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Id: biblio-1022226
Autor: Hyde, T B; Sato, H K; Hao, L; Flannery, B; Zhenq, Q; Wannemuehler, K; Ciccone, F H; Marques, H Sousa de; Weckx, L Y; Sáfadi, M A; Moraes, E Oliveira de; Pinhata, M M; Olbrich Neto, J; Bevilacqua, M C; Tabith Junior, A; Monteiro, T A; Figueiredo, C A; Andrus, J K; Reef, S E; Toscano, C M; Castilho-Solorzano, C; Icenogle, J P.
Título: Identification of serologic markers for school-aged children with congenital rubella syndrone
Fonte: J Infect Dis;212(1):57-66, 2014.
Idioma: en.
Resumo: BACKGROUND: Congenital rubella syndrome (CRS) case identification is challenging in older children since laboratory markers of congenital rubella virus (RUBV) infection do not persist beyond age 12 months. METHODS: We enrolled children with CRS born between 1998 and 2003 and compared their immune responses to RUBV with those of their mothers and a group of similarly aged children without CRS. Demographic data and sera were collected. Sera were tested for anti-RUBV immunoglobulin G (IgG), IgG avidity, and IgG response to the 3 viral structural proteins (E1, E2, and C), reflected by immunoblot fluorescent signals. RESULTS: We enrolled 32 children with CRS, 31 mothers, and 62 children without CRS. The immunoblot signal strength to C and the ratio of the C signal to the RUBV-specific IgG concentration were higher (P < .029 for both) and the ratio of the E1 signal to the RUBV-specific IgG concentration lower (P = .001) in children with CRS, compared with their mothers. Compared with children without CRS, children with CRS had more RUBV-specific IgG (P < .001), a stronger C signal (P < .001), and a stronger E2 signal (P ≤ .001). Two classification rules for children with versus children without CRS gave 100% specificity with >65% sensitivity. CONCLUSIONS: This study was the first to establish classification rules for identifying CRS in school-aged children, using laboratory biomarkers. These biomarkers should allow improved burden of disease estimates and monitoring of CRS control programs. Published by Oxford University Press on behalf of the Infectious Diseases Society of America 2014. This work is written by (a) US Government employee(s) and is in the public domain in the US.
Descritores: Instituições Acadêmicas
Estudantes
Síndrome da Rubéola Congênita/diagnóstico
Biomarcadores/sangue
Adolescente
Anticorpos Antivirais
Afinidade de Anticorpos
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-1022067
Autor: Bisordi, I; Levis, S; Maeda, A Y; Suzuki, A; Nagasse-Sugahara, T K; Souza, R P; Pereira, L E; Garcia, J B; Cerroni, M de P; Silva, F de A; Santos, C L dos; Fonseca, B A da.
Título: Pinhal virus, a new arenavirus isolated from calomys tener in Brazil
Fonte: Vector Borne Zoonotic Dis;15(11):694-700, 2015.
Idioma: en.
Resumo: Arenavirus Sabiá was originally isolated from a fatal human infection in Brazil, and after the occurrence of the second fatal human case in São Paulo state, epidemiologic and virologic studies were performed in the area where the patient lived, aiming at the identification of the Sabiá natural rodent reservoir. A broadly cross-reactive enzyme-linked immunosorbent assay (ELISA) was used to screen for antibody-positive samples. Antibodies to arenavirus were detected in two of the 55 samples of Calomys tener, and from these results, samples of rodents were analyzed by a broad RT-PCR assay. RT-PCR amplification detected arenavirus sequences in five of the 55 C. tener samples, and sequencing showed that this virus is a distinct form of Sabiá virus. Thus, we describe here the evidence for the circulation of a new arenavirus in Brazil (proposed name Pinhal virus) and its genetic characterization compared to other arenaviruses. This study also suggests C. tener as a probable rodent reservoir for this virus and associates this new virus with the lineage C of New World arenaviruses. Although we have defined some characteristics of this virus, so far, there is no evidence of its involvement in human disease.
Descritores: Filogenia
Brasil/epidemiologia
Reservatórios de Doenças/veterinária
Ensaio de Imunoadsorção Enzimática
Sigmodontinae/virologia
Arenavirus do Novo Mundo/isolamento & purificação
Arenavirus do Novo Mundo/classificação
Arenavirus do Novo Mundo/genética
Infecções por Arenaviridae/veterinária
Infecções por Arenaviridae/virologia
Animais
Anticorpos Antivirais/sangue
Responsável: BR91.2 - Centro de Documentação


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Texto completo SciELO Brasil
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Id: biblio-841817
Autor: Requena-Castro, Rocío; Reyes-López, Miguel Ángel; Rodríguez-Reyna, Rosa Eminé; Palma-Nicolás, Prisco; Bocanegra-García, Virgilio.
Título: Molecular detection of mixed infections with multiple dengue virus serotypes in suspected dengue samples in Tamaulipas, Mexico
Fonte: Mem. Inst. Oswaldo Cruz;112(7):520-522, July 2017. tab, graf.
Idioma: en.
Projeto: CONACYT.
Resumo: This study aimed to detect dengue virus (DENV) serotypes in serum samples obtained in Matamoros Tamaulipas, Mexico, and to determine the concordance of conventional nested reverse transcriptase polymerase chain reaction (RT-PCR) and a serological test [enzyme-linked immunosorbent assay (ELISA NS1)]. Here, we detected mixed infections consisting of four serotypes of DENV. The most prevalent serotype was DENV-1, followed by DENV-4. This is the first report of DENV-4 in our region. Mixed infections were also detected in 21.5% of samples, and the predominant coinfection consisted of DENV-1 and DENV-2. Therefore, continuous epidemiological surveillance of DENV in this area is required to predict future forms of dengue heterologous infections and the effect of this on health care.
Descritores: Ensaio de Imunoadsorção Enzimática
Reação em Cadeia da Polimerase
DNA Polimerase Dirigida por RNA
Dengue/diagnóstico
Dengue/virologia
Vírus da Dengue/genética
Sorogrupo
Anticorpos Antivirais/sangue
-México
Limites: Seres Humanos
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-841805
Autor: Baschirotto, Priscila T; Krieger, Marco A; Foti, Leonardo.
Título: Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella
Fonte: Mem. Inst. Oswaldo Cruz;112(6):428-436, June 2017. tab, graf.
Idioma: en.
Resumo: BACKGROUND During pregnancy, toxoplasmosis and rubella can cause serious damage to the mother and the foetus through vertical transmission. Early diagnosis enables implementation of health measures aimed at preventing vertical transmission and minimising damage caused by these diseases. OBJECTIVE Here, we report the development of a multiplex assay for simultaneous detection of IgG antibodies produced during toxoplasmosis and rubella infection. METHODS This assay is based on xMap technology. Initially, by singleplex assays, we evaluated the following antigens: one Toxoplasma gondii lysate; two antigenic extracts of T. gondii (TOX8131 and TOX8122); fragments of T. gondii antigens [SAG-1 (amino acids 45-198), GRA-7 (24-100), GRA-1 (57-149), ROP-4, and MIC-3 (234-306)]; two chimeric antigens composed of fragments of SAG-1, GRA-7, and P35 (CTOX and CTOXH); and fragments of Rubella virus antigens [E-1 (157-176, 213-239, 374-390), E-2 (31-105), and C (1-123)]. FINDINGS A multiplex assay to simultaneously diagnose toxoplasmosis and rubella was designed with the best-performing antigens in singleplex and multiplex assays, which included CTOXH, T. gondii lysate, TOX8131, E-1, and E-2. The multiplex assay showed 100% sensitivity and specificity for anti-T. gondii IgG detection and 95.6% sensitivity and 100% specificity for anti-R. virus IgG detection. MAIN CONCLUSIONS We found that, despite the difficulties related to developing multiplex systems, different types of antigens (extracts and recombinant proteins) can be used to develop high-performance diagnostic tests. The assay developed is suitable to screen for prior T. gondii and R. virus infections, because it is a rapid, high-throughput, low-cost alternative to the current standard diagnostic tools, which require multiple individual tests.
Descritores: Vírus da Rubéola/imunologia
Toxoplasma/imunologia
Anticorpos Antiprotozoários/sangue
Toxoplasmose/imunologia
Anticorpos Antivirais/sangue
-Rubéola (Sarampo Alemão)/diagnóstico
Imunoensaio
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase Multiplex
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Romero, Gustavo Adolfo Sierra
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Id: biblio-955113
Autor: Prado, Paulo Sousa; Almeida Júnior, José Teófilo Duarte; Abreu, Lanna Takada de; Silva, Cristina Gabriel; Souza, Larissa da Costa; Gomes, Marizoneide Cavalcante; Mendes, Lucinda Malheiros Teixeira; Santos, Eliane Maria dos; Romero, Gustavo Adolfo Sierra.
Título: Validation and reliability of the rapid diagnostic test 'SD Bioeasy Dengue Duo' for dengue diagnosis in Brazil: a phase III study
Fonte: Mem. Inst. Oswaldo Cruz;113(8):e170433, 2018. tab, graf.
Idioma: en.
Resumo: BACKGROUND The diagnosis of dengue is complex. Until recently, only specialised laboratories were able to confirm dengue infection. However, this has changed with the newly available immunochromatographic rapid tests. Early diagnosis is of great interest, and point-of-care rapid tests have been increasingly used in Brazil. Most of those tests have not undergone validation in the Brazilian population. In this context, we decided to evaluate a rapid test introduced in the Federal District (FD). OBJECTIVES To estimate the accuracy and reliability of the SD Bioeasy Dengue Duo rapid test and its components to detect dengue infections in a consecutive sample of symptomatic residents in the FD, Brazil. METHODS In total, 1353 venous blood samples were collected between 2013 and 2014. Two hundred and six positive samples (cases) and 246 negative samples (non cases) were required for sensitivity and specificity estimation, respectively; for agreement evaluation, we used 401 samples. The reference standard used was a composite of MAC-ELISA, virus isolation and real-time polymerase chain reaction (RT-qPCR). The evaluation was conducted prospectively under field conditions in the public health units of the FD. FINDINGS The results for the overall accuracy of the rapid test (NS1/IgM combined) showed 76% sensitivity and 98% specificity. The sensitivity for the NS1 component (67%) was better than that for the IgM component (35%). The positive likelihood ratio was 46, and the negative likelihood ratio was 0.24. The reliability of the test (NS1/IgM combined) demonstrated crude agreement of 98% (Kappa index 0.94). MAIN CONCLUSIONS The present phase III, large-scale validation study demonstrates that the rapid test SD Bioeasy Dengue Duo has moderate sensitivity (NS1/IgM combined) and high specificity. Therefore, the test is useful in confirming the diagnosis of dengue, but not enough to rule out the diagnosis. Our results also suggest that Dengue virus (DENV) viral load estimated through the RT-qPCR and antibody level measured through the MAC-ELISA could have had a direct influence on the accuracy of the rapid test.
Descritores: Imunoglobulina M/análise
Dengue/diagnóstico
Diagnóstico Precoce
-Brasil/epidemiologia
Chile/epidemiologia
Reação em Cadeia da Polimerase em Tempo Real
Anticorpos Antivirais/sangue
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Saúde Pública
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Id: biblio-961709
Autor: Ministerio del Poder Ciudadano para la Salud de Nicaragua.
Título: Seroprevalencia y tasa de ataque clínica por chikungunya en Nicaragua, 2014-2015 / Chikungunya seroprevalence and clinical case rate in Nicaragua, 2014-2015
Fonte: Rev. panam. salud pública = Pan am. j. public health;41:e59, 2017. tab, graf.
Idioma: es.
Resumo: RESUMEN Objetivo Estimar la seroprevalencia, la tasa de ataque clínica y la proporción de infecciones subclínicas por chikungunya, Métodos Se realizó un estudio transversal en 39 sitios distribuidos en todo el territorio nacional de Nicaragua en octubre 2015. Se recopiló información demográfica y clínica a través de una encuesta personal. Se recolectaron muestras hemáticas para detectar la presencia de anticuerpos antivirus chikungunya utilizando el método de ELISA de inhibición desarrollado por el Centro Nacional de Diagnóstico y Referencia. Se utilizaron modelos lineales generalizados y modelos de multinivel de Poisson en el análisis de los resultados. Resultados Se enrolaron 11 722 participantes mayores de dos años de edad y se procesaron 11 280 muestras. En el nivel nacional, la seroprevalencia fue de 32,8% (IC95% [intervalo de confianza de 95%]: 31,9-33,6), con una tasa de ataque clínica de 26,5% (IC95%: 25,7-27,3) y una proporción de infecciones subclínicas de 19,1% (IC95%: 17,8-20,4). Se observó variabilidad en la seroprevalencia de los 39 sitios, y los que presentaron mayor índice de infestación por el vector mostraron una mayor seroprevalencia. A nivel individual, esta fue más elevada en los participantes mayores de 11 años. Conclusión Este es el primer estudio sobre la seroprevalencia de chikungunya en América Latina continental desde su introducción, en el que se determinaron la prevalencia a nivel nacional, la tasa de ataque clínico y la proporción de infecciones subclínicas. El modelo utilizado, con una amplia participación comunitaria y el rol rector del Ministerio de Salud de Nicaragua, puede constituir un ejemplo para la realización de estudios similares en la región.

ABSTRACT Objective Estimate seroprevalence, clinical case rate, and proportion of subclinical infections from chikungunya. Methods A cross-sectional study was conducted in October 2015 at 39 sites distributed across Nicaragua. Demographic and clinical information was compiled through a personal survey. Blood samples were collected to detect chikungunya antibodies using the ELISA inhibition method developed by Nicaragua's National Diagnostic and Reference Center. Results were analyzed using generalized linear models and multilevel Poisson models. Results A total of 11 722 participants aged >2 years were enrolled and 11 280 samples were processed. National seroprevalence was 32.8% (95% CI [95% confidence interval]: 31.9-33.6), with a clinical case rate of 26.5% (95% CI: 25.7-27.3) and a proportion of subclinical infections of 19.1% (95% CI: 17.8-20.4). Seroprevalence varied among the 39 sites and was greater at sites with higher vector infestation indices. Individually, seroprevalence was higher in participants aged >11 years. Conclusion Since its introduction, this is the first study on chikungunya seroprevalence in continental Latin America to determine national prevalence, clinical case rate, and proportion of subclinical infections. The study model, employing broad community participation and leadership by the Ministry of Health of Nicaragua, can be an example for conducting similar studies in the region.
Descritores: Vírus Chikungunya/imunologia
Febre de Chikungunya/diagnóstico
Febre de Chikungunya/epidemiologia
Anticorpos Antivirais/sangue
-Nicarágua/epidemiologia
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Texto completo SciELO Saúde Pública
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Id: biblio-961706
Autor: Galo, Saira Saborío; González, Karla; Téllez, Yolanda; García, Nadezna; Pérez, Leonel; Gresh, Lionel; Harris, Eva; Balmaseda, Ángel.
Título: Development of in-house serological methods for diagnosis and surveillance of chikungunya / Desarrollo de métodos serológicos propios para el diagnóstico y la vigilancia del chikungunya
Fonte: Rev. panam. salud pública = Pan am. j. public health;41:e56, 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective To develop and evaluate serological methods for chikungunya diagnosis and research in Nicaragua. Methods Two IgM ELISA capture systems (MAC-ELISA) for diagnosis of acute chikungunya virus (CHIKV) infections, and two Inhibition ELISA Methods (IEM) to measure total antibodies against CHIKV were developed using monoclonal antibodies (mAbs) and hyperimmune serum at the National Virology Laboratory of Nicaragua in 2014-2015. The sensitivity, specificity, predictive values, and agreement of the MAC-ELISAs were obtained by comparing the results of 198 samples (116 positive; 82 negative) with the Centers for Disease Control and Prevention's IgM ELISA (Atlanta, Georgia, United States; CDC-MAC-ELISA). For clinical evaluation of the four serological techniques, 260 paired acute and convalescent phase serum samples of suspected chikungunya cases were used. Results All four assays were standardized by determining the optimal concentrations of the different reagents. Processing times were substantially reduced compared to the CDC-MAC-ELISA. For the MAC-ELISA systems, a sensitivity of 96.6% and 97.4%, and a specificity of 98.8% and 91.5% were obtained using mAb and hyperimmune serum, respectively, compared with the CDC method. Clinical evaluation of the four serological techniques versus the CDC real-time RT-PCR assay resulted in a sensitivity of 95.7% and a specificity of 88.8%-95.9%. Conclusion Two MAC-ELISA and two IEM systems were standardized, demonstrating very good quality for chikungunya diagnosis and research demands. This will achieve more efficient epidemiological surveillance in Nicaragua, the first country in Central America to produce its own reagents for serological diagnosis of CHIKV. The methods evaluated here can be applied in other countries and will contribute to sustainable diagnostic systems to combat the disease.

RESUMEN Objetivo Elaborar y evaluar métodos serológicos para el diagnóstico y la investigación del chikungunya. Métodos Se elaboraron dos sistemas de ELISA de captura de IgM (MAC-ELISA por sus siglas en inglés) para el diagnóstico de la infección aguda por el virus de (CHIKV) y dos métodos de ELISA de inhibición (MEI) para determinar el valor cuantitativo de los anticuerpos totales contra el CHIKV, en el Laboratorio Nacional de Virología de Nicaragua en 2014-2015, para lo cual se utilizaron anticuerpos monoclonales (AcMo) y sueros hiperinmunes. Se determinó la sensibilidad, la especificidad y los valores predictivos, así como la concordancia de los MAC-ELISA, comparando los resultados de 198 muestras (116 positivas y 82 negativas) con el ELISA de los Centros para el Control y la Prevención de Enfermedades de los Estados Unidos (Atlanta; MAC-ELISA-CDC). Para la evaluación clínica de las cuatro técnicas serológicas, se emplearon 260 muestras de suero obtenidas en la fase aguda y en la fase de convalecencia de presuntos casos de chikungunya. Resultados Se estandarizaron los cuatro métodos analíticos determinando las concentraciones óptimas de los diferentes reactivos. La duración del procesamiento se redujo sustancialmente en comparación con el MAC-ELISA-CDC. Con los sistemas de MAC-ELISA, se obtuvo una sensibilidad del 96,6% y del 97,4% y una especificidad del 98,8% y del 91,5% al utilizar AcMo y suero hiperinmune, respectivamente, en comparación con el método de los CDC. La evaluación clínica de las cuatro técnicas serológicas, en comparación con la PCR en tiempo real de los CDC, arrojó una sensibilidad del 95,7% y una especificidad del 88,8%-95,9%. Conclusiones Se estandarizaron dos sistemas de ELISA-MAC y dos de MEI y se comprobó que poseen la calidad adecuada para el diagnóstico y las investigaciones del chikungunya, con lo cual mejorará la eficiencia de la vigilancia epidemiológica en Nicaragua, el primer país centroamericano que produce sus propios reactivos para el diagnóstico serológico del CHIKV. Los métodos estudiados en este trabajo pueden aplicarse en otros países y contribuyen al desarrollo de sistemas de diagnóstico sostenibles para combatir la enfermedad.
Descritores: Vírus Chikungunya/imunologia
Febre de Chikungunya/diagnóstico
Febre de Chikungunya/epidemiologia
Anticorpos Antivirais/sangue
-Ensaio de Imunoadsorção Enzimática/métodos
Nicarágua/epidemiologia
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Texto completo
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Id: biblio-895551
Autor: Morikawa, Vivien M; Zimpel, Cristina K; Paploski, Igor A. D; Kikuti, Mariana; Castro, Vanessa; Lopes, Marcia O; Biondo, Alexander W; Barros Filho, Ivan R. de.
Título: Serological survey of anti-Leptospira spp. antibodies in Barbary sheep (Ammotragus lervia) at the Curitiba Zoo, southern Brazil / Estudo sorológico de anticorpos anti-Leptospira spp. em aoudads (Ammotragus lervia) no zoológico de Curitiba, sul do Brasil
Fonte: Pesqui. vet. bras = Braz. j. vet. res;38(1):143-146, Jan. 2018. tab, ilus.
Idioma: en.
Resumo: Leptospirosis is a worldwide zoonosis, affecting humans, domestic and wild animals. The present study aimed to evaluate prevalence of anti-Leptospira spp. antibodies in Barbary sheep at the Curitiba zoo. Microscopic agglutination test (MAT) was performed using 17 serogroups. Antibodies against Leptospira spp. were observed in 23.5% samples and Icterohaemorrhagiae was the only prevalent serogroup. The presence of anti-Leptospira antibodies in Barbary sheep indicates exposure to leptospires; thus monitoring and preventive measures are necessary in zoo's captive animals, since they can act as sentinels of environmental exposure in an area with high movement of people.(AU)

A leptospirose é uma zoonose mundial que afeta seres humanos, animais domésticos e selvagens. O presente estudo objetivou avaliar a prevalência de anticorpos anti-Leptospira spp. em aoudads do zoológico de Curitiba. Foi realizado o teste de Soroaglutinação microscópica (SAM) utilizando 17 sorogrupos. Anticorpos contra Leptospira spp. foram observados em 23.5% das amostras de aoudads e Icterohaemorrhagiae foi o único sorogrupo prevalente. A presença de anticorpos em aoudads indica exposição a leptospiras portanto monitoramento e medidas preventivas são necessários em animais confinados em zoológicos, uma vez eles podem atuar como sentinelas de exposição ambiental em uma área com alta circulação de pessoas.(AU)
Descritores: Anticorpos Antivirais/análise
Leptospirose/epidemiologia
Ruminantes/imunologia
-Animais de Zoológico/imunologia
Testes Sorológicos/veterinária
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-870271
Autor: Castanha, Priscila Mayrelle da Silva.
Título: Transferência placentária e cinética de anticorpos antidengue materno transferidos em uma coorte de crianças no primeiro ano de vida / Placental transfer and kineticis of maternal transferred dengue-specific antibodies in a cohort of children during the first year.
Fonte: Recife; s.n; 2016. 97 p. ilus, graf, tab.
Idioma: pt.
Tese: Apresentada a Centro de Pesquisas Aggeu Magalhães para obtenção do grau de Doutor.
Resumo: Anticorpos antidengue materno transferidos têm sido implicados na imunopatogênese da dengue grave em lactentes. Postula-se que esses anticorpos possuam um papel distinto durante os primeiros anos de vida: ao nascimento, anticorpos antidengue materno adquiridos conferem proteção, e em seguida declinam a níveis subneutralizantes capazes de facilitar a infecção pelo vírus dengue (DENV) mediante o mecanismo de ADE (Antibody dependent enhancement), aumentando o risco de ocorrência das formas graves da dengue. Estudos prospectivos conduzidos em lactentes Asiáticos têm mostrado que o pico de anticorpos materno adquiridos com a capacidade de mediar aumento da infecção pelo DENV ocorre entre o sexto e o nono mês de vida, o que correlaciona com a epidemiologia da dengue grave em lactentes dessa região. Esta tese descreve o perfil de imunidade materna antidengue e a transferência placentária de anticorpos dengue-específicos em pares mãe-cordão recrutados em um estudo de coorte prospectivo conduzido na cidade do Recife, Nordeste do Brasil. Adicionalmente, esse trabalho analisa o papel dos níveis de IgG total maternos e da imunidade antidengue materna na transferência de anticorpos ao feto. Na coorte de lactentes, a tese avalia a cinética de declínio dos anticorpos antidengue materno-transferidos e sua capacidade de mediar ADE durante os primeiros anos de vida. Níveis de IgG DENV-especifico e de anticorpos neutralizantes sorotipo específicos (DENV1-4) foram determinados em 376 pares mãe-cordão. A cinética de anticorpos materno transferidos neutralizantes e/ou mediadores de ADE foi investigada em uma subamostra das crianças inseridas na coorte. A maior parte das gestantes apresentava imunidade ao sorotipo DENV-3 (53,7 por cento) ou a combinação DENV-3/ DENV-4 (30,6 por cento)...

Maternal-fetal transferred Dengue virus (DENV)-specific antibodies have been implicated in the immunopathogenesis of dengue during infancy. These antibodies play a dual role in infants during the first year of life: first, maternally-acquired antibodies confer protection at birth, and then decline to a lower level capable of enhance DENV infection through the mechanism of ADE (antibody-dependent enhancement), increasing the chance of development of severe dengue. Prospective studies conducted in Asian infants have provided evidence that the peak of enhancing activity by maternally transferred dengue antibodies occurs between 6th to 9th month of age, which correlates with the age-related epidemiology of the dengue severe cases in this region. This thesis describes the placental transfer of dengue-specific antibodies in mother-cord pairs enrolled in a prospective cohort study carried out in the city of Recife, Northeast Brazil. Moreover, we analyze the role of maternal total IgG levels and dengue immunity in the transference of dengue-specific antibodies to the fetus. In the cohort of children, we determine the kinetics of Enhancing Activity (EA) by maternally acquired dengue antibodies during their first year of life. DENV-specific IgG and serotype-specific (DENV1-4) neutralizing antibody (Nab) levels were assessed in 376 mother-cord paired samples...
Descritores: Dengue/epidemiologia
Imunidade Materno-Adquirida
Lactente
Vírus da Dengue/imunologia
-Anticorpos Antivirais/sangue
Anticorpos Neutralizantes/sangue
Brasil
Estudos de Coortes
Imunoglobulina G/sangue
Limites: Seres Humanos
Responsável: BR305.1 - Biblioteca do CPqAM
BR305.1; (043.2)"2016", C346t


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Id: biblio-846779
Autor: Silva, Elís Rosélia Dutra de Freitas Siqueira; Castro, Vanessa; Prianti, Maria das Graças; Gonçalves, Larissa Maria Feitosa; Sobrinho Júnior, Enoque Pereira Costa; Drumond, Karina Oliveira; Mineiro, Ana Lys Bezerra Barradas.
Título: Occurrence of antibodies against Leptospira spp in dogs from Teresina, Piauí, Brazil / Ocorrência de anticorpos contra Leptospira spp em cães de Teresina, Piauí, Brasil
Fonte: Braz. J. Vet. Res. Anim. Sci. (Online);54(1):88-91, 2017. tab..
Idioma: en.
Resumo: Leptospirosis is a systemic disease caused by the species of bacteria Leptospira spp., which affects human beings, domestic and wild animals. The present study searched the presence of antibodies against Leptospira spp. in the canine population of the city Teresina, Piauí, and the most common serovars. Blood samples from 425 stray dogs were collected in the local zoonosis center in Teresina from July 2010 to January 2012 and submitted to the Microscopic Seroagglutination Test (MAT). This study found an average infection rate of 17.41% (IC 95%; 13,8 ­ 21,0) by 11 different serovars; the four most frequent were Canicola (18.9%), Autumnalis (16.2%), Icterohaemorrhagiae (12.1%), and Butembo (12.1%). The questions raised in this study indicated the occurrence of Leptospira spp infection in dogs of Terezina- Piaui, Brazil.(AU)

A leptospirose é uma doença sistêmica causada por bactéria Leptospira spp. que afeta seres humanos, animais domésticos e selvagens. O presente trabalho investigou a presença de anticorpos anti-Leptospira spp. na população canina da cidade de Teresina-Piauí, e os respectivos sorovares predominantes. Amostras de sangue de 425 cães foram coletadas no Centro de Controle de Zoonoses de Teresina, no período de julho de 2010 a janeiro de 2012, e submetidas à prova de Soroaglutinação Microscópica (SAM). Este estudo encontrou uma taxa de infecção média com 17,41% (IC 95%; 13,8 ­ 21,0) e 11 sorovares reagentes, sendo os prevalentes Canicola (18,9%), Autumnalis (16,2%), Icterohaemorrhagiae (12,1%) e Butembo (12,1%). As questões levantadas neste estudo indicam a ocorrência de infecção por Leptospira spp em cães da cidade de Teresina-Piauí, Brasil.(AU)
Descritores: Anticorpos Antivirais/análise
Leptospira/isolamento & purificação
Leptospirose/epidemiologia
Limites: Animais
Cães
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice



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