHC and EC (MB or PB vs EC, MB vs HHC p < 0.0001; PB vs HHC, p = 0.0013). Among leprosy cases, IgG enzyme-linked immunosorbent assay sensitivity and specificity were 92.7% and 97.1%, respectively. IgG positivity was confirmed in 92.1% and 94.1% of MB and PB patients, respectively. CONCLUSION This novel diagnostic approach presents an easy, non-invasive, and inexpensive method for leprosy screening, which may be applicable in endemic areas.
Descritores: Proteínas de Bactérias/imunologia
Imunoglobulina G/sangue
Hanseníase/diagnóstico
Anticorpos Antibacterianos/sangue
Mycobacterium leprae/imunologia
-Ensaio de Imunoadsorção Enzimática
Estudos de Casos e Controles
Características da Família
Sensibilidade e Especificidade
Limites: Seres Humanos
Masculino
Feminino
Adolescente
Adulto
Meia-Idade
Idoso
Responsável: BR1.1 - BIREME


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Id: biblio-889246
Autor: Saseedharan, Sanjith; Sahu, Manisa; Chaddha, Roonam; Pathrose, Edwin; Bal, Arun; Bhalekar, Pallavi; Sekar, Priyadharshini; Krishnan, Padma.
Título: Epidemiology of diabetic foot infections in a reference tertiary hospital in India
Fonte: Braz. j. microbiol;49(2):401-406, Apr.-June 2018. tab, graf.
Idioma: en.
Resumo: Abstract Introduction The present study attempts to examine the microbial profile and antibiotic susceptibility of diabetic foot infections in the intensive care unit of a tertiary referral centre for diabetic foot. As part of the study, we also attempted to find the prevalence of blaNDM-like gene among carbapenem-resistant gram negative infections. Methodology A prospective study of 261 patients with diabetic foot infections was performed during the period between January 2014 and June 2014. Results A total of 289 isolates were obtained from 178 tissue samples from 261 patients, 156 (59.7%) males and 105 (40.2%) females, with a mean age of 58 years (-15 years), having diabetic foot infection. No growth was seen in thirty eight (17.6%) tissue samples. Out of the total samples, 44.3% were monomicrobial and 55.7% were polymicrobial. Gram negative pathogens were predominant (58.5%). Seven of the total isolates were fungal; 0.7% showed pure fungal growth and 1.7% were mixed, grown along with some bacteria. The most frequently isolated bacteria were Staphylococcus aureus (26.9%), followed by Pseudomonas aeruginosa (20.9%). Of the 58.5% gram negative pathogens, 16.5% were Enterobacteriaceae resistant to carbapenems. Among these isolates, 4 (25%) were positive for blaNDM-like gene. Among the rest, 18.6% were carbapenem-resistant Pseudomonas, among which 4 (36.3%) were blaNDM. Among the Staphylococci, 23.7% were methicillin-resistant Staphylococcus aureus. Conclusions Our results support the recent view that gram negative organisms, depending on the geographical location, may be predominant in DFIs. There is an increase in multidrug-resistant pathogens, especially carbapenem resistance and this is creeping rapidly. We need to be more judicious while using empiric antibiotics.
Descritores: Infecções Bacterianas/epidemiologia
Pé Diabético/complicações
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Positivas/isolamento & purificação
Micoses/epidemiologia
-Infecções Bacterianas/microbiologia
Proteínas de Bactérias/genética
beta-Lactamases/genética
Coinfecção/epidemiologia
Coinfecção/microbiologia
Bactérias Gram-Negativas/classificação
Bactérias Gram-Positivas/classificação
Índia
Resistência a Meticilina
Testes de Sensibilidade Microbiana
Micoses/microbiologia
Prevalência
Estudos Prospectivos
Centros de Atenção Terciária
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Idoso
Responsável: BR1.1 - BIREME


  3 / 391 LILACS  
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Id: biblio-889239
Autor: Wang, Jiashun; Li, Yi; Chen, Jia; Hua, Deping; Li, Yi; Deng, Hui; Li, Ying; Liang, Zhixuan; Huang, Jinhai.
Título: Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene
Fonte: Braz. j. microbiol;49(2):320-328, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Tianjin Science and Technology.
Resumo: Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The results showed that the PagC polyclonal antiserum is of good specificity and the capture rate of 0.1 mg IMBs for Salmonella tended to be stable at the range of 70-74% corresponding to the concentrations between 101 and 104 CFU/mL. The method developed demonstrated high specificity for the positive Salmonella samples when compared to non-specific DNA samples, such as Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica, and Yersinia pseudotuberculosis. The limit of detection of this assay was 18 CFU/mL. Detection and quantitative enumeration of Salmonella in samples of pork or milk shows good recoveries of 54.34% and 52.07%. In conclusion, the polyclonal antibody of recombinant PagC protein is effective to capture Salmonella from detected samples. The developed pagC antibody IMBs-qPCR method showed efficiency, sensitivity and specificity for 30 Salmonella detection, enabling detection within 10 h, which is a promising rapid method to detect Salmonella in emergency.
Descritores: Contaminação de Alimentos
Microbiologia de Alimentos/métodos
Separação Imunomagnética/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
Salmonella/isolamento & purificação
-Anticorpos Antibacterianos/imunologia
Anticorpos Antibacterianos/metabolismo
Proteínas de Bactérias/imunologia
Carne/microbiologia
Leite/microbiologia
Salmonella/genética
Sensibilidade e Especificidade
Limites: Animais
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


  4 / 391 LILACS  
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Id: biblio-889231
Autor: Silva, Paula Renata Alves da; Simões-Araújo, Jean Luiz; Vidal, Márcia Soares; Cruz, Leonardo Magalhães; Souza, Emanuel Maltempi de; Baldani, José Ivo.
Título: Draft genome sequence of Paraburkholderia tropica Ppe8 strain, a sugarcane endophytic diazotrophic bacterium
Fonte: Braz. j. microbiol;49(2):210-211, Apr.-June 2018.
Idioma: en.
Projeto: CNPq/INCT-FBN; . FAPERJ-CNE; . Embrapa; . CAPES/EMBRAPA; . José Ivo Baldani.
Resumo: Abstract Paraburkholderia tropica (syn Burkholderia tropica) are nitrogen-fixing bacteria commonly found in sugarcane. The Paraburkholderia tropica strain Ppe8 is part of the sugarcane inoculant consortium that has a beneficial effect on yield. Here, we report a draft genome sequence of this strain elucidating the mechanisms involved in its interaction mainly with Poaceae. A genome size of approximately 8.75 Mb containing 7844 protein coding genes distributed in 526 subsystems was de novo assembled with ABySS and annotated by RAST. Genes related to the nitrogen fixation process, the secretion systems (I, II, III, IV, and VI), and related to a variety of metabolic traits, such as metabolism of carbohydrates, amino acids, vitamins, and proteins, were detected, suggesting a broad metabolic capacity and possible adaptation to plant association.
Descritores: Burkholderiaceae/genética
Endófitos/genética
Genoma Bacteriano
-Proteínas de Bactérias/genética
Burkholderiaceae/isolamento & purificação
Biologia Computacional
Endófitos/isolamento & purificação
Redes e Vias Metabólicas/genética
Anotação de Sequência Molecular
Saccharum/microbiologia
Análise de Sequência de DNA
Responsável: BR1.1 - BIREME


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Id: biblio-889211
Autor: Lima, Matheus Thomaz Nogueira Silva; Santos, Larissa Batista dos; Bastos, Rafael Wesley; Nicoli, Jacques Robert; Takahashi, Jacqueline Aparecida.
Título: Antimicrobial activity and acetylcholinesterase inhibition by extracts from chromatin modulated fungi
Fonte: Braz. j. microbiol;49(1):169-176, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of Alzheimer's disease have led to searching new control drugs. The present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. For such finality, five filamentous fungal species (Talaromyces funiculosus, Talaromyces islandicus, Talaromyces minioluteus, Talaromyces pinophilus, Penicillium janthinellum) were grown or not with DNA methyltransferases inhibitors (procainamide or hydralazine) and/or a histone deacetylase inhibitor (suberohydroxamic acid). Extracts from T. islandicus cultured or not with hydralazine inhibited Listeria monocytogenes growth in 57.66 ± 5.98% and 15.38 ± 1.99%, respectively. Increment in inhibition of acetylcholinesterase activity was observed for the extract from P. janthinellum grown with procainamide (100%), when compared to the control extract (39.62 ± 3.76%). Similarly, inhibition of acetylcholinesterase activity increased from 20.91 ± 3.90% (control) to 92.20 ± 3.72% when the tested extract was obtained from T. pinophilus under a combination of suberohydroxamic acid and procainamide. Concluding, increases in antimicrobial activity and acetylcholinesterase inhibition were observed when fungal extracts in the presence of DNA methyltransferases and/or histone deacetylase modulators were tested.
Descritores: Antibacterianos/farmacologia
Inibidores da Colinesterase/farmacologia
Penicillium/química
Talaromyces/química
-Acetilcolinesterase/química
Acetilcolinesterase/metabolismo
Antibacterianos/química
Antibacterianos/metabolismo
Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Inibidores da Colinesterase/química
Inibidores da Colinesterase/metabolismo
Cromatina/metabolismo
Listeria monocytogenes/efeitos dos fármacos
Listeria monocytogenes/enzimologia
Listeria monocytogenes/crescimento & desenvolvimento
Penicillium/metabolismo
Talaromyces/metabolismo
Responsável: BR1.1 - BIREME


  6 / 391 LILACS  
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Id: biblio-889210
Autor: Jung, Youn Hong; Lee, Yoo Kyung; Lee, Hong Kum; Lee, Kyunghee; Im, Hana.
Título: CspB of an arctic bacterium, Polaribacter irgensii KOPRI 22228, confers extraordinary freeze-tolerance
Fonte: Braz. j. microbiol;49(1):97-103, Jan.-Mar. 2018. graf.
Idioma: en.
Projeto: Korean Government.
Resumo: ABSTRACT Freezing temperatures are a major challenge for life at the poles. Decreased membrane fluidity, uninvited secondary structure formation in nucleic acids, and protein cold-denaturation all occur at cold temperatures. Organisms adapted to polar regions possess distinct mechanisms that enable them to survive in extremely cold environments. Among the cold-induced proteins, cold shock protein (Csp) family proteins are the most prominent. A gene coding for a Csp-family protein, cspB, was cloned from an arctic bacterium, Polaribacter irgensii KOPRI 22228, and overexpression of cspB greatly increased the freeze-survival rates of Escherichia coli hosts, to a greater level than any previously reported Csp. It also suppressed the cold-sensitivity of an E. coli csp-quadruple deletion strain, BX04. Sequence analysis showed that this protein consists of a unique domain at its N-terminal end and a well conserved cold shock domain at its C-terminal end. The most common mechanism of Csp function in cold adaption is melting of the secondary structures in RNA and DNA molecules, thus facilitating transcription and translation at low temperatures. P. irgensii CspB bound to oligo(dT)-cellulose resins, suggesting single-stranded nucleic acid-binding activity. The unprecedented level of freeze-tolerance conferred by P. irgensii CspB suggests a crucial role for this protein in survival in polar environments.
Descritores: Proteínas de Bactérias/metabolismo
Proteínas e Peptídeos de Choque Frio/metabolismo
Flavobacteriaceae/fisiologia
-Regiões Árticas
Proteínas de Bactérias/genética
Proteínas e Peptídeos de Choque Frio/genética
Temperatura Baixa
Ecossistema
Flavobacteriaceae/genética
Flavobacteriaceae/isolamento & purificação
Regulação Bacteriana da Expressão Gênica
Responsável: BR1.1 - BIREME


  7 / 391 LILACS  
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Id: biblio-889209
Autor: Arslan-Aydoğdu, Elif Özlem; Kimiran, Ayten.
Título: An investigation of virulence factors of Legionella pneumophila environmental isolates
Fonte: Braz. j. microbiol;49(1):189-199, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Projeto: Istanbul University.
Resumo: ABSTRACT Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase A, phospholipase C, acid phosphatase, alkaline phosphatase and lipase), hemagglutination capabilities, and pathogenicity in various host cells (Acanthamoeba castellanii ATCC 30234, mouse peritoneal macrophages and human peripheral monocytes). All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain, while 6 out of the 9 isolates (the isolates 1, 2, 3, 4, 5, and 7) were more virulent than the ATCC 33152 strain. The different bacterial strains exhibited differences in properties such as adhesion, penetration and reproduction in the hosts, and preferred host type. To our knowledge, this is the first study to compare the virulence of environmental L. pneumophila strains isolated in Turkey, and it provides important information relevant for understanding the epidemiology of L. pneumophila.
Descritores: Proteínas de Bactérias/metabolismo
Legionella pneumophila/metabolismo
Fatores de Virulência/metabolismo
-Proteínas de Bactérias/genética
Microbiologia Ambiental
Legionella pneumophila/genética
Legionella pneumophila/isolamento & purificação
Doença dos Legionários/microbiologia
Macrófagos/microbiologia
Camundongos Endogâmicos BALB C
Turquia/epidemiologia
Fatores de Virulência/genética
Limites: Seres Humanos
Animais
Feminino
Camundongos
Responsável: BR1.1 - BIREME


  8 / 391 LILACS  
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Id: biblio-889194
Autor: Ser, Hooi-Leng; Tan, Wen-Si; Ab Mutalib, Nurul-Syakima; Yin, Wai-Fong; Chan, Kok-Gan; Goh, Bey-Hing; Lee, Learn-Han.
Título: Genome sequence of Streptomyces mangrovisoli MUSC 149T isolated from intertidal sediments
Fonte: Braz. j. microbiol;49(1):13-15, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Projeto: PVC; . External Industry; . Fundamental Research Grant Scheme; . eScience; . University of Malaya; . PPP.
Resumo: ABSTRACT As the largest genus in Actinobacteria family, Streptomyces species have the ability to synthesize numerous compounds of diverse structures with bioactivities. Streptomyces mangrovisoli MUSC 149T was previously isolated as a novel streptomycete from mangrove forest in east coast of Peninsular Malaysia. The high quality draft genome of MUSC 149T comprises 9,165,825 bp with G + C content of 72.5%. Through bioinformatics analysis, 21 gene clusters identified in the genome were associated with the production of bioactive secondary metabolites. The presence of these biosynthetic gene clusters in MUSC 149T suggests the potential exploitation of the strain for production of medically important compounds.
Descritores: Genoma Bacteriano
Sedimentos Geológicos/microbiologia
Streptomyces/isolamento & purificação
-Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Composição de Bases
Sequência de Bases
DNA Bacteriano/genética
Malásia
Dados de Sequência Molecular
Filogenia
Streptomyces/classificação
Streptomyces/genética
Responsável: BR1.1 - BIREME


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Id: biblio-889189
Autor: Silva, Clara Maria Guimarães; Silva, Déborah Nascimento dos Santos; Costa, Scarlathe Bezerra da; Almeida, Juliana Soares de Sá; Boente, Renata Ferreira; Teixeira, Felipe Lopes; Domingues, Regina Maria Cavalcanti Pilotto; Lobo, Leandro Araujo.
Título: Inactivation of MarR gene homologs increases susceptibility to antimicrobials in Bacteroides fragilis
Fonte: Braz. j. microbiol;49(1):200-206, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Bacteroides fragilis is the strict anaerobic bacteria most commonly found in human infections, and has a high mortality rate. Among other virulence factors, the remarkable ability to acquire resistance to a variety of antimicrobial agents and to tolerate nanomolar concentrations of oxygen explains in part their success in causing infection and colonizing the mucosa. Much attention has been given to genes related to multiple drug resistance derived from plasmids, integrons or transposon, but such genes are also detected in chromosomal systems, like the mar (multiple antibiotic resistance) locus, that confer resistance to a range of drugs. Regulators like MarR, that control expression of the locus mar, also regulate resistance to organic solvents, disinfectants and oxygen reactive species are important players in these events. Strains derived from the parental strain 638R, with mutations in the genes hereby known as marRI (BF638R_3159) and marRII (BF638R_3706) were constructed by gene disruption using a suicide plasmid. Phenotypic response of the mutant strains to hydrogen peroxide, cell survival assay against exposure to oxygen, biofilm formation, resistance to bile salts and resistance to antibiotics was evaluated. The results showed that the mutant strains exhibit statistically significant differences in their response to oxygen stress, but no changes were observed in survival when exposed to bile salts. Biofilm formation was not affected by either gene disruption. Both mutant strains however, became more sensitive to multiple antimicrobial drugs tested. This indicates that as observed in other bacterial species, MarR are an important resistance mechanism in B. fragilis.
Descritores: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Bacteroides fragilis/efeitos dos fármacos
Bacteroides fragilis/genética
Infecções por Bacteroides/microbiologia
Proteínas Repressoras/genética
-Proteínas de Bactérias/metabolismo
Bacteroides fragilis/isolamento & purificação
Bacteroides fragilis/metabolismo
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Inativação Gênica
Testes de Sensibilidade Microbiana
Proteínas Repressoras/metabolismo
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  10 / 391 LILACS  
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Id: biblio-889188
Autor: Mares-Guia, Maria Angélica MM; Guterres, Alexandro; Rozental, Tatiana; Ferreira, Michelle dos Santos; Lemos, Elba RS.
Título: Clinical and epidemiological use of nested PCR targeting the repetitive element IS1111 associated with the transposase gene from Coxiella burnetii
Fonte: Braz. j. microbiol;49(1):138-143, Jan.-Mar. 2018. tab, graf.
Idioma: en.
Projeto: CNPq; . FAPERJ.
Resumo: ABSTRACT Q fever is a worldwide zoonosis caused by Coxiella burnetii—a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of testing while offering a suitable amplicon size for sequencing. Serial dilutions were performed tenfold to test the limit of detection, and the result was 10× detection of C. burnetti DNA with internal nested PCR primers relative to trans-PCR. Different biological samples were tested and identified only in nested PCR. This demonstrates the efficiency and effectiveness of the primers. Of the 19 samples, which amplify the partial sequence of C. burnetii, 12 were positive by conventional PCR and nested PCR. Seven samples—five spleen tissue samples from rodents and two tick samples—were only positive in nested PCR. With these new internal primers for trans-PCR, we demonstrate that our nested PCR assay for C. burnetii can achieve better results than conventional PCR.
Descritores: Proteínas de Bactérias/genética
Coxiella burnetii/isolamento & purificação
Elementos de DNA Transponíveis
Febre/microbiologia
Reação em Cadeia da Polimerase/métodos
Transposases/genética
-Proteínas de Bactérias/metabolismo
Coxiella burnetii/classificação
Coxiella burnetii/genética
Transposases/metabolismo
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME



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