Base de dados : LILACS
Pesquisa : D12.776.157.125.806.500 [Categoria DeCS]
Referências encontradas : 1 [refinar]
Mostrando: 1 .. 1   no formato [Detalhado]

página 1 de 1

  1 / 1 LILACS  
             
seleciona
para imprimir
Fotocópia
Texto completo SciELO Chile
Texto completo
Id: biblio-1019509
Autor: Lu, Jinsen; Yang, Jiazhao; Zheng, Yongshun; Fang, Shiyuan; Chen, Xiaoyu.
Título: Resveratrol reduces store-operated Ca2+ entry and enhances the apoptosis of fibroblast-like synoviocytes in adjuvant arthritis rats model via targeting ORAI1-STIM1 complex
Fonte: Biol. Res;52:45, 2019. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: BACKGROUND: Resveratrol was reported to trigger the apoptosis of fibroblast-like synoviocytes In adjuvant arthritis rats but the subcellular mechanism remains unclear. Since ER stress, mitochondrial dysfunction and oxidative stress were involved in the effects of resveratrol with imbalance of calcium bio-transmission, store operated calcium entry (SOCE), a novel intracellular calcium regulatory pathway, may also participate in this process. RESULTS: In the present study, Resveratrol was found to suppress ORAI1 expression of a dose dependent manner while have no evident effects on STIM1 expressive level. Besides, resveratrol had no effects on ATP or TG induced calcium depletion but present partly dose-dependent suppression of SOCE. On the one hand, microinjection of ORAI1 overexpressed vector in sick toe partly counteracted the therapeutic effects of resveratrol on adjuvant arthritis and serum inflammatory cytokine including IL-1, IL-6, IL-8, IL-10 and TNF-α. On the other hand, ORAI1 SiRNA injection provided slight relief to adjuvant arthritis in rats. In addition, ORAI1 overexpression partly diminished the alleviation of hemogram abnormality induced by adjuvant arthritis after resveratrol treatment while ORAI1 knockdown presented mild resveratrol-like effect on hemogram in rats model. CONCLUSION: These results indicated that resveratrol reduced store-operated Ca2+ entry and enhanced the apoptosis of fibroblast-like synoviocytes in adjuvant arthritis rats model via targeting ORAI1-STIM1 complex, providing a theoretical basis for ORAI1 targeted therapy in future treatment with resveratrol on rheumatoid arthritis.
Descritores: Artrite Experimental/fisiopatologia
Canais de Cálcio/efeitos dos fármacos
Apoptose/efeitos dos fármacos
Fibroblastos/efeitos dos fármacos
Sinoviócitos/efeitos dos fármacos
Molécula 1 de Interação Estromal/efeitos dos fármacos
Proteína ORAI1/efeitos dos fármacos
Resveratrol/farmacologia
-Canais de Cálcio/fisiologia
Estresse Oxidativo/efeitos dos fármacos
Resveratrol/administração & dosagem
Mitocôndrias/efeitos dos fármacos
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central



página 1 de 1
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde