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Carvalho, Emília Campos de
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Id: lil-761705
Autor: Silva, Natália Chantal Magalhães da; Chaves, Érika de Cássia Lopes; Carvalho, Emilia Campos de; Carvalho, Leonardo César; Iunes, Denise Hollanda.
Título: Foot reflexology in feet impairment of people with type 2 diabetes mellitus: randomized trial / Reflexologia podal no comprometimento dos pés de pessoas com diabetes mellitus tipo 2: ensaio randomizado / Reflexología podal en el comprometimiento de los pies de personas con diabetes mellitus tipo 2: ensayo aleatorio
Fonte: Rev. latinoam. enferm. (Online);23(4):603-610, July-Aug. 2015. tab, ilus.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Resumo: AbstractObjective: to evaluate the effect of foot reflexology on feet impairment of people with type 2 diabetes mellitus.Method: this is a randomized, controlled and blind clinical trial. The sample was comprised by people with type 2 diabetes mellitus who, after being randomized into Treated group (n = 21) and Control group (n = 24), received guidelines on foot self-care. To the Treated Group it was also provided 12 sessions of foot reflexology. The scores of impairment indicators related to skin and hair, blood circulation, tissue sensitivity and temperature were measured by means of the instrument for assessing tissue integrity of the feet of people with diabetes mellitus. Chi-square test, Fisher exact test, Mann-Whitney test and regression analyzes were applied to the data, considering a significance level of 5% (P value <0.05).Results: participants who received the therapy showed better scores in some impairment indicators related to skin and hair (hair growth, elasticity/turgor, hydration, perspiration, texture and integrity of the skin/ skin peeling).Conclusion: the foot reflexology had a beneficial effect on feet impairment of people with type 2 diabetes mellitus, which makes it a viable therapy, deserving investment. This study was registered in the Brazilian Registry of Clinical Trials - RBR-8zk8sz.

ResumoObjetivo:avaliar o efeito da reflexologia podal no comprometimento dos pés de pessoas com diabetes mellitus tipo 2.Método:trata-se de um ensaio clínico, randomizado, controlado e mascarado. A amostra foi composta por pessoas com diabetes mellitus tipo 2 que, após serem randomizadas em grupo Tratado (n=21) e Controle (n=24), receberam orientações de autocuidado com os pés. Ao Grupo Tratado também foram fornecidas 12 sessões de reflexologia podal. Foram mensurados os escores de comprometimento de indicadores relacionados à pele e pelos, circulação sanguínea, sensibilidade e temperatura tissular por meio do Instrumento para avaliação da integridade tissular dos pés de pessoas com diabetes mellitus. Aos dados foram aplicados os testes Qui-Quadrado, Exato de Fisher, Mann-Whitney e Análises de regressão, considerando-se nível de significância de 5% (Valor P<0,05).Resultados:os participantes que receberam a terapia apresentaram melhores escores de comprometimento em alguns indicadores relacionados à pele e pelos (crescimento de pelos, elasticidade/tugor, hidratação, transpiração, textura e integridade da pele/descamação cutânea).Conclusão:a reflexologia podal apresentou efeito benéfico sobre o comprometimento dos pés de pessoas com diabetes mellitus tipo 2, o que a torna uma terapia viável e que merece investimento. Este estudo foi registrado no Registro Brasileiro de Ensaios Clínicos - RBR-8zk8sz.

ResumenObjetivo:evaluar el efecto de la reflexología podal en el comprometimiento de los pies de personas con diabetes mellitus tipo 2.Método:se trata de un ensayo clínico, aleatorio, controlado y enmascarado. La muestra estuvo compuesta por personas con diabetes mellitus tipo 2 que, después de ser tratadas aleatoriamente en los grupos Tratado (n=21) y Control (n=24), recibieron orientaciones de autocuidado de los pies. También, al Grupo Tratado se le suministraron 12 sesiones de reflexología podal. Fueron medidos los puntajes de comprometimiento de indicadores relacionados a la piel y pelos, circulación sanguínea, sensibilidad y temperatura tisular por medio de instrumento para evaluación de la integridad del tejido de los pies de personas con diabetes mellitus. Los datos fueron sometidos a las pruebas Chi-cuadrado, Exacta de Fisher, Mann-Whitney y Análisis de regresión, considerando un nivel de significación de 5% (Valor p<0,05).Resultados:los participantes que recibieron la terapia presentaron mejores puntajes de comprometimiento en algunos indicadores relacionados a la piel y pelos (crecimiento de pelos, elasticidad/turgencia, hidratación, transpiración, textura e integridad de la piel/descamación cutánea).Conclusión:la reflexología podal presentó efecto benéfico sobre el comprometimiento de los pies de personas con diabetes mellitus tipo 2, lo que la torna una terapia viable y que merece inversiones. Este estudio fue registrado en el Registro Brasileño de Ensayos Clínicos - RBR-8zk8sz.
Descritores: Anticorpos Monoclonais Murinos/farmacologia
/imunologia
ANTIGENS, CDABRUPTIO PLACENTAE/imunologia
/imunologia
CDABRUPTIO PLACENTAE LIGAND/imunologia
Sobrevivência de Enxerto/efeitos dos fármacos
Transplante de Coração
Antígeno-1 Associado à Função Linfocitária/imunologia
Glicoproteínas de Membrana/imunologia
Fatores de Necrose Tumoral/imunologia
-Aloenxertos
Rejeição de Enxerto/imunologia
Rejeição de Enxerto/patologia
Rejeição de Enxerto/prevenção & controle
Sobrevivência de Enxerto/imunologia
Molécula 1 de Adesão Intercelular/imunologia
Camundongos Endogâmicos BALB C
Transplante de Pele
Fatores de Tempo
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-623769
Autor: Fischer, A; Blanche, S; Le Deist, F; Veber, F; Griscelli, C; Olive, D; Delaage, M; Mawas, C.
Título: Prevention of bone marrow graft failure by an anti LFA-1 monoclonal antibody
Fonte: Mem. Inst. Oswaldo Cruz;82(supl.2):101-104, 1987. tab.
Idioma: en.
Conferência: Apresentado em: International Symposium on Immunomodulators: Biology and Therapeutic Applications, Rio de Janeiro, Apr. 26-30, 1987.
Resumo: Graft rejection is the major cause of failure of HLA mismatched bone marrow transplantation because of residual host immunity. we have proposed to use a monoclonal murine antibody specific for the LFA-1 molecule (25-3) to prevent graft failure in HLA mismatched bone marrow transplantation (BMT). The rationale for this approach is three fold: LFA-1 deficient patients (3/3) do not reject HLA mismatched BMT; anti LFA-1 blocka in vitro the induction of T cell responses and T/ non T cytotoxic functions; LFA-1 is not expressed by other cells than leucocytes. We have accordingly treated twenty two patients with inherited diseases and 8 with leikemia. The bone marrow was T cells depled by E rosetting of Campath antibody. The antibody was given at days -3, -1, +1, +3, +5 at dose of .1 mg/kg/d for the first 9 and then .2mg/kg/d from day -3 to +6. Engraftment occured in 23/30 patients as shown by at least HLA typing. Hematological recovery was rapid, GVH was limited. Side effects of antibody infusion included fever and possibly an increased incidence of early bacteral infection (sepsis, 1 death). Immunological reconstitution occured slowly leading in six cases to EBV-induced B cell poliferation (1 death and in two others to transient auto immune hemolytic anemia. There has been only one secondary graft rejection. Sisteen patients are alive 3 to 26 months post transplant with functional grafts. Although the number of patients treated is still low the absence of late rejection so far, gives hope for long term maintenance of the graft using anti LFA-1. Since the antibody is an IgG 1 unable to bind human complement, and since it is known to inhibit phagocytosis, there is a good suggestion that 25-3 act through functional blocking of host T and non T luymphocytes at both induction and effector levels.
Descritores: Células da Medula Óssea/citologia
Anticorpos Monoclonais
-Antígeno-1 Associado à Função Linfocitária
Afinidade de Anticorpos
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1006098
Autor: Prieto, Fernanda; Cabañas, Claudia; Villagra, Verónica.
Título: Monitoreo de anticuerpos anti-HLA en pacientes con insuficiencia renal crónica en lista de espera para trasplante / Anti-hla antibodies monitoring in patients with chronic re- nal failure on waiting list for renal transplant in Paraguay
Fonte: Rev. nefrol. diál. traspl;36(2):75-81, mar. 2016. ilus, tab.
Idioma: es.
Conferência: Apresentado em: 53° Congreso ERA-EDTA (Viena, Austria: 21-24 mayo 2016), Viena, 21-24 mayo 2016.
Resumo: INTRODUCCIÓN: El estudio de anticuerpos anti-HLA en el suero del paciente en lista de espera para trasplante renal es fundamental para optimizar la elección de un donante así como el esquema de inmunosupresión de inducción y mantenimiento acorde al riesgo inmunológico. Estos anticuerpos pueden Encontrarse de manera preexistente al trasplante como resultado de exposición del individuo a transfusiones sanguíneas, embarazos y trasplantes previos. El objetivo del estudio fue determinar la incidencia de inmunización frente a antígenos de HLA, los factores asociados y su impacto en pacientes en espera de un trasplante renal. MATERIAL Y MÉTODOS: En este estudio, observacional retrospectivo de corte trasversal, fueron incluidos 254 pacientes en lista de espera para trasplante renal que acudieron al Laboratorio Central de Salud Pública en el período comprendido entre julio de 2013 y julio de 2015. RESULTADOS: De los 254 pacientes estudiados, 30% presentaron anticuerpos anti-HLA. El evento sensibilizante más significativo fue la exposición a un trasplante previo, presentando anticuerpos anti-HLA el 84% de los candidatos a retrasplante (p<0,05). En segundo lugar se encontraron las mujeres multíparas, presentando un PRA (Panel Reactivo de Anticuerpos) positivo el 69% de ellas (p<0,05). Por último, el 24% de los pacientes poli-transfundidos presentaron anticuerpos anti HLA (p<0,05). CONCLUSIONES: En el trascurso de los dos años de estudio, 51 pacientes fueron trasplantados, de los cuales un solo paciente presentaba anticuerpos anti-HLA antes del trasplante. Esto indica claramente que la inmunización frente a antígenos de HLA representa una barrera para el acceso al trasplante

INTRODUCTION: Anti-HLA antibodies determination in the serum of patients on a waiting list for renal transplant is essential to optimize donor selection as well as for the induction and maintenance immunosuppression scheme, according to immunological risk. These antibodies could be present before transplantation as a result of being exposed to blood transfusions, pregnancies and previous transplants. The objective of the study was to determine immunization against HLA antigens, associated factors and their impact on the waiting list for a renal transplant. METHODS: In this observational retrospective cross sectional study, 254 patients on the waiting list for renal transplant were included. These patients attended the Public Health central laboratory between July 2013 and July 2015. RESULTS: 30% of the 254 studied patients presented anti-HLA antibodies. The most significant sensitizing event was the exposure to a previous transplant (p=<0.05). Multiparous women were in second place, 69% of them presenting positive PRA (panel reactive antibodies) (p=<0.05). Finally 24% of poly transfused patients presented anti-HLA antibodies (p=<0.05). CONCLUSIONS: During the 2 year of the study, 51 patients were transplanted, presenting only one of them anti-HLA antibodies before transplantation. This results clearly indicate that the immunization against HLA represents a barrier for transplantation access
Descritores: Antígeno-1 Associado à Função Linfocitária
Transplante de Rim
Insuficiência Renal Crônica
Histocompatibilidade
Antígenos de Histocompatibilidade
Indicadores e Reagentes
Limites: Humanos
Responsável: AR444.1 - BAN - Biblioteca Argentina de Nefrología Dr. Víctor R. Miatello


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Id: lil-692194
Autor: Vicuña, Lucas; Pardo, Evelyn; Curkovic, Cristóbal; Doger, Remziye; Oyanadel, Claudia; Metz, Claudia; Massardo, Loreto; González, Alfonso; Soza, Andrea.
Título: Galectin-8 binds to LFA-1, blocks its interaction with ICAM-1 and is counteracted by anti-Gal-8 autoantibodies isolated from lupus patients
Fonte: Biol. Res;46(3):275-280, 2013. ilus, graf.
Idioma: en.
Projeto: CONICYT; . FONDECYT.
Resumo: Galectin-8 belongs to a family of mammalian lectins that recognize glycoconjugates present on different cell surface components and modulate a variety of cellular processes. A role of Gal-8 in the immune system has been proposed based on its effects in immune cells, including T and B lymphocytes, as well as the presence of anti-Gal-8 autoantibodies in the prototypic autoimmune disease systemic lupus erythematosus (SLE). We have previously described that Gal-8 induces apoptosis in activated T cells interacting with certain β1 integrins and this effect is counteracted by the anti-Gal-8 autoantibodies. Given that Gal-8 can potentially interact with several glycoproteins, here we analyzed the β2 integrin Lymphocyte Function-Associated Antigen-1 (LFA-1), which is involved in leukocyte cell adhesion and immunological synapses. We show by GST-pull down assays that Gal-8 interacts with LFA-1 and this interaction is inhibited by anti-Gal-8 autoantibodies isolated from SLE patients. In cell adhesion assays, Gal-8 precluded the interaction of LFA-1 with its ligand Intracellular Adhesion Molecule-1 (ICAM-1). These results suggest that Gal-8 can exert immunosuppressive action not only by inducing apoptosis in activated T cells but also by negatively modulating the crucial function of LFA-1 in the immune system, while function-blocking autoantibodies counteract these effects.
Descritores: Galectinas/metabolismo
Molécula 1 de Adesão Intercelular/metabolismo
Lúpus Eritematoso Sistêmico/imunologia
Antígeno-1 Associado à Função Linfocitária/metabolismo
-Anticorpos Monoclonais/imunologia
Anticorpos Monoclonais/metabolismo
Autoanticorpos/imunologia
Autoanticorpos/metabolismo
Adesão Celular
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: lil-646732
Autor: Cavallari, Fransérgio Emílio; Valera, Fabiana Cardoso Pereira; Gallego, Aline Jorge; Malinsky, Rafael Rossell; Küpper, Daniel Salgado; Milanezi, Cristiane; Silva, João Santana da; Tamashiro, Edwin; Anselmo-Lima, Wilma Terezinha.
Título: Expression of RANTES, eotaxin-2, ICAM-1, LFA-1 and CCR-3 in chronic rhinosinusitis patients with nasal polyposis / Expressão de RANTES, eotaxina-2, ICAM-1, LFA-1 e CCR-3 em pacientes com rinossinusite crônica associada à polipose nasossinusal
Fonte: Acta cir. bras;27(9):645-649, Sept. 2012. ilus.
Idioma: en.
Resumo: PURPOSE: To compare gene expression of the chemokines RANTES and eotaxin-2, its receptor, CCR-3, adhesion molecule ICAM-1 and its receptor LFA-1 in eosinophilic polyps and in control normal nasal mucosa. METHODS: Gene expression was quantified by Real Time PCR in polyps (n=35) and in healthy nasal mucosa (n=15). RESULTS: Eosinophilic polyps showed a higher expression of eotaxin-2 and RANTES, but not of CCR-3, ICAM-1 or LFA-1 compared to control nasal mucosa. CONCLUSION: Eosinophilic polyps present greater expression of eotaxin-2 and RANTES, but not of CCR-3, ICAM-1 or LFA-1 compared to control nasal mucosa.

OBJETIVO: Comparar a expressão gênica das quimiocinas RANTES e eotaxina-2, do seu receptor CCR-3, da molécula de adesão ICAM-1 e do seu receptor LFA-1 entre pólipos nasais eosinofílicos (PE) (n=35) e mucosa nasal controle (n=15). MÉTODOS: Quantificou-se a expressão gênica dos mediadores citados pela técnica de PCR em tempo real em PEs e em mucosas de concha média de pacientes sem doenças nasais ou alteração endoscópica. RESULTADOS: Pólipos eosinofílicos apresentam maior expressão de eotaxina-2 e RANTES, mas não de CCR-3, ICAM-1 e LFA-1, quando comparados as mucosas nasais controles. CONCLUSÃO: Pólipos eosinofícios apresentaram maior expressão de eotaxin-2 and RANTES, mas não de CCR-3, ICAM-1 ou LFA-1,comparada à mucosa nasal controle.
Descritores: Pólipos Nasais/metabolismo
Rinite/metabolismo
Sinusite/metabolismo
-Estudos de Casos e Controles
Doença Crônica
/genética
CHEMOKINE CCLABDOMEN/genética
/metabolismo
CHEMOKINE CCLABDOMEN/metabolismo
/genética
CHEMOKINE CCLABORTIFACIENT AGENTS, STEROIDAL/genética
/metabolismo
CHEMOKINE CCLABORTIFACIENT AGENTS, STEROIDAL/metabolismo
Expressão Gênica
Molécula 1 de Adesão Intercelular/genética
Molécula 1 de Adesão Intercelular/metabolismo
Antígeno-1 Associado à Função Linfocitária/genética
Antígeno-1 Associado à Função Linfocitária/metabolismo
Mucosa Nasal
Pólipos Nasais/complicações
Reação em Cadeia da Polimerase
/genética
RECEPTORS, CCRABATTOIRS/genética
/metabolismo
RECEPTORS, CCRABATTOIRS/metabolismo
Rinite/complicações
Sinusite/complicações
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-605688
Autor: Santos, Joel Henrique Teles dos; Torres, Diogo Castelo Branco Alves; Neves, Maria Amelia Batista; Brito, Ana Elita de; Araújo, Rosane Costa Porto de; Machado, Cintia Gonçalves de Faria.
Título: Frequência de imunofenótipos aberrantes em leucemias agudas: análise de 213 casos diagnosticados na Fundação Hemope / Frequency of aberrant immunophenotypes in acute leukemia: analysis of 213 cases diagnosed in Fundação Hemope
Fonte: Rev. bras. anal. clin;43(2):135-137, 2011. tab, graf.
Idioma: pt.
Resumo: As leucemias agudas (LAs) são doenças clonais do tecido hematopoético caracterizadas por proliferação anômala de progenitores das diferentes linhagens. 0 diagnóstico das LAs se baseia em achados citomorfológicos, citoquimicos e imunofenotipicos em células da Medula Óssea (MO) e/ou Sangue Periférico (SP). Cerca de 30 a 50% das LMAs e LLAs, bem caracterizadas pelo imunofenótipo, exibem expressão de antigenos aberrantes e esta situação deve ser distinguida das leucemias bifenotipicas, que tem atualmente critérios de diagnóstico bem definidos. A detecção dos antígenos aberrantes não parece ter implicação prognóstica, mas é uma importante ferramenta para a detecção de doença residual mínima (DRM). 0 objetivo deste trabalho foi relatar a frequência de Fenótipos Aberrantes (FA) em LAs nos pacientes diagnosticados na Fundação Hemope, correlacionar este achado com a idade e identificar os antígenos aberrantes predominantes. 0 estudo contou com 213 pacientes de ambos os sexos e sem restric;:ao de faixa etária ou raça. A imunofenotipagem utilizou amostras de SP e/ou MO, sendo a análise realizada por citometria de fluxo multiparamétrica. Para as LMAs, LLAs BeLLAs T as frequências de FA encontradas foram de 47%, 40% e 52%, respectivamente. Os antígenos aberrantes predominantes foram CD? e CD56 para as LMAs e CD13 e CD33 para as LLAs. A frequência de antígenos aberrantes (45%) e a predominância de antígenos linfocitários T e NK na LMAs e de antígenos mielóides nas LLAs, condizem com a literatura. Por outro lado, o predomínio de FA entre os adultos parece sugerir mais uma caracteristica da amostra, onde esta faixa etária predominou, que uma caracteristica biológica das leucemias analisadas. Finalmente, este estudo, definindo melhor o perfil imunofenotipico de nossos pacientes, possibilita o uso deste conhecimento na avaliação de DRM.

Acute leukemias (AL) are clonal diseases of the hematopoietic tissue characterized by anomalous proliferation of precursors of different lineage. The diagnosis of AL is based on morphological, citochemical and immunophenotypical findings in cells of Bone Marrow (BM) and/or Peripheral Blood (PB). About 30 to 50% of AML and ALL, well-characterized by immunophenotype, display aberrant expression of antigens and this situation should be distinguished from biphenotipic leukemias, which are currently well-defined criteria for diagnosis. The detection of aberrant antigen does not seem to have prognostic implication, but it is an important tool for the detection of minimal residual disease (MRD). The objective of this study was to report the frequency of aberrant phenotypes (AP) at AL in patients diagnosticated by Funda9iio Hemope, correlate this finding with age and identify the predominant aberrant antigens. The study included 213 patients of both genders and without restriction of age or race. The immunophenotyping used samples of PB and/or BM, being the analysis by multiparametric flow cytometry. For AML, B-ALL and T-ALL the frequencies of FA found were 47%, 40% and 52% respectively. The predominant aberrant antigens for AML were CD7 and CD56 and for ALL were CD33 and CD13. The frequency of aberrant antigens (45%) and the predominance of lymphocyte T and NK antigens in AML and myeloid antigens in ALL, matches with the literature. Moreover, the prevalence of AP among adults, who are the predominant age group, seems to be more suggestive of a characteristic of the sample, than a biological feature of leukemias that were analyzed. Finally, this study, defines better the immunophenotypical profile of our patients and allows the use of this knowledge in the evaluation.
Descritores: Citometria de Fluxo
Imunofenotipagem
Antígeno-1 Associado à Função Linfocitária
Leucemia/diagnóstico
-ANTIGENOS CD1ABATTOIRS
ANTIGENOS CDACANTHOSIS NIGRICANS
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto Jovem
Pessoa de Meia-Idade
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-228623
Autor: Tapia, F. J; Caceres-Dittmar, G; Sanchez, M. A; Fernandez, A. E; Convit, J.
Título: The cutaneous lesion in American leishmaniasis: leukocyte subsets, cellular interaction and cytokine production
Fonte: Biol. Res;26(1/2):239-47, 1993. tab, graf.
Idioma: en.
Resumo: Interactions between immunocompetent cells require the participation of T cell antigen receptor (TCR) and the integrin lymphocyte function-associated molecule-1 (LFA-1, CD11a/CD18). These interactions are mediated by interlinking cytokines, which are important in determining the type of immune response. In the present study, we have shown that in American cutaneous leishmaniasis (ACL) lesions, most infiltrating T cells expressed the alpha beta TCR including those selectively migrating to the epidermis. In contrast, gamma delta T cells were abundant in localized (LCL) and scarce in muco-cutaneous (MCL) and diffuse (DCL) cutaneous leishmaniasis, suggesting a role in effective granulomas. There were differences in the expression of LFA-1 alpha and beta subunits, with most cells expressing LFA-1 beta. The ratio LFA-1 beta/LFA-1 alpha was higher in LCL (11.8:1) than in MCL (3.3:1) and DCL (2.4:1). Similar results were observed in Leishmania mexicana-infected C57BL/6 mice. DCL lesions showed a higher proportion of LFA-1 alpha+ cells than MCL and LCL lesions. A reverse-transcriptase polymerase chain reaction (RT-PCR) analysis of the cytokine profiles showed that most T cells present in the MCL and DCL lesions secrete a mixture of Type 1 and Type 2 cytokine patterns, but in DCL granulomas predominate the Type 2 cytokines. In LCL the cytokine patterns show a preponderance of INF gamma over IL-4, and low levels of IL-5 and IL-10, suggesting a Type 1 cytokine profile
Descritores: Leishmaniose Cutânea/imunologia
Linfocinas/biossíntese
Pele/imunologia
Subpopulações de Linfócitos T/imunologia
-Anticorpos Monoclonais
Moléculas de Adesão Celular/biossíntese
Granuloma/imunologia
Leishmaniose Tegumentar Difusa/imunologia
Leishmaniose Mucocutânea/imunologia
Antígeno-1 Associado à Função Linfocitária/biossíntese
Linfocinas/imunologia
Camundongos Endogâmicos C57BL
Reação em Cadeia da Polimerase
Receptores de Antígenos de Linfócitos T/biossíntese
DNA Polimerase Dirigida por RNA
Linfócitos T/imunologia
Limites: Animais
Feminino
Humanos
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-212509
Autor: Eylar, E. H; Molina, Carmen; Báez, Ineabely; Kessler, Matt.
Título: Slow cluster formation of purified human or rhesus T cells requires protein kinasse C and LFA-1
Fonte: P. R. health sci. j;15(1):13-9, mar. 1996. ilus, tab, graf.
Idioma: en.
Resumo: Ab: Homotropic T cell adhesion, as generally studied, consists of a rapid, transient binding process that is measured over a 15-120 min. period. Here we report a slow type of adhesion process occurring with human or rhesus T cells, purified from peripheral blood, that manifests itself by the formation of rounded, multi-layer clusters which may contain hundreds of cells. The maximal number and size of the clusters peak 1-2 days after the addition of phorbol ester, an absolute requirement. The number of clusters formed is proportional to phorbol ester concentration up to 1.25 ng/mL. Phorbol esters such as phorbol myristate acetate (PMA), phorbol dibutyrate (PDB), and 7-octylindolactam (OIL) induced optimal cluster formation at 1-13 ng/mL, levels slightly higher than that required to induce mitogenesis of purified T cells. Phorbol itself and the alpha-form of the ester were inactive. Both cluster formation and mitogenesis (stimulated by Con A or anti-CD3) are completely inhibited by staurosporin at 12.5 ng/mL. Even at 2.5 ng/mL, 74 percent of cluster formation was inhibited, which strongly implies a crucial role for protein kinase C. In the presence of accessory cells, T cell clusters were suppressed. Monoclonal Ab such as anti-CD3, mouse anti-CD3 followed by anti-mouse IgG, anti-CD4, anti-CD4A, anti-CD2, anti-CD8, and anti-CD45 did not induce cluster formation. None were inhibitory or stimulatory in the presence of PMA, except for anti-CD3 which enhanced cluster formation by 26 percent. However, anti-LFA-1 beta-chain (mouse monoclonal) completely blocked cluster formation over the range studied (63-1000 ng/mL) for both human and rhesus cells; rat anti-LFA-1 only blocked human cell adhesion. Anti LFA-1 only partially inhibited T cell mitogenesis. These results show that slow cluster formation shares the LFA-1 and phorbol ester requirements of the rapid adhesion of T cells requiring LFA-1 and ICAM-1. However, cluster occurs at a very low phorbol ester concentration, appears more sensitive to staurosporin inhibition, and is not stimulated via the TCR receptor like the rapid adhesion process. We hypothesize that certain neuronal processes, induced by phorbol ester, and which also show a similar protein kinase C activation time course, may share mechanisms in common with cluster formation
Descritores: Adesão Celular/imunologia
Agregação Celular/imunologia
Teste de Inibição de Aderência Leucocítica
Antígeno-1 Associado à Função Linfocitária/fisiologia
Proteína Quinase C/fisiologia
Linfócitos T/imunologia
-Adesão Celular
Agregação Celular
Ésteres de Forbol/farmacologia
Macaca mulatta
Ativação Linfocitária
Ativação Linfocitária/imunologia
Limites: Humanos
Animais
Ratos
Camundongos
Responsável: BR1.1 - BIREME



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