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Id: lil-788966
Autor: Azizoglu, Ugur; Ayvaz, Abdurrahman; Yılmaz, Semih; Karabörklü, Salih; Temizgul, Rıdvan.
Título: Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
Fonte: Braz. j. microbiol;47(3):597-602, July-Sept. 2016. graf.
Idioma: en.
Projeto: Erciyes University; . Bilim, Sanayi ve Teknoloji Bakanlığı.
Resumo: ABSTRACT In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl β-D-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 µg g-1 caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.
Descritores: Bacillus thuringiensis/fisiologia
Proteínas de Bactérias/genética
Expressão Gênica
Controle de Insetos
Endotoxinas/genética
Proteínas Hemolisinas/genética
-Bacillus thuringiensis/ultraestrutura
Proteínas de Bactérias/metabolismo
Proteínas de Bactérias/toxicidade
Controle de Insetos/métodos
Clonagem Molecular
Endotoxinas/metabolismo
Endotoxinas/toxicidade
Proteínas Hemolisinas/metabolismo
Proteínas Hemolisinas/toxicidade
Inseticidas
Larva
Mariposas/efeitos dos fármacos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-618190
Autor: Thangaraj, S; Bragadeeswaran, S.
Título: Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India
Fonte: J. venom. anim. toxins incl. trop. dis;18(1):53-61, 2012. ilus.
Idioma: en.
Resumo: Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodactyla gigantea, collected from the Mandapam coast, are characterized biomedically and pharmacologically. The crude protein was obtained by using methanol and aqueous extracts. The respective protein contents of S. mertensii and S. gigantea were found to be 2.10 µg/mL and 1.87 µg/mL. The methanol and aqueous extracts of S. mertensii and S. gigantea yielded six and nine bands by SDS-PAGE on 12 percent gel. In the hemolytic assay, both extracts exhibited hemolytic effect on chicken, goat, cow and human erythrocytes ('A', 'B' and 'O'). The neurotoxic effects of these crude extracts were determined in vivo using the sea shore crab Ocypode macrocera and mortality was observed. The mouse bioassay for lethality was performed on male albino mice. The crude extract of S. mertensii showed higher lethality (58 seconds at 1 mL-dose) than that of S. gigantea (2 minutes and 10 seconds at 0.75 mL-dose). The analgesic activity test was also carried out on albino mice by Eddy's hot plate and tail-flick methods. The extracts showed moderate analgesic effect by both hot-plate and tail-flick methods. These characteristics emphasize the need for the isolation and molecular characterization of new active toxins in S. mertensii and S. gigantea.(AU)
Descritores: Anêmonas-do-Mar/química
Antivenenos
Venenos de Cnidários/toxicidade
Neurotoxinas/química
-Bioensaio/métodos
Proteínas Hemolisinas/isolamento & purificação
Proteínas Hemolisinas/efeitos dos fármacos
Analgésicos/farmacologia
Limites: Animais
Masculino
Ratos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-911518
Autor: Lopes, E. S; Cardoso, W. M; Nishi, D. M; Horn, R. V; Albuquerque, Á. H; Lima, S. V. G; Beleza, A. J. F; Gaio, F. C; Carmo, C. C; Pascoal Filho, M. N; Teixeira, R. S. C.
Título: Serogroup identification, phenotypic detection of hemolysis and extended spectrum beta-lactamases of Escherichia coli isolated from psittacine of illegal wildlife trade in Fortaleza, Brazil / Identificação de sorogrupo e detecção fenotípica de betalactamase de espectro estendido e produção de hemolisina em Escherichia coli isoladas de psitacídeos do tráfico de animais silvestres em Fortaleza-CE
Fonte: Arq. bras. med. vet. zootec. (Online);70(3):823-829, maio-jun. 2018. tab, graf.
Idioma: en.
Resumo: This study aimed to identify serogroups of Escherichia coli important for human health in isolates from psittacine of illegal wildlife trade in Ceará State. In addition, hemolysis and production of Extended Spectrum Beta-Lactamases (ESBL) was assessed in the isolates. A total of 78 E. coli strains isolated from different Psittaciformes species from a wildlife rehabilitation center in Fortaleza, Brazil. The isolates used in this study were previously identified and stored. Serogroup identification was performed using polyvalent sera for EPEC (O55, O111, O119, O114, O125, O86, O126, O127, O128), EIEC (O136, O124) and EHEC (O157). ESBL detection was performed with double disk synergy method. For hemolysis detection, isolates were inoculated in blood agar base enriched with ovine blood. Only 31 (39.7%) isolates were seropositive and the most frequent were O127, O114, O128 and O111. There was no agglutination for serogroups O55, O124, O136 or O157. Considering both seropositive and seronegative isolates, 9 (11.5%) and 35 (44.9%) presented hemolysis and ESBL production, respectively. In conclusion, the investigated psittacine from illegal wildlife trade hosted ESBL-producing E. coli strains and some belong to important serogroups often linked to severe human infections.(AU)

Este trabalho teve como objetivo identificar sorogrupos de E. coli importantes para a saúde humana, oriundos de psitacídeos provenientes do tráfico no estado do Ceará, assim como detectar atividade hemolítica e produção de betalactamase de espectro estendido (ESBL). Foram testadas 78 cepas de Escherichia coli provenientes de psitaciformes do Centro de Triagem de Animais Silvestres, Fortaleza, CE. Para a identificação dos sorogrupos, utilizaram-se soros polivalentes EPEC (O55, O111, O119, O114, O125, O86, O126, O127, O128), EIEC (O136, O124) e EHEC (O157). Para detecção de ESBL, as cepas foram submetidas ao método de aproximação de disco e, para a detecção de hemolisina, foram plaqueadas em ágar sangue base enriquecido com sangue de carneiro. No geral, 31 (39,7%) das amostras foram soropositivas. Os sorogrupos mais frequentemente detectados foram O127, O114, O128 e O111. Não houve positividade para os sorogrupos O55, O124, O136 e O157. Considerando-se as amostras sororreagentes e não sororreagentes, observou-se que nove (11,5%) e 35 (44,9%) cepas de E. coli apresentaram produção de hemolisinas e de ESBL, respectivamente. Em conclusão, constatou-se que psitacídeos provenientes do tráfico de animais silvestres albergam cepas de E. coli produtoras de ESBL e providas de importantes sorogrupos implicados em graves infecções humanas.(AU)
Descritores: beta-Lactamases
Escherichia coli/isolamento & purificação
Papagaios/microbiologia
-Proteínas Hemolisinas/análise
Sorogrupo
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-762924
Autor: Beltrame, C. O.; Côrtes, M. F.; Bandeira, P. T.; Figueiredo, A. M. S..
Título: Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(12):1071-1076, Dec. 2015. tab, graf.
Idioma: en.
Resumo: Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in the RNeasy column. This simple pre-kit procedure yields high-quality total RNA from planktonic and sessile cells of S. aureus.
Descritores: Técnicas Bacteriológicas/normas
Biofilmes/crescimento & desenvolvimento
RNA Bacteriano/isolamento & purificação
Staphylococcus aureus/genética
-Técnicas Bacteriológicas/métodos
Eletroforese em Gel de Ágar
Proteínas Hemolisinas/metabolismo
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Controle de Qualidade
Reação em Cadeia da Polimerase em Tempo Real
Transcrição Reversa
Staphylococcus aureus/fisiologia
Responsável: BR1.1 - BIREME


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Id: lil-759358
Autor: ELLEPOLA, Arjuna Nishantha; KHAJAH, Rana; JAYATILAKE, Sumedha; SAMARANAYAKE, Lakshman; SHARMA, Prem; KHAN, Zia.
Título: Impact of brief exposure to antifungal agents on the post-antifungal effect and hemolysin activity of oral Candida albicans
Fonte: J. appl. oral sci;23(4):412-418, July-Aug. 2015. tab.
Idioma: en.
Projeto: Kuwait University Research.
Resumo: AbstractPost-antifungal effect (PAFE) of Candida and its production of hemolysin are determinants of candidal pathogenicity. Candida albicans is the foremost aetiological agent of oral candidosis, which can be treated with polyene, azole, and echinocandin antifungals. However, once administered, the intraoral concentrations of these drugs tend to be subtherapeutic and transient due to the diluent effect of saliva and cleansing effect of the oral musculature. Hence, intra-orally, Candidamay undergo a brief exposure to antifungal drugs.Objective Therefore, the PAFE and hemolysin production of oral C. albicans isolates following brief exposure to sublethal concentrations of the foregoing antifungals were evaluated.Material and Methods A total of 50 C. albicans oral isolates obtained from smokers, diabetics, asthmatics using steroid inhalers, partial denture wearers and healthy individuals were exposed to sublethal concentrations of nystatin, amphotericin B, caspofungin, ketoconazole and fluconazole for 60 min. Thereafter, the drugs were removed and the PAFE and hemolysin production were determined by previously described turbidometric and plate assays, respectively.Results Nystatin, amphotericin B, caspofungin and ketoconazole induced mean PAFE (hours) of 2.2, 2.18, 2.2 and 0.62, respectively. Fluconazole failed to produce a PAFE. Hemolysin production of these isolates was suppressed with a percentage reduction of 12.27, 13.47, 13.33, 8.53 and 4.93 following exposure to nystatin, amphotericin B, caspofungin, ketoconazole and fluconazole, respectively.Conclusions Brief exposure to sublethal concentrations of antifungal drugs appears to exert an antifungal effect by interfering with the growth as well as hemolysin production of C. albicans.
Descritores: Antifúngicos/farmacologia
Candida albicans/efeitos dos fármacos
Candida albicans/isolamento & purificação
Farmacorresistência Fúngica/efeitos dos fármacos
Proteínas Hemolisinas/efeitos dos fármacos
-Anfotericina B/farmacologia
Estudos de Casos e Controles
Contagem de Colônia Microbiana
Candida albicans/metabolismo
Equinocandinas/farmacologia
Fluconazol/farmacologia
Proteínas Hemolisinas/metabolismo
Cetoconazol/farmacologia
Testes de Sensibilidade Microbiana
Nistatina/farmacologia
Estatísticas não Paramétricas
Fatores de Tempo
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-756568
Autor: Landim, Cinthia Silvestre; Gomes, Francisco Carlos Almeida; Zeza, Bernardete Martin; Mendrone-Júnior, Alfredo; Dinardo, Carla Luana.
Título: Prophylactic strategies for acute hemolysis secondary to plasma-incompatible platelet transfusions: correlation between qualitative hemolysin test and isohemagglutinin titration
Fonte: Rev. bras. hematol. hemoter;37(4):217-222, July-Aug. 2015. tab, ilus.
Idioma: en.
Resumo: Brazilian legislation has recently suggested the use of the qualitative hemolysin test instead of isohemagglutinin titers as prophylaxis for acute hemolysis related to plasma-incompatible platelet transfusions. The efficacy of this test in preventing hemolytic reactions has never been evaluated while isohemagglutinin titers have been extensively studied. The main objective of this study was to evaluate the correlation between the results of these two tests. The impact of each type of prophylaxis on the platelet inventory management and the ability of the qualitative hemolysin test to prevent red cell sensitization after the transfusion of incompatible units were also studied.METHODS: A total of 246 donor blood samples were evaluated using both isohemagglutinin titers and the qualitative hemolysin test, and the results were statistically compared. Subsequently, 600 platelet units were tested using the hemolysin assay and the percentage of units unsuitable for transfusion was compared to historical data using isohemagglutinin titers (cut-off: 100). Moreover, ten patients who received units with minor ABO incompatibilities that were negative for hemolysis according to the qualitative hemolysin test were evaluated regarding the development of hemolysis and red cell sensitization (anti-A or anti-B).RESULTS: Isohemagglutinin titration and the results of qualitative hemolysin test did not correlate. The routine implementation of the qualitative hemolysin test significantly increased the percentage of platelet units found unsuitable for transfusions (15-65%; p-value <0.001)...
Descritores: Sistema do Grupo Sanguíneo ABO
Incompatibilidade de Grupos Sanguíneos
Proteínas Hemolisinas
Hemólise
Transfusão de Plaquetas
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-723107
Autor: Navidinia, Masoumeh; Peerayeh, Shahin Najar; Fallah, Fatemeh; Bakhshi, Bita; Sajadinia, Raheleh Sadat.
Título: Phylogenetic grouping and pathotypic comparison of urine and fecal Escherichia coli isolates from children with urinary tract infection
Fonte: Braz. j. microbiol;45(2):509-514, Apr.-June 2014. tab.
Idioma: en.
Resumo: The aim of this study was to investigate the phylogenetic background and to assess hlyD (involved in the secretion of haemolysin A) and intll (encoding a class 1 integrase) in Escherichia coli isolates derived from urinary and fecal specimens. A total of 200 E. coli isolates was collected from patients presenting with urinary tract infection (UTI) during September 2009 to September 2010 and screened for hlyD and intll genes by polymerase chain reaction (PCR). Phylogenetic analysis showed that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D) and that uropathogenic E. coli (UPEC) isolates mainly belong to groups B2 (54%) and D (34%) whereas group A (44%) and D (26%) are predominant among commensal E. coli isolates. In this study, hlyD was present in 26% of UPEC and 2% of commensal E. coli isolates. However, hemolytic activity was detected for 42% of UPEC and 6% of commensal E. coli isolates (p < 0.05). intll gene was more frequently expressed in UPEC (24%) in comparison with commensal E. coli isolates (12%). Resistance to aztreonam, co-trimoxazole and cefpodoxime were frequently found among UPEC isolates whereas commensal E. coli isolates were commonly resistant to co-trimoxazole, nalidixic acid and cefotaxime. Concluding, a considerable difference between UPEC and commensal E. coli isolates was observed regarding their phylogenetic groups, presence of class 1 integron and hlyD gene, hemolysin activity and resistance pattern. The detection of class 1 integrons and hlyD gene was higher among UPEC compared with commensal E. coli isolates. These findings may contribute for a better understanding of the factors involved in the pathogenesis of UPEC.
Descritores: Infecções por Escherichia coli/microbiologia
Escherichia coli/classificação
Fezes/microbiologia
Variação Genética
Filogenia
Infecções Urinárias/microbiologia
Urina/microbiologia
-Antibacterianos/farmacologia
Análise por Conglomerados
Farmacorresistência Bacteriana
Proteínas de Escherichia coli/genética
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Genótipo
Proteínas Hemolisinas/genética
Integrases/genética
Proteínas de Membrana Transportadoras/genética
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Limites: Criança
Pré-Escolar
Feminino
Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-714779
Autor: Salles, Hévila Oliveira; Braga, Ana Carolina Linhares; do Nascimento, Maria Thayana dos Santos Canuto; Sousa, Ana Márjory Paiva; Lima, Adriano Rodrigues; Vieira, Luiz da Silva; Cavalcante, Antônio Cézar Rocha; do Egito, Antonio Silvio; Andrade, Lúcia Betânia da Silva.
Título: Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus / Lectina, hemolisina e inibidores de protease em frações de sementes com atividade ovicida contra Haemonchus contortus
Fonte: Rev. bras. parasitol. vet;23(2):136-143, 06/2014. tab, graf.
Idioma: en.
Resumo: Bioactive molecules of plant species are promising alternatives for the chemical control of gastrointestinal nematodes in ruminants. Extracts of native and exotic seed species from Brazil's semi-arid region were tested in vitro in an egg hatch assay and the bioactivity of their proteins was investigated. Each seed species was subjected to three extractions with three types of solvents. All the seeds showed ovicidal activity, which varied according to the solvents. Higher ovicidal activity was found in the molecule fractions of low molecular weight (<12 kDa) for Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera and Ricinus communis (P<0.05, Bonferroni test). The two fractions of Crotalaria spectabilis showed the same ovicidal activity (P>0.05, Bonferroni test). Hemagglutinating activity was detected in the fractions of C. spectabilis and M. oleifera fractions, hemolysin activity in the A. lebbeck and M. oleifera fractions, serine protease inhibitory activity in the A. lebbeck, I. asarifolia, J. curcas, M. oleifera and R. communis fractions, cysteine protease inhibitor activity in the M. oleifera fraction, and no protein activity in the L. ferrea fraction. The results of this work reveal new plant species with a potential for use in controlling nematode parasites in goats, thus opening a new field of research involving plant protein molecules with ovicidal properties.

Moléculas bioativas de espécies vegetais são alternativas promissoras ao controle químico dos nematoides gastrintestinais em ruminantes. Extratos de sementes de espécies nativas e exóticas do Semiárido Brasileiro foram testados in vitro em ensaio de eclosão de ovos e investigada a natureza proteica da bioatividade. Três extrações com três solventes foram feitas para cada semente estudada. Todas as sementes apresentaram atividade ovicida, variando com o solvente utilizado. Maior taxa de inibição da eclosão concentrou-se nas frações de moléculas de baixa massa molecular (<12 kDa) para Albizia lebbeck, Ipomoea asarifolia, Jatropha curcas, Libidibia ferrea, Moringa oleifera e Ricinus communis (P<0,05, teste de Bonferroni). Crotalaria spectabilis mostrou atividade nas duas frações, sem diferença entre elas (P>0,05, teste de Bonferroni). Observou-se atividade hemaglutinante nas frações de C. spectabilis e M. oleifera, de hemolisina em A. lebbeck e M. oleifera, de atividade inibidora de protease da serina em A. lebbeck, I. asarifolia, J. curcas, M. oleifera e R. communis, de atividade inibidora de protease da cisteína em M. oleifera e nenhuma atividade proteica na fração de L. ferrea. Os resultados revelaram novas espécies botânicas com potencial de controle de nematoides em caprinos e um novo campo de pesquisa, o estudo de moléculas de origem proteica com atividade ovicida.
Descritores: Haemonchus/efeitos dos fármacos
Proteínas Hemolisinas/farmacologia
Lectinas/farmacologia
Óvulo/efeitos dos fármacos
Extratos Vegetais/farmacologia
Inibidores de Proteases/farmacologia
Sementes
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-705260
Autor: Reis, Andre L.S.; Montanhini, Maike T.M.; Bittencourt, Juliana V.M.; Destro, Maria T.; Bersot, Luciano S..
Título: Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil
Fonte: Braz. j. microbiol;44(4):1195-1198, Oct.-Dec. 2013. ilus, tab.
Idioma: en.
Projeto: CNPq.
Resumo: Bacillus cereusis an ubiquitous, spore-forming bacteria that can survive pasteurization and the majority of the heating processes used in the dairy industry. Besides, it is a pathogen responsible for different types of food poisoning. One type of foodborne disease caused by B.cereusis the diarrheal syndrome, which is caused by the ingestion of vegetative cells producing toxins in the small intestine. One virulence factor for the diarrheal syndrome is the toxin hemolysin BL (HBL), a three-component protein formed by the L1, L2 and B components. In order to evaluate the presence of diarrheal strains isolated from milk and dairy products, 63 B. cereus isolates were obtained from 260 samples of UHT milk, pasteurized milk and powdered milk, sold in commercial establishments and from different brands. The isolates were subjected to the Polymerase Chain Reaction (PCR) for the detection of the encoding genes for the L1, L2 and B components and the toxin production capacity were evaluated with an immunoassay. A total of 23 [36.5%] isolates were identified carrying simultaneously the three tested genes, from which, 20 [86.9%] showed toxigenic capacity. 26 [41.3%] isolates did not carry any of genes tested and the other 14 [22.2%] were positive for one or two of them. The results showed a high toxigenic capacity among the B. cereus isolates able to produce the HBL, indicating a potential risk for consumers.
Descritores: Bacillus cereus/isolamento & purificação
Proteínas de Bactérias/análise
Proteínas de Bactérias/genética
Laticínios/microbiologia
Proteínas Hemolisinas/análise
Proteínas Hemolisinas/genética
Leite/microbiologia
-Brasil
Bacillus cereus/genética
DNA Bacteriano/genética
Imunoensaio
Reação em Cadeia da Polimerase
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-699824
Autor: Ben Khedher, Saoussen; Jaoua, Samir; Zouari, Nabil.
Título: Application of statistical experimental design for optimisation of bioinsecticides production by sporeless Bacillus thuringiensis strain on cheap medium
Fonte: Braz. j. microbiol;44(3):927-933, July-Sept. 2013. graf, tab.
Idioma: en.
Resumo: In order to overproduce bioinsecticides production by a sporeless Bacillus thuringiensis strain, an optimal composition of a cheap medium was defined using a response surface methodology. In a first step, a Plackett-Burman design used to evaluate the effects of eight medium components on delta-endotoxin production showed that starch, soya bean and sodium chloride exhibited significant effects on bioinsecticides production. In a second step, these parameters were selected for further optimisation by central composite design. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 30 g L-1 starch, 30 g L-1 soya bean and 9g L-1 sodium chloride. When compared to the basal production medium, an improvement in delta-endotoxin production up to 50% was noted. Moreover, relative toxin yield of sporeless Bacillus thuringiensis S22 was improved markedly by using optimised cheap medium (148.5 mg delta-endotoxins per g starch) when compared to the yield obtained in the basal medium (94.46 mg delta-endotoxins per g starch). Therefore, the use of optimised culture cheap medium appeared to be a good alternative for a low cost production of sporeless Bacillus thuringiensis bioinsecticides at industrial scale which is of great importance in practical point of view.
Descritores: Bacillus thuringiensis/crescimento & desenvolvimento
Bacillus thuringiensis/metabolismo
Proteínas de Bactérias/metabolismo
Reatores Biológicos/microbiologia
Biotecnologia/métodos
Meios de Cultura/química
Endotoxinas/metabolismo
Proteínas Hemolisinas/metabolismo
-Modelos Estatísticos
Projetos de Pesquisa
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde