Base de dados : LILACS
Pesquisa : D12.776.543.750.690.500 [Categoria DeCS]
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Texto completo SciELO Chile
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Id: biblio-950727
Autor: Urriola-Muñoz, Paulina; Lizama, Carlos; Lagos-Cabré, Raúl; Reyes, Juan G; Moreno, Ricardo D.
Título: Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
Fonte: Biol. Res;47:1-12, 2014. ilus, graf.
Idioma: en.
Projeto: FONDECYT.
Resumo: BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. RESULTS: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of spermatogenesis. Interestingly their protein levels and cell surface localization in adult rats were stage-specific, suggesting activation of these enzymes at particular events of rat spermatogenesis. CONCLUSIONS: Therefore, these results show that ADAM10 and ADAM17 protein levels and subcellular (cell surface) localization are regulated during rat spermatogenesis.
Descritores: Espermatogênese/fisiologia
Espermatozoides/metabolismo
Proteínas ADAM/metabolismo
-Túbulos Seminíferos/química
Células de Sertoli/citologia
Células de Sertoli/metabolismo
Espermátides/citologia
Espermátides/metabolismo
Testículo/anatomia & histologia
RNA Mensageiro/análise
Imuno-Histoquímica
Diferenciação Celular/fisiologia
Ratos Sprague-Dawley
Apoptose/fisiologia
Receptor fas/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proteínas ADAM/análise
Proteína ADAM10
Proteína ADAM17
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1041727
Autor: Sheremet, M. I; Sydorchuk, L. P; Shidlovskyi, V. O; Bedenyuk, A. D; Sydorchuk, R. I; Shidlovskyi, O. V; Batig, V. M; Maksymyuk, V. V; Bezruk, V. V; Bezruk, T. O; Chympoi, K. A; Gresko, M. D.
Título: Efecto combinado de la interacción genética sobre el desarrollo del bocio nodular con tiroiditis autoinmune y adenoma tiroideo en los habitantes de Bucovina del Norte / Combined effect of gene-gene interaction on the development of nodular goiter with autoimmune thyroiditis and thyroid adenoma in the inhabitants of Northern Bukovyna
Fonte: Rev. argent. endocrinol. metab;55(1):50-59, mar. 2018. graf.
Idioma: en.
Resumo: ABSTRAC This article presents the results of a comprehensive analysis of the combined influence of genetic polymorphisms associated with various links of apoptosis regulation (BCL-2, CTLA-4 and APO-1/Fas) on the development of nodular goiter with autoimmune thyroiditis and thyroid adenoma in the studied population. The analysis was performed using the Multifactor Dimensionality Reduction (MDR) method by calculating the prediction potential. Graphic models of gene-gene interaction with the highest cross-validation consistency created by the MDR method showed complex "synergistic or independent" impact of polymorphic loci of the CTLA-4 (+49G/A), Fas (-1377G/A) and BCL-2 (63291411 A>G) genes on the onset of thyroid pathology in general, or its individual types (nodular goiter with autoimmune thyroiditis and thyroid adenoma) in the population of Northern Bukovyna.

RESUMEN Este artículo presenta los resultados de un análisis exhaustivo de la influencia combinada de polimorfismos genéticos asociados a diversos enlaces en la regulación de la apoptosis (BCL-2, CTLA-4 y APO-1/FAS) sobre el desarrollo de bocio nodular con tiroiditis autoinmune y adenoma tiroideo en la población estudiada. Para ello, se utilizó el método de reducción de dimensionalidad multifactorial (MDR) mediante el cálculo de los potenciales de predicción. Los modelos gráficos de interacción gen-gen con la mayor consistencia de validación cruzada creada por el método MDR mostraron un complejo impacto «sinérgico o independiente¼ de los loci polimórficos de los genes CTLA-4 (+49G/A), FAS (-1377G/A) y BCL-2 (63291411A>G) en el inicio de la patología tiroidea en general, o sus tipos individuales (bocio nodular con tiroiditis autoinmune y adenoma tiroideo) en la población de Bucovina septentrional.
Descritores: Polimorfismo Genético/fisiologia
Tireoidite Autoimune/genética
Neoplasias da Glândula Tireoide/genética
Bócio Nodular/fisiopatologia
Bócio Nodular/genética
-Apoptose/fisiologia
Receptor fas/análise
Genes bcl-2/genética
Redução Dimensional com Múltiplos Fatores/métodos
Abatacepte/análise
Bócio Nodular/etiologia
Limites: Humanos
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Tipo de Publ: Estudo Multicêntrico
Ensaio Clínico Controlado
Estudo de Avaliação
Estudo Clínico
Responsável: AR635.1 - FCVyS - Servicio de Información y Documentación


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Texto completo SciELO Brasil
Metze, K
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Id: lil-365229
Autor: Vassallo, J; Godoy Júnior, C. E; Godoy, C. E; Chagas, C. A; Metze, K; Trevisan, M. A. S.
Título: Immunoexpression of CD95 in chronic gastritis and gastric mucosa-associated lymphomas
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;37(9):1397-1401, Sept. 2004. ilus, tab.
Idioma: en.
Projeto: FAEP-UNICAMP.
Resumo: CD95 (Fas/APO-1)-mediated apoptosis plays an important role in immunological regulation and is related to the pathogenesis of autoimmune diseases. Immunoexpression of CD95 has been reported to frequently occur in low grade non-Hodgkin lymphomas, especially of post-germinal center histogenesis, among which those originating in mucosa-associated lymphoid tissue (MALT lymphomas). However, there is no report comparing in situ immunoexpression of this marker in lymphomas and the hyperplastic lymphoid reaction (chronic gastritis) related to Helicobacter pylori infection. The purpose of the present research was to compare the intensity of lymphoid CD95 immunoexpression in 15 cases of H. pylori-related chronic gastritis and 15 gastric MALT lymphomas. CD95 (anti-CD95) was detected by an immunoperoxidase technique in paraffin sections using the catalyzed amplification system. Graduation of reaction intensity (percentage of CD95-positive cells) was semiquantitative, from 1+ to 4+. Nine cases of chronic gastritis were 4+, five 2+ and one 1+. Three lymphomas were 4+, three 3+, four 2+, four 1+, and one was negative. Although 14 of 15 lymphomas were positive for CD95, the intensity of the reaction was significantly weaker compared to that obtained with gastric tissue for patients with gastritis (P = 0.03). The difference in CD95 immunoexpression does not seem to be useful as an isolated criterion in the differential diagnosis between chronic gastritis and MALT lymphomas since there was overlapping of immunostaining patterns. However, it suggests the possibility of a pathogenetic role of this apoptosis-regulating protein in MALT lymphomas.
Descritores: Receptor fas
Apoptose
Gastrite
Linfoma de Zona Marginal Tipo Células B
Neoplasias Gástricas
-Biomarcadores Tumorais
Doença Crônica
Diagnóstico Diferencial
Infecções por Helicobacter
Helicobacter pylori
Técnicas Imunoenzimáticas
Imuno-Histoquímica
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: lil-304669
Autor: Boechat, L. H. B; Vilella, C. A; Zollner, R. L.
Título: Effect of iodide on Fas, Fas-ligand and Bcl-w mRNA expression in thyroid of NOD mice pretreated with methimazole
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;35(3):289-295, Mar. 2002. ilus, tab.
Idioma: en.
Projeto: CNPq.
Resumo: Nonobese diabetic (NOD) mice and a derived strain, NOD.H.2h4, have been used as a model for experimental spontaneous thyroiditis and thyroiditis induced by iodide excess after a goiter-inducing period. Some authors have proposed that iodide, given after methimazole or propylthiouracil, is capable of inducing apoptosis in thyroid cells and that anti-thyroid drugs can modulate the expression of apoptosis components such as Fas and its ligand (Fas-L). Here we evaluated the effect of potassium iodide (20 æg/animal for 4 days, ip) given to NOD mice at the 10th week of life after exposure to methimazole (1 mg/ml) in drinking water from the 4th to the 10th week of life. Fas, Fas-L and Bcl-w expression were analyzed semiquantitatively by RT-PCR immediately after potassium iodide administration (group MI44D) or at week 32 (MI32S). Control groups were added at 10 (C10) and 32 weeks (C32), as well as a group that received only methimazole (CM10). An increase in the expression of Fas-L and Bcl-w (P<0.01, ANOVA) was observed in animals of group MI44D, while Fas was expressed at higher levels (P = 0.02) in group C32 (72.89 ± 47.09 arbitrary units) when compared to group C10 (10.8 ± 8.55 arbitrary units). Thus, the analysis of Fas-L and Bcl-w expression in the MI44D group and Fas in group C32 allowed us to detect two different patterns of expression of these apoptosis components in thyroid tissue of NOD mice
Descritores: Receptor fas
Apoptose
Iodeto de Potássio
RNA Mensageiro
Glândula Tireoide
Tireoidite
-Antitireóideos
Apoptose
Modelos Animais de Doenças
Eletroforese
Expressão Gênica
Glicoproteínas de Membrana
Metimazol
Camundongos Endogâmicos NOD
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME



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