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Id: lil-27500
Autor: Carazas, Ignacio; Ramos, Arturo; Benites, Juan; Rodríguez, Fernando.
Título: Utilización de hematíes humanos "O" en la prueba de Waaler-Rose / Use of human hematies \"O\" in Waaler-Rose test
Fonte: Rev. serv. sanid. fuerzas polic;46(2):131-4, jul.-dic. 1985. tab.
Idioma: es.
Resumo: La obtención de una hemolisina anticarnero de alto poder es una necesidad para mejorar el sistema hemolítico; y, la posibilidad de los laboratorios pequeños para contar con un bioterio adecuado de donde obtener fácilmente los glóbulos rojos de carnero a motivado que el personal del Laboratorio Clínico del Hospital Central de la Sanidad de las Fuerzas Policiales se preocupe por la solución de este problema y llegar a implementar y mejorar la calidad de este tipo de análisis. En el presente trabajo se expone una técnica sencilla y fácil de realizar por personal capacitado, con la cual nos permite obtner altos títulos de suero hemolítico, al inyectar a un conejo, primero sangre humana total del grupo "O" por vía intracutánea, en dosis crecientes para luego continuar inyectando por vía endovenosa glóbulos rojos "O" al 20%. Obtuvimos un suero hemolítico con un título aglutinante de 1/3200. Posteriormente se realizó una técnica distinta utilizando glóbulos rojos "O" humanos sensibilizados con el suero hemolítico obtenido, obteniéndose resultados idénticos
Descritores: Fator Reumatoide/sangue
Eritrócitos/imunologia
Proteínas Hemolisinas/biossíntese
Testes de Aglutinação
-Soros Imunes
Limites: Animais
Humanos
Responsável: PE1.1 - Oficina Universitária de Biblioteca


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Id: lil-583937
Autor: Magalhães, Caroline A; Rossato, Sarita S; Barbosa, Ângela S; Santos, Thiago O dos; Elias, Waldir P; Sircili, Marcelo P; Piazza, Roxane MF.
Título: The ability of haemolysins expressed by atypical enteropathogenic Escherichia coli to bind to extracellular matrix components
Fonte: Mem. Inst. Oswaldo Cruz;106(2):146-152, Mar. 2011. ilus, graf, tab.
Idioma: en.
Projeto: Claudia Trigo Pedroso de Moraes; . FAPESP.
Resumo: Typical and atypical enteropathogenic Escherichia coli (EPEC) are considered important bacterial causes of diarrhoea. Considering the repertoire of virulence genes, atypical EPEC (aEPEC) is a heterogeneous group, harbouring genes that are found in other diarrheagenic E. coli pathotypes, such as those encoding haemolysins. Haemolysins are cytolytic toxins that lyse host cells disrupting the function of the plasma membrane. In addition, these cytolysins mediate a connection to vascular tissue and/or blood components, such as plasma and cellular fibronectin. Therefore, we investigated the haemolytic activity of 72 aEPEC isolates and determined the correlation of this phenotype with the presence of genes encoding enterohaemolysins (Ehly) and cytolysin A (ClyA). In addition, the correlation between the expression of haemolysins and the ability of these secreted proteins to adhere to extracellular matrix (ECM) components was also assessed in this study. Our findings demonstrate that a subset of aEPEC presents haemolytic activity due to the expression of Ehlys and/or ClyA and that this activity is closely related to the ability of these isolates to bind to ECM components.
Descritores: Escherichia coli Enteropatogênica/fisiologia
Proteínas de Escherichia coli/fisiologia
Matriz Extracelular
-Escherichia coli Enteropatogênica
Escherichia coli Enteropatogênica
Proteínas de Escherichia coli
Genes Bacterianos
Proteínas Hemolisinas
Fenótipo
Reação em Cadeia da Polimerase
Sorotipagem
Fatores de Virulência
Limites: Animais
Humanos
Coelhos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: biblio-989445
Autor: Luz, P M C; Specht, A; Paula-Moraes, S V; Malaquias, J V; Ferreira, L F M; Otanásio, P N; Diniz, I R.
Título: Owlet moths (Lepidoptera: Noctuoidea) associated with Bt and non- Bt soybean in the brazilian savanna / Noctuóides (Lepidoptera: Noctuoidea) associados a soja Bt e não-Bt no Cerrado brasileiro
Fonte: Braz. j. biol;79(2):248-256, Apr.-June 2019. tab, graf.
Idioma: en.
Projeto: ICMBio; . CNPq; . Empresa Brasileira de Pesquisa Agropecuária.
Resumo: Abstract The use of GMO expressing Bt toxin in soybean production has increased significantly in the last years in Brazil in order to manage the damage caused by lepidopteran pests. In this study, we compared the richness and abundance of owlet moths (Noctuoidea) associated with Bt and non-Bt soybean. We determined the temporal variations as a function of phenology, and correlated the population variations of the most common species with meteorological variables. The research was conducted at the experimental area of Embrapa Cerrados. The collection method used was differentiated being suppressive and absolute. A total of 13 species were collected, of which eight occurred on Bt soybeans. The most representative taxa were Chrysodeixis includens (72.87%), Anticarsia gemmatalis (18.17%) and Spodoptera spp (5.22%). The number of larvae belonging to species targeted by the Bt technology was 10 times lower on Bt than on non-Bt soybeans. Utetheisa ornatrix and Elaphria deltoides were recorded on soybean for the first time, observing larvae of both species in non-Bt soybean and those of U. ornatrix also in Bt soybean. Only A. gemmatalis larvae correlated (p <0.05) negatively with precipitation. This study provided field information on the abundance and species richness of owlet moths on non-Bt soybeans, associated with the effects of Bt soybean. When considering the different levels of infestation between cultivars as a criterion, larvae monitoring is of substantial importance in order to develop the lost control program.

Resumo O uso de OGM que expressam toxina Bt na produção de soja tem aumentado significativamente nos últimos anos no Brasil e são utilizados para conter os danos causados ​​pelos lepidópteros pragas. Neste estudo comparamos a riqueza e a abundância de Noctuoides (Noctuoidea) associados à soja Bt e não-Bt. Determinamos as variações temporais em função da fenologia e correlacionamos às variações populacionais das espécies mais comuns com variáveis ​​meteorológicas. A pesquisa foi conduzida na área experimental da Embrapa Cerrados. O método de coleta utilizado foi diferenciado sendo supressivo e absoluto. Um total de 13 espécies foram coletadas, das quais oito ocorreram em soja Bt. Os taxa mais representativos foram Chrysodeixis includens, Anticarsia gemmatalis e Spodoptera spp. O número de larvas pertencentes às espécies alvo da tecnologia Bt foram 10 vezes menores na soja Bt do que em soja não-Bt . Utetheisa ornatrix e Elaphria deltoides foram registradas na soja pela primeira vez, observando-se larvas de ambas espécies na soja não-Bt e as de U. ornatrix também na soja Bt. Somente as larvas de A. gemmatalis se correlacionaram (p <0,05) negativamente com a precipitação. Este estudo forneceu informações em campo sobre a abundância e riqueza de espécies na soja não- Bt, associada aos efeitos da soja Bt. A importância do monitoramento das lagartas é substancial, a fim de tomar a melhor decisão de controle, considerando-se os diferentes níveis de infestação entre cultivares como critério.
Descritores: Proteínas de Bactérias/genética
Proteínas de Bactérias/farmacologia
Endotoxinas/genética
Endotoxinas/farmacologia
Proteínas Hemolisinas/genética
Proteínas Hemolisinas/farmacologia
-Soja/genética
Soja/parasitologia
Brasil
Controle Biológico de Vetores
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/parasitologia
Larva/efeitos dos fármacos
Mariposas/efeitos dos fármacos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-788966
Autor: Azizoglu, Ugur; Ayvaz, Abdurrahman; Yılmaz, Semih; Karabörklü, Salih; Temizgul, Rıdvan.
Título: Expression of cry1Ab gene from a novel Bacillus thuringiensis strain SY49-1 active on pest insects
Fonte: Braz. j. microbiol;47(3):597-602, July-Sept. 2016. graf.
Idioma: en.
Projeto: Erciyes University; . Bilim, Sanayi ve Teknoloji Bakanlığı.
Resumo: ABSTRACT In this study, the cry1Ab gene of previously characterized and Lepidoptera-, Diptera-, and Coleoptera-active Bacillus thuringiensis SY49-1 strain was cloned, expressed and individually tested on Ephestia kuehniella (Lepidoptera: Pyralidae) and Plodia interpunctella (Lepidoptera: Pyralidae) larvae. pET-cry1Ab plasmids were constructed by ligating the cry1Ab into pET28a (+) expression vector. Constructed plasmids were transferred to an Escherichia coli BL21 (DE3) strain rendered competent with CaCl2. Isopropyl β-D-1-thiogalactopyranoside was used to induce the expression of cry1Ab in E. coli BL21(DE3), and consequently, ∼130 kDa of Cry1Ab was obtained. Bioassay results indicated that recombinant Cry1Ab at a dose of 1000 µg g-1 caused 40% and 64% mortality on P. interpunctella and E. kuehniella larvae, respectively. However, the mortality rates of Bt SY49-1 strains' spore-crystal mixture at the same dose were observed to be 70% on P. interpunctella and 90% on E. kuehniella larvae. The results indicated that cry1Ab may be considered as a good candidate in transgenic crop production and as an alternative biocontrol agent in controlling stored product moths.
Descritores: Bacillus thuringiensis/fisiologia
Proteínas de Bactérias/genética
Expressão Gênica
Controle de Insetos
Endotoxinas/genética
Proteínas Hemolisinas/genética
-Bacillus thuringiensis/ultraestrutura
Proteínas de Bactérias/metabolismo
Proteínas de Bactérias/toxicidade
Controle de Insetos/métodos
Clonagem Molecular
Endotoxinas/metabolismo
Endotoxinas/toxicidade
Proteínas Hemolisinas/metabolismo
Proteínas Hemolisinas/toxicidade
Inseticidas
Larva
Mariposas/efeitos dos fármacos
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-618190
Autor: Thangaraj, S; Bragadeeswaran, S.
Título: Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India
Fonte: J. venom. anim. toxins incl. trop. dis;18(1):53-61, 2012. ilus.
Idioma: en.
Resumo: Cnidarians comprise an old and diverse animal phylum, and possess a wide variety of biologically active substances. Sea anemones contain a diversity of interesting biologically active compounds including some potent toxins. In the present work, the sea anemones Stichodactyla mertensii and Stichodactyla gigantea, collected from the Mandapam coast, are characterized biomedically and pharmacologically. The crude protein was obtained by using methanol and aqueous extracts. The respective protein contents of S. mertensii and S. gigantea were found to be 2.10 µg/mL and 1.87 µg/mL. The methanol and aqueous extracts of S. mertensii and S. gigantea yielded six and nine bands by SDS-PAGE on 12 percent gel. In the hemolytic assay, both extracts exhibited hemolytic effect on chicken, goat, cow and human erythrocytes ('A', 'B' and 'O'). The neurotoxic effects of these crude extracts were determined in vivo using the sea shore crab Ocypode macrocera and mortality was observed. The mouse bioassay for lethality was performed on male albino mice. The crude extract of S. mertensii showed higher lethality (58 seconds at 1 mL-dose) than that of S. gigantea (2 minutes and 10 seconds at 0.75 mL-dose). The analgesic activity test was also carried out on albino mice by Eddy's hot plate and tail-flick methods. The extracts showed moderate analgesic effect by both hot-plate and tail-flick methods. These characteristics emphasize the need for the isolation and molecular characterization of new active toxins in S. mertensii and S. gigantea.(AU)
Descritores: Anêmonas-do-Mar/química
Antivenenos
Venenos de Cnidários/toxicidade
Neurotoxinas/química
-Bioensaio/métodos
Proteínas Hemolisinas/isolamento & purificação
Proteínas Hemolisinas/efeitos dos fármacos
Analgésicos/farmacologia
Limites: Animais
Masculino
Ratos
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-911518
Autor: Lopes, E. S; Cardoso, W. M; Nishi, D. M; Horn, R. V; Albuquerque, Á. H; Lima, S. V. G; Beleza, A. J. F; Gaio, F. C; Carmo, C. C; Pascoal Filho, M. N; Teixeira, R. S. C.
Título: Serogroup identification, phenotypic detection of hemolysis and extended spectrum beta-lactamases of Escherichia coli isolated from psittacine of illegal wildlife trade in Fortaleza, Brazil / Identificação de sorogrupo e detecção fenotípica de betalactamase de espectro estendido e produção de hemolisina em Escherichia coli isoladas de psitacídeos do tráfico de animais silvestres em Fortaleza-CE
Fonte: Arq. bras. med. vet. zootec. (Online);70(3):823-829, maio-jun. 2018. tab, graf.
Idioma: en.
Resumo: This study aimed to identify serogroups of Escherichia coli important for human health in isolates from psittacine of illegal wildlife trade in Ceará State. In addition, hemolysis and production of Extended Spectrum Beta-Lactamases (ESBL) was assessed in the isolates. A total of 78 E. coli strains isolated from different Psittaciformes species from a wildlife rehabilitation center in Fortaleza, Brazil. The isolates used in this study were previously identified and stored. Serogroup identification was performed using polyvalent sera for EPEC (O55, O111, O119, O114, O125, O86, O126, O127, O128), EIEC (O136, O124) and EHEC (O157). ESBL detection was performed with double disk synergy method. For hemolysis detection, isolates were inoculated in blood agar base enriched with ovine blood. Only 31 (39.7%) isolates were seropositive and the most frequent were O127, O114, O128 and O111. There was no agglutination for serogroups O55, O124, O136 or O157. Considering both seropositive and seronegative isolates, 9 (11.5%) and 35 (44.9%) presented hemolysis and ESBL production, respectively. In conclusion, the investigated psittacine from illegal wildlife trade hosted ESBL-producing E. coli strains and some belong to important serogroups often linked to severe human infections.(AU)

Este trabalho teve como objetivo identificar sorogrupos de E. coli importantes para a saúde humana, oriundos de psitacídeos provenientes do tráfico no estado do Ceará, assim como detectar atividade hemolítica e produção de betalactamase de espectro estendido (ESBL). Foram testadas 78 cepas de Escherichia coli provenientes de psitaciformes do Centro de Triagem de Animais Silvestres, Fortaleza, CE. Para a identificação dos sorogrupos, utilizaram-se soros polivalentes EPEC (O55, O111, O119, O114, O125, O86, O126, O127, O128), EIEC (O136, O124) e EHEC (O157). Para detecção de ESBL, as cepas foram submetidas ao método de aproximação de disco e, para a detecção de hemolisina, foram plaqueadas em ágar sangue base enriquecido com sangue de carneiro. No geral, 31 (39,7%) das amostras foram soropositivas. Os sorogrupos mais frequentemente detectados foram O127, O114, O128 e O111. Não houve positividade para os sorogrupos O55, O124, O136 e O157. Considerando-se as amostras sororreagentes e não sororreagentes, observou-se que nove (11,5%) e 35 (44,9%) cepas de E. coli apresentaram produção de hemolisinas e de ESBL, respectivamente. Em conclusão, constatou-se que psitacídeos provenientes do tráfico de animais silvestres albergam cepas de E. coli produtoras de ESBL e providas de importantes sorogrupos implicados em graves infecções humanas.(AU)
Descritores: beta-Lactamases
Escherichia coli/isolamento & purificação
Papagaios/microbiologia
-Proteínas Hemolisinas/análise
Sorogrupo
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-762924
Autor: Beltrame, C. O.; Côrtes, M. F.; Bandeira, P. T.; Figueiredo, A. M. S..
Título: Optimization of the RNeasy Mini Kit to obtain high-quality total RNA from sessile cells of Staphylococcus aureus
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;48(12):1071-1076, Dec. 2015. tab, graf.
Idioma: en.
Resumo: Biofilm formed by Staphylococcus aureus is considered an important virulence trait in the pathogenesis of infections associated with implantable medical devices. Gene expression analyses are important strategies for determining the mechanisms involved in production and regulation of biofilm. Obtaining intact RNA preparations is the first and most critical step for these studies. In this article, we describe an optimized protocol for obtaining total RNA from sessile cells of S. aureus using the RNeasy Mini Kit. This method essentially consists of a few steps, as follows: 1) addition of acetone-ethanol to sessile cells, 2) lysis with lysostaphin at 37°C/10 min, 3) vigorous mixing, 4) three cycles of freezing and thawing, and 5) purification of the lysate in the RNeasy column. This simple pre-kit procedure yields high-quality total RNA from planktonic and sessile cells of S. aureus.
Descritores: Técnicas Bacteriológicas/normas
Biofilmes/crescimento & desenvolvimento
RNA Bacteriano/isolamento & purificação
Staphylococcus aureus/genética
-Técnicas Bacteriológicas/métodos
Eletroforese em Gel de Ágar
Proteínas Hemolisinas/metabolismo
Staphylococcus aureus Resistente à Meticilina/genética
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Controle de Qualidade
Reação em Cadeia da Polimerase em Tempo Real
Transcrição Reversa
Staphylococcus aureus/fisiologia
Responsável: BR1.1 - BIREME


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Id: lil-759358
Autor: ELLEPOLA, Arjuna Nishantha; KHAJAH, Rana; JAYATILAKE, Sumedha; SAMARANAYAKE, Lakshman; SHARMA, Prem; KHAN, Zia.
Título: Impact of brief exposure to antifungal agents on the post-antifungal effect and hemolysin activity of oral Candida albicans
Fonte: J. appl. oral sci;23(4):412-418, July-Aug. 2015. tab.
Idioma: en.
Projeto: Kuwait University Research.
Resumo: AbstractPost-antifungal effect (PAFE) of Candida and its production of hemolysin are determinants of candidal pathogenicity. Candida albicans is the foremost aetiological agent of oral candidosis, which can be treated with polyene, azole, and echinocandin antifungals. However, once administered, the intraoral concentrations of these drugs tend to be subtherapeutic and transient due to the diluent effect of saliva and cleansing effect of the oral musculature. Hence, intra-orally, Candidamay undergo a brief exposure to antifungal drugs.Objective Therefore, the PAFE and hemolysin production of oral C. albicans isolates following brief exposure to sublethal concentrations of the foregoing antifungals were evaluated.Material and Methods A total of 50 C. albicans oral isolates obtained from smokers, diabetics, asthmatics using steroid inhalers, partial denture wearers and healthy individuals were exposed to sublethal concentrations of nystatin, amphotericin B, caspofungin, ketoconazole and fluconazole for 60 min. Thereafter, the drugs were removed and the PAFE and hemolysin production were determined by previously described turbidometric and plate assays, respectively.Results Nystatin, amphotericin B, caspofungin and ketoconazole induced mean PAFE (hours) of 2.2, 2.18, 2.2 and 0.62, respectively. Fluconazole failed to produce a PAFE. Hemolysin production of these isolates was suppressed with a percentage reduction of 12.27, 13.47, 13.33, 8.53 and 4.93 following exposure to nystatin, amphotericin B, caspofungin, ketoconazole and fluconazole, respectively.Conclusions Brief exposure to sublethal concentrations of antifungal drugs appears to exert an antifungal effect by interfering with the growth as well as hemolysin production of C. albicans.
Descritores: Antifúngicos/farmacologia
Candida albicans/efeitos dos fármacos
Candida albicans/isolamento & purificação
Farmacorresistência Fúngica/efeitos dos fármacos
Proteínas Hemolisinas/efeitos dos fármacos
-Anfotericina B/farmacologia
Estudos de Casos e Controles
Contagem de Colônia Microbiana
Candida albicans/metabolismo
Equinocandinas/farmacologia
Fluconazol/farmacologia
Proteínas Hemolisinas/metabolismo
Cetoconazol/farmacologia
Testes de Sensibilidade Microbiana
Nistatina/farmacologia
Estatísticas não Paramétricas
Fatores de Tempo
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-756568
Autor: Landim, Cinthia Silvestre; Gomes, Francisco Carlos Almeida; Zeza, Bernardete Martin; Mendrone-Júnior, Alfredo; Dinardo, Carla Luana.
Título: Prophylactic strategies for acute hemolysis secondary to plasma-incompatible platelet transfusions: correlation between qualitative hemolysin test and isohemagglutinin titration
Fonte: Rev. bras. hematol. hemoter;37(4):217-222, July-Aug. 2015. tab, ilus.
Idioma: en.
Resumo: Brazilian legislation has recently suggested the use of the qualitative hemolysin test instead of isohemagglutinin titers as prophylaxis for acute hemolysis related to plasma-incompatible platelet transfusions. The efficacy of this test in preventing hemolytic reactions has never been evaluated while isohemagglutinin titers have been extensively studied. The main objective of this study was to evaluate the correlation between the results of these two tests. The impact of each type of prophylaxis on the platelet inventory management and the ability of the qualitative hemolysin test to prevent red cell sensitization after the transfusion of incompatible units were also studied.METHODS: A total of 246 donor blood samples were evaluated using both isohemagglutinin titers and the qualitative hemolysin test, and the results were statistically compared. Subsequently, 600 platelet units were tested using the hemolysin assay and the percentage of units unsuitable for transfusion was compared to historical data using isohemagglutinin titers (cut-off: 100). Moreover, ten patients who received units with minor ABO incompatibilities that were negative for hemolysis according to the qualitative hemolysin test were evaluated regarding the development of hemolysis and red cell sensitization (anti-A or anti-B).RESULTS: Isohemagglutinin titration and the results of qualitative hemolysin test did not correlate. The routine implementation of the qualitative hemolysin test significantly increased the percentage of platelet units found unsuitable for transfusions (15-65%; p-value <0.001)...
Descritores: Sistema ABO de Grupos Sanguíneos
Incompatibilidade de Grupos Sanguíneos
Proteínas Hemolisinas
Hemólise
Transfusão de Plaquetas
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: lil-723107
Autor: Navidinia, Masoumeh; Peerayeh, Shahin Najar; Fallah, Fatemeh; Bakhshi, Bita; Sajadinia, Raheleh Sadat.
Título: Phylogenetic grouping and pathotypic comparison of urine and fecal Escherichia coli isolates from children with urinary tract infection
Fonte: Braz. j. microbiol;45(2):509-514, Apr.-June 2014. tab.
Idioma: en.
Resumo: The aim of this study was to investigate the phylogenetic background and to assess hlyD (involved in the secretion of haemolysin A) and intll (encoding a class 1 integrase) in Escherichia coli isolates derived from urinary and fecal specimens. A total of 200 E. coli isolates was collected from patients presenting with urinary tract infection (UTI) during September 2009 to September 2010 and screened for hlyD and intll genes by polymerase chain reaction (PCR). Phylogenetic analysis showed that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D) and that uropathogenic E. coli (UPEC) isolates mainly belong to groups B2 (54%) and D (34%) whereas group A (44%) and D (26%) are predominant among commensal E. coli isolates. In this study, hlyD was present in 26% of UPEC and 2% of commensal E. coli isolates. However, hemolytic activity was detected for 42% of UPEC and 6% of commensal E. coli isolates (p < 0.05). intll gene was more frequently expressed in UPEC (24%) in comparison with commensal E. coli isolates (12%). Resistance to aztreonam, co-trimoxazole and cefpodoxime were frequently found among UPEC isolates whereas commensal E. coli isolates were commonly resistant to co-trimoxazole, nalidixic acid and cefotaxime. Concluding, a considerable difference between UPEC and commensal E. coli isolates was observed regarding their phylogenetic groups, presence of class 1 integron and hlyD gene, hemolysin activity and resistance pattern. The detection of class 1 integrons and hlyD gene was higher among UPEC compared with commensal E. coli isolates. These findings may contribute for a better understanding of the factors involved in the pathogenesis of UPEC.
Descritores: Infecções por Escherichia coli/microbiologia
Escherichia coli/classificação
Fezes/microbiologia
Variação Genética
Filogenia
Infecções Urinárias/microbiologia
Urina/microbiologia
-Antibacterianos/farmacologia
Análise por Conglomerados
Farmacorresistência Bacteriana
Proteínas de Escherichia coli/genética
Escherichia coli/efeitos dos fármacos
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Genótipo
Proteínas Hemolisinas/genética
Integrases/genética
Proteínas de Membrana Transportadoras/genética
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Limites: Criança
Pré-Escolar
Feminino
Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde