Base de dados : LILACS
Pesquisa : D13.444.154 [Categoria DeCS]
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Id: biblio-951907
Autor: Oliveira, Isadora Bernardo David de; Hirata, Rosario Dominguez Crespo.
Título: Circulating cell-free DNA as a biomarker in the diagnosis and prognosis of colorectal cancer
Fonte: Braz. J. Pharm. Sci. (Online);54(1):e17368, 2018. tab, graf.
Idioma: en.
Resumo: Abstract Colorectal cancer (CRC) is a disease without evident clinical symptoms in early stages, leading to late diagnosis and disease management. Current diagnostic and prognostic tools require invasive procedures and circulating molecular biomarkers fail to have optimal sensitivity and specificity. Circulating biomarkers with high clinical performance may be valuable for early diagnosis and prognosis of CRC. The purpose of this review was to investigate the application of circulating cell-free DNA (ccfDNA) in CRC diagnosis and prognosis and the analytical methods used in blood samples in articles published between 2005 and 2016. Based on specific inclusion and exclusion criteria, 26 articles were selected. Most studies used ccfDNA quantification as the molecular biomarker. The analytical method was mainly based on the quantitative polymerase chain reaction (qPCR). Biomarkers based on aberrantly methylated genes (n=6) and ccfDNA integrity/fragmentation (n=2) were also used for the CRC diagnosis. The CRC prognosis used the detection of oncogene mutations, such as KRAS and BRAF, in ccfDNA. Significant differences were found in variables among the studies revealing potential bias. ccfDNA quantification as a diagnostic biomarker for CRC has promising results but it lacks clinical specificity since other diseases present a similar increase in ccfDNA content. However, increasing research in the epigenomic field can lead the way to a clinically specific biomarker for the CRC early diagnosis. As for the analytical method, qPCR and derivatives seem to be a perfectly valid technique. The use of ccfDNA quantification in CRC prognosis seems promising. The attempt to use the ccfDNA quantification in clinical practice may reside in the prognosis using a qPCR technique.
Descritores: Prognóstico
Neoplasias Colorretais/diagnóstico
Ácidos Nucleicos Livres/efeitos adversos
-Biomarcadores
Diagnóstico Precoce
Células Neoplásicas Circulantes
Tipo de Publ: Revisão
Responsável: BR40.1 - DBD - Divisão de Biblioteca e Documentacão do Conjunto das Químicas


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Id: lil-737130
Autor: Musci, Thomas J.
Título: Cell-free DNA: a new era in aneuploidy testing
Fonte: Femina;42(4):161-164, jul-ago. 2014.
Idioma: pt.
Descritores: Análise Citogenética/métodos
Ácidos Nucleicos Livres/análise
Aneuploidia
-Cuidado Pré-Natal
Fatores de Risco
Síndrome de Down/embriologia
Testes para Triagem do Soro Materno/métodos
Limites: Feminino
Gravidez
Tipo de Publ: Editorial
Responsável: BR1365.1 - Biblioteca Biomédica A - CB/A


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Id: lil-749122
Autor: Fonseca, Eduardo Borges da; Cruz, Jader; Sá, Renato de Augusto; Di Renzo, Gian Carlo; Nicolaides, Kypros.
Título: Rastreamento de aneuploidias no primeiro trimestre de gestação: evolução da idade materna à avaliação do DNA fetal livre no sangue materno / Screening for aneuploidies in the first trimester of pregnancy: evolution from maternal age to cell-free DNA testing in maternal blood
Fonte: Femina;42(2):87-93, mar-abr. 2014. tab, ilus.
Idioma: pt.
Resumo: O rastreamento fetal de aneuploidia apresentou uma evolução fantástica a partir da avaliação individual da idade materna até os dias atuais, na qual evidências sugerem que o teste de avaliação do DNA fetal livre no sangue materno detecta mais de 99% dos casos de trissomia do cromossomo 21 e, aproximadamente, 98% dos casos de trissomia do 18 e 92%, do 13, com taxas de falso-positivo de 0,1; 0,1 e 0,3%, respectivamente. Recentemente, o grupo de trabalho em boas práticas médicas da Federação Internacional de Ginecologia e Obstetrícia recomendou que todas as gestantes, independentemente da idade, deveriam realizar uma avaliação de risco para aneuploidias por meio da translucência nucal, do teste combinado ou do teste de DNA fetal livre no sangue materno. O teste invasivo para diagnóstico de aneuploidia não deveria ser realizado considerando apenas a idade materna como fator de risco. O objetivo desta revisão foi apresentar esta nova ferramenta de rastreio, presente em muitos centros, e descrever as estratégias para implementação de tal tecnologia na prática clínica diária.(AU)

Screening for fetal aneuploidy has a tremendous evolution from maternal age to now where recent evidence suggests that cell-free DNA testing in maternal blood can detect more than 99% of cases of trisomy 21, about 98% of trisomy 18, and 92% of trisomy 13, with respective false-positive rates of 0.1, 0.1, and 0.3%. Recently, the working group on the best practice on maternal fetal medicine of the International Federation of Gynecology and Obstetrics has recommended as a good medical practice that pregnant women, regardless of maternal age, be offered prenatal assessment for aneuploidy through nuchal translucency, combined test, or cell-free DNA testing. The invasive procedure for diagnosis of aneuploidy should be avoided taking into account only the maternal age as a risk factor nowadays. The purpose of this review was to present this new screening tool available in most centers and to describe the strategies for implementation of this technology on the daily clinical practice.(AU)
Descritores: Primeiro Trimestre da Gravidez
Testes para Triagem do Soro Materno/métodos
Ácidos Nucleicos Livres/química
Aneuploidia
-Cuidado Pré-Natal/métodos
Fatores de Risco
Limites: Seres Humanos
Feminino
Gravidez
Responsável: BR1.1 - BIREME


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Id: biblio-910469
Autor: Abraham, J; Singh, S; Joshi, S.
Título: Liquid biopsy: emergence of a new era in personalized cancer care
Fonte: Appl. cancer res;38:1-17, jan. 30, 2018. tab, ilus.
Idioma: en.
Resumo: The most successful treatment for cancer involves identifying druggable, biological markers for targeted therapy. In the clinical setting, surgical removal of tumors is the only procedure for identifying such targetable molecules. Shed from tumor cells, these markers are also present in circulating blood, albeit in very negligible amounts. Liquid biopsy is a procedure performed on a blood sample to look for such circulating cancer markers cells or pieces of nucleic acid from the tumor. The procedure shows promise in revolutionizing personalized cancer treatments. Here we briefly review the technique, characterization, and its utilization in clinics
Descritores: Biomarcadores
Ácidos Nucleicos Livres
Biópsia Líquida
Terapia de Alvo Molecular
Células Neoplásicas Circulantes
Limites: Seres Humanos
Tipo de Publ: Revisão
Responsável: BR30.1 - Biblioteca



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