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Id: biblio-1132742
Autor: Tayman, Mahmure Ayse; Önder, Canan; Kurgan, Sivge; Serdar, Muhittin Abdulkadir; Günhan, Meral.
Título: Endocan (ESM-1) levels in gingival crevicular fluid correlate with ICAM-1 and LFA-1 in periodontitis
Fonte: Braz. oral res. (Online);35:e005, 2021. tab, graf.
Idioma: en.
Projeto: Ankara University Scientific Research Projects Office.
Resumo: Abstract: Endocan, a 50 kDa soluble proteoglycan, also called endothelial cell-specific molecule-1 (ESM-1), is involved in many major cellular activities and has been reported to be overexpressed in inflammatory conditions. This study aims to determine ESM-1 levels in gingival crevicular fluid (GCF) samples from individuals with periodontitis to determine the correlation between the levels of lymphocyte-function-associated antigen-1 (LFA-1), intercellular adhesion molecule-1 (ICAM-1), and clinical findings of periodontitis. This study enrolled 27 individuals diagnosed with Stage III-Grade C generalized periodontitis and 16 individuals as healthy controls. Bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment loss (CAL) were calculated. Enzyme-linked immunosorbent assay (ELISA) test was used for detecting the levels of ESM-1, ICAM-1, and LFA-1 in GCF samples. PPD, BOP, CAL, and GCF volumes were significantly increased in patients with periodontitis in comparison to the control group (p < 0.001). The total amount of ESM-1, ICAM-1, and LFA-1 levels in GCF were increased in the periodontitis group (p < 0.001). ESM-1 level correlated with PPD, BOP, and CAL (p < 0.05). ICAM-1 level correlated with BOP and CAL (p < 0.05). LFA-1 level correlated with PPD and CAL (p < 0.05). Our data indicate that ESM-1, ICAM-1, and LFA-1 levels are increased in GCF of patients with periodontitis. These molecules could be associated with the pathogenesis and progression of periodontal disease.
Descritores: Periodontite
Periodontite Crônica
-Proteoglicanas
Ensaio de Imunoadsorção Enzimática
Antígeno-1 Associado à Função Linfocitária
Líquido do Sulco Gengival
Molécula 1 de Adesão Intercelular
Proteínas de Neoplasias
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-973381
Autor: Oliveira, Levindo Alves de; Oshima, Celina Tizuko Fujiyama; Soffner, Pedro Augusto; Silva, Marcelo de Souza; Lins, Rodrigo Rego; Malinverni, Andréa Cristina de Moraes; Waisberg, Jaques.
Título: The canonical wnt pathway in gastric carcinoma / Via canônica do wnt no carcinoma gástrico
Fonte: ABCD arq. bras. cir. dig;32(1):e1414, 2019. tab, graf.
Idioma: en.
Resumo: ABSTRACT Background : It is believed that the Wnt pathway is one of the most important signaling involved in gastric carcinogenesis. Aim : To analyze the protein expression of canonical and non-canonical Wnt pathways in gastric carcinoma. Method : The immunohistochemistry was performed in 72 specimens of gastric carcinomas for evaluating the expression of Wnt-5a, FZD5, GSK3β, axin, CK1, ubiquitin, cyclin D1 and c-myc. Results : There were significant differences for cytoplasm and nucleus ubiquitin for moderately and well differentiated tumors (p=0.03) and for those of the intestinal type of the Lauren classification (p=0.03). The absence of c-myc was related to Lauren's intestinal tumors (p=0.03). Expression of CK1 in the cytoplasm was related to compromised margin (p=0.03). Expression of cyclin D1 protein was more intense in male patients (p=0.03) There was no relation of the positive or negative expression of the Wnt-5a, FZD5, GSK3 and Axin with any clinicopathological variables. Conclusion: The canonical WNT pathway is involved in gastric carcinoma.

RESUMO Racional : Acredita-se que a via Wnt é uma das mais importantes da sinalização envolvidas na carcinogênese gástrica. Objetivos : Analisar a expressão das proteínas das vias Wnt canônicas e não-canônicas no carcinoma gástrico e relacionar sua expressão com as variáveisclinicopatológicas. Método : Foram coletadas 72 amostras de carcinoma gástrico, e áreas representativas do tumor foram selecionadas para o Tissue Microarray. Imunoistoquímica foi realizada para avaliar a expressão de Wnt-5a, FZD5, GSK3β, axina, CK1, ubiquitina, ciclina D1 e c-myc. Resultados : Houve diferenças significativas para a expressão de ubiquitina no citoplasma e núcleo para tumores moderadamente e bem diferenciados (p=0,03) e para aqueles do tipo intestinal da classificação de Lauren (p=0,03). A expressão negativa da proteína c-myc no citoplasma foi relacionada aos tumores intestinais de Lauren (p=0,028). A expressão positiva de CK1 no citoplasma das células neoplásicas foi relacionada a tumores com margens cirúrgicas livre de envolvimento neoplásico (p=0,03). A expressão positiva da proteína ciclina D1 foi maior nos tumores dos homens (p=0,03). Não houve relação da expressão positiva ou negativa das proteínas Wnt-5a e FZD5 no citoplasma ou núcleo com quaisquer variáveis clinicopatológicas. O mesmo foi observado para GSK3β e Axin. Conclusões : A relação da expressão das proteínas da via canônica com as variáveis epidemiológicas e tumorais sugere sua participação na carcinogênese gástrica. Por outro lado, a ausência da relação das expressões das proteínas da via não-canônica sugere sua não participação na carcinogênese gástrica.
Descritores: Neoplasias Gástricas/química
Carcinoma/química
Via de Sinalização Wnt
Proteínas de Neoplasias/análise
-Valores de Referência
Neoplasias Gástricas/patologia
Imuno-Histoquímica
Carcinoma/patologia
Proteínas Proto-Oncogênicas c-myc/análise
Ciclina D1/análise
Ubiquitina/análise
Caseína Quinase I/análise
Receptores Frizzled/análise
Proteína Axina/análise
Carcinogênese
Glicogênio Sintase Quinase 3 beta/análise
Proteína Wnt-5a/análise
Estadiamento de Neoplasias
Limites: Humanos
Masculino
Feminino
Responsável: BR1.1 - BIREME


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Bento, Ricardo Ferreira
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Id: biblio-1039535
Autor: Massucci-Bissoli, Milene; Lezirovitz, Karina; Oiticica, Jeanne; Bento, Ricardo Ferreira.
Título: Evidence of progenitor cells in the adult human cochlea: sphere formation and identification of ABCG2
Fonte: Clinics;72(11):714-717, Nov. 2017. tab, graf.
Idioma: en.
Resumo: OBJECTIVES: The aim of this study was to search for evidence of stem or progenitor cells in the adult human cochlea by testing for sphere formation capacity and the presence of the stem cell marker ABCG2. METHODS: Cochleas removed from patients undergoing vestibular schwannoma resection (n=2) and from brain-dead organ donors (n=4) were dissociated for either flow cytometry analysis for the stem cell marker ABCG2 or a sphere formation assay that is widely used to test the sphere-forming capacity of cells from mouse inner ear tissue. RESULTS: Spheres were identified after 2-5 days in vitro, and the stem cell marker ABCG2 was detected using flow cytometric analysis after cochlear dissociation. CONCLUSIONS: Evidence suggests that there may be progenitor cells in the adult human cochlea, although further studies are required.
Descritores: Células-Tronco/citologia
Cóclea/citologia
Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP/análise
Proteínas de Neoplasias/análise
-Proliferação de Células
Citometria de Fluxo
Limites: Humanos
Masculino
Feminino
Adolescente
Pessoa de Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-952839
Autor: Tamura, Rodrigo Esaki; de Luna, Igor Vieira; Lana, Marlous Gomes; Strauss, Bryan E.
Título: Improving adenoviral vectors and strategies for prostate cancer gene therapy
Fonte: Clinics;73(supl.1):e476s, 2018. graf.
Idioma: en.
Resumo: Gene therapy has been evaluated for the treatment of prostate cancer and includes the application of adenoviral vectors encoding a suicide gene or oncolytic adenoviruses that may be armed with a functional transgene. In parallel, versions of adenoviral vector expressing the p53 gene (Ad-p53) have been tested as treatments for head and neck squamous cell carcinoma and non-small cell lung cancer. Although Ad-p53 gene therapy has yielded some interesting results when applied to prostate cancer, it has not been widely explored, perhaps due to current limitations of the approach. To achieve better functionality, improvements in the gene transfer system and the therapeutic regimen may be required. We have developed adenoviral vectors whose transgene expression is controlled by a p53-responsive promoter, which creates a positive feedback mechanism when used to drive the expression of p53. Together with improvements that permit efficient transduction, this new approach was more effective than the use of traditional versions of Ad-p53 in killing prostate cancer cell lines and inhibiting tumor progression. Even so, gene therapy is not expected to replace traditional chemotherapy but should complement the standard of care. In fact, chemotherapy has been shown to assist in viral transduction and transgene expression. The cooperation between gene therapy and chemotherapy is expected to effectively kill tumor cells while permitting the use of reduced chemotherapy drug concentrations and, thus, lowering side effects. Therefore, the combination of gene therapy and chemotherapy may prove essential for the success of both approaches.
Descritores: Neoplasias da Próstata/terapia
Terapia Genética/métodos
Adenoviridae/genética
Carcinoma Pulmonar de Células não Pequenas/genética
Vetores Genéticos/uso terapêutico
Neoplasias Pulmonares/genética
-Neoplasias da Próstata/genética
Neoplasias da Próstata/imunologia
Proteína Supressora de Tumor p53/biossíntese
Antígeno Prostático Específico/genética
Genes Transgênicos Suicidas
Proteínas de Neoplasias/genética
Limites: Humanos
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


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Id: biblio-950736
Autor: Berenguer, Anabela Gonçalves; Fernandes, Ana Teresa; Oliveira, Susana; Rodrigues, Mariana; Ornelas, Pedro; Romeira, Diogo; Serrão, Tânia; Rosa, Alexandra; Câmara, Rita.
Título: Genetic polymorphisms and asthma: findings from a case - control study in the Madeira island population
Fonte: Biol. Res;47:1-11, 2014. tab.
Idioma: en.
Projeto: FEDER; . Fundação para a Ciência e Tecnologia.
Resumo: BACKGROUND: Asthma is a complex disease influenced by multiple genetic and environmental factors. While Madeira has the highest prevalence of asthma in Portugal (14.6%), the effect of both genetic and environmental factors in this population has never been assessed. We categorized 98 asthma patients according to the Global Initiative for Asthma (GINA) guidelines, established their sensitization profile, and measured their forced expiratory volume in 1second (FEV1) and forced vital capacity (FVC) indexes. Selected single nucleotide polymorphisms (SNPs) were analysed as potential markers for asthma susceptibility and severity in the interleukin 4 (IL4), interleukin 13 (IL13), beta-2-adrenergic receptor (ADRB2), a disintegrin and metalloprotease 33 (ADAM33), gasdermin-like (GSDML) and the signal transducer and activator of transcription 6 (STAT6) genes comparatively to a population reference set. RESULTS: Although mites are the major source of allergic sensitization, no significant difference was found amongst asthma severity categories. IL4-590*CT/TT and IL4-RP2*253183/183183 were found to predict the risk (2-fold) and severity (3 to 4-fold) of asthma and were associated with a lower FEV1 index. ADRB2-c.16*AG is a risk factor (3.5-fold), while genotype GSDML-236*TT was protective (4-fold) for moderate-severe asthma. ADAM33-V4*C was associated to asthma and mild asthma by the transmission disequilibrium test (TDT). Finally, ADAM33-V4*CC and STAT6-21*TT were associated with higher sensitization (mean wheal size ≥10mm) to house dust (1.4-fold) and storage mite (7.8-fold). CONCLUSION: In Madeira, IL4-590C/T, IL4-RP2 253/183, GSDML-236C/T and ADAM33-V4C/G SNPs are important risk factors for asthma susceptibility and severity, with implications for asthma healthcare management.
Descritores: Polimorfismo Genético/genética
Asma/genética
-Portugal
Índice de Gravidade de Doença
Biomarcadores
Estudos de Casos e Controles
Capacidade Vital/genética
Volume Expiratório Forçado/genética
Fatores de Risco
Interleucina-4/análise
Interleucina-4/genética
Receptores Adrenérgicos beta 2/análise
Receptores Adrenérgicos beta 2/genética
Estatísticas não Paramétricas
Interleucina-13/análise
Interleucina-13/genética
Desintegrinas/análise
Desintegrinas/genética
Polimorfismo de Nucleotídeo Único/genética
Proteínas ADAM/análise
Proteínas ADAM/genética
Fator de Transcrição STAT6/análise
Fator de Transcrição STAT6/genética
Genótipo
Proteínas de Neoplasias/análise
Proteínas de Neoplasias/genética
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950790
Autor: Chen, Yijie; Ma, Lin; He, Qian; Zhang, Shaobo; Zhang, Chenghua; Jia, Wei.
Título: TGF-ß1 expression is associated with invasion and metastasis of intrahepatic cholangiocarcinoma
Fonte: Biol. Res;48:1-5, 2015. ilus, graf, tab.
Idioma: en.
Projeto: Military youth support program of China.
Resumo: BACKGROUND: Transforming growth factor (TGF)-ß is involved in many physiologic processes, it often promotes metastasis, and its high expression is correlated with poor prognosis. In the present study, we analyzed the correlation between transforming growth factor beta 1 (TGF-ß1) expression and prognosis in intrahepatic cholangiocarcinoma RESULTS: We examined the expression of TGF-ß1 in 78 intrahepatic cholangiocarcinomas by immunohistochemistry and correlated the expression with clinicopathological parameters. TGF-ß1 was expressed in 37 of 78 (47.4 %) intrahepatic cholangiocarcinomas. The expression of TGF-ß1 was significantly correlated with lymph node metastasis, distant metastasis, and tumour recurrence. Patients with TGF-ß1-positive tumours had significantly shorter survival time. In a multivariant analysis, the expression of TGF-ß1 and the tumour stage were independent prognostic factors CONCLUSIONS: Our data suggest that expression of TGF-ß1 is a novel prognostic marker for intrahepatic cholangiocarcinoma.
Descritores: Colangiocarcinoma/metabolismo
Fator de Crescimento Transformador beta1/metabolismo
Neoplasias Hepáticas/metabolismo
Proteínas de Neoplasias/metabolismo
-Fatores de Tempo
Imuno-Histoquímica
Biomarcadores Tumorais/sangue
Colangiocarcinoma/mortalidade
Colangiocarcinoma/patologia
Colangiocarcinoma/secundário
Estimativa de Kaplan-Meier
Neoplasias Hepáticas/mortalidade
Neoplasias Hepáticas/patologia
Metástase Linfática
Estadiamento de Neoplasias
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950816
Autor: Liang, Yangui; Hua, Qiang; Pan, Pengwei; Yang, Jie; Zhang, Qi.
Título: Development of a novel method to evaluate sialylation of glycoproteins and analysis of gp96 sialylation in Hela, SW1990 and A549 cell lines
Fonte: Biol. Res;48:1-9, 2015. ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: BACKGROUND: Glycoproteins play a critical role in the cellular activities of eukaryotes. Sialic acid is typically the outermost monosaccharide of glycolipids and glycoproteins, and is necessary for normal development. RESULTS: A strategy based on avidin-biotin affinity was established to enrich sialylated glycoproteins from HeLa cervical carcinoma, SW1990 pancreatic adenocarcinoma, and A549 lung adenocarcinoma cells. Using HPLC-MS/MS, western blot, real-time PCR, and enzyme-linked immunosorbent assay, gp96 was identified in all three cell lines. No significant difference in the protein expression of gp96 was detected at the whole cell level, but the amount of bioti-nylated gp96 in SW1990 cells was 30-40 % lower than that in A549 and HeLa cells, and the amount of sialylated gp96 in SW1990 cells was 30 % lower than that in A549 and HeLa cells. Immunoblotting results showed that the expression of sialyltransferase proteins in the total cell lysates from HeLa and A549 cells were higher than that in SW1990 cells. CONCLUSIONS: We established a new method for investigating the expression and sialylation of glycoproteins using metabolic labeling, click chemistry, and avidin-biotin affinity. We successfully used this method to purify sialylated glycoproteins from cancer cell lines. Our results showed that the levels of gp96 sialylation varied across different cancer cell lines, and this may be because of differences in sialyltransferase expression.
Descritores: Ácidos Siálicos/metabolismo
Glicoproteínas de Membrana/metabolismo
Glicosiltransferases/metabolismo
Proteínas de Neoplasias/metabolismo
-Ensaio de Imunoadsorção Enzimática
Células HeLa
Espectrometria de Massas em Tandem
Reação em Cadeia da Polimerase em Tempo Real
Células A549
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-950887
Autor: Yuan, Feng; Wang, Junfeng; Zhang, Keshi; Li, Zhao; Guan, Zhenpeng.
Título: Programmed cell death 5 transgenic mice attenuates adjuvant induced arthritis by 2 modifying the T lymphocytes balance
Fonte: Biol. Res;50:40, 2017. graf.
Idioma: en.
Projeto: National Natural Science Fund of China.
Resumo: BACKGROUND: Programmed cell death 5 (PDCD5) is an apoptosis-related gene cloned from TF-1 cells whose primary biological functions are to promote apoptosis and immune regulation. The effects and mechanisms exerted by key mediators of arthritic inflammation remain unclear in PDCD5 transgenic (PDCD5 tg) mice. RESULTS: In the current study, PDCD5 tg mice inhibited the progression of adjuvant-induced arthritis, specifically decreasing clinical signs and histological damage, compared with arthritis control mice. Additionally, the ratio of CD4+IFN-γ+ cells (Th1) and CD4+IL-17A+ cells (Th17), as well as the mRNA expression of the pro-inflammatory mediators IFN-γ, IL-6, IL-17A and TNF-α, were decreased in PDCD5 tg mice, while CD4+CD25+Foxp3+ regulatory T (Treg) cells and the anti-inflammatory mediators IL-4 and IL-10 were increased. Furthermore, PDCD5 tg mice demonstrated reduced serum levels of IFN-γ, IL-6, IL-17A and TNF-α and increased levels of IL-4. CONCLUSIONS: Based on our data, PDCD5 exerts anti-inflammatory effects by modifying the T lymphocytes balance, inhibiting the production of pro-inflammatory mediators and promoting the secretion of anti-inflammatory cytokines, validating PDCD5 protein as a possible treatment for RA.
Descritores: Artrite Experimental/metabolismo
Linfócitos T Reguladores/fisiologia
Proteínas Reguladoras de Apoptose/fisiologia
Proteínas de Neoplasias/fisiologia
-Artrite Experimental/imunologia
Camundongos Transgênicos
Proteínas Reguladoras de Apoptose/genética
Camundongos Endogâmicos C57BL
Proteínas de Neoplasias/genética
Limites: Animais
Masculino
Camundongos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1019497
Autor: Yang, Ju-Hong; Lin, Lie-Kun; Zhang, Song.
Título: Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
Fonte: Biol. Res;52:31, 2019. graf.
Idioma: en.
Projeto: Shenzhen Medical Research Project funding.
Resumo: BACKGROUND: The purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells. METHODS: The levels of methylation and expression of the DACT1 gene in nasopharyngeal carcinoma tissues and CNE2 cells were determined by methylation-specific PCR and RT-PCR, respectively. CNE2 cells were treated with 5-aza-2-deoxycytidine, and the variation in the methylation status of the DACT1 gene was detected, as well as the influence of methylation on invasiveness of nasopharyngeal carcinoma cells. RESULTS: The DACT1 gene was hyper-methylated in 44 of 62 cases of nasopharyngeal carcinoma. The DACT1 gene was hyper-methylated in 32 of 38 cases of nasopharyngeal carcinoma with lymph node metastasis, and the DACT1 gene was hyper-methylated in 7 of 24 cases of nasopharyngeal carcinoma without lymph node metastasis. The DACT1 mRNA level was weakly expressed or not expressed in all nasopharyngeal carcinoma tissues with hyper-methylated DACT1 genes; however, the DACT1 mRNA level was highly expressed in nasopharyngeal carcinoma tissues with low expression of the methylated DACT1 gene. The DACT1 gene was hyper-methylated and not expressed in CNE2 cells that did not have 5-aza-2-deoxycytidine treatment. After 5-aza-2-deoxycytidine treatment, the DACT1 gene was demethylated and the expression of DACT1 was restored. Moreover, the invasion ability was inhibited in CNE2 cells treated with 5-aza-2-deoxycytidine. CONCLUSION: The expression of DACT1 was related to the methylation status. High expression of DACT1 may inhibit the invasion and metastasis of nasopharyngeal carcinoma cells.
Descritores: Proteínas Nucleares/genética
Neoplasias Nasofaríngeas/patologia
Metilação de DNA/genética
Proteínas Adaptadoras de Transdução de Sinal/genética
Carcinoma Nasofaríngeo/secundário
-Proteínas Nucleares/metabolismo
Neoplasias Nasofaríngeas/genética
Regiões Promotoras Genéticas
Metilação de DNA/fisiologia
Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Carcinoma Nasofaríngeo/genética
Invasividade Neoplásica
Proteínas de Neoplasias/metabolismo
Limites: Humanos
Masculino
Feminino
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1019506
Autor: Li, Wei; Chen, Yan; Sun, Xuan; Yang, Jupeng; Zhang, David Y; Wang, Daguang; Suo, Jian.
Título: Protein expression profiles and clinicopathologic characteristics associate with gastric cancer survival
Fonte: Biol. Res;52:42, 2019. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Excellent Young Foundation of Jilin Scientific and Technological Development Program.
Resumo: BACKGROUND: Prognosis remains one of most crucial determinants of gastric cancer (GC) treatment, but current methods do not predict prognosis accurately. Identification of additional biomarkers is urgently required to identify patients at risk of poor prognoses. METHODS: Tissue microarrays were used to measure expression of nine GC-associated proteins in GC tissue and normal gastric tissue samples. Hierarchical cluster analysis of microarray data and feature selection for factors associated with survival were performed. Based on these data, prognostic scoring models were established to predict clinical outcomes. Finally, ingenuity pathway analysis (IPA) was used to identify a biological GC network. RESULTS: Eight proteins were upregulated in GC tissues versus normal gastric tissues. Hierarchical cluster analysis and feature selection showed that overall survival was worse in cyclin dependent kinase (CDK)2, Akt1, X-linked inhibitor of apoptosis protein (XIAP), Notch4, and phosphorylated (p)-protein kinase C (PKC) α/ß2 immunopositive patients than in patients that were immunonegative for these proteins. Risk score models based on these five proteins and clinicopathological characteristics were established to determine prognoses of GC patients. These proteins were found to be involved in cancer related-signaling pathways and upstream regulators were identified. CONCLUSION: This study identified proteins that can be used as clinical biomarkers and established a risk score model based on these proteins and clinicopathological characteristics to assess GC prognosis.
Descritores: Neoplasias Gástricas/mortalidade
Biomarcadores Tumorais/metabolismo
Proteínas de Neoplasias/metabolismo
-Prognóstico
Neoplasias Gástricas/metabolismo
Neoplasias Gástricas/patologia
Imuno-Histoquímica
Regulação Neoplásica da Expressão Gênica
Análise de Sobrevida
Análise Serial de Tecidos
Estadiamento de Neoplasias
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Idoso
Responsável: CL1.1 - Biblioteca Central



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