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Pesquisa : D13.150.480.645 [Categoria DeCS]
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Id: lil-561220
Autor: Santos, A. O; Pereira, J. P; Pedroso de Lima, M. C; Simões, S; Moreira, J. N.
Título: In vitro modulation of Bcl-2 levels in small cell lung cancer cells: effects on cell viability
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;43(10):1001-1009, Oct. 2010. ilus, tab.
Idioma: en.
Resumo: Small cell lung cancer (SCLC) is an aggressive disease, representing 15 percent of all cases of lung cancer, has high metastatic potential and low prognosis that urgently demands the development of novel therapeutic approaches. One of the proposed approaches has been the down-regulation of BCL2, with poorly clarified and controversial therapeutic value regarding SCLC. The use of anti-BCL2 small interfering RNA (siRNA) in SCLC has never been reported. The aim of the present study was to select and test the in vitro efficacy of anti-BCL2 siRNA sequences against the protein and mRNA levels of SCLC cells, and their effects on cytotoxicity and chemosensitization. Two anti-BCL2 siRNAs and the anti-BCL2 G3139 oligodeoxynucleotide (ODN) were evaluated in SCLC cells by the simultaneous determination of Bcl-2 and viability using a flow cytometry method recently developed by us in addition to Western blot, real-time reverse-transcription PCR, and cell growth after single and combined treatment with cisplatin. In contrast to previous reports about the use of ODN, a heterogeneous and up to 80 percent sequence-specific Bcl-2 protein knockdown was observed in the SW2, H2171 and H69 SCLC cell lines, although without significant sequence-specific reduction of cell viability, cell growth, or sensitization to cisplatin. Our results question previous data generated with antisense ODN and supporting the present concept of the therapeutic interest in BCL2 silencing per se in SCLC, and support the growing notion of the necessity of a multitargeting molecular approach for the treatment of cancer.
Descritores: Neoplasias Pulmonares/tratamento farmacológico
Oligorribonucleotídeos Antissenso/farmacologia
/metabolismo
PROTO-ONCOGENE PROTEINS C-BCL-TEMEFOS/metabolismo
RNA Interferente Pequeno/farmacologia
Carcinoma de Pequenas Células do Pulmão/tratamento farmacológico
-Antineoplásicos/farmacologia
Western Blotting
Sobrevivência Celular/efeitos dos fármacos
Cisplatino/farmacologia
Regulação para Baixo
Citometria de Fluxo
Inativação Gênica
Neoplasias Pulmonares/metabolismo
/efeitos dos fármacos
PROTO-ONCOGENE PROTEINS C-BCL-TEMEFOS/efeitos dos fármacos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Carcinoma de Pequenas Células do Pulmão/metabolismo
Células Tumorais Cultivadas
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo de Validação
Responsável: BR1.1 - BIREME


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Id: lil-490400
Autor: Orrego, Patricio.
Título: ¿Los ARNs antisentido cumplen algún rol en eucariontes? / Does antisense RNA have any role in eukaryotes ?
Fonte: Rev. cienc. salud;10(1):34-41, dic. 2006. ilus.
Idioma: es.
Resumo: The presence of antisense transcripts has been described in a wide variety of eucaryotic organisms, with a diversity of implied biological functions in development, control of cell cycles, hormonal responses, control of proliferation, structure, viral replication, and others. Reports have suggested that this type of control may operate at different levels of genic expression, whether in transcription, maturation, transport, stability, or translation of a given trascript. It is evident that the expression of antisense RNA has true importance in Eukaryotes, as already established in prokaryotic organisms. The objective of the present review is to present the advances in the mechanics of regulation, and roles already established for the expressions of antisense RNA in eukaryotic organisms.

La presencia de transcritos antisentidos ha sido descrita en una amplia variedad de organismos eucariontes, implicados en una diversidad de funciones biológicas como el desarrollo, el control del ciclo celular, respuesta hormonal, control de la proliferación, estructura, replicación viral, etc. Los informes indican que este control puede darse a diversos niveles de la expresión génica, ya sea en la transcripción, maduración, transporte, estabilidad y traducción de un determinado transcrito. Es evidente que la expresión del ARN antisentido tiene una real importancia en eucariontes, como ya ha sido establecido en organismos procariontes. Esta revisión tiene como objetivo mostrar los avances en los mecanismos de regulación y roles ya establecidos de la expresión de ARN antisentidos en organismos eucariontes.
Descritores: Fatores de Iniciação em Eucariotos/genética
Oligorribonucleotídeos Antissenso
RNA
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central



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