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Pesquisa : D12.776.930 [Categoria DeCS]
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Id: biblio-1171325
Autor: Anon.
Título: Resección y regeneración hepática / Hepatic resection and regeneration
Fonte: Rev. argent. cir;(n.esp):132-143, 2000.
Idioma: es.
Conferência: Apresentado em: Congreso Argentino de Cirugía, 71, Buenos Aires, 2000.
Descritores: Hepatectomia/normas
Insuficiência Hepática/etiologia
Regeneração Hepática
-Aminopirina/diagnóstico
Camundongos
Fator de Crescimento Transformador beta
Fator de Crescimento de Hepatócito
Fatores de Transcrição
Fibrose
Insuficiência Hepática/diagnóstico
Insuficiência Hepática/prevenção & controle
/fisiologia
INTERLEUCINA-ABDOMEN, ACUTE/fisiologia
Lipopolissacarídeos
NF-kappa B
Proto-Oncogenes
Regeneração Hepática/fisiologia
Substâncias de Crescimento/fisiologia
Testes de Função Hepática
Verde de Indocianina/diagnóstico
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Id: biblio-983950
Autor: Anwar, Ali; Liu, Yumei; Dong, Rongrong; Bai, Longqiang; Yu, Xianchang; Li, Yansu.
Título: The physiological and molecular mechanism of brassinosteroid in response to stress: a review
Fonte: Biol. Res;51:46, 2018. tab, graf.
Idioma: en.
Projeto: Fundamental Research Funds; . National Key Research and Development Program of China; . Earmarked fund.
Resumo: The negative effects of environmental stresses, such as low temperature, high temperature, salinity, drought, heavy metal stress, and biotic stress significantly decrease crop productivity. Plant hormones are currently being used to induce stress tolerance in a variety of plants. Brassinosteroids (commonly known as BR) are a group of phytohormones that regulate a wide range of biological processes that lead to tolerance of various stresses in plants. BR stimulate BRASSINAZOLE RESISTANCE 1 (BZR1)/BRI1-EMS SUPPRESSOR 1 (BES1), transcription factors that activate thousands of BR-targeted genes. BR regulate antioxidant enzyme activities, chlorophyll contents, photosynthetic capacity, and carbohydrate metabolism to increase plant growth under stress. Mutants with BR defects have shortened root and shoot developments. Exogenous BR application increases the biosynthesis of endogenous hormones such as indole-3-acetic acid, abscisic acid, jasmonic acid, zeatin riboside, brassinosteroids (BR), and isopentenyl adenosine, and gibberellin (GA) and regulates signal transduction pathways to stimulate stress tolerance. This review will describe advancements in knowledge of BR and their roles in response to different stress conditions in plants.
Descritores: Estresse Fisiológico/fisiologia
Fatores de Transcrição/genética
Transdução de Sinais/genética
Regulação da Expressão Gênica de Plantas/genética
Brassinosteroides/metabolismo
-Estresse Fisiológico/genética
Transdução de Sinais/fisiologia
Regulação da Expressão Gênica de Plantas/fisiologia
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1011398
Autor: Aramwit, Pornanong; Luplertlop, Natthanej; Kanjanapruthipong, Tapanee; Ampawong, Sumate.
Título: Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
Fonte: Biol. Res;51:54, 2018. graf.
Idioma: en.
Projeto: Thailand Research Fund (TRF); . Office of the Higher Education Commission (OHEC).
Resumo: BACKGROUND: Hyperpigmentation disorders such as post-inflammatory hyperpigmentation are major concerns not only in light-skinned people but also in Asian populations with darker skin. The anti-tyrosinase and immunomodulatory effects of sericin have been known for decades. However, the therapeutic effects of sericin on hyperpigmentation disorders have not been well documented. METHODS: In this study, we used an in vitro model to study the anti-tyrosinase, tolerogenic, and anti-melanogenic effects of sericin on Staphylococcus aureus peptidoglycan (PEG)-stimulated melanocytes, dendritic cells (DCs), and artificial skin (MelanoDerm™). Enzyme-linked immunosorbent assay, conventional and immunolabeled electron microscopy, and histopathological studies were performed. RESULTS: The results revealed that urea-extracted sericin has strong anti-tyrosinase properties as shown by a reduction of tyrosinase activity in melanin pigments both 48 h and 10 days after allergic induction with PEG. Anti-inflammatory cytokines including interleukin (IL)-4, IL-10, and transforming growth factor-p were upregulated upon sericin treatment (10, 20, and 50 µg/mL), whereas production of allergic chemokines, CCL8 and CCL18, by DCs was diminished 48 h after allergic induction with PEG. Moreover, sericin lowered the expression of micropthalmia-associated transcription factor (MITF), a marker of melanogenesis regulation, in melanocytes and keratinocytes, which contributed to the reduction of melanin size and the magnitude of melanin deposition. However, sericin had no effect on melanin transport between melanocytes and keratinocytes, as demonstrated by a high retention of cytoskeletal components. CONCLUSION: In summary, sericin suppresses melanogenesis by inhibition of tyrosinase activity, reduction of inflammation and allergy, and modulation of MITF function.
Descritores: Queratinócitos/efeitos dos fármacos
Monofenol Mono-Oxigenase/antagonistas & inibidores
Hiperpigmentação/tratamento farmacológico
Sericinas/farmacologia
Melanócitos/efeitos dos fármacos
-Fatores de Transcrição/efeitos dos fármacos
Microscopia Eletrônica
Transdução de Sinais/efeitos dos fármacos
Queratinócitos/ultraestrutura
Células Cultivadas
Fator de Transcrição Associado à Microftalmia
Hipersensibilidade
Inflamação
Melanócitos/ultraestrutura
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1011407
Autor: Yin, Hongtao; Yu, Yan.
Título: Identification of the targets of hematoporphyrin derivative in lung adenocarcinoma using integrated network analysis
Fonte: Biol. Res;52:4, 2019. tab, graf.
Idioma: en.
Resumo: BACKGROUND: Hematoporphyrin derivative (HPD) has a sensibilization effect in lung adenocarcinoma. This study was conducted to identify the target genes of HPD in lung adenocarcinoma. METHODS: RNA sequencing was performed using the lung adenocarcinoma cell line A549 after no treatment or treatment with X-ray or X-ray + HPD. The differentially expressed genes (DEGs) were screened using Mfuzz package by noise-robust soft clustering analysis. Enrichment analysis was carried out using "BioCloud" online tool. Protein-protein interaction (PPI) network and module analyses were performed using Cytoscape software. Using WebGestalt tool and integrated transcription factor platform (ITFP), microRNA target and transcription factor (TF) target pairs were separately predicted. An integrated regulatory network was visualized with Cytoscape software. RESULTS: A total of 815 DEGs in the gene set G1 (continuously dysregulated genes along with changes in processing conditions [untreated-treated with X-ray-X-ray + treated with HPD]) and 464 DEGs in the gene set G2 (significantly dysregulated between X-ray + HPD-treated group and untreated/X-ray-treated group) were screened. The significant module identified from the PPI network for gene set G1 showed that ribosomal protein L3 (RPL3) gene could interact with heat shock protein 90 kDa alpha, class A member 1 (HSP90AA1). TFs AAA domain containing 2 (ATAD2) and protein inhibitor of activated STAT 1 (PIAS1) were separately predicted for the genes in gene set G1 and G2, respectively. In the integrated network for gene set G2, ubiquitin-specific peptidase 25 (USP25) was targeted by miR-200b, miR-200c, and miR-429. CONCLUSION: RPL3, HSP90AA1, ATAD2, and PIAS1 as well as USP25, which is targeted by miR-200b, miR-200c, and miR-429, may be the potential targets of HPD in lung adenocarcinoma.
Descritores: Derivado da Hematoporfirina/farmacologia
Redes Reguladoras de Genes/genética
Adenocarcinoma de Pulmão/genética
Neoplasias Pulmonares/genética
-Proteínas Ribossômicas/efeitos dos fármacos
Proteínas Ribossômicas/genética
Fatores de Transcrição
Análise por Conglomerados
Regulação Neoplásica da Expressão Gênica
Análise de Sequência de RNA
Proteínas de Choque Térmico HSP90/efeitos dos fármacos
Proteínas de Choque Térmico HSP90/genética
Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina/efeitos dos fármacos
Proteínas Modificadoras Pequenas Relacionadas à Ubiquitina/genética
MicroRNAs/metabolismo
Linhagem Celular Tumoral
Proteínas de Ligação a DNA/efeitos dos fármacos
Proteínas de Ligação a DNA/genética
Proteínas Inibidoras de STAT Ativados/efeitos dos fármacos
Proteínas Inibidoras de STAT Ativados/genética
Citometria de Fluxo
ATPases Associadas a Diversas Atividades Celulares/efeitos dos fármacos
ATPases Associadas a Diversas Atividades Celulares/genética
Adenocarcinoma de Pulmão/tratamento farmacológico
Adenocarcinoma de Pulmão/radioterapia
Neoplasias Pulmonares/tratamento farmacológico
Neoplasias Pulmonares/radioterapia
Limites: Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-839397
Autor: Sun, Changzhi; Ouyang, Hong; Luo, Renzhong.
Título: Distinct characteristics of nasal polyps with and without eosinophilia / Diferenças nas características de pólipos nasais com e sem eosinofilia
Fonte: Braz. j. otorhinolaryngol. (Impr.);83(1):66-72, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: Medical Scientific Research Foundation of Guangdong Province.
Resumo: Abstract Introduction Eosinophilic and noneosinophilic Nasal polyps (NPs) are different subtypes of NPs and require different treatment methods. Objective To compare the histologic characteristics, mRNA and protein expression between Nasal Polyps with and without eosinophilia. Methods NPs tissues were obtained from eighty-six NPs patients during surgery. Eosinophilic and noneosinophilic NPs were distinguished according to immunochemical results of the specimen. The histological, mRNA and protein expression features were compared between the two groups. Results In eosinophilic NPs, we observed a significantly higher GATA-3, IL-5, IL-4, IL-13 mRNA and protein expression. In noneosinophilic NPs, IL-17, IL-23 and RORc mRNA and protein expression were increased. Immunohistochemistry tests showed, more mast cells and less neutrophils in eosinophilic NPs compared with noneosinophilic NPs. Eosinophilic NPs patient presented more severe symptom scores when compared to noneosinophilic NPs. Conclusion We demonstrate for the first time that Th2 is the predominant reaction in eosinophilic NPs while Th17 is the predominant reaction in noneosinophilic NPs. Our study may provide new treatment strategy for NPs.

Resumo Introdução Pólipos nasais (PNs) eosinofílicos e não eosinofílicos são diferentes subtipos de PNs e requerem diferentes métodos de tratamento. Objetivo Comparar as características histológicas e a expressão de mRNAs e proteínas entre PNs com e sem eosinofilia. Método Amostras de PNs foram obtidos de 86 pacientes durante a cirurgia. PNs eosinofílicos e não eosinofílicos foram diferenciados segundo os resultados imunoistoquímicos de cada amostra. As características histológicas e de expressão de mRNAs e de proteínas foram comparadas entre os dois grupos. Resultados Em PNs eosinofílicos, observamos uma expressão significativamente maior dos mRNAs e proteínas GATA-3, IL-5, IL-4 e IL-13. Nos PNs não eosinofílicos, aumentou a expressão dos mRNAs e das proteínas IL-17, IL-23 e RORc. Nos testes imunoistoquímicos, observamos maior número de mastócitos e menor número de neutrófilos nos PNs eosinofílicos, em comparação com PNs não eosinofílicos. Os pacientes com PNs eosinofílicos obtiveram escores de sintomas mais graves vs. PNs não eosinofílicos. Conclusão Demonstramos, pela primeira vez, uma reação Th2 predominante em PNs eosinofílicos e uma reação Th17 predominante em PNs não eosinofílicos. Nosso estudo pode proporcionar novas estratégias terapêuticas para a rinossinusite crônica.
Descritores: Sinusite/imunologia
Rinite/imunologia
Pólipos Nasais/imunologia
Eosinófilos/imunologia
-Sinusite/complicações
Fatores de Transcrição
Índice de Gravidade de Doença
RNA Mensageiro/metabolismo
Imuno-Histoquímica
Rinite/complicações
Pólipos Nasais/complicações
Pólipos Nasais/metabolismo
Pólipos Nasais/patologia
Doença Crônica
Citocinas/imunologia
Linfócitos T Auxiliares-Indutores/imunologia
Eosinofilia/complicações
Eosinofilia/metabolismo
Eosinofilia/patologia
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Masculino
Feminino
Adulto
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-889318
Autor: Jiang, Xue; Feng, Lichun; Dai, Baoqiang; Li, Liping; Lu, Weiwei.
Título: Identification of key genes involved in nasopharyngeal carcinoma / Identificação dos principais genes envolvidos no carcinoma nasofaríngeo
Fonte: Braz. j. otorhinolaryngol. (Impr.);83(6):670-676, Nov.-Dec. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Introduction: Nasopharyngeal carcinoma is the most common cancer originating from the nasopharynx. Objective: To study the mechanisms of nasopharyngeal carcinoma, we analyzed GSE12452 microarray data. Methods: GSE12452 was downloaded from the Gene Expression Omnibus database and included 31 nasopharyngeal carcinoma samples and 10 normal nasopharyngeal tissue samples. The differentially expressed genes were screened by ANOVA in the PGS package. Using the BiNGO plugin in Cytoscape and pathway enrichment analysis in the PGS package, functional and pathway enrichment analyses were performed separately to predict potential functions of the differentially expressed genes. Furthermore, Transcription factor-differentially expressed gene pairs were searched, and then the transcription factor-differentially expressed gene regulatory network was visualized using Cytoscape software. Results: A total of 487 genes were screened as differentially expressed genes between the nasopharyngeal carcinoma samples and the normal nasopharyngeal tissue samples. Enrichment analysis indicated that PTGS2 was involved in the regulation of biological process and small cell lung cancer. ZIC2 and OVOL1 may function in nasopharyngeal carcinoma through targeting significantly up-regulated genes (such as PTGS2, FN1, CXCL9 and CXCL10) in the Transcription factor-differentially expressed gene regulatory network (e.g., ZIC2→PTGS2 and OVOL1→CXCL10). Conclusion: PTGS2, FN1, CXCL9, CXCL10, ZIC2 and OVOL1 might play roles in nasopharyngeal carcinoma.

Resumo Introdução: O carcinoma nasofaríngeo é o câncer mais comum originário da nasofaringe. Objetivo: Estudar os mecanismos do câncer de nasofaringe; dados do microarray GSE12452 foram analisados. Método: GSE12452 foi obtido da base de dados Gene Expression Omnibus e inclui 31 amostras de carcinoma nasofaríngeo e 10 amostras de tecido nasofaríngeo normal. Os genes diferencialmente expressos foram analisados por ANOVA no kit PGS. Usando o plugin BiNGO no Cytoscape e análise de enriquecimento da via no kit PGS, análises de enriquecimento funcional e da via foram realizadas separadamente para prever as potenciais funções dos genes diferencialmente expressos. Além disso, os pares Fator de Transcrição - genes diferencialmente expressos foram pesquisados e em seguida a sua rede reguladora foi visualizada usando o programa Cytoscape. Resultados: Um total de 487 genes foram analisados como genes diferencialmente expressos entre as amostras de carcinoma nasofaríngeo e amostras de tecido nasofaríngeo normal. A análise de enriquecimento indicou que PTGS2 estava envolvido na regulação do processo biológico e câncer pulmonar de pequenas células. ZIC2 e OVOL1 podem funcionar no carcinoma nasofaríngeo almejando-se de maneira significativa os genes suprarregulados (como o PTGS2, FN1, CXCL9 e CXCL10) na rede reguladora de fator de transcrição - genes diferencialmente expressos (p.ex., ZIC2→PTGS2 e OVOL1→CXCL10). Conclusão: PTGS2, FN1, CXCL9, CXCL10, ZIC2 e OVOL1 podem desempenhar alguns papéis no carcinoma de nasofaringe.
Descritores: Carcinoma/genética
Expressão Gênica
Neoplasias Nasofaríngeas/genética
-Fatores de Transcrição/genética
Proteínas Nucleares/genética
Carcinoma/patologia
Análise por Conglomerados
Regulação para Baixo
Regulação para Cima
Neoplasias Nasofaríngeas/patologia
Análise de Variância
Perfilação da Expressão Gênica
Bases de Dados Genéticas
Análise em Microsséries
Redes Reguladoras de Genes
Quimiocina CXCL9/genética
Quimiocina CXCL10/genética
Carcinoma Nasofaríngeo
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1132588
Autor: Li, Xin; Zhu, Zhengping; Li, Wei; Wei, Li; Zhao, Baocheng; Hao, Zheng.
Título: Polymorphism in GRHL2 gene may contribute to noise-induced hearing loss susceptibility: a meta-analysis / Polimorfismo no gene GRHL2 pode contribuir para a suscetibilidade à perda auditivainduzida por ruído: uma metanálise
Fonte: Braz. j. otorhinolaryngol. (Impr.);86(3):370-375, May-June 2020. tab, graf.
Idioma: en.
Projeto: Humanities and Social Sciences of Ministry of Education Planning Fund of China.
Resumo: Abstract Instruction: Noise-induced hearing loss is a leading occupational disease caused by gene-environment interaction. The Grainy Like 2, GRHL2, is a candidate gene. In this regard, many studies have evaluated the association between GRHL2 and noise-induced hearing loss, although the results are ambiguous and conflicting. Objective: The purpose of this study was to identify a precise estimation of the association between rs3735715 polymorphism in GRHL2 gene and susceptibility of noise-induced hearing loss. Methods: A comprehensive search was performed to collect data up to July 8, 2018. Finally, 4 eligible articles were included in this meta-analysis comprising 2410 subjects. The pooled odds ratios with 95% confidence intervals were used to evaluate the strength of the association. Results: Significant association was found in the overall population in the dominant model (GA/AA vs. GG, odds ratio = 0.707, 95% confidence interval = 0.594-0.841) and allele model (G allele vs. A allele, odds ratio = 1.189, 95% confidence interval = 1.062-1.333). When stratified by source of the subjects, we also found association between rs3735715 and noise-induced hearing loss risk in the dominant model (GA/AA vs. GG, odds ratio = 0.634, 95% confidence interval = 0.514-0.783) and allele model (G allele vs. A allele, odds ratio = 1.206, 95% confidence interval = 1.054-1.379). Conclusion: Rs3735715 polymorphism in GRHL2 gene may influence the susceptibility of noise-induced hearing loss. Additional large, well-designed and functional studies are needed to confirm this association in different populations.

Resumo Introdução: Perda auditiva induzida por ruído é uma das principais doenças ocupacionais causadas pela interação gene-ambiente. O Grainy Like 2, ou GRHL2 é um gene que tem sido considerado como candidato. Nesse sentido, muitos estudos avaliaram a associação entre o GRHL2 e perda auditiva induzida por ruído, embora os resultados sejam ambíguos e conflitantes. Objetivo: Identificar uma estimativa precisa da associação entre o polimorfismo rs3735715 no gene GRHL2 e a suscetibilidade à perda auditiva induzida por ruído. Método: Uma pesquisa abrangente foi feita para coletar dados até 8 de julho de 2018. No fim, quatro artigos elegíveis foram incluídos nesta metanálise, abrangeram 2.410 indivíduos. As odds ratios agrupadas com intervalos de confiança de 95% foram usadas para avaliar a força da associação. Resultados: Uma associação significante foi encontrada na população geral no modelo de dominância (GA/AA vs. GG, odds ratio = 0,707, intervalo de confiança 95% = 0,594-0,841) e modelo de alelo (alelo G vs. alelo A; odds ratio = 1,189, intervalo de confiança 95% = 1,062 a 1,333). Quando estratificados pelo local de trabalho dos indivíduos, também encontramos associação entre rs3735715 e risco de perda auditiva induzida por ruído no modelo de dominância (GA/AA vs. GG, odds ratio = 0,634, intervalo de confiança 95% = 0,514 ± 0,783) e modelo de alelo (alelo G vs. alelo A; odds ratio = 1,206, intervalo de confiança 95% = 1,054- 1,379). Conclusão: O polimorfismo Rs3735715 no gene GRHL2 pode influenciar a suscetibilidade à perda auditiva induzida por ruído. Estudos adicionais, amplos, bem desenhados e funcionais são necessários para confirmar essa associação em diferentes populações.
Descritores: Fatores de Transcrição/genética
Predisposição Genética para Doença/genética
Polimorfismo de Nucleotídeo Único/genética
Proteínas de Ligação a DNA/genética
Perda Auditiva Provocada por Ruído/genética
-Genótipo
Ruído Ocupacional/efeitos adversos
Doenças Profissionais/genética
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-1052041
Autor: Qu, Jingwen; Guo, Haiyan; Li, Yongjun; Wang, Qiang; Yin, Xiuyuan; Sun, Xiaomei; Ji, Dejun.
Título: Expression of CPEB1 gene affects the cycle of ovarian granulosa cells from adult and young goats
Fonte: Electron. j. biotechnol;39:74-81, may. 2019. tab, ilus, graf.
Idioma: en.
Projeto: Key Natural Science Program of Jiangsu Higher Education Institutions; . Priority Academic Program Development of Jiangsu Higher Education Institutions.
Resumo: Background: CPEB is considered as an RNA-binding protein first identified in Xenopus oocytes. Although CPEB1 was involved in the growth of oocyte, its role in goat follicular granulosa cell has not been fully elucidated. To clarify the functions of this gene in goat follicular granulosa cells, CPEB1-overexpressing vector and interference vector were structured and transfected into follicular granulosa cells from Jiangsu native white goats of Nantong city, Jiangsu Province, China. The expression levels of differentiation-related genes including CDK1, Cyclin B1, and C-mos were determined 24 h after administration of CPEB1 by quantitative real-time polymerase chain reaction and Western blotting methods. Results: The expression levels of CDK1, Cyclin B1, and C-mos were significantly upregulated after overexpression and significantly downregulated after interference with CPEB1. Conclusions: The CPEB1 gene expression could affect the transcription of genes related to early cleavage divisions, which provided a reference for further research on its role in the growth and maturation of oocytes.
Descritores: Oócitos
Fatores de Transcrição/genética
Cabras/genética
-Transfecção
Fertilização In Vitro
Expressão Gênica
Western Blotting
Reação em Cadeia da Polimerase/métodos
Proteínas de Ligação a RNA
Transferência Embrionária
Gado
Fluorescência
Células da Granulosa
Limites: Animais
Feminino
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1052010
Autor: Wu, Qi; Zhao, Gang; Bai, Xue; Zhao, Wei; Xiang, Dabing; Wan, Yan; Wu, Xiaoyong; Sun, Yanxia; Tan, Maoling; Peng, Lianxin; Zhao, Jianglin.
Título: Characterization of the transcriptional profiles in common buckwheat (Fagopyrum esculentum) under PEG-mediated drought stress
Fonte: Electron. j. biotechnol;39:42-51, may. 2019. graf, tab.
Idioma: en.
Projeto: Education Department of Sichuan Province; . National Natural Science Foundation of China; . Sichuan Science and Technology Program; . Agriculture Research System of China; . Chengdu University Research Fund.
Resumo: BACKGROUND: Common buckwheat (Fagopyrum esculentum) is an important staple food crop in southwest China, where drought stress is one of the largest limiting factors that lead to decreased crop production. To reveal the molecular mechanism of common buckwheat in response to drought stress, we performed a comprehensive transcriptomics study to evaluate gene expression profiles of common buckwheat during PEG-mediated drought treatment. RESULTS: In total, 45 million clean reads were assembled into 53,404 unigenes with an average length of 749 bp and N50 length of 1296 bp. A total of 1329 differentially expressed genes (DEGs) were identified by comparing wellwatered and drought-treated plants, out of which 666 were upregulated and 663 were downregulated. Furthermore, we defined the functional characteristics of DEGs using GO and KEGG classifications. GO enrichment analysis showed that the DEGs were significantly overrepresented in four categories, namely, "oxidoreductase activity," "oxidation­reduction process," "xyloglucan:xyloglucosyl transferase activity," and "apoplast." Using KEGG pathway analysis, a large number of annotated genes were overrepresented in terms such as "plant hormone signal transduction," "phenylpropanoid biosynthesis," "photosynthesis," and "carbon metabolism." Conclusions: These results can be further exploited to investigate the molecular mechanism of common buckwheat in response to drought treatment and could supply with valuable molecular sources for abiotic-tolerant elite breeding programs in the future.
Descritores: Estresse Fisiológico/genética
Fagopyrum/genética
-Fatores de Transcrição
Transferases
Transdução de Sinais
Expressão Gênica
Análise de Sequência de RNA
Secas
Proteínas de Ligação à Clorofila
Reação em Cadeia da Polimerase em Tempo Real
Transcriptoma
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1053486
Autor: Lv, Runling; Liu, Yuwei; Gong, Xiaodong; Han, Jianmin; Gu, Shouqin; Dong, Jingao.
Título: Expression and purification of the transcription factor StMsn2 from Setosphaeria turcica in Escherichia coli
Fonte: Electron. j. biotechnol;40:65-70, July. 2019. ilus.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Natural Science Foundation of Hebei Province.
Resumo: Background: In Saccharomyces cerevisiae, Msn2, which acts as a key transcription factor downstream the MAPKHOG cascade pathway, also regulates the expression of genes related to stress responses. However, little is known about the regulation mechanisms of the transcription factor in Setosphaeria turcica. Results: In this study, a zinc finger DNA-binding protein, designated as StMSN2, was cloned from S. turcica. Sequencing results showed that StMSN2 had a 1752 bp open reading frame (ORF), which was interrupted by an intron (135 bp) and encoded a putative 538-amino acid protein. Phylogenetic analysis further revealed that StMsn2 was more closely related to Msn2 of Aspergillus parasiticus. StMSN2 was cloned into the pET-28a vector with His (Histidine) tags and induced with 1 mM IPTG (isopropyl-ß-D-thiogalactoside) at 37°C. The recombinant His-tagged StMsn2 was purified, and a band of size approximately 58.8 kDa was obtained. The high specificity of the polyclonal antibody Msn2-2 was detected with the StMsn2 protein from S. turcica and prokaryotic expression system, respectively. Conclusions: A new gene, named StMSN2, with 1617 bp ORF was cloned from S. turcica and characterized using bioinformatics methods. StMsn2 was expressed and purified in a prokaryotic system. A polyclonal antibody, named Msn2-2, against StMsn2 with high specificity was identified.
Descritores: Doenças das Plantas
Ascomicetos/genética
Ascomicetos/patogenicidade
Fatores de Transcrição/isolamento & purificação
-Ascomicetos/metabolismo
Estresse Fisiológico
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
Proteínas de Transporte
Expressão Gênica
Western Blotting
Fases de Leitura Aberta
Dedos de Zinco
Clonagem Molecular
Zea mays
Escherichia coli
Helminthosporium
Epitopos
Responsável: CL1.1 - Biblioteca Central



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