Base de dados : LILACS
Pesquisa : D12.776.930.780.625 [Categoria DeCS]
Referências encontradas : 2 [refinar]
Mostrando: 1 .. 2   no formato [Detalhado]

página 1 de 1

  1 / 2 LILACS  
              next record last record
para imprimir
Texto completo
Id: biblio-1117078
Autor: Costa, Bruna Pasqualotto; Brum, Ilma Simoni; Pizzolato, Lolita Schneider; Biolchi, Vanderlei; Branchini, Gisele.
Título: PSA secretion and cell proliferation are affected by NCoR1 silencing in prostate cancer cells
Fonte: Clin. biomed. res;40(1):37-43, 2020.
Idioma: en.
Resumo: Introduction: The androgen receptor (AR) plays an important role in normal development of the prostate gland, as well as in prostatic neoplasms. Transcriptional regulation by AR is modulated by its interaction with co-activators or co-repressors, such as NCoR1 (nuclear receptor co-repressor 1), which is involved in reducing AR activity over the target gene transcription. Methods: To identify the role of NCoR1 in the prostate cancer androgen independence in a cell line model, we aimed to evaluate the effects of silencing NCoR1 on prostate-specific antigen (PSA) gene expression, the proliferative response and PSA secretion on the supernatant of C4-2B and LNCaP cells that were submitted to small interfering RNAs (siRNAs) transfection, and to treatments with different androgen dosages. Results: In LNCaP and C4-2B cells with no dihydrotestosterone (DHT) treatment, a decrease in PSA mRNA expression was observed 48 hours and 72 hours after gene silencing in the siNCoR group when compared to the control and siNC groups. The LNCaP and C4-2B cells showed a biphasic pattern in response to dihydrotestosterone treatment in transfected groups (siNCoR and siNC) as well as in the control condition (without transfection). The secretion of PSA in cell supernatant of LNCaP and C4-2B cells was higher in the siNCoR group, and, in relation to hormonal treatment, higher in the 10-8 M DHT group. Conclusions: A reduction in the NCoR1 levels seems to have a double influence on the activity of AR in PCa cells. These results suggest that NCoR may act as an AR co-repressor depending upon hormonal stimulation.(AU)
Descritores: Neoplasias da Próstata
Antígeno Prostático Específico
Proliferação de Células
Correpressor 1 de Receptor Nuclear
Receptores Androgênicos
Linhagem Celular
Proteínas Correpressoras
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR18.1 - Biblioteca FAMED/HCPA

  2 / 2 LILACS  
              first record previous record
para imprimir
Texto completo
Id: biblio-1021557
Autor: Li, Hedan; Hao, Chengwei; Xu, Daqing.
Título: Development of a novel vector for cloning and expressing extremely toxic genes in Escherichia coli
Fonte: Electron. j. biotechnol;30:88-94, nov. 2017. tab, ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Specialized Research Fund for the Doctoral Programme of Higher Education of China.
Resumo: Background: Escherichia coli has been widely used as a host to clone and express heterologous genes. However, there are few vectors available for cloning and expressing extremely toxic genes, which limits further basic and applied research on extremely toxic proteins. Results: In this study, a novel vector pAU10 was constructed in E. coli. pAU10 utilizes the combination of the efficient but highly repressible T7-lacO promoter/operator and the strong rrnBT2 transcriptional terminator upstream of the T7 promoter to strictly control unwanted transcription of the extremely toxic gene; in addition, the trp promoter/operator is oriented opposite to the T7 promoter to control the production of the antisense RNA that may block the translation of leaky mRNA. Without the supplementation of IPTG and L-tryptophan in the culture medium, transcription of the extremely toxic gene by the T7 promoter is highly repressed, and the trp promoter produces the antisense RNA, which strictly prevents unwanted expression of the extremely toxic protein in E. coli. With the supplementation of IPTG and L-tryptophan, the T7 promoter efficiently transcribes the extremely toxic gene, and the trp promoter does not produce the antisense RNA, ensuring efficient expression of the extremely toxic protein in E. coli. Tight regulation and efficiency of expression of an extremely toxic gene cloned in the vector pAU10 were confirmed by cloning and expressing the restriction endonuclease-encoding gene bamHI without its corresponding methylase gene in E. coli JM109(DE3). Conclusion: pAU10 is a good vector used for cloning and expressing extremely toxic genes in E. coli.
Descritores: Proteínas de Escherichia coli/toxicidade
Escherichia coli/genética
Vetores Genéticos
Desoxirribonuclease BamHI/metabolismo
Western Blotting
Reação em Cadeia da Polimerase
RNA Antissenso
Regiões Promotoras Genéticas
Clonagem Molecular
Eletroforese em Gel de Poliacrilamida
Proteínas Correpressoras
Genes Bacterianos
Responsável: CL1.1 - Biblioteca Central

página 1 de 1

Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  

Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde