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Id: lil-716268
Autor: Wan, Y.; Tang, M.H.; Chen, X.C.; Chen, L.J.; Wei, Y.Q.; Wang, Y.S..
Título: Inhibitory effect of liposomal quercetin on acute hepatitis and hepatic fibrosis induced by concanavalin A
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(8):655-661, 08/2014. tab, graf.
Idioma: en.
Projeto: the Scientific Research Foundation for Young Teachers.
Resumo: Immune response plays an important role in the development of hepatic fibrosis. In the present study, we investigated the effects of quercetin on hepatitis and hepatic fibrosis induced by immunological mechanism. In the acute hepatitis model, quercetin (2.5 mg/kg) was injected iv into mice 30 min after concanavalin A (Con A) challenge. Mice were sacrificed 4 or 24 h after Con A injection, and aminotransferase tests and histopathological sections were performed. Treatment with quercetin significantly decreased the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Consistent with this observation, treatment with quercetin markedly attenuated the pathologic changes in the liver. A hepatic fibrosis model was also generated in mice by Con A challenge once a week for 6 consecutive weeks. Mice in the experimental group were treated with daily iv injections of quercetin (0.5 mg/kg). Histopathological analyses revealed that treatment with quercetin markedly decreased collagen deposition, pseudolobuli development, and hepatic stellate cells activation. We also examined the effects of quercetin on the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and transforming growth factor beta (TGF-β) pathways by immunohistochemistry and real-time reverse transcriptase-polymerase chain reaction (RT-PCR). NF-κB and TGF-β production was decreased after treatment with quercetin, indicating that the antifibrotic effect of quercetin is associated with its ability to modulate NF-κB and TGF-β production. These results suggest that quercetin may be an effective therapeutic strategy in the treatment of patients with liver damage and fibrosis.
Descritores: Antioxidantes/administração & dosagem
Hepatite/tratamento farmacológico
Cirrose Hepática/tratamento farmacológico
Quercetina/farmacologia
-Alanina Transaminase/sangue
Aspartato Aminotransferases/sangue
Concanavalina A
Colágeno/análise
Modelos Animais de Doenças
Células Estreladas do Fígado/efeitos dos fármacos
Células Estreladas do Fígado/metabolismo
Lipossomos
Cirrose Hepática/induzido quimicamente
Camundongos Endogâmicos BALB C
Mitógenos
NF-kappa B/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fator de Crescimento Transformador beta/metabolismo
Limites: Animais
Feminino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-665533
Autor: Iglesias, Déborah Pitta Paraíso; Rego, Moacyr Jesus Barreto de Melo; Beltrão, Eduardo Isidoro Carneiro; Sobral, Ana Paula Veras.
Título: CONA and UEA-I ligands during tooth development reveals differences between human and rodents models and shows the beginning of mineral extracelular matrix secretion / Ligandos CONA y UEA-I revelan diferencias entre humanos y en modelos roedores durante el desarrollo dental y muestran el comienzo de la secreción de la matriz mineral extracelular
Fonte: Int. j. morphol;30(3):1100-1105, Sept. 2012. ilus.
Idioma: en.
Resumo: Many of Glycobyological aspects of human development remain still unclear, mainly in oral science, and this could be observed in a lack of literature with few and old papers about this subject. During tooth histo-morphogenesis changes occur in basement membrane composition, expression of signaling molecules and in localization of cell surface components, where glyco components could be involved. In this sense, this work aimed to analyze the ConA ligands commonly founded in glycoproteins cores and UEA-I ligands since fucolization is a Key event in many physiological and pathological processes. Therefore 15 jaws of human fetuses were ethically obtained, histologicaly processed and then submitted to lectin histochemistry with appropriated controls. The results showed that lectins staining increase their intensity during dental development; ConA only starts to recognize glucose/mannose residues on ectomesechymal cells in the crow phase revealing its ligands when the enamel matrix starts to be secreted. Interestingly, Con A ligands were not founded in the basement membrane of the stratum intermedium of the enamel differing from rodents models. The staining pattern of UEA-I was different, starting to be positive in the ectomesenchyma since the bud stage and shown variable expression in cell type and staining intensity, which appeared be directly proportional to the progress of odontogenesis. Thus, this work shows that Con A and UEA-I exhibit a growing staining directly proporcional to ameloblasts and odontoblasts cytodiferenciation and revels some glycan differences between human odontogenesis and rodents models...

Los aspectos glicobiológicos del desarrollo humano siguen siendo poco investigados, sobre todo en odontología, y esto puede ser observado en la literatura por los escasos y antiguos artículos sobre el tema. Durante la histomorfogénesis del diente se producen cambios en la composición de la membrana basal, en la expresión de moléculas de señalización y en la localización de la superficie celular de los componentes, donde los glico componentes podrían estar involucrados. Este trabajo tuvo como objetivo analizar los ligandos de la ConA ya que glucosa/manosa son comúnmente encontrados en núcleos de glicoproteínas y ligandos de la UEA-I debido a fucolización es un evento clave en muchos procesos fisiológicos y patológicos. Fueron obtenidas 15 mandíbulas de fetos humanos, procesadas y tratadas mediante histoquímica de lectinas con controles apropiados. Los resultados mostraron que la intensidad de tinción de las lectinas aumenta durante el desarrollo del diente. ConA sólo comienza a reconocer residuos de glucosa/manosa en células ectomesénquimales en la fase de corona revelando cuando la matriz de esmalte empieza a ser secretada. Curiosamente, ligandos de la ConA no se encontraron en la membrana basal de la capa intermedia del esmalte, difiriendo de los modelos de roedores. El patrón de tinción de la UEA-I fue diferente, empieza a ser positivo en el ectomesenquima desde la etapa de brotación y muestra variable expresión en el tipo de célula y la intensidad de la tinción, que parecía ser directamente proporcional al progreso de la odontogénesis. Por lo tanto, este trabajo demuestra que la Con A y la UEA-I presentan una coloración que crece directamente proporcional a citodiferenciación de los ameloblastos y odontoblastos, y revela algunas diferencias entre el estándar glicano de odontogénesis humanos y los modelos roedores...
Descritores: Carboidratos
Concanavalina A
Odontogênese
Lectinas de Plantas
-Histocitoquímica
Limites: Seres Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: lil-640207
Autor: Conti-Freitas, Luiz C.; Foss-Freitas, Maria C.; Mamede, Rui C. M.; Foss, Norma T..
Título: Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
Fonte: Clinics;67(6):587-590, 2012. tab.
Idioma: en.
Resumo: OBJECTIVE: This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment. METHODS: Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin A and Bacille Calmette-Guerin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured. RESULTS: For non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. The interferon-gamma levels after stimulation with concanavalin A were higher in the late postoperative period cultures than in the preoperative evaluation cultures. Stimulation with Bacille Calmette-Guerin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures. CONCLUSION: Patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferongamma secretion by mononuclear cells. Stimulated cells seem to recover this function during the postoperative period.
Descritores: Carcinoma/sangue
Interferon gama/biossíntese
/biossíntese
INTERLEUKIN-ABDUCENS NERVE/biossíntese
Neoplasias Laríngeas/sangue
Leucócitos Mononucleares/metabolismo
-Estudos de Casos e Controles
Carcinoma/patologia
Concanavalina A/farmacologia
Citocinas/sangue
Interferon gama/sangue
/sangue
INTERLEUKIN-ABDUCENS NERVE/sangue
Neoplasias Laríngeas/patologia
Leucócitos Mononucleares/efeitos dos fármacos
Mycobacterium bovis
Mitógenos/farmacologia
Estadiamento de Neoplasias
Estatísticas não Paramétricas
Limites: Adolescente
Adulto
Criança
Feminino
Seres Humanos
Masculino
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: lil-632361
Autor: Rodríguez-Orozco, Alain R; Ayala-Mata, Francisco; Tinoco-Martínez, Rebeca; Cabrera-Navarro, Lorena.
Título: El nuevo adyuvante de mucosas Cry1Ac potencia la capacidad de células mononucleadas humanas de inhibir el crecimiento bacteriano / The new mucosal adjuvant Cry1Ac increase the capacity of mononuclear cells to stop the bacterial growth up
Fonte: Rev. invest. clín;59(2):161-163, mar.-abr. 2007. tab.
Idioma: es.
Descritores: Proteínas de Bactérias/farmacologia
Toxinas Bacterianas/farmacologia
Endotoxinas/farmacologia
Proteínas Hemolisinas/farmacologia
Leucócitos Mononucleares/efeitos dos fármacos
Viabilidade Microbiana/imunologia
-Amicacina/farmacologia
Antibacterianos/farmacologia
Células Cultivadas/efeitos dos fármacos
Células Cultivadas/imunologia
Concanavalina A/farmacologia
Avaliação Pré-Clínica de Medicamentos
Leucócitos Mononucleares/imunologia
Viabilidade Microbiana/efeitos dos fármacos
Proteus mirabilis/efeitos dos fármacos
Proteus mirabilis/crescimento & desenvolvimento
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/crescimento & desenvolvimento
Proteínas Recombinantes/farmacologia
Salmonella/efeitos dos fármacos
Salmonella/crescimento & desenvolvimento
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/crescimento & desenvolvimento
Limites: Seres Humanos
Tipo de Publ: Estudo Comparativo
Carta
Responsável: MX1.1 - CENIDSP - Centro de Información para Decisiones en Salud Pública


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Id: lil-577244
Autor: Gormaz B., Juan Pablo; Gajardo 0., Pilar; Escobar A., Alejandro; Olavarría L., Christian; Schõnffeldt G., Patricia; Sariego R., Homero; Catalán M., Diego; Aguillón G., Juan Carlos.
Título: Perfil de producción de citoquinas en linfocitos T de sangre periférica de pacientes con poliposis nasal y asma bronquial en respuesta a la estimulación con enterotoxina estafilocócica B / Cytokine production profile in peripheral blood T lymphocytes from patients with nasal poliposis and bronchial asthma in response to staphylococcal enterotoxin B
Fonte: Rev. otorrinolaringol. cir. cabeza cuello;70(3):195-204, dic. 2010. ilus, tab.
Idioma: es.
Projeto: Sociedad Chilena de Otorrinolaringología, Medicina y Cirugía de Cabeza y Cuello. Fondo de Investigación; . Núcleo Milenio en Inmunología e Inmunoterapla.
Resumo: Introducción: La poliposis nasal (PN) se presenta frecuentemente asociada a asma bronquial (AB). La enterotoxina estafilocócica B (SEB) jugaría un papel en su patogenia. No se ha estudiado si el perfil de citoquinas inducido por SEB en linfocitos T (LT) de pacientes con PNyAB difiere del de controles sanos. Objetivo: Comparar el perfil de citoquinas de LT de sangre periférica de pacientes con PN-AByde controles, estimulados con SEB o concanavalina A (ConA). Material y método: Células mononucleares de sangre periférica de 9 pacientes con PN-AB y de 6 controles se estimularon con SEB o ConA. El porcentaje LT CD4+ productores de interferón (IFN)-y, interleuquina (IL) IL-4, IL-5, IL-17 e IL-21 se determinó mediante citometrfa de flujo. Resultados: El grupo PN-AB presentó un menor porcentaje de LT productores de IL-5 que los controles al estimularse con SEB y con ConA. No hubo diferencia en las otras citoquinas estudiadas. Discusión: Nuestros resultados en sangre periférica difieren de lo descrito en tejido de pólipos nasales. Conclusión: Se sugiere que la respuesta inflamatoria de la PN se originaría localmente ya que los LT de sangre de pacientes con PN-AB no muestran una polarización hacia perfiles proinflamatorios con los estímulos utilizados.

Introduction: Nasal poliposis (NP) is frequently associated with bronchial asthma (BA) and its pathogenesis is still unknown. Staphylococcal enterotoxin B (SEB) has been implicated in the development of NP, however if the SEB-induced cytoklne profile of peripheral blood T lymphocytes (TL) of PN-BA patients differs from that of normal controls has not been studied. Aim: To compare the cytoklne profile of CD4+ TL from NP-BA and controls stimulated with SEB or concanavalin A (ConA). Material and method: Peripheral blood mononuclear cells from 9 NP-BA patients and from 6 controls were stimulated with SEB or ConA. The percentage of interferon (IFN)-y, interleukin {II) 11-4,11-5,11-17, and 11-21 producing TL was analyzed by flow cytometry Results: The percentage of SEB and ConA stimulated CD4+ IL-5-producing TLs was lower in the NP-BA group compared to the control group. There were no differences in the other cytokine-producing populations. Discussion: Unlike what is described in nasal polyp tissue, our findings show a diminished production of IL-5 by peripheral TL from the NP-AB group. Conclusion: A local sinonasal origin of the chronic inflammation is suggested since peripheral blood TL of NP-BA patients do not show a pro-inflammatory polarization with the tested stimuli.
Descritores: Asma/imunologia
Citocinas/sangue
Enterotoxinas/farmacologia
/fisiologia
LINFOCITOS T CDABBREVIATIONS AS TOPIC-POSITIVOS/fisiologia
Pólipos Nasais/imunologia
-Asma/sangue
Estudos de Casos e Controles
Técnicas de Cultura
Concanavalina A/farmacologia
Citometria de Fluxo
Ativação Linfocitária
Linfócitos T Auxiliares-Indutores/fisiologia
Pólipos Nasais/sangue
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Responsável: CL1.1 - Biblioteca Central


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Id: lil-492884
Autor: Alves, R; Silva, D. A. O; Fernandes, J. F. C; Almeida, K. C; Ynoue, L. H; Bernardes, C. T. V; Moreira, P. F. S; Gennari-Cardoso, M. L; Sung, S. J; Taketomi, E. A.
Título: Humoral and cellular immune responses to Blomia tropicalis and concanavalin A-binding fractions in atopic patients
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;41(9):773-781, Sept. 2008. graf, tab.
Idioma: en.
Projeto: CAPES; . CNPq; . FAPEMIG.
Resumo: Blomia tropicalis, Dermatophagoides pteronyssinus and D. farinae are prevalent house dust mites. Concanavalin A-binding components derived from B. tropicalis (Bt-ConA extract) are highly immunogenic in allergic diseases. The aim of the present study was to evaluate the humoral and cellular immune responses to B. tropicalis in mite-sensitized patients. A total of 137 patients with allergic rhinitis with/without asthma and 109 non-atopic subjects were selected and analyzed by the skin prick test, and for total serum IgE and specific IgE levels to both Bt-total and Bt-ConA extracts, their proliferative response and cytokine (IFN-ã and IL-5) production by peripheral blood mononuclear cells (PBMC) stimulated with both extracts. Skin prick test showed that 70 percent of the patients were sensitized to Bt (Bt+) and similar levels of specific IgE to Bt-total and Bt-ConA extracts were demonstrable in Bt+ patients. Significant PBMC proliferation was observed in response to Bt-total extract in Bt+, but not in Bt- patients and non-atopic subjects (P < 0.001). Bt-ConA extract induced increased proliferative responses in all patient groups compared to medium alone (P < 0.05), but these responses were significantly decreased in the presence of the mannopyranoside ConA inhibitor (P < 0.05). Significant IFN-ã production was observed after Bt-ConA stimulation of Bt+ patients (P < 0.05), while Bt-total extract had no effect. IL-5 production was consistently detected in Bt+ patients after allergen-specific stimulation or with no stimulus, indicating that PBMC from allergic patients are prone to produce Th2 profile cytokines, spontaneously or inductively by allergen restimulation. These data showed that ConA-binding components isolated from B. tropicalis may contain relevant antigens that are involved in both humoral and cellular immune responses. However, without an additional purification procedure to eliminate the residual contamination with...
Descritores: Alérgenos/administração & dosagem
Antígenos de Dermatophagoides/administração & dosagem
Concanavalina A/administração & dosagem
Mitógenos/administração & dosagem
Rinite Alérgica Perene/imunologia
-Alérgenos/imunologia
Antígenos de Dermatophagoides/imunologia
Estudos de Casos e Controles
Proliferação Celular
Concanavalina A/imunologia
Dessensibilização Imunológica
Imunoglobulina E/sangue
Imunoglobulina E/imunologia
Interferon gama/biossíntese
/biossíntese
INTERLEUKIN-ABDOMEN/biossíntese
Leucócitos Mononucleares/imunologia
Ácaros/imunologia
Mitógenos/imunologia
Rinite Alérgica Perene/sangue
Limites: Adulto
Animais
Feminino
Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-456804
Autor: Simioni, P. U; Costa, E. H; Tamashiro, W. M. S. C.
Título: Aging reduces the primary humoral response and the in vitro cytokine production in mice
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;40(8):1111-1120, Aug. 2007. graf.
Idioma: en.
Projeto: FAPESP; . FAPESP fellowships.
Resumo: Aging is accompanied by a decrease in several physiological functions that make older individuals less responsive to environmental challenges. In the present study, we analyzed the immune response of female BALB/c mice (N = 6) of different ages (from 2 to 96 weeks) and identified significant age-related alterations. Immunization with hapten-protein (trinitrophenyl-bovine serum albumin) conjugates resulted in lower antibody levels in the primary and secondary responses of old mice (72 weeks old). Moreover, young mice (2, 16, and 32 weeks old) maintained specific antibodies in their sera for longer periods after primary immunization than did old mice. However, a secondary challenge efficiently induced memory in old mice, as shown by the increased antibody levels in their sera. The number of CD4+ and CD8+ T cells in the spleen increased until 8 weeks of age but there was no change in the CD4+/CD8+ ratio with aging. Splenic T cells from old mice that had or had not been immunized were less responsive to concanavalin-A and showed reduced cytokine production compared to young mice (IL-2: 57-127 vs 367-1104 pg/mL, IFN-g: 2344-12,836 vs 752-23,106 pg/mL and IL-10: 393-2172 vs 105-2869 pg/mL in old and young mice, respectively). These data suggest that there are significant changes in the organization of the immune system throughout life. However, the relevance of these alterations for the functioning of the immune system is unknown.
Descritores: Envelhecimento/imunologia
Citocinas/biossíntese
Haptenos/imunologia
Baço/imunologia
Subpopulações de Linfócitos T/imunologia
-CDABBREVIATIONS AS TOPIC-CDABDOMINAL NEOPLASMS RATIO
Proliferação Celular/efeitos dos fármacos
Concanavalina A/farmacologia
Imunidade Celular/imunologia
Camundongos Endogâmicos BALB C
Mitógenos/farmacologia
Baço/citologia
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-437831
Autor: Almeida, K. C; Silva, D. A. O; Gennari-Cardoso, M. L; Cunha-Júnior, J. P; Alves, R; Ynoue, L. H; Resende, R. O; Sung, S. J; Taketomi, E. A.
Título: Responses of IgE, IgG1, and IgG4 to concanavalin A-binding Blomia tropicalis antigens in allergic patients
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;39(11):1445-1454, Nov. 2006. tab, graf.
Idioma: en.
Resumo: Blomia tropicalis (Bt) and Dermatophagoides pteronyssinus (Dp) are the prevalent house dust mites in tropical countries and are associated with allergic diseases. Glycosylated antigens are highly immunogenic and involved in different pathologies. We evaluated the presence of IgE, IgG1, and IgG4 to concanavalin A-binding antigens (Bt-Con-A) isolated from Bt-total extract in sera of allergic and non-allergic subjects. Bt-total and Bt-Con-A extracts were evaluated by SDS-PAGE and ELISA for reacting with IgE, IgG1, and IgG4 in sera of 121 patients with allergic rhinitis and 36 non-allergic individuals. All subjects were skin prick tested with Bt-total extract and inhibition tests were performed for IgE, IgG1, and IgG4 using both extracts (Bt-total and Bt-Con-A). Skin prick test showed that 58 percent of the patients were sensitized to Bt (Bt+), with 52 percent reactive to both mites (Bt and Dp) and 6 percent to Bt only. A broad spectrum of proteins (14-152 kDa) was visualized in Bt-total and components >27 kDa for the Bt-Con-A extract. ELISA showed a similar profile of IgE, IgG1 and IgG4 levels in response to Bt-total and Bt-Con-A extracts in different groups, although Bt+ patients showed a lower IgG4 reactivity to Bt-Con-A extract. Specific IgG1 levels were higher in Bt+ patients than in control subjects, and IgG4 levels showed no significant difference among groups. ELISA inhibition showed a partial IgE and total IgG1 and IgG4 cross-reactivity with Dp extract for Bt-total and Bt-Con-A extracts. We conclude that Con-A-binding components isolated from Bt constitute major allergens and are involved in both allergen sensitization (IgE response) and homeostasis maintenance (IgG1 and IgG4 responses).
Descritores: Alérgenos/imunologia
Antígenos de Dermatophagoides/imunologia
Autoanticorpos/imunologia
Concanavalina A/farmacologia
Imunoglobulina E/imunologia
Imunoglobulina G/imunologia
Rinite Alérgica Perene/imunologia
-Especificidade de Anticorpos
Estudos de Casos e Controles
Reações Cruzadas
Eletroforese em Gel de Poliacrilamida
Ensaio de Imunoadsorção Enzimática
Índice de Gravidade de Doença
Limites: Seres Humanos
Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-391428
Autor: Saavedra Hernández, Elsa; Pérez Santiago, Alma; Pérez Campos, Eduardo; Cordoba Alba, Félix.
Título: Efecto de la concanavalina A en el cultivo del fitopatógeno: ustilago maydis / Effect of concanavalin A in the culture of the phytopatogen: ustilago maydis
Fonte: Interciencia;28(5):276-280, mayo 2003. ilus, graf.
Idioma: es.
Resumo: El efecto de la adición de la lectina Concanavalina A al cultivo del hongo Ustilago maydis es examinado para contribuir al entendimiento de los mecanismos de reconocimiento e infección entre hongos y plantas. La cinética de crecimiento del hongo en cultivo mostró que la adición de Con A tiene un discreto efecto activador sobre las basidiosporas a partir de la 9h de incubación. Por otra parte, la unión de Con A a las basidiosporas causó diversos cambios morfológicos, agregación, múltiples ramificaciones y una incremantada capacidad de gemación. La unión de Con A a las basidiosporas fue evidencia utilizando un conjugado fluorescente de la lectina (Alexa-flúor), observándose mayor intensidad de fluorescencia en las puntas y zonas de gemación de las basidiosporas, lo que sugiere la distribución heterógena de estructuras sacarídicas (receptores) sobre la superficie de la pared celular del hongo durante el crecimiento. Los efectos de la adición de Con A en el cultivo del hongo se inhiben al incubar previamente la lectina con &-manopiranosa.
Descritores: Concanavalina A
Meios de Cultura
Lectinas
Ustilago maydis
Tipo de Publ: Revisão
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Meira, D. A
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Id: lil-355752
Autor: Pelizon, A. C; Kaneno, R; Soares, A. M. V. C; Meira, D. A; Sartori, A.
Título: Down-modulation of lymphoproliferation and interferon-gamma production by beta-glucan derived from Saccharomyces cerevisiae
Fonte: Mem. Inst. Oswaldo Cruz;98(8):1083-1087, Dec. 2003. graf.
Idioma: en.
Resumo: Beta-glucan, one of the major cell wall components of Saccharomyces cerevisiae, has been found to enhance immune functions. This study investigated in vivo and in vitro effects of beta-glucan on lymphoproliferation and interferon-gamma (IFN-gamma) production by splenic cells from C57BL/6 female mice. All experiments were performed with particulate beta-glucan derived from S. cerevisiae. Data demonstrated that both, i.p administration of particulate beta-glucan (20 or 100 µg/animal) and in vitro stimulation of splenic cells (20 or 100 µg/ml of culture) decreased lymphoproliferation and IFN-gamma production induced by concanavalin A. These results suggest that beta-glucan can trigger a down-modulatory effect regulating a deleterious immune system hyperactivity in the presence of a strong stimulus.
Descritores: Adjuvantes Imunológicos
Glucanos
Interferon gama
Ativação Linfocitária
Saccharomyces cerevisiae
Baço
-Concanavalina A
Glucanos
Interferon gama
Ativação Linfocitária
Camundongos Endogâmicos C57BL
Baço
Limites: Animais
Feminino
Camundongos
Responsável: BR1.1 - BIREME



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