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Pesquisa : D12.776.765 [Categoria DeCS]
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Id: biblio-951812
Autor: Santos, Alexandra de Andrade; Silveira, Joaquim Albenísio Gomes da; Bonifacio, Aurenivia; Rodrigues, Artenisa Cerqueira; Figueiredo, Márcia do Vale Barreto.
Título: Antioxidant response of cowpea co-inoculated with plant growth-promoting bacteria under salt stress
Fonte: Braz. j. microbiol;49(3):513-521, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Soil salinity is an important abiotic stress worldwide, and salt-induced oxidative stress can have detrimental effects on the biological nitrogen fixation. We hypothesized that co-inoculation of cowpea plants with Bradyrhizobium and plant growth-promoting bacteria would minimize the deleterious effects of salt stress via the induction of enzymatic and non-enzymatic antioxidative protection. To test our hypothesis, cowpea seeds were inoculated with Bradyrhizobium or co-inoculated with Bradyrhizobium and plant growth-promoting bacteria and then submitted to salt stress. Afterward, the cowpea nodules were collected, and the levels of hydrogen peroxide; lipid peroxidation; total, reduced and oxidized forms of ascorbate and glutathione; and superoxide dismutase, catalase and phenol peroxidase activities were evaluated. The sodium and potassium ion concentrations were measured in shoot samples. Cowpea plants did not present significant differences in sodium and potassium levels when grown under non-saline conditions, but sodium content was strongly increased under salt stress conditions. Under non-saline and salt stress conditions, plants co-inoculated with Bradyrhizobium and Actinomadura or co-inoculated with Bradyrhizobium and Paenibacillus graminis showed lower hydrogen peroxide content in their nodules, whereas lipid peroxidation was increased by 31% in plants that were subjected to salt stress. Furthermore, cowpea nodules co-inoculated with Bradyrhizobium and plant growth-promoting bacteria and exposed to salt stress displayed significant alterations in the total, reduced and oxidized forms of ascorbate and glutathione. Inoculation with Bradyrhizobium and plant growth-promoting bacteria induced increased superoxide dismutase, catalase and phenol peroxidase activities in the nodules of cowpea plants exposed to salt stress. The catalase activity in plants co-inoculated with Bradyrhizobium and Streptomyces was 55% greater than in plants inoculated with Bradyrhizobium alone, and this value was remarkably greater than that in the other treatments. These results reinforce the beneficial effects of plant growth-promoting bacteria on the antioxidant system that detoxifies reactive oxygen species. We concluded that the combination of Bradyrhizobium and plant growth-promoting bacteria induces positive responses for coping with salt-induced oxidative stress in cowpea nodules, mainly in plants co-inoculated with Bradyrhizobium and P. graminis or co-inoculated with Bradyrhizobium and Bacillus.
Descritores: Cloreto de Sódio/metabolismo
Bradyrhizobium/fisiologia
Inoculantes Agrícolas/fisiologia
Vigna/microbiologia
Antioxidantes/metabolismo
-Proteínas de Plantas/metabolismo
Estresse Fisiológico
Superóxido Dismutase/metabolismo
Peroxidação de Lipídeos
Catalase/metabolismo
Peroxidase/metabolismo
Estresse Oxidativo
Salinidade
Vigna/crescimento & desenvolvimento
Vigna/metabolismo
Glutationa/metabolismo
Responsável: BR1.1 - BIREME


  2 / 138 LILACS  
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Id: biblio-839367
Autor: Santos, Silvana Gomes dos; Silva, Paula Renata Alves da; Garcia, Andres Calderin; Zilli, Jerri Édson; Berbara, Ricardo Luis Louro.
Título: Dark septate endophyte decreases stress on rice plants
Fonte: Braz. j. microbiol;48(2):333-341, April.-June 2017. tab, graf.
Idioma: en.
Projeto: Embrapa; . Fundação de Amparo à Pesquisa no Estado do Rio de Janeiro; . National Council for Scientific and Technological Development; . Fundação de Amparo à Pesquisa de Minas Gerais.
Resumo: Abstract Abiotic stress is one of the major limiting factors for plant development and productivity, which makes it important to identify microorganisms capable of increasing plant tolerance to stress. Dark septate endophytes can be symbionts of plants. In the present study, we evaluated the ability of dark septate endophytes isolates to reduce the effects of water stress in the rice varieties Nipponbare and Piauí. The experiments were performed under gnotobiotic conditions, and the water stress was induced with PEG. Four dark septate endophytes were isolated from the roots of wild rice (Oryza glumaepatula) collected from the Brazilian Amazon. Plant height as well as shoot and root fresh and dry matter were measured. Leaf protein concentrations and antioxidant enzyme activity were also estimated. The dark septate endophytes were grown in vitro in Petri dishes containing culture medium; they exhibited different levels of tolerance to salinity and water stress. The two rice varieties tested responded differently to inoculation with dark septate endophytes. Endophytes promoted rice plant growth both in the presence and in the absence of a water deficit. Decreased oxidative stress in plants in response to inoculation was observed in nearly all inoculated treatments, as indicated by the decrease in antioxidant enzyme activity. Dark septate endophytes fungi were shown to increase the tolerance of rice plants to stress caused by water deficiency.
Descritores: Oryza/fisiologia
Oryza/microbiologia
Estresse Fisiológico
Desidratação
Endófitos/crescimento & desenvolvimento
-Proteínas de Plantas/análise
Oryza/enzimologia
Brasil
Raízes de Plantas/microbiologia
Endófitos/isolamento & purificação
Antioxidantes/análise
Responsável: BR1.1 - BIREME


  3 / 138 LILACS  
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Moreira, Renato de Azevedo
Cavada, Benildo Sousa
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Id: lil-623973
Autor: Moreira, Renato de Azevedo; Ainouz, Iracema Lima; Oliveira, José Tadeu Abreu de; Cavada, Benildo Sousa.
Título: Plant lectins, chemical and biological aspects
Fonte: Mem. Inst. Oswaldo Cruz;86(supl.2):211-218, 1991. tab.
Idioma: en.
Conferência: Apresentado em: Brazilian-Sino Symposium on Chemistry and Pharmacology of Natural Products, Rio de Janeiro, Dec. 10-14, 1989.
Resumo: Lectins, carbohydrate-binding proteins of non-immune origin, that agglutinate cells or precipitate polysaccharides and glycoconjugates, are well distributed in nature, mainly in the Plant Kingdom. The great majority of the plante lectins are present in seed cotyledons where they are found in the cytoplasm or int he protein bodies, although they have also been found in roots, stems and leaves. Due to their peculiar properties, the lectins are used as a tool both for analytical and preparative purposes in biochemistry, cellular biology, immunology and related areas. In agriculture and medicine the use of lectins greatly improved in the last few years. The lextins, with few exceptions, are glycoproteins, need divalent cations to display full activity and are, in general, oligomers with variable molecular weight. Although the studies on lectins have completed a century, their role in nature is yet ynknown . Several hypotheses on their physiological functions have been suggested. Thus, lectins could play important roles in defense against pathogens, plant-microorganism symbiosis, cell organization, embryo morphogenesis, phagocytosis, cell wall elongation, pollen recognition and as reserve proteins. A brief review on the general properties and roles of the lectins is given.
Descritores: Proteínas de Plantas/farmacologia
Plantas/química
Lectinas/isolamento & purificação
Lectinas/química
-Proteínas na Dieta/farmacologia
Glicoproteínas/farmacologia
Metabolismo dos Carboidratos
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


  4 / 138 LILACS  
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Cordeiro, R. S. B
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Id: lil-623954
Autor: Castro Faria Neto, H. C; Cordeiro, R. S. B; Martins, M. A; Correia da Silva, A. C. V; Bozza, P. T; Sousa, M. V; Morhy, L.
Título: Enterolobin induces rat paw oedema independently of PAF-acether
Fonte: Mem. Inst. Oswaldo Cruz;86(supl.2):129-131, 1991. ilus, tab.
Idioma: en.
Conferência: Apresentado em: Brazilian-Sino Symposium on Chemistry and Pharmacology of Natural Products, Rio de Janeiro, Dec. 10-14, 1989.
Resumo: The potential participation of PAF-acether (PAF) on the paw oedema triggered by enterolobin was investigated. Intraplantar injections of enterolobin )5-20 µg/paw) yielded a dose response curve for edema which appeared after 30 min, peaked in the interval between 2-4 h and faded after 24h. The pre-treatment with BN 52021, but not with other PAF antagonists such as PCA 4248 or WEB 2086, significantly blocked enterolobin-induced oedema. To clarify better the discrepant results obtained with the PAF antagonists, desensitization to PAF was performed. The oedema triggered by enterolobin was not modified in paf desensitized animals. It was concluded that the paw inflammation induced by enterolobin does not require PAF mechanism.
Descritores: Proteínas de Plantas/antagonistas & inibidores
Proteínas de Plantas/toxicidade
Azepinas/farmacologia
Triazóis/farmacologia
Di-Hidropiridinas/farmacologia
Fator de Ativação de Plaquetas/antagonistas & inibidores
Fator de Ativação de Plaquetas/fisiologia
-Ratos Wistar
Ginkgolídeos
Diterpenos
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


  5 / 138 LILACS  
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Id: lil-623945
Autor: Xavier Filho, J.
Título: The resistance of seeds of cowpea (Vigna unguiculata) to the cowpea weevil (Callosobruchus maculatus)
Fonte: Mem. Inst. Oswaldo Cruz;86(supl.2):75-77, 1991.
Idioma: en.
Conferência: Apresentado em: Brazilian-Sino Symposium on Chemistry and Pharmacology of Natural Products, Rio de Janeiro, Dec. 10-14, 1989.
Resumo: Cowpea (Vigna unguiculata) seeds are heavily damaged during storage by the bruchid Callosobruchus maculatus. Seeds of some Nigerian varieties showed a strong resistance to this bruchid. By utilizing biochemical and entomological techniques we were able to rule out the paticipation of proteolytic enzyme (trypsin, chimotrypsin, subtilisin and papain) inhibitors, lectins, and tannins in the resistance mechanisms. Fractionation of the seed meal of a resistant variety suggests that the factor(s) responsible for the effect is (are) concentrate in the globulin fraction.
Descritores: Proteínas de Plantas/farmacologia
Besouros/efeitos dos fármacos
Produtos Agrícolas/parasitologia
Eletroforese em Gel de Poliacrilamida
Proteínas de Armazenamento de Sementes
-Proteínas de Plantas/isolamento & purificação
Phaseolus/química
Limites: Animais
Responsável: BR1.1 - BIREME


  6 / 138 LILACS  
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Id: biblio-1022130
Autor: Zhou, Shushan; Jiang, Li; Guan, Shuangxue; Gao, Yongxia; Gao, Qinghua; Wang, Guangdong; Duan, Ke.
Título: Expression profiles of five FT-like genes and functional analysis of PhFT-1 in a Phalaenopsis hybrid
Fonte: Electron. j. biotechnol;31:75-83, Jan. 2018. tab, ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Background: Phalaenopsis is an important ornamental flowering plant that belongs to the Orchidaceae family and is cultivated worldwide. Phalaenopsis has a long juvenile phase; therefore, it is important to understand the genetic elements regulating the transition from vegetative phase to reproductive phase. In this study, FLOWERING LOCUS T (FT) homologs in Phalaenopsis were cloned, and their effects on flowering were analyzed. Results: A total of five FT-like genes were identified in Phalaenopsis. Phylogenetic and expression analyses of these five FT-like genes indicated that some of these genes might participate in the regulation of flowering. A novel FT-like gene, PhFT-1, distantly related to previously reported FT genes in Arabidopsis and other dicot crops, was also found to be a positive regulator of flowering as heterologous expression of PhFT-1 in Arabidopsis causes an early flowering phenotype. Conclusions: Five FT homologous genes from Phalaenopsis orchid were identified, and PhFT-1 positively regulates flowering.
Descritores: Proteínas de Plantas/genética
Arabidopsis
Orchidaceae/genética
Flores/genética
-Reação em Cadeia da Polimerase/métodos
Clonagem Molecular
Genes de Plantas/genética
Biologia Computacional
Orchidaceae/crescimento & desenvolvimento
Flores/crescimento & desenvolvimento
Responsável: CL1.1 - Biblioteca Central


  7 / 138 LILACS  
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Id: biblio-1009164
Autor: Perini, Mauro A; Sin, Ignacio N; Martinez, Gustavo Adolfo; Civello, Pedro M.
Título: Measurement of expansin activity and plant cell wall creep by using a commercial texture analyzer
Fonte: Electron. j. biotechnol;26:12-19, Mar. 2017. ilus, graf, tab.
Idioma: en.
Projeto: Agencia Nacional de Promoción Científica y Tecnológica ANPCyT.
Resumo: Background: Expansins play an important role in cell wall metabolism and fruit softening. Determination of expansin activity is a challenging problem since it depends on measuring cell wall properties by using ad hoc extensometers, a fact that has strongly restricted its study. Then, the objective of the work was to adapt a methodology to measure cell wall creep and expansin activity using a commercial texture meter, equipped with miniature tensile grips and an ad hoc cuvette of easy construction. Results: It was possible to measure hypocotyls acid growth and expansin activity in a reliable and reproducible way, using a commercial texture meter, common equipment found in laboratories of food science or postharvest technology. Expansin activity was detected in protein extracts from cucumber hypocotyls, tomato and strawberry fruits, and statistical differences in expansin activity were found in both fruit models at different ripening stages. Conclusions: The possibility of measuring expansin activity following this adapted protocol with a commercial texture meter could contribute to ease and increase the analysis of expansin in different systems, leading to a better understanding of the properties of these proteins under different experimental conditions.
Descritores: Proteínas de Plantas/metabolismo
Lycopersicon esculentum/metabolismo
Cucumis sativus/metabolismo
Fragaria/metabolismo
-Proteínas de Plantas/análise
Parede Celular/metabolismo
Hipocótilo/crescimento & desenvolvimento
Elasticidade
Frutas/metabolismo
Responsável: CL1.1 - Biblioteca Central


  8 / 138 LILACS  
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Id: biblio-1010154
Autor: Yang, Song; Ding, Meng-Meng; Chen, Fang; Xu, Ying.
Título: Proteomic analysis of latex from Jatropha curcas L. stems and comparison of two classic proteomic sample isolation methods: the phenol extraction and the TCA/acetone extraction
Fonte: Electron. j. biotechnol;27:14-24, May. 2017. ilus, graf, tab.
Idioma: en.
Projeto: 12th Five-Year Plan of China.
Resumo: Background: Jatropha curcas is a wide-spreading latex-rich biodiesel plant with high oil content in seeds that have always been under intense studies. However, studies are lacking on the latex component that is considered rich in proteins with potentially important physiological functions and secondary metabolites that are a promising source for new drugs. The proteomic analysis, which would be the first step to study these substances, was hampered by the presence of interfering components. Phenol extraction and Trichloroacetic acid (TCA)/ acetone extraction, two major plant proteomic isolation methods, were used and compared in this study. Results: We identified 459 proteins from the J. curcas latex proteome using the combination of the two extraction techniques. Although more number of latex proteins were identified by the phenol extraction (401 proteins vs. 123 proteins by the TCA/acetone extraction), only 65 proteins were commonly isolated by both methods. Analysis of the biochemical properties revealed that relatively more number of lower isoelectric point (pI) proteins were isolated by the TCA/acetone method (pI mode: 4.79, 6.51 for phenol). Moreover, GO, COG, and KEGG analyses showed that certain classes/categories/pathways annotated more number of proteins than others, and most of them had proportionally comparable protein counts by both the methods, however, with exemplified exceptions. Conclusions: A large number of proteins were found and exclusively identified by either method, indicating that a better proteome coverage of plant samples in a similar context needs the combined use of multiple isolation methods. In addition, the core biological function of the latex may be uncovered by certain GO, COG, and KEGG classes/categories/pathways that annotate more proteins.
Descritores: Proteínas de Plantas/análise
Caules de Planta/química
Jatropha
Proteômica/métodos
Látex/química
-Acetona/química
Proteínas de Plantas/isolamento & purificação
Cromatografia Líquida
Fenol/química
Espectrometria de Massas por Ionização por Electrospray
Eletroforese em Gel de Poliacrilamida
Espectrometria de Massas em Tandem
Tipo de Publ: Estudo Comparativo
Responsável: CL1.1 - Biblioteca Central


  9 / 138 LILACS  
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Id: biblio-889126
Autor: Ansarypour, Zahra; Shahpiri, Azar.
Título: Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance
Fonte: Braz. j. microbiol;48(3):537-543, July-Sept. 2017. graf.
Idioma: en.
Projeto: Iran National Science Foundation.
Resumo: Abstract Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd2+, H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S. cerevisiae. After verification of heterologous expression of OsMTI-1b, the new strain and control were grown under stress conditions. In comparison to control strain, the transformed S. cerevisiae cells expressing OsMTI-1b showed more tolerance to Cd2+ and accumulated more Cd2+ ions when they were grown in the medium containing CdCl2. In addition, the heterologous expression of GST-OsMTI-1b conferred H2O2 and ethanol tolerance to S. cerevisiae cells. The results indicate that heterologous expression of plant MT isoforms can enhance the tolerance of S. cerevisiae to multiple stresses.
Descritores: Proteínas de Plantas/genética
Oryza/genética
Saccharomyces cerevisiae/metabolismo
Cádmio/metabolismo
Expressão Gênica
Etanol/metabolismo
Peróxido de Hidrogênio/metabolismo
Metalotioneína/genética
-Proteínas de Plantas/metabolismo
Saccharomyces cerevisiae/genética
Estresse Oxidativo
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Metalotioneína/metabolismo
Responsável: BR1.1 - BIREME


  10 / 138 LILACS  
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Id: biblio-1021325
Autor: Al-Soqeer, Abdulrahman A; Alsubaie, Qasi D; Motawei, Mohamed I; Mousa, Hassan M; Abdel-Salam, Ahmed M.
Título: Isolation and identification of allergens and biogenic amines of Prosopis juliflora genotypes
Fonte: Electron. j. biotechnol;30:24-32, nov. 2017. tab, ilus, graf.
Idioma: en.
Resumo: Background: Prosopis, or mesquite (Prosopis juliflora (Sw.) DC.), was introduced in Saudi Arabia several decades ago and is heavily used in street, roadside, and park plantations. It shows great adaptation to the prevailing climatic conditions such as high temperature, severe drought, and salinity and spreads naturally in many parts of the Kingdom. This research was conducted to isolate allergen proteins and biogenic amines from the pollen grains of P. juliflora genotypes in Saudi Arabia from two regions, namely Al-Qassim and Eastern regions. Results: The results showed that 18 different allergen proteins were detected in P. juliflora genotypes, with molecular weight ranging from 14 to 97 kDa. Moreover, P. juliflora genotypes from the two studied regions contained eight biogenic amines, namely histamine, tyramine, tryptamine, ß-phenylethylamine, butricine, codapherine, spermidine, and spermine. All genotypes from the Al-Qassim region were found to contain all eight amines, while in the Eastern region, histamine was absent in three genotypes, spermine was absent in six genotypes, and spermidine was absent in three genotypes. Genotypes B23, E20, and E21 had the lowest biogenic amine quantity. Conclusions: All identified proteins from mesquite trees from both regions (Eastern and Al-Qassim) cause allergies in patients who are sensitive to pollen grains. Bioamines, except histamine and tyramine, were recorded at varying concentrations in different genotypes.
Descritores: Pólen/química
Aminas Biogênicas/isolamento & purificação
Alérgenos/isolamento & purificação
Prosopis
-Proteínas de Plantas/isolamento & purificação
Histamina/isolamento & purificação
Tiramina/isolamento & purificação
Cromatografia Líquida de Alta Pressão
Eletroforese em Gel de Poliacrilamida
Genótipo
Peso Molecular
Responsável: CL1.1 - Biblioteca Central



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