Base de dados : LILACS
Pesquisa : D12.776.835.725.868.124 [Categoria DeCS]
Referências encontradas : 1 [refinar]
Mostrando: 1 .. 1   no formato [Detalhado]

página 1 de 1

  1 / 1 LILACS  
             
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-600693
Autor: Goulart-Silva, F; Serrano-Nascimento, C; Nunes, M. T.
Título: Hypothyroidism decreases proinsulin gene expression and the attachment of its mRNA and eEF1A protein to the actin cytoskeleton of INS-1E cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;44(10):1060-1067, Oct. 2011. ilus, tab.
Idioma: en.
Resumo: The actions of thyroid hormone (TH) on pancreatic beta cells have not been thoroughly explored, with current knowledge being limited to the modulation of insulin secretion in response to glucose, and beta cell viability by regulation of pro-mitotic and pro-apoptotic factors. Therefore, the effects of TH on proinsulin gene expression are not known. This led us to measure: a) proinsulin mRNA expression, b) proinsulin transcripts and eEF1A protein binding to the actin cytoskeleton, c) actin cytoskeleton arrangement, and d) proinsulin mRNA poly(A) tail length modulation in INS-1E cells cultured in different media containing: i) normal fetal bovine serum - FBS (control); ii) normal FBS plus 1 µM or 10 nM T3, for 12 h, and iii) FBS depleted of TH for 24 h (Tx). A decrease in proinsulin mRNA content and attachment to the cytoskeleton were observed in hypothyroid (Tx) beta cells. The amount of eEF1A protein anchored to the cytoskeleton was also reduced in hypothyroidism, and it is worth mentioning that eEF1A is essential to attach transcripts to the cytoskeleton, which might modulate their stability and rate of translation. Proinsulin poly(A) tail length and cytoskeleton arrangement remained unchanged in hypothyroidism. T3 treatment of control cells for 12 h did not induce any changes in the parameters studied. The data indicate that TH is important for proinsulin mRNA expression and translation, since its total amount and attachment to the cytoskeleton are decreased in hypothyroid beta cells, providing evidence that effects of TH on carbohydrate metabolism also include the control of proinsulin gene expression.
Descritores: Citoesqueleto de Actina/metabolismo
Fator de Iniciação 1 em Eucariotos/metabolismo
Hipotireoidismo/metabolismo
Células Secretoras de Insulina/metabolismo
Proinsulina/genética
RNA Mensageiro/metabolismo
-Expressão Gênica
Hipotireoidismo/genética
Proinsulina/biossíntese
RNA Mensageiro/genética
Limites: Animais
Bovinos
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME



página 1 de 1
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde