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Id: lil-776471
Autor: Ding, Feng; Miao, Xi-Li; Li, Yan-Xia; Dai, Jin-Fen; Yu, Hong-Gang.
Título: Mutations in the S gene and in the overlapping reverse transcriptase region in chronic hepatitis B Chinese patients with coexistence of HBsAg and anti-HBs
Fonte: Braz. j. infect. dis;20(1):1-7, Jan.-Feb. 2016. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: Abstract Background The mechanism underlying the coexistence of hepatitis B surface antigen and antibodies to HBsAg in chronic hepatitis B patients remains unknown. Aims This research aimed to determine the clinical and virological features of the rare pattern. Methods A total of 32 chronic hepatitis B patients infected by HBV genotype C were included: 15 carrying both HBsAg and anti-HBs (group I) and 17 solely positive for HBsAg (group II). S gene and reverse transcriptase region sequences were amplified, sequenced and compared with the reference sequences. Results The amino acid variability within major hydrophilic region, especially the “a” determinant region, and within reverse transcriptase for regions overlapping the major hydrophilic region in group I is significantly higher than those in group II. Mutation sI126S/T within the “a” determinant was the most frequent change, and only patients from group I had the sQ129R, sG130N, sF134I, sG145R amino acid changes, which are known to alter immunogenicity. Conclusions In chronic patients, the concurrent HBsAg/anti-HBs serological profile is associated with an increased aa variability in several key areas of HBV genome. Additional research on these genetic mutants are needed to clarify their biological significance for viral persistence.
Descritores: Anticorpos Anti-Hepatite B/sangue
Antígenos de Superfície da Hepatite B/sangue
Vírus da Hepatite B/genética
Hepatite B Crônica/genética
Hepatite B Crônica/imunologia
DNA Polimerase Dirigida por RNA/genética
Proteínas do Envelope Viral/genética
-China
DNA Viral
Genótipo
Vírus da Hepatite B/imunologia
Mutação
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Limites: Adulto
Feminino
Humanos
Masculino
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-763100
Autor: Hoyos-López, Richard; Soto, Sandra Uribe; Rúa-Uribe, Guillermo; Gallego-Gómez, Juan Carlos.
Título: Molecular identification of Saint Louis encephalitis virus genotype IV in Colombia
Fonte: Mem. Inst. Oswaldo Cruz;110(6):719-725, Sept. 2015. tab, graf.
Idioma: en.
Projeto: COLCIENCIAS.
Resumo: Saint Louis encephalitis virus (SLEV) is a member of the Japanese-encephalitis virus serocomplex of the genus Flavivirus. SLEV is broadly distributed in the Americas and the Caribbean Islands, where it is usually transmitted by mosquitoes of the genus Culex and primarily to birds and mammalian-hosts. Humans are occasionally infected by the virus and are dead-end hosts. SLEV causes encephalitis in temperate regions, while in tropical regions of the Americas, several human cases and a wide biological diversity of SLEV-strains have been reported. The phylogenetic analysis of the envelope (E) protein genes indicated eight-genotypes of SLEV with geographic overlap. The present paper describes the genotyping of two SLEV viruses detected in mosquito-pools collected in northern Colombia (department of Cordoba). We used reverse transcription-polymerase chain reaction to amplify a fragment of theE-gene to confirm the virus identity and completeE-gene sequencing for phylogenetic analysis and genotyping of the two-SLEV viruses found circulating in Córdoba. This is the first report of SLEV genotype IV in Colombia (Córdoba) in mosquitoes from a region of human inhabitation, implicating the risk of human disease due to SLEV infection. Physicians should consider SLEV as a possible aetiology for undiagnosed febrile and neurologic syndromes among their patients who report exposure to mosquito-bites.
Descritores: Culicidae/virologia
Vírus da Encefalite de St. Louis/genética
Proteínas do Envelope Viral/genética
-Colômbia
Sequência Consenso
Código de Barras de DNA Taxonômico
Monitoramento Epidemiológico
Vírus da Encefalite de St. Louis/classificação
Genótipo
Filogenia
Polimorfismo Genético/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Alinhamento de Sequência
Limites: Animais
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-759282
Autor: Zheng, Qi; Xu, Jun; Gao, Huihui; Tao, Ran; Li, Wei; Shang, Shiqiang; Gu, Weizhong.
Título: Receptor expression and responsiveness of human peripheral blood mononuclear cells to a human cytomegalovirus encoded CC chemokine
Fonte: Braz. j. infect. dis;19(4):403-409, July-Aug. 2015. tab, ilus.
Idioma: en.
Projeto: National Science and Technology Support Program of China; . Natural Science Foundation of Zhejiang Province.
Resumo: Human cytomegalovirus is a ubiquitous pathogen that infects the majority of the world's population. After long period of time co-evolving with human being, this pathogen has developed several strategies to evade host immune surveillance. One of the major trick is encoding homologous to those of the host organism or stealing host cellular genes that have significant functions in immune system. To date, we have found several viral immune analogous which include G protein coupled receptor, class I major histocompatibility complex and chemokine. Chemokine is a small group of molecules which is defined by the presence of four cysteines in highly conserved region. The four kinds of chemokines (C, CC, CXC, and CX3C) are classified based on the arrangement of 1 or 2 N-terminal cysteine residues. UL128 protein is one of the analogous that encoded by human cytomegalovirus that has similar amino acid sequences to the human CC chemokine. It has been proved to be one of the essential particles that involved in human cytomegalovirus entry into epithelial/endothelial cells as well as macrophages. It is also the target of potent neutralizing antibodies in human cytomegalovirus-seropositive individuals. We had demonstrated the chemotactic trait of UL128 protein in our previous study. Recombinant UL128 in vitrohas the ability to attract monocytes to the infection region and enhances peripheral blood mononuclear cell proliferation by activating the MAPK/ERK signaling pathway. However, the way that this viral encoded chemokine interacting with peripheral blood mononuclear cells and the detailed mechanism that involving the virus entry into host cells keeps unknown. Here we performed in vitroinvestigation into the effects of UL128 protein on peripheral blood mononuclear cell's activation and receptor binding, which may help us further understand the immunomodulatory function of UL128 protein as well as human cytomegalovirus diffusion mechanism.
Descritores: Quimiocinas CC
Citomegalovirus
Regulação Viral da Expressão Gênica/genética
Leucócitos Mononucleares/virologia
Glicoproteínas de Membrana/imunologia
Proteínas do Envelope Viral/imunologia
-Células Cultivadas
Reagentes para Ligações Cruzadas
Quimiocinas CC/genética
Quimiocinas CC/imunologia
Citomegalovirus/genética
Citomegalovirus/imunologia
Receptores de Quimiocinas/genética
Proteínas Recombinantes/imunologia
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-749973
Autor: Celis, Argelia; Moros, Zoila; Gerder, Marlene; Pagano, Francesca; Vizzi, Esmeralda; Liprandi, Ferdinando.
Título: Selección de variantes de virus dengue sensibles a la heparina en células BHK-21 / Selection of heparin-sensitive dengue virus variants in BHK-21 cells
Fonte: Invest. clín;55(2):155-167, jun. 2014. ilus, tab.
Idioma: es.
Resumo: Estudios previos han demostrado que la adaptación de diversos virus a crecer en líneas celulares de vertebrados, conduce a la selección de variantes virales que unen al heparán sulfato (HS) con alta afinidad. En el presente trabajo se determinó la susceptibilidad de cepas del virus dengue (DENV) a la heparina hipersulfatada un análogo al HS, después de pases seriados en células BHK-21. A aislados de campo de los cuatro serotipos de DENV, se les realizaron ocho pases seriados en células BHK-21. La adaptación de los DENV al cultivo celular seleccionó variantes virales con una aumentada capacidad replicativa en células BHK-21 y una incrementada susceptibilidad a la heparina, en relación a las respectivas cepas no adaptadas, obteniéndose una inhibición de la infectividad más significativa en DENV-2 y DENV-4. Las cepas de DENV adaptadas presentaron cambios en la secuencia de aminoácidos de la proteína de envoltura (E), en particular una substitución K204R para DENV-1, N67K para DENV-2, K308R y V452A para DENV-3 y E327G para DENV-4. Estas sustituciones implicaron ganancia de residuos básicos que incrementaron la carga neta positiva de la proteína. Los resultados sugieren, que la adaptación de cepas de DENV a células BHK-21 selecciona variantes virales sensibles a la heparina y que la efectividad de este compuesto varía dependiendo de la cepa viral. Además sugieren que el HS puede jugar un papel importante en la infectividad de las cepas de DENV adaptadas al cultivo celular, a diferencia de los aislados de DENV no adaptados.

Several studies have shown that adaptation of various viruses to grow in certain cell lines of vertebrates, leads to the selection of virus variants that bind heparan sulfate (HS) with high affinity. In this study we investigated the susceptibility of strains of dengue virus (DENV) to oversulfated heparin an analogue of HS after passages in BHK-21 cells. Field isolates of the four serotypes of DENV with a limited number of passes in mosquito cells C6/36HT were serially passaged eight times in BHK-21 cells. The adaptation of the DENV to the cell culture selected viral variants with an increased replicative capacity in BHK-21 cells and an increased susceptibility to heparin compared with the original not adapted strains, with a more significant inhibition of the infectivity in DENV-2 and DENV-4.The E protein of the adapted strains showed changes in the amino acid sequence, particularly at the position K204R to DENV-1, N67K to DENV-2, K308R and V452A for DENV-3 and E327G to DENV-4. These substitutions implicated a gain of basic residues that increased the net positive charge of the protein. These results suggest that adaptation of DENV strains to BHK-21 cells implies changes in the envelope protein, changes associated to the protein reactivity with heparin, the inhibitory effectiveness of this compound varying depending on the viral strain. In addition, these results suggest that the HS can play an important role in the infectivity of the DENV strains adapted to vertebrate cell culture, but not in the infectivity of non-adapted DENV isolates.
Descritores: Vírus da Dengue/efeitos dos fármacos
Heparina/farmacologia
Seleção Genética
Proteínas do Envelope Viral/genética
-Aedes/citologia
Linhagem Celular
Chlorocebus aethiops
Vírus da Dengue/crescimento & desenvolvimento
Rim/citologia
Mesocricetus
Modelos Moleculares
Mutação
Mutação de Sentido Incorreto
Ligação Proteica
Conformação Proteica
RNA Viral/genética
Análise de Sequência de RNA
Células Vero
Ensaio de Placa Viral
Cultura de Vírus
Replicação Viral
Proteínas do Envelope Viral/química
Proteínas do Envelope Viral/fisiologia
Limites: Animais
Cricetinae
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: VE1.1 - Biblioteca Humberto Garcia Arocha


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Id: lil-748872
Autor: Urbaczek, Ana Carolina; Ximenes, Valdecir Farias; Afonso, Ana; Generoso, Wesley Cardoso; Nogueira, Camila Tita; Tansini, Aline; Cappelini, Luciana Teresa Dias; Malagó Júnior, Wilson; Silva, Flávio Henrique da; Fonseca, Luiz Marcos da; Costa, Paulo Inácio da.
Título: Recombinant hepatitis C virus-envelope protein 2 interactions with low-density lipoprotein/CD81 receptors
Fonte: Mem. Inst. Oswaldo Cruz;110(4):534-542, 09/06/2015. graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Hepatitis C virus (HCV) envelope protein 2 (E2) is involved in viral binding to host cells. The aim of this work was to produce recombinant E2B and E2Y HCV proteins in Escherichia coli and Pichia pastoris, respectively, and to study their interactions with low-density lipoprotein receptor (LDLr) and CD81 in human umbilical vein endothelial cells (HUVEC) and the ECV304 bladder carcinoma cell line. To investigate the effects of human LDL and differences in protein structure (glycosylated or not) on binding efficiency, the recombinant proteins were either associated or not associated with lipoproteins before being assayed. The immunoreactivity of the recombinant proteins was analysed using pooled serum samples that were either positive or negative for hepatitis C. The cells were immunophenotyped by LDLr and CD81 using flow cytometry. Binding and binding inhibition assays were performed in the presence of LDL, foetal bovine serum (FCS) and specific antibodies. The results revealed that binding was reduced in the absence of FCS, but that the addition of human LDL rescued and increased binding capacity. In HUVEC cells, the use of antibodies to block LDLr led to a significant reduction in the binding of E2B and E2Y. CD81 antibodies did not affect E2B and E2Y binding. In ECV304 cells, blocking LDLr and CD81 produced similar effects, but they were not as marked as those that were observed in HUVEC cells. In conclusion, recombinant HCV E2 is dependent on LDL for its ability to bind to LDLr in HUVEC and ECV304 cells. These findings are relevant because E2 acts to anchor HCV to host cells; therefore, high blood levels of LDL could enhance viral infectivity in chronic hepatitis C patients.
Descritores: /fisiologia
ANTIGENS, CDACETABULUM/fisiologia
Células Endoteliais/virologia
Hepacivirus/imunologia
Receptores de LDL/fisiologia
Proteínas do Envelope Viral/fisiologia
-/imunologia
ANTIGENS, CDACETABULUM/imunologia
Linhagem Celular
Escherichia coli
Células Endoteliais/imunologia
Citometria de Fluxo
Proteínas de Membrana
Pichia
Proteínas Recombinantes
Receptores de LDL/imunologia
Limites: Animais
Bovinos
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-731224
Autor: Cassiani, Silvia Helena De Bortoli; Zug, Keri Elizabeth.
Título: Promoting the Advanced Nursing Practice role in Latin America / Promovendo o papel da Prática Avançada de Enfermagem na América Latina / Fomentando el papel de la Práctica Avanzada de Enfermería en América Latina
Fonte: Rev. bras. enferm;67(5):673-674, Sep-Oct/2014.
Idioma: en.
Descritores: Hepacivirus/genética
Hepacivirus/metabolismo
Proteínas do Envelope Viral/química
Proteínas do Envelope Viral/genética
-Linhagem Celular
Glicosilação
Células HeLa
Anticorpos Anti-Hepatite C
Hepacivirus/imunologia
Peso Molecular
Processamento de Proteína Pós-Traducional
Proteínas do Envelope Viral/metabolismo
Limites: Humanos
Animais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-731223
Autor: Malta, Daniela Vieira; Pereira, Laís de Araújo; Santos, Tânia Cristina Franco.
Título: Notícias do Levantamento de Recursos e Necessidades de Enfermagem na Revista Brasileira de Enfermagem (1955-1958) / News of the Inquiry about Nursing Needs and Resources in the Brazilian Journal of Nursing (1955-1958) / Noticias del Levantamiento de Necesidades y Recursos de Enfermería en la Revista Brasileña de Enfermería (1955-1958)
Fonte: Rev. bras. enferm;67(5):679-683, Sep-Oct/2014.
Idioma: pt.
Resumo: Estudo Histórico Social que tem como objeto notícias sobre o Levantamento de Recursos e Necessidades de Enfermagem no Brasil, publicadas na Revista Brasileira de Enfermagem entre 1955 e 1958. A fonte primária foi constituída pelos exemplares da Revista Brasileira de Enfermagem, publicados dentro do recorte temporal do estudo. As fontes secundárias foram constituídas por livros, artigos, dissertações e teses relativas à história da Enfermagem. A análise dos dados teve apoio das fontes secundárias e do pensamento do sociólogo Pierre Bourdieu. Os dados evidenciaram que a Revista Brasileira de Enfermagem, além de oportunizar a divulgação de notícias acerca do Levantamento, proporcionou visibilidade ao mesmo mediante a veiculação dessas notícias e, por fim, teve o efeito simbólico de conferir poder e prestígio à Enfermagem Brasileira.

Social historical study that has as object news related to the Assessment of the Resources and Needs of Nursing in Brazil published in the Revista Brasileira de Enfermagem between 1955 and 1958. The primary source is constituted of copies of Revista Brasileira de Enfermagem published within the selected period of the study. The secondary sources are constituted of books, papers, dissertations and thesis related to the Nursing history. The data analysis was supported by the secondary sources and the thought of the sociologist Pierre Bourdieu. The results evidenced that Revista Brasileira de Enfermagem, in addition to making possible the dissemination of news about the Assessment provided visibility to it and, at last, had the symbolic effect of giving power and prestige to the Brazilian Nursing.

Estudio Histórico Social que tiene como objeto noticias referentes al Levantamiento de Recursos y Necesidades de Enfermería en Brasil publicadas en la Revista Brasileira de Enfermagem entre 1955 y 1958. La fuente primaria se constituye de los ejemplares de la Revista Brasileira de Enfermagem publicados dentro del recorte temporal do estudio. Las fuentes secundarias están constituidas de libros, artículos disertaciones y tesis relativas a la historia de la Enfermería. El análisis de los datos tuvo apoyo de las fuentes secundarias y del pensamiento del Sociólogo Pierre Bourdieu. Los resultados evidencian que la Revista Brasileira de Enfermagem, además de posibilitar la divulgación de noticias acerca del Levantamiento proporcionó visibilidad al mismo mediante la divulgación de esas noticias y, por fin, tuve el efecto simbólico de conferir poder y prestigio a la Enfermería Brasileña.
Descritores: Antígenos de Superfície da Hepatite B/genética
Vacinas contra Hepatite B/genética
Proteínas do Envelope Viral/genética
Proteínas do Envelope Viral/imunologia
Vacinas contra Hepatite Viral/genética
-Fusão Gênica Artificial
Sequência de Bases
Hepacivirus/genética
Hepacivirus/imunologia
Vírus da Hepatite B/genética
Vírus da Hepatite B/imunologia
Imunização
Técnicas In Vitro
Dados de Sequência Molecular
MICE, INBRED CABDOMENABDOMINAL INJURIESBL
Vacinas de DNA/genética
Limites: Animais
Feminino
Camundongos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-730792
Autor: Santana, Vinicius Canato.
Título: Formulação vacinal trivalente voltada para o controle profilático/terapêutico de tumores induzidos pelo vírus do papiloma humano tipo 16 (HPV-16) e infecções pelos vírus da imunodeficiência adquirida (HIV) e herpes vírus humano (HSV) / Trivalent vaccine formulation focused on the prophylactic / therapeutic control of tumors induced by human papillomavirus type 16 (HPV-16) and infection by human immunodeficiency virus (HIV) and human herpes virus (HSV).
Fonte: São Paulo; s.n; 2014. [76] p. ilus, tab, graf.
Idioma: pt.
Tese: Apresentada a Universidade de São Paulo. Faculdade de Medicina para obtenção do grau de Doutor.
Resumo: As proteínas E7 (HPV), p24 (HIV) e gD (HSV) são exclusivamente expressas por células infectadas ou tumorais e, por isso, são utilizadas como alvos para vacinas com características terapêuticas. Foram desenvolvidas duas vacinas de DNA capazes de expressar as três proteínas virais por meio de um vetor de expressão bicistrônico baseado na sequência IRES. As vacinas, denominadas pIRES I e pIRES II, diferem entre si por transportarem os genes que codificam as proteínas E7 do HPV-16 e p24 do HIV fusionadas à proteína gD do HSV-1 em ordem inversa. Células COS-7 transfectadas com os plasmídeos vacinais expressaram as proteínas alvo, como determinado por imunofluorecência com anticorpos específicos para as proteínas gD, p24 e E7. As vacinas foram testadas em modelo murino quanto à capacidade de gerar anticorpos e células T CD8+ específicas. Observamos que animais vacinados desenvolveram baixas taxas de anticorpos contra gD, p24 e E7. Em contrapartida, demonstramos a indução de células T CD8+ específicas para os três antígenos testados. Os plasmídeos vacinais foram capazes de proteger camundongos inoculados com células tumorais TC-1 (que expressam a proteína E7 do HPV-16), embora apresentando diferentes níveis de proteção em ensaios profiláticos e terapêuticos. As formulações foram testadas em relação à capacidade de proteger animais frente a desafio com o HSV-1 sendo que apenas um deles gerou efeito protetor. Em conclusão, os resultados demonstram que vacinas voltadas para o controle terapêutico de infecções ou processos tumorais associados aos vírus HPV, HIV e HSV representam uma meta viável e promissora

The proteins E7 (HPV), p24 (HIV) and gD (HSV) are exclusively expressed by infected cells or tumors and therefore are used as targets for vaccines with therapeutic characteristics. We developed two DNA vaccines capable of expressing these three viral proteins using a bicistronic expression vector based on IRES sequence. The plasmid vaccines, named pIRES I and pIRES II, differ by carrying the genes that encode proteins of HPV-16 E7 and p24 fused to the HIV protein gD of HSV-1 in reverse order. Transfected COS-7 cells expressed the target proteins, as determined by immunofluorescence with specific antibodies for gD, p24 and E7. The vaccines were tested in mice for their ability to generate antibodies and specific CD8+ T cells. We observed that vaccinated animals developed low levels of antibodies against gD, E7 and p24. In contrast, we demonstrate the induction of specific CD8+ T cells for the three antigens. The plasmid vaccines were able to protect mice inoculated with TC-1 tumor cells (which express the E7 protein of HPV-16), although with different levels of protection in prophylactic and therapeutic trials. The formulations were tested for ability to protect animals against challenge with HSV-1 and only one of them generated a protective effect. In conclusion, the results show that vaccines directed to therapeutic control of infections or tumor process associated with HPV, HSV or HIV represents a promising and viable goal
Descritores: Adjuvantes Imunológicos
Vacinas contra a AIDS
Citocinas
Fator Estimulador de Colônias de Granulócitos e Macrófagos
Herpesvirus Humano 1
HIV-1
Camundongos Endogâmicos BALB C
Vacinas de DNA
Proteínas do Envelope Viral
Limites: Animais
Feminino
Responsável: BR66.1 - Divisão de Biblioteca e Documentação
BR66.1


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Costa, Paulo Inácio da
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Id: lil-723994
Autor: Urbaczek, Ana Carolina; Ribeiro, Lívia Carolina de Abreu; Ximenes, Valdecir Farias; Afonso, Ana; Nogueira, Camila Tita; Generoso, Wesley Cardoso; Alberice, Juliana Vieira; Rudnicki, Martina; Ferrer, Renila; Fonseca, Luiz Marcos da; Costa, Paulo Inácio da.
Título: Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure
Fonte: Mem. Inst. Oswaldo Cruz;109(6):748-756, 09/09/2014. graf.
Idioma: en.
Projeto: FAPESP.
Resumo: The hepatitis C virus (HCV) encodes approximately 10 different structural and non-structural proteins, including the envelope glycoprotein 2 (E2). HCV proteins, especially the envelope proteins, bind to cell receptors and can damage tissues. Endothelial inflammation is the most important determinant of fibrosis progression and, consequently, cirrhosis. The aim of this study was to evaluate and compare the inflammatory response of endothelial cells to two recombinant forms of the HCV E2 protein produced in different expression systems (Escherichia coli and Pichia pastoris). We observed the induction of cell death and the production of nitric oxide, hydrogen peroxide, interleukin-8 and vascular endothelial growth factor A in human umbilical vein endothelial cells (HUVECs) stimulated by the two recombinant E2 proteins. The E2-induced apoptosis of HUVECs was confirmed using the molecular marker PARP. The apoptosis rescue observed when the antioxidant N-acetylcysteine was used suggests that reactive oxygen species are involved in E2-induced apoptosis. We propose that these proteins are involved in the chronic inflammation caused by HCV.
Descritores: Hepacivirus/metabolismo
Células Endoteliais da Veia Umbilical Humana/imunologia
Células Endoteliais da Veia Umbilical Humana/patologia
Óxido Nítrico/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
Proteínas do Envelope Viral/metabolismo
-Apoptose/genética
Arginase/metabolismo
Sobrevivência Celular
Escherichia coli/metabolismo
Fibrose
Expressão Gênica/genética
Engenharia Genética/métodos
Vetores Genéticos/metabolismo
Hepacivirus/imunologia
Antígenos da Hepatite C/metabolismo
Inflamação/metabolismo
/metabolismo
INTERLEUKIN-ABDOMINAL NEOPLASMS/metabolismo
Pichia/metabolismo
Plasmídeos/metabolismo
Proteínas Recombinantes
Fator A de Crescimento do Endotélio Vascular/metabolismo
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-695793
Autor: Ocazionez-Jiménez, Raquel E; Ortiz-Báez, Ayda Susana; Gómez-Rangel, Sergio Yebrail; Miranda-Esquivel, Daniel R.
Título: Virus del dengue de serotipo 1 (DENV-1) de Colombia: su contribución a la presentación del dengue en el departamento de Santander / Dengue virus serotype 1 (DENV-1) from Colombia: its contribution to dengue occurrence in Santander
Fonte: Biomédica (Bogotá);33(supl.1):22-30, set. 2013. graf, tab.
Idioma: es.
Resumo: Introducción. Los cuatro serotipos del virus del dengue circularon en el departamento de Santander entre 1998 y 2008. No existe información sobre el papel del serotipo 1 (DENV-1) en la epidemiología de la enfermedad. Objetivo. Analizar la relación entre el cambio de predominancia del (DENV-1) con su diversificación genética, predominancia de los otros serotipos y presentación del dengue grave. Materiales y métodos. La diversificación genética se estudió por análisis filogenético usando la secuencia del gen E de 12 cepas del virus. Para el análisis se utilizaron datos sobre predominancia de los serotipos obtenidos en estudios previos y datos oficiales de incidencia del dengue. Resultados. Los virus seleccionados se agruparon en el genotipo V junto a (DENV-1) de países de Latinoamérica y se evidenció segregación en cuatro linajes. Los cambios en la predominancia del virus coincidieron con el reemplazo de linaje y esto, a su vez, con incremento en la prevalencia de DENV-2 y DENV-3, e incremento del dengue grave. Conclusión. La diversificación genética podría contribuir a cambios de predominancia de (DENV-1), y la relación del virus con el DENV-2 y DENV-3 en situaciones que favorecen la presentación de casos graves. Se necesitan más estudios para precisar el papel de los serotipos en la epidemiología del dengue.

Introduction: Between 1998 and 2008 all dengue virus serotypes circulated in the Departamento de Santander, an endemic region in northeastern Colombia. No information is available as to the role of serotype 1 (DENV-1) with respect to epidemiology of dengue. Objective: To analyze the relationship between changes in DENV-1 predominance with respect to genetic diversity, prevalence of others serotypes and occurrence of severe dengue. Methods: Virus genetic diversity was studied by phylogenetic analysis comparing E gene sequences from 12 viral strains. Data about serotypes predominance obtained in previous studies and official data about dengue incidence were used for analysis. Results: Selected viruses grouped into genotype V together DENV-1 from Latin America countries, and segregation in four lineages was evidenced. Changes in virus predominance coincided with replacement of lineage, increase in prevalence of DENV-2 and DENV-3 and increase of severe dengue. Conclusion: Genetic divergence could have contributed to changes in DENV-1 predominance. The relationship of the virus with DENV-2 and DENV-3 could create scenarios that promote occurrence of severe cases. More studies are required to ascertain the precise role of serotypes in the epidemiology of dengue.
Descritores: Vírus da Dengue/isolamento & purificação
Dengue/virologia
-Colômbia/epidemiologia
Surtos de Doenças
Vírus da Dengue/classificação
Vírus da Dengue/genética
Vírus da Dengue/patogenicidade
Dengue/epidemiologia
Variação Genética
Genótipo
Incidência
Filogenia
Prevalência
RNA Viral/genética
Alinhamento de Sequência
Homologia de Sequência do Ácido Nucleico
Sorogrupo
Sorotipagem
Dengue Grave/epidemiologia
Dengue Grave/virologia
Virulência
Proteínas do Envelope Viral/genética
Proteínas do Envelope Viral/fisiologia
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Research Support, Non-U.S. Gov't
Responsável: CO332 - Facultad de Medicina



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