||Zeng, Qiangcheng; Guo, Yong; Liu, Yongming; Li, Ruixin; Zhang, Xinchang; Liu, Lu; Wang, Yang; Zhang, Xizheng; Zou, Xianqiong.|
||Integrin-ß1, not integrin-ß5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strain|
||Biol. Res;48:1-8, 2015. graf, tab.
||National Nature Science Foundation of China; . Science Foundation of Tianjin; . Shandong Provincial Key Laboratory of Functional Macromolecular Biophysics.
||BACKGROUND: Mechanical strain plays a great role in growth and differentiation of osteoblast. A previous study indicated that integrin-ß (ß1, ß5) mediated osteoblast proliferation promoted by mechanical tensile strain. However, the involvement of integrin-ß; in osteoblastic differentiation and extracellular matrix (ECM) formation induced by mechanical tensile strain, remains unclear. RESULTS: After transfection with integrin-ß1 siRNA or integrin-ß5 siRNA, mouse MC3T3-E1 preosteoblasts were cultured in cell culture dishes and stimulated with mechanical tensile strain of 2500 microstrain (µÎµ) at 0.5 Hz applied once a day for 1 h over 3 or 5 consecutive days. The cyclic tensile strain promoted osteoblastic differentiation of MC3T3-E1 cells. Transfection with integrin-ß1 siRNA attenuated the osteoblastic diffenentiation induced by the tensile strain. By contrast, transfection with integrin-ß5 siRNA had little effect on the osteoblastic differentiation induced by thestrain. At thesametime, theresultofECM formation promoted by the strain, was similar to the osteoblastic differentiation. CONCLUSION: Integrin-ß1 mediates osteoblast differentiation and osteoblastic ECM formation promoted by cyclic tensile strain, and integrin-ß5 is not involved in the osteoblasts response to the tensile strain.|
Resistência à Tração/fisiologia
Cadeias beta de Integrinas/fisiologia
RNA Interferente Pequeno
Proliferação de Células/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
| Tipo de Publ:
||Research Support, Non-U.S. Gov't|
||CL1.1 - Biblioteca Central|