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Id: lil-505768
Autor: Ribon, Andréa de O. B; Ribeiro, João Batista; Gonçalves, Daniel B; Queiroz, Marisa V. de; Araújo, Elza F. de.
Título: Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene
Fonte: Genet. mol. biol;32(1):129-132, 2009. ilus.
Idioma: en.
Resumo: Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high mobility complex that was subsequently confirmed by analyzing it with a double-stranded oligonucleotide spanning the CCAAT motif. A substitution in the core sequence for GTAGG partially abolished the formation of specific complexes, showing the involvement of the CCAAT box in the regulation of the polygalacturonase gene studied.
Descritores: Fator de Ligação a CCAAT
Penicillium/genética
Poligalacturonase/genética
-Ensaio de Desvio de Mobilidade Eletroforética
Genes Fúngicos
Regiões Promotoras Genéticas
Fatores Estimuladores Upstream
Responsável: BR26.1 - Biblioteca Central



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