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Id: lil-793952
Autor: Morales-Camacho, Jocksan I; Paredes-López, Octavio; Espinosa-Hernández, Edgar; Fernández Velasco, Daniel Alejandro; Luna-Suárez, Silvia.
Título: Expression, purification and thermal stability evaluation of an engineered amaranth protein expressed in Escherichia coli
Fonte: Electron. j. biotechnol;19(4):44-51, July 2016. ilus.
Idioma: en.
Projeto: Secretaría de Investigación y Posgrado-IPN; . Consejo Nacional de Ciencia y Tecnología.
Resumo: Background: The acidic subunit of amarantin (AAC)-the predominant amaranth seed storage protein-has functional potential and its third variable region (VR) has been modified with antihypertensive peptides to improve this potential. Here, we modified the C-terminal in the fourth VR of AAC by inserting four VY antihypertensive peptides. This modified protein (AACM.4) was expressed in Escherichia coli. In addition, we also recombinantly expressed other derivatives of the amarantin protein. These include: unmodified amarantin acidic subunit (AAC); amarantin acidic subunit modified at the third VR with four VY peptides (AACM.3); and amarantin acidic subunit doubly modified, in the third VR with four VY peptides and in the fourth VR with the RIPP peptide (AACM.3.4). Results: E. coli BL21-CodonPlus (DE3)-RIL was the most favorable strain for the expression of proteins. After 6 h of induction, it showed the best recombinant protein titer. The AAC and AACM.4 were obtained at higher titers (0.56 g/L) while proteins modified in the third VR showed lower titers: 0.44 g/L and 0.33 g/L for AACM.3 and AACM.3.4, respectively. As these AAC variants were mostly expressed in an insoluble form, we applied a refolding protocol. This made it possible to obtain all proteins in soluble form. Modification of the VR 4 improves the thermal stability of amarantin acidic subunit; AAC manifested melting temperature (Tm) at 34°C and AACM.4 at 37.2°C. The AACM.3 and AACM.3.4 did not show transition curves. Conclusions: Modifications to the third VR affect the thermal stability of amarantin acidic subunit.
Descritores: Proteínas de Plantas/metabolismo
Amaranthus
-Proteínas de Plantas/isolamento & purificação
Proteínas de Plantas/química
Temperatura
Engenharia de Proteínas
Western Blotting
Reatores Biológicos
Subunidades Proteicas
Eletroforese em Gel de Poliacrilamida
Escherichia coli
Estabilidade Proteica
Fermentação
Globulinas
Responsável: CL1.1 - Biblioteca Central


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Id: lil-527521
Autor: Majolino, Ignazio; Severino, Alessandro.
Título: Allogeneic transplantation in multiple myeloma / Transplante alogênico no mieloma múltiplo
Fonte: Rev. bras. hematol. hemoter;31(supl.2):35-40, ago. 2009.
Idioma: en.
Resumo: In this review the authors present a state of art tretment of multiple myeloma.High dose chemo-radiotherapy followed by autologous hematopoietic stem cell transplantation has been show to be superior a conventional chemotherapy and a double transplantation. The authors discuss too, the allogeneic transplantation with reduced intensity conditioning, allogeneic versus tandem autologous, results the patients long term outcome and a approach about the use of donor lymphocytes, anti thimocyte globulin and a overview of post transplant therapies.

Neste relato os autores apresentam uma revisão sobre o estado atual do tratamento mieloma múltiplo. São enfatizados aspectos sobre a vantagem do transplante autólogo em seguimento à quimioterapia convencional e o duplo transplante. São discutidos o transplante alogênico e o condicionamento com intensidade reduzida, além do uso de linfócitos do doador, da globulina antitimocítica e uma visão geral do futuro da terapia da moléstia.
Descritores: Mieloma Múltiplo
-Pacientes
Terapêutica
Doadores de Tecidos
Transplante Autólogo
Transplante Homólogo
Linfócitos
Transplante de Células-Tronco Hematopoéticas
Tratamento Farmacológico
Globulinas
Tipo de Publ: Revisão
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: biblio-1049076
Autor: Espinosa-Hernández, Edgar; Morales-Camacho, Jocksan Ismael; Fernández-Velasco, D. Alejandro; Benítez-Cardoza, Claudia G; Rosas-Cárdenas, Flor de Fátima; Luna-Suárez, Silvia.
Título: The insertion of bioactive peptides at the C-terminal end of an 11S globulin changes the structural stability and improves the antihypertensive activity
Fonte: Electron. j. biotechnol;37:18-24, Jan. 2019. tab, ilus, graf.
Idioma: en.
Projeto: Secretaría de Investigación y Posgrado IPN, México; . Consejo Nacional de Ciencia y Tecnología, México.
Resumo: BACKGROUND: The 11S globulin from amaranth is the most abundant storage protein in mature seeds and is well recognized for its nutritional value. We used this globulin to engineer a new protein by adding a four valinetyrosine antihypertensive peptide at its C-terminal end to improve its functionality. The new protein was named AMR5 and expressed in the Escherichia coli BL21-CodonPlus(DE3)-RIL strain using a custom medium (F8PW) designed for this work. RESULTS: The alternative medium allowed for the production of 652 mg/L expressed protein at the flask level, mostly in an insoluble form, and this protein was subjected to in vitro refolding. The spectrometric analysis suggests that the protein adopts a ß/α structure with a small increment of α-helix conformation relative to the native amaranth 11S globulin. Thermal and urea denaturation experiments determined apparent Tm and C1/2 values of 50.4°C and 3.04 M, respectively, thus indicating that the antihypertensive peptide insertion destabilized the modified protein relative to the native one. AMR5 hydrolyzed by trypsin and chymotrypsin showed 14- and 1.3-fold stronger inhibitory activity against angiotensin I-converting enzyme (IC50 of 0.034 mg/mL) than the unmodified protein and the previously reported amaranth acidic subunit modified with antihypertensive peptides, respectively. CONCLUSION: The inserted peptide decreases the structural stability of amaranth 11S globulin and improves its antihypertensive activity.
Descritores: Peptídeos/metabolismo
Proteínas/metabolismo
Globulinas/metabolismo
Anti-Hipertensivos/metabolismo
-Sementes
Temperatura
Meios de Cultura
Amaranthus
Estabilidade Proteica
Compostos Fitoquímicos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-975627
Autor: Koparal, Murat Yavuz; Polat, Fazlı; Çetin, Serhat; Bulut, Ender Cem; Sözen, Tevfik Sinan.
Título: Prognostic role of preoperative albumin to globulin ratio in predicting survival of clear cell renal cell carcinoma
Fonte: Int. braz. j. urol;44(5):933-946, Sept.-Oct. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT Purpose: To investigate the prognostic role of preoperative albumin/globulin ratio (AGR) in predicting disease-free survival (DFS) and overall survival (OS) in localized and locally advanced clear cell renal cell carcinoma (RCC) patients. Patients and Methods: 162 patients who met the criteria specified were included in the study. The DFS and OS ratios were determined using the Kaplan-Meier method. Univariate and multivariate Cox regression analyses were performed to determine the prognostic factors affecting DFS and OS. Results: Median follow-up period was 27.5 (6-89) months. There was a statistically significant relationship between low AGR and high pathological tumor (pT) stage, presence of collecting system invasion, presence of tumor necrosis, and a high platelet count (p = 0.012, p = 0.01, p = 0.001, and p = 0.004, respectively). According to the Kaplan-Meier survival analysis, both OS and DFS were found to be significantly lower in the low AGR group (p = 0.006 and p = 0.012). In the multivariate Cox regression analysis, collecting system invasion and tumor necrosis were found to be independent prognostic factors in predicting OS and pT stage was found to be an independent prognostic factor in predicting DFS (HR: 4.08, p = 0.043; HR: 8.64, p = 0.003 and HR: 7.78, p = 0.041, respectively). Conclusion: In our study, low AGR was found to be associated with increased mortality and disease recurrence in localized and locally advanced RCC.
Descritores: Albumina Sérica/análise
Carcinoma de Células Renais
Globulinas/análise
Neoplasias Renais/sangue
-Prognóstico
Carcinoma de Células Renais/cirurgia
Carcinoma de Células Renais/mortalidade
Biomarcadores Tumorais/sangue
Valor Preditivo dos Testes
Estudos Retrospectivos
Sensibilidade e Especificidade
Intervalo Livre de Doença
Neoplasias Renais/cirurgia
Neoplasias Renais/mortalidade
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1152188
Autor: Motta, Oswaldo Jesus Rodrigues da; Gomes, Andreia Patricia; Oliveira, Leandro Almeida de; Oliveira, Leonardo de Almeida; Almeida, Caio Henrique Santos; Braga, Luciene Muniz.
Título: Hyperimmune globulin and monoclonal antibody for the treatment of SARS CoV-2 infection
Fonte: Rev. patol. trop;49(4), 2020.
Idioma: en.
Resumo: The current pandemic of COVID-19 (Coronavirus Disease-19), caused by the pathogen SARSCoV-2, leading to thousands of deaths in 2020, has mobilized the scientific community, with the purpose of understanding the different aspects of the disease and proposing new treatment and prevention measures. Research carried out in several areas includes evaluating the possibility of using hyperimmune globulin and monoclonal antibodies against SARS CoV-2 as well as analyzing the viability and efficacy of monoclonal antibodies and hyperimmune globulin in combating Sars-CoV-2. A literature review was carried out with a defined search strategy using the keywords (i) "hyperimmune globulin", (ii) "monoclonal antibody", (iii)" covid-19" and (iv)" sars-cov-2", in the PubMed and ResearchGate databases, using the bibliographic references of the articles selected in these databases. Of the 187 citations obtained, 10 articles published between 2019 and 2020 were selected after reading the summary of each one and determining its compatibility with the objective of this study. Final considerations highlight the main challenges and the feasibility of using hyperimmune globulin and monoclonal antibodies for the treatment/prevention of SARS CoV-2 infection.
Descritores: Infecções por Coronavirus
Betacoronavirus
Globulinas
Anticorpos Monoclonais
Limites: Humanos
Tipo de Publ: Relatos de Casos
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas


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Id: biblio-1025554
Autor: Polini, Nélida; Zavatti, Jorge Rubén.
Título: Variabilidad de la eritrosedimentación en sujetos sanos, antes y después de la desfibrinación in vitro / Erythrosedimentation variability in healthy subjects, before and after defibrination in vitro
Fonte: Rev. Asoc. Med. Bahía Blanca;11(1):2-6, abr. 2001.
Idioma: es.
Resumo: El objetivo de este trabajo fue estudiar la variabilidad que pue- de esperarse en la reducción de la eritrosedimentación (ERS) después de la desfibrinación de la muestra de sangre y su correlación con el fibrinógeno plasmático funcional (FF) y las globulinas plasmáticas (GP), en sujetos sanos. De acuerdo al criterio bioantropológico corriente la muestra poblacional se dividió en tres grupos etarios: adolescentes (n=50), juveniles (n=30) y adultos (n=40). Se determinó la ERS antes y después de desfibrinar la muestra de sangre por el método de Westergren, la concentración del FF por el método manual de Clauss en una sola etapa y el contenido de GP por semimicroelectroforesis. El análisis estadístico de los datos permitió determinar que en los juveniles, en ambos sexos, se presentaron los menores valores de la ERS tanto antes como después de desfibrinar la sangre (mujeres: de 2-22 a 0-10 mm/hora; varones: de 0,5-12 a 0-2 mm/hora), incrementando el riesgo de considerar a un sujeto de este grupo sano cuando no lo está. En general, ambas ERS fueron mayores para las mujeres, siendo la diferencia estadísticamente significativa sólo en la edad adulta (p< 0.05). También para este sexo resultó significativamente mayor el descenso en la ERS que provoca el desfibrinado de la muestra en todas las edades estudiadas (p< 0.0001), sin embargo, dicha disminución no esta asociada a los niveles de FF y/o GP observados. Por último, contrariamente a lo que sucede con los enfermos, en los sujetos sanos, no se encontró correlación entre la reducción de la ERS con las concentraciones del FF ni con el contenido de las GP.

The aim of this work was to study the erythrocyte-sedimentation rate (ERS) that may be expected after the defibrination of the blood sample, and its correlation with the functional plasmafibrinogen (FF) and plasmaglobulins (GP) in healthy subjects. The sample population was divided, in accordance with the usual bioanthropological criteria, into three age groups: adolescents (n=50), young adults (n=30) and adults (n=40). The ERS was measured before and after the defibrination of the blood-sample by the Westergren method, the plasma-fibrinogen concentration was estimated by the Clauss one-stage manual method, and the globulin level by means of semimicroelectrophoresis. According to the statistical analysis of the data, young adults irrespective of their sex presen- ted the lowest ERS values both before and after defibrination (women: from 2-22 to 0-10 mm/ h; men: from 0,5-12 to 0-2 mm/ h), a fact which increases the risk of considering a sick patient healthy. In general, both ERS were higher for women, although this difference was statistically relevant only for the adult age group (p<0,05). Mo- reover, as a result of defibrination the females from all the age groups studied featured a significantly greater reduction in their ERS (p<0,0001). However, this fall in rate is associated with neither the FF nor the GP levels observed. Finally, unlike whats occurs with sick patientes, healthy individuals did not present a correlation between the reduction in ERS and either the FF concentration or the globulin level.
Descritores: Fibrinogênio
-Desfibriladores
Globulinas
Limites: Humanos
Tipo de Publ: Estudo Clínico
Responsável: AR393.1 - Centro de Información y Documentación Dr H. Urquiola


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Id: lil-631772
Autor: Padilla, Fanny C; Guédez, Thanee; Alfaro, María J; Regnault, Miriam; Rincón C, Alicia M.
Título: Fraccionamiento y caracterización de las proteínas solubles de la harina de nuez de Barinas (caryodendron orinocense K. ) / Fractionation and characterization of soluble proteins from nuez de Barinas (caryodendron orinocense K .) flour
Fonte: Rev. Inst. Nac. Hig;41(1):38-42, jun. 2010. graf, tab.
Idioma: es.
Resumo: La harina de nuez de Barinas (Caryodendron orinocense K.) de acuerdo a estudios realizados presenta un contenido de pro teínas en el rango de 15-18%, que permite se pueda considerar como fuente de proteína. Sin embargo no se conoce la composición de estas proteínas. En este estudio se realizó un proceso de fraccionamiento de acuerdo a la solubilidad en diferentes solventes (agua, cloruro de sodio al 5%, hidróxido de sodio 0,02N, etanol al 70%), y el establecimiento de la composición proteica según el peso molecular de la harina de nuez de Barinas obtenida a partir de las nueces secas, molidas y des grasadas, determinando el rango de pesos moleculares por SDS-PAGE y su comparación con el rango de pesos moleculares de las proteínas de la harina de soya (Glycine max). Asi mismo, se determinó la digestibilidad in vitro por hidrólisis enzimática. Los resultados indican un rango de PM de 20.000- 97.000 daltons para la soya y de 6.500-45.000 daltons para la nuez de Barinas. El peso molecular más bajo sugiere una más fácil digestión de las proteínas, que corrobora el valor de la di - ges tibilidad obtenido de 75%. Las proteínas presentes están cons tituidas por albúminas 50,72%, globulinas 15,56%, prolaminas 23,10% y glutelinas 2,52% que representan el 92,15% del total de proteínas presentes. Estas fracciones presentaron una pureza de 81,57%, 94,01%, 70,86% y 92,53%, respectivamente. Estos resultados sugieren la posibilidad de uso de la harina de Caryodendron orinocense K en el desarrollo de productos alimenticios para niños y ancianos.

Nuez de Barinas (Caryodendron orinocense K.) flour has been reported to have a content of proteins in the range of 15-18% that allows considering it as a source of proteins. However, there is no evidence on the composition of these proteins. The objective of this study was the extraction and separation of the different fractions of proteins based on their solubility in different solvents (water, 5% sodium chloride, sodium hydroxide 0.02N, and 70% ethanol), and the protein composition assessment of the nuez de Barinas flour from dried, milled, and defatted nuts; by analysis of the range of mo lecular weight by SDS-PAGE. Molecular weights of proteins from soybean (Glycine max) flour, were used for comparison. In vitro protein digestibility was determined by enzymatic hydrolysis. Results presented soy proteins in the range of 20.000-97.000 daltons while the nuez de Barinas flour proteins were in the range of 6.500-45.000 daltons. Low molecular weight proteins should suggest a much easier digestibility of these proteins, which is related to the digestibility index of 75% found. Protein composition was found to be 50,72% albumins, 15,56% globulins, 23,10% prolamins, and 2,52% glutelins which represent 92,15% of total proteins present. These fractions showed a percent purity of 81.57, 94.01, 70.86 and 92.53, respectively. These results suggest the possibility to use this flour Caryodendron orinocense K. in developing food products for children and elderly persons.
Descritores: Plantas/classificação
Proteínas/uso terapêutico
Albuminas
Prolaminas
-Saúde Pública
Globulinas
Nozes/classificação
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Idoso
Idoso de 80 Anos ou mais
Tipo de Publ: Estudo de Avaliação
Responsável: VE9.1 - Biblioteca


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Id: biblio-889101
Autor: Barros, MAV; Andrade, EB; Barros, RGN; Costa, IKM; Costa, ICL; Vitorino, GFA; Andrade, JJC; Paulino-Silva, KM; Nogueira, VO; de Brito Alves, JL; Costa-Silva, JH.
Título: Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;51(6):e6602, 2018. tab, graf.
Idioma: en.
Projeto: FACEPE; . CNPq.
Resumo: The aim of this study was to investigate the reproductive, biochemical, and hematological outcomes of pregnant rats exposed to protein restriction. Wistar rat dams were fed a control normal-protein (NP, 17% protein, n=8) or a low-protein (LP, 8% protein, n=14) diet from the 1st to the 20th day of pregnancy. On the 20th day, the clinical signs of toxicity were evaluated. The pregnant rats were then anesthetized and blood samples were collected for biochemical-hematological analyses, and laparotomy was performed to evaluate reproductive parameters. No sign of toxicity, or differences (P>0.05) in body weight gain and biochemical parameters (urea, creatinine, albumin, globulin, and total protein) between NP and LP pregnant dams were observed. Similarly, hematological data, including red blood cell count, white blood cell count, hemoglobin, hematocrit, red blood cell distribution width (coefficient of variation), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, % lymphocytes, absolute lymphocyte count, platelet count, and mean platelet volume were similar (P>0.05) at the end of pregnancy. Reproductive parameters (the dam-offspring relationship, ovary mass, placenta mass, number of corpora lutea, implantation index, resorption index, and the pre- and post-implantation loss rates) were also not different (P>0.05) between NP and LP pregnant dams. The present data showed that a protein-restricted diet during pregnancy did not alter reproductive, biochemical, and hematological parameters and seems not to have any toxic effect on pregnant Wistar rats.
Descritores: Adaptação Fisiológica/fisiologia
Dieta com Restrição de Proteínas/métodos
Desenvolvimento Fetal/fisiologia
Genitália Feminina/fisiologia
-Ureia/sangue
Hemoglobinas/análise
Ganho de Peso/fisiologia
Proteínas/análise
Ratos Wistar
Creatinina/sangue
Albuminas/análise
Contagem de Eritrócitos
Globulinas/análise
Hematócrito
Contagem de Leucócitos
Limites: Animais
Masculino
Feminino
Gravidez
Ratos
Responsável: BR1.1 - BIREME


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Costa, D
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Id: biblio-1011266
Autor: Souza, A. F; Schade, J; Ramos, A. F; Albuquerque, M. S. M; Fonteque, G. V; Costa, D; Muller, T. R; Fonteque, J. H.
Título: Serum proteinogram of the Campeiro horse / Proteinograma sérico de equinos da raça Campeiro
Fonte: Arq. bras. med. vet. zootec. (Online);71(2):363-368, mar.-abr. 2019. tab.
Idioma: en.
Resumo: The aim of this study to measure the fractions of the total serum proteins of the Campeiro horse and identify the influences of biological variants. Blood samples were taken in 138 horses of the breed Campeiro for measuring the concentration of total serum protein by the biuret method. Serum concentrations of protein fractions were measured by electrophoresis using agarose gel. Groups were formed according to age, sex and reproductive condition. The average values of serum fractions: albumin (2.85±0.36g/dl), alpha 1 (0.28±0.11g/dl), alpha 2 (0.26±0.08g/dL) beta 1 (0.57±0.15g/dl), beta 2 (0.89±0.28g/dL), gamaglobulinas (1.86±0.34g/dL), albumin/globulin ratio (0.75±0.18) and 2.5% percentile and 97.5% had slight differences in relation to the reference interval proposed for the species. They observed higher values of alpha 1 and 2 globulins in the group from that had six to eight years old and gammaglobulins in group above 13 years old. Serum protein concentrations were similar in horses and mares and between non-pregnant and pregnant. Sex and pregnancy status did not affect serum proteinogram. Alpha and gammaglobulins have higher values as the age increases. Serum proteinogram of Campeiro horses shows variations that have to be considered in the interpretation of laboratory tests.(AU)

Este trabalho tem por objetivo mensurar as frações das proteínas totais séricas de equinos Campeiros e identificar as influências de variantes biológicas. Foram colhidas amostras de sangue de 138 equinos, machos e fêmeas da raça Campeiro. A determinação da concentração de proteínas totais séricas foi realizada pelo método de biureto. As concentrações séricas das frações proteicas foram determinadas por eletroforese, utilizando-se gel de agarose. Formaram-se grupos em relação à idade, ao sexo e à condição reprodutiva. Os valores médios das frações séricas albumina (2,85±0,36g/dL), alfa 1 (0,28±0,11g/dL), alfa 2 (0,26±0,08g/dL), beta 1 (0,57±0,15g/dL), beta 2 (0,89±0,28g/dL), gamaglobulinas (1,86±0,34g/dL), relação albumina/globulina (0,75±0,18) e os percentis 2,5% e 97,5% apresentaram diferenças pontuais em relação aos intervalos propostos para a espécie. Observaram-se maiores valores de alfa 1, alfa 2 globulinas, no grupo de seis a oito anos, e de gamaglobulinas, no grupo acima de 13 anos de idade. O proteinograma sérico foi similar entre machos e fêmeas e entre fêmeas vazias e gestantes. Sexo e estado gestacional não afetaram o proteinograma sérico. Alfa e gamaglobulinas têm incrementos em função de idades crescentes. O proteinograma sérico de equinos Campeiros tem variações que devem ser consideradas em exames laboratoriais.(AU)
Descritores: Cavalos/sangue
-Eletroforese das Proteínas Sanguíneas/veterinária
Albumina Sérica
Globulinas
Limites: Animais
Masculino
Feminino
Gravidez
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Carvalho, Maria Acelina Martins de
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Id: biblio-966300
Autor: Carvalho, Maria Acelina Martins de; Bezerra, Dayseanny de Oliveira; Neves, Camila Arrivabene; Ferraz, Maíra Soares; Carvalho, Yulla Klinger Pereira de; Machado Júnior, Antônio Augusto N; Conde Júnior, Aírton Mendes; Pessoa, Gerson Tavares; Almeida, Hatawa Melo de; Fortes, Eunice Anita Moura; Feitosa, Matheus Levi Tajra; Fortes, Eunice Anita M.
Título: Serum biochemistry in hystricomorpha: agouti (Dasyprocta prymnolopha) during pregnancy / Bioquímica sérica em hystricomorpha: cutia (Dasyprocta prymnolopha, WAGLER, 1831) durante o período gestacional
Fonte: Biosci. j. (Online);33(5):1274-1283, sept./oct. 2017. graf, tab.
Idioma: en.
Resumo: The aim of this estudy was to establish the levels of serum total protein, albumin, globulin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), calcium, phosphorus, urea, creatinine, bilirubin and glucose during pregnancy in agoutis. Animals: Twelve pregnant agouti from the Center for the Study and Preservation of Wild Animals (CSPWA) of the Federal University of Piauí (UFPI) were used in this research. After identification of the estrus, the day of the coverage was confirmed by means of vaginal cytology with the visualization of spermatozoa (day zero) and confirmation of pregnancy by ultrasonographic examination after 15 days. Blood samples were collected by lateral saphenous vein puncture after physical restraint, every 10 days until the end of pregnancy, for biochemical analyzes. A completely randomized experimental design was used and the means compared by the Duncan test at 5% probability using the SAS (Statistical Analysis System). The results of the biochemical analysis of total protein, albumin, globulin, urea, creatinine, calcium, phosphorus, serum ALT, glucose, AST, total bilirubin, direct bilirubin and indirect bilirubin in pregnant agouti (Dasyprocta prymnolopha) did not differ when compared to nonpregnant females. The serum biochemical levels during pregnancy in agoutis, except for calcium and phosphorus, were unchanged compared to those found in the non-pregnant adult animal, as occurs in other species. The changes during pregnancy reflect the physiology and biology of wild species, elucidating information about the biochemical parameters during pregnancy, thus characterizing the animal as a benchmark for comparisons with other species, extolling its importance both for nature conservation and production in capivity.

O estudo objetivou estabelecer os níveis séricos de proteínas totais, albumina, globulina, Alanina Aminotransferase (ALT), Aspartato Aminotransferase (AST), cálcio, fósforo, ureia, creatinina, bilirrubina e glicose durante a gestação em cutias. A pesquisa foi desenvolvida utilizando-se 12 cutias fêmeas criadas no Núcleo de Estudos, Produção e Preservação de Animais Silvestres da Universidade Federal do Piauí. Após a identificação do estro, o dia da cobertura foi confirmado por meio de citologia vaginal com a visualização de espermatozoides (dia zero) e confirmação da gestação por exame ultrassonográfico após 15 dias. Confirmada a gestação, foram coletados 03 mL de sangue mediante punção da veia pudenda interna, após contenção física, a cada 10 dias, até o final da gestação. Foi feita a análise de variância para um delineamento inteiramente casualizado com teste de Duncan para comparação das médias a 5% de probabilidade utilizando-se do programa estatístico SAS (Statistical Analysis System). Os resultados obtidos por meio da análise bioquímica de proteína total, albumina, globulina, ureia, creatinina, cálcio, fósforo, ALT séricas, glicose, aspartato aminotransferase (AST), bilirrubina total, bilirrubina direta e bilirrubina indireta de cutias gestantes (Dasyprocta prymnolopha) diferem de forma absoluta quando comparados a fêmeas não gestantes. Os níveis bioquímicos séricos durante a gestação em cutias, com exceção do cálcio, fósforo, sofrem alterações comparadas ao animal adulto não prenhe, como ocorre em outras espécies. Os níveis nas cutias gestantes sofrem alterações de acordo com o tempo de gestação, com maiores mudanças no período inicial e final da prenhez. As mudanças durante a gravidez refletem a fisiologia e a biologia da espécie silvestre, elucidando informações sobre os parâmetros bioquímicos durante a gestação, caracterizando o animal como referência para comparações com outras espécies, exaltando a importância tanto para sua conservação quanto para a sua produção em cativeiro.
Descritores: Aspartato Aminotransferases
Alanina Transaminase
Albuminas
Dasyproctidae
Globulinas
-Gravidez
Responsável: BR396.1 - Biblioteca Central



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