Base de dados : LILACS
Pesquisa : D12.776.828.868.955 [Categoria DeCS]
Referências encontradas : 1 [refinar]
Mostrando: 1 .. 1   no formato [Detalhado]

página 1 de 1

  1 / 1 LILACS  
para imprimir
Texto completo SciELO Brasil
Texto completo
Id: lil-703041
Autor: Wei, Lanlan; Chu, Ming; Zhang, Qingmeng; Wang, Yan; Shang, Qinglong; Zhang, Yunyan; Zhang, Guangmei.
Título: Interleukin-12 gene adjuvant increases the immunogenicity of virus-like particles of human papillomavirus type 16 regional variant strain
Fonte: Braz. j. infect. dis;18(1):65-70, Jan-Feb/2014. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Natural Science Foundation of China; . National Natural Science Foundation of China; . Natural Science Foundation of Heilongjiang Province; . Scientific Research Foundation for ROCS, SEM; . National Key Basic Research Program of MOST.
Resumo: Objectives: To analyze the immunogenicity of virus-like particles (VLP) of human papillomavirus type 16 (HPV16) isolated in East China and the adjuvant potential of interleukin-12 (IL-12). Methods: The variant HPV16 L1VLP expressed in sf9 insect cells were purified with cesium chloride gradient centrifugation. BALB/c mice were vaccinated with VLP (L1N), VLP with Freund's adjuvant (L1A) or VLP with IL-12 recombinant plasmid (L1P). HPV16 VLP specific IgG and IFN-γ level in the serum were detected by ELISA, and the percentage of CD4+ and CD8+ in spleen cells was detected with flow cytometry. Results: The titers of serum IgG antibodies in vaccinated groups were higher than in negative control and the serum antibodies mainly recognized conformation-dependent HPV16 VLP epitopes. Splenic CD4+ and CD8+ T cell subsets increased after vaccination in every experimental group, and CD8+ increased obviously in L1P group. The ratio of CD4+/CD8+ decreased in L1P group and increased in the other two groups, compared to control group. Vaccination induced specific secretion of IFN-γ in the serum of vaccinated group (p < 0.05), especially in the L1P group. Conclusions: VLP of HPV16 variant strain isolated in East China could induce humoral immunity and cellular immunity in mice, and IL-12 recombinant plasmid can enhance cellular immunity. .
Descritores: /imunologia
Infecções por Papillomavirus/prevenção & controle
Vacinas de Partículas Semelhantes a Vírus/imunologia
-Adjuvantes Imunológicos
Anticorpos Antivirais/sangue
Anticorpos Antivirais/imunologia
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Imunidade Celular/imunologia
Imunoglobulina G/sangue
Imunoglobulina G/imunologia
Camundongos Endogâmicos BALB C
Dados de Sequência Molecular
Infecções por Papillomavirus/imunologia
Limites: Animais
Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME

página 1 de 1

Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde