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Reiche, Edna Maria Vissoci
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Id: biblio-829121
Autor: Capobiango, Jaqueline Dario; Monica, Thaís Cabral; Ferreira, Fernanda Pinto; Mitsuka-Breganó, Regina; Navarro, Italmar Teodorico; Garcia, João Luis; Reiche, Edna Maria Vissoci.
Título: Evaluation of the Western blotting method for the diagnosis of congenital toxoplasmosis / Avaliação do método Western Blotting para diagnóstico de toxoplasmose congênita
Fonte: J. pediatr. (Rio J.);92(6):616-623, Nov.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Objective: To evaluate the Western blotting method for the detection of IgG anti-Toxoplasma gondii (T. gondii) (IgG-WB) in the serum of children with suspected congenital toxoplasmosis. Methods: We accompanied 47 mothers with acquired toxoplasmosis in pregnancy and their children, between June of 2011 and June of 2014. The IgG-WB was done in house and the test was considered positive if the child had antibodies that recognized at least one band on IgG blots different from the mother's or with greater intensity than the corresponding maternal band, during the first three months of life. Results: 15 children (15.1%) met the criteria for congenital toxoplasmosis and 32 (32.3%) had the diagnosis excluded. The symptoms were observed in 12 (80.0%) children and the most frequent were cerebral calcification in 9 (60.0%), chorioretinitis in 8 (53.3%), and hydrocephalus in 4 (26.6%). IgM antibodies anti-T. gondii detected by chemiluminescence (CL) were found in 6 (40.0%) children and the polymerase chain reaction (PCR) for detection of T. gondii DNA was positive in 5 of 7 performed (71.4%). The sensitivity of IgG-WB was of 60.0% [95% confidence interval (CI) 32.3-83.7%] and specificity 43.7% (95% CI 26.7-62.3%). The sensitivity of IgG-WB increased to 76.0 and 89.1% when associated to the research of IgM anti-T. gondii or PCR, respectively. Conclusions: The IgG-WB showed greater sensitivity than the detection of IgM anti-T. gondii; therefore, it can be used for the diagnosis of congenital toxoplasmosis in association with other congenital infection markers.

Resumo Objetivo: Avaliar o método Western Blotting para detecção de IgG anti-Toxoplasma gondii (T. gondii) (IgG-WB) no soro de crianças com suspeita de toxoplasmose congênita. Métodos: Acompanhamos 47 mães com toxoplasmose adquirida na gravidez e seus filhos, entre junho de 2011 e junho de 2014. O IgG-WB foi feito internamente e o teste foi considerado positivo quando a criança apresentava anticorpos que reconheciam pelo menos uma banda nas manchas de IgG diferente das bandas da mãe ou com maior intensidade do que a banda materna correspondente, durante os primeiros 3 meses de vida. Resultados: Atenderam aos critérios para diagnóstico de toxoplasmose congênita 15 crianças (15,1%) e 32 (32,3%) tiveram o diagnóstico excluído. Os sintomas foram observados em 12 crianças (80%) e os mais frequentes foram calcificação cerebral em nove (60%), coriorretinite em oito (53,3%) e hidrocefalia em quatro (26,6%). Os anticorpos IgM anti-T. gondii detectados por quimiluminescência (QL) foram encontrados em seis crianças (40%) e a reação em cadeia da polimerase (RCP) para detecção do DNA de T. gondii foi positiva em cinco de sete reações (71,4%). A sensibilidade do IgG-WB foi de 60% [intervalo de confiança (IC) de 95%, 32,3 a 83,7%] e a especificidade foi de 43,7% (IC de 95%, 26,7 a 62,3%). A sensibilidade do IgG-WB aumentou para 76 e 89,1% quando relacionada à pesquisa de IgM anti-T. gondii ou à RCP, respectivamente. Conclusões: O IgG-WB mostrou maior sensibilidade do que a detecção de IgM anti-T. gondii; portanto, pode ser usado para o diagnóstico de toxoplasmose congênita em associação com outros marcadores de infecção congênita.
Descritores: Toxoplasma/imunologia
Imunoglobulina G/análise
Anticorpos Antiprotozoários/análise
Toxoplasmose Congênita/diagnóstico
Western Blotting/métodos
-Toxoplasma/isolamento & purificação
Toxoplasmose Congênita/imunologia
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
Diagnóstico Precoce
Medições Luminescentes/métodos
Mães
Limites: Humanos
Animais
Feminino
Recém-Nascido
Lactente
Ratos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-1095988
Autor: Antonello, A. M; Pivoto, F. L; Camillo, G; Braunig, P; Sangioni, L. A; Pompermayer, E; Venturini, M. C; Vogel, F. S. F.
Título: Investigação de anticorpos contra Sarcocystis neurona e Sarcocystis cruzi em equinos / Investigation of antibodies against Sarcocystis neurona and Sarcocystis cruzi in equines
Fonte: Arq. bras. med. vet. zootec;67(5):1465-1468tab.
Idioma: pt.
Resumo: Sarcocystis neurona is the primary agent for Equine Protozoal Myeloencephalitis (EPM), important neurological disease characterized by behavior or muscular changes, that impairs animal performance and husbandry. Sarcocystis cruzi is a pathogen related to myositis in cattle. Although related the life cycles of the parasites are distinct. S. neurona has opossums (Didelphis spp.) and S. cruzi, dogs as definitive hosts. However, S. neurona and S. cruzi may undergo cross-reactivity in serological tests, interfering on results of EPM ante-mortem diagnostic tests. In the present study, serology of 189 mares was performed by indirect immunofluorescence antibody test, using antigens of S. neurona and S. cruzi in order to assess the exposure degree of animals to antigens. Analyzing the results, it was observed that most of the animals (84.13%) reacted with at least one protozoal species and the number of animals which showed antibodies against S. cruzi was greater than S. neurona (80.42% and 33.86%, respectively) and a third of seropositive animals reacted to antigens of both species.(AU)
Descritores: Anticorpos Antiprotozoários/análise
Apicomplexa
Sarcocystis/isolamento & purificação
Sarcocistose/veterinária
Sarcocistose/epidemiologia
Encefalomielite/veterinária
Cavalos
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-623639
Autor: Sacks, David L.
Título: B cell dependent T lymphocyte responses in leishmaniasis
Fonte: Mem. Inst. Oswaldo Cruz;83(supl.1):506-513, Nov. 1988.
Idioma: en.
Conferência: Apresentado em: Annual Meeting on Basic Research in Chagas's disease, 15, Apresentado em: Meeting of the Brazilian Society of Protozoology4, Caxambu, 7-10 Nov. 1988.
Resumo: The activation of B cell dependent T cells during Leishmania infection cannot be considered a trivial event, because their removal profoundly alters the course and outcome of infection within genetically susceptible and resistant mouse strains. The demonstration that idiotype recognizing T cells also appear within human populations sensitized to leishmanial antigens as a result of asymptomatic or subclinical infections supports a role for these cells in immunity. These cells are not demonstrable in patients with active visceral disease, so that their role in promoting specific unresponsiveness has not been extended to humans. Whether B cell dependent, idiotype specific T cells represent a functionally distinct T lymphocyte subset with unique regulatory activities remains to be determined.
Descritores: Anticorpos Antiprotozoários/imunologia
Linfócitos B/fisiologia
Leishmania/imunologia
-Linfócitos T
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C3H
Limites: Animais
Cobaias
Camundongos
Responsável: BR1.1 - BIREME


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Id: lil-623605
Autor: Rowland, Edwin C; Spears, Kelly R; McCormick, Thomas S.
Título: Relationship of parasite specific antibodies and heart specific autoantibodies in mice infected with Trypanosoma cruzi
Fonte: Mem. Inst. Oswaldo Cruz;83(supl.1):367-367, Nov. 1988.
Idioma: en.
Conferência: Apresentado em: Annual Meeting on Basic Research in Chagas's disease, 15, Apresentado em: Meeting of the Brazilian Society of Protozoology4, Caxambu, 7-10 Nov. 1988.
Descritores: Autoanticorpos/análise
Anticorpos Antiprotozoários/análise
Doença de Chagas/imunologia
-Trypanosoma cruzi/imunologia
Camundongos Endogâmicos C57BL
Miocárdio/imunologia
Limites: Animais
Cobaias
Camundongos
Responsável: BR1.1 - BIREME


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Id: lil-623600
Autor: Frasch, Alberto C. C; Affranchino, Jose L; Ibañez, Carlos F; Reyes, Maria B; Macina, Roberto A; Luquetti, Alejandro O; Rassi, Anis; Aslund, Lena; Pettersson, Ulf.
Título: Analysis of cloned Trypanosoma cruzi proteins that are antigenic during the acute and chronic phase of Chagas' disease
Fonte: Mem. Inst. Oswaldo Cruz;83(supl.1):345-346, Nov. 1988.
Idioma: en.
Conferência: Apresentado em: Annual Meeting on Basic Research in Chagas's disease, 15, Apresentado em: Meeting of the Brazilian Society of Protozoology4, Caxambu, 7-10 Nov. 1988.
Descritores: Trypanosoma cruzi
Anticorpos Antiprotozoários/análise
Antígenos de Protozoários/genética
-Clonagem Molecular
Doença de Chagas/imunologia
Responsável: BR1.1 - BIREME


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Id: biblio-984760
Autor: Santos, Anna Raquel Ribeiro dos; Serufo, Ângela Vieira; Figueiredo, Maria Marta; Godoi, Lara Carvalho; Vitório, Jéssica Gardone; Marcelino, Andreza Pain; Avelar, Daniel Moreira de; Rodrigues, Fernandes Tenório Gomes; Machado-Coelho, George Luiz Lins; Medeiros, Fernanda Alvarenga Cardoso; Jerônimo, Selma Maria Bezerra; Oliveira, Edward José de; Nascimento, Frederico Crepaldi; Teixeira, Santuza Maria Ribeiro; Gazzinelli, Ricardo Tostes; Nagem, Ronaldo Alves Pinto; Fernandes, Ana Paula.
Título: Evaluation of three recombinant proteins for the development of ELISA and immunochromatographic tests for visceral leishmaniasis serodiagnosis
Fonte: Mem. Inst. Oswaldo Cruz;114:e180405, 2019. tab, graf.
Idioma: en.
Projeto: INCTv; . CNPq; . FAPEMIG; . CNPq; . FAPEMIG; . CAPES; . ARRS.
Resumo: BACKGROUND Visceral Leishmaniasis (VL) is an infectious disease that is a significant cause of death among infants aged under 1 year and the elderly in Brazil. Serodiagnosis is a mainstay of VL elimination programs; however, it has significant limitations due to low accuracy. OBJECTIVE This study aimed to evaluate three recombinant Leishmania infantum proteins (rFc, rC9, and rA2) selected from previous proteomics and genomics analyses to develop enzyme-linked immunosorbent assay (ELISA) and immunochromatographic tests (ICT) for the serodiagnosis of human VL (HVL) and canine VL (CVL). METHODS A total of 186 human (70 L. infantum-infected symptomatic, 20 other disease-infected, and 96 healthy) and 185 canine (82 L. infantum-infected symptomatic, 27 L. infantum-infected asymptomatic, and 76 healthy) sera samples were used for antibody detection. FINDINGS Of the three proteins, rA2 (91.5% sensitivity and 87% specificity) and rC9 (95.7% sensitivity and 87.5% specificity) displayed the best performance in ELISA-HVL and ELISA-CVL, respectively. ICT-rA2 also displayed the best performance for HVL diagnosis (92.3% sensitivity and 88.0% specificity) and had high concordance with immunofluorescence antibody tests (IFAT), ELISA-rK39, IT-LEISH®, and ELISAEXT. ICT-rFc, ICT-rC9, and ICT-rA2 had sensitivities of 88.6%, 86.5%, and 87.0%, respectively, with specificity values of 84.0%, 92.0%, and 100%, respectively for CVL diagnosis. MAIN CONCLUSIONS The three antigens selected by us are promising candidates for VL diagnosis regardless of the test format, although the antigen combinations and test parameters may warrant further optimisation.
Descritores: Ensaio de Imunoadsorção Enzimática
Anticorpos Antiprotozoários/sangue
Leishmania infantum/imunologia
-Cromatografia de Afinidade
Limites: Animais
Cães
Responsável: BR1.1 - BIREME


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Labruna, Marcelo Bahia
Aguiar, Daniel Moura de
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Id: biblio-911890
Autor: Melo, Andréia Lima Tomé; Costa, Andréa Pereira da; Miyazaki, Selma Samiko; Stocco, Matias Bassinello; Semedo, Thiago Borges Fernandes; Pacheco, Thábata dos Anjos; Witter, Rute; Pacheco, Richard de Campos; Labruna, Marcelo Bahia; Marcili, Arlei; Aguiar, Daniel Moura de.
Título: Rural area of the Brazilian Pantanal wetlands associated with the occurrence of anti-Leishmania spp. antibodies in dogs / Área rural do Pantanal brasileiro associada à ocorrência de anticorpos anti-Leishmania spp. em cães
Fonte: Braz. J. Vet. Res. Anim. Sci. (Online);54(4):375-382, 2017. tab, mapas.
Idioma: en.
Projeto: CAPES; . FAPEMAT; . CNPq.
Resumo: An evaluation was made of the presence of anti-Leishmania infantum chagasi antibodies in domestic dogs from the urban and rural areas of Brazil's Pantanal wetland region using serological techniques. A total of 429 dogs were sampled in three areas of the Pantanal biome, including the municipalities of Poconé, Santo Antônio de Leverger, and Barão de Melgaço, in the state of Mato Grosso, and in the municipality of Corumbá, in the state of Mato Grosso do Sul. The immunofluorescence assay (IFA) was used to detect antibodies (cut-off point 40) using Leishmania infantum chagasi antigen. Because of the possibility of cross-reactivity between species of the genus Leishmania, samples that were positive in the IFA against L. infantum chagasi were also tested by IFA in the same conditions, using L. amazonensis and L. braziliensis antigens. IFA-positive samples to L. infantum chagasi were also evaluated using Enzyme-Linked Immunosorbent Assay (ELISA). The results showed the presence of antibodies against L. infantum chagasi in 23 (5.36%; 95% CI: 3.50%-8.05%) dogs and at least one seroreactive dog was found in each of the municipalities evaluated in this study. Antibody titers ranged from 40 to 5,120, and all IFA positive samples were positive in the ELISA. Among the 23 positive dogs, nine were also were seroreactive for L. amazonensis and L. braziliensis antigens. The occurrence of anti- L. infantum chagasi antibodies in dogs was higher in rural areas (7.06%) than in urban areas (2.50%) (P < 0.05). Based on this study, we concluded that dogs from rural areas of the Pantanal wetlands were in contact with Leishmania species, which is relevant information given their importance to public health.(AU)

Neste trabalho foi realizada uma avaliação sobre a presença de anticorpos anti-Leishmania infantum chagasi em cães domésticos das áreas urbanas e rurais da região do Pantanal brasileiro usando técnicas sorológicas. Um total de 429 cães foram amostrados em três áreas do bioma do Pantanal, incluindo os municípios de Poconé, Santo Antônio de Leverger e Barão de Melgaço, em Mato Grosso, e o município de Corumbá, em Mato Grosso do Sul. A reação de imunofluorescência indireta (RIFI) foi utilizada para detectar anticorpos (ponto de corte de 40) de Leishmania infantum chagasi como antígeno. Devido à possibilidade de reação cruzada entre as espécies do gênero Leishmania, as amostras positivas na RIFI para L. infantum chagasi foram também avaliadas na RIFI utilizando L. amazonensis e L. braziliensis como antígenos. As amostras positivas na RIFI para L. infantum chagasi foram avaliadas utilizando o ensaio de imunoadsorção ligado à enzima (ELISA). Os resultados mostraram a presença de anticorpos contra L. infantum chagasi em 23 (5,36%; IC 95%: 3,50% -8,05%) cães e pelo menos um cão soro-reativo foi encontrado em todos os municípios avaliados neste estudo. Os títulos de anticorpos variaram de 40 a 5.120 e todas as amostras positivas na RIFI foram positivas no ELISA. Entre os 23 cães positivos, nove também reagiram para L. amazonensis e L. braziliensis. A ocorrência de anticorpos anti-L. infantum chagasi em cães foi maior nas áreas rurais (7,06%) do que nas áreas urbanas (2,50%) (P < 0,05). Com base neste estudo, concluímos que cães de áreas rurais do Pantanal tiveram contato com espécies de Leishmania, o que é uma informação relevante, dada a sua importância para a saúde pública.(AU)
Descritores: Anticorpos Antiprotozoários/análise
Leishmania infantum/imunologia
-Brasil/epidemiologia
Ensaio de Imunoadsorção Enzimática/veterinária
Técnica Indireta de Fluorescência para Anticorpo/veterinária
Limites: Animais
Cães
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-894905
Autor: Aguilera, Elena; Varela, Javier; Serna, Elva; Torres, Susana; Yaluff, Gloria; Bilbao, Ninfa Vera de; Cerecetto, Hugo; Alvarez, Guzmán; González, Mercedes.
Título: Looking for combination of benznidazole and Trypanosoma cruzi-triosephosphate isomerase inhibitors for Chagas disease treatment
Fonte: Mem. Inst. Oswaldo Cruz;113(3):153-160, Mar. 2018. graf.
Idioma: en.
Projeto: CSIC; . Agencia Nacional de Investigación e Innovación.
Resumo: BACKGROUND The current chemotherapy for Chagas disease is based on monopharmacology with low efficacy and drug tolerance. Polypharmacology is one of the strategies to overcome these limitations. OBJECTIVES Study the anti-Trypanosoma cruzi activity of associations of benznidazole (Bnz) with three new synthetic T. cruzi-triosephosphate isomerase inhibitors, 2, 3, and 4, in order to potentiate their actions. METHODS The in vitro effect of the drug combinations were determined constructing the corresponding isobolograms. In vivo activities were assessed using an acute murine model of Chagas disease evaluating parasitaemias, mortalities and IgG anti-T. cruzi antibodies. FINDINGS The effect of Bnz combined with each of these compounds, on the growth of epimastigotes, indicated an additive action or a synergic action, when combining it with 2 or 3, respectively, and an antagonic action when combining it with 4. In vivo studies, for the two chosen combinations, 2 or 3 plus one fifth equivalent of Bnz, showed that Bnz can also potentiate the in vivo therapeutic effects. For both combinations a decrease in the number of trypomastigote and lower levels of anti-T. cruzi IgG-antibodies were detected, as well clear protection against death. MAIN CONCLUSIONS These results suggest the studied combinations could be used in the treatment of Chagas disease.
Descritores: Triose-Fosfato Isomerase/química
Trypanosoma cruzi/efeitos dos fármacos
Trypanosoma cruzi/imunologia
Nitroimidazóis/farmacologia
-Anticorpos Antiprotozoários
Sinergismo Farmacológico
Quimioterapia Combinada
Responsável: BR1.1 - BIREME


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Id: biblio-841805
Autor: Baschirotto, Priscila T; Krieger, Marco A; Foti, Leonardo.
Título: Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
Fonte: Mem. Inst. Oswaldo Cruz;112(6):428-436, June 2017. tab, graf.
Idioma: en.
Resumo: BACKGROUND During pregnancy, toxoplasmosis and rubella can cause serious damage to the mother and the foetus through vertical transmission. Early diagnosis enables implementation of health measures aimed at preventing vertical transmission and minimising damage caused by these diseases. OBJECTIVE Here, we report the development of a multiplex assay for simultaneous detection of IgG antibodies produced during toxoplasmosis and rubella infection. METHODS This assay is based on xMap technology. Initially, by singleplex assays, we evaluated the following antigens: one Toxoplasma gondii lysate; two antigenic extracts of T. gondii (TOX8131 and TOX8122); fragments of T. gondii antigens [SAG-1 (amino acids 45-198), GRA-7 (24-100), GRA-1 (57-149), ROP-4, and MIC-3 (234-306)]; two chimeric antigens composed of fragments of SAG-1, GRA-7, and P35 (CTOX and CTOXH); and fragments of Rubella virus antigens [E-1 (157-176, 213-239, 374-390), E-2 (31-105), and C (1-123)]. FINDINGS A multiplex assay to simultaneously diagnose toxoplasmosis and rubella was designed with the best-performing antigens in singleplex and multiplex assays, which included CTOXH, T. gondii lysate, TOX8131, E-1, and E-2. The multiplex assay showed 100% sensitivity and specificity for anti-T. gondii IgG detection and 95.6% sensitivity and 100% specificity for anti-R. virus IgG detection. MAIN CONCLUSIONS We found that, despite the difficulties related to developing multiplex systems, different types of antigens (extracts and recombinant proteins) can be used to develop high-performance diagnostic tests. The assay developed is suitable to screen for prior T. gondii and R. virus infections, because it is a rapid, high-throughput, low-cost alternative to the current standard diagnostic tools, which require multiple individual tests.
Descritores: Vírus da Rubéola/imunologia
Toxoplasma/imunologia
Anticorpos Antiprotozoários/sangue
Toxoplasmose/imunologia
Anticorpos Antivirais/sangue
-Rubéola (Sarampo Alemão)/diagnóstico
Imunoensaio
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase Multiplex
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-719505
Autor: Minaya-Gómez, Gloria; Vargas-Apaza, Silver; Monteza-Zuloeta, Yolanda; Purisaca-Morante, Enrique; Delgado-Diaz, Freddy.
Título: Especificidad de la prueba intradérmica de Montenegro en pacientes infectados por Trypanosoma cruzi procedentes de diferentes regiones del Perú / Specificity of the intradermal Montenegro test in patients infected by Trypanosoma cruzi from different regions of Peru
Fonte: Rev. peru. med. exp. salud publica;31(2):278-281, abr.-jun. 2014. tab.
Idioma: es.
Resumo: Con el objetivo de evaluar la especificidad de la leishmanina en pacientes con enfermedad de Chagas, sin antecedente clínico de leishmaniosis, actual o antigua. Una muestra de 102 personas infectadas por Trypanosoma cruzi (14 casos agudos con diagnóstico parasitológico y 88 casos crónicos a través de la demostración de anticuerpos IgG por ELISA e inmunofluorescencia indirecta (IFI) fueron evaluados con leishmanina, antígeno soluble que contenia Leishmania (Viannia) peruviana a la concentración de 25-30 ug/mL. Solo cinco personas presentaron reacción de hipersensibilidad cutánea a la aplicación del antígeno a las 48-72 h. La leishmanina evaluada fue negativa en 97 personas infectadas con T. cruzi, con lo cual se alcanzó una especificidad del 95,1%. En conclusión, la prueba intradérmica de Montenegro es una herramienta diagnóstica simple y eficaz, que además podría ser utilizada para discriminar infecciones por Leishmania o T. cruzi, en áreas geográficas del Perú donde ambos parásitos están presentes.

In order to assess the specificity of the leishmanin skin test in Chagas disease patients without clinical history of leishmaniasis, present or former. A sample of 102 persons infected with Trypanosoma cruzi (14 acute cases with parasitological diagnosis and 88 chronic cases) through the demonstration of IgG antibodies by ELISA and indirect immunofluorescence (IIF) were evaluated with leishmanin soluble antigen which contained Leishmania (Viannia) peruviana concentration of 25-30 ug/mL. Only five people showed cutaneous hypersensitivity reaction to the application of the antigen between hours 48 and 72. The Leishmanin skin test evaluated was negative in 97 people infected with T. cruzi, thus specificity of 95.1% was achieved. In conclusion, the intradermal Montenegro test is a simple and effective diagnostic tool that also could be used to discriminate infections by Leishmania or T. cruzi, in Peruvian geographic areas where both parasites are present.
Descritores: Doença de Chagas/diagnóstico
-Anticorpos Antiprotozoários/análise
Doença de Chagas/imunologia
Peru
Sensibilidade e Especificidade
Testes Cutâneos/métodos
Trypanosoma cruzi/imunologia
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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