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Pesquisa : D12.776.157 [Categoria DeCS]
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Tendler, Miriam
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Id: lil-295892
Autor: Ramos, Celso Raul Romero; Vilar, Mônica Magno; Nascimento, Ana Lúcia Tabet Oller; Ho, Paulo Lee; Thaumaturgo, Nilton; Edelenyi, Ricardo; Almeida, Marília; Dias, Waldely de Oliveira; Diogo, Catia Maria; Tendler, Miriam.
Título: r-Sm14 - pRSETA efficacy in experimental animals
Fonte: Mem. Inst. Oswaldo Cruz;96(suppl):131-135, Sept. 2001. ilus, tab.
Idioma: en.
Resumo: Previous studies carried out with Sm14 in experimental vaccination against Schistosoma mansoni or Fasciola hepatica infections were performed with recombinant Sm14 (rSm14) produced in Escherichia coli by the pGEMEX system (Promega). The rSm14 was expressed as a 40 kDa fusion protein with the major bacteriophage T7 capsid protein. Vaccination experiments with this rSm14 in animal models resulted in consistent high protective activity against S. mansoni cercariae challenge and enabled rSm14 to be included among the vaccine antigens endorsed by the World Health Organization for phase I/II clinical trials. Since the preparation of pGEMEX based rSm14 is time consuming and results in low yield for large scale production, we have tested other E. coli expression systems which would be more suitable for scale up and downstream processing. We expressed two different 6XHis-tagged Sm14 fusion proteins in a T7 promoter based plasmids. The 6XHis-tag fusions allowed rapid purification of the recombinant proteins through a Ni+2-charged resin. The resulted recombinant 18 and 16 kDa proteins were recognized by anti-Sm14 antibodies and also by antiserum against adult S. mansoni soluble secreted/excreted proteins in Western-Blot. Both proteins were also protective against S. mansoni cercariae infection to the same extent as the rSm14 expressed by the pGEMEX system
Descritores: Schistosoma mansoni/imunologia
Proteínas Recombinantes
Anticorpos Anti-Helmínticos/fisiologia
Proteínas de Helminto/fisiologia
-Plasmídeos
Proteínas Recombinantes/isolamento & purificação
Proteínas de Transporte
Proteínas de Helminto/isolamento & purificação
Western Blotting
Sequência de Aminoácidos
Vacinação
DNA Complementar
Modelos Animais
Eletroforese em Gel de Poliacrilamida
Escherichia coli
Ácidos Graxos
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-886202
Autor: Yeda, Xiao; Shaoqing, Lei; Yayi, Huang; Bo, Zhao; Huaxin, Wang; Hong, Cao; Zhongyuan, Xia.
Título: Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the P38-MAPK/TXNIP signaling activation in streptozotocin induced diabetic rats
Fonte: Acta cir. bras;32(6):429-439, June 2017. graf.
Idioma: en.
Projeto: The Basic Scientific Research of Central University Fund.
Resumo: Abstract Purpose: To determine whether dexmedetomidine (DEX) could attenuate acute kidney injury (AKI) induced by ischemia/reperfusion (I/R) in streptozotocin (STZ)-induced diabetic rats. Methods: Four groups each containing six rats were created (sham control(S), diabetes-sham (DS), diabetes I/R (DI/R), and diabetes-I/R-dexmedetomidine (DI/R-DEX). In diabetes groups, single-dose (65 mg/kg) STZ was administered intraperitoneally (i.p.). In Group DI/R, ischemia reperfusion was produced via 25 min of bilateral renal pedicle clamping followed by 48 h of reperfusion. In Group DI/R-DEX, 50 μg/kg dexmedetomidine was administered intraperitoneally 30 minutes before ischemia. Renal function, histology, apoptosis, the levels of TNF-α, IL-1β, and oxidative stress in diabetic kidney were determined. Moreover, expression of P38 mitogen-activated protein kinase (P38-MAPK), phosphorylated-P38-MAPK(p-P38-MAPK) and thioredoxin-interacting protein (TXNIP) were assessed. Results: The degree of renal I/R injury was significantly increased in DI/R group compared with S group and DS group. The levels of TNF-α, IL-1β, oxidative stress and apoptosis were found significantly higher in DI/R Group when compared with S Group and DS Group. The protein expression of p-P38-MAPK and TXNIP were significantly increased after I/R. All these changes were reversed by DEX treatment. Conclusion: The renoprotective effects of DEX-pretreatment which attenuates I/R-induced AKI were partly through inhibition of P38-MAPK activation and expression of TXINP in diabetic kidney.
Descritores: Traumatismo por Reperfusão/tratamento farmacológico
Substâncias Protetoras/uso terapêutico
Dexmedetomidina/uso terapêutico
Diabetes Mellitus Experimental/complicações
Rim/efeitos dos fármacos
-Traumatismo por Reperfusão/etiologia
Traumatismo por Reperfusão/metabolismo
Transdução de Sinais/efeitos dos fármacos
Proteínas de Transporte/efeitos dos fármacos
Proteínas de Transporte/metabolismo
Ratos Sprague-Dawley
Estreptozocina
Proteínas Quinases p38 Ativadas por Mitógeno/efeitos dos fármacos
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
Rim/lesões
Rim/patologia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-1087458
Autor: Sandoval, C; Vásquez, B.
Título: Evaluación de reactividad inmunohistoquímica con método del Complejo Avidina­Biotina (ABC) / Evaluation of immunohistochemical reactivity with Avidin-Biotin Complex (ABC) method
Fonte: Int. j. med. surg. sci. (Print);3(3):909-918, sept. 2016. ilus.
Idioma: es.
Resumo: Inmunohistoquímica es toda técnica que permite detectar in situ componentes celulares y extracelulares por medio de anticuerpos específicos, empleando sistemas de detección enzimáticos. Dentro de los métodos inmunohistoquímicos, la técnica del complejo avidina­biotina(ABC) es ampliamente utilizada debido a su alta sensibilidad. El objetivo del presente estudio fueevaluar la reactividad inmunohistoquímica del anticuerpo 4C4.9 para la detección de la proteínaS-100, utilizando el método ABC. Para la evaluación de la reactividad inmunohistoquímica se utilizaron 2 biopsias de piel humana con diagnóstico histopatológico de melanoma maligno nodular ulcerado y nevus melanocítico intradérmico, provenientes del Laboratorio de Investigación en Biotecnología Animal de la Universidad de La Frontera, Temuco, Chile. Se utilizó el Kit VECTASTAIN®como método de detección, la dilución del anticuerpo 4C4.9 fue 1/250 y la temperatura de incubación fue a 4 ºC ó 37 ºC por 18 horas. Para validar la técnica, se realizó un control positivo y otro negativo para 4C4.9. Los resultados de la tinción inmunohistoquímica por el método del complejo ABC mostraron tinción positiva para la proteína S-100, tanto en melanoma maligno nodular ulcerado, como en nevus melanocítico intradérmico, incubados durante 18 horas a 4 ºC ó 37 ºC. Sin embargo, la inmunotinción fue más intensa cuando el anticuerpo primario se incubó a 37 ºC. Para una correcta interpretación de los resultados, es necesario tener en consideración que la reacción antígeno-anticuerpo se ve influenciada por diversos factores, como la concentración del anticuerpo, el tiempo y la temperatura de incubación. En conclusión, nuestros resultados sugieren incubarlas muestras con el primer anticuerpo (4C4.9) en una dilución de 1/250 en agua destilada, incu-bando durante 18 h a 37 ºC. Se recomienda la utilización del anticuerpo 4C4.9 como apoyo al diagnóstico y diagnóstico diferencial.

Immunohistochemistry is anytechnique that can detect cellular and extracellular components in situ by means of specific antibodies,using enzymatic detection systems. Among immunohistochemical methods, the technique ofavidin - biotin complex (ABC) is widely used because of its high sensitivity. The aim of this study was to evaluate the immunohistochemical reactivity of the4C4.9 antibody for detection of S-100 protein using the ABC method. For the evaluation ofimmunohistochemical reactivity 2 biopsies of humanskin were used with histopathological diagnosis ofulcerated malignant melanoma and melanocyticintradermal nevi from the Research Laboratory onAnimal Biotechnology of the Universidad de La Fron-tera, Chile. The Kit VECTASTAIN® was used asdetection method, the dilution the 4C4.9 antibodywas 1/250 and incubation temperature was at 4 °Cor 37 °C for 18 hours. To validate the technique, apositive control and a negative for 4C4.9 was performed. The results of immunohistochemicalstaining by the method of ABC complex showed positive staining for protein S-100 both in ulcerated malignant melanoma and melanocytic intradermalnevi, incubated for 18 hours at 4 °C or 37 °C.However, immunostaining was more intense when the primary antibody was incubated at 37° C. For acorrect interpretation of the results, it is necessary to take into consideration that the antigen-antibody reaction is influenced by various factors such as the concentration of antibody, time and temperature ofincubation. In conclusion, our results suggest incubating the samples with the first antibody (4C4.9)at 1/250 dilution in distilled water, incubating for 18h at 37 ºC. However, immunostaining was moreintense when the primary antibody was incubated at37° C. For a correct interpretation of the results, it isnecessary to take into consideration that antigen-antibody reaction is influenced by various factors suchas the concentration of antibody, time and temperature of incubation. In conclusion, our results suggest incubating the samples with the first antibody(4C4.9) at 1/250 dilution in distilled water, incubating for 18 h at 37 ºC. The use of the antibody 4C4.9 is recommended to support the diagnosis and differential diagnosis.
Descritores: Biotina/metabolismo
Imuno-Histoquímica/métodos
Avidina/metabolismo
Técnicas Imunoenzimáticas/métodos
Especificidade de Anticorpos
-Biotina/química
Avidina/química
Proteínas de Transporte
Tipo de Publ: Estudo Clínico
Responsável: CL61.1 - Biblioteca Central Campus Sur


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Id: biblio-974341
Autor: Kandil, Mona; Khalil, Gihane; El-Attar, Eman; Shehata, Gihan; Hassan, Salwa.
Título: Accuracy of heparin binding protein: as a new marker in prediction of acute bacterial meningitis
Fonte: Braz. j. microbiol;49(supl.1):213-219, 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Background: Cerebrospinal fluid bacterial culture is the gold-standard for confirmation of acute bacterial meningitis, but many cases are not culture confirmed. Antibiotics reduce the chance of a microbiological diagnosis. Objective to evaluate efficacy of Heparin-binding protein in diagnosis of bacterial meningitis. Patients: 30 patients diagnosed with acute bacterial meningitis, 30 viral meningitis, and 30 subjects with normal CSF findings. Design: Diagnosis was based on history, clinical criteria, CSF examination, latex agglutination & culture, and sensitivities and response to therapy. HBP was measured using enzyme-linked immunosorbent technique in both serum & CSF. Results: Cerebrospinal fluid HBP levels averaged 0.82 ± 0.3 ng/mL in controls, 3.3 ± 1.7 ng/mL in viral and 174.8 ± 46.7 ng/mL in bacterial meningitis. Mean serum level was 0.84 ± 0.3 ng/mL in the controls, 3.7 ± 1.9 ng/mL in viral, and 192.2 ± 56.6 ng/mL in bacterial meningitis. Both HBP levels were significantly higher in patients with bacterial meningitis. Cut-offs of 56.7 ng/ml and 45.3 ng/ml in cerebrospinal fluid & serum showed 100% overall accuracy. Even in patients who received prior antibiotics, remained elevated. Conclusion: Serum Heparin-binding protein serves as a non-invasive potential marker of acute bacterial meningitis even in partially treated cases.
Descritores: Proteínas Sanguíneas/líquido cefalorraquidiano
Heparina/metabolismo
Proteínas de Transporte/líquido cefalorraquidiano
Proteínas de Transporte/sangue
Meningites Bacterianas/diagnóstico
Peptídeos Catiônicos Antimicrobianos/líquido cefalorraquidiano
Peptídeos Catiônicos Antimicrobianos/sangue
-Biomarcadores/líquido cefalorraquidiano
Biomarcadores/sangue
Estudos Transversais
Meningites Bacterianas/líquido cefalorraquidiano
Meningites Bacterianas/microbiologia
Meningites Bacterianas/sangue
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: lil-623625
Autor: Rimoldi, M. T; Tenner, A. J; Bobak, D. A; Joiner, K. A.
Título: Enhanced invasion of mononuclear phagocytes by serumtreated Trypanosoma cruzi is due to Clq
Fonte: Mem. Inst. Oswaldo Cruz;83(supl.1):456-458, Nov. 1988.
Idioma: en.
Conferência: Apresentado em: Annual Meeting on Basic Research in Chagas's disease, 15, Apresentado em: Meeting of the Brazilian Society of Protozoology4, Caxambu, 7-10 Nov. 1988.
Descritores: Enzimas Ativadoras do Complemento/fisiologia
Glicoproteínas de Membrana
Proteínas de Transporte
-Complemento C1
Receptores de Hialuronatos
Técnicas de Cultura
Macrófagos/parasitologia
Responsável: BR1.1 - BIREME


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Id: biblio-1022150
Autor: Wang, Zisheng; Zhang, Qihuan; Meng, Fancui; Li, Shuai; Xu, Qiaoqin; Qi, Zhitao.
Título: Characterization of the ligand binding of PGRP-L in half-smooth tongue sole (Cynoglossus semilaevis) by molecular dynamics and free energy calculation
Fonte: Electron. j. biotechnol;31:93-99, Jan. 2018. ilus, graf, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Jiangsu Province; . Biotechnology of Marine Wetland.
Resumo: Background: Peptidoglycan (PGN) recognition proteins (PGRPs) are important pattern recognition receptors of the host innate immune system that are involved in the immune defense against bacterial pathogens. PGRPs have been characterized in several fish species. The PGN-binding ability is important for the function of PGRPs. However, the PGRP-PGN interaction mechanism in fish remains unclear. In the present study, the 3-D model of a long PGRP of half-smooth tongue sole (Cynoglossus semilaevis) (csPGRP-L), a marine teleost with great economic value, was constructed through the comparative modeling method, and the key amino acids involved in the interaction with Lys-type PGNs and Dap-type PGNs were analyzed by molecular dynamics and molecular docking methods. Results: csPGRP-L possessed a typical PGRP structure, consisting of five ß-sheets and four α-helices. Molecular docking showed that the van der Waals forces had a slightly larger contribution than Coulombic interaction in the csPGRP-L-PGN complex. Moreover, the binding energies of csPGRP-L-PGNs computed by MM-PBSA method revealed that csPGRP-L might selectively bind both types of MTP-PGNs and MPP-PGNs. In addition, the binding energy of each residue of csPGRP-L was also calculated, revealing that the residues involved in the interaction with Lys-type PGNs were different from that with Dap-type PGNs. Conclusions: The 3-D structure of csPGRP-L possessed typical PGRP structure and might selectively bind both types of MTP- and MPP-PGNs, which provided useful insights to understanding the functions of fish PGRPs.
Descritores: Língua/imunologia
Linguados/imunologia
Linguados/metabolismo
-Sítios de Ligação
Linguados/genética
Peptidoglicano
Proteínas de Transporte
Receptores Toll-Like
Simulação de Dinâmica Molecular
Simulação de Acoplamento Molecular
Ligantes
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: lil-632975
Autor: Enriori, Pablo J; Vico, Clelia M; Enriori, Carlos L.
Título: El dilema de la obesidad en ambos sexos / Dilemma of obesity in both sexes
Fonte: Acta bioquím. clín. latinoam;38(2):165-171, mar.-jun. 2004.
Idioma: es.
Resumo: En la obesidad glúteo-femoral, las consecuencias metabólicas son comparativamente escasas y los efectos endocrinos resultan directamente ligados al exceso de tejido adiposo. En la obesidad abdominal -en cambio- la actividad hormonal es muy importante: resistencia a la insulina e hiperinsulinemia, aumento de la actividad de los factores de crecimiento insulin-análogos (IGFs), aumento de la producción de testosterona (T), dihidrotestosterona (DHT) y estradiol (E2) "biodisponibles", por disminución de la proteína ligadora de andrógenos y estradiol (GLAE). Estas condiciones sugieren una posible asociación con el cáncer mamario y/o endometrial. La secreción de la hormona de crecimiento (HC) se reduce significativamente en la obesidad, junto con los factores hipotalámicos, hipofisarios y periféricos que contribuyen a la secreción anormal de la HC, jugando así un importante papel en la conformación corporal y en el balance de energía. La leptina circulante, producto que se expresa en los adipocitos con el gen ob, ejerce un efecto estimulante sobre la HC. Finalmente, una serie de pacientes seleccionados por su obesidad han sido identificados con importantes aumentos en los factores de crecimiento con valores descendidos de las proteínas portadoras de los IGFs. La obesidad abdominal se caracteriza también por la hiperinsulinemia de ayuno y una exagerada liberación de la insulina después de la carga de glucosa.

In the gluteo-femoral obesity, the metabolic consequences are comparative scarce and the endocrine effects are directly linked to the excess of adipose tissue. In abdominal obesity the endocrine effects are very important: insulin resistance and hyperinsulinemia, increase of IGF-I activity, increase of active androgen production by ovarian estroma, important reduction of sex-hormone-binding-globulin (SHBG) and increasing "bioavailable" estradiol (E2), testosterone (T) and dihidrotestosterone (DHT). In short, obesity and abnormal endocrinology appear to be associated with the development of endometrium and breast cancer in women. Growth hormone (GH) secretion is markedly reduced in obesity, and hypothalamic, pituitary and peripheral factors may contribute to the abnormal GH secretion. GH plays a critical rol in the regulation of body composition and energy balance. The circulating leptin is a product of specific adipocyte ob-gene that exerts stimulating effect on GH release. Furthermore, selected series of obese patients have shown that high free insulin like growth factor (IGF-I) and low IGF-binding proteins generally increased in overweight subjects. Obesity is also characterized by fasting hyperinsulinemia and exaggerated insulin release after a glucose load. Recently it has also demonstrated that leptine plays an important role in the reproductive system at all levels of the hypothalamus-pituitary-gonadal axis.
Descritores: Hormônio do Crescimento Humano
Androgênios
Obesidade
Obesidade/complicações
-Proteínas de Transporte/análise
Endocrinologia
Insulina/análogos & derivados
Limites: Humanos
Responsável: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: biblio-866000
Autor: Demeda, Clarissa Favero.
Título: Estudo da imunoexpressão dos transportadores de glicose 1 e 3 em carcinomas epidemóides de lábio inferior e sua relação com parâmetros clínico-patológicos / Study of the immunoexpression of glucose transporters 1 and 3 in epidemóides carcinomas of the lower lip and its relationship with clinicopathological parameters.
Fonte: Natal; s.n; ago. 2012. 140 p. (BR).
Idioma: pt.
Tese: Apresentada a UFRN para obtenção do grau de Mestre.
Símbolo: BR.
Descritores: Proteínas de Transporte
Carcinoma de Células Escamosas/etiologia
Carcinoma de Células Escamosas/patologia
Imuno-Histoquímica/métodos
Estadiamento de Neoplasias
-Estatísticas não Paramétricas
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos
BR1264.1; D79, D376e


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Id: lil-764505
Autor: Liu, Huanle; Lv, Jingnan; Qi, Xiuqin; Ding, Yu; Li, Dan; Hu, Longhua; Wang, Liangxing; Yu, Fangyou.
Título: The carriage of the serine-aspartate repeat protein-encodingsdr genes among Staphylococcus aureuslineages
Fonte: Braz. j. infect. dis;19(5):498-502tab.
Idioma: en.
Projeto: National Natural Science Fund of China.
Resumo: ABSTRACTThe serine-aspartate repeat proteins (Sdr) are members of a family of surface proteins and contribute to the pathogenicity of Staphylococcus aureus. Among 288 S. aureus isolates including 158 and 130 associated with skin and soft tissue infections and bloodstream infection, respectively; 275 (95.5%) were positive for at least one of threesdr genes tested. The positivity rates for sdrC, sdrD, and sdrE among S. aureusisolates were 87.8% (253/288), 63.9% (184/288), and 68.1% (196/288), respectively. 224 (77.8%) of 288 isolates were concomitantly positive for two or three sdr genes. There was an association between carriage ofsdrE and methicillin-resistant S. aureus(MRSA) isolates, while the carriage rates of sdrC andsdrD in MRSA isolates were similar to those in methicillin-sensitive S. aureus (MSSA) isolates. The prevalence of co-existence of sdrC and sdrE among MRSA isolates was significantly higher than that among MSSA isolates (p < 0.05). All ST1, ST5, ST7, and ST25 isolates were positive for sdrD. While all ST121 and ST398 isolates were negative for sdrD. All ST59 and ST88 isolates were positive forsdrE. All ST1 isolates were concomitantly positive forsdrC and sdrD. Concomitant carriage ofsdrC, sdrD, and sdrE was found among all ST5, 75.0% (9/12) of ST1, 69.2% (9/13) of ST6, 78.6% (11/14) of ST25, and 90.9% (20/22) of ST88 isolates. sdrD was linked to CC5, CC7 and CC88 isolates, especially CC88 isolates. There was a strong association between the presence of sdrE and CC59, CC88, and CC5 isolates. A significant correlation between concomitant carriage of sdrC, sdrD, and sdrE and CC88 isolates was found.sdrC-positive, sdrD-positive andsdrE-negative gene profile was significantly associated with CC7 clone. There was an association between sdrC-positive,sdrD-negative, and sdrE-positive gene profile and CC59 isolates. A correlation between sdrC-positive,sdrD-negative, and sdrE-negative gene profile and CC121 clone was found. More CC59 isolates carriedsdrC-negative, sdrD-negative, andsdrE-positive gene profile relative to other four CCs isolates. All ST1 and ST5, 95.2% (20/21) of ST188 and 95.2% (20/21) of ST630 isolates were positive for sdrC. Taken together, our investigation indicated that different S. aureus lineages were associated with specific patterns of carriage of sdr genes.
Descritores: Proteínas de Bactérias/genética
Proteínas de Ligação ao Cálcio/genética
Proteínas de Transporte/genética
Staphylococcus aureus Resistente à Meticilina/genética
Infecções Estafilocócicas/microbiologia
-Tipagem de Sequências Multilocus
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-761496
Autor: Zhang, Li-min; Song, Wen; Cui, Hao; Xing, Li-qiang; Du, Hui-bo; Cui, Ying; Chen, Wei-hong; Zhao, Zi-gang; Niu, Chun-yu.
Título: Normal mesenteric lymph ameliorates lipopolysaccharide challenge-induced spleen injury
Fonte: Acta cir. bras;30(9):604-610, Sep. 2015. ilus.
Idioma: en.
Projeto: Foundation of Hundred Innovative Talents in Universities of Hebei Province.
Resumo: PURPOSE: This study was conducted to investigate the effect of normal mesenteric lymph (NML) from mice on the spleen injury induced by lipopolysaccharide (LPS) challenge.METHODS: Mice in the LPS and LPS+NML groups received an intraperitoneal injection of LPS (35 mg/kg) and kept for 6 h.. The mice in the LPS+NML group received NML treatment at 1 h after LPS injection. Afterward, the splenic morphology, the levels of lipopolysaccharide-binding protein (LBP), cluster of differentiation 14 (CD14), phosphorylation mitogen-activated protein kinases (MAPKs), and inflammatory mediators in splenic tissue were investigated.RESULTS:LPS injection induced spleen injury, increased the levels of LBP, CD14, tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and interferon γ (IFN-γ), and decreased the IL-4 content in the spleen. By contrast, NML treatment reversed these changes. Meanwhile, the LPS challenge decreased the phosphorylation levels of p38 MAPK, extracellular regulated protein kinases 1/2, and c-Jun N-terminal kinase (JNK). Moreover, the phosphorylation levels of p38 MAPK and JNK were further decreased by the NML administration.CONCLUSION:rRdThe normal mesenteric lymph treatment alleviated lipopolysaccharide induced spleen injury by attenuating LPS sensitization and production of TNF-α, IL-6, and IFN-γ.
Descritores: Lipopolissacarídeos/administração & dosagem
Linfonodos/transplante
Mesentério
Esplenopatias/terapia
-Proteínas da Fase Aguda/análise
/análise
ANTIGENS, CDCONGENITAL ABNORMALITIES/análise
Proteínas de Transporte/análise
Citocinas/análise
Ensaio de Imunoadsorção Enzimática
Injeções Intraperitoneais
Camundongos Endogâmicos BALB C
Glicoproteínas de Membrana/análise
Quinases de Proteína Quinase Ativadas por Mitógeno/análise
Distribuição Aleatória
Reprodutibilidade dos Testes
Resultado do Tratamento
Limites: Animais
Tipo de Publ: Estudo de Avaliação
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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