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Id: biblio-1089266
Autor: Silva, Raphael Carvalho e; Reis, Marilia Bianchini Lemos; Arid, Juliana; Flores, Elvira Katherine Barriga; Cruz, Giuseppe Valduga; Marañón-Vásquez, Guido A; Souza, Lizete Karla Filgueiras de; Novaes Jr, Arthur Belém; Queiroz, Alexandra Mussolino de; Küchler, Erika Calvano.
Título: Association between genetic polymorphisms in rank, rankl and opg and peri-implant diseases in patients from the Amazon Region
Fonte: Braz. dent. j;31(1):63-68, Jan.-Feb. 2020. tab, graf.
Idioma: en.
Projeto: FAPESP; . ECK; . FAPESP; . CAPES.
Resumo: Abstract The present study evaluated polymorphisms in RANK, RANKL and OPG-encoding genes to assess whether they are associated with mucositis and peri-implantitis in a population from the Brazilian Amazon region. One hundred and fourteen patients with dental implants were included in the study. After clinical and radiographic examination, the sample was categorized into 4 groups, according to the peri-implant status: Healthy (n=71), Mucositis (n=30), Peri-implantitis (n=13) and Diseased (Mucositis + Peri-implantitis, n=43). Genomic DNA was extracted from buccal cells from saliva, and the genetic polymorphism in osteoprotegerin (OPG), Kappa nuclear factor activator receptor (RANKL) and nuclear kappa factor activator receptor (RANK) were genotyped by the real time PCR. Univariate and multivariate statistical analyses were performed to compare clinical variables among groups and to evaluate genotypes and alleles distributions and the established alpha was 5%. Age, peri-implant biotype, diabetes and presence of peri-implant biofilm were associated with mucositis (p<0.05) and peri-implantitis (p<0.05). Smoking, alcoholism, and periodontal biofilms were also associated with the presence of peri-implantitis (p<0.05). Univariate and multivariate analysis did not demonstrate an association of peri-implantitis or mucositis with any genetic polymorphism in RANK (rs3826620), RANKL (rs9594738) and OPG (rs2073618) (p>0.05). The studied genetic polymorphism in RANK, RANKL and OPG were not associated with mucositis and peri-implantitis in a Brazilian population from the Amazon region.

Resumo O presente estudo avaliou a associação da predisposição clínica e dos fatores genéticos com a presença de doenças peri-implantares. Cento e quatorze pacientes com implantes dentais instalados na Clínica de Especialização do Amazonas, Brazil, foram incluidos no estudo. Após exame clínico e radiográfico, a amostra foi categorizada em 4 grupos, de acordo com o Status peri-implantar: saúde (n=71), mucosite (n=30), peri-implantite (n=13) e doentes (mucosite + peri-implantite). DNA genômico foi extraído de células orais da saliva, e o polimorfismo genético em osteoprotegerina (OPG), ligante do receptor ativador do fator Kappa nuclear (RANKL) e receptor ativador do fator Kappa nuclear (RANK) foram genotipados por PCR em tempo real. O estudo se propôs a avaliar se os polimorfismos em RANK, RANKL e OPG estão envolvidos na patogênese da mucosite e da peri-implantite, e avaliar também a presença de fatores de risco moduladores da resposta em uma população brasileira. Idade, biotipo peri-implantar, diabetes e presença de biofilme peri-implantar foram associados a mucosite (p<0.05) e peri-implantite (p<0.05). Tabagismo, alcoolismo e biofilme periodontal também foram associados com a presença de peri-implantite (p<0.05). Análise univariada e multivariada não demonstraram associação de peri-implantite ou mucosite com os polimorfismos genéticos em RANK (rs3826620), RANKL (rs9594738) e OPG (rs2073618) (p>0.05). Os polimorfismos genéticos estudados não foram associados com mucosite e peri-implantite em uma população brasileira da região Amazônica.
Descritores: Implantes Dentários
Ligante RANK/genética
Receptor Ativador de Fator Nuclear kappa-B/genética
Osteoprotegerina/genética
Peri-Implantite
-Polimorfismo Genético
Brasil
Mucosa Bucal
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-954511
Autor: Barreiros, Driely; Pucinelli, Carolina Maschietto; Oliveira, Katharina Morant Holanda de; Paula-Silva, Francisco Wanderley Garcia; Nelson Filho, Paulo; Silva, Lea Assed Bezerra da; Küchler, Erika Calvano; Silva, Raquel Assed Bezerra da.
Título: Immunohistochemical and mRNA expression of RANK, RANKL, OPG, TLR2 and MyD88 during apical periodontitis progression in mice
Fonte: J. appl. oral sci;26:e20170512, 2018. tab, graf.
Idioma: en.
Resumo: Abstract Objective To evaluate and correlate, in the same research, the mRNA expression and the staining of RANK, RANKL, OPG, TLR2 and MyD88 by immunohistochemistry in the apical periodontitis (AP) progression in mice. Material and Methods AP was induced in the lower first molars of thirty-five C57BL/6 mice. They were assigned to four groups according to their euthanasia periods (G0, G7, G21 and G42). The jaws were removed and subjected to histotechnical processing, immunohistochemistry and real-time reverse transcription-PCR (qRT-PCR). Data were analyzed with parametric and nonparametric tests (α=0.05). Results An increase of positive immunoreactivity for RANK, RANKL, OPG, TLR2 and MyD88 was observed over time (p<0.05). The RANKL expression was different between the groups G0 and G42, G21 and G42 (p=0.006), with G42 presenting the higher expression in both comparations. The OPG expression was statistically different between the groups G0 and G7, G7 and G21 and G7 and G42 (p<0.001), with G7 presenting higher expression in all the time points. The TLR2 expression was different between the groups G0 and G42 (p=0.03), with G42 showing the higher expression. The MyD88 expression presented a statistical significant difference between groups G7, G21 and G42 compared with G0 (p=0.01), with G0 presenting the smallest expression in all the comparisons. The Tnfrsf11/Tnfrsf11b (RANKL/OPG) ratio increased with the AP progression (p=0.002). A moderate positive correlation between MyD88 and RANKL (r=0.42; p=0.03) and between MyD88 and TLR2 (r=0.48; p<0.0001) was observed. Conclusion The expression of the RANK, RANKL, OPG, MyD88 and TLR2 proteins as well as the ratio Tnfrsf11/Tnfrsf11b (RANKL/OPG) increased with AP progression. There was also a moderate positive correlation between the expression Myd88-Tnfrsf11 and Tlr2-Myd88, suggesting the relevance of Tlr2-Myd88 in bone loss due to bacterial infection.
Descritores:
Periodontite Periapical/metabolismo
RNA Mensageiro/análise
Ligante RANK/análise
Fator 88 de Diferenciação Mieloide/análise
Osteoprotegerina/análise
-Periodontite Periapical/patologia
Valores de Referência
Imuno-Histoquímica
Expressão Gênica
Progressão da Doença
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Receptor 2 Toll-Like/análise
Camundongos Endogâmicos C57BL
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-954523
Autor: Hassumi, Jaqueline Suemi; Mulinari-Santos, Gabriel; Fabris, André Luis da Silva; Jacob, Ricardo Garcia Mureb; Gonçalves, Alaíde; Rossi, Ana Cláudia; Freire, Alexandre Rodrigues; Faverani, Leonardo Pérez; Okamoto, Roberta.
Título: Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis
Fonte: J. appl. oral sci;26:e20170326, 2018. graf.
Idioma: en.
Projeto: UNESP; . São Paulo Research Foundation.
Resumo: Abstract Alveolar bone healing after upper incisor extraction in rats is a classical model of preclinical studies. The underlying morphometric, cellular and molecular mechanism, however, remains imprecise in a unique study. Objectives The aim of this study was therefore to characterize the alveolar bone healing after upper incisor extraction in rats by micro computed tomographic (Micro-CT), immunohistochemical and real-time polymerase chain reaction (RT-PCR) analysis. Material and Methods Thirty animals (Rattus norvegicus, Albinus Wistar) were divided into three groups after upper incisors extraction at 7, 14, and 28 days. Micro-CT was evaluated based on the morphometric parameters. Subsequently, the histological analyses and immunostaining of osteoprotegerin (OPG), receptor activator of nuclear kappa B ligand (RANKL) and tartrate resistant acid phosphate (TRAP) was performed. In addition, RT-PCR analyses of OPG, RANKL, the runt-related transcription factor 2 (RUNX2), osteocalcin (OC), osteopontin (OPN), osterix (OST) and receptor activator of nuclear kappa B (RANK) were performed to determine the expression of these proteins in the alveolar bone healing. Results Micro-CT: The morphometric parameters of bone volume and trabecular thickness progressively increased over time. Consequently, a gradual decrease in trabecular separation, trabecular space and total bone porosity was observed. Immunohistochemical: There were no differences statistically significant between the positive labeling for OPG, RANKL and TRAP in the different periods. RT-PCR: At 28 days, there was a significant increase in OPG expression, while RANKL expression and the RANKL/OPG ratio both decreased over time. Conclusion Micro-CT showed the newly formed bone had favorable morphometric characteristics of quality and quantity. Beyond the RUNX2, OC, OPN, OST, and RANK proteins expressed in the alveolar bone healing, OPG and RANKL activity showed to be essential for activation of basic multicellular units during the alveolar bone healing.
Descritores: Cicatrização/fisiologia
Remodelação Óssea/fisiologia
Alvéolo Dental/fisiologia
Alvéolo Dental/diagnóstico por imagem
-Valores de Referência
Fatores de Tempo
Extração Dentária
Fatores de Transcrição/análise
Imuno-Histoquímica
Expressão Gênica
Osteocalcina/análise
Reprodutibilidade dos Testes
Ratos Wistar
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Subunidade alfa 1 de Fator de Ligação ao Core/análise
Osteopontina/análise
Ligante RANK/análise
Osteoprotegerina/análise
Microtomografia por Raio-X
Fosfatase Ácida Resistente a Tartarato/análise
Limites: Humanos
Masculino
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-951663
Autor: Zhang, L; Ding, Y; Rao, G Z; Miao, D.
Título: Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;49(4):e4324, 2016. tab, graf.
Idioma: en.
Resumo: The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.
Descritores: Ligamento Periodontal/efeitos dos fármacos
Interleucina-10/farmacologia
Ligante RANK/metabolismo
Osteoprotegerina/metabolismo
Fibroblastos/efeitos dos fármacos
Glucose/farmacologia
-Ligamento Periodontal/citologia
Fatores de Tempo
RNA Mensageiro/análise
Regulação para Baixo
Regulação para Cima
Células Cultivadas
Western Blotting
Análise de Variância
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fibroblastos/metabolismo
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-984569
Autor: Gomes, Alex Martins; Douglas-de-Oliveira, Dhelfeson Willya; Ferreira, Sérgio Diniz; Silva, Tarcília Aparecida da; Cota, Luís Otávio Miranda; Costa, Fernando Oliveira.
Título: Periodontal disease, peri-implant disease and levels of salivary biomarkers IL-1ß, IL-10, RANK, OPG, MMP-2, TGF-ß and TNF-α: follow-up over 5 years
Fonte: J. appl. oral sci;27:e20180316, 2019. tab.
Idioma: en.
Resumo: Abstract Objective The aim of this study was to evaluate the levels of salivary biomarkers IL-1β, IL-10, RANK, OPG, MMP-2, TG-β and TNF-α in individuals with diagnosis of peri-implant mucositis in the absence or presence of periodontal and peri-implant maintenance therapy (TMPP) over 5 years. Material and Methods Eighty individuals diagnosed with peri-implant mucositis were divided into two groups: one group that underwent periodontal and peri-implant regularly maintenance therapy, called GTP (n=39), and a second group that received no regular maintenance GNTP (n=41). Each participant underwent a complete periodontal and peri-implant clinical examination. Collection of saliva samples and radiographic examination to evaluate peri-implant bone levels were conducted at two times: initial examination (T1) and after 5 years (T2). The salivary samples were evaluated through ELISA for the following markers: IL-1β, IL-10, RANK, OPG, MMP-2, TGF and TNF-α. Results A higher incidence of peri-implantitis was observed in the GNTP group (43.9%) than in the GTP group (18%) (p=0.000). All individuals (n=12) who presented peri-implant mucositis and had resolution at T2 were in the GTP group. After 5 years, there was an increase in the incidence of periodontitis in the GNTP group compared to the GTP group (p=0.001). The results of the study revealed an increase in the salivary concentration of TNF-α in the GNTP group compared to the GTP group. The other salivary biomarkers that were evaluated did not show statistically significant differences between the two groups. Conclusions The salivary concentration of TNF-α was increased in individuals with worse periodontal and peri-implant clinical condition and in those with a higher incidence of peri-implantitis, especially in the GNTP group. Longitudinal studies in larger populations are needed to confirm these findings and elucidate the role of this biomarker in peri-implant disease.
Descritores: Periodontite/patologia
Saliva/química
Estomatite/patologia
Implantes Dentários/efeitos adversos
Citocinas/análise
Receptor Ativador de Fator Nuclear kappa-B/análise
Osteoprotegerina/análise
-Periodontite/diagnóstico
Valores de Referência
Estomatite/diagnóstico
Ensaio de Imunoadsorção Enzimática
Biomarcadores/análise
Estudos de Casos e Controles
Fatores de Risco
Seguimentos
Estatísticas não Paramétricas
Progressão da Doença
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1055467
Autor: Mo, Hui; Zhang, Ning; Li, Huifu; Li, Fan; Pu, Rong.
Título: Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;52(12):e8754, 2019. tab, graf.
Idioma: en.
Resumo: Cuscuta chinensis Lam. (Convolvulaceae) is an important herbal medicine widely used to improve sexual function, treat osteoporosis, and prevent aging, and has been reported to exhibit anti-osteoporotic effects in vitro. However, the activity of Cuscuta chinensis Lam. on glucocorticoid-induced osteoporosis still remains unclear. The present study aimed to assess the protective effect and the underlying mechanism of action of Cuscuta chinensis extract (CCE) against glucocorticoid-induced osteoporosis in vivo. Sprague-Dawley rats were randomly divided into four groups as follows: control group, osteoporosis group, and 2 CCE-treated osteoporosis groups (100 mg·kg-1·day-1). Blood samples and femur bones were collected for immunohistochemistry, biochemical, mRNA expression, and western blot analysis. HPLC analysis revealed that chlorogenic acid, quercetin, and hyperin were the major constituents of CCE. The results indicated that CCE increased bone length, bone weight, and bone mineral density and suppressed dexamethasone (DEX)-induced reduction in body weight. In addition, TRAP staining indicated that CCE reduced osteoclasts in DEX-induced osteoporosis rats. Mechanistically, CCE treatment alleviated the increase of bone resorption markers and the decline of osteogenic markers, which might be partially mediated by regulation of RANKL/OPG and RunX2 pathways. These results suggest that CCE showed promising effects in the protection against glucocorticoid-induced osteoporosis through protecting osteoblasts and suppressing osteoclastogenesis.
Descritores: Osteoporose/prevenção & controle
Dexametasona/farmacologia
Extratos Vegetais/farmacologia
Cuscuta/química
Osteoprotegerina/metabolismo
Glucocorticoides/farmacologia
-Osteoporose/induzido quimicamente
RNA Mensageiro
Imuno-Histoquímica
Densidade Óssea/efeitos dos fármacos
Western Blotting
Cromatografia Líquida de Alta Pressão
Ratos Sprague-Dawley
Ligante RANK/efeitos dos fármacos
Ligante RANK/metabolismo
Osteoprotegerina/efeitos dos fármacos
Limites: Animais
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-984039
Autor: Zheng, Lei; Yang, Lixia; Zhao, Xin; Long, Niya; Li, Peifan; Wang, Yiming.
Título: Effect of risperidone on proliferation and apoptosis of MC3T3-E1 cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;52(3):e8098, 2019. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Guizhou Provincial Science and Technology Department; . Platform for Talent of Guizhou.
Resumo: This aim of this study was to assess the molecular mechanism of osteoporosis in schizophrenia patients with risperidone use. Here, we investigated the effects of risperidone on cellular proliferation and apoptosis of a preosteoblast cell line, MC3T3-E1. Cell viability and apoptotic rate of MC3T3-E1 were detected by cell counting kit-8 and flow cytometry at a serial dose of risperidone and at different time points, respectively. Bone transformation relevant gene serum osteocalcin (BGP), collagen 1, tumor necrosis factor-α (TNF-α), osteoprotegerin (OPG), and receptor activator of nuclear factor-κB ligand (RANKL) mRNA levels were determined by real-time PCR (qPCR). Their protein expression patterns were evaluated using western blot. The results revealed that risperidone dramatically inhibited MC3T3-E1 cell proliferation in a dose-dependent manner. It also significantly induced MC3T3-E1 cell apoptosis. TNF-α gene and protein levels were greatly enhanced after risperidone treatment. In contrast, BGP, collagen 1, OPG, and RANKL gene and protein levels were markedly downregulated. Our study indicated that risperidone suppressed MC3T3-E1 cell proliferation and induced apoptosis. It also regulated BGP gene and protein expression.
Descritores: Osteoblastos/efeitos dos fármacos
Apoptose/efeitos dos fármacos
Risperidona/farmacologia
Proliferação de Células/efeitos dos fármacos
-Osteocalcina/efeitos dos fármacos
Linhagem Celular
Colágeno/efeitos dos fármacos
Fator de Necrose Tumoral alfa/efeitos dos fármacos
Receptor Ativador de Fator Nuclear kappa-B/efeitos dos fármacos
Osteoprotegerina/efeitos dos fármacos
Citometria de Fluxo
Limites: Animais
Responsável: BR1.1 - BIREME


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Rivero, Elena Riet Correa
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Id: biblio-974450
Autor: Martini, Georgia; Capella, Diogo; Rivero, Elena Riet Correa; Gondak, Rogério Oliveira.
Título: Immunohistochemical expression of RANKL in oral giant cell lesions is predictive of aggressiveness
Fonte: Braz. oral res. (Online);32:e115, 2018. tab, graf.
Idioma: en.
Projeto: National Council for Scientific and Technological Development.
Resumo: Abstract The aim of this study was to evaluate the immunohistochemical expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and of osteoprotegerin (OPG), important proteins correlated with osteoclastogenesis, in central giant cell lesions (CGCL) and peripheral giant cell lesions (PGCL) and to compare their expression with the histological and clinical parameters for quantification of multinucleated giant cells (MGC) and their nuclei, lesion size, and recurrences. Twenty cases of each lesion type were selected to quantify the number of MGCs and nuclei/mm2 of connective tissue. The immunoreactivity of RANKL and OPG was expressed as a percentage of the marked area in the stroma. Clinical data were collected from pathoanatomical and medical reports. No statistical differences were found for the number of MGCs (p = 0.24) between PGCL and CGCL, but the number of nuclei within the MGCs was higher in CGCL (p = 0.01). RANKL expression was higher in CGCL than in PGCL (p = 0.04) and all recurrent lesions showed higher RANKL and OPG expressions than nonrecurrent lesions. We report higher RANKL expression and a greater number of nuclei in CGCL, which may explain the difference in clinical behaviour between these lesions and their pathogenesis.
Descritores: Granuloma de Células Gigantes/patologia
Doenças Maxilomandibulares/patologia
Células Gigantes/patologia
Ligante RANK/análise
Osteoprotegerina/análise
-Valores de Referência
Imuno-Histoquímica
Valor Preditivo dos Testes
Estudos Retrospectivos
Estatísticas não Paramétricas
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1089383
Autor: Cirano, Fabiano Ribeiro; Pimentel, Suzana Peres; Ribeiro, Fernanda Vieira; Casati, Marcio Zaffalon; Casarin, Renato Corrêa; Gallafasi, Daniel Freire; Nishii, Denise; Corrêa, Mônica Grazieli.
Título: Impact of history of periodontitis on gene expression of bone-related factors in young patients
Fonte: Braz. oral res. (Online);34:e014, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Although dental implants and bone regenerative procedures are important approaches for the reestablishment of esthetics and function in young patients with a history of generalized aggressive periodontitis (GAP), no predictable outcomes have been reported, and the host osteo-immunoinflammatory response may play a relevant role in this context. In view of the lack of molecular investigations into the bone tissue condition of young patients with periodontitis, the aim of this study was to evaluate the gene expression of bone-related factors in this population. Bone biopsies were obtained from the posterior mandible in 16 individuals previously diagnosed with GAP and on periodontal support therapy and from 17 periodontally healthy (PH) patients. The gene expression of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), osteocalcin (OC), bone sialoprotein (BSP), and type I collagen (COL-I), important biomarkers of bone turnover, was evaluated by qRT-PCR. Lower TGF-β and OPG mRNA levels were observed in GAP patients compared to PH individuals (p ≤ 0.05). There were no between-group differences in levels of TNF-α, BSP, RANKL, OC, or COL-I mRNA (p>0.05). In young adults, a history of periodontal disease can negatively modulate the gene expression of important bone-related factors in alveolar bone tissue. These molecular outcomes may contribute to the future development of therapeutic approaches to benefit bone healing in young patients with history of periodontitis via modulation of osteo-immuno-inflammatory biomarkers.
Descritores: Periodontite Agressiva/genética
Expressão Gênica
-Periodontite Agressiva/metabolismo
Valores de Referência
Biomarcadores
Osteocalcina/análise
Osteocalcina/genética
Método Simples-Cego
Estudos Transversais
Fator de Crescimento Transformador beta/análise
Fator de Crescimento Transformador beta/genética
Fator de Necrose Tumoral alfa/análise
Fator de Necrose Tumoral alfa/genética
Estatísticas não Paramétricas
Colágeno Tipo I/análise
Colágeno Tipo I/genética
Ligante RANK/análise
Ligante RANK/genética
Osteoprotegerina/análise
Osteoprotegerina/genética
Sialoproteína de Ligação à Integrina/análise
Sialoproteína de Ligação à Integrina/genética
Processo Alveolar/química
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Masculino
Feminino
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-1135564
Autor: Alida, Alida; Winoto, Ervina Restiwulan; Narmada, Ida Bagus.
Título: Receptor Activator of Nuclear Factor-Kappa Ligand and Osteoprotegerin Expressions on Hyperglycemic Wistar Rats (Rattus Novergicus) During Orthodontic Tooth Movement
Fonte: Pesqui. bras. odontopediatria clín. integr;20:e0022, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Objective: To investigate the differences of receptor activator of nuclear factor-κB ligand (RANKL) and Osteoprotegerin (OPG) expressions between normoglycemic and hyperglycemic Wistar rats (Rattus Novergicus) during Orthodontic Tooth Movement (OTM). Material and Methods: This study was true experimental with post-test group only. Thirty-two healthy male Wistar rats, weighted around 200-250 grams, 12-20 weeks old, were used as OTM animal study. They were divided into 2 groups (n=16), normoglycemic rats (normal blood glucose 80-120 mg/dl) and hyperglycemic rats (>250 mg/dl) induced by Streptozotocin with a dose of 30 mg in PBS injection intraperitoneally. A NiTi closed coil spring was mounted between maxillary first molar and incisors with the light force 10gf/mm2 in both groups to induce OTM. The studied animals were then terminated on days 1, 3, 6, and 9, respectively, and premaxilla was extracted. RANKL and OPG expression were examined utilizing immunohistochemistry (IHC) analysis. One-way ANOVA and Tukey HSD (p<0.05) were utilized to analyze the differences in the expression of RANKL and OPG between groups. Results: The hyperglycemic group on day 1, 9 rats showed a significant increase in the expression of RANKL, whereas OPG expression decreased significantly on days 1, 3, and 9. Conclusion: There was a significant increase of RANKL expression and a decrease of OPG expression in hyperglycemic rats as documented immunohistochemically.
Descritores: Técnicas de Movimentação Dentária
Ratos Wistar
Estreptozocina
Diabetes Mellitus
Ligante RANK
Hiperglicemia
-Imuno-Histoquímica
Epidemiologia Experimental
Análise de Variância
Técnicas de Pesquisa
Osteoprotegerina
Dente Molar
Limites: Animais
Ratos
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos



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