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Pesquisa : D12.776.543.750.690.500 [Categoria DeCS]
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Texto completo SciELO Brasil
Metze, K
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Id: lil-365229
Autor: Vassallo, J; Godoy Júnior, C. E; Godoy, C. E; Chagas, C. A; Metze, K; Trevisan, M. A. S.
Título: Immunoexpression of CD95 in chronic gastritis and gastric mucosa-associated lymphomas
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;37(9):1397-1401, Sept. 2004. ilus, tab.
Idioma: en.
Projeto: FAEP-UNICAMP.
Resumo: CD95 (Fas/APO-1)-mediated apoptosis plays an important role in immunological regulation and is related to the pathogenesis of autoimmune diseases. Immunoexpression of CD95 has been reported to frequently occur in low grade non-Hodgkin lymphomas, especially of post-germinal center histogenesis, among which those originating in mucosa-associated lymphoid tissue (MALT lymphomas). However, there is no report comparing in situ immunoexpression of this marker in lymphomas and the hyperplastic lymphoid reaction (chronic gastritis) related to Helicobacter pylori infection. The purpose of the present research was to compare the intensity of lymphoid CD95 immunoexpression in 15 cases of H. pylori-related chronic gastritis and 15 gastric MALT lymphomas. CD95 (anti-CD95) was detected by an immunoperoxidase technique in paraffin sections using the catalyzed amplification system. Graduation of reaction intensity (percentage of CD95-positive cells) was semiquantitative, from 1+ to 4+. Nine cases of chronic gastritis were 4+, five 2+ and one 1+. Three lymphomas were 4+, three 3+, four 2+, four 1+, and one was negative. Although 14 of 15 lymphomas were positive for CD95, the intensity of the reaction was significantly weaker compared to that obtained with gastric tissue for patients with gastritis (P = 0.03). The difference in CD95 immunoexpression does not seem to be useful as an isolated criterion in the differential diagnosis between chronic gastritis and MALT lymphomas since there was overlapping of immunostaining patterns. However, it suggests the possibility of a pathogenetic role of this apoptosis-regulating protein in MALT lymphomas.
Descritores: Receptor fas
Apoptose
Gastrite
Linfoma de Zona Marginal Tipo Células B
Neoplasias Gástricas
-Biomarcadores Tumorais
Doença Crônica
Diagnóstico Diferencial
Infecções por Helicobacter
Helicobacter pylori
Técnicas Imunoenzimáticas
Imuno-Histoquímica
Limites: Seres Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: lil-304669
Autor: Boechat, L. H. B; Vilella, C. A; Zollner, R. L.
Título: Effect of iodide on Fas, Fas-ligand and Bcl-w mRNA expression in thyroid of NOD mice pretreated with methimazole
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;35(3):289-295, Mar. 2002. ilus, tab.
Idioma: en.
Projeto: CNPq.
Resumo: Nonobese diabetic (NOD) mice and a derived strain, NOD.H.2h4, have been used as a model for experimental spontaneous thyroiditis and thyroiditis induced by iodide excess after a goiter-inducing period. Some authors have proposed that iodide, given after methimazole or propylthiouracil, is capable of inducing apoptosis in thyroid cells and that anti-thyroid drugs can modulate the expression of apoptosis components such as Fas and its ligand (Fas-L). Here we evaluated the effect of potassium iodide (20 æg/animal for 4 days, ip) given to NOD mice at the 10th week of life after exposure to methimazole (1 mg/ml) in drinking water from the 4th to the 10th week of life. Fas, Fas-L and Bcl-w expression were analyzed semiquantitatively by RT-PCR immediately after potassium iodide administration (group MI44D) or at week 32 (MI32S). Control groups were added at 10 (C10) and 32 weeks (C32), as well as a group that received only methimazole (CM10). An increase in the expression of Fas-L and Bcl-w (P<0.01, ANOVA) was observed in animals of group MI44D, while Fas was expressed at higher levels (P = 0.02) in group C32 (72.89 ± 47.09 arbitrary units) when compared to group C10 (10.8 ± 8.55 arbitrary units). Thus, the analysis of Fas-L and Bcl-w expression in the MI44D group and Fas in group C32 allowed us to detect two different patterns of expression of these apoptosis components in thyroid tissue of NOD mice
Descritores: Receptor fas
Apoptose
Iodeto de Potássio
RNA Mensageiro
Glândula Tireoide
Tireoidite
-Antitireóideos
Apoptose
Modelos Animais de Doenças
Eletroforese
Expressão Gênica
Glicoproteínas de Membrana
Metimazol
Camundongos Endogâmicos NOD
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME



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