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Pesquisa : D12.776.395.550.448.100 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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Id: biblio-893662
Autor: Öncel Torun, Zeynep; Torun, Deniz; Baykal, Barış; Öztuna, Ali; Yeşildal, Fatih; Avcu, Ferit.
Título: Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells
Fonte: J. appl. oral sci;25(6):631-640, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Projeto: Scientific and Technological Research Council of Turkey.
Resumo: Abstract Objectives: The primary purpose of this study was to examine the effects of triethylene glycol dimethacrylate (TEGDMA) on odontoclastic differentiation in the dental pulp tissue. Material and Methods: The effects of different TEGDMA dosages on the odontoclastic differentiation capability of dental pulp cells were analyzed in vitro using the following methodologies: i) flow cytometry and tartrate-resistant acid phosphatase (TRAP) staining; ii) apoptotic effects using Annexin V staining; iii) mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor (NF)-kB ligand (RANKL) genes by quantitative Real-time PCR (qRT-PCR); and iv) OPG and RANKL protein expression by enzyme-linked immunosorbent assay (ELISA). Results: TEGDMA caused relatively less odontoclastic differentiation in comparison with the control group; however, odontoclastic differentiation augmented with increasing doses of TEGDMA (p<0.05). The mRNA and protein expression of OPG was lower in TEGDMA treated pulp cells than in the control group (p<0.05). While the mRNA expression of RANKL remained unchanged compared to the control group (p>0.05), its protein expression was higher than the control group (p<0.05). In addition, TEGDMA increased the apoptosis of dental pulp cells dose dependently. Conclusions: TEGDMA reduced the odontoclastic differentiation ability of human dental pulp cells. However, odontoclastic differentiation ratios increased proportionally with the increasing dose of TEGDMA.
Descritores: Polietilenoglicóis/farmacologia
Ácidos Polimetacrílicos/farmacologia
Diferenciação Celular/efeitos dos fármacos
Polpa Dentária/efeitos dos fármacos
Fosfatase Ácida Resistente a Tartarato/efeitos dos fármacos
-Ensaio de Imunoadsorção Enzimática
Receptores de Lipopolissacarídeos/metabolismo
Polpa Dentária/citologia
Ligante RANK/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Citometria de Fluxo
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-842788
Autor: Miyamoto, Maristela; Gouvêa, Aída FTB; Ono, Erika; Succi, Regina Célia M; Pahwa, Savita; Moraes-Pinto, Maria Isabel de.
Título: Immune development in HIV-exposed uninfected children born to HIV-infected women
Fonte: Rev. Inst. Med. Trop. Säo Paulo;59:e30, 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: ABSTRACT Immunological and clinical findings suggestive of some immune dysfunction have been reported among HIV-exposed uninfected (HEU) children and adolescents. Whether these defects are persistent or transitory is still unknown. HEU pediatric population at birth, 12 months, 6-12 years were evaluated in comparison to healthy age-matched HIV-unexposed controls. Plasma levels of LPS, sCD14, cytokines, lymphocyte immunophenotyping and T-cell receptor excision circles (TREC) were assessed. HEU and controls had similar LPS levels, which remained low from birth to 6-12 years; for plasma sCD14, IL-2, IL-6, IL-7, IL-10, IL-12p70, IL-13, IL-17, IFN-γ, TNF-α, G-CSF, GM-CSF and MCP-1, which increased from birth to 12 months and then decreased at 6-12 years; and for TREC/106 PBMC at birth in HEU and controls. By contrast, plasma MIP-1β levels were lower in HEU than in controls (p=0.009) at 12 months, and IL-4 levels were higher in HEU than controls (p=0.04) at 6-12 years. Immune activation was higher in HEU at 12 months and at 6-12 years than controls based on frequencies of CD38+HLA-DR+CD8+T cells (p=0.05) and of CD38+HLA-DR+CD4+T cells (p=0.006). Resting memory and activated mature B cells increased from birth to 6-12 years in both groups. The development of the immune system in vertically HEU individuals is comparable to the general population in most parameters, but subtle or transient differences exist. Their role in influencing clinical incidences in HEU is unknown.
Descritores: Complicações Infecciosas na Gravidez/imunologia
Infecções por HIV/imunologia
Lipopolissacarídeos/sangue
Citocinas/sangue
Contagem de Linfócito CD4
Receptores de Lipopolissacarídeos/sangue
-Valores de Referência
Fatores de Tempo
Biomarcadores/sangue
Estudos de Casos e Controles
Lipopolissacarídeos/imunologia
Citocinas/imunologia
Exposição Materna
Receptores de Lipopolissacarídeos/imunologia
Citometria de Fluxo
Memória Imunológica
Limites: Seres Humanos
Masculino
Feminino
Gravidez
Recém-Nascido
Lactente
Criança
Responsável: BR1.1 - BIREME


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Id: biblio-969346
Autor: Riquelme, José Belarmino; Cazanga, Victoria Matilde; Palma, Cristina Judith; Pérez, Rubén.
Título: Clinical and hematological changes in sheep induced by Escherichia coli lipopolysaccharide / alterações clínicas e hematológicas induzidas pela administração de lipopolisscarídeo de Escherichia coli em ovelhas / Alteraciones clínicas y hematológicas inducidas por la administración de lipopoliscárido de Escherichia coli en ovejas
Fonte: Arq. Ciênc. Vet. Zool. UNIPAR (Online);21(2):47-53, abr.-jun. -2018. graf, tab.
Idioma: en.
Resumo: Organic response to infection is characterized by a systemic reaction known as acute phase response (APR). In order to know the effect of the administration of Escherichia coli lipopolysaccharide (LPS) on physiological, hematological and biochemical variables, 10 sheep weighing 45 ± 5 kg were divided in two groups: Experimental group treated with 3 doses of 1 µg.kg-1 LPS and control group treated with saline solution (SS) at the same frequency as experimental group. Body temperature (BT°), heart rate (HR) and respiratory rate (RR) were monitored. Blood samples for hemogram and enzyme activity for aspartate amino transferase (AST) and gamma glutamyl transferase (GGT) were collected between 1 and 24 h post-LPS. LPS-treated sheep presented mean values of BT (41.2 ± 0.4°C), HR (132 ± 12.3 beats/min) and RR (107.2 ± 25 cycles/min) higher than those observed in control sheep (39.8 ± 0.2°C, 88.8 ± 8.7 beats/min and 53.6 ± 17.1 cycles/min respectively). Between 4 and 8 hours post-injection (hpi) of LPS the leukocyte count was associated with lymphopenia, followed by leukocytosis at 24 hours. No changes were observed in the activity of AST and GGT enzymes. The results characterize APR induced by LPS in sheep, representing a useful model to study cardiovascular, hematological and biochemical responses to infection. (AU)

A resposta orgânica à infecção é caracterizada por uma reação sistêmica conhecida como resposta de fase aguda (RFA). Para conhecer o efeito da administração de lipopolissacárideo (LPS) de Escherichia coli sobre as variáveis fisiológicas, hematológicas e bioquímicas, 10 carneiros pesando 45 ± 5 kg foram divididos em dois grupos: o grupo experimental tratado com três doses de 1 µg.kg -1 de LPS e grupo controle tratado com solução salina (SS) na mesma frequência que o grupo experimental. Temperatura corporal (T°C), frequência cardíaca (FC) e frequência respiratória (FR) foram monitoradas. As amostras de sangue foram tomadas para hemograma e atividade da enzima aspartato aminotransferase (AST) e gama- -glutamiltransferase (GGT) entre uma e 24 h pós-LPS. Ovelhas tratadas com LPS apresentaram valores médios de T°C (41,2 ± 0,4°C), FC (132 ± 12,3 batimentos/min) e FR (107,2 ± 25 ciclos/min) acima dos observados em ovelhas do tratamento controle (39,8 ± 0,2°C, 88,8 ± 8,7 batimentos/min e 53,6 ± 17,1 ciclos/min, respectivamente). Entre 4 e 8 horas após a injeção de LPS, a contagem de leucocitos foi asociado com linfopenia, seguida de leucocitose as 24 horas. Nenhuma mudança na atividade das enzimas AST e GGT foi observada. Os resultados caracterizam uma resposta de fase aguda induzida por LPS em ovelhas, o que representa um modelo útil para estudar os sistemas cardiovascular, hematológico e bioquímico em resposta à infecção.(AU)

La respuesta orgánica a la infección se caracteriza por una reacción sistémica conocida como respuesta de fase aguda (RFA). Para conocer el efecto de la administración de lipopolisacárido (LPS) de Escherichia coli sobre las variables fisiológicas, hematológicas y bioquímicas, 10 ovejas con un peso de 45 ± 5 kg se dividieron en dos grupos: grupo experimental tratado con 3 dosis de 1 µg.kg-1de LPS y grupo control tratado con solución salina (SS) en la misma frecuencia que el grupo experimental. Temperatura corporal (T°C), frecuencia cardíaca (FC) y frecuencia respiratoria (FR) fueron monitoreadas. Se tomaron muestras de sangre para hemograma y actividad enzimática para el aspartato amino transferasa (AST) y gamma glutamil transferasa (GGT) entre 1 y 24 h post-LPS. Las ovejas tratadas con LPS presentaron valores medios de T°C (41.2 ± 0.4°C), FC (132 ± 12.3 latidos / min) y FR (107.2 ± 25 ciclos/min) por encima de los observados en ovinos controles (39.8 ± 0.2°C, 88.8 ± 8.7 latidos/min y 53.6 ± 17.1 ciclos/min respectivamente). Entre las 4 y 8 horas después de la inyección de LPS, el recuento de leucocitos se asoció a linfopenia, seguida de leucocitosis a las 24 horas. No se observaron cambios en la actividad de las enzimas AST y GGT. Los resultados caracterizan una respuesta de fase aguda inducida por LPS en ovinos, representando un modelo útil para estudiar los sistemas cardiovascular, hematológico y bioquímico en respuesta a la infección.(AU)
Descritores: Ovinos/microbiologia
Ovinos/sangue
Receptores de Lipopolissacarídeos/administração & dosagem
Endotoxinas
Escherichia coli/patogenicidade
-Testes Hematológicos/veterinária
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Bertho, Alvaro L
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Id: lil-264228
Autor: Sydow, Farid F. O. von; Santiago, Marta A; Neves-Souza, Patricia C; Cerqueira, Denise I. S; Gouvea, Adriana S; Lavatori, Maryrose F. H; Bertho, Álvaro L; Kubelka, Claire F.
Título: Comparison of dengue infection in human mononuclear leukocytes with mosquito C6/36 and mammalian Vero cells using flow cytometry to detect virus antigen
Fonte: Mem. Inst. Oswaldo Cruz;95(4):483-9, July-Aug. 2000.
Idioma: en.
Resumo: Fluorescent activated cell sorter (FACS) analysis is useful for the detection of cellular surface antigens and intracellular proteins. We used this methodology in order to detect and quantify dengue antigens in highly susceptible cells such as clone C6/36 (Aedes albopictus) and Vero cells (green monkey kidney). Additionally, we analyzed the infection in vitro of human peripheral blood mononuclear leukocytes (PBML). FACS analysis turned out to be a reliable technique to quantify virus growth in traditional cell cultures of C6/36 as well as Vero cells. High rates of infection were achieved with a good statistical correlation between the virus amount used in infection and the percentage of dengue antigen containing cells detected in infected cultures. We also showed that human monocytes (CD14+) are preferred target cells for in vitro dengue infection among PBML. Monocytes were much less susceptible to virus infection than cell lines but they displayed dengue antigens detected by FACS five days after infection. In contrast, lymphocytes showed no differences in their profile for dengue specific immunofluorescence. Without an animal model to reproduce dengue disease, alternative assays have been sought to correlate viral virulence with clinical manifestations and disease severity. Study of in vitro interaction of virus and host cells may highlight this relationship.
Descritores: Vírus da Dengue/imunologia
Dengue/imunologia
Citometria de Fluxo
Leucócitos Mononucleares/imunologia
-Receptores de Lipopolissacarídeos/análise
Receptores de Lipopolissacarídeos/imunologia
Antígenos Virais/análise
Antígenos Virais/imunologia
Linhagem Celular/virologia
Separação Celular
Células Cultivadas
Células Clonais/imunologia
Vírus da Dengue/crescimento & desenvolvimento
Vírus da Dengue/isolamento & purificação
Leucócitos Mononucleares/virologia
Células Vero/citologia
Células Vero/virologia
Limites: Animais
Seres Humanos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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