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Id: lil-732606
Autor: Silva, Bosco Christiano Maciel da; Grassi, Maria Fernanda Rios; Coutinho, Raimundo; Mascarenhas, Rita Elizabeth Moreira; Olavarria, Viviana Nilla; Coutinho-Borgo, Adriana; Kalil, Jorge; Cunha Neto, Edecio; Fonseca, Simone Gonçalves.
Título: Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test
Fonte: Mem. Inst. Oswaldo Cruz;109(8):999-1004, 12/2014. tab, graf.
Idioma: en.
Resumo: The interferon (IFN)-γ response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4+ T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-γ enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-γ production by PBMCs from at least 31% of the TST-positive donors. The magnitude of the response against all peptides was 182 ± 230 x 106 IFN-γ spot forming cells (SFC) among TST-positive donors and 36 ± 62 x 106 SFC among TST-negative donors (p = 0.007). The response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-γ assays to identify individuals with this condition.
Descritores: /imunologia
CDABBREVIATIONS AS TOPIC-POSITIVE T-LYMPHOCYTES/imunologia
Epitopos/imunologia
Interferon gama/metabolismo
Tuberculose Latente/diagnóstico
Mycobacterium tuberculosis/imunologia
Teste Tuberculínico
-Algoritmos
Antígenos de Bactérias/análise
Brasil
Proteínas de Bactérias/sangue
Biomarcadores/análise
/metabolismo
CDABBREVIATIONS AS TOPIC-POSITIVE T-LYMPHOCYTES/metabolismo
Chaperoninas/sangue
ELISPOT
Mapeamento de Epitopos
Voluntários Saudáveis
Antígenos HLA-DR/imunologia
Tuberculose Latente/imunologia
Leucócitos Mononucleares/imunologia
Leucócitos Mononucleares/metabolismo
Proteínas de Ligação a Fosfato/sangue
Limites: Adulto
Humanos
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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