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Id: biblio-1011394
Autor: Xing, Feng; Song, Zhijiao; He, Yuanying.
Título: MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
Fonte: Biol. Res;51:50, 2018. graf.
Idioma: en.
Resumo: BACKGROUND: Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gene expression and their dysregulation has been implicated in tumorigenesis. The aim of this study was to investigate the potential involvement of a specific miRNA, miR-219-5p, in HMGA2-induced ovarian cancer. METHODS: The ovarian cancer cell line, SKOV3, was employed, and miR-219-5p and HMGA2 overexpression vectors constructed. The CCK-8 kit was used to determine cell proliferation and the Transwell® assay used to measure cell invasion and migration. RT-PCR and western blot analyses were applied to analyze the expression of miR-219-5p and HMGA2, and the luciferase reporter assay used to examine the interactions between miR-219-5p and HMGA2. Nude mice were employed to characterize in vivo tumor growth regulation. RESULTS: Expression of miR-219-5p led to suppression of proliferation, invasion and migration of the ovarian cancer cell line, SKOV3, by targeting HMGA2. The inhibitory effects of miR-219-5p were reversed upon overexpression of HMGA2. Data from the luciferase reporter assay showed that miR-219-5p downregulates HMGA2 via direct integration with its 3'-UTR. Consistent with in vitro findings, expression of miR-219-5p led to significant inhibition of tumor growth in vivo. CONCLUSION: Our results collectively suggest that miR-219-5p inhibits tumor growth and metastasis by targeting HMGA2.
Descritores: Neoplasias Ovarianas/metabolismo
Neoplasias Ovarianas/patologia
Regulação Neoplásica da Expressão Gênica/genética
Proteína HMGA2/metabolismo
MicroRNAs/fisiologia
-Neoplasias Ovarianas/genética
Movimento Celular/genética
Proteína HMGA2/genética
Linhagem Celular Tumoral
Proliferação de Células/genética
Invasividade Neoplásica
Metástase Neoplásica
Limites: Humanos
Animais
Feminino
Camundongos
Responsável: CL1.1 - Biblioteca Central



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