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Pesquisa : D12.776.157.725 [Categoria DeCS]
Referências encontradas : 31 [refinar]
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Id: biblio-1223212
Autor: Wu, Haiying; Li, Sijie; Ji, Minghua; Chen, Qiao; Shi, Jiping; Blamey, Jenny M; Sun, Junsong.
Título: Improvement of polyhydroxybutyrate production by deletion of csrA in Escherichia coli
Fonte: Electron. j. biotechnol;46:8-13, jul. 2020. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: BACKGROUND: Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to be one of critical factors to lower the production cost of PHB in recombinant system. In this study, one of key regulators that affect the cell growth and PHB content was confirmed and analyzed. RESULT: S17-3, a mutant E. coli strain derived from S17-1, was found to be able to achieve high cell density when expressing NphaCAB with the plasmid pBhya-CAB. Whole genome sequencing of S17-3 revealed genetic alternations on the upstream regions of csrA, encoding a global regulator cross-talking between stress response, catabolite repression and other metabolic activities. Deletion of csrA or expression of mutant csrA resulted in improved cell density and PHB content. CONCLUSION: The impact of gene deletion of csrA was determined, dysfunction of the regulators improved the cell density of recombinant E. coli and PHB production, however, the detail mechanism needs to be further clarified.
Descritores: Escherichia coli/metabolismo
Hidroxibutiratos/metabolismo
-Proteínas Repressoras/genética
Biopolímeros/genética
Proteínas Recombinantes
Proteínas de Ligação a RNA/genética
Deleção de Genes
Proteínas de Escherichia coli/genética
Escherichia coli/genética
Engenharia Metabólica
Ligases/metabolismo
Responsável: CL1.1 - Biblioteca Central


  2 / 31 LILACS  
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Id: biblio-892928
Autor: Yang, Zhi-Gang; Ma, Xu-Dong; He, Zhao-Hui; Guo, Ying-xin.
Título: miR-483-5p promotes prostate cancer cell proliferation and invasion by targeting RBM5
Fonte: Int. braz. j. urol;43(6):1060-1067, Nov.-Dec. 2017. graf.
Idioma: en.
Resumo: ABSTRACT Objective: miR-483-5p has been identified as a miRNA oncogene in certain cancers. However, its role in prostate cancer has not been sufficiently investigated. In this study, we investigated the role of miR-483-5p in prostate cancer and examined RBM5 regulation by miR-483-5p. Material and methods: Expression levels of miR-483-5p were determined by quantitative real-time PCR. The effect of miR-483-5p on proliferation was evaluated by MTT assay, cell invasion was evaluated by trans-well invasion assays, and target protein expression was determined by western blotting in LNCaP, DU-145, and PC-3 cells. Luciferase reporter plasmids were constructed to confirm the action of miR-483-5p on downstream target gene RBM5 in HEK-293T cells. Results: we observed that miR-483-5p was upregulated in prostate cancer cell lines and tissues. A miR-483-5p inhibitor inhibited prostate cancer cell growth and invasion in DU-145 and PC-3 cells. miR-483-5p directly bound to the 3' untranslated region (3'UTR) of RBM5 in HEK-293T cells. RBM5 overexpression inhibited prostate cancer cell growth and invasion in LNCaP cells. Enforced RBM5 expression alleviated miR-483-5p promotion of prostate cancer cell growth and invasion in LNCaP cells. Conclusion: The present study describes a potential mechanism underlying a miR-483-5p/RBM5 link that contributes to prostate cancer development.
Descritores: Neoplasias da Próstata/genética
Neoplasias da Próstata/patologia
Regulação Neoplásica da Expressão Gênica/genética
Proteínas de Ciclo Celular/metabolismo
Regiões não Traduzidas/genética
Proteínas Supressoras de Tumor/metabolismo
MicroRNAs/fisiologia
Proliferação de Células/genética
Proteínas de Ligação a DNA/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
-Neoplasias da Próstata/mortalidade
Regulação para Baixo
Regulação para Cima
Proteínas de Ligação a RNA/metabolismo
MicroRNAs/antagonistas & inibidores
Linhagem Celular Tumoral
Invasividade Neoplásica
Limites: Humanos
Masculino
Responsável: BR1.1 - BIREME


  3 / 31 LILACS  
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Texto completo SciELO Chile
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Id: biblio-1131887
Autor: Cao, Danxia; Zhu, Han; Zhao, Qian; Huang, Jianming; Zhou, Cixiang; He, Jianrong; Liang, Yongjun.
Título: MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells
Fonte: Biol. Res;53:43, 2020. tab, graf.
Idioma: en.
Projeto: Pudong Bureau of Health and Family Planning; . National Science Foundation of China.
Resumo: BACKGROUND: Breast cancer, the most common cancer in women worldwide, causes the vast majority of cancer-related deaths. Undoubtedly, tumor metastasis and recurrence are responsible for more than 90 percent of these deaths. MicroRNAs are endogenous noncoding RNAs that have been integrated into almost all the physiological and pathological processes, including metastasis. In the present study, the role of miR-128 in breast cancer was investigated. RESULTS: Compared to the corresponding adjacent normal tissue, the expression of miR-128 was significantly suppressed in human breast cancer specimens. More importantly, its expression level was reversely correlated to histological grade of the cancer. Ectopic expression of miR-128 in the aggressive breast cancer cell line MDA-MB-231 could inhibit cell motility and invasive capacity remarkably. Afterwards, Metadherin (MTDH), also known as AEG-1 (Astrocyte Elevated Gene 1) and Lyric that implicated in various aspects of cancer progression and metastasis, was further identified as a direct target gene of miR-128 and its expression level was up-regulated in clinical samples as expected. Moreover, knockdown of MTDH in MDA-MB-231 cells obviously impaired the migration and invasion capabilities, whereas re-expression of MTDH abrogated the suppressive effect caused by miR-128. CONCLUSIONS: Overall, these findings demonstrate that miR-128 could serve as a novel biomarker for breast cancer metastasis and a potent target for treatment in the future.
Descritores: Neoplasias da Mama/genética
MicroRNAs/fisiologia
MicroRNAs/genética
Invasividade Neoplásica/genética
-Moléculas de Adesão Celular/genética
Moléculas de Adesão Celular/metabolismo
Regulação Neoplásica da Expressão Gênica
Proteínas de Ligação a RNA
Linhagem Celular Tumoral
Proteínas de Membrana
Recidiva Local de Neoplasia
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central


  4 / 31 LILACS  
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Texto completo SciELO Chile
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Id: biblio-1131886
Autor: Yang, Jaehyuk; Lee, Seung Jun; Kwon, Yongseok; Ma, Li; Kim, Jongchan.
Título: Tumor suppressive function of Matrin 3 in the basal-like breast cancer
Fonte: Biol. Res;53:42, 2020. tab, graf.
Idioma: en.
Projeto: Sogang University Research; . Korea government (MSIT).
Resumo: BACKGROUND: Basal-like breast cancer (BLBC) or triple-negative breast cancer (TNBC) is an aggressive and highly metastatic subtype of human breast cancer. The present study aimed to elucidate the potential tumor-suppressive function of MATR3, an abundant nuclear protein, in BLBC/TNBC, whose cancer-relevance has not been characterized. METHODS: We analyzed in vitro tumorigenecity by cell proliferation and soft agar colony formation assays, apoptotic cell death by flow cytometry and Poly (ADP-ribose) polymerase (PARP) cleavage, epithelial-mesenchymal transition (EMT) by checking specific EMT markers with real-time quantitative PCR and in vitro migration and invasion by Boyden Chamber assays. To elucidate the underlying mechanism by which MATR3 functions as a tumor suppressor, we performed Tandem affinity purification followed by mass spectrometry (TAP-MS) and pathway analysis. We also scrutinized MATR3 expression levels in the different subtypes of human breast cancer and the correlation between MATR3 expression and patient survival by bioinformatic analyses of publicly available transcriptome datasets. RESULTS: MATR3 suppressed in vitro tumorigenecity, promoted apoptotic cell death and inhibited EMT, migration, and invasion in BLBC/TNBC cells. Various proteins regulating apoptosis were identified as MATR3-binding proteins, and YAP/TAZ pathway was suppressed by MATR3. MATR3 expression was inversely correlated with the aggressive and metastatic nature of breast cancer. Moreover, high expression levels of MATR3 were associated with a good prognosis of breast cancer patients. CONCLUSIONS: Our data demonstrate that MATR3 functions as a putative tumor suppressor in BLBC/TNBC cells. Also, MATR3 potentially plays a role as a biomarker in predicting chemotherapy-sensitivity and patient survival in breast cancer patients.
Descritores: Genes Supressores de Tumor
Proteínas de Ligação a RNA/genética
Proteínas Associadas à Matriz Nuclear/genética
Neoplasias de Mama Triplo Negativas/genética
-Regulação Neoplásica da Expressão Gênica
Movimento Celular
Apoptose
Linhagem Celular Tumoral
Proliferação de Células
Transição Epitelial-Mesenquimal
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central


  5 / 31 LILACS  
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Id: biblio-950899
Autor: Wu, Tingting; Lin, Yun; Xie, Zhongguo.
Título: MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma
Fonte: Biol. Res;51:13, 2018. graf.
Idioma: en.
Resumo: BACKGROUND: Neuroblastoma (NB) represents the most common extracranial solid tumor in children. Accumulating evidence shows that microRNAs (miRs) play an important role in the carcinogenesis of NB. Here, we investigated the biological function of miR-1247 in NB in vitro. METHODS/RESULTS: We found miR-1247 was downregulated in NB tissues and cells using quantitative PCR analysis. Gain- and loss-of-function studies demonstrated that miR-1247 significantly suppressed cell proliferation and induced cell cycle G0/G1 phase arrest and cell apoptosis of NB cells in vitro by using MTT, colony formation assay and Flow cytometry analysis. Luciferase assay suggested ZNF346 was the target of miR-1247 and its expression could be down-regulated by miR-1247 overexpression using Western blotting. Furthermore, downregulation of ZNF346 by siRNA performed similar effects with overexpression of miR-1247 in NB cells. CONCLUSIONS: Our findings suggested miR-1247 directly targeted to repress ZNF346 expression, thus suppressing the progression of NB, which might be a novel therapeutic target against NB.
Descritores: Proteínas de Ligação a RNA/metabolismo
MicroRNAs/metabolismo
Proteínas de Ligação a DNA/metabolismo
Neuroblastoma/metabolismo
-Fenótipo
Fatores de Tempo
Células Tumorais Cultivadas
Regulação para Baixo
Regulação Neoplásica da Expressão Gênica
Pré-Escolar
Proteínas de Ligação a RNA/genética
Ensaio de Unidades Formadoras de Colônias
MicroRNAs/genética
Proliferação de Células/genética
Proteínas de Ligação a DNA/genética
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Neuroblastoma/genética
Neuroblastoma/patologia
Limites: Humanos
Masculino
Feminino
Responsável: CL1.1 - Biblioteca Central


  6 / 31 LILACS  
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Id: biblio-983940
Autor: Sagredo, Eduardo A; Blanco, Alejandro; Sagredo, Alfredo I; Pérez, Paola; Sepúlveda-Hermosilla, Gonzalo; Morales, Fernanda; Müller, Bettina; Verdugo, Ricardo; Marcelain, Katherine; Harismendy, Olivier; Armisén, Ricardo.
Título: ADAR1-mediated RNA-editing of 3'UTRs in breast cancer
Fonte: Biol. Res;51:36, 2018. graf.
Idioma: en.
Projeto: FONDECYT; . NCI-Leidos; . NIH; . Anillo en Ciencia y Tecnología; . FONDEF; . CORFO.
Resumo: BACKGROUND: Whole transcriptome RNA variant analyses have shown that adenosine deaminases acting on RNA ( ADAR ) enzymes modify a large proportion of cellular RNAs, contributing to transcriptome diversity and cancer evolution. Despite the advances in the understanding of ADAR function in breast cancer, ADAR RNA editing functional consequences are not fully addressed. RESULTS: We characterized A to G(I) mRNA editing in 81 breast cell lines, showing increased editing at 3'UTR and exonic regions in breast cancer cells compared to immortalized non-malignant cell lines. In addition, tumors from the BRCA TCGA cohort show a 24% increase in editing over normal breast samples when looking at 571 well-characterized UTRs targeted by ADAR1. Basal-like subtype breast cancer patients with high level of ADAR1 mRNA expression shows a worse clinical outcome and increased editing in their 3'UTRs. Interestingly, editing was particularly increased in the 3'UTRs of ATM, GINS4 and POLH transcripts in tumors, which correlated with their mRNA expression. We confirmed the role of ADAR1 in this regulation using a shRNA in a breast cancer cell line (ZR-75-1). CONCLUSIONS: Altogether, these results revealed a significant association between the mRNA editing in genes related to cancer-relevant pathways and clinical outcomes, suggesting an important role of ADAR1 expression and function in breast cancer.
Descritores: Neoplasias da Mama/genética
Adenosina Desaminase/genética
Proteínas de Ligação a RNA/genética
Edição de RNA/genética
Regiões não Traduzidas/genética
Estabilidade de RNA/genética
-Neoplasias da Mama/metabolismo
Regulação Neoplásica da Expressão Gênica
Adenosina Desaminase/metabolismo
Proteínas de Ligação a RNA/metabolismo
Perfilação da Expressão Gênica
Estabilidade de RNA/fisiologia
Linhagem Celular Tumoral
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central


  7 / 31 LILACS  
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Id: biblio-1052041
Autor: Qu, Jingwen; Guo, Haiyan; Li, Yongjun; Wang, Qiang; Yin, Xiuyuan; Sun, Xiaomei; Ji, Dejun.
Título: Expression of CPEB1 gene affects the cycle of ovarian granulosa cells from adult and young goats
Fonte: Electron. j. biotechnol;39:74-81, may. 2019. tab, ilus, graf.
Idioma: en.
Projeto: Key Natural Science Program of Jiangsu Higher Education Institutions; . Priority Academic Program Development of Jiangsu Higher Education Institutions.
Resumo: Background: CPEB is considered as an RNA-binding protein first identified in Xenopus oocytes. Although CPEB1 was involved in the growth of oocyte, its role in goat follicular granulosa cell has not been fully elucidated. To clarify the functions of this gene in goat follicular granulosa cells, CPEB1-overexpressing vector and interference vector were structured and transfected into follicular granulosa cells from Jiangsu native white goats of Nantong city, Jiangsu Province, China. The expression levels of differentiation-related genes including CDK1, Cyclin B1, and C-mos were determined 24 h after administration of CPEB1 by quantitative real-time polymerase chain reaction and Western blotting methods. Results: The expression levels of CDK1, Cyclin B1, and C-mos were significantly upregulated after overexpression and significantly downregulated after interference with CPEB1. Conclusions: The CPEB1 gene expression could affect the transcription of genes related to early cleavage divisions, which provided a reference for further research on its role in the growth and maturation of oocytes.
Descritores: Oócitos
Fatores de Transcrição/genética
Cabras/genética
-Transfecção
Fertilização In Vitro
Expressão Gênica
Western Blotting
Reação em Cadeia da Polimerase/métodos
Proteínas de Ligação a RNA
Transferência Embrionária
Gado
Fluorescência
Células da Granulosa
Limites: Animais
Feminino
Responsável: CL1.1 - Biblioteca Central


  8 / 31 LILACS  
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Id: biblio-889115
Autor: Qiu, Zhenpeng; Zhou, Junxuan; Zhang, Cong; Cheng, Ye; Hu, Junjie; Zheng, Guohua.
Título: Antiproliferative effect of urolithin A, the ellagic acid-derived colonic metabolite, on hepatocellular carcinoma HepG2. 2. 15 cells by targeting Lin28a/let-7a axis
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;51(7):e7220, 2018. tab, graf.
Idioma: en.
Projeto: Hubei Provincial Department of Education.
Resumo: An abnormality in the Lin28/let-7a axis is relevant to the progression of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC), which could be a novel therapeutic target for this malignant tumor. The present study aimed to investigate the antiproliferative and anti-invasive effects of urolithin A in a stable full-length HBV gene integrated cell line HepG2.2.15 using CCK-8 and transwell assays. The RNA and protein expressions of targets were assessed by quantitative PCR and western blot, respectively. Results revealed that urolithin A induced cytotoxicity in HepG2.2.15 cells, which was accompanied by the cleavage of caspase-3 protein and down-regulation of Bcl-2/Bax ratio. Moreover, urolithin A suppressed the protein expressions of Sp-1, Lin28a, and Zcchc11, and elevated the expression of microRNA let-7a. Importantly, urolithin A also regulated the Lin28a/let-7a axis in transient HBx-transfected HCC HepG2 cells. Furthermore, urolithin A decelerated the HepG2.2.15 cell invasion, which was involved in suppressing the let-7a downstream factors HMGA2 and K-ras. These findings indicated that urolithin A exerted the antiproliferative effect by regulating the Lin28a/let-7a axis and may be a potential supplement for HBV-infected HCC therapy.
Descritores: Proteínas de Ligação a RNA/efeitos dos fármacos
Carcinoma Hepatocelular/tratamento farmacológico
Cumarínicos/farmacologia
MicroRNAs/efeitos dos fármacos
Neoplasias Hepáticas/tratamento farmacológico
-Valores de Referência
Sincalida/análise
Fatores de Tempo
Replicação Viral/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Western Blotting
Reprodutibilidade dos Testes
Análise de Variância
Proteínas de Ligação a RNA/análise
Carcinoma Hepatocelular/genética
Carcinoma Hepatocelular/virologia
MicroRNAs/análise
Proliferação de Células/efeitos dos fármacos
Células Hep G2
Reação em Cadeia da Polimerase em Tempo Real
Neoplasias Hepáticas/genética
Neoplasias Hepáticas/virologia
Limites: Humanos
Responsável: BR1.1 - BIREME


  9 / 31 LILACS  
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Mendonça, Berenice B
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Id: biblio-840026
Autor: Sousa, Braian Lucas A; Nishi, Mirian Yumie; Santos, Mariza Gerdulo; Brito, Vinicius Nahime; Domenice, Sorahia; Mendonca, Berenice B.
Título: Mutation analysis of NANOS3 in Brazilian women with primary ovarian failure
Fonte: Clinics;71(12):695-698, Dec. 2016. tab, graf.
Idioma: en.
Projeto: CNPq; . CNPq; . FAPESP.
Resumo: OBJECTIVES: Primary ovarian failure is a rare disorder, and approximately 90% of cases are of unknown etiology. The aim of this study was to search for mutations in NANOS3, a gene that was recently related to the etiology of primary ovarian failure, in a group of Brazilian women. METHODS: We screened for NANOS3 DNA variants in 30 consecutive women who were previously diagnosed with primary ovarian failure, of unknown etiology and compared the results with those from 185 women with normal fertility. The NANOS3 gene was amplified by polymerase chain reaction using pairs of specific primers and then sequenced. The resulting sequences were compared with control sequences available in the National Center for Biotechnology and Information database. RESULTS: No mutations in NANOS3 were found in primary ovarian failure patients, but four previously described polymorphisms were identified at a similar frequency in the control and primary ovarian failure groups. CONCLUSIONS: Mutations in NANOS3 were not associated with primary ovarian failure in the present cohort.
Descritores: Proteínas de Ligação a RNA/genética
Insuficiência Ovariana Primária/genética
Mutação
-Polimorfismo Genético
Brasil
Análise Mutacional de DNA
Estudos de Casos e Controles
Reação em Cadeia da Polimerase
Estudos de Coortes
Sequência de Aminoácidos
Eletroforese/métodos
Alelos
Limites: Humanos
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME


  10 / 31 LILACS  
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Id: biblio-979548
Autor: Ruíz, Elizabeth; Augusto-Ramírez, César; Casas, Julián Camilo; Ospina, María Isabel; Requena, José María; Puerta, Concepción J; Salcedo-Reyes, Juan Carlos.
Título: Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing / Caracterización de las regiones no traducidas ARN [UTR] de las isoformas de Trypanosoma cruzi LYT1 derivadas de un trans-empalme alternativo / Caracterização das regiões não-traduzidas do RNA [UTR] das isoformas de Trypanosoma cruzi LYT1 derivadas de uma junção trans-alternativa
Fonte: Univ. sci;23(2):267-290, May-Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract In trypanosomatids, gene expression is mainly regulated at posttranscriptional level, through mechanisms based on the interaction between RNA Binding Proteins [RBPs] and motifs present in the untranslated regions [UTRs] of the mRNAs, which altogether form ribonucleoproteic complexes [RNP] that define the fate of the mRNA. The pre-mRNA derived from the LYT1 gene of Trypanosoma cruzi, is processed by alternative trans-splicing, resulting in different mRNAs which code for the isoforms mLYTl and kLYTl, proteins having differential expression, cellular location and function. The aim of this study was to characterize the 5' and 3' UTRs of the LYT1 mRNAs as the initial step towards the objective of identification of the RBPs responsible for their differential expression. The presence of the two types of 5' UTRs were confirmed in two T. cruzi isolates belonging to the DTU I, thus, corroborating the occurrence of alternative trans-splicing also in the LYT1 gene of this T. cruzi DTU. In addition, for the first time, was unscovered the existence of two types of LYT1 mRNAs transcripts, differing in length by 116 nts, that are generated by alternative polyadenylation. Furthermore, an in-silico analysis of the experimentally obtained UTRs, and ten additional LYT1 sequences retrieved from TritrypDB and GenBank databases, together with a thoroughly search of structural motifs, showed a remarkable conservation of relevant structural motifs previously associated with RNA metabolism in the different UTRs; these elements might be involved in the differential stage-specific expression of each LYT1 isoform.

Resumen En los trypanosomátidos, la expresión génica se regula principalmente en el nivel post-transcripcional mediante mecanismos basados en la interacción entre las proteínas de unión del ARN [RBP] y las figuras presentes en las regiones no traducidas [UTR] de las ARN, que en conjunto forman complejos ribonucleoproteicos [RNP] que definen el destino de la ARN. El pre-ARN derivado del gen LYT1 del Trypanosoma cruzi es procesado por trans-empalme alternativo, dando como resultado diferentes ARN que codifican las isoformas mLYTl y kLYTl, proteínas con expresión diferencial, localización celular y función. El objetivo de este estudio fue caracterizar los 5' y 3' UTR de las ARN LYT1 como el paso inicial hacia la identificación de los RPB responsables de la expresión diferencial. Se confirmó la presencia de los dos tipos de 5' UTR en dos aislantes del T. cruzi pertenecientes al DTU I; de esta forma también se comprobó la ocurrencia del trans-empalme alternativo en el gen LYT1 de este T. cruzi DTU. Además, por primera vez, se pudo demostrar la existencia de dos tipos de transcripciones de ARN LYT1, que difieren en longitud por 116 nts, y son generadas por poliadenilación alternativa. Adicionalmente, se realizó un análisis in-silico de la UTR obtenida experimentalmente, y otras diez secuencias LYT1 recuperadas de las bases de datos TritrypDB y GenBank, junto con una búsqueda exhaustiva de figuras estructuradas, mostrando una notable conservación de los figuras estructurales asociadas con el metabolismo del ARN en los diferentes UTR; estos elementos podrían estar implicados en la expresión diferenciada de la etapa específica de cada isoforma LYT1.

Resumo Nos tripanossomatídeos, a expressão génica é regulada principalmente a nível pós-transcricional mediante mecanismos baseados na interação entre as proteínas de união do RNA [RBPs] e as fugiras presentes nas regiões não-traduzidas [UTRs] do RNA. O pré-RNA derivado do gene LYT1 do Trypanosoma cruzí é processado por uma junção trans-alternativa, resultando em diferentes RNA que codificam as isoformas mLYTl e kLYTl, proteínas com expressão, localização celular e função diferenciadas. O objetivo de este estudo foi caracterizar as 5' e 3' UTRs dos RNAs LYT1 como sendo o passo inicial na identificação das RBPs responsáveis pela expressão diferenciada. A presença dos dois tipos de 5' UTRs foi confirmada em dois isolados de T. cruzí pertencentes ao DTU I; corroborando assim com a ocorrência da junção trans-alternativa no gene LYT1 de este T. crují DTU. Adicionalmente, se demonstrou pela primeira vez a existência de dois tipos de transcrições de RNA LYT1, que se diferenciam em comprimento por 116 nts, e são geradas por poliadenização alternativa. Além disso, realizou-se uma análise in-sílico da UTR obtida experimentalmente e outras dez sequencias LYT1 recuperadas das bases de dados TritrypDB e GenBank, junto com uma busca exaustiva de figuras estruturadas, mostrando uma notável conservação das figuras estruturais associadas com o metabolismo do RNA nas diferentes UTRs. Estes elementos poderiam estar envolvidos na expressão estágio-específica diferenciada de cada isoforma LYT1.
Descritores: Trypanosoma cruzi
-Regulação da Expressão Gênica
Proteínas de Ligação a RNA
Regiões não Traduzidas
Limites: Humanos
Responsável: CO185.1 - Biblioteca Alfonso Borrero Cabal, S. J.



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