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Pesquisa : D12.776.097 [Categoria DeCS]
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Id: lil-780803
Autor: Ramsugit, Saiyur; Pillay, Balakrishna; Pillay, Manormoney.
Título: Evaluation of the role of Mycobacterium tuberculosis pili (MTP) as an adhesin, invasin, and cytokine inducer of epithelial cells
Fonte: Braz. j. infect. dis;20(2):160-165, Mar.-Apr. 2016. graf.
Idioma: en.
Resumo: Abstract This study was undertaken in order to assess the involvement of Mycobacterium tuberculosis pili (MTP) as an adhesin, invasin, and cytokine inducer in the M. tuberculosis-epithelial cell interaction. A MTP-deficient strain of M. tuberculosis demonstrated a significant reduction of 69.39% (p = 0.047) and 56.20% (p = 0.033) in its ability to adhere to and invade A549 pulmonary epithelial cells, respectively, in comparison with the wild-type strain. Complementation of the MTP-deficient mutant restored its adhesion and invasion capacity back to the wild-type levels. Overall, it was found that similar concentrations of IL-1β, IL-4, IL-6, IL-8, G-CSF, IFN-γ, MCP-1, and TNF-α were induced in A549 cells infected with the MTP-proficient and MTP-deficient strains. However, at 48 h post-infection, the MTP-deficient mutant induced significantly lower levels of TNF-α than the wild-type strain (p = 0.033). Furthermore, at 72 h post-infection, the mutant induced significantly higher levels of IL-8 than the wild-type (p = 0.005). We conclude that MTP is an adhesin/invasin of epithelial cells and, while playing a role in M. tuberculosis entry, they do not appear to largely influence the epithelial cell cytokine response.
Descritores: Proteínas de Bactérias/fisiologia
Adesão Celular/fisiologia
Citocinas/imunologia
Fímbrias Bacterianas/fisiologia
Células Epiteliais/microbiologia
Mycobacterium tuberculosis/fisiologia
-Proteínas de Bactérias/metabolismo
Mycobacterium tuberculosis/imunologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-780813
Autor: Café Oliveira, Luita Nice; Muniz-Sobrinho, Jairo da Silva; Viana-Magno, Luiz Alexandre; Oliveira Melo, Sônia Cristina; Macho, Antonio; Rios-Santos, Fabrício.
Título: Detection of multidrug-resistant Mycobacterium tuberculosis strains isolated in Brazil using a multimarker genetic assay for katG and rpoB genes
Fonte: Braz. j. infect. dis;20(2):166-172, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Multidrug-resistant tuberculosis (MDRTB) is a serious world health problem that limits public actions to control tuberculosis, because the most used anti-tuberculosis first-line drugs fail to stop mycobacterium spread. Consequently, a quick detection through molecular diagnosis is essential to reduce morbidity and medical costs. Despite the availability of several molecular-based commercial-kits to diagnose multidrug-resistant tuberculosis, their diagnostic value might diverge worldwide since Mycobacterium tuberculosis genetic variability differs according to geographic location. Here, we studied the predictive value of four common mycobacterial mutations in strains isolated from endemic areas of Brazil. Mutations were found at the frequency of 41.9% for katG, 25.6% for inhA, and 69.8% for rpoB genes in multidrug-resistant strains. Multimarker analysis revealed that combination of only two mutations (“katG/S315T + rpoB/S531L”) was a better surrogate of multidrug-resistant tuberculosis than single-marker analysis (86% sensitivity vs. 62.8%). Prediction of multidrug-resistant tuberculosis was not improved by adding a third or fourth mutation in the model. Therefore, rather than using diagnostic kits detecting several mutations, we propose a simple dual-marker panel to detect multidrug-resistant tuberculosis, with 86% sensitivity and 100% specificity. In conclusion, this approach (previous genetic study + analysis of only prevalent markers) would considerably decrease the processing costs while retaining diagnostic accuracy.
Descritores: Proteínas de Bactérias/genética
RNA Polimerases Dirigidas por DNA/genética
Catalase/genética
Farmacorresistência Bacteriana Múltipla/genética
Isoniazida/farmacologia
Antituberculosos/farmacologia
-Rifampina/farmacologia
DNA Bacteriano
Testes de Sensibilidade Microbiana
Marcadores Genéticos
Reação em Cadeia da Polimerase
Valor Preditivo dos Testes
Sensibilidade e Especificidade
Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
Genótipo
Mutação/genética
Mycobacterium tuberculosis/efeitos dos fármacos
Mycobacterium tuberculosis/genética
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo de Validação
Responsável: BR1.1 - BIREME


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Id: biblio-839184
Autor: Campana, Eloiza Helena; Xavier, Danilo Elias; Petrolini, Fernanda Villas-Boas; Cordeiro-Moura, Jhonatha Rodrigo; Araujo, Maria Rita Elmor de; Gales, Ana Cristina.
Título: Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil
Fonte: Braz. j. infect. dis;21(1):57-62, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates.
Descritores: Pseudomonas aeruginosa/efeitos dos fármacos
Carbapenêmicos/farmacologia
Cefalosporinas/farmacologia
Resistência beta-Lactâmica/genética
Antibacterianos/farmacologia
-Fenótipo
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/genética
Espectrofotometria Ultravioleta
Proteínas da Membrana Bacteriana Externa
Proteínas de Bactérias/metabolismo
beta-Lactamases/metabolismo
Brasil
DNA Bacteriano
Testes de Sensibilidade Microbiana
Eletroforese em Gel de Campo Pulsado
Análise de Sequência de DNA
Porinas/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-839221
Autor: Aslam, Bilal; Khurshid, Mohsin; Nisar, Muhammad Atif; Muzammil, Saima; Hayat, Sumreen.
Título: Superantigens: procession of frets
Fonte: Braz. j. infect. dis;21(3):357-358, May-June 2017.
Idioma: en.
Descritores: Proteínas de Bactérias/imunologia
Superantígenos/imunologia
-Toxinas Bacterianas/imunologia
Superantígenos/genética
Antígenos de Bactérias/imunologia
Limites: Humanos
Animais
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: biblio-888886
Autor: Jee, Babban; Sharma, Pawan; Katoch, Kiran; Joshi, Beenu; Awasthi, Sudhir Kumar.
Título: IL-10 down-regulates the expression of survival associated gene hspX of Mycobacterium tuberculosis in murine macrophage
Fonte: Braz. j. infect. dis;21(4):386-390, July-Aug. 2017. graf.
Idioma: en.
Resumo: Abstract Mycobacterium tuberculosis (MTB) adopts a special survival strategy to overcome the killing mechanism(s) of host immune system. Amongst the many known factors, small heat shock protein 16.3 (sHSP16.3) of MTB encoded by gene hspX has been reported to be critical for the survival of MTB. In the present study, the effect of recombinant murine interferon-gamma (rmIFN-γ) and recombinant murine interleukin-10 (rmIL-10) on the expression of gene hspX of MTB in murine macrophage RAW264.7 has been investigated. By real-time RT-PCR, it was observed that three increasing concentrations (5, 25 and 50 ng/ml) of rmIFN-γ significantly up-regulated the expression of hspX whereas similar concentrations of rmIL-10 (5, 25 and 50 ng/ml) significantly down-regulated the hspX expression. This effect was not only dependent on the concentration of the stimulus but this was time-dependent as well. A contrasting pattern of hspX expression was observed against combinations of two different concentrations of rmIFN-γ and rmIL-10. The study results suggest that rIL-10 mediated down-regulation of hspX expression, in the presence of low concentration of rIFN-γ, could be used as an important strategy to decrease the dormancy of MTB in its host and thus making MTB susceptible to the standard anti-mycobacterial therapy used for treating tuberculosis. However, as these are only preliminary results in the murine cell line model, this hypothesis needs to be first validated in human cell lines and subsequently in animal models mimicking the latent infection using clinical isolates of MTB before considering the development of modified regimens for humans.
Descritores: Proteínas de Bactérias/metabolismo
Regulação Bacteriana da Expressão Gênica/fisiologia
Interferon gama/farmacologia
Interleucina-10/farmacologia
Macrófagos/microbiologia
Mycobacterium tuberculosis/genética
Antígenos de Bactérias/metabolismo
-Fatores de Tempo
Proteínas de Bactérias/genética
Proteínas Recombinantes/farmacologia
Regulação para Baixo/efeitos dos fármacos
Relação Dose-Resposta a Droga
Antígenos de Bactérias/genética
Responsável: BR1.1 - BIREME


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Id: biblio-1039207
Autor: Alves, Geraldo da Silva; Pereira, Monalessa Fábia; Bride, Lais de Lima; Nunes, Ana Paula Ferreira; Schuenck, Ricardo Pinto.
Título: Clonal dissemination of vancomycin-resistant Enterococcus faecium ST412 in a Brazilian region
Fonte: Braz. j. infect. dis;21(6):656-659, Nov.-Dec. 2017. graf.
Idioma: en.
Resumo: ABSTRACT Vancomycin-resistant Enterococcus faecium (VREfm) has emerged as an important global nosocomial pathogen, and this trend is associated with the spread of high-risk clones. Here, we determined the genetic and phenotypic features of 93 VREfm isolates that were obtained from patients in 13 hospitals in Vitória, Espírito Santo, Brazil, during 2012-2013. All the isolates were vancomycin-resistant and harbored the vanA gene. Only 6 (6.5%) of the VREfm isolates showed the ability to form biofilm. The 93 isolates analyzed belong to a single pulsed-field gel electrophoresis lineage and presented six subtypes. MLST genotyping showed that all VREfm belonged to ST412 (the high-risk clone, hospital-adapted). The present study describes the dissemination of ST412 clone in the local hospitals. The clonal spread of these ST412 isolates in the area we analyzed as well as other hospitals in southeastern Brazil supports the importance of identifying and controlling the presence of these microorganisms in health care-related services.
Descritores: Infecção Hospitalar/microbiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Enterococcus faecium/genética
Enterococos Resistentes à Vancomicina/genética
-Proteínas de Bactérias
Brasil
Testes de Sensibilidade Microbiana
Técnicas de Tipagem Bacteriana
Enterococcus faecium/efeitos dos fármacos
Eletroforese em Gel de Campo Pulsado
Tipagem de Sequências Multilocus
Enterococos Resistentes à Vancomicina/efeitos dos fármacos
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1039209
Autor: Barberino, Maria Goreth; Cruvinel, Silvia de Araujo; Faria, Célio; Salvino, Marco Aurélio; Silva, Marcio de Oliveira.
Título: Isolation of blaNDM-producing Enterobacteriaceae in a public hospital in Salvador, Bahia, Brazil
Fonte: Braz. j. infect. dis;22(1):47-50, Jan.-feb. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Carbapenemases have great importance in the global epidemiological scenario since infections with carbapenemase-producing bacteria are associated with high mortality, especially in hospitalized patients in intensive care units. This study describes two microorganisms producers of the New Delhi Metallo-b-lactamase, Klebsiella pneumoniae and Citrobacter freundii, from two patients admitted to a public hospital in Salvador, Bahia. These are the first clinical cases of New Delhi Metallo-b-lactamase described in microorganisms in the north and northeast Brazil. The isolates were characterized by antimicrobial susceptibility test, with resistance to all β-lactams including carbapenems, negative Modified Hodge Test and the synergy test with Ethylenediaminetetraacetic acid, Phenylboronic Acid and Cloxacillin was positive only with Ethylenediaminetetraacetic acid (difference of >5 mm in the inhibition zone between the disk without and with the inhibitor). Analysis by multiplex PCR for blaIMP, blaVIM, blaNDM, blaKPC and blaOXA-48 enzymes confirmed the presence of blaNDM gene. This report of two different New Delhi Metallo-b-lactamase-producing microorganisms in a different region of Brazil confirms the risk of spreading resistance genes between different species and emphasizes the need for prevention and control of infections caused by these pathogens, which have limited treatment options and have been linked to high mortality rates.
Descritores: Proteínas de Bactérias/metabolismo
beta-Lactamases/metabolismo
Enterobacteriaceae/enzimologia
Infecções por Enterobacteriaceae/microbiologia
-Proteínas de Bactérias/efeitos dos fármacos
beta-Lactamases/efeitos dos fármacos
Brasil
Carbapenêmicos/farmacologia
Evolução Fatal
Farmacorresistência Bacteriana
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/efeitos dos fármacos
Reação em Cadeia da Polimerase Multiplex
Hospitais Públicos
Limites: Humanos
Masculino
Adulto
Idoso
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-951629
Autor: Rocchetti, Taisa Trevizani; Martins, Katheryne Benini; Martins, Patricia Yoshida Faccioli; Oliveira, Rogério Antonio de; Mondelli, Alessandro Lia; Fortaleza, Carlos Magno Castelo Branco; Cunha, Maria de Lourdes Ribeiro de Souza da.
Título: Detection of the mecA gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction
Fonte: Braz. j. infect. dis;22(2):99-105, Mar.-Apr. 2018. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: ABSTRACT Introduction: Staphylococcus spp. - both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) - are relevant agents of healthcare-associated infections. Therefore, the rapid recognition of MRSA and methicillin-resistant CoNS from blood stream infections is critically important for patient management. It is worth noting that inappropriate empiric therapy has been associated with higher in-hospital mortality. Material and methods: In this study we evaluated a multiplex polymerase chain reaction (multiplex PCR) standardized to detect Staphylococcus spp., S. aureus, and mecA gene-encoded oxacillin resistance directly from blood culture bottles. A total of 371 blood cultures with Gram-positive microorganisms confirmed by Gram-stain were analyzed. Results from multiplex PCR were compared to phenotypic characterization of isolates. Results: Staphylococcus aureus was detected in 85 (23.0%) blood cultures and CoNS in 286 (77.0%). There was 100% agreement between phenotypic and multiplex PCR identification. Forty-three (50.6%) of the 85 S. aureus carried the mecA gene and among the 286 CoNS, 225 (78.7%) were positive for the mecA gene. Conclusions: The multiplex PCR assay developed here was found to be sensitive, specific, rapid, and showed good agreement with the phenotypic results besides being less expensive. This PCR method could be used in clinical laboratories for rapid identification and initiation of specific and effective treatment, reducing patient mortality and morbidity. Furthermore, this method may reduce misuse of antimicrobial classes that are more expensive and toxic, thus contributing to the selection of antibiotic-resistant Staphylococcus spp.
Descritores: Proteínas de Bactérias/genética
Sangue/microbiologia
Bacteriemia/diagnóstico
Proteínas de Ligação às Penicilinas/genética
Staphylococcus aureus Resistente à Meticilina/genética
Reação em Cadeia da Polimerase Multiplex
-Oxacilina/farmacologia
Infecções Estafilocócicas/diagnóstico
Staphylococcus aureus/isolamento & purificação
Staphylococcus aureus/efeitos dos fármacos
Proteínas de Bactérias/isolamento & purificação
DNA Bacteriano/genética
Bacteriemia/microbiologia
Proteínas de Ligação às Penicilinas/isolamento & purificação
Hemocultura
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-951633
Autor: Lima, Jailton Lobo da Costa; Alves, Lilian Rodrigues; Jacomé, Paula Regina Luna de Araújo; Bezerra Neto, João Pacífico; Maciel, Maria Amélia Vieira; Morais, Marcia Maria Camargo de.
Título: Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing
Fonte: Braz. j. infect. dis;22(2):129-136, Mar.-Apr. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Introduction: Biofilm production is an important mechanism for the survival of Pseudomonas aeruginosa and its relationship with antimicrobial resistance represents a challenge for patient therapeutics. P. aeruginosa is an opportunistic pathogen frequently associated to nosocomial infections, especially in imunocompromised hosts. Objectives: Analyze the phenotypic biofilm production in P. aeruginosa isolates, describe clonal profiles, and analyze quorum sensing (QS) genes and the occurrence of mutations in the LasR protein of non-biofilm producing isolates. Methods: Isolates were tested for biofilm production by measuring cells adherence to the microtiter plates. Clonal profile analysis was carried out through ERIC-PCR, QS genes were by specific PCR. Results: The results showed that 77.5% of the isolates were considered biofilm producers. The results of genotyping showed 38 distinct genetic profiles. As for the occurrence of the genes, 100% of the isolates presented the lasR, rhlI and rhlR genes, and 97.5%, presented the lasI gene. In this study nine isolates were not biofilm producers. However, all presented the QS genes. Amplicons related to genes were sequenced in three of the nine non-biofilm-producing isolates (all presenting different genetic similarity profile) and aligned to the sequences of those genes in P. aeruginosa strain PAO1 (standard biofilm-producing strain). Alignment analysis showed an insertion of three nucleotides (T, C and G) causing the addition of an amino acid valine in the sequence of the LasR protein, in position 53. Conclusion: The modeling of the resulting LasR protein showed a conformational change in its structure, suggesting that this might be the reason why these isolates are unable to produce biofilm.
Descritores: Pseudomonas aeruginosa/fisiologia
Infecções por Pseudomonas/microbiologia
Proteínas de Bactérias/genética
Transativadores/genética
Biofilmes/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
-Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/química
Infecções por Pseudomonas/tratamento farmacológico
Proteínas de Bactérias/química
Transativadores/química
Reação em Cadeia da Polimerase/métodos
Infecção Hospitalar
Farmacorresistência Bacteriana Múltipla
Anti-Infecciosos/farmacologia
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-984018
Autor: Chen, Jiazhen; Ruan, Qiaoling; Shen, Yaojie; Wang, Sen; Shao, Lingyun; Zhang, Wenhong.
Título: Assessing and screening for T-cell epitopes from Mycobacterium tuberculosis RD2 proteins for the diagnosis of active tuberculosis
Fonte: Braz. j. infect. dis;22(6):462-471, Nov.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Major Project of the Twelfth Five-Year Plan; . National Natural Science Foundation of China; . Shanghai Science and Technology Commission.
Resumo: ABSTRACT The Region of D eletion 2 (RD2) of Mycobacterium tuberculosis encodes reserved antigens that contribute to bacterial virulence. Among these antigens, Rv1983, Rv1986, Rv1987, and Rv1989c have been shown to be immunodominant in infected cattle; however, their diagnostic utility has not been evaluated in humans.In this study, we screened 87 overlapping synthetic peptides encoded by five RD2 proteins for diagnosing tuberculosis epitopes in 50 active tuberculosis (TB) cases, 31 non-tuberculosis patients and 36 healthy individuals. A pool of promising epitopes was then assessed for their diagnostic value in 233 suspected TB patients using a whole blood IFN-γ release assay.Only 10 peptides were recognized by more than 10% of active tuberculosis patients. The IFN-γ release responses to Rv1986-P9, P15, P16, Rv1988-P4, P11, and Rv1987-P11 were significantly higher in the active TB group than in the control groups (p < 0.05). The whole blood IFN-γ release assay based on these epitopes yielded a sensitivity of 51% and a specificity of 85% in diagnosing active tuberculosis, and the corresponding results using the T-SPOT.TB assay were 76% and 75%, respectively.In conclusion, these results suggest that the six epitopes from the RD2 of M. tuberculosis have potential diagnostic value in TB.
Descritores: Proteínas de Bactérias/imunologia
Tuberculose/diagnóstico
Epitopos de Linfócito T/imunologia
Mycobacterium tuberculosis/imunologia
Antígenos de Bactérias/imunologia
-Proteínas de Bactérias/sangue
Tuberculose/imunologia
Tuberculose Pulmonar/diagnóstico
Tuberculose Pulmonar/imunologia
Estudos de Casos e Controles
Estudos Retrospectivos
Sensibilidade e Especificidade
Epitopos de Linfócito T/sangue
Antígenos de Bactérias/sangue
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME



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