Base de dados : LILACS
Pesquisa : D12.776.828.868.955 [Categoria DeCS]
Referências encontradas : 2 [refinar]
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Id: biblio-1041442
Autor: Queiroz, Sabrina Ribeiro de Almeida; Silva Júnior, José Valter Joaquim; Silva, Andréa Nazaré Monteiro Rangel da; Carvalho, Amanda Gomes de Oliveira; Santos, Jefferson José da Silva; Gil, Laura Helena Vega Gonzales.
Título: Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Fonte: Rev. Soc. Bras. Med. Trop;51(1):66-70, Jan.-Feb. 2018. graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. METHODS: HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs. RESULTS: The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs. CONCLUSIONS: HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
Descritores: Replicação Viral/imunologia
Vírus da Febre Amarela/imunologia
Montagem de Vírus/imunologia
Vacinas de Partículas Semelhantes a Vírus/imunologia
-Replicação Viral/genética
Vírus da Febre Amarela/genética
Montagem de Vírus/genética
Técnica Indireta de Fluorescência para Anticorpo
Proteínas de Fluorescência Verde
Células HEK293
Vacinas de Partículas Semelhantes a Vírus/genética
Citometria de Fluxo
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-703041
Autor: Wei, Lanlan; Chu, Ming; Zhang, Qingmeng; Wang, Yan; Shang, Qinglong; Zhang, Yunyan; Zhang, Guangmei.
Título: Interleukin-12 gene adjuvant increases the immunogenicity of virus-like particles of human papillomavirus type 16 regional variant strain
Fonte: Braz. j. infect. dis;18(1):65-70, Jan-Feb/2014. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Natural Science Foundation of China; . National Natural Science Foundation of China; . Natural Science Foundation of Heilongjiang Province; . Scientific Research Foundation for ROCS, SEM; . National Key Basic Research Program of MOST.
Resumo: Objectives: To analyze the immunogenicity of virus-like particles (VLP) of human papillomavirus type 16 (HPV16) isolated in East China and the adjuvant potential of interleukin-12 (IL-12). Methods: The variant HPV16 L1VLP expressed in sf9 insect cells were purified with cesium chloride gradient centrifugation. BALB/c mice were vaccinated with VLP (L1N), VLP with Freund's adjuvant (L1A) or VLP with IL-12 recombinant plasmid (L1P). HPV16 VLP specific IgG and IFN-γ level in the serum were detected by ELISA, and the percentage of CD4+ and CD8+ in spleen cells was detected with flow cytometry. Results: The titers of serum IgG antibodies in vaccinated groups were higher than in negative control and the serum antibodies mainly recognized conformation-dependent HPV16 VLP epitopes. Splenic CD4+ and CD8+ T cell subsets increased after vaccination in every experimental group, and CD8+ increased obviously in L1P group. The ratio of CD4+/CD8+ decreased in L1P group and increased in the other two groups, compared to control group. Vaccination induced specific secretion of IFN-γ in the serum of vaccinated group (p < 0.05), especially in the L1P group. Conclusions: VLP of HPV16 variant strain isolated in East China could induce humoral immunity and cellular immunity in mice, and IL-12 recombinant plasmid can enhance cellular immunity. .
Descritores: /imunologia
HUMAN PAPILLOMAVIRUS ABNORMALITIES, MULTIPLE/imunologia
/sangue
INTERLEUKIN-ABETALIPOPROTEINEMIA/sangue
/genética
INTERLEUKIN-ABETALIPOPROTEINEMIA/genética
Infecções por Papillomavirus/prevenção & controle
Vacinas de Partículas Semelhantes a Vírus/imunologia
-Adjuvantes Imunológicos
Anticorpos Antivirais/sangue
Anticorpos Antivirais/imunologia
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Imunidade Celular/imunologia
Imunoglobulina G/sangue
Imunoglobulina G/imunologia
/imunologia
INTERLEUKIN-ABETALIPOPROTEINEMIA/imunologia
Camundongos Endogâmicos BALB C
Dados de Sequência Molecular
Infecções por Papillomavirus/imunologia
Limites: Animais
Feminino
Humanos
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



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