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Id: biblio-892869
Autor: Karagüzel, Ersagun; Menteşe, Ahmet; O. Kazaz, Ilke; Demir, Selim; Örem, Asim; Okatan, Ali Ertan; Altay, Diler Us; Yaman, Serap Özer.
Título: SCUBE1: a promising biomarker in renal cell cancer
Fonte: Int. braz. j. urol;43(4):638-643, July-Aug. 2017. tab.
Idioma: en.
Projeto: Karadeniz Technical University.
Resumo: ABSTRACT Purpose To investigate the efficacy of signal peptide-CUB-EGF domain-containing protein 1 (SCUBE-1) as a novel biomarker of renal tumors. Materials and Methods 48 individuals were included in the study. The patient group (Group-1) consisted of 23 subjects diagnosed with renal tumor, and the control group (Group-2) of 25 healthy individuals. Patients diagnosed with renal tumor received surgical treatment consisting of radical or partial nephrectomy. Blood specimens were collected following overnight fasting. Signal peptide-CUB-EGF domain-containing protein 1 (SCUBE-1), soluble urokinase plasminogen activator receptor (suPAR) and carbonic anhydrase IX (CA IX) levels were measured from plasma samples. Patients in groups 1 and 2 were compared in terms of these biochemical parameters. Results The 23-member renal tumor group was made up of 17 (73.91%) male and 6 (26.08%) female patients with a mean age of 58.5±15.7 years (range 25 to 80). The 24-member healthy control group was made up of 16 (64%) male and 9 (36%) female subjects with a mean age of 52.4±9.12 years (range 40 to 67). Analysis revealed significant elevation in SCUBE-1 levels in the renal tumor group (p=0.005). No significant differences were detected between the groups with regard to CA IX or suPAR measurements (p=0.062 vs. p=0.176). Conclusions SCUBE-1 appears to represent a promising biomarker in the diagnosis and follow-up of patients with renal tumor.
Descritores: Carcinoma de Células Renais/sangue
Receptores de Ativador de Plasminogênio Tipo Uroquinase/sangue
Anidrase Carbônica IX/sangue
Neoplasias Renais/sangue
Proteínas de Membrana/sangue
-Proteínas de Ligação ao Cálcio
Carcinoma de Células Renais/diagnóstico
Biomarcadores Tumorais/sangue
Estudos de Casos e Controles
Neoplasias Renais/diagnóstico
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adulto
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


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Id: biblio-1136268
Autor: Paula, Marcus Vinicius Barbosa de; Lira Júnior, Marcos Antônio de Farias; Monteiro, Vivian Costa e Silva Crocco; Souto, Ricardo Peres; Fernandes, César Eduardo; Oliveira, Emerson de.
Título: Evaluation of the fibulin 5 gene polymorphism as a factor related to the occurrence of pelvic organ prolapse
Fonte: Rev Assoc Med Bras (1992);66(5):680-686, 2020. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: SUMMARY OBJECTIVE Pelvic organ prolapse (POP) is a very frequent situation in our population that may lead to a significant decrease in patients' quality of life. Currently, we are looking for predictive factors for the development of POPs; thus, this study seeks to evaluate whether the Fibulin 5 polymorphism (FBLN5) is associated with the occurrence of POP. METHODS This is a cohort study with postmenopausal women who were divided into groups by POP stage: POP stages 0 and I (control group) and POP stages III and IV (case group). Subsequently, analyses of genetic polymorphisms of FBLN5 were performed using the Restriction Fragment Length Polymorphism (RFLP) technique. RESULTS A total of 292 women were included in the study. Pregnancy, parity and vaginal delivery in the patients, as well as in data described in the literature, were related to the occurrence of POP in the univariate analysis. However, after binary logistic regression, home birth and age remained independent risk factors for POP. We found no association between the FBLN5 polymorphism and the occurrence of POP (p = 0.371). CONCLUSION There was no association between the FBLN5 polymorphism and the occurrence of POP in Brazilian women.

RESUMO OBJETIVOS O prolapso de órgãos pélvicos (POP) é uma situação muito frequente em nossa população que pode levar a uma diminuição significativa da qualidade de vida dos pacientes. Atualmente, buscam-se fatores preditivos para o desenvolvimento de POPs e, assim, este estudo correlaciona um polimorfismo de Fibulina 5 (FBLN5) com a ocorrência da doença. MÉTODOS Estudo de coorte com mulheres na pós-menopausa, divididas por grupos pelos estádios 0 e I do POP (grupo controle) e POP III e IV (grupo caso). Posteriormente, análises do polimorfismo genético de FBLN5 foram realizadas utilizando a técnica de Polimorfismo de Comprimento de Fragmentos de Restrição (RFLP). RESULTADOS Um total de 292 mulheres foi incluído no estudo. Gestação, paridade e parto vaginal, como bem descritos na literatura, foram relacionados à ocorrência de POPs na análise univariada. No entanto, após a regressão logística binária, o parto domiciliar e a idade permaneceram como fatores de risco independentes para os POPs. Não encontramos associação deste polimorfismo FBLN5 com a ocorrência de POP (p=0,371). CONCLUSÃO Não houve associação deste polimorfismo FBLN5 com a ocorrência de POPs em mulheres brasileiras.
Descritores: Qualidade de Vida
Proteínas da Matriz Extracelular/genética
Prolapso de Órgão Pélvico
-Polimorfismo Genético
Brasil
Proteínas de Ligação ao Cálcio/genética
Estudos de Coortes
Limites: Humanos
Feminino
Gravidez
Responsável: BR1.1 - BIREME


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Id: biblio-887598
Autor: Ademoglu, Esra Nur; Gorar, Suheyla; Keskin, Muge; Carlioglu, Ayse; Ucler, Rifki; Erdamar, Husamettin; Culha, Cavit; Aral, Yalcin.
Título: Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes
Fonte: Arch. endocrinol. metab. (Online);61(5):455-459, Sept.-Oct. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT Objective To investigate serum nesfatin-1 levels at 24-28 weeks of pregnancy in women newly diagnosed with gestational diabetes and determine the association of nesfatin-1 with several metabolic parameters. Subjects and methods Forty women newly diagnosed with gestational diabetes at 24-28 weeks of pregnancy and 30 healthy pregnant women matched in age and gestational week were included in this cross-sectional study. Serum nesfatin-1 levels were analyzed using ELISA, and the relationship between nesfatin-1 and several metabolic parameters were assessed. Results Serum nesfatin-1 levels were found to be lower in women with gestational diabetes compared to the pregnant women in the control sample (p = 0.020). Multiple linear regression analysis revealed that nesfatin-1 was lower in participants with gestational diabetes independently from gestational age, BMI, HOMA-IR, fasting plasma glucose, and age. In correlation analysis, the only variable that was found to have a statistically significant correlation with nesfatin-1 was gestational age (p = 0.015, r = 0.30). Conclusion Lower nesfatin-1 levels in women with gestational diabetes compared to the control group at 24-28 weeks of gestation draws attention to nesfatin-1 levels in gestational diabetes and motivates further research in this area.
Descritores: Proteínas de Ligação ao Cálcio/sangue
Diabetes Gestacional/sangue
Proteínas de Ligação a DNA/sangue
Proteínas do Tecido Nervoso/sangue
-Ensaio de Imunoadsorção Enzimática
Biomarcadores/sangue
Índice de Massa Corporal
Estudos de Casos e Controles
Estudos Transversais
Jejum/sangue
Idade Gestacional
Diabetes Gestacional/diagnóstico
Nucleobindinas
Teste de Tolerância a Glucose
Limites: Humanos
Feminino
Gravidez
Adulto
Responsável: BR1.1 - BIREME


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Latronico, Ana Claudia
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Id: biblio-1019366
Autor: Canton, Ana Pinheiro Machado; Seraphim, Carlos Eduardo; Brito, Vinicius Nahime; Latronico, Ana Claudia.
Título: Pioneering studies on monogenic central precocious puberty
Fonte: Arch. endocrinol. metab. (Online);63(4):438-444, July-Aug. 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT Pubertal timing in humans is determined by complex interactions including hormonal, metabolic, environmental, ethnic, and genetic factors. Central precocious puberty (CPP) is defined as the premature reactivation of the hypothalamic-pituitary-gonadal axis, starting before the ages of 8 and 9 years in girls and boys, respectively; familial CPP is defined by the occurrence of CPP in two or more family members. Pioneering studies have evidenced the participation of genetic factors in pubertal timing, mainly identifying genetic causes of CPP in sporadic and familial cases. In this context, rare activating mutations were identified in genes of the kisspeptin excitatory pathway (KISS1R and KISS1 mutations). More recently, loss-of-function mutations in two imprinted genes (MKRN3 and DLK1) have been identified as important causes of familial CPP, describing novel players in the modulation of the hypothalamic-pituitary-gonadal axis in physiological and pathological conditions. MKRN3 mutations are the most common cause of familial CPP, and patients with MKRN3 mutations present clinical features indistinguishable from idiopathic CPP. Meanwhile, adult patients with DLK1 mutations present high frequency of metabolic alterations (overweight/obesity, early onset type 2 diabetes and hyperlipidemia), indicating that DLK1 may be a novel link between reproduction and metabolism. Arch Endocrinol Metab. 2019;63(4):438-44
Descritores: Puberdade Precoce/genética
-Fenótipo
Puberdade Precoce/etiologia
Ribonucleoproteínas/genética
Proteínas de Ligação ao Cálcio
Inativação Gênica
Peptídeos e Proteínas de Sinalização Intercelular/genética
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Kisspeptinas/genética
Receptores de Kisspeptina-1/genética
Proteínas de Membrana/genética
Proteínas de Membrana/metabolismo
Metilação
Mutação
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-888945
Autor: Mohammadabadi, MR; Jafari, AHD; Bordbar, F.
Título: Molecular analysis of CIB4 gene and protein in Kermani sheep
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(11):e6177, 2017. tab, graf.
Idioma: en.
Resumo: The human calcium- and integrin-binding protein (CIB) family is composed of CIB1, CIB2, CIB3, and CIB4 proteins and the CIB4 gene affects fertility. Kermani sheep is one of the most important breeds of Iranian sheep breeds. The aim of this study was to analyze for the first time molecular characteristics of the CIB4 gene and protein in Kermani sheep. Different tissues were collected from the Kermani sheep and real time PCR was performed. The PCR products were sequenced, comparative analyses of the nucleotide sequences were performed, a phylogenetic tree was constructed, and different characteristics of CIB4 proteins were predicted. Real time PCR results showed that the CIB4 gene is expressed only in testis of Kermani sheep. The cDNA nucleotide sequence was identical with small tail Han sheep, cattle, goat, camel, horse, dog, mouse and human, respectively 100, 99, 99, 98, 98, 96, 96, and 96%. Hence, it can be suggested that the CIB4 gene plays a role in male fertility. Based on the phylogenetic analysis, sheep CIB4 gene has a close relationship with goat and cattle first, and then with camel and whale. Although we demonstrated that CIB4 is a testis-specific gene, expressed only in the testis and it interacts with other proteins, the mechanisms by which CIB4 expression is regulated need to be elucidated.
Descritores: Proteínas de Ligação ao Cálcio/análise
Proteínas de Ligação ao Cálcio/genética
Ovinos/genética
-Sequência de Aminoácidos
Sequência de Bases
Eletroforese/veterinária
Filogenia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Valores de Referência
Limites: Animais
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Id: biblio-839290
Autor: Silveira, CFSMP; Campos, DHS; Freire, PP; Deus, AF; Okoshi, K; Padovani, CR; Cicogna, AC.
Título: Importance of SERCA2a on early isolated diastolic dysfunction induced by supravalvular aortic stenosis in rats
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(5):e5742, 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Cardiac remodeling is defined as changes in shape and function of the heart in response to aggression (pressure overload). The sarcoplasmic reticulum calcium ATPase cardiac isoform 2a (SERCA2a) is a known factor that influences function. A wide spectrum of studies report a decrease in SERCA2a in heart failure, but none evaluate it's the role in early isolated diastolic dysfunction in supravalvular aortic stenosis (AoS). Our hypothesis was that SERCA2a participates in such dysfunction. Thirty-day-old male Wistar rats (60-80 g) were divided into AoS and Sham groups, which were submitted to surgery with or without aorta clipping, respectively. After 6 weeks, the animals were submitted to echocardiogram and functional analysis by isolated papillary muscle (IPM) in basal condition, hypoxia, and SERCA2a blockage with cyclopiazonic acid at calcium concentrations of 0.5, 1.5, and 2.5 mM. Western-blot analyses were used for SERCA2a and phospholamban detection. Data analysis was carried out with Student's t-test and ANOVA. AoS enhanced left atrium and E and A wave ratio, with preserved ejection fraction. Basal condition in IPM showed similar increases in developed tension (DT) and resting tension (RT) in AoS, and hypoxia was similar between groups. After cyclopiazonic acid blockage, final DT was equally decreased and RT was similar between groups, but the speed of relaxation was decreased in the AoS group. Western-blot was uniform in all evaluations. The hypothesis was confirmed, since functional parameters regarding SERCA2a were changed in the AoS group.
Descritores: Estenose Aórtica Supravalvular/complicações
Hipertrofia Ventricular Esquerda/fisiopatologia
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/fisiologia
Disfunção Ventricular Esquerda/fisiopatologia
-Estenose Aórtica Supravalvular/metabolismo
Proteínas de Ligação ao Cálcio/análise
Colágeno/análise
Diástole/fisiologia
Modelos Animais de Doenças
Ecocardiografia
Ventrículos do Coração/patologia
Ventrículos do Coração/fisiopatologia
Hipertrofia Ventricular Esquerda/etiologia
Hipertrofia Ventricular Esquerda/metabolismo
Hipóxia/metabolismo
Hipóxia/fisiopatologia
Indóis
Contração Miocárdica/fisiologia
Ratos Wistar
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/análise
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/metabolismo
Fatores de Tempo
Disfunção Ventricular Esquerda/etiologia
Disfunção Ventricular Esquerda/metabolismo
Remodelação Ventricular/fisiologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Cicogna, Antonio Carlos
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Id: lil-771049
Autor: Junqueira, Adriana; Cicogna, Antônio Carlos; Engel, Letícia Estevam; Aldá, Maiara Almeida; Tomasi, Loreta Casquel de; Giuffrida, Rogério; Giometti, Inês Cristina; Freire, Ana Paula Coelho Figueira; Aguiar, Andreo Fernando; Pacagnelli, Francis Lopes.
Título: Effects of Growth Hormone on Cardiac Remodeling During Resistance Training in Rats / Efeitos do Hormônio do Crescimento na Remodelação Cardíaca Durante Treinamento Resistido em Ratos
Fonte: Arq. bras. cardiol;106(1):18-25, Jan. 2016. tab, graf.
Idioma: pt.
Resumo: Abstract Background: Although the beneficial effects of resistance training (RT) on the cardiovascular system are well established, few studies have investigated the effects of the chronic growth hormone (GH) administration on cardiac remodeling during an RT program. Objective: To evaluate the effects of GH on the morphological features of cardiac remodeling and Ca2+ transport gene expression in rats submitted to RT. Methods: Male Wistar rats were divided into 4 groups (n = 7 per group): control (CT), GH, RT and RT with GH (RTGH). The dose of GH was 0.2 IU/kg every other day for 30 days. The RT model used was the vertical jump in water (4 sets of 10 jumps, 3 bouts/wk) for 30 consecutive days. After the experimental period, the following variables were analyzed: final body weight (FBW), left ventricular weight (LVW), LVW/FBW ratio, cardiomyocyte cross-sectional area (CSA), collagen fraction, creatine kinase muscle-brain fraction (CK-MB) and gene expressions of SERCA2a, phospholamban (PLB) and ryanodine (RyR). Results: There was no significant (p > 0.05) difference among groups for FBW, LVW, LVW/FBW ratio, cardiomyocyte CSA, and SERCA2a, PLB and RyR gene expressions. The RT group showed a significant (p < 0.05) increase in collagen fraction compared to the other groups. Additionally, the trained groups (RT and RTGH) had greater CK-MB levels compared to the untrained groups (CT and GH). Conclusion: GH may attenuate the negative effects of RT on cardiac remodeling by counteracting the increased collagen synthesis, without affecting the gene expression that regulates cardiac Ca2+ transport.

Resumo Fundamento: Apesar de os efeitos benéficos do treinamento resistido (TR) sobre o sistema cardiovascular estarem bem estabelecidos, poucos estudos têm investigado os efeitos crônicos da administração de hormônio do crescimento (GH) sobre a remodelação cardíaca durante um programa de TR. Objetivo: avaliar os efeitos do GH sobre a remodelação cardíaca em suas características morfológicas e na expressão dos genes do trânsito de Ca2+ em ratos submetidos ao TR. Métodos: Ratos Wistar machos foram divididos em 4 grupos (n = 7 por grupo): controle (CT), GH, TR e TR com GH (TRGH). A dose de GH foi de 0,2 UI/kg, a cada dois dias, por 30 dias. O modelo de TR utilizado foi o salto vertical em água (4 séries de 10 saltos, 3 vezes/semana) durante 30 dias consecutivos. Após o período experimental, as seguintes variáveis foram analisadas: peso corporal final (PCF), peso do ventrículo esquerdo (PVE), razão PVE/PCF, área seccional de cardiomiócitos (ASC), fração de colágeno, creatina quinase fração músculo-cérebro (CK-MB) e expressão gênica de SERCA2a, fosfolambam (PLB) e rianodina (RyR). Resultados: Não houve diferença significativa (p > 0,05) entre os grupos para PCF, PVE, razão PVE/PCF, ASC, e expressão gênica de SERCA2a, PLB e RyR. O grupo TR mostrou um significativo aumento (p < 0,05) da fração de colágeno em comparação aos outros. Além disso, os grupos treinados (TR e TRGH) apresentaram maiores níveis de CK-MB em comparação aos não treinados (CT e GH). Conclusão: Esses resultados indicam que o GH pode atenuar os efeitos negativos do TR na remodelação cardíaca por contrabalançar o aumento da síntese de colágeno, sem afetar a expressão de genes que regulam o trânsito de Ca2+ cardíaco.
Descritores: Hormônio do Crescimento/farmacologia
Treinamento de Força/métodos
Remodelação Ventricular/efeitos dos fármacos
-Peso Corporal
Proteínas de Ligação ao Cálcio/análise
Cálcio/metabolismo
Colágeno/análise
Colágeno/efeitos dos fármacos
Creatina Quinase Forma BB/sangue
Creatina Quinase Forma BB/efeitos dos fármacos
Expressão Gênica
Ventrículos do Coração/efeitos dos fármacos
Miócitos Cardíacos/efeitos dos fármacos
Tamanho do Órgão
Reação em Cadeia da Polimerase
Ratos Wistar
Rianodina/análise
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/análise
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/efeitos dos fármacos
Fatores de Tempo
Remodelação Ventricular/genética
Limites: Animais
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: lil-764505
Autor: Liu, Huanle; Lv, Jingnan; Qi, Xiuqin; Ding, Yu; Li, Dan; Hu, Longhua; Wang, Liangxing; Yu, Fangyou.
Título: The carriage of the serine-aspartate repeat protein-encodingsdr genes among Staphylococcus aureuslineages
Fonte: Braz. j. infect. dis;19(5):498-502tab.
Idioma: en.
Projeto: National Natural Science Fund of China.
Resumo: ABSTRACTThe serine-aspartate repeat proteins (Sdr) are members of a family of surface proteins and contribute to the pathogenicity of Staphylococcus aureus. Among 288 S. aureus isolates including 158 and 130 associated with skin and soft tissue infections and bloodstream infection, respectively; 275 (95.5%) were positive for at least one of threesdr genes tested. The positivity rates for sdrC, sdrD, and sdrE among S. aureusisolates were 87.8% (253/288), 63.9% (184/288), and 68.1% (196/288), respectively. 224 (77.8%) of 288 isolates were concomitantly positive for two or three sdr genes. There was an association between carriage ofsdrE and methicillin-resistant S. aureus(MRSA) isolates, while the carriage rates of sdrC andsdrD in MRSA isolates were similar to those in methicillin-sensitive S. aureus (MSSA) isolates. The prevalence of co-existence of sdrC and sdrE among MRSA isolates was significantly higher than that among MSSA isolates (p < 0.05). All ST1, ST5, ST7, and ST25 isolates were positive for sdrD. While all ST121 and ST398 isolates were negative for sdrD. All ST59 and ST88 isolates were positive forsdrE. All ST1 isolates were concomitantly positive forsdrC and sdrD. Concomitant carriage ofsdrC, sdrD, and sdrE was found among all ST5, 75.0% (9/12) of ST1, 69.2% (9/13) of ST6, 78.6% (11/14) of ST25, and 90.9% (20/22) of ST88 isolates. sdrD was linked to CC5, CC7 and CC88 isolates, especially CC88 isolates. There was a strong association between the presence of sdrE and CC59, CC88, and CC5 isolates. A significant correlation between concomitant carriage of sdrC, sdrD, and sdrE and CC88 isolates was found.sdrC-positive, sdrD-positive andsdrE-negative gene profile was significantly associated with CC7 clone. There was an association between sdrC-positive,sdrD-negative, and sdrE-positive gene profile and CC59 isolates. A correlation between sdrC-positive,sdrD-negative, and sdrE-negative gene profile and CC121 clone was found. More CC59 isolates carriedsdrC-negative, sdrD-negative, andsdrE-positive gene profile relative to other four CCs isolates. All ST1 and ST5, 95.2% (20/21) of ST188 and 95.2% (20/21) of ST630 isolates were positive for sdrC. Taken together, our investigation indicated that different S. aureus lineages were associated with specific patterns of carriage of sdr genes.
Descritores: Proteínas de Bactérias/genética
Proteínas de Ligação ao Cálcio/genética
Proteínas de Transporte/genética
Staphylococcus aureus Resistente à Meticilina/genética
Infecções Estafilocócicas/microbiologia
-Tipagem de Sequências Multilocus
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Cicogna, Antonio Carlos
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Id: lil-718100
Autor: Freire, Paula Paccielli; Alves, Carlos Augusto Barnabe; Deus, Adriana Fernandes de; Leopoldo, Ana Paula Lima; Leopoldo, André Soares; Silva, Danielle Cristina Tomaz da; Tomasi, Loreta Casquel de; Campos, Dijon Henrique Salomé; Cicogna, Antonio Carlos.
Título: Obesity does not Lead to Imbalance Between Myocardial Phospholamban Phosphorylation and Dephosphorylation / Obesidade não Acarreta Desequilíbrio entre Fosforilação e Desfosforilação da Fosfolambam Miocárdica
Fonte: Arq. bras. cardiol;103(1):41-50, 07/2014. tab, graf.
Idioma: en.
Resumo: Background: The activation of the beta-adrenergic system promotes G protein stimulation that, via cyclic adenosine monophosphate (cAMP), alters the structure of protein kinase A (PKA) and leads to phospholamban (PLB) phosphorylation. This protein participates in the system that controls intracellular calcium in muscle cells, and it is the primary regulator of sarcoplasmic reticulum calcium pump activity. In obesity, the beta-adrenergic system is activated by the influence of increased leptin, therefore, resulting in higher myocardial phospholamban phosphorylation via cAMP-PKA. Objective: To investigate the involvement of proteins which regulate the degree of PLB phosphorylation due to beta-adrenergic activation in obesity. In the present study, we hypothesized that there is an imbalance between phospholamban phosphorylation and dephosphorylation, with prevalence of protein phosphorylation. Methods: Male Wistar rats were randomly distributed into two groups: control (n = 14), fed with normocaloric diet; and obese (n = 13), fed with a cycle of four unsaturated high-fat diets. Obesity was determined by the adiposity index, and protein expressions of phosphatase 1 (PP-1), PKA, PLB, phosphorylated phospholamban at serine16 (PPLB-Ser16) were assessed by Western blot. Results: Obesity caused glucose intolerance, hyperinsulinemia, hypertriglyceridemia, hyperleptinemia and did not alter the protein expression of PKA, PP-1, PLB, PPLB-Ser16. Conclusion: Obesity does not promote an imbalance between myocardial PLB phosphorylation and dephosphorylation via beta-adrenergic system. .

Fundamento: A ativação do sistema beta-adrenérgico promove a estimulação da proteína G, que, via adenosina monofosfato cíclico (AMPc), altera a estrutura da proteina quinase A (PKA) e acarreta a fosforilação da fosfolambam (PLB). Essa proteína participa do sistema envolvido no controle de cálcio intracelular, em células musculares, sendo a principal reguladora da atividade da bomba de cálcio do retículo sarcoplasmático. Na obesidade ocorre ativação do sistema beta-adrenérgico por influência do aumento da leptina, acarretando, consequentemente, maior fosforilação da fosfolambam miocárdica, via AMPc-PKA. Objetivo: Investigar, na obesidade, o envolvimento das proteínas que regulam o grau de fosforilação do PLB decorrente da ativação beta-adrenérgica. A hipótese do estudo é que há desequilíbrio entre a fosforilação e a desfosforilação da fosfolambam, com predomínio da fosforilação da proteína. Métodos: Ratos Wistar machos foram randomizados e distribuídos em dois grupos: controle (n = 14), alimentado com dieta normocalórica, e obeso (n = 13), com um ciclo de quatro dietas hiperlipídicas insaturadas. A obesidade foi determinada pelo índice de adiposidade, e as expressões proteicas de fosfatase 1 (PP-1), PKA, PLB, fosfolambam fosforilado na serina 16 (pPLB-ser16) foram realizadas por Western Blot. Resultados: A obesidade acarretou intolerância à glicose, hiperinsulinemia, hipertrigliceridemia, hiperleptinemia e não alterou a expressão proteica de PKA, PP-1, PLB, pPLB-ser16. Conclusão: A obesidade não promove desequilíbrio entre a fosforilação e a desfosforilação, via beta-adrenérgica, do PLB miocárdico. .
Descritores: Pressão Sanguínea/fisiologia
Proteínas de Ligação ao Cálcio/metabolismo
Miocárdio/metabolismo
Obesidade/metabolismo
-Glicemia/análise
Colesterol/sangue
Dieta Hiperlipídica
Ácidos Graxos não Esterificados/sangue
Teste de Tolerância a Glucose
Insulina/sangue
Leptina/sangue
Lipoproteínas HDL/sangue
Fosforilação
Distribuição Aleatória
Ratos Wistar
Triglicerídeos/sangue
Remodelação Ventricular/fisiologia
Limites: Animais
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-676272
Autor: Zhang, Heng; Liu, Limei; Yang, Zhong; Pan, Jinhong; Chen, Zhiwen; Fang, Qiang; Li, Weibin; Li, Longkun; Lu, Gengsheng; Zhou, Zhansong.
Título: P2X7 receptor mediates activation of microglial cells in prostate of chemically irritated rats
Fonte: Int. braz. j. urol;39(2):276-285, Mar-Apr/2013. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation.
Resumo: Purpose Evidence shows that adenosine triphosphate (ATP) is involved in the transmission of multiple chronic pain via P2X7 receptor. This study was to investigate the P2X7 and microglial cells in the chronic prostatitis pain. Materials and Methods Rats were divided into control group and chronic prostatitis group (n = 24 per group). A chronic prostatitis animal model was established by injecting complete Freund's adjuvant (CFA) to the prostate of rats, and the thermal withdrawal latency (TWL) was detected on days 0, 4, 12 and 24 (n = 6 at each time point in each group). Animals were sacrificed and the pathological examination of the prostate, detection of mRNA expression of P2X7 and ionized calcium binding adaptor molecule 1 (IBA-1) and measurement of content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the dorsal horn of L5-S2 spinal cord were performed on days 0, 4, 12 and 24. In addition, the content of TNF-α and IL-1β in the dorsal horn of L5-S2 spinal cord was measured after intrathecal injection of inhibitors of microglial cells and/or P2X7 for 5 days. Results The chronic prostatitis was confirmed by pathological examination. The expression of P2X7 and IBA-1 and the content of TNF-α and IL-1β in rats with chronic prostatitis were significantly higher than those in the control group. On day 4, the expressions of pro-inflammatory cytokines became to increase, reaching a maximal level on day 12 and started to reduce on day 24, but remained higher than that in the control group. Following suppression of microglial cells and P2X7 receptor, the secretion of TNF-α and IL-1β was markedly reduced. Conclusion In chronic prostatitis pain, the microglial cells and P2X7 receptor are activated resulting in the increased expression of TNF-α and IL-1β in the L5-S2 spinal cord, which might attribute to the maintenance and intensification of pain in chronic prostatitis. .
Descritores: Microglia/citologia
Microglia/metabolismo
Próstata/metabolismo
Prostatite/metabolismo
/fisiologia
RECEPTORS, PURINERGIC PTEMEFOSXABDOMINAL INJURIES/fisiologia
-Trifosfato de Adenosina/metabolismo
Proteínas de Ligação ao Cálcio/metabolismo
Dor Crônica/metabolismo
Interleucina-1beta/metabolismo
Proteínas dos Microfilamentos/metabolismo
Medição da Dor
Próstata/patologia
Prostatite/patologia
Distribuição Aleatória
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Mensageiro/análise
Medula Espinal/metabolismo
Fator de Necrose Tumoral alfa
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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