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Id: biblio-894834
Autor: Souza, Claudemir; Zanchin, Nilson IT; Krieger, Marco A; Ludwig, Adriana.
Título: In silico analysis of amino acid variation in human respiratory syncytial virus: insights into immunodiagnostics
Fonte: Mem. Inst. Oswaldo Cruz;112(10):655-663, Oct. 2017. tab, graf.
Idioma: en.
Projeto: FINEP; . BNDES.
Resumo: BACKGROUND The highly contagious nature of human respiratory syncytial virus (HRSV) and the gravity of its infection in newborns and vulnerable adults pose a serious public health problem. Thus, a rapid and sensitive diagnostic test for viral detection that can be implemented upon the first appearance of symptoms is needed. The genetic variation of the virus must be considered for immunodiagnostic purposes. OBJECTIVES To analyse HRSV genetic variation and discuss the possible consequences for capture immunoassay development. METHODS We performed a wide analysis of N, F and G protein variation based on the HRSV sequences currently available in the GenBank database. We also evaluated their similarity with homologous proteins from other viruses. FINDINGS The mean amino acid divergences for the N, F, and G proteins between HRSV-A and HRSV-B were determined to be approximately 4%, 10% and 47%, respectively. Due to their high conservation, assays based on the full-length N and F proteins may not distinguish HRSV from human metapneumovirus and other Mononegavirales viruses, and the full-length G protein would most likely produce false negative results due to its high divergence. MAIN CONCLUSIONS We have identified specific regions in each of these three proteins that have higher potential to produce specific results, and their combined utilisation should be considered for immunoassay development.
Descritores: Peptídeo Sintases
Vírus Sinciciais Respiratórios
Variação Genética
Proteínas Virais/genética
Genótipo
-Filogenia
Testes Imunológicos
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


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Richtzenhain, Leonardo José
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Id: biblio-889223
Autor: Favaro, Patricia Filippsen; Fernandes, Wilson Roberto; Reischak, Dilmara; Brandão, Paulo Eduardo; Silva, Sheila Oliveira de Souza; Richtzenhain, Leonardo José.
Título: Evolution of equine influenza viruses (H3N8) during a Brazilian outbreak, 2015
Fonte: Braz. j. microbiol;49(2):336-346, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: FAPESP-Brazil; . CNPq-Brazil; . CAPES/PROEX-Brazil.
Resumo: Abstract Equine influenza is one of the major respiratory infectious diseases in horses. An equine influenza virus outbreak was identified in vaccinated and unvaccinated horses in a veterinary school hospital in São Paulo, SP, Brazil, in September 2015. The twelve equine influenza viruses isolated belonged to Florida Clade 1. The hemagglutinin and neuraminidase amino acid sequences were compared with the recent isolates from North and South America and the World Organisation for Animal Health recommended Florida Clade 1 vaccine strain. The hemagglutinin amino acid sequences had nine substitutions, compared with the vaccine strain. Two of them were in antigenic site A (A138S and G142R), one in antigenic site E (R62K) and another not in antigenic site (K304E). The four substitutions changed the hydrophobicity of hemagglutinin. Three distinct genetic variants were identified during the outbreak. Eleven variants were found in four quasispecies, which suggests the equine influenza virus evolved during the outbreak. The use of an out of date vaccine strain or updated vaccines without the production of protective antibody titers might be the major contributing factors on virus dissemination during this outbreak.
Descritores: Variação Genética
Surtos de Doenças
Infecções por Orthomyxoviridae/veterinária
Evolução Molecular
Vírus da Influenza A Subtipo H3N8/isolamento & purificação
Doenças dos Cavalos/epidemiologia
Doenças dos Cavalos/virologia
-Orthomyxoviridae
Proteínas Virais/genética
Brasil/epidemiologia
Análise de Sequência de DNA
Infecções por Orthomyxoviridae/epidemiologia
Infecções por Orthomyxoviridae/virologia
Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética
Substituição de Aminoácidos
Vírus da Influenza A Subtipo H3N8/classificação
Vírus da Influenza A Subtipo H3N8/genética
Genótipo
Cavalos
Hospitais Veterinários
Neuraminidase/genética
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-889146
Autor: Ono, Ekaterina Alexandrovna Durymanova; Taniwaki, Sueli Akemi; Brandão, Paulo.
Título: Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA
Fonte: Braz. j. microbiol;48(3):566-569, July-Sept. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP; . National Counsel of Technological and Scientific Development.
Resumo: Abstract The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1 log decrease in virus titter and 5.16-fold reduction in P mRNA, 24 h post-inoculation when compared to non-treated cells. In vivo, siRNA 360 was able to induce partial protection, but with no significant difference when compared to non-treated mice. These results indicate that, despite the need for improvement for in vivo applications, P mRNA might be a target for an RNAi-based treatment for rabies.
Descritores: Fosfoproteínas/genética
Raiva/veterinária
Vírus da Raiva/genética
Proteínas Virais/genética
Quirópteros/virologia
RNA Interferente Pequeno/genética
Interferência de RNA
-Fosfoproteínas/metabolismo
Raiva/virologia
Vírus da Raiva/fisiologia
Proteínas Virais/metabolismo
Replicação Viral
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/metabolismo
Limites: Animais
Responsável: BR1.1 - BIREME


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Shirata, Neuza K
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Id: biblio-1024785
Autor: Stangherlin, Lucas M; Castro, Fabiane Lucy F; Medeiros, Raphael Salles S; Guerra, Juliana Mariotti; Kimura, Lidia Midori; Shirata, Neuza K; Nonogaki, Suely; Dos Santos, Claudia Januário; Carlan Silva, Maria Cristina.
Título: Human Cytomegalovirus DNA Quantification and Gene Expression in Gliomas of Different Grades
Fonte: Plos one;11(7), 2016.
Idioma: en.
Resumo: Gliomas are the most common type of primary brain tumors. The most aggressive type, Glioblastoma multiforme (GBM), is one of the deadliest human diseases, with an average survival at diagnosis of about 1 year. Previous evidence suggests a link between human cytomegalovirus (HCMV) and gliomas. HCMV has been shown to be present in these tumors and several viral proteins can have oncogenic properties in glioma cells. Here we have investigated the presence of HCMV DNA, RNA and proteins in fifty-two gliomas of different grades of malignancy. The UL83 viral region, the early beta 2.7 RNA and viral protein were detected in 73%, 36% and 57% by qPCR, ISH and IHC, respectively. Positivity of the viral targets and viral load was independent of tumor type or grade suggesting no correlation between viral presence and tumor progression. Our results demonstrate high prevalence of the virus in gliomas from Brazilian patients, contributing to a better understanding of the association between HCMV infection and gliomas worldwide and supporting further investigations of the virus oncomodulatory properties.
Descritores: Proteínas Virais/genética
Brasil
Seres Humanos
RNA Viral
Imuno-Histoquímica
Hibridização In Situ
Infecções por Citomegalovirus/complicações
Carga Viral
Citomegalovirus/genética
Glioma
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-1022299
Autor: Luchs, A; Cilli, A; Morillo, S G; Ribeiro, Cibele Daniel; Carmona, Rita de Cássia C; Timenetsky, M do C S T.
Título: Rotavirus genotypes and the indigenous children of Brazilian midwest in the vaccine era, 2008-2012: footprints of animal genome
Fonte: J. med. virol;87(11):1881-1889, 2015.
Idioma: en.
Resumo: World group A rotavirus (RVA) surveillance data provides useful estimates of the disease burden, however, indigenous population might require special consideration. The aim of this study was to describe the results of G­ and P­types from Brazilian native children ≤3 years. Furthermore, selected strains have been analyzed for the VP7, VP6, VP4, and NSP4 encoding genes in order to gain insight into genetic variability of Brazilian strains. A total of 149 samples, collected during 2008­2012, were tested for RVA using ELISA and PAGE, following by RT­PCR and sequencing. RVA infection was detected in 8.7% of samples (13/149). Genotype G2P[4] was detected in 2008 and 2010, G8P[6] in 2009, and G3P[8] in 2011. The phylogenetic analysis of the VP7 and VP4 genes grouped the Brazilian G2P[4] and G3P[8] strains within the lineages currently circulating in humans worldwide. However, the phylogenetic analysis of the VP6 and NSP4 from the Brazilian G2P[4] strains, and the VP7 and NSP4 from the Brazilian G3P[8] strains suggest a distant common ancestor with different animal strains (bovine, caprine, and porcine). The epidemiological and genetic information obtained in the present study is expected to provide an updated understanding of RVA genotypes circulating in the native infant population, and to formulate policies for the use of RVA vaccines in indigenous Brazilian people. Moreover, these results highlight the great diversity of human RVA strains circulating in Brazil, and an in­depth surveillance of human and animal RVA will lead to a better understanding of the complex dynamics of RVA evolution
Descritores: Filogenia
Infecções por Rotavirus/virologia
Variação Genética
Proteínas Virais/genética
Brasil
Seres Humanos
Ensaio de Imunoadsorção Enzimática
Dados de Sequência Molecular
Pré-Escolar
Reação em Cadeia da Polimerase
Homologia de Sequência
Análise de Sequência de DNA
Rotavirus/isolamento & purificação
Rotavirus/genética
Rotavirus/química
Evolução Molecular
Grupos Populacionais
Genótipo
Lactente
Responsável: BR91.2 - Centro de Documentação


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Id: biblio-971493
Autor: Ali, Sádia Abdul Remane Amade.
Título: Caracterização genética dos vírus do sarampo genótipo D4 detectados no Brasil no período de 2003-2012 / Genetic characterisation of measles virus genotype D4 detected in Brazil, 2003-2012.
Fonte: Rio de Janeiro; s.n; 2012. xviii, 133 p. map, tab, graf.
Idioma: pt.
Tese: Apresentada a Instituto Oswaldo Cruz para obtenção do grau de Mestre.
Resumo: O sarampo é uma doença exantemática viral, altamente infecciosa, causada por um vírus da família Paramyxoviridae, gênero Morbillivirus que, apesar de estar disponível uma vacina, segura e eficaz contra a doença, esta ainda constitui uma importante causa de morbidade e mortalidade infantil em muitos países em desenvolvimento. Embora exista apenas um tipo antigênico do vírus do sarampo, estudos de caracterização genética dos vírus de tipo selvagem permitiram a identificação oito subtipos (A-H) e 24 genótipos. O Brasil eliminou a transmissão autóctone do vírus do sarampo a partir do ano 2000. A partir de então foram confirmados vários casos de sarampo importados ou relacionados a importações, principalmente do genótipo D4. A epidemiologia molecular dos vírus do sarampo baseada nas análises da região C-terminal da nucleoproteína tem demonstrado uma diversidade limitada entre as cepas circulantes, dificultando dessa forma a determinação da origem dos vírus usando apenas essa região. O objetivo deste trabalho foi caracterizar geneticamente os genótipos D4 dos vírus do sarampo detectados no Brasil no período de 2003 a 2012, e para tal, as sequências da proteína H completa e do gene N parcial foram analisadas. Os casos positivos para o genótipo D4 foram previamente identificados pela amplificação dos 450nt da região C-terminal da proteína N por RT-PCR, as sequências completas do gene H destas amostras foram diretamente amplificadas por RT-PCR a partir das amostras clínicas e posteriormente sequenciado.As análises filogenéticas da sequência de nucleotídeos do gene N e do gene H completomos traram que os vírus do sarampo genótipo D4 detectados no Brasil podem ser resultado de várias importações de diferentes variantes do mesmo genótipo que circulam na Europa. Foram identificadas mutações nas sequências de aminoácidos tantodo gene N parcial como do gene H completo dos genótipos D4 dos vírus do sarampo detectados no Brasil...

Measles is a highly infectious viral exanthem of the family Paramyxoviridae, genusMorbillivirus. Despite the availability of a safe and effective vaccine, measles infectioncontinues to be an important cause of infantile morbidity and mortality in developingcountries. Although there is only one antigenic type of the measles virus, geneticcharacterisation of the wild-type virus identified 8 subtypes (A-H), with a total of 24genotypes being recognised. From 2000, the indigenous transmission of the measlesvirus has been eliminated in Brazil. Since then, several cases of imported measles, orcases associated with importations, were confirmed, principally of genotype D4. Themolecular epidemiology of the measles virus based on analyses of the C-terminal regionof the nucleoprotein has demonstrated a limited diversity between circulating strains,making it difficult to determine the origin of the virus by this genomic region alone. Theobjective of this study was to genetically characterise the D4 genotype of measlesviruses detected in Brazil from 2003 to 2012 by analysing sequences from the completeH gene and partial N gene of the virus. Cases positive for measles genotype D4 werepreviously identified by the amplification of a 450nt of the C-terminal region of the Nprotein by RT-PCR. The complete H gene from these samples was amplified by RTPCRdirectly from clinical samples and subsequently sequenced. Phylogenetic analysisof the nucleotide sequences of the N gene and the complete H gene demonstrated thatthe measles D4 genotype detected in Brazil may have been a result of severalimportations of different variants of the same genotype circulating in Europe...
Descritores: Vírus do Sarampo
Hemaglutininas Virais
Proteínas Virais
Limites: Seres Humanos
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas
BR15.1


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Id: biblio-907480
Autor: Pájaro-Castro, Nerlis; Flechas, María C; Ocasionez, Raquel; Stashenko, Elena; Olivero-Verbel, Jesús.
Título: Potential interaction of components from essential oils with dengue virus proteins / Potencial interacción de componentes de aceites esenciales con proteínas del virus del dengue
Fonte: Bol. latinoam. Caribe plantas med. aromát;14(3):141-155, mayo 2015. ilus, tab, graf.
Idioma: en.
Projeto: Colombian government.
Resumo: An antiviral drug for treatment of dengue is an urgent necessity. In this study in silico activities of essential oils components on dengue virus (DENV) were evaluated, and beta-Caryophyllene was subjected to biological examination to assess inhibition of DENV-2 replication. Components previously optimized were coupled with viral proteins prepared, using AutoDock Vina. Theoretical affinity values varied between -4.0 and -7.3 kcal/mol. alpha-copaene, beta-bourbonene, germacrene D, spathulenol, beta-caryophyllene, caryophyllene oxide and (+)- epi-bicyclosesquiphellandrene showed the greatest interaction with viral proteins. beta-caryophyllene inhibits DENV-2 in vitro (50 percent inhibitory concentration [IC50] = 22.5 +/- 5.6 uM [4.6 +/- 1.1 ug/mL] and resulted non-cytostatic with a selectivity index value of 71.1. The in silico results permit infer that DENV proteins are potential targets for the concomitant docking of various essential oils components. Biological examination suggest that beta-caryophyllene acts on very early steps of the viral replication cycle and it might prove virucidal.

Una droga antiviral para tratamiento del dengue es una necesidad urgente. En este estudio se evaluó la actividad in silico de componentes de aceites esenciales sobre el virus del dengue (VDEN) y el beta-cariofileno se seleccionó para evaluar la inhibición sobre la replicación in vitro del VDEN-2. Los componentes previamente optimizados fueron acoplados con proteínas virales preparadas, utilizando AutoDock Vina. Los valores de afinidad variaron entre -4.0 and -7.3 kcal/mol. alfa-Copaeno, beta-bourboneno, germacreno D, spatulenol, beta- cariofileno, óxido de cariofileno y (+)-epi-biciclosesquifellandreno presentaron la mayor interacción con las proteínas virales. beta-Cariofileno inhibió VDEN-2 in vitro (Concentración inhibitoria 50 [IC50] = 22.5 +/- 5.6 uM [4.6 +/- 1.1 ug/mL] y resultó no-citostático con índice de selectividad de 71.1. Los resultados in silico indican que proteínas del VDEN son blancos potenciales para varios componentes. El análisis biológico sugiere que el beta-cariofileno actúa en etapas tempranas de la replicación viral y podría ser virucida.
Descritores: Antivirais/farmacologia
Vírus da Dengue
Óleos Voláteis/farmacologia
Sesquiterpenos/farmacologia
Proteínas Virais
-Simulação de Acoplamento Molecular
Limites: Seres Humanos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-829259
Autor: Pontoriero, Andrea; Avaro, Martín; Benedetti, Estefania; Russo, Mara; Czech, Andrea; Periolo, Natalia; Campos, Ana; Zamora, Ana; Baumeister, Elsa.
Título: Surveillance of antiviral resistance markers in Argentina: detection of E119V neuraminidase mutation in a post-treatment immunocompromised patient
Fonte: Mem. Inst. Oswaldo Cruz;111(12):745-749, Dec. 2016. graf.
Idioma: en.
Projeto: National Agency of Scientific Research, Technology and Innovation of Argentina.
Resumo: Although vaccines are the best means of protection against influenza, neuraminidase inhibitors are currently the main antiviral treatment available to control severe influenza cases. One of the most frequent substitutions in the neuraminidase (NA) protein of influenza A(H3N2) viruses during or soon after oseltamivir administration is E119V mutation. We describe the emergence of a mixed viral population with the E119E/V mutation in the NA protein sequence in a post-treatment influenza sample collected from an immunocompromised patient in Argentina. This substitution was identified by a real-time reverse transcriptase polymerase chain reaction (RT-PCR) protocol and was confirmed by direct Sanger sequencing of the original sample. In 2014, out of 1140 influenza samples received at the National Influenza Centre, 888 samples (78%) were A(H3N2) strains, 244 (21.3%) were type B strains, and 8 (0.7%) were A(H1N1)pdm09 strains. Out of 888 A(H3N2) samples, 842 were tested for the E119V substitution by quantitative RT-PCR: 841 A(H3N2) samples had the wild-type E119 genotype and in one sample, a mixture of viral E119/ V119 subpopulations was detected. Influenza virus surveillance and antiviral resistance studies can lead to better decisions in health policies and help in medical treatment planning, especially for severe cases and immunocompromised patients.
Descritores: Antivirais/uso terapêutico
Vírus da Influenza A Subtipo H3N2/efeitos dos fármacos
Influenza Humana/epidemiologia
Influenza Humana/virologia
Neuraminidase/genética
Oseltamivir/uso terapêutico
Proteínas Virais/genética
-Argentina/epidemiologia
Hospedeiro Imunocomprometido
Vírus da Influenza A Subtipo H3N2
Influenza Humana/tratamento farmacológico
Mutação
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: lil-788999
Autor: Silva Junior, Haroldo Cid da; Pestana, Cristiane Pinheiro; Galler, Ricardo; Medeiros, Marco Alberto.
Título: Solubility as a limiting factor for expression of hepatitis A virus proteins in insect cell-baculovirus system
Fonte: Mem. Inst. Oswaldo Cruz;111(8):535-538, Aug. 2016. graf.
Idioma: en.
Resumo: The use of recombinant proteins may represent an alternative model to inactivated vaccines against hepatitis A virus (HAV). The present study aimed to express the VP1 protein of HAV in baculovirus expression vector system (BEVS). The VP1 was expressed intracellularly with molecular mass of 35 kDa. The VP1 was detected both in the soluble fraction and in the insoluble fraction of the lysate. The extracellular expression of VP1 was also attempted, but the protein remained inside the cell. To verify if hydrophobic characteristics would also be present in the HAV structural polyprotein, the expression of P1-2A protein was evaluated. The P1-2A polyprotein remained insoluble in the cellular extract, even in the early infection stages. These results suggest that HAV structural proteins are prone to form insoluble aggregates. The low solubility represents a drawback for production of large amounts of HAV proteins in BEVS.
Descritores: Baculoviridae/química
Baculoviridae/metabolismo
Vírus da Hepatite A/química
Proteínas Virais/biossíntese
-Baculoviridae/genética
Regulação Viral da Expressão Gênica
Vetores Genéticos
Processamento de Proteína Pós-Traducional
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Solubilidade
Proteínas Virais/química
Proteínas Virais/genética
Responsável: BR1.1 - BIREME


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Id: lil-774236
Autor: Silva, Juliana Fernandes Amorim da.
Título: Camundongos inoculados com DENV2 por via intracerebralhistopatologia, detecção viral e avaliação de proteção mediada por uma vacina de DNA / Mice intracerebrally inoculated with DENV2: histopathology, virus detection and evaluation of protection mediated by a DNA vaccine.
Fonte: Rio de Janeiro; s.n; 2015. xx,163 p. ilus, graf, mapas.
Idioma: pt.
Tese: Apresentada a Instituto Oswaldo Cruz para obtenção do grau de Mestre.
Resumo: A dengue constitui um sério problema de saúde pública, principalmente em regiões tropicais e subtropicais do mundo. [...] Apesar disso, um dos modelos mais utilizados para testes de vacinas contra a dengue se baseia na inoculação em camundongos por via intracerebral (i.c.) de vírus neuroadaptado. No entanto, poucos estudos avaliaram o efeito da infecção i.c. e/ou proteção gerada por protótipos vacinais em diferentes órgãos, tais como fígado, um dosórgãos comprometidos pela dengue em humanos. O nosso grupo construiu a vacina de DNA, pcTPANS1, que induziu altos níveis de sobrevivência em camundongos desafiados por via i.c. com vírus da dengue 2 (DENV2). Diante disso, o presente trabalho se propõe avaliar aspectos da patogênese no cérebro, cerebelo, fígado e pulmão, no modelo de camundongos BALB/c inoculados pela via i.c. com uma dose letal de DENV2, em diferentes dias após infecção (d.p.i.). Adicionalmente, tais análises foram estendidas para animais imunizados com a vacina pcTPANS1 e desafiados com DENV2. Detectamos alterações histopatológicas no cérebro/cerebelo (edema,hemorragia, gliose reacional, microglia hiperplásica e hipertrofiada e infiltrado mononuclear na pia-máter, no neurópilo e perivasculares), no fígado (edema,hemorragia, balonização hepatocitária, infiltrado mononuclear, hiperplasia e hipertrofiade células de Kupffer) e no pulmão (edema, hemorragia, infiltrados mononuclear esperibronquiolares, aumento do número de macrófagos alveolares e espessamento de septo interalveolar). Alguns destes danos foram quantificados, utilizando uma escala subjetiva com atribuição de diferentes graus, revelando diferenças significativas...

The dengue is a serious public health problem, mainly in tropical and subtropicalregions of the world. [...] Nevertheless, oneof the most widely used model for vaccine tests against dengue is based on theinoculation of mice by the intracerebral route (i.c.) with neuroadapted virus. However,few studies have evaluated the effects of i.c. infection and/or protection generated byvaccine prototypes in different organs, such as the liver, one of the compromised organin dengue in humans. Our group have constructed a DNA vaccine, pcTPANS1, whichinduced high survival rates in mice challenged by the i.c. inoculation with dengue virus2 (DENV2). Therefore, the present work aim to evaluate aspects of the pathogenesis inthe brain, cerebellum, liver and lung in the BALB/c mouse model intracerebrallyinoculated with a lethal dose of DENV2, at different days post infection (d.p.i.). Inaddition, these analyzes were extended to pcTPANS1-immunized animals challengedwith DENV2. We detect histopathological changes in the brain/cerebellum(hemorrhage, edema, reactive gliosis, hyperplasic and hypertrophied microglia andmononuclear infiltrate in the pia-mater, neuropile and perivascular), the liver(hemorrhage, edema, hepatocyte ballooning, mononuclear infiltrate, hyperplasia andhypertrophy of Kupffer cells) and the lung (hemorrhage, edema, peribronchialmononuclear infiltrates, increased number of alveolar macrophages and thickening ofinteralveolar septa). Some of these damages were quantified using a subjective scalewith different degrees and revealing significant differences...
Descritores: Dengue/epidemiologia
Vacinas de DNA
Proteínas Virais
Replicação Viral
Vírus da Dengue/fisiologia
-Sistema Nervoso Central
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Camundongos
Tipo de Publ: Estudos de Avaliação
Responsável: BR15.1 - Biblioteca de Ciências Biomédicas



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