||Wei, Lanlan; Chu, Ming; Zhang, Qingmeng; Wang, Yan; Shang, Qinglong; Zhang, Yunyan; Zhang, Guangmei.|
||Interleukin-12 gene adjuvant increases the immunogenicity of virus-like particles of human papillomavirus type 16 regional variant strain|
||Braz. j. infect. dis;18(1):65-70, Jan-Feb/2014. tab, graf.
||National Natural Science Foundation of China; . National Natural Science Foundation of China; . National Natural Science Foundation of China; . Natural Science Foundation of Heilongjiang Province; . Scientific Research Foundation for ROCS, SEM; . National Key Basic Research Program of MOST.
||Objectives: To analyze the immunogenicity of virus-like particles (VLP) of human papillomavirus type 16 (HPV16) isolated in East China and the adjuvant potential of interleukin-12 (IL-12). Methods: The variant HPV16 L1VLP expressed in sf9 insect cells were purified with cesium chloride gradient centrifugation. BALB/c mice were vaccinated with VLP (L1N), VLP with Freund's adjuvant (L1A) or VLP with IL-12 recombinant plasmid (L1P). HPV16 VLP specific IgG and IFN-γ level in the serum were detected by ELISA, and the percentage of CD4+ and CD8+ in spleen cells was detected with flow cytometry. Results: The titers of serum IgG antibodies in vaccinated groups were higher than in negative control and the serum antibodies mainly recognized conformation-dependent HPV16 VLP epitopes. Splenic CD4+ and CD8+ T cell subsets increased after vaccination in every experimental group, and CD8+ increased obviously in L1P group. The ratio of CD4+/CD8+ decreased in L1P group and increased in the other two groups, compared to control group. Vaccination induced specific secretion of IFN-γ in the serum of vaccinated group (p < 0.05), especially in the L1P group. Conclusions: VLP of HPV16 variant strain isolated in East China could induce humoral immunity and cellular immunity in mice, and IL-12 recombinant plasmid can enhance cellular immunity. .|
HUMAN PAPILLOMAVIRUS ABNORMALITIES, MULTIPLE/imunologia
Infecções por Papillomavirus/prevenção & controle
Vacinas de Partículas Semelhantes a Vírus/imunologia
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Camundongos Endogâmicos BALB C
Dados de Sequência Molecular
Infecções por Papillomavirus/imunologia
| Tipo de Publ:
||Research Support, Non-U.S. Gov't|
||BR1.1 - BIREME|