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Texto completo SciELO Saúde Pública
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Id: lil-733342
Autor: Ruelas-González, M Guadalupe; Pelcastre-Villafuerte, Blanca Estela; Reyes-Morales, Hortensia.
Título: Maltrato institucional hacia el adulto mayor: percepciones del prestador de servicios de salud y de los ancianos / Institutional abuse toward the elderly: Perceptions of health care providers and older adults
Fonte: Salud pública Méx;56(6):631-637, nov.-dic. 2014.
Idioma: es.
Resumo: Objetivo. Analizar la percepción que el prestador de servicios de salud y el adulto mayor (AM) tienen sobre el maltrato al AM en los servicios públicos de salud, en ciudades seleccionadas de México. Material y métodos. De 2009 a 2012 se realizó un estudio con diseño cualitativo y estrategia de triangulación de fuentes de datos; se efectuaron entrevistas semiestructuradas a 13 prestadores y a 12 ancianos para recuperar su experiencia en el tema. El análisis utilizó procedimientos de la Teoría Fundamentada. Resultados. El maltrato contra el AM es una práctica naturalizada por el personal y por el anciano, la cual se manifiesta de formas diversas. Conclusiones. La institucionalización, profesionalización histórica y falta de conciencia sobre las necesidades de los AM demandan cambios de planeación, organización y supervisión del Sistema de Salud. El personal requiere intervenciones de formación, capacitación y cambio de actitudes/comportamiento, para otorgar atención integral, digna, humana y de respeto a los Derechos Humanos de los AM.

Objective. To analyze the health care providers (HCP) and elderly patients' perceptions about abuse of the elderly by health personnel of public health services, in selected cities in Mexico. Materials and methods. A qualitative study and a strategy of data triangulation were performed during 2009 and 2012; 13 HCPs and 12 elders were interviewed, in order to obtain their experience regarding elder abuse. Grounded Theory proceedings were used for the analysis. Results. Elder abuse is a naturalized practice, from HCP and elderly people's point of view; these perceptions are showed in different ways. Conclusion. Institutionalization, historical professionalization and lack of consciousness about needs of the elderly (sociocultural and economic), require changes in planning, organization and monitoring process in the Health System; training and educational interventions on staff and exchange attitudes and behavior are necessary in order to offer a health care that is comprehensive, decent, human and with respect for the human rights.
Descritores: Antimetabólitos Antineoplásicos/farmacologia
Inibidores Enzimáticos/metabolismo
-Elementos Antissenso (Genética)
Neoplasias da Mama
Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo
Divisão Celular/efeitos dos fármacos
Quinases Ciclina-Dependentes/genética
Quinases Ciclina-Dependentes/metabolismo
Fibroblastos/efeitos dos fármacos
Regulação Neoplásica da Expressão Gênica/fisiologia
Camundongos Knockout
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
RNA Mensageiro/metabolismo
Proteína do Retinoblastoma/metabolismo
Transdução de Sinais/fisiologia
Células Tumorais Cultivadas/citologia
Células Tumorais Cultivadas/efeitos dos fármacos
Células Tumorais Cultivadas/enzimologia
Regulação para Cima/efeitos dos fármacos
Limites: Animais
Responsável: BR1.1 - BIREME

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Id: lil-339344
Autor: Qin, Guangming; Zhang, Yongxue; Hnatowich, D. J; An, Rui; Gao, Zairong; Cao, Wei; Cao, Guoxiang.
Título: Technetium-99m labeled antisense oligonucleotide-noninvasive tumor imaging in mice
Fonte: Rev. med. nucl. Alasbimn j;5(19), ene. 2003.
Idioma: en.
Resumo: The 15-mer oligonucleotide sequence was synthesized, aminolinked (sense and antisense phosphodiester) and conjugated with S-Acetyl-NHS-MAG3 by a N-hydroxy-succinimide derivative. The purified MAG3-DNA was radiolabeled with 99mTc by transchelation from sodium tartrate and free 99mTc was separated by gel filtration. The radiolabeled antisense and sense probes were injected intravenously in mammary tumor-bearing KM mice(1×106 cells,6 days post inoculation).Biodistribution was studied and the mice were imaged.Essentially complete conjugation was achieved by reverse-phase Sep-Pak C18 chromatography analysis.The MAG3-DNA was labeled with 99mTc at room temperature and neutral pH, with a mean labeling efficiency of 80.11 percent (s.d=2.96 percent , N=4). After labeling, the stability of the DNA in saline or serum was retained as determined by reverse-phase Sep-Pak C18 chromatography analysis, except a shift at 30 min in serum incubation which might suggest a short time serum protein binding. The labeled antisense DNA still remained the ability to hybridize with its complementary DNA. The pharmacodynamics of 99mTc labeled c-myc probes (antisense and sense) in mammary tumor-bearing KM mice did not change with the time postinjection. The highest accumulation of label was in the liver first, with the kidney and small bowel next. The injected activity localized in the lesion as early as 30min and reached a saturation value at 4hr. The accumulation of radioactivity in the tumor was lower at all time points in animals receiving the blocking oligonucleotides or sense probes. All images obtained with 99mTc-MAG3-c-myc antisense probes showed specific accumulation of radioactivity at the site of tumor. Radiolabel rapidly accumulates at the site of tumor and remains associated with the site even though circulation levels of radioactivity have greatly diminished. The tumor was readily evident since 45min and reached the highest tumor-to-muscle ratio at 4hr. The quite encouraging result was obtained at 20hr to 22hr when the background activity was diminished sufficiently. Positive imaging was not obtained in case of control group (in which non-conjugated, non-labeled antisense oligonucleotides were administered 2hr before the radiolabeled antisense probes were injected) and of sense group. Conclusion The 99mTc labeled antisense probe may provide a sensible and specific tool for noninvasive imaging of c-myc oncogene mRNA for a variety of malignant tumors at an earlier stage
Descritores: Análise de Sequência com Séries de Oligonucleotídeos/métodos
Neoplasias Mamárias Experimentais
-Elementos Antissenso (Genética)/análise
Genes myc
Limites: Animais
Responsável: CL1.1 - Biblioteca Central

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Texto completo SciELO Brasil
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Id: lil-234893
Autor: Flatschart, R. B; Sogayar, M. C.
Título: Functional analysis of newly discovered growth control genes: experimental approaches
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;32(7):867-75, July 1999.
Idioma: en.
Conferência: Apresentado em: International Symposium on \"Signal Transduction and Gene Expression in Cell Proliferation and Differentiation\", 1, Säo Paulo, Aug. 31-Sept. 2, 1998.
Resumo: A large number of DNA sequences corresponding to human and animal transcripts have been filed in data banks, as cDNAs or ESTs (expression sequence tags). However, the actual function of their corresponding gene products is still largely unknown. Several of these genes may play a role in regulation of important biological processes such as cell division, differentiation, malignant transformation and oncogenesis. Elucidation of gene function is based on 2 main approaches, namely, overexpression and expression interference, which respectively mimick or suppress a given phenotype. The currently available tools and experimental approaches to gene functional analysis and the most recent advances in mass cDNA screening by functional analysis are discussed
Descritores: Elementos Antissenso (Genética)
Expressão Gênica
RNA Catalítico
-DNA Recombinante
Limites: Animais
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME

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