Base de dados : LILACS
Pesquisa : D12.776.543.750.705.940 [Categoria DeCS]
Referências encontradas : 3 [refinar]
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Id: biblio-1021049
Autor: Lei, Chao; Hao, Ruijuan; Zheng, Zhe; Deng, Yuewen; Wang, Qingheng; Li, Junhui.
Título: Molecular cloning and characterisation of scavenger receptor class B in pearl oyster Pinctada fuctada martensii
Fonte: Electron. j. biotechnol;30:12-17, nov. 2017. tab, graf.
Idioma: en.
Projeto: Graduate Education Innovation Program of Guangdong Ocean University; . Administration of Ocean and Fisheries of Guangdong Province; . Innovation Strong School Project of Guangdong Province.
Resumo: Background: Molluscs can accumulate carotenoids in their body tissues by predominantly feeding on aquatic plant sources. Carotenoid transport and absorption are determined by the regulation of various proteins such as Scavenger receptor class B(SR-BI). We report the identification and characterisation of pearl oyster Pinctada fuctada martensii SR-BI (PmSR-BI). The correlation between total carotenoid content (TCC) and gene expression was also estimated. Results: The full-length cDNA of PmSR-BI was 1828 bp, including an open-reading frame encoding of 1518 bp with a pI value of 5.83. PmSR-BI protein contains a hydrophobic CD36 domain and four centrally clustered cysteine residues for the arrangement of disulphide bridges. The deduced amino acid sequence had an identity of 30% to 60% with the SR-B of other organisms. Reverse transcription polymerase chain reaction analysis showed that mRNA transcripts were expressed in multiple tissues of adult pearl oyster. A higher expression of PmSR-BI gene was observed in the hepatopancreas than in the adductor muscle, gill and mantle. The TCC and gene expression of PmSR-BI were significantly correlated (P b 0.05), with a correlation coefficient of 0.978. Conclusions: The results suggested that PmSR-BI is involved in the absorption of carotenoids in the pearl oyster P. fuctada martensii.
Descritores: Carotenoides/metabolismo
Receptores Depuradores/genética
Receptores Depuradores/metabolismo
Vitamina A/metabolismo
RNA Mensageiro/genética
Expressão Gênica
Clonagem Molecular
Análise de Sequência
Ácido Abscísico
DNA Complementar/genética
Interações Hidrofóbicas e Hidrofílicas
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Brasil
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Id: lil-624024
Autor: Rosanas-Urgell, Anna; Martin-Jaular, Lorena; Ricarte-Filho, Julio; Ferrer, Mireia; Kalko, Susana; Kimura, Edna; del Portillo, Hernando A.
Título: Expression of non-TLR pattern recognition receptors in the spleen of BALB/c mice infected with Plasmodium yoelii and Plasmodium chabaudi chabaudi AS
Fonte: Mem. Inst. Oswaldo Cruz;107(3):410-415, May 2012. ilus, graf, tab.
Idioma: en.
Projeto: CNPq; . FAPESP; . European Community's Seventh Framework Programme; . Spanish Ministry of Science and Innovation.
Resumo: The spleen plays a crucial role in the development of immunity to malaria, but the role of pattern recognition receptors (PRRs) in splenic effector cells during malaria infection is poorly understood. In the present study, we analysed the expression of selected PRRs in splenic effector cells from BALB/c mice infected with the lethal and non-lethal Plasmodium yoelii strains 17XL and 17X, respectively, and the non-lethal Plasmodium chabaudi chabaudi AS strain. The results of these experiments showed fewer significant changes in the expression of PRRs in AS-infected mice than in 17X and 17XL-infected mice. Mannose receptor C type 2 (MRC2) expression increased with parasitemia, whereas Toll-like receptors and sialoadhesin (Sn) decreased in mice infected with P. chabaudi AS. In contrast, MRC type 1 (MRC1), MRC2 and EGF-like module containing mucin-like hormone receptor-like sequence 1 (F4/80) expression decreased with parasitemia in mice infected with 17X, whereas MRC1 an MRC2 increased and F4/80 decreased in mice infected with 17XL. Furthermore, macrophage receptor with collagenous structure and CD68 declined rapidly after initial parasitemia. SIGNR1 and Sn expression demonstrated minor variations in the spleens of mice infected with either strain. Notably, macrophage scavenger receptor (Msr1) and dendritic cell-associated C-type lectin 2 expression increased at both the transcript and protein levels in 17XL-infected mice with 50% parasitemia. Furthermore, the increased lethality of 17X infection in Msr1 -/- mice demonstrated a protective role for Msr1. Our results suggest a dual role for these receptors in parasite clearance and protection in 17X infection and lethality in 17XL infection.
Descritores: Lectinas Tipo C/imunologia
Lectinas de Ligação a Manose/imunologia
Plasmodium chabaudi/imunologia
Plasmodium yoelii/imunologia
Receptores de Superfície Celular/imunologia
Receptores Depuradores/imunologia
Receptores Toll-Like/imunologia
-Citometria de Fluxo
Lectinas Tipo C/genética
Camundongos Endogâmicos BALB C
Análise em Microsséries
Lectinas de Ligação a Manose/genética
Receptores de Superfície Celular/genética
Receptores Depuradores/genética
Receptores Toll-Like/genética
Limites: Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

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Texto completo SciELO Chile
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Id: lil-443673
Autor: Rigotti, A.
Título: Scavenger receptors and atherosclerosis
Fonte: Biol. Res;33(2):97-103, 2000.
Idioma: en.
Resumo: Scavenger receptors were discovered as cell surface proteins capable of binding and internalization of modified lipoproteins. These receptors exhibit a broad ligand binding specificity including potential physiological and pathophysiological ligands other than modified lipoproteins. Different classes of scavenger receptors have been identified, and their relevance in normal and pathological conditions is under investigation. Recent in vitro and in vivo studies strongly support the role of class A and class B scavenger receptors in lipid transport and atherogenesis.
Descritores: Arteriosclerose/metabolismo
Lipoproteínas LDL/metabolismo
Receptores Imunológicos/metabolismo
-Transporte Biológico
Proteínas de Membrana/metabolismo
Receptores Depuradores
Receptores Depuradores Classe A
Receptores Depuradores Classe B
Limites: Animais
Responsável: BR1.1 - BIREME

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