Base de dados : LILACS
Pesquisa : D12.776.543.750.705.852.760.949.249 [Categoria DeCS]
Referências encontradas : 49 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 5 ir para página              

  1 / 49 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1135564
Autor: Alida, Alida; Winoto, Ervina Restiwulan; Narmada, Ida Bagus.
Título: Receptor Activator of Nuclear Factor-Kappa Ligand and Osteoprotegerin Expressions on Hyperglycemic Wistar Rats (Rattus Novergicus) During Orthodontic Tooth Movement
Fonte: Pesqui. bras. odontopediatria clín. integr;20:e0022, 2020. tab, graf.
Idioma: en.
Resumo: Abstract Objective: To investigate the differences of receptor activator of nuclear factor-κB ligand (RANKL) and Osteoprotegerin (OPG) expressions between normoglycemic and hyperglycemic Wistar rats (Rattus Novergicus) during Orthodontic Tooth Movement (OTM). Material and Methods: This study was true experimental with post-test group only. Thirty-two healthy male Wistar rats, weighted around 200-250 grams, 12-20 weeks old, were used as OTM animal study. They were divided into 2 groups (n=16), normoglycemic rats (normal blood glucose 80-120 mg/dl) and hyperglycemic rats (>250 mg/dl) induced by Streptozotocin with a dose of 30 mg in PBS injection intraperitoneally. A NiTi closed coil spring was mounted between maxillary first molar and incisors with the light force 10gf/mm2 in both groups to induce OTM. The studied animals were then terminated on days 1, 3, 6, and 9, respectively, and premaxilla was extracted. RANKL and OPG expression were examined utilizing immunohistochemistry (IHC) analysis. One-way ANOVA and Tukey HSD (p<0.05) were utilized to analyze the differences in the expression of RANKL and OPG between groups. Results: The hyperglycemic group on day 1, 9 rats showed a significant increase in the expression of RANKL, whereas OPG expression decreased significantly on days 1, 3, and 9. Conclusion: There was a significant increase of RANKL expression and a decrease of OPG expression in hyperglycemic rats as documented immunohistochemically.
Descritores: Técnicas de Movimentação Dentária
Ratos Wistar
Estreptozocina
Diabetes Mellitus
Ligante RANK
Hiperglicemia
-Imuno-Histoquímica
Epidemiologia Experimental
Análise de Variância
Técnicas de Pesquisa
Osteoprotegerina
Dente Molar
Limites: Animais
Ratos
Responsável: BR1264.1 - Biblioteca Setorial Prof Alberto M Campos


  2 / 49 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1222633
Autor: Silva, Érica Fernanda Patricio da.
Título: Avaliação clínica, morfológica e molecular do carcinoma epidermoide oral com envolvimento mandibular / Clinical, morphological and molecular evaluation of oral squamous cell carcinoma with mandibular invasion.
Fonte: São Paulo; s.n; 2021. 69 p. ilust, tabelas.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: O carcinoma epidermóide (CEC) é a neoplasia maligna mais frequente em cavidade oral, representando aproximadamente 90% de todos os cânceres da boca. Esta neoplasia tem origem no epitélio de revestimento bucal, e a depender do subtipo e da sua localização, pode apresentar tendência marcante de invasão do tecido ósseo adjacente. Existe uma interação entre osso e neoplasia, e atenção principal deve ser dada aos reguladores relacionados à osteoclastogênese. As moléculas RANK/RANKL/OPG são fundamentais neste processo, e juntamente com a IL-6, promovem formação e ativação de osteoclastos, com consequente promoção da invasão mandibular. Neste sentido, o objetivo do estudo foi avaliar as características clínicas, radiográficas e histopatológicas dos pacientes submetidos a mandibulectomia para tratamento do carcinoma epidermóide oral, e correlacionar/associar com a expressão gênica na neoplasia de marcadores reguladores da invasão óssea. Foi realizado um estudo retrospectivo de pacientes tratados com algum tipo de mandibulectomia no A.C. Camargo Cancer Center durante o período de1990 a 2015. Um total de 220 casos foi selecionado, e destes, foram extraídos 40 casos, posteriormente submetidos as etapas de análise molecular. A análise molecular foi realizada por PCR quantitativa em tempo real (qPCR), por meio da mensuração de mRNA para os genes estudados, calibrados pelo gene endógeno GAPDH e por um pool de controle composto por RNA não neoplásico. Dos 220 pacientes incluídos na pesquisa, 161 eram do sexo masculino, a idade média foi de 60 anos, e 53,6% eram etilistas e 66,8% tabagistas. A invasão óssea da mandíbula foi observada através do laudo anatomopatológico em 77 pacientes, e foi associada à presença de margens comprometidas/exíguas e a localização da lesão (gengiva inferior, rebordo e região retro-molar). Ao final do estudo, 127 pacientes foram a óbito, e a média de sobrevida foi de 96,92 meses. Parâmetros clínicos, dos exames de imagem e histopatológicos foram significantemente associados com a sobrevida global, principalmente - tamanho do tumor, infiltração perineural, invasão óssea (imagem e anatomopatológico). Na análise multivariada, apenas invasão óssea e infiltração perineural aumentaram risco de óbito nos pacientes. Na análise molecular de expressão gênica foi possível avaliar que das 36 amostras do gene RANK, 16 eram downreguladas. Para RANKL, das 37 amostras, 28 estavam super expressas. Das 35 do gene da IL-6, 19 amostras eram downreguladas. E por fim, das 37 amostras do gene da OPG, 16 tinham expressão normal. A avaliação de associação entre a expressão dos genes analisados demostrou que o gene da IL-6 está associado a presença de invasão óssea, e que existe uma associação significativa entre as expressões do RANK, RANKL, OPG e IL-6. Maior sobrevida dos pacientes foi associada significativamente a uma expressão mais baixa de IL-6 (108,5 meses). Conclui-se que a invasão mandibular interfere na sobrevida global dos pacientes, e que este mecanismo de invasão óssea está associado com a expressão gênica dos genes RANK, RANKL, OPG e IL-6. A IL-6 downregulada nas células malignas está associada com maior sobrevida

Oral Squamous Cell Carcinoma (OSCC) is the most frequent malignant neoplasm in the oral cavity, representing 90% of all oral cancers. The tumor usually arises within the epithelium lining of the oral cavity, and in cases of certain subtype or location may reveal a tendency for invasion of the surrounding bone tissue. There is a relation between tumor and bone, and more attention should be given to the events related to osteoclastogenesis. The molecules RANK/RANKL/OPG are crucial in this process, and together with IL-6, promotes the formation and activation of osteoclasts, with influence on the mandibular invasion process. In this way, the aim of this study was to evaluate the clinical, demographic, imaging, and histopathology data of patients diagnosed with OSCC treated by mandibulectomy. Also, analyze the gene expression condition of the genes RANK, RANKL, OPG, and IL-6 on the tumor. A retrospective study was conducted on patients treated with mandibulectomy at the A.C. Camargo Cancer Center, between 1990 and 2015. Two hundred and twenty cases were retrieved, and 40 of them were selected for gene expression analysis. The mRNA expressions of the selected genes were determined by real-time polymerase chain reaction, extracted from the tumor, and calibrated by endogenous GAPDH and a control pool of non-neoplastic RNA. Among 220 patients, 161 were male, with a mean age of 60 years. 53.6% were alcohol users and 66.8% tobacco users. Mandible bone invasion was reported on 77 histopathology reports and associated with compromised margins and the site of the lesion (gingiva, alveolar ridge, and retromolar region). One hundred and twenty-seven patients died of the disease, and the mean overall survival rate was 96.92 months. Overall survival was associated with clinical, imaging, and pathological parameters, especially, tumor size, perineural growth, and bone invasion (both imaging and pathologic). Multivariate analysis revealed higher risk of death only to bone invasion and perineural growth. Gene expression analysis revealed - 16 downregulation of 36 RANK samples evaluated; 28 overexpression of 37 RANKL samples evaluated; 19 downregulation of 35 IL-6 analyzed, and 16 normal expression of 37 OPG samples evaluated. Association analysis revealed that IL-6 expression is associated with bone invasion, and there is also a significant association among the expression of RANK, RANKL, OPG, and IL-6. Higher overall survival rate was associated with an IL-6 down expression (108;5 months). In conclusion, mandibular invasion interferes with the patient's overall survivall rate, and the bone invasion mechanism is associated with gene expression of RANK, RANKL, OPG, and IL-6 on the tumor. IL-6 in downregulation expressed by malignant cells is associated with higher overall survivor rates
Descritores: Prognóstico
Carcinoma de Células Escamosas
Interleucina-6
Receptor Ativador de Fator Nuclear kappa-B
Osteoprotegerina
Mandíbula
Limites: Humanos
Feminino
Gravidez
Adulto
Pessoa de Meia-Idade
Responsável: BR30.1 - Biblioteca
BR30.1


  3 / 49 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1151683
Autor: Braune, André da Silveira.
Título: Anquilose da articulação temporomandibular na infância: análise da expressão gênica da osteoprotegerina e do ligante do fator ativador do receptor nuclear kappa-b / Temporomandibular joint ankylosis in childhood: analysis of osteoprotein gene expression and kappa-b nuclear receptor activator factor ligand.
Fonte: Rio de Janeiro; s.n; 2017. 86 p. ilus, graf.
Idioma: pt.
Tese: Apresentada a Instituto Nacional de Traumatologia E Ortopedia Jamil Haddad. Divisão de ensino e pesquisa. Área de ensino para obtenção do grau de Doutor.
Resumo: A anquilose da articulação temporomandibular (AATM) apesar de rara, é uma doença grave e de difícil tratamento, que restringe os movimentos articulares acarretando ao paciente problemas estéticos, nutricionais, psicológicos e sociais. A formação do bloco ósseo anquilótico determina a gravidade da doença. O remodelamento ósseo é um mecanismo coordenado e extremamente complexo responsável pela formação e pela reabsorção óssea, sendo necessário no reparo de trauma ósseo e na manutenção da homeostasia do metabolismo mineral. Assim, entender o processo de remodelamento ósseo envolvido na fisiopatologia das AATMs pode trazer perspectivas no tratamento desta doença. O objetivo do estudo foi avaliar o perfil de expressão dos genes envolvidos no remodelamento ósseo, osteoprotegerina (OPG) e do fator ativador do receptor nuclear kappa-B/ligante (RANKL), em amostras de crianças com AATM para verificar se existe associação com a degeneração articular da ATM. Foram incluídos no estudo 11 crianças com diagnóstico de AATM e 5 amostras de osso controle (sem alteração patológica na ATM). A idade média dos pacientes com AATM foi de 12 anos, sendo que 9 (82%) já haviam realizado cirurgia prévia de AATM, 36% (n = 4) apresentaram otite em fase neonatal e 18% (n = 2) infecção prévia. A expressão de OPG e RANKL foi avaliada pela reação em cadeia da polimerase quantitativa. Foi observado uma menor expressão de OPG e RANKL nas amostras de pacientes com AATM (bloco anquilótico e osso adjacente à anquilose) comparado as amostras controle. O RANKL e OPG regulam a reabsorção óssea por estimulação do RANK em células precursoras de osteoclastos. Nossos resultados são corroborados por um estudo recente, justificando a investigação da via RANK-RANKL-OPG na AATM, com o intuito de se explorar novas possibilidades terapêuticas, que possam auxiliar no tratamento desta doença que ainda é negligenciada. A menor expressão de OPG e RANKL indicam que a patogênese da AATM em crianças está relacionada a diminuição da reabsorção óssea.

Temporomandibular joint ankylosis (TMJA), although rare, is a serious and difficult to treat disease, which restricts joint movements, causing aesthetic, nutritional, psychological and social problems to the patient. The formation of the ankylotic bone block determines the severity of the disease. Bone remodeling is a coordinated and extremely complex mechanism responsible for bone formation and resorption, and is necessary for the repair of bone trauma and the maintenance of homeostasis of mineral metabolism. Thus, understanding the process of bone remodeling involved in the pathophysiology of TMJA may bring perspectives in the treatment of this disease. The aim of the study was to evaluate the expression profile of the genes involved in bone remodeling, osteoprotegerin (OPG) and nuclear kappa-B receptor activator / RANKL (RANKL) in samples from children with TMJA to verify if there is association with Joint degeneration of TMJ. The study included 11 children with TMJA diagnosis and 5 control bone samples (without pathological alterations in TMJ). The mean age of patients with TMJA was 12 years, and 9 (82%) had previously performed TMJA surgery, 36% (n = 4) presented otitis in the neonatal phase and 18% (n = 2) had previous infection. The expression of OPG and RANKL was evaluated by the quantitative polymerase chain reaction. Lower expression of OPG and RANKL was observed in the samples of patients with TMJA (ankylotic block and bone adjacent to ankylosis) compared to the control samples. RANKL and OPG regulate bone resorption by stimulating RANK in osteoclast precursor cells. Our results are corroborated by a recent study, justifying the investigation of the RANK-RANKL-OPG pathway in the TMJA, in order to explore new therapeutic possibilities that may help in the treatment of this disease that is still neglected. Lower expression of OPG and RANKL indicate that pathogenesis of TMJA in children is related with lower bone resorption.
Descritores:
Articulação Temporomandibular/patologia
Osteoprotegerina
Limites: Humanos
Responsável: BR1756.1 - Sergio Eduardo Vianna
BR1756.1; WU 100 (NLM), B825a


  4 / 49 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-1104226
Autor: Pavlov, Sergey; Babenko, Nataliia; Kumetchko, Marina; Litvinova, Olga; Semko, Natalia; Pavlova, Olga.
Título: Intercellular mediators in bone remodeling regulation in the experimental renal pathology / Mediadores intercelulares de la regulación de la remodelación ósea en un modelo experimental de patología renal
Fonte: Actual. osteol;15(3):180-191, Sept-Dic. 2019. ilus.
Idioma: en.
Resumo: Bone metabolism disorders are characterized by an imbalance of bone resorption and formation in the bone remodeling process. Glucocorticoids that are used to treat kidney diseases exacerbate these disorders. P-selectin and galectin-3 are molecules involved in the sclerotic process in kidney, whereas bone resorption is regulated by the interaction between the nuclear factor activator kappa b receptor (RANK), its ligand (RANKL) and the RANKL decoy receptor osteoprotegerin (OPG). The aim of this study was to investigate the cellular and molecular mechanisms of disruption of bone remodeling regulation processes, reflected by intercellular mediators (RANKL, OPG, P-selectin and galectin-3) in chronic kidney disease experimental model treated with glucocorticoids. Rats were divided into four groups of 10 animals each. The first group, the control group, included intact animals. The second group consisted of rats with impaired bone remodeling resulting from chronic kidney disease (experimental group (CKD). The third group was a group of animals with impaired bone remodeling due to exposure to glucocorticoids (experimental group (GCs)). The fourth group consisted of rats with impaired bone remodeling in chronic kidney disease, followed by exposure to glucocorticoids (experimental group (CKD + GCs)). The effects of CKD and glucocorticoid were evaluated biochemically, histologically and by measuring bone density. An enzymelinked immunoassay was used to measure intercellular mediator levels in the serum. The bone density in the experimental groups was reduced compared to the control group. RANKL levels in animals of three experimental groups were higher than in intact animals. Serum levels of OPG were higher in CKD and GCs groups than in intact animals. At the same time, in the animals' blood serum of the CKD + GCs group, the levels of OPG were lower, than those in animals from the control group. The levels of galectin-3 in the serum of the experimental groups GCs and CKD + GCs were lower than in intact animals. The serum levels of galectin-3 in animals of the CKD group were higher than those in animals from the control group. The levels of P-selectin were lower in the serum of the GCs group than in intact animals. At the same time, the levels of P-selectin were higher in the CKD and CKD + GCs groups, than those in animals from the control group. In conclusion, the study of the complex system of bone remodeling regulation, which includes many factors and their interactions, may lead to the development of new methods for treating patients with chronic kidney disease in order to prevent osteoporosis in the future. (AU)

Las enfermedades metabólicas óseas se caracterizan por un desequilibrio en el proceso de remodelación ósea en los que participan mediadores tales como receptor del activador del factor nuclear- kappa- b (RANK), su ligando (RANKL) y la osteoprotegerina (OPG). Los glucocorticoides, recuentemente empleados en el tratamiento de la enfermedad renal crónica, exacerban este desequilibrio. En la enfermedad esclerótica renal, las moléculas de adhesión celular P-selectina and galectina-3 tienen un rol fundamental. El objetivo de esta trabajo fue estudiar las alteraciones en los mediadores de la remodelación ósea (RANKL, OPG, P-selectina and galectina-3) en un modelo de enfermedad renal crónica con tratamiento glucocorticoideo. Ratas Wistar hembras fueron divididos en 4 grupos: control (C); enfermedad renal crónica con afección de la remodelación ósea (ERC); animales con afección de la remodelación ósea expuestos a glucocorticoides (GC); enfermedad renal crónica con afección de la remodelación ósea tratados con glucocorticoides (ERC+GC). Los efectos de la ERC y los GC fueron evaluados bioquímicamente, histológicamente y por medición de la densidad ósea. RANKL, OPG, Pselectina and galectina-3 se cuantificaron en muestras de sangre venosa empleando enzimoinmuno análisis. En los 3 grupos experimentales la densidad ósea se evidenció reducida y los niveles séricos de RANKL elevados respecto al grupo control. Los niveles de OPG en los grupos ERC y GC fueron superiores mientras que en el grupo ERC+GC menores respecto a los animales controles. Galectina 3 plasmática en GC y ERC+GC se encontró reducida y aumentada en los animales ERC, en comparación con los animales controles. La concentración sérica de P-selectina sérica fue mayor en los grupos ERC y ERC+GC, y menor en los animales GC respecto a los niveles plasmáticos de los animales intactos. El avance del conocimiento sobre la regulación de la remodelación ósea a través de la interacción de mediadores sistémicos, en un futuro, puede conducir al desarrollo de nuevas estrategias terapéuticas para la prevención de la osteoporosis en pacientes con enfermedad renal crónica. (AU)
Descritores: Distúrbio Mineral e Ósseo na Doença Renal Crônica/induzido quimicamente
Remodelação Óssea/efeitos dos fármacos
Nefropatias/fisiopatologia
-Osteoporose/prevenção & controle
Doenças Ósseas Metabólicas/diagnóstico
Dexametasona/administração & dosagem
Densidade Óssea/efeitos dos fármacos
Clorofórmio/uso terapêutico
Ratos Wistar
Selectina-P/efeitos dos fármacos
Selectina-P/sangue
Galectina 3/efeitos dos fármacos
Galectina 3/sangue
Ligante RANK/efeitos dos fármacos
Ligante RANK/sangue
Osteoprotegerina/efeitos dos fármacos
Osteoprotegerina/sangue
Glucocorticoides/efeitos adversos
Glicerol/administração & dosagem
Nefropatias/tratamento farmacológico
Limites: Animais
Ratos
Tipo de Publ: Ensaio Clínico Controlado Aleatório Veterinário
Responsável: AR2.1 - Biblioteca Central


  5 / 49 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1019970
Autor: Borges, Cristine D Almeida; Ricoldi, Milla Sprone; Messora, Michel Reis; Palioto, Daniela Bazan; Souza, Sérgio Luís Scombatti de; Novaes Júnior, Arthur Belém; Taba Jr, Mario.
Título: Clinical attachment loss and molecular profile of inflamed sites before treatment
Fonte: J. appl. oral sci;27:e20180671, 2019. tab, graf.
Idioma: en.
Projeto: State of São Paulo Foundation (FAPESP); . Coordenação de Nível Superior - Brasil (CAPES).
Resumo: Abstract Objective: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. Methodology: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day −15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-β1 protein and gene expression levels. Results: Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-β1 were also observed in inflamed sites at day −15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. Conclusions: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status.
Descritores: Perda da Inserção Periodontal/patologia
-Periodontite/terapia
Saliva/química
Fatores de Tempo
Biópsia
Biomarcadores/análise
Estudos de Casos e Controles
Citocinas/análise
Líquido do Sulco Gengival/química
Estatísticas não Paramétricas
Metaloproteinase 8 da Matriz/análise
Fator A de Crescimento do Endotélio Vascular/análise
Osteoprotegerina/análise
Reação em Cadeia da Polimerase em Tempo Real
Gengiva/patologia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


  6 / 49 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Silva, Léa Assed Bezerra da
Texto completo
Id: biblio-1040231
Autor: Andrucioli, Marcela Cristina Damião; Matsumoto, Mírian Aiko Nakane; Fukada, Sandra Yasuyo; Saraiva, Maria Conceição Pereira; Bergamo, Ana Zilda Nazar; Romano, Fábio Lourenço; Silva, Raquel Assed Bezerra da; Silva, Lea Assed Bezerra da; Nelson-Filho, Paulo.
Título: Quantification of pro-inflammatory cytokines and osteoclastogenesis markers in successful and failed orthodontic mini-implants
Fonte: J. appl. oral sci;27:e20180476, 2019. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation - Process number.
Resumo: Abstract Objectives: Miniscrew has been frequently used, considering that anchorage control is a critical point in orthodontic treatment, and its failure, the main adverse problem. Using two groups of stable (successful) and unstable (failed) mini-implants, this in vivo study aimed to quantify proinflammatory cytokines IL-1 α, IL-6, IL-17, and TNF-α and osteoclastogenesis marker RANK, RANKL, and OPG in gingival tissue, using the real-time polymerase chain reaction technique. Methodology: Thirteen patients of both sexes (11-49 years old) under orthodontic treatment were selected, obtaining 11 successful and 7 failed mini-implants. The mini-implants were placed and removed by the same surgeon, in both jaws. The mean time of permanence in the mouth was 29.4 months for successful and 7.6 months for failed mini-implants. At removal time, peri-mini-implant gingival tissue samples were collected and processed for quantification of the proinflammatory cytokines and osteoclastogenesis markers. Nonparametric Wilcoxon rank-sum test considering the clusters and Kruskal-Wallis test were used for statistical analysis (α=0.05). Results: No significant difference (p>0.05) was observed between the groups for either quantification of cytokines or osteoclastogenesis markers, except for IL-6 (p<0.05). Conclusions: It may be concluded that the expression of IL-1α, IL-17, TNF-α, RANK, RANKL, and OPG in peri-implant gingival tissue were not determinant for mini-implant stability loss, but the higher IL-6 expression could be associated with mini-implant failure.
Descritores: Osteogênese/fisiologia
Implantes Dentários/efeitos adversos
Citocinas/análise
Procedimentos de Ancoragem Ortodôntica/efeitos adversos
Peri-Implantite/patologia
Gengivite/patologia
-Valores de Referência
Fatores de Tempo
Biomarcadores/análise
Perda do Osso Alveolar
Resultado do Tratamento
Estatísticas não Paramétricas
Osteoprotegerina/análise
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


  7 / 49 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1090768
Autor: Ma, Zongmin; Li, Shuxian; Sun, Yuchen.
Título: Effect of enhanced masticatory force on OPG, RANKL and MGF in alveolar bone of ovariectomized rats
Fonte: J. appl. oral sci;28:e20190409, 2020. graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract Menopause induces oral bone loss, leading to various oral diseases. Mastication importantly affects bone metabolism in the jawbone. Objective: To analyze the effect of enhanced masticatory force on osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), and mechano-growth factor (MGF) in alveolar bone of ovariectomized rats and to study the mechanics mechanism of the alveolar bone of ovariectomized rats response to enhanced masticatory force. Methodology: Thirty Sprague Dawley rats were randomly divided into three groups: sham-operation group (fat around the removed ovary + normal hard diet), model group (ovariectomy + normal hard diet), and experimental group (ovariectomy + high hard diet). It was a 2-month experiment. Enzyme-linked immunosorbent assay (ELISA) detected serum estradiol (E2), osteocalcin (BGP) and alkaline phosphatase (ALP) in rats. Bone histomorphometric indices in the third molar region of maxilla were detected by micro-CT; protein expressions of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Western blot; and gene expression of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Quantitative Real-Time PCR. Results: Comparing with model group, serum E2 in experimental group increased but not significantly, serum BGP and serum ALP in experimental group decreased but not significantly, OPG in experimental group in alveolar bone increased significantly, RANKL in experimental group in alveolar bone decreased significantly, RANKL/OPG ratio in experimental group decreased significantly, MGF in experimental group in alveolar bone increased significantly, bone volume to total volume fraction increased significantly in experimental group, trabecular thickness increased significantly in experimental group, and trabecular separation decreased significantly in experimental group. Conclusion: Enhanced masticatory force affected the expression of OPG, RANKL, and MGF in alveolar bone of ovariectomized rats, improved the quality of jaw bone of ovariectomized rats, and delayed oral bone loss by ovariectomy.
Descritores: Força de Mordida
Fator de Crescimento Insulin-Like I/análise
Ovariectomia
Ligante RANK/análise
Osteoprotegerina/análise
Processo Alveolar/fisiopatologia
-Osteocalcina/sangue
Western Blotting
Reação em Cadeia da Polimerase
Ratos Sprague-Dawley
Fosfatase Alcalina/sangue
Estradiol/sangue
Microtomografia por Raio-X
ELISPOT
Limites: Animais
Feminino
Responsável: BR28.1 - Serviço de Biblioteca e Documentação Professor Doutor Antônio Gabriel Atta


  8 / 49 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
Texto completo
Id: biblio-1119580
Autor: Tenório, Jefferson da Rocha.
Título: Avaliação dos níveis séricos e salivares de RANKL, OPG, IL-1?, IL-6 e TNF-? e sua relação com o índice da cortical mandibular em pacientes com cirrose hepática / Evaluation of serum and salivary levels of RANKL, OPG, IL-1?, IL-6 and TNF-? and their association with mandibular cortical index in patients with liver cirrhosis.
Fonte: São Paulo; s.n; 20200000. 81 p.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Odontologia para obtenção do grau de Doutor.
Resumo: Pacientes com cirrose hepática podem desenvolver osteoporose como complicação relacionada ao déficit funcional hepático. O objetivo desse estudo é o de avaliar a presença de proteínas relacionadas ao metabolismo ósseo (RANKL, OPG, IL-1?, IL-6 e TNF-?) na saliva e no sangue de pacientes cirróticos e compará-los com o índice da cortical mandibular (ICM), relacionado à densidade mineral óssea, em radiografias panorâmicas. Trinta e oito pacientes cirróticos foram submetidos à anamnese, exame físico e tiveram amostras de sangue e de saliva não estimulada coletadas. Desses, 22 apresentavam radiografias panorâmicas que foram avaliadas segundo o ICM. As proteínas foram avaliadas através da tecnologia LuminexTM xMAP. Foram considerados estatisticamente significativos valores de p <=0.05. A maioria dos pacientes era do sexo masculino (68,4%), com idade média de 50,61 anos, média de MELD de 18 pontos no momento da consulta e média de 21,34 meses na fila de espera para o transplante. A análise das radiografias panorâmicas permitiu constatar que a maior parte da amostra apresentava alguma modificação na cortical mandibular sugestiva de alterações osteoporóticas (72,7%). O perfil de expressão das proteínas foi bastante variável no sangue e na saliva. Correlação estatisticamente significativa foi observada entre a expressão salivar de RANKL e OPG em pacientes com ICM alterado. Não foi observada relação estatisticamente significante entre o perfil das proteínas e a etiologia da cirrose, tempo na fila de transplante hepático, idade e sexo dos pacientes. As proteínas RANKL, OPG, IL-1?, IL-6 e TNF-? se comportam de maneira diferente na saliva e no sangue. Valores elevados de RANKL e OPG em saliva estão relacionados com ICM sugestivo de osteoporose em pacientes cirróticos, e propõe-se que esse aumento ocorra devido ao microambiente inflamatório local.
Descritores: Osteoporose
Radiografia Panorâmica
Ligante RANK
Osteoprotegerina
Inflamação
-Cirrose Hepática
Responsável: BR97.1 - Serviço de Documentação Odontológica


  9 / 49 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-886206
Autor: Zhang, Chun; Liao, Qi; Ming, Jiang-Hua; Hu, Ge-Liang; Chen, Qing; Liu, Shi-Qing; Li, Ya-Ming.
Título: The effects of chitosan oligosaccharides on OPG and RANKL expression in a rat osteoarthritis model
Fonte: Acta cir. bras;32(6):418-428, June 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the effect of chitosan oligosaccharides (COS) against osteoarthritis (OA) and preliminarily discuss the osteoprotegerin (OPG), receptor activator of NF-κB ligand (RANKL) and RANK expression in a rat OA model. Methods: Thirty-six 6-week-old Male SD rats were randomly divided into three groups: sham-operated group(CON), OA-induction group(OA), COS intervention group(n=12/group). At 4 weeks after the operation, COS (50 ul) intervention weekily for consecutive 5 weeks. The OA and CON groups received an injection of 50 ul physiological saline. At death, 11 weeks following surgery, cartilage was harvested and total RNA and protein were extracted. Both the morphological changes of the cartilage were observed and harvested the total RNA and protein. Meanwhile, the expression of OPG, RANKL and RANK in cartilage were determined. Results: The expression of OPG and RANKL were both enhanced in the cartilage of the OA model. Compared with the OA group, COS treatment improved the cartilage damage (both extent and grade). Furthermore, the COS group showed highly OPG and lower RANKL. Simultaneously, COS treatment upregulated the ratio of OPG/RANKL and downregulated the RANKL/RANK. Conclusion: Chitosan oligosaccharides may be used as a unique biological agent to prevent and treat osteoarthritis, and this effect is associated with modulation of the expression of osteoprotegerin and receptor activator of NF-κB ligand.
Descritores: Oligossacarídeos/farmacologia
Osteoartrite/metabolismo
Cartilagem Articular/efeitos dos fármacos
Quitosana/farmacologia
Ligante RANK/metabolismo
Osteoprotegerina/metabolismo
-Cartilagem Articular/metabolismo
Regulação da Expressão Gênica
Ratos Sprague-Dawley
Modelos Animais de Doenças
Osteoprotegerina/efeitos dos fármacos
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


  10 / 49 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
Id: biblio-973330
Autor: Sánchez, Ariel; Raggio, Juan Carlos; Valtorta, Emiliano.
Título: Cascada de fracturas vertebrales en paciente osteoporótica al año de suspender Denosumab / Cascade of vertebral fractures in osteoporotic patient one year after Denosumab
Fonte: Rev. med. Rosario;84(1):22-25, ene.-abr. 2018. ilus.
Idioma: es.
Resumo: Una paciente con osteoporosis había sido tratada por 4 años con ibandronato oral, luego por 1 año con ranelato de estroncio, y finalmente por 4 años con denosumab. En vista de la buena respuesta densitométrica este fármaco fue suspendido a fines de 2015. A los 14 meses la enferma tuvo lumbalgia aguda y se detectó hundimiento del platillo superior de L1, a lo que siguieron en rápida sucesión iguales lesiones en L2 y L3, y acuñamiento de D11 y D12. Se descartaron causas de osteoporosis secundaria. El plan terapéutico incluye corsé ortopédico, analgésicos, y teriparatida. En los dos últimos años se han publicado varios casos de este síndrome.

A patient with osteoporosis had been treated for 4 years with oral ibandronate, then for 1 year with strontium ranelate, and finally for 4 years with denosumab. In view of the good densitometric response to the latter, the drug was discontinued in December 2015. Fourteen months later the patient had acute low back pain; crushing of the upper plate of L1 was detected, followed by similar lesions in L2 and L3, and wedging of D11 and D12. Causes of secondary osteoporosis were ruled out. The therapeutic strategy includes a corset, analgesics, and teriparatide. In the last two years several cases of this syndrome have been reported.
Descritores: Anticorpos Monoclonais
Anticorpos Monoclonais/efeitos dos fármacos
Osteoporose Pós-Menopausa/prevenção & controle
Fraturas da Coluna Vertebral/diagnóstico
Fraturas da Coluna Vertebral/prevenção & controle
-Osteoprotegerina
Osteoprotegerina/efeitos dos fármacos
Resultado do Tratamento
Limites: Humanos
Feminino
Idoso de 80 Anos ou mais
Tipo de Publ: Relatos de Casos
Responsável: AR16.1 - Biblioteca



página 1 de 5 ir para página              
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde