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Id: biblio-1047373
Autor: Shi, Xiaodong; Wu, Yan; Dai, Tingwei; Gu, Yuxi; Wang, Linghui; Qin, Xiaobo; Xu, Ying; Chen, Fang.
Título: JcZFP8, a C2H2 zinc finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco
Fonte: Electron. j. biotechnol;34:76-82, july. 2018. ilus, graf.
Idioma: en.
Projeto: National Key Technology R&D Program of 12th Five-Year Plan of China; . Open Foundation of Key Laboratory of Molecular Genetics, China National Tobacco Corporation (Guizhou Academy of Tobacco Science); . Special Project for Breeding and Cultivation of GMO Varieties of Ministry of Agriculture.
Resumo: Background: Jatropha curcas L., as an important strategic biofuel resource with considerable economic potential, has attracted worldwide attention. However, J. curcas has yet to be domesticated. Plant height, an important agronomic trait of J. curcas, has not been sufficiently improved, and the genetic regulation of this trait in J. curcas is not fully understood. Zinc finger proteins (ZFPs), a class of transcription factors, have previously been shown to play critical roles in regulating multiple aspects of plant growth and development and may accordingly be implicated in the genetic regulation of plant height in J. curcas. Results: In this study, we cloned JcZFP8, a C2H2 ZFP gene in J. curcas. We found that the JcZFP8 protein was localized in the nucleus and contained a conserved QALGGH motif in its C2H2 structure. Furthermore, ectopic expression of JcZFP8 under the control of the 35S promoter in transgenic tobacco resulted in dwarf plants with malformed leaves. However, when JcZFP8 was knocked out, the transgenic tobacco did not show the dwarf phenotype. After treatment with the gibberellic acid (GA) biosynthesis inhibitor paclobutrazol (PAC), the dwarf phenotype was more severe than plants that did not receive the PAC treatment, whereas application of exogenous gibberellin3 (GA3) reduced the dwarf phenotype in transgenic plants. Conclusions: The results of this study indicate that JcZFP8 may play a role in J. curcas plant phenotype through GA-related pathways. Our findings may help us to understand the genetic regulation of plant development in J. curcas and to accelerate breeding progress through engineering of the GA metabolic pathway in this plant. How to cite: Shi X,Wu Y, Dai T, et al. JcZFP8, a C2H2 zinc-finger protein gene from Jatropha curcas, influences plant development in transgenic tobacco.
Descritores: Tabaco/genética
Jatropha
Desenvolvimento Vegetal
Dedos de Zinco CYS2-HIS2/genética
-Reguladores de Crescimento de Plantas/genética
Fatores de Transcrição
Triazóis
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Clonagem Molecular
Regulação da Expressão Gênica de Plantas
Reação em Cadeia da Polimerase em Tempo Real
Giberelinas
Responsável: CL1.1 - Biblioteca Central


  2 / 206 LILACS  
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Id: biblio-1283167
Autor: Alsagaby, Suliman A; Brewis, Ian A; Vijayakumar, Rajendran; Alhumaydhi, Fahad A; Alwashmi, Ameen S; Alharbi, Naif K; Al Abdulmonem, Waleed; Premanathan, Mariappan; Pratti, Guy; Fegan, Christopher; Pepper, Christopher; Brennan, Paul.
Título: Proteomics-based identification of cancer-associated proteins in chronic lymphocytic leukaemia
Fonte: Electron. j. biotechnol;52:1-12, July. 2021. tab, ilus, graf.
Idioma: en.
Resumo: BACKGROUND: Chronic lymphocytic leukaemia (CLL) is a neoplasm of B-cells characterized by variable prognosis. Exploring the proteome of CLL cells may provide insights into the disease. Therefore, eleven proteomics experiments were conducted on eleven primary CLL samples. RESULTS: We reported a CLL proteome consisting of 919 proteins (false discovery rate (FDR) 1%) whose identification was based on the sequencing of two or more distinct peptides (FDR of peptide sequencing 1%). Mass spectrometry-based protein identification was validated for four different proteins using Western blotting and specific antibodies in different CLL samples. Small sizes of nucleolin (~57 kDa and ~68 kDa) showed a potential association with good prognosis CLL cells (n = 8, p < 0.01). Compared with normal B-cells, CLL cells over-expressed thyroid hormone receptor-associated protein 3 (THRAP3; n = 9; p = 0.00007), which is implicated in cell proliferation; and heterochromatin protein 1-binding protein 3 (HP1BP3; n = 10; p = 0.0002), which promotes cell survival and tumourogenesis. A smaller form of HP1BP3, which may correspond to HP1BP3 isoform-2, was specifically identified in normal B-cells (n = 10; p = 0.0001). HP1BP3 and THRAP3 predicted poor prognosis of CLL (p 0.05). Consistently, THRAP3 and HP1BP3 were found to be associated with cancer-related pathways (p 0.05). CONCLUSIONS: Our findings add to the known proteome of CLL and confirm the prognostic importance of two novel cancer-associated proteins in this disease.
Descritores: Leucemia Linfocítica Crônica de Células B
Biomarcadores Tumorais/análise
-Espectrometria de Massas
Fatores de Transcrição/análise
Proteínas Nucleares/análise
Western Blotting
Cromatografia Líquida
Proteômica
Proteínas de Ligação a DNA/análise
Responsável: CL1.1 - Biblioteca Central


  3 / 206 LILACS  
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Id: biblio-1253006
Autor: Wang, Meng-jie; Abbas Raza, Sayed Haidar; Wu, Qiang; Xue, Cai-hua; Liu, Jia-hua; Zhang, Long-fei; Zhang, Wen-ying; Wang, Ai-chao; Wu, Hua.
Título: Cichoric acid from extracted Echinacea purpurea induces the proliferation and apoptosis of peripheral blood mononuclear cells from yaks
Fonte: Electron. j. biotechnol;47:17-28, sept. 2020. ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Applied Basic Research of Foundation of Qinghai Province.
Resumo: BACKGROUND: Cichoric acid (CA) is extracted from Echinacea purpurea. It is well known and widely used for its immunological function. However, the effect of CA on peripheral blood mononuclear cells (PBMCs) from yaks is still unclear. This study investigated the potential influences of CA on the proliferation, cytokine induction, and apoptosis of PBMCs from Datong yak in vivo, and aimed to provide a basis for exploring the pharmacological activities of CA on yaks. RESULTS: In this study, CA promoted PBMCs proliferation by combining concanavalin A (Con A) and exhibited a dose-dependent effect as demonstrated by a Cell Counting Kit-8. The concentration of 60 µg/ml CA was the best and promoted the transformation from the G0/G1 phase to the S and G2/M phases with Con A. Furthermore, 60 µg/ml CA significantly increased IL-2, IL-6, and IFN-γ levels and PCNA, CDK4 and Bcl-2 expression levels, but it significantly inhibited the TP53, Bax, and Caspase-3 expression levels. Transcriptome analysis revealed a total of 6807 differentially expressed genes (DEGs) between the CA treatment and control groups. Of these genes, 3788 were significantly upregulated and 3019 were downregulated. Gene Ontology and pathway analysis revealed that DEGs were enriched in cell proliferation and immune function signaling pathways. The expression level of some transcription factors (BTB, Ras, RRM_1, and zf-C2H2) and genes (CCNF, CCND1, and CDK4) related to PBMCs proliferation in yaks were significantly promoted after CA treatment. By contrast, anti-proliferation-associated genes (TP53 and CDKN1A) were inhibited. CONCLUSIONS: In summary, CA could regulate the immune function of yaks by promoting proliferation and inhibiting inflammation and apoptosis of PBMCs.
Descritores: Succinatos/farmacologia
Ácidos Cafeicos/farmacologia
Leucócitos Mononucleares/efeitos dos fármacos
Echinacea/química
Proliferação de Células/efeitos dos fármacos
-Fatores de Transcrição
Ensaio de Imunoadsorção Enzimática
Leucócitos Mononucleares/citologia
Western Blotting
Citocinas
Apoptose/efeitos dos fármacos
Concanavalina A/farmacologia
Reação em Cadeia da Polimerase em Tempo Real
RNA-Seq
Limites: Animais
Bovinos
Responsável: CL1.1 - Biblioteca Central


  4 / 206 LILACS  
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Id: biblio-1177447
Autor: Bai, Yu; Li, Wenliang; Xu, Guangyu; Cui, Guihua.
Título: A bioinformatics approach revealed the transcription factors of Helicobacter pylori pathogenic genes and their regulatory network nodes
Fonte: Electron. j. biotechnol;45:53-59, May 15, 2020. tab, ilus.
Idioma: en.
Projeto: Department of Education of Jilin Province; . Jilin Province Traditional Chinese Medicine Science and Technology Project; . Jilin Province Health and Family Planning Commission; . Jilin Science and Technology Innovation Development Plan Project.
Resumo: BACKGROUND: Helicobacter pylori is a chronic pathogenic bacteria that causes gastric mucosal damage through various host-related and pathogen-related factors. Thus, a single gene research cannot fully explain its pathogenicity. PURPOSE OF STUDY: It is necessary to establish a Helicobacter pylori pathogenic gene transcription factor regulatory network (TFRN) and study its central nodes. RESULTS: The expression data of Helicobacter pylori pathogenic genes were obtained through GEO Datasets of NCBI. The genes were screened using linear model-empirical Bayesian statistics in R language Limma package combined with the conventional t-test; the results identified 1231 differentially expressed genes. The functional analysis (gene ontology-analysis) and signal pathway analysis (pathway-analysis) of differentially expressed genes were performed using the DAVID and KEGG databases, respectively. The pathogenic gene regulatory network was constructed by integrating transcriptional regulatory element database (TRED); the disease-related analysis of the pathogenic genes was conducted using the DAVID annotation tool. Five pathogenic genes (Nos2, Il5, Colla1, Tnf, and Nfkb1) and their transcription factors (Jun, Cebpa, Egrl, Ppara, and Il6) were found to suppress the host immune function and enhance the pathogenicity of Helicobacter pylori by regulating the host immune system. CONCLUSIONS: This effect was largely mediated via three signaling pathways: Tnf pathway, PI3K Akt pathway, and Jak­STAT pathway. The pathogenicity of Helicobacter pylori is closely related to the body's immune and inflammatory system. A better understanding of the correlation of the pathogenic factors with the host immune and inflammatory factors may help to determine the precise pathogenic mechanism of H. pylori infection.
Descritores: Helicobacter pylori/genética
Helicobacter pylori/patogenicidade
Biologia Computacional
-Fatores de Transcrição
Citocinas
Fatores de Virulência
Gastrite/imunologia
Gastrite/microbiologia
Genes Bacterianos
Sistema Imunitário
Inflamação
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-974907
Autor: Kong, Jiangying; Liu, Zhuo; Cai, Feng; Xu, Xiaocheng; LiuI, Jun.
Título: Relationship between the Asp1104His polymorphism of the nucleotide excision repair gene ERCC5 and treatment sensitivity to oxaliplatin in patients with advanced colorectal cancer in China
Fonte: Clinics;73:e455, 2018. tab, graf.
Idioma: en.
Projeto: Hangzhou Municipal Science and Technology Commission; . Xiaoshan Science and Technology Commission of Hangzhou City.
Resumo: OBJECTIVES: To study the relationship between the Asp1104His polymorphism of the nucleotide excision repair gene ERCC5 and treatment sensitivity to oxaliplatin in patients with advanced colorectal cancer (CRC) in China. METHODS: A group of 226 patients in the Department of Gastrointestinal Oncology at Zhejiang Xiaoshan Hospital from July 2011∼December 2016 and a control group of 226 normal healthy individuals were involved in this study. All patients were first diagnosed with advanced CRC and were treated with oxaliplatin-based chemotherapy. The genotype of ERCC5 at the site of amino acid 1104 was determined by a TaqMan probe-based real-time PCR approach. RESULTS: There were no differences in age or gender between the groups, but the percentages of smokers and individuals with a family history of cancer were significantly higher in the patient group than in the control group. Analysis of the G/C polymorphism frequency among the patients and the healthy controls showed that the frequencies of the CC genotype and the CC+GC genotype were significantly related to CRC, but no significant difference in these frequencies was found between genders. The analysis of the relationship between the 5-year survival rate and different genotypes showed that in the total patient group, regardless of gender, the 5-year survival rate was significantly associated with the Asp1104His polymorphism of ERCC5. CONCLUSIONS: The Asp1104His polymorphism of ERCC5 was associated with the risk and 5-year survival rate of CRC as well as treatment sensitivity to oxaliplatin.
Descritores: Proteínas Nucleares/genética
Neoplasias Colorretais/genética
Neoplasias Colorretais/tratamento farmacológico
Proteínas de Ligação a DNA/genética
Endonucleases/genética
Antineoplásicos/uso terapêutico
-Fatores de Transcrição/genética
Neoplasias Colorretais/mortalidade
Estudos de Casos e Controles
Polimorfismo de Nucleotídeo Único/genética
Estimativa de Kaplan-Meier
Oxaliplatina/uso terapêutico
Genótipo
Estadiamento de Neoplasias
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


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Texto completo SciELO Brasil
Texto completo
Id: biblio-974952
Autor: Ribeiro, Aline Lopes; Caodaglio, Amanda Schiersner; Sichero, Laura.
Título: Regulation of HPV transcription
Fonte: Clinics;73(supl.1):e486s, 2018. graf.
Idioma: en.
Resumo: Human papillomavirus infection is associated with the development of malignant and benign neoplasms. Approximately 40 viral types can infect the anogenital mucosa and are categorized into high- and low-risk oncogenic human papillomavirus, depending on their association with the development of cervical carcinoma. High-risk human papillomavirus 16 and 18 are detected in 55% and 15% of all invasive cervical squamous cell carcinomas worldwide, respectively. Low-risk human papillomavirus 6 and 11 are responsible for 90% of genital warts and are also associated with the development of recurrent respiratory papillomatosis. Human papillomavirus preferentially infects mitotic active cells of the basal layer from both mucosal and cutaneous epithelium through microabrasions. The viral life cycle synchronizes with the epithelial differentiation program, which may be due, in part, to the binding of differentially expressed cellular transcription factors to the long control region throughout the various epithelial layers. This review aimed to summarize the current knowledge regarding the mechanisms by which viral gene expression is regulated and the influence of human papillomavirus heterogeneity upon this phenomenon. A better understanding of the regulatory mechanisms may elucidate the particularities of human papillomavirus-associated pathogenesis and may provide new tools for antiviral therapy.
Descritores: Papillomaviridae/genética
Fatores de Transcrição/genética
Regulação Viral da Expressão Gênica
Infecções por Papillomavirus/virologia
-Papillomaviridae/fisiologia
Proteínas Oncogênicas Virais/genética
Regiões Promotoras Genéticas/genética
Limites: Humanos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  7 / 206 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: biblio-1124208
Autor: Li, Huawei; Guan, Haiying; Zhuo, Qicui; Wang, Zongshuai; Li, Shengdong; Si, Jisheng; Zhang, Bin; Feng, Bo; Kong, Ling-an; Wang, Fahong; Wang, Zheng; Zhang, Lishun.
Título: Genome-wide characterization of the abscisic acid-, stress- and ripening-induced (ASR) gene family in wheat (Triticum aestivum L)
Fonte: Biol. Res;53:23, 2020. tab, graf.
Idioma: en.
Projeto: National Key Research and Development Program of China; . National Natural Science Foundation of China; . Taishan Industry.
Resumo: BACKGROUND: Abscisic acid-, stress-, and ripening-induced (ASR) genes are a class of plant specific transcription factors (TFs), which play important roles in plant development, growth and abiotic stress responses. The wheat ASRs have not been described in genome-wide yet. METHODS: We predicted the transmembrane regions and subcellular localization using the TMHMM server, and Plant-mPLoc server and CELLO v2.5, respectively. Then the phylogeny tree was built by MEGA7. The exon-intron structures, conserved motifs and TFs binding sites were analyzed by GSDS, MEME program and PlantRegMap, respectively. RESULTS: In wheat, 33ASR genes were identified through a genome-wide survey and classified into six groups. Phylogenetic analyses revealed that the TaASR proteins in the same group tightly clustered together, compared with those from other species. Duplication analysis indicated that the TaASR gene family has expanded mainly through tandem and segmental duplication events. Similar gene structures and conserved protein motifs of TaASRs in wheat were identified in the same groups. ASR genes contained various TF binding cites associated with the stress responses in the promoter region. Gene expression was generally associated with the expected group-specific expression pattern in five tissues, including grain, leaf, root, spike and stem, indicating the broad conservation of ASR genes function during wheat evolution. The qRT-PCR analysis revealed that several ASRs were up-regulated in response to NaCl and PEG stress. CONCLUSION: We identified ASR genes in wheat and found that gene duplication events are the main driving force for ASR gene evolution in wheat. The expression of wheat ASR genes was modulated in responses to multiple abiotic stresses, including drought/osmotic and salt stress. The results provided important information for further identifications of the functions of wheat ASR genes and candidate genes for high abiotic stress tolerant wheat breeding.
Descritores: Estresse Fisiológico/genética
Triticum/genética
Ácido Abscísico/análise
Genoma de Planta/genética
Evolução Molecular
Secas
-Filogenia
Fatores de Transcrição/genética
Triticum/classificação
Regulação da Expressão Gênica de Plantas
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
Texto completo
Id: biblio-1089075
Autor: Zhu, Qing; Li, Jingchao; Wu, Qi; Cheng, Yongxia; Zheng, Huizhe; Zhan, Tao; Wang, Hongwei; Yang, Yue; Wang, Hongyan; Liu, Ye; Guo, Sufen.
Título: Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor microenvironment
Fonte: Biol. Res;53:05, 2020. tab, graf.
Idioma: en.
Projeto: Nature Science Foundation of Heilongjiang Province; . National Nature Science Foundation of China.
Resumo: BACKGROUND: LincRNAs have been revealed to be tightly associated with various tumorigeneses and cancer development, but the roles of specific lincRNA on tumor-related angiogenesis was hardly studied. Here, we aimed to investigate whether linc-OIP5 in breast cancer cells affects the angiogenesis of HUVECs and whether the linc-OIP5 regulations are involved in angiogenesis-related Notch and Hippo signaling pathways. METHODS: A trans-well system co-cultured HUVECs with linc-OIP5 knockdown breast cancer cell MDA-MB-231 was utilized to study the proliferation, migration and tube formation abilities of HUVECs and alterations of related signaling indicators in breast cancer cells and their conditioned medium through a series of cell and molecular experiments. RESULTS: Overexpressed linc-OIP5, YAP1, and JAG1 were found in breast cancer cell lines MCF7 and MDA-MB-231 and the expression levels of YAP1 and JAG1 were proportional to the breast cancer tissue grades. MDA-MB-231 cells with linc-OIP5 knockdown led to weakened proliferation, migration, and tube formation capacity of co-cultured HUVECs. Besides, linc-OIP5 knockdown in co-cultured MDA-MB-231 cells showed downregulated YAP1 and JAG1 expression, combined with a reduced JAG1 level in conditioned medium. Furthermore, a disrupted DLL4/Notch/NRP1 signaling in co-cultured HUVECs were also discovered under this condition. CONCLUSION: Hence, linc-OIP5 in MDA-MB-231 breast cancer cells may act on the upstream of the YAP1/Notch/NRP1 signaling circuit to affect proliferation, migration, and tube formation of co-cultured HUVECs in a non-cellular direct contact way through JAG1 in conditioned medium. These findings at least partially provide a new angiogenic signaling circuit in breast cancers and suggest linc-OIP5 could be considered as a therapeutic target in angiogenesis of breast cancers.
Descritores: Fatores de Transcrição/metabolismo
Neoplasias da Mama/patologia
Neuropilina-1/metabolismo
Proteínas Adaptadoras de Transdução de Sinal/metabolismo
Receptores Notch/metabolismo
Microambiente Tumoral
Células Endoteliais da Veia Umbilical Humana/citologia
-Neoplasias da Mama/metabolismo
Imuno-Histoquímica
Transdução de Sinais
Western Blotting
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Linhagem Celular Tumoral
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Feminino
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: biblio-1013789
Autor: Ulloa, María Teresa; Sanhueza, Camila; Henríquez, Tania; Aguayo, Benjamín; Hermosilla, Germán; Porte, Lorena; Dabanch, Jeannette; Braun, Stephanie; Fica, Alberto; Briceño, Isabel; Osorio, Carlos Gonzalo.
Título: Cepas chilenas de origen clínico de Vibrio cholerae no-O1, no-O139 portan los genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF del sistema de secreción T3SS2 presentes en una isla de patogenicidad / Chilean strains of clinical origin of non-O1, non-O139 Vibrio cholerae carry the genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF from secretion system T3SS2 present in an island of pathogenicity
Fonte: Rev. chil. infectol;36(3):312-317, jun. 2019. tab, graf.
Idioma: es.
Resumo: Resumen Introducción. Los factores de virulencia de las cepas de Vibrio cholerae no-O1, no-O139 no son claramente conocidos. La cepa de origen septicémico NN1 Vibrio cholerae no-O1, no-O139 fue secuenciada previamente mediante la plataforma Illumina, detectándose en su genoma un fragmento de la isla de patogenicidad VPaI-7 de V. parahaemolyticus. Objetivo: detectar los genes de virulencia vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF en cepas chilenas clínicas de V. cholerae no-O1, no-O139. Material y Métodos: Un total de 9 cepas chilenas de origen clínico de Vibrio cholerae no-O1, no-O139 aisladas entre 2006-2012 fueron analizadas mediante ensayos de reacción de polimerasa en cadena (RPC, en inglés PCR) convencional para los genes de secreción tipo III codificados en dicha isla: vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF. Adicionalmente se determinó la presencia de los genes de virulencia hylA y rtxA. Además, se realizaron ensayos de repetitive element palindromic PCR (REP-PCR) y Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Resultados: la mayoría (6/9) de las cepas chilenas de V. cholerae no-O1, no-O139 contiene todos los genes de secreción tipo III vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF, codificados en una isla de patogenicidad. Además, el total de las cepas (9/9) contiene los genes de virulencia hylA y rtxA. Conclusión: Estos resultados sugieren fuertemente la posibilidad que dichas cepas posean un potencial de virulencia importante en seres humanos.

Backgound: The virulence factors of the Vibrio cholerae non-O1, non-O139 strains are not clearly known. The strain of septicemic origin NN1 Vibrio cholerae non-O1, non-O139 was sequenced previously by the Illumina platform. A fragment of the pathogenicity island VPaI-7 of V. parahaemolyticus was detected in its genome. Aim: To detect the virulence genes vcsN2, vcsC2, vcsV2, vspD, toxR2 y vopF in Chilean strains of V. cholerae non-O1, non-O139. Methods: A total of 9 Chilean strains of clinical origin of Vibrio cholerae non-O1, non-O139 isolated between 2006-2012 were analyzed by conventional PCR assays for type III secretion genes encoded on that island: vcsN2, vcsC2, vcsV2, vspD, toxR2 and vopF. Additionally, the presence of the virulence genes hylA and rtxA was determined. In addition, REP-PCR and ERIC-PCR assays were performed. Results: most (6/9) Chilean V. cholerae non-O1, non-O139 strains contain the type III secretion genes vcsN2, vcsC2, vcsV2, vspD, toxR2 and vopF, encoded in an island of pathogenicity. In addition, all (9/9) the strains contain the virulence genes hylA and rtxA. Conclusion: These results strongly suggest the possibility that those strains possess an important virulence potential in humans.
Descritores: Proteínas de Bactérias/genética
Fatores de Transcrição/genética
Vibrio cholerae/genética
Fatores de Virulência/genética
Vibrio cholerae não O1/genética
Ilhas Genômicas/genética
Proteínas de Ligação a DNA/genética
Sistemas de Secreção Tipo III/genética
-Toxinas Bacterianas/genética
Vibrio cholerae/isolamento & purificação
Vibrio cholerae/patogenicidade
Chile
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Vibrio cholerae não O1/isolamento & purificação
Vibrio cholerae não O1/patogenicidade
Proteínas Hemolisinas/genética
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1179955
Autor: Barros, Felipe Dubourcq.
Título: Avaliação dos níveis de células CD44high/CD24low E TCD3+, e da expressão dos fatores de transcrição SOX2 e STAT3 no carcinoma escamoso de pênis / Evaluation of CD44highCD24low and TCD3 + cell levels, and expression of SOX2 and STAT3 transcription factors in penile squamous cell carcinoma.
Fonte: São Paulo; s.n; 2019. 99 p. ilust.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: Introdução: O câncer de pênis (CaPe) tem uma alta incidência em alguns países subdesenvolvidos. O tratamento padrão é a remoção do tumor primário e a linfadenectomia inguinal, em alguns pacientes. O fator prognóstico mais importante é a doença linfonodal, porém os métodos de estadiamento, disponíveis na atualidade são pouco precisos, e a alta taxa de morbidade da linfadenectomia tem estimulado o estudo de biomarcadores preditivos de metástases em linfonodos, selecionando os pacientes que necessitam da linfadenectomia. Os marcadores STAT3 e CD44, CD24, e SOX2+ são conhecidos por serem considerados marcadores de diagnóstico e prognóstico em outros cânceres, mas sem estudos no câncer de pênis. Objetivo: Avaliar os níveis das células CD44high/CD24low E TCD3+, e a expressão dos fatores de transcrição SOX2 e STAT3 no carcinoma escamoso de pênis. Métodos: Estudo transversal realizado no Hospital de Câncer de Pernambuco (HCP) e Laboratório de Pesquisa Translacional do Instituto de Medicina Integral Prof. Fernando Figueira (IMIP), no período de março de 2015 a dezembro de 2017 em uma população de 38 pacientes com CaPe e controles. Foram realizadas análises de SOX2, STAT3, CD24 e CD44 no sangue e tecido tumoral por citometria de fluxo. Resultados: A mediana da idade foi de 61 anos (37 - 80 anos). A maioria dos pacientes apresentou tumor localizado na glande (76,3%), tipo histológico usual (71,0%), de grau 1 (86,8%), sem invasão vascular e perineural (86,8% e 68,4%), sem presença de metástases (86,8%). Com relação ao estadiamento, 55,3% dos pacientes apresentavam estágio pT2 e 34,2% pT1. Foram encontrados níveis elevados de CD44highCD24low, CD44highCD24lowpSTAT3+ e CD44highCD24lowSOX2+nos pacientes, quando comparados aos controles (p<0,05). Foram observados níveis percentuais reduzidos de leucócitos SOX2+ e TCD3+/SOX2+, e elevados leucócitos pSTAT3 nos pacientes, quando comparados aos dos controles saudáveis. Foram constatados níveis elevados de CD44highCD24low e de leucócitos SOX2+ no grupo de pacientes com invasão perineural, tamanho tumoral > 3 cm e em estádio pT2 (p<0,05). Foram encontradas: associação dos níveis de célulasCD44high/CD24low e no sangue periférico com o tamanho do tumor (p=0,04) e status de sobrevida (p=0,01) e de CD44high/CD24lowSOX2+ no sangue e tumor com status de sobrevida nos pacientes;expressão reduzida de pSTAT3 no sangue de pacientes com invasão perineural e elevados no tecido tumoral no grupo em estádio pT1;alta expressão de TCD3+ no sangue e tecido tumoral de pacientes com tumor ≤3cm;aumento de TCD3+SOX2+ no sangue de pacientes sem invasão perineural e em estádio pT1.Observou-se correlação de CD44highCD24lowpSTAT3+ (r=0,669; p=0,024), de pSTAT3 (r=0,487; p=0,018) e de TCD3+SOX2+ (r=0,404; p=0,029) entre o sangue e o tecido tumoral. Conclusão: As moléculas CD44, CD24 e SOX2 foram marcadores de doença avançada, sendo associados ao pior prognóstico no câncer de pênis, porém, p-STAT3 e TCD3+ foram associados a um prognóstico mais favorável neste estudo

Introduction: Penile cancer (CaPe) has a high incidence in some underdeveloped countries. The standard treatment is removal of the primary tumor and inguinal lymphadenectomy in some patients. At the moment, the most important prognostic factor is lymph node disease, but the staging methods currently available are not very accurate and the high morbidity rate of lymphadenectomy has prompted the study of biomarkers that may predict lymph node metastasis, and aid in the selection of patients who need lymphadenectomy. The markers STAT3 and CD44, CD24, SOX2 + are known to be markers of diagnosis and prognosis in other cancers, but there are no such studies on penile cancer. Aim: To evaluate the levels of CD44high/ CD24low and TCD3+ cells, and the expression of SOX2 and STAT3 transcription factors in squamous cell carcinoma of the penis. Methods: A cross-sectional study was conducted at the Hospital de Cancer de Pernambuco (HCP) and Translational Research Laboratory at the Prof. Fernando Figueira Institute of Integral Medicine (IMIP), from March 2015 to December 2017 of a population of 38 patients with CaPe and controls. Analyses of SOX2, STAT3, CD24 and CD44 were performed on blood and tumor tissue by flow cytometry. Results: The median age was 61 years (37-80 years). Most of the patients presented tumor localized in the glans (76.3%), histological type (71.0%), grade 1 (86.8%), without vascular and perineural invasion (86.8% and 68.4% %), without metastases (86.8%). Regarding staging, 55.3% of the patients had pT2 and 34.2% pT1. High levels of CD44highCD24low, CD44highCD24lowpSTAT3+and CD44highCD24low,SOX2+ were found in the patients compared to the controls (p <0.05). Reduced percentage levels of SOX2+ and TCD3+/ SOX2+ leukocytes and high pSTAT3 leukocytes were observed in the patients when compared to healthy controls. There were elevated levels of CD44highCD24low and SOX2+ leukocytes in the group of patients with perineural invasion, tumor size> 3 cm and at pT2 stage (p <0.05). Peripheral blood CD44highCD24low levels were found to be associated with the tumor size(p = 0.04) and survival status (p = 0.01) and CD44highCD24lowSOX2+ levels in the blood and tumor with survival status in patients. Reduced levels of pSTAT3 in the blood of patients with perineural invasion and elevated tumor tissue in the pT1 stage. There were elevated levels of TCD3 + in the blood and tumor tissue of patients with tumors of ≤3cm. Increased TCD3 + SOX2 + was found in the blood of patients without perineural and pT1 stage invasion. CD44highCD24lowpSTAT3 + (r = 0.669; p = 0.024), pSTAT3 (r = 0.487, p = 0.018) and TCD3 + SOX2 + (r = 0.404, p = 0.029) correlations were observed between blood and tumor tissue. Conclusion: CD44, CD24 and SOX2 molecules were markers of advanced disease, and were associated with the worst prognosis in penile cancer. However, p-STAT3 and TCD3 + were associated with a more favorable prognosis in this study
Descritores: Neoplasias Penianas
Fatores de Transcrição
Linfócitos T
Receptores de Hialuronatos
Fator de Transcrição STAT3
Citometria de Fluxo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Responsável: BR30.1 - Biblioteca
BR30.1



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