Base de dados : LILACS
Pesquisa : D12.776.543.750.705.852.760.238 [Categoria DeCS]
Referências encontradas : 4 [refinar]
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Id: lil-716275
Autor: Mesquita Júnior, D.; Cruvinel, W.M.; Araujo, J.A.P.; Salmazi, K.C.; Kallas, E.G.; Andrade, L.E.C..
Título: Imbalanced expression of functional surface molecules in regulatory and effector T cells in systemic lupus erythematosus
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;47(8):662-669, 08/2014. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Regulatory T (TREG) cells play an important role in maintaining immune tolerance and avoiding autoimmunity. We analyzed the expression of membrane molecules in TREG and effector T cells in systemic lupus erythematosus (SLE). TREG and effector T cells were analyzed for the expression of CTLA-4, PD1, CD28, CD95, GITR, HLA-DR, OX40, CD40L, and CD45RO in 26 patients with active disease, 31 with inactive disease, and 26 healthy controls. TREG cells were defined as CD25+/highCD127Ø/lowFoxP3+, and effector T cells were defined as CD25+CD127+FoxP3Ø. The ratio of TREG to effector T cells expressing GITR, PD1, HLA-DR, OX40, CD40L, and CD45RO was determined in the three groups. The frequency of TREG cells was similar in patients with SLE and controls. However, SLE patients had a decreased frequency of CTLA-4+TREG and CD28+TREG cells and an increased frequency of CD40L+TREG cells. There was a decrease in the TREG/effector-T ratio for GITR+, HLA-DR+, OX40+, and CD45RO+ cells, and an increased ratio of TREG/effector-T CD40L+ cells in patients with SLE. In addition, CD40L+TREG cell frequency correlated with the SLE disease activity index (P=0.0163). In conclusion, our findings showed several abnormalities in the expression of functionally critical surface molecules in TREG and effector T cells in SLE that may be relevant to the pathogenesis of this disease.
Descritores: Antígenos de Superfície/metabolismo
Leucócitos Mononucleares/metabolismo
Lúpus Eritematoso Sistêmico/imunologia
Linfócitos T Reguladores/imunologia
-Análise de Variância
/análise
ANTIGENS, CDABORTION, HABITUAL/análise
/análise
ANTIGENS, CDABSORPTION/análise
/análise
ANTIGENS, CDACETIC ANHYDRIDES/análise
/análise
CDABRUPTIO PLACENTAE LIGAND/análise
/análise
CTLA-ABBREVIATIONS AS TOPIC ANTIGEN/análise
Citometria de Fluxo
Fatores de Transcrição Forkhead/análise
Proteína Relacionada a TNFR Induzida por Glucocorticoide/análise
Antígenos HLA-DR/análise
/análise
INTERLEUKIN-ABDOMINAL INJURIES RECEPTOR ALPHA SUBUNIT/análise
/análise
INTERLEUKIN-TEMEFOS RECEPTOR ALPHA SUBUNIT/análise
Lúpus Eritematoso Sistêmico/metabolismo
Lúpus Eritematoso Sistêmico/fisiopatologia
Receptor de Morte Celular Programada 1/análise
/análise
RECEPTORS, OXABRUPTIO PLACENTAE/análise
Estatísticas não Paramétricas
Limites: Adulto
Feminino
Seres Humanos
Masculino
Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-679528
Autor: Padovani, Cacilda Tezelli Junqueira; Bonin, Camila Mareti; Tozetti, Ines Aparecida; Ferreira, Alda Maria Teixeira; Fernandes, Carlos Eurico dos Santos; Costa, Izaias Pereira da.
Título: Glucocorticoid-induced tumor necrosis factor receptor expression in patients with cervical human papillomavirus infection
Fonte: Rev. Soc. Bras. Med. Trop;46(3):288-292, May-Jun/2013. tab, graf.
Idioma: en.
Resumo: Introduction The progression of human papillomavirus (HPV) infection in the anogenital tract has been associated with the involvement of cells with regulatory properties. Evidence has shown that glucocorticoid-induced tumor necrosis factor receptor (GITR) is an important surface molecule for the characterization of these cells and proposes that GITR ligand may constitute a rational treatment for many cancer types. We aimed to detect the presence of GITR and CD25 in cervical stroma cells with and without pathological changes or HPV infection to better understand the immune response in the infected tissue microenvironment. Methods We subjected 49 paraffin-embedded cervical tissue samples to HPV DNA detection and histopathological analysis, and subsequently immunohistochemistry to detect GITR and CD25 in lymphocytes. Results We observed that 76.9% of all samples with high GITR expression were HPV-positive regardless of histopathological findings. High GITR expression (77.8%) was predominant in samples with ≥1,000 RLU/PCB. Of the HPV-positive samples negative for intraepithelial lesion and malignancy, 62.5% had high GITR expression. High GITR expression was observed in both carcinoma and high-grade squamous intraepithelial lesion (HSIL) samples (p = 0.16). CD25 was present in great quantities in all samples. Conclusions The predominance of high GITR expression in samples with high viral load that were classified as HSIL and carcinoma suggests that GITR+ cells can exhibit regulatory properties and may contribute to the progression of HPV-induced cervical neoplasia, emphasizing the importance of GITR as a potential target for immune therapy of cervical cancer and as a disease evolution biomarker. .
Descritores: Neoplasia Intraepitelial Cervical/imunologia
Proteína Relacionada a TNFR Induzida por Glucocorticoide/análise
/análise
INTERLEUKIN-TEMEFOS RECEPTOR ALPHA SUBUNIT/análise
Infecções por Papillomavirus/imunologia
Displasia do Colo do Útero/virologia
Neoplasias do Colo do Útero/virologia
-Neoplasia Intraepitelial Cervical/virologia
Progressão da Doença
Imuno-Histoquímica
Infecções por Papillomavirus/complicações
Linfócitos T Reguladores/imunologia
Biomarcadores Tumorais/análise
Displasia do Colo do Útero/imunologia
Neoplasias do Colo do Útero/imunologia
Limites: Adulto
Feminino
Seres Humanos
Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-586507
Autor: Velavan, T. P; Bechlars, S; Huang, X; Kremsner, P. G; Kun, J. F. J.
Título: Novel regulatory SNPs in the promoter region of the TNFRSF18 gene in a Gabonese population
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;44(5):418-420, May 2011. ilus, tab.
Idioma: en.
Resumo: Parasites are accountable for driving diversity within immune gene families. We identified and investigated regulatory single nucleotide polymorphisms (SNPs) in the promoter regions of the tumor necrosis factor receptor superfamily member 18 (TNFRSF18) gene by direct sequencing in a group of male Gabonese individuals exposed to a wide array of parasitic diseases such as malaria, filariasis and schistosomiasis. Two new promoter variants were identified in 40 individuals. Both novel variants were heterozygous and were linked to SNP #rs3753344 (C/T), which has been described. One of the SNP variants (ss2080581728) was close to the general transcription factor site, the TATA box. We further validated these new promoter variants for their allelic gene expression using transient transfection assays. One new promoter variant with two base changes (C/T - ss2080581728/rs3753344) displayed an altered expression of the marker gene. Both novel variants remained less active at the non-induced state in comparison to the major allele. The allele frequencies observed in this study were consistent with data for other African populations. The detection and analysis of these human immune gene polymorphisms contribute to a better understanding of the interaction between host-parasite and expression of Treg activity.
Descritores: Proteína Relacionada a TNFR Induzida por Glucocorticoide/genética
Interações Hospedeiro-Parasita/genética
Doenças Parasitárias/genética
Polimorfismo de Nucleotídeo Único/genética
Regiões Promotoras Genéticas/genética
-Gabão
Frequência do Gene
Interações Hospedeiro-Parasita/imunologia
Reação em Cadeia da Polimerase
Doenças Parasitárias/imunologia
Transfecção
Limites: Seres Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-564141
Autor: Ramirez, E; Cartier, L; Rodriguez, L; Alberti, C; Valenzuela, M. A.
Título: In vivo fluctuation of Tax, Foxp3, CTLA-4, and GITR mRNA expression in CD4+CD25+ T cells of patients with human T-lymphotropic virus type 1-associated myelopathy
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;43(11):1109-1115, Nov. 2010. ilus, tab.
Idioma: en.
Resumo: HTLV-1 Tax expression exerts an inhibitory effect on the Foxp3 transcription factor in CD4+CD25+ T-regulatory cells (Treg). For a better understanding of the role of Tax mRNA in the gene expression of cellular markers we measured Tax, Foxp3, CTLA-4, GITR, TGF-β, and IL-10 mRNA in Treg cells of 50 patients with human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP; 27 women and 23 men; mean age: 56.7 years). The control group consisted of 23 non-infected subjects (12 women and 11 men) with a mean age of 51.3 years. Real-time PCR was used to measure mRNA of Tax proteins and several cellular markers of Treg function. Determinations revealed a high level of Tax mRNA in HAM/TSP (124.35 copies/100 CD4+CD25+ T cells). Foxp3, GITR, and CTLA-4 mRNA levels were lower in HAM/TSP patients (mean ± SD, 22.07 ± 0.78, 9.63 ± 0.36, and 4.54 ± 0.39, respectively) than in non-infected controls (47.15 ± 12.94, 22.14 ± 1.91, and 21.07 ± 2.31). Both groups had similar levels of TGF-β and IL-10. An inverse relationship was found between Tax levels and Foxp3, CTLA-4, and GITR levels. Conversely, there was a direct correlation between levels of Foxp3, GITR, and CTLA-4. Disease severity and evolution time did not correlate with Tax or Foxp3 levels. The present results suggest that Tax and Foxp3 mRNA vary with the same degree of disease severity in HAM/TSP patients. Tax fluctuations may affect CTLA-4 and GITR expression via the Foxp3 pathway, causing virus-induced dysfunction of CD4+CD25+ T cells in HAM/TSP patients.
Descritores: /metabolismo
CTLA-ABBREVIATIONS AS TOPIC ANTIGEN/metabolismo
Fatores de Transcrição Forkhead/metabolismo
Produtos do Gene tax/metabolismo
Proteína Relacionada a TNFR Induzida por Glucocorticoide/metabolismo
Vírus 1 Linfotrópico T Humano
Paraparesia Espástica Tropical/sangue
-Biomarcadores/sangue
Biomarcadores/metabolismo
Estudos de Casos e Controles
CDABBREVIATIONS AS TOPIC-POSITIVE T-LYMPHOCYTES
/sangue
CTLA-ABBREVIATIONS AS TOPIC ANTIGEN/sangue
Fatores de Transcrição Forkhead/sangue
Produtos do Gene tax/sangue
Proteína Relacionada a TNFR Induzida por Glucocorticoide/sangue
Paraparesia Espástica Tropical/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
RNA Mensageiro/sangue
Índice de Gravidade de Doença
Fator de Crescimento Transformador beta/sangue
Fator de Crescimento Transformador beta/metabolismo
Limites: Adulto
Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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