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Pesquisa : D12.776.097 [Categoria DeCS]
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Stefani, Mariane Martins de Araujo
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Id: biblio-1085423
Autor: Hungria, Emerith Mayra; Oliveira, Regiane Morillas de; Souza, Ana Lúcia Osório Maroclo de; Costa, Maurício Barcelos; Souza, Vânia Nieto Brito de; Silva, Eliane Aparecida; Vilani-Moreno, Fátima Regina; Nogueira, Maria Esther Salles; Costa, Maria Renata Sales Nogueira; Silva, Sônia Maria Usó Ruiz; Bührer-Sékula, Samira; Reed, Steven G; Duthie, Malcolm S; Stefani, Mariane Martins de Araújo.
Título: Seroreactivity to new Mycobacterium leprae protein antigens in different leprosy-endemic regions in Brazil.
Fonte: Rio de Janeiro; s.n; 2012. 8 p. ilus, map, tab, graf.
Idioma: en.
Resumo: New Mycobacterium leprae protein antigens can contribute to improved serologic tests for leprosy diagnosis/classification and multidrug therapy (MDT) monitoring. This study describes seroreactivity to M. leprae proteins among participants from three highly endemic leprosy areas in Brazil: central-western Goiânia/Goiás (GO) (n = 225), Rondonópolis/Mato Grosso (MT) (n = 764) and northern Prata Village/Pará (PA) (n = 93). ELISA was performed to detect IgG to proteins (92f, 46f, leprosy IDRI diagnostic-1, ML0405, ML1213) and IgM to phenolic glycolipid-I (PGL-I). Multibacillary (MB) leprosy had positive rates for PGL-I that were similar to those for proteins; however, some anti-PGL-I-negative subjects were positive for proteins, suggesting that adding protein antigen to PGL-I can enhance the sensitivity of MB leprosy detection. In MT, different degrees of seroreactivity were observed and ranked for MB, former patients after MDT, paucibacillary (PB) leprosy, household contact (HHC) and endemic control (EC) groups. The seroreactivity of PB patients was low in GO and MT. HHCs from different endemic sites had similar IgG antibody responses to proteins. 46f and 92f were not recognised by most tuberculosis patients, ECs or HHCs within GO, an area with high BCG vaccination coverage. Low positivity in EC and HHC was observed in PA and MT. Our results provide evidence for the development of an improved serologic test that could be widely applicable for MB leprosy testing in Brazil
Descritores: Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/sangue
Doenças Endêmicas
Glicolipídeos/sangue
Hanseníase/diagnóstico
Hanseníase/epidemiologia
Mycobacterium leprae/imunologia
Proteínas de Bactérias/sangue
-Brasil/epidemiologia
Ensaio de Imunoadsorção Enzimática
Estudos de Casos e Controles
Imunoglobulina M/sangue
Limites: Humanos
Masculino
Feminino
Adulto
Responsável: BR191.1 - Biblioteca e Centro de Documentação Luiza Keffer
BR191.1; 9462/s


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Id: biblio-951820
Autor: Bachir, Meryem; Allem, Rachida; Tifrit, Abedelkarim; Medjekane, Meriem; Drici, Amine El-Mokhtar; Diaf, Mustafa; Douidi, Kara Turki.
Título: Primary antibiotic resistance and its relationship with cagA and vacA genes in Helicobacter pylori isolates from Algerian patients
Fonte: Braz. j. microbiol;49(3):544-551, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: Algerian Research Ministry.
Resumo: Abstract The epidemiology of Helicobacter pylori resistance to antibiotics is poorly documented in Africa and especially in Algeria. The aim of our study was to determine the antibiotic resistance rates, as well as its possible relationship with VacA and CagA virulence markers of isolates from Algerian patients. One hundred and fifty one H. pylori isolate were obtained between 2012 and 2015 from 200 patients with upper abdominal pain. Antimicrobial susceptibility testing was performed for amoxicillin, clarithromycin, metronidazole, ciprofloxacin, rifampicin and tetracycline. Molecular identification of H. pylori and the detection of vacA and cagA genes were performed using specific primers. We found that H. pylori was present in 83.5% of collected biopsies, 54.9% of the samples were cagA positive, 49.67% were vacA s1m1, 18.30% were vacA s1m2 and 25.49% were vacA s2m2. Isolates were characterized by no resistance to amoxicillin (0%), tetracycline (0%), rifampicin (0%), a high rate of resistance to metronidazole (61.1%) and a lower rate of resistance to clarithromycin (22.8%) and ciprofloxacin (16.8%). No statically significant relationship was found between vagA and cagA genotypes and antibiotic resistance results (p > 0.5) except for the metronidazole, which had relation with the presence of cagA genotype (p = 0.001).
Descritores: Proteínas de Bactérias/genética
Helicobacter pylori/efeitos dos fármacos
Infecções por Helicobacter/microbiologia
Farmacorresistência Bacteriana
Antibacterianos/farmacologia
Antígenos de Bactérias/genética
-Helicobacter pylori/isolamento & purificação
Helicobacter pylori/genética
Helicobacter pylori/metabolismo
Claritromicina/farmacologia
Argélia
Amoxicilina/farmacologia
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-951814
Autor: Ghods, Nayereh; Falahati, Mehraban; Roudbary, Maryam; Farahyar, Shirin; Shamaei, Masoud; Pourabdollah, Mahin; Seif, Farhad.
Título: Differential role of gpaB and sidA gene expressions in relation to virulence in Aspergillus species from patients with invasive aspergillosis
Fonte: Braz. j. microbiol;49(3):668-674, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: University of Medical Sciences.
Resumo: Abstract The virulence genes in invasive aspergillosis (IA) have not been analyzed adequately. The present study was designed to evaluate the expression of gpaB and sidA genes, which are important virulence genes in Aspergillus spp. from bronchoalveolar lavage (BAL) samples. Direct examination and culture on Czapek Agar and Sabouraud Dextrose Agar media were performed for 600 BAL specimens isolated from patients with possible aspergillosis. A Galactomannan ELISA assay was also carried out. The expression levels of the gpaB and sidA genes in isolates were analyzed using quantitative real-time PCR (qRT-PCR). We identified 2 species, including Aspergillus flavus (A. flavus) and Aspergillus fumigatus (A. fumigatus) in 25 positive samples for invasive aspergillosis as validated using GM-ELISA. A. flavus is the main pathogen threatening transplant recipients and cancer patients worldwide. In this study, A. flavus had low levels of the gpaB gene expression compared to A. fumigatus (p = 0.006). The highest sidA expression was detected in transplant recipients (p = 0.05). There was no significant correlation between sidA expression and underlying disease (p = 0.15). The sidA and gpaB gene expression patterns may provide evidence that these virulence genes play important roles in the pathogenicity of Aspergillus isolates; however, there are several regulatory genes responsible for the unexpressed sidA and gpaB genes in the isolates.
Descritores: Aspergilose/microbiologia
Aspergillus flavus/metabolismo
Aspergillus flavus/patogenicidade
Aspergillus fumigatus/metabolismo
Aspergillus fumigatus/patogenicidade
Proteínas de Bactérias/metabolismo
-Aspergillus flavus/isolamento & purificação
Aspergillus flavus/genética
Aspergillus fumigatus/isolamento & purificação
Aspergillus fumigatus/genética
Proteínas de Bactérias/genética
Virulência
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


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Id: biblio-951806
Autor: Alves, Lucas Bocchini Rodrigues; Freitas Neto, Oliveiro Caetano de; Batista, Diego Felipe Alves; Barbosa, Fernanda de Oliveira; Rubio, Marcela da Silva; Souza, Andrei Itajahy Secundo de; Almeida, Adriana Maria de; Barrow, Paul Andrew; Berchieri Junior, Angelo.
Título: Inactivation of phoPQ genes attenuates Salmonella Gallinarum biovar Gallinarum to susceptible chickens
Fonte: Braz. j. microbiol;49(3):601-606, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation.
Resumo: Abstract Salmonella Gallinarum is a host-restrict pathogen that causes fowl typhoid, a severe systemic disease that is one of the major concerns to the poultry industry worldwide. When infecting the bird, SG makes use of evasion mechanisms to survive and to replicate within macrophages. In this context, phoPQ genes encode a two-component regulatory system (PhoPQ) that regulates virulence genes responsible for adaptation of Salmonella spp. to antimicrobial factors such as low pH, antimicrobial peptides and deprivation of bivalent cations. The role of the mentioned genes to SG remains to be investigated. In the present study a phoPQ-depleted SG strain (SG ΔphoPQ) was constructed and its virulence assessed in twenty-day-old laying hens susceptible to fowl typhoid. SG ΔphoPQ did cause neither clinical signs nor mortality in birds orally challenged, being non-pathogenic. Furthermore, this strain was not recovered from livers or spleens. On the other hand, chickens challenged subcutaneously with the mutant strain had discreet to moderate pathological changes and also low bacterial counts in liver and spleen tissues. These findings show that SG ΔphoPQ is attenuated to susceptible chickens and suggest that these genes are important during chicken infection by SG.
Descritores: Doenças das Aves Domésticas/microbiologia
Salmonelose Animal/microbiologia
Proteínas de Bactérias/genética
Salmonella enterica/metabolismo
Salmonella enterica/patogenicidade
Inativação Gênica
-Doenças das Aves Domésticas/patologia
Salmonelose Animal/patologia
Baço/microbiologia
Baço/patologia
Proteínas de Bactérias/metabolismo
Virulência
Galinhas
Salmonella enterica/genética
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME


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Id: biblio-974322
Autor: Pontes, Patricia Silveira de; Coutinho, Selene Dall' Acqua; Iovine, Renata de Oliveira; Cunha, Marcos Paulo Vieira; Knöbl, Terezinha; Carvalho, Vania Maria de.
Título: Survey on pathogenic Escherichia coli and Salmonella spp. in captive cockatiels (Nymphicus hollandicus)
Fonte: Braz. j. microbiol;49(supl.1):76-82, 2018. tab, graf.
Idioma: en.
Resumo: Abstract We surveyed healthy captive cockatiels (Nymphicus hollandicus) for Escherichia coli and Salmonella spp. Cloacal swabs were collected from 94 cockatiels kept in commercial breeders, private residencies and pet shops in the cities of São Paulo/SP and Niterói/RJ (Brazil). Three strains of E. coli from each individual were tested for the presence of ExPEC-, APEC- and DEC-related genes. We evaluated the blaTEM, blaSHV, blaOXA, blaCMY, blaCTX-M, tetA, tetB, aadA, aphA, strAB, sul1, sul2, sul3, qnrA, qnrD, qnrB, qnrS, oqxAB, aac (6)′-Ib-cr, qepA resistance genes and markers for plasmid incompatibility groups. Salmonella spp. was not detected. E. coli was isolated in 10% of the animals (9/94). Four APEC genes (ironN, ompT, iss and hlyF) were detected in two strains (2/27-7%), and iss (1/27-4%) in one isolate. The highest resistance rates were observed with amoxicillin (22/27-82%), ampicillin (21/27-79%), streptomycin (18/27-67%), tetracycline (11/27-41%). Multiresistance was verified in 59% (16/27) of the isolates. We detected strAB, bla TEM, tetA, tetB, aadA, aphaA, sul1, sul2, sul3 resistance genes and plasmid Inc groups in 20 (74%) of the strains. E. coli isolated from these cockatiels are of epidemiological importance, since these pets could transmit pathogenic and multiresistant microorganisms to humans and other animals.
Descritores: Salmonella/isolamento & purificação
Salmonelose Animal/microbiologia
Doenças das Aves/microbiologia
Cacatuas/microbiologia
Escherichia coli/isolamento & purificação
Infecções por Escherichia coli/veterinária
-Plasmídeos/genética
Plasmídeos/metabolismo
Salmonella/classificação
Salmonella/fisiologia
Salmonella/genética
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Brasil
Farmacorresistência Bacteriana Múltipla
Escherichia coli/classificação
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Infecções por Escherichia coli/microbiologia
Antibacterianos/farmacologia
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-974312
Autor: Sagiroglu, Pinar; Hasdemir, Ufuk; Gelmez, Gülen Altinkanat; Aksu, Burak; Karatuna, Onur; Söyletir, Güner.
Título: Performance of "RESIST-3 O.K.N. K-SeT" immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey
Fonte: Braz. j. microbiol;49(4):885-890, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Marmara University Clinical Research Ethics Committee.
Resumo: ABSTRACT In this study, the performance of the "RESIST-3 O.K.N. K-SeT" (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla KPC, bla IMP, bla VIM, bla NDM, and bla OXA-48. The rates of bla NDM, bla OXA-48, and bla KPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla NDM and bla OXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132 K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying bla KPC, bla NDM, and/or bla OXA-48. On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring bla IMP and bla VIM. We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories.
Descritores: Proteínas de Bactérias/análise
beta-Lactamases/análise
Infecções por Klebsiella/microbiologia
Imunoensaio/métodos
Klebsiella pneumoniae/enzimologia
-Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Turquia
beta-Lactamases/metabolismo
Carbapenêmicos/farmacologia
Reação em Cadeia da Polimerase
Klebsiella pneumoniae/isolamento & purificação
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/química
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-974300
Autor: Sundarrajan, Sudarson; Rao, Sneha; Padmanabhan, Sriram.
Título: Cloning and high-level expression of Thermus thermophilus RecA in E. coli: purification and novel use in HBV diagnostics
Fonte: Braz. j. microbiol;49(4):848-855, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was purified using a single heat treatment step without the use of any chromatography steps, and the purified protein (>95%) was found to be active. The purified RecA could enhance the polymerase chain reaction (PCR) signals of HBV and improve the detection limit of the HBV diagnosis by real time PCR. The yield of recombinant RecA was ∼35 mg/L, the highest yield reported for a recombinant RecA to date. RecA can be successfully employed to enhance detection sensitivity for the diagnosis of DNA viruses such as HBV, and this methodology could be particularly useful for clinical samples with HBV viral loads of less than 10 IU/mL, which is interesting and novel.
Descritores: Proteínas de Bactérias/genética
Vírus da Hepatite B/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Thermus thermophilus/enzimologia
Clonagem Molecular
Recombinases/genética
-Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/metabolismo
Expressão Gênica
Vírus da Hepatite B/genética
Reação em Cadeia da Polimerase/instrumentação
Thermus thermophilus/genética
Recombinases/isolamento & purificação
Recombinases/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-974291
Autor: Agunbiade, Mayowa; Pohl, Carolina; Ashafa, Omotayo.
Título: Bioflocculant production from Streptomyces platensis and its potential for river and waste water treatment
Fonte: Braz. j. microbiol;49(4):731-741, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank as Streptomyces platensis with accession number FJ 486385.1. Culture conditions for optimal production of the bioflocculant included glucose as a sole carbon source, resulting in flocculating activity of 90%. Other optimal conditions included: peptone as nitrogen source; presence of Mg2+ as cations and inoculum size of 1.0% (v/v) at neutral pH of 7. Optimum dose of the purified bioflocculant for the clarification of 4 g/L kaolin clay suspension at neutral pH was 0.2 mg/mL. Energy Dispersive X-ray analysis confirmed elemental composition of the purified bioflocculant in mass proportion (%w/w): carbon (21.41), oxygen (35.59), sulphur (26.16), nitrogen (0.62) and potassium (7.48). Fourier Transform Infrared Spectroscopy (FTIR) indicated the presence of hydroxyl, carboxyl, methoxyl and amino group in the bioflocculant. The bioflocculant produced by S. platensis removed chemical oxygen demand (COD) in river water and meat processing wastewater at efficiencies of 63.1 and 46.6% respectively and reduced their turbidity by 84.3 and 75.6% respectively. The high flocculating rate and removal efficiencies displayed by S. platensis suggests its industrial application in wastewater treatment.
Descritores: Streptomyces/química
Proteínas de Bactérias/metabolismo
Águas Residuárias/química
-Streptomyces/isolamento & purificação
Streptomyces/genética
Streptomyces/metabolismo
Proteínas de Bactérias/genética
Microbiologia da Água
Carbono/metabolismo
Purificação da Água
Rios/química
Floculação
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  9 / 417 LILACS  
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Barth, Afonso Luís
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Id: biblio-974286
Autor: Pancotto, Lisiane Rech; Nodari, Carolina Silva; Rozales, Franciéli Pedrotti; Soldi, Tatiane; Siqueira, Carolina Gomes; Freitas, Ana Lúcia; Barth, Afonso Luís.
Título: Performance of rapid tests for carbapenemase detection among Brazilian Enterobacteriaceae isolates
Fonte: Braz. j. microbiol;49(4):914-918, Oct.-Dec. 2018. tab.
Idioma: en.
Resumo: ABSTRACT The global emergence of carbapenemases led to the need of developing new methods for their rapid detection. The aim of this study was to evaluate the performance of the rapid tests for carbapenemase-producing and non-producing Enterobacteriaceae. Carbapenem non-susceptible Enterobacteriaceae from a surveillance study submitted to a multiplex real time PCR for carbapenemase detection were included in this study. The isolates were subjected to the rapid phenotypic tests Carba NP, Blue-Carba and Carbapenem Inactivation Method (CIM). A total of 83 carbapenemase-producing (43) and non-producing (40) isolates were included in the study. The sensitivity/specificity were 62.7%/97.5%, 95.3%/100%, and 74.4%/97.5% for Carba NP, Blue-Carba and CIM, respectively. Both Carba NP and Blue-Carba presented their final results after 75 min of incubation; the final results for CIM were obtained only after 8 h. Failure to detect OXA-370 carbapenemase was the main problem for Carba NP and CIM assays. As the Blue-Carba presented the highest sensitivity, it can be considered the best screening test. Conversely, CIM might be the easiest to perform, as it does not require special reagents. The early detection of carbapenemases aids to establish infection control measures and prevent carbapenemases to spread reducing the risk of healthcare associated infections and therapeutic failure.
Descritores: Proteínas de Bactérias/análise
beta-Lactamases/análise
Enterobacteriaceae/enzimologia
Infecções por Enterobacteriaceae/microbiologia
Ensaios Enzimáticos/métodos
-Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
beta-Lactamases/genética
beta-Lactamases/metabolismo
Brasil
Carbapenêmicos/farmacologia
Reação em Cadeia da Polimerase
Sensibilidade e Especificidade
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/efeitos dos fármacos
Enterobacteriaceae/genética
Infecções por Enterobacteriaceae/diagnóstico
Antibacterianos/farmacologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1039272
Autor: Azevedo, Paola Aparecida Alves; Furlan, João Pedro Rueda; Oliveira-Silva, Mariana; Nakamura-Silva, Rafael; Gomes, Carolina Nogueira; Costa, Karen Regina Carim; Stehling, Eliana Guedes; Pitondo-Silva, André.
Título: Detection of virulence and ß-lactamase encoding genes in Enterobacter aerogenes and Enterobacter cloacae clinical isolates from Brazil
Fonte: Braz. j. microbiol;49(supl.1):224-228, 2018. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation - FAPESP.
Resumo: ABSTRACT Enterobacter cloacae and E. aerogenes have been increasingly reported as important opportunistic pathogens. In this study, a high prevalence of multi-drug resistant isolates from Brazil, harboring several β-lactamase encoding genes was found. Several virulence genes were observed in E. aerogenes, contrasting with the E. cloacae isolates which presented none.
Descritores: Proteínas de Bactérias/metabolismo
beta-Lactamases/metabolismo
Enterobacter cloacae/isolamento & purificação
Enterobacter aerogenes/isolamento & purificação
Fatores de Virulência/metabolismo
Infecções por Enterobacteriaceae/microbiologia
-Filogenia
Proteínas de Bactérias/genética
Virulência
beta-Lactamases/genética
Brasil
Testes de Sensibilidade Microbiana
Enterobacter cloacae/classificação
Enterobacter cloacae/enzimologia
Enterobacter cloacae/genética
Enterobacter aerogenes/classificação
Enterobacter aerogenes/enzimologia
Enterobacter aerogenes/genética
Fatores de Virulência/genética
Pessoa de Meia-Idade
Antibacterianos/farmacologia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME



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