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Id: biblio-1147118
Autor: Relloso, María S; Nievas, Jimena; Fares Taie, Santiago; Farquharson, Victoria; Mujica, María T; Romano, Vanesa; Zarate, Mariela S; Smayevsky, Jorgelina.
Título: Evaluación de la espectrometría de masas: MALDI-TOF MS para la identificación rápida y confiable de levaduras / Evaluation of mass spectrometry: MALDI-TOF MS for fast and reliable yeast identification
Fonte: Rev. argent. microbiol;47(2):103-107, June 2015.
Idioma: es.
Resumo: La espectrometría de masas, conocida como matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), es una técnica utilizada en la identificación de microorganismos mediante la creación de un espectro basado en el perfil de proteínas, que es único para una especie dada. El objetivo del presente trabajo fue evaluar la identificación de aislamientos clínicos de levaduras por MALDI-TOF MS en un hospital universitario de Argentina y analizar 2 procedimientos para la extracción de proteínas: el recomendado por el fabricante del equipo y una técnica abreviada rápida. Utilizando el primero de estos procedimientos se analizaron 201 aislamientos identificados previamente por métodos convencionales y se obtuvo coincidencia en la identificación a nivel de especie en el 95,38% de los aislamientos analizados. Con 100 de estos aislamientos se utilizó, además, el procedimiento abreviado para la extracción de proteínas; se obtuvo una identificación correcta a nivel de género y especie en el 98,0% de ellos. La espectrometría de masas MALDI-TOF MS demostró ser una técnica rápida, sencilla y precisa para la identificación de levaduras

The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry technique known as MALDI-TOF MS is a tool used for the identification of clinical pathogens by generating a protein spectrum that is unique for a given species. In this study we assessed the identification of clinical yeast isolates by MALDI-TOF MS in a university hospital from Argentina and compared two procedures for protein extraction: a rapid method and a procedure based on the manufacturer's recommendations. A short protein extraction procedure was applied in 100 isolates and the rate of correct identification at genus and species level was 98.0%. In addition, we analyzed 201 isolates, previously identified by conventional methods, using the methodology recommended by the manufacturer and there was 95.38% coincidence in the identification at species level. MALDI TOF MS showed to be a fast, simple and reliable tool for yeast identification
Descritores: Espectrometria de Massas/métodos
Leveduras/isolamento & purificação
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
-Proteínas Fúngicas/análise
Estudo de Avaliação
Tipo de Publ: Estudo Comparativo
Relatório Técnico
Estudo de Avaliação
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Id: biblio-1042513
Autor: Soares, Alexandra Martins dos Santos; Wanderley, Lêdia Feitosa; Costa Junior, Livio Martins.
Título: The potential of plant and fungal proteins in the control of gastrointestinal nematodes from animals / Potencial de proteínas de plantas e fungos no controle de nematoides gastrintestinais de animais
Fonte: Rev. bras. parasitol. vet;28(3):339-345, July-Sept. 2019.
Idioma: en.
Resumo: Abstract Gastrointestinal nematode infection is an important cause of high economic losses in livestock production. Nematode control based on a synthetic chemical approach is considered unsustainable due to the increasing incidence of anthelmintic resistance. Control alternatives such as the use of natural products are therefore becoming relevant from an environmental and economic point of view. Proteins are macromolecules with various properties that can be obtained from a wide range of organisms, including plants and fungi. Proteins belonging to different classes have shown great potential for the control of nematodes. The action of proteins can occur at specific stages of the nematode life cycle, depending on the composition of the external layers of the nematode body and the active site of the protein. Advances in biotechnology have resulted in the emergence of numerous protein and peptide therapeutics; however, few have been discussed with a focus on the control of animal nematodes. Here, we discuss the use of exogenous proteins and peptides in the control of gastrointestinal.

Resumo A infecção por nematoides gastrintestinais é uma importante causa de grandes perdas econômicas na pecuária. O controle de nematoides com compostos químicos sintéticos é considerado insustentável devido ao aumento da resistência anti-helmíntica. Alternativas de controle, como o uso de produtos naturais, estão se tornando relevantes do ponto de vista ambiental e econômico. As proteínas são macromoléculas com várias propriedades que podem ser obtidas de uma ampla gama de organismos, incluindo plantas e fungos. Proteínas pertencentes a diferentes classes têm mostrado grande potencial para o controle de nematoides. A ação das proteínas pode ocorrer em estágios específicos do ciclo de vida do nematoide, dependendo da composição das camadas externas do parasito e do sítio ativo da proteína. Avanços na biotecnologia resultaram no surgimento de numerosas terapias de proteínas e peptídeos; no entanto, pouco foi discutido com foco no controle de nematoides parasitos de animais. Na presente revisão foi discutido o uso de proteínas exógenas e peptídeos no controle de nematoides gastrintestinais, os mecanismos sugeridos de ação, e os desafios e perspectivas para o uso dessas biomoléculas como uma classe de anti-helmínticos.
Descritores: Peptídeos/isolamento & purificação
Proteínas de Plantas/isolamento & purificação
Proteínas Fúngicas/isolamento & purificação
Gastroenteropatias/veterinária
Infecções por Nematoides/veterinária
Antinematódeos/isolamento & purificação
-Peptídeo Hidrolases/administração & dosagem
Peptídeo Hidrolases/isolamento & purificação
Peptídeos/administração & dosagem
Proteínas de Plantas/administração & dosagem
Biotecnologia
Proteínas Fúngicas/administração & dosagem
Quitinases/administração & dosagem
Quitinases/isolamento & purificação
Gastroenteropatias/parasitologia
Infecções por Nematoides/tratamento farmacológico
Antinematódeos/administração & dosagem
Limites: Animais
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


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Id: lil-435784
Autor: Furlani, Regina Prado Zanes; Godoy, Helena Teixeira.
Título: Valor nutricional de cogumelos comestíveis: uma revisão / Nutritional value of edible mushrooms: a revision
Fonte: Rev. Inst. Adolfo Lutz;64(2):149-154, jul.-dez. 2005.
Idioma: pt.
Resumo: Os cogumelos têm sido tratados como uma iguaria e podem ser apreciados tanto pelas suas características gastronômicas, conferindo sabor e aroma, como também pelo seu valor nutricional. Para a caracterização nutricional de um alimento um trabalho utilizando metodologias adequadas de análises deve ser realizado. Esta revisão apresenta dados de diversos autores, nacionais e internacionais, que realizaram análises quantitativas da composição de cogumelos comestíveis, avaliando o valor nutricional e as diferenças entre meios de cultivos e espécies.
Descritores: Agaricales
Estudos de Avaliação como Assunto
Proteínas Fúngicas/análise
Valor Nutritivo
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-951810
Autor: Rungrattanakasin, Budsayachat; Premjet, Siripong; Thanonkeo, Sudarat; Klanrit, Preekamol; Thanonkeo, Pornthap.
Título: Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis
Fonte: Braz. j. microbiol;49(3):647-655, July-Sept. 2018. graf.
Idioma: en.
Resumo: Abstract An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. The full-length open reading frame of the endoglucanase gene from A. fumigatus DBiNU-1, designated Cel7, was 1383 nucleotides in length and encoded a protein of 460 amino acid residues. The predicted molecular weight and the isoelectric point of the A. fumigatus Cel7 gene product were 48.19 kDa and 5.03, respectively. A catalytic domain in the N-terminal region and a fungal type cellulose-binding domain/module in the C-terminal region were detected in the predicted polypeptide sequences. Furthermore, a signal peptide with 20 amino acid residues at the N-terminus was also detected in the deduced amino acid sequences of the endoglucanase from A. fumigatus DBiNU-1. The endoglucanase from A. fumigatus DBiNU-1 was successfully expressed in K. lactis, and the purified recombinant enzyme exhibited its maximum activity at pH 5.0 and 60 °C. The enzyme was very stable in a pH range from 4.0 to 8.0 and a temperature range from 30 to 60 °C. These features make it suitable for application in the paper, biofuel, and other chemical production industries that use cellulosic materials.
Descritores: Aspergillus fumigatus/enzimologia
Proteínas Fúngicas/genética
Proteínas Fúngicas/química
Expressão Gênica
Celulase/genética
Celulase/química
Clonagem Molecular
-Aspergillus fumigatus/genética
Especificidade por Substrato
Estabilidade Enzimática
Kluyveromyces/genética
Kluyveromyces/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Proteínas Recombinantes/química
Proteínas Fúngicas/metabolismo
Celulase/metabolismo
Temperatura Alta
Concentração de Íons de Hidrogênio
Responsável: BR1.1 - BIREME


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Id: biblio-1039268
Autor: Cardoso, Wilton Soares; Queiroz, Paula Viana; Tavares, Gabriella Peterlini; Santos, Fernando Almeida; Soares, Filippe Elias de Freitas; Kasuya, Maria Catarina Megumi; Queiroz, José Humberto de.
Título: Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material
Fonte: Braz. j. microbiol;49(4):879-884, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.
Descritores: Proteínas Fúngicas/química
Sorghum/química
Celulases/química
Fungos/enzimologia
Lignina/química
-Proteínas Fúngicas/metabolismo
Caules de Planta/microbiologia
Caules de Planta/química
Sorghum/microbiologia
Celulases/metabolismo
Biocatálise
Fungos/química
Hidrólise
Lignina/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-974323
Autor: Wang, Xiuwen; Zhang, Xiaohua; Yao, Qingshou; Hua, Dongliang; Qin, Jiayang.
Título: Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol
Fonte: Braz. j. microbiol;49(supl.1):160-165, 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Shandong Province Science and Technology Project; . Jinan Youth Science and Technology Star Project.
Resumo: Abstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.
Descritores: Ascomicetos/metabolismo
Proteínas Fúngicas/metabolismo
Diterpenos/metabolismo
-Ascomicetos/genética
Ascomicetos/química
Proteínas Fúngicas/química
Carbono/metabolismo
Eletroforese em Gel Bidimensional
Regulação Fúngica da Expressão Gênica
Proteômica
Glucose/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-974310
Autor: Souza, Renata Carolini; Cantão, Maurício Egídio; Nogueira, Marco Antonio; Vasconcelos, Ana Tereza Ribeiro; Hungria, Mariangela.
Título: Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach
Fonte: Braz. j. microbiol;49(4):723-730, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: CNPq-Universal; . CNPq; . Embrapa.
Resumo: ABSTRACT The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the tillage system, with the NT showing about five times more hydrolases than the CT system. The outstanding differences can be attributed to the management of crop residues, left on the soil surface in the NT, and mechanically broken and incorporated into the soil in the CT. Differences between the CS and the CR were slighter, 10% higher for the CS, but not statistically different. Most of the sequences belonged to fungi (Verticillium, and Colletotrichum for lipases and laccases, and Aspergillus for proteases), and to the archaea Sulfolobus acidocaldarius for amylases. Our results indicate that agricultural soils under conservative managements may represent a hotspot for bioprospection of hydrolases.
Descritores: Solo/química
Proteínas Fúngicas/genética
Archaea/enzimologia
Proteínas Arqueais/genética
Fungos/enzimologia
Hidrolases/genética
-Microbiologia do Solo
Soja/crescimento & desenvolvimento
Triticum/crescimento & desenvolvimento
Brasil
Archaea/isolamento & purificação
Archaea/classificação
Archaea/genética
Zea mays/crescimento & desenvolvimento
Agricultura
Metagenoma
Metagenômica
Fungos/isolamento & purificação
Fungos/classificação
Fungos/genética
Responsável: BR1.1 - BIREME


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Id: biblio-974295
Autor: Vieira, Gabriela A. L; Magrini, Mariana Juventina; Bonugli-Santos, Rafaella C; Rodrigues, Marili V. N; Sette, Lara D.
Título: Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process
Fonte: Braz. j. microbiol;49(4):749-756, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: ABSTRACT Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Therefore, this study aimed to select one PAHs degrader among three marine-derived basidiomycete fungi and to study its pyrene detoxification/degradation. Marasmiellus sp. CBMAI 1062 showed higher levels of pyrene and BaP degradation and was subjected to studies related to pyrene degradation optimization using experimental design, acute toxicity, organic carbon removal (TOC), and metabolite evaluation. The experimental design resulted in an efficient pyrene degradation, reducing the experiment time while the PAH concentration applied in the assays was increased. The selected fungus was able to degrade almost 100% of pyrene (0.08 mg mL-1) after 48 h of incubation under saline condition, without generating toxic compounds and with a TOC reduction of 17%. Intermediate metabolites of pyrene degradation were identified, suggesting that the fungus degraded the compound via the cytochrome P450 system and epoxide hydrolases. These results highlight the relevance of marine-derived fungi in the field of PAH bioremediation, adding value to the blue biotechnology.
Descritores: Hidrocarbonetos Policíclicos Aromáticos/metabolismo
Água do Mar/microbiologia
Basidiomycota/metabolismo
-Filogenia
Hidrocarbonetos Policíclicos Aromáticos/química
Pirenos/metabolismo
Pirenos/química
Basidiomycota/isolamento & purificação
Basidiomycota/classificação
Basidiomycota/genética
Benzo(a)pireno/metabolismo
Benzo(a)pireno/química
Biodegradação Ambiental
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Responsável: BR1.1 - BIREME


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Id: biblio-974344
Autor: Hernández-Monjaraz, Wendy Susana; Caudillo-Pérez, César; Salazar-Sánchez, Pedro Ulises; Macías-Sánchez, Karla Lizbeth.
Título: Influence of iron and copper on the activity of laccases in Fusarium oxysporum f. sp. lycopersici
Fonte: Braz. j. microbiol;49(supl.1):269-275, 2018. tab, graf.
Idioma: en.
Projeto: Instituto Politécnico Nacional, Secretaría de Investigación y Posgrado.
Resumo: ABSTRACT Fusarium oxysporum f. sp. lycopersici is a phytopathogenic fungus that causes vascular wilt in tomato plants. In this work we analyze the influence of metal salts such as iron and copper sulphate, as well as that of bathophenanthrolinedisulfonic acid (iron chelator) and bathocuproinedisulfonic acid (copper chelator) on the activity of laccases in the intra (IF) and extracellular fractions (EF) of the wild-type and the non-pathogenic mutant strain (rho1::hyg) of F. oxysporum. The results show that laccase activity in the IF fraction of the wild and mutant strain increased with the addition of iron chelator (53.4 and 114.32%; respectively). With copper, it is observed that there is an inhibition of the activity with the addition of CuSO4 for the EF of the wild and mutant strain (reduction of 82 and 62.6%; respectively) and for the IF of the mutant strain (54.8%). With the copper chelator a less laccase activity in the IF of the mutant strain was observed (reduction of 53.9%). The results obtained suggest a different regulation of intracellular laccases in the mutant strain compared with the wild type in presence of CuSO4 and copper chelator which may be due to the mutation in the rho gene.
Descritores: Proteínas Fúngicas/metabolismo
Cobre/metabolismo
Lacase/metabolismo
Fusarium/enzimologia
Ferro/metabolismo
-Doenças das Plantas/microbiologia
Proteínas Fúngicas/genética
Proteínas Fúngicas/química
Lycopersicon esculentum/microbiologia
Lacase/genética
Lacase/química
Fusarium/genética
Fusarium/química
Responsável: BR1.1 - BIREME


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Id: biblio-828206
Autor: Lopes Leal, Patrícia; Varón-López, Maryeimy; Gonçalves de Oliveira Prado, Isabelle; Valentim dos Santos, Jessé; Fonsêca Sousa Soares, Cláudio Roberto; Siqueira, José Oswaldo; de Souza Moreira, Fatima Maria.
Título: Enrichment of arbuscular mycorrhizal fungi in a contaminated soil after rehabilitation
Fonte: Braz. j. microbiol;47(4):853-862, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Spore counts, species composition and richness of arbuscular mycorrhizal fungi, and soil glomalin contents were evaluated in a soil contaminated with Zn, Cu, Cd and Pb after rehabilitation by partial replacement of the contaminated soil with non-contaminated soil, and by Eucalyptus camaldulensis planting with and without Brachiaria decumbens sowing. These rehabilitation procedures were compared with soils from contaminated non-rehabilitated area and non-contaminated adjacent soils. Arbuscular mycorrhizal fungi communities attributes were assessed by direct field sampling, trap culture technique, and by glomalin contents estimate. Arbuscular mycorrhizal fungi was markedly favored by rehabilitation, and a total of 15 arbuscular mycorrhizal fungi morphotypes were detected in the studied area. Species from the Glomus and Acaulospora genera were the most common mycorrhizal fungi. Number of spores was increased by as much as 300-fold, and species richness almost doubled in areas rehabilitated by planting Eucalyptus in rows and sowing B. decumbens in inter-rows. Contents of heavy metals in the soil were negatively correlated with both species richness and glomalin contents. Introduction of B. decumbens together with Eucalyptus causes enrichment of arbuscular mycorrhizal fungi species and a more balanced community of arbuscular mycorrhizal fungi spores in contaminated soil.
Descritores: Solo/química
Microbiologia do Solo
Brasil
Micorrizas/classificação
Poluição Ambiental
-Poluentes do Solo/química
Esporos Fúngicos
Proteínas Fúngicas
Contagem de Colônia Microbiana
Metais Pesados/química
Responsável: BR1.1 - BIREME



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