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Pesquisa : D12.776.157.530.750.200 [Categoria DeCS]
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Id: lil-491556
Autor: Izaguirre, M. F; Vergara, M. N; Casco, V. H.
Título: CAS role in the brain apoptosis of Bufo arenarum induced by cypermethrin
Fonte: Biocell;30(2):309-320, ago. 2006. ilus, graf.
Idioma: en.
Resumo: CAS might have a key role in the apoptosis induced by toxins, acting as anti-apoptotic factor, stimulating the cellular proliferation and the cell contact stabilization. To start to elucidate their role in the brain apoptosis of Bufo arenarum induced by cypermethrin (CY), the expression patterns of CAS and several cell adhesion molecules (CAMs) were established. Bufo arenarum tadpoles of the control and acute bioassay survival at different doses (39, 156, 625 and 2,500 microg CY/L) and times (24, 48, 72 and 96 h) of CY treatment were fixed in Carnoy, embedded in paraffin and sectioned. CAS and CAMs expression was determined by immunofluorescence and immunohistochemistry, respectively. When the bioassay starts, CAS increases suggesting a proliferative or regenerative effect, but decreases when the doses and/or the bbiocide exposure time increases, suggesting compromise of the cellular cycle control and trigger of an apoptotic wave. However, these neurotoxic mechanisms should not involve degradation of N-cadherin and alpha-catenin, in contrast of beta-catenin and axonal N-CAM180, at least in the initial apoptotic phase. Additionally, an adhesion compensatory mechanism by N-CAM180 is observed in the neuron cell body. These results suggest a dual role of CAS in the cellular cycle control during the CY-induced apoptosis: induction of cell proliferation and stabilization of the cell-cell junctions by modulating CAMs expression.
Descritores: Apoptose
Axônios
Bufo arenarum
Encéfalo/citologia
Encéfalo
Moléculas de Adesão Celular/metabolismo
Proteína de Suscetibilidade a Apoptose Celular/metabolismo
-Bioensaio
Inseticidas/toxicidade
Piretrinas/toxicidade
Análise de Sobrevida
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: AR40.1 - Biblioteca de la Facultad de Ciencias Médicas de la UNCuyo


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Id: lil-394875
Autor: Pagnano, Katia B. B.
Título: Avaliação das proteínas reguladoras do ciclo celular e apoptose nos Linfomas não-Hodgkin agressivos e influência na resposta ao tratamento / Evaluation of cell cycle and apoptosis-regulating proteins in aggressive non-Hodgkin's lymphomas and influence in response to treatment
Fonte: Rev. bras. hematol. hemoter;26(2):149-150, 2004.
Idioma: pt.
Resumo: Os linfomas não-Hodgkin (LNH) agressivos constituemum grupo heterogêneo de neoplasias hematológicas.Os LNH difusos de grandes células B compreendem cercade 20-25% dos LNH. O tratamento quimioterápico podecurar apenas 40%-50% dos pacientes adultos com linfomasagressivos. São considerados indicadores prognósticos:idade, número de sítios extranodais, LDH, performancestatus e estadiamento clínico.Além desses fatores, anormalidades das proteínasreguladoras do ciclo celular e da apoptose parecem ser umimportante mecanismo de desenvolvimento de neoplasiase podem ter um papel no prognóstico dos linfomas agressivos.A expressão das proteínas reguladoras do ciclo celular,p...Avaliamos também a expressão de proteínas reguladorasda apoptose (p53, Bcl-2, Bax, Bak e Mcl-1) de 33pacientes com LNH difusos de grandes células B e analisamosa relação entre a expressão dessas proteínas comdados clínicos e resposta à quimioterapia.Nossos resultados mostraram que a expressão dap53 foi considerada um parâmetro imunohistoquímico independenterelacionado a um pior prognóstico nesseslinfomas. Apesar da alta expressão observada das proteínasBcl-2, Bax, Bak e Mcl-1, não foi encontrado associaçãocom prognóstico ou resposta ao tratamento.

Aggressive non-Hodgkin's lymphomas (NHL) forma heterogeneous group in terms of clinical presentation,histology, immunophenotype, response to treatment andprognosis. Diffuse large B-cell NHL (DLCL) constitute upto 20-25% of NHL in many series. Combinationchemotherapy may cure 40-50% of adult patients. Severalclinical prognostic factors have been described to predictclinical outcome, as age, LDH, performance status andstage and are useful for identifying high-risk patients, whowould benefit from a more intensive approach.Abnormalities of cell cycle and apoptosis regulatingproteins seem to be an important mechanism oftumorigenesis and may play a role in the prognosis ofaggressive NHL.The expression of p53, p21/WAF-1, Mdm2 , c-Mycand proliferating cell nuclear antigen (PCNA) proteins wereexamined by the immunohistochemistry of paraffinembedded samples of 113 high grade non-Hodgkin'slymphomas (NHL) and in 62 patients with aggressive NHLcorrelated to clinical data. Expression of p53, ....NHL patients with a hyper-expression of p53 protein(n=17). DNA extraction was performed in 15 patients andPCR amplification of exons 5-9 was possible in 7 cases.We found a point mutation in exon 6 (Val→Glu;T→A), ina patient with a p53 hyper-expression and p21 negativeexpression.We also evaluated the expression of apoptosisregulatingproteins (p53, Bcl-2, Bax, Bak and Mcl-1) ofparaffin-embedded samples of 33 patients with diffuse largeB-cell NHL, and assessed the relationship of these proteinsto clinical outcome and response to chemotherapy.Our results showed that p53 expression was anindependent immunohistochemical parameter related to apoor prognosis in these lymphomas. Bcl-2, Bax, Bak andMcl-1 proteins, though highly expressed in almost all caseswere not associated with prognosis or response totreatment.
Descritores: Proteína de Suscetibilidade a Apoptose Celular
EVALUATION
Linfoma não Hodgkin
Linfoma não Hodgkin/terapia
Limites: Humanos
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM



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