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Id: biblio-839309
Autor: Xiao, Y; Deng, T; Shang, Z; Wang, D.
Título: Adiponectin inhibits oxidization-induced differentiation of T helper cells through inhibiting costimulatory CD40 and CD80
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;50(6):e6227, 2017. graf.
Idioma: en.
Projeto: Natural Science Foundation of Hubei Province.
Resumo: Adiponectin is a multifunctional adipokine that has several oligomeric forms in the blood stream, which broadly regulates innate and acquired immunity. Therefore, in this study, we aimed to observe the differentiation of T helper (Th) cells and expression of costimulatory signaling molecules affected by adiponectin. The mRNA and protein expression levels of adiponectin and its receptors in oxidized low density lipoprotein cholesterol-treated endothelial cells were assayed by real time PCR and immunofluorescence. The endothelial cells were then treated with adiponectin with or without adipoR1 or adipoR2 siRNA and co-cultured with T lymphocytes. The distribution of Th1, Th2 and Th17 subsets were assayed by flow cytometry. The effects of adiponectin on costimulatory signaling molecules HLA-DR, CD80, CD86 and CD 40 was also assayed by flow cytometry. The results showed that endothelial cells expressed adiponectin and its receptor adipoR1 and adipoR2, but not T-cadherin. Adiponectin suppressed Th1 and Th17 differentiation through adipoR1 receptor, contributed to the inhibition of CD80 and CD40, and inhibited differentiation of Th1 and Th17 by inhibiting antigen presenting action.
Descritores: Adiponectina/metabolismo
Antígeno B7-1/metabolismo
Antígenos CD40/metabolismo
Linfócitos T Auxiliares-Indutores/efeitos dos fármacos
-Adiponectina/genética
Adiponectina/farmacologia
Diferenciação Celular
Células Cultivadas
Células Endoteliais/efeitos dos fármacos
Células Endoteliais/metabolismo
Antígenos HLA-DR/metabolismo
Células Endoteliais da Veia Umbilical Humana/citologia
Lipoproteínas LDL/farmacologia
Receptores de Adiponectina/efeitos dos fármacos
Receptores de Adiponectina/metabolismo
Linfócitos T Auxiliares-Indutores/citologia
Linfócitos T Auxiliares-Indutores/metabolismo
Limites: Humanos
Recém-Nascido
Adulto
Responsável: BR1.1 - BIREME


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Id: lil-173131
Autor: Contreras, María del Carmen; Gallo, Soledad; Salinas, Patricia; Sapunar, Jorge; Sandoval, Lea; Solís, Fresia.
Título: Evaluación de la ELISA IgG, usando un antígeno purificado en el diagnóstico de la hidatidosis humana / Evaluation of ELISA IgG test using a purified antigen in the human hydatidosis diagnosis
Fonte: Bol. chil. parasitol;49(1/2):24-30, ene.-jun. 1994. tab, graf.
Idioma: es.
Resumo: An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of IgG antibodies to purified sheep hydatic cyst fluid antigen in 56 sera of confirmed cases of hydatidosis. The cut-off value was determined using serum samples from 80 healthy persons, employing two serum dilutions (1:100 and 1:500) with two and three standard desviations (SD). This assay was compared with the indirect hemaglutination test (IHAT). The sensitivity of ELISA-IgG was 94,3 for percent, for hepatic cysts and 92,9 for percent, for pulmonary cysts, whereas the values for IHAT were 77,1 and 64,3 for percent, respcetively. According to Mac Nemar test, both thod presented statistical significance (p < 0,05). In order to find out the specificity, additional 70 serum samples from individuals with other parasitoses, such as cysticercosis (30), trichinosis (26) and fascioliasis (14) were also tested, IHAT presented a specificity of 92,7 for percent and for ELISA-IgG the specificity using a cut-off of average + 3 SD was 99,3 and 100,0 por percent with sera dilution of 1:100 and 1:500 respectively when a cut-off of average +2 SD was considered, we found a specificity of 91,3 and 97,3 por percent, for 1:100 and 1:500 dilutions. The use of ELISA-IgG and purified antigen in the diagnosis of human hydatidosis is discussed
Descritores: Equinococose/diagnóstico
Ensaio de Imunoadsorção Enzimática
Testes de Hemaglutinação/métodos
-Antígeno B7-1/imunologia
Antígeno B7-1/isolamento & purificação
Estudos de Casos e Controles
Reações Cruzadas/imunologia
Equinococose/imunologia
Estudo de Avaliação
Helmintíase/diagnóstico
Helmintíase/imunologia
Imunoglobulina G
Sensibilidade e Especificidade
Testes Sorológicos
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Adolescente
Adulto
Pessoa de Meia-Idade
Animais
Tipo de Publ: Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central



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