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Id: biblio-1145264
Autor: Routray, Samapika; Rath, Shakti; Mohanty, Neeta.
Título: Prevalence of methicillin resistant staphylococcus aureus isolated from saliva samples of patients with oral squamous cell carcinoma / revalencia de Staphylococcus aureus resistente a la meticilina en muestras de saliva de pacientes con carcinoma oral de células escamosas
Fonte: J. oral res. (Impresa);8(1):30-36, feb. 28, 2019. ilus, tab.
Idioma: en.
Resumo: Objectives: To study the prevalence of methicillin resistant Staphylococcus aureus (MRSA) in saliva samples of pre-surgical oral squamous cell carcinoma (OSCC) patients along with their resistance pattern to other antibiotics. Methods: Saliva samples of OSCC patients were collected and processed for isolation of MRSA. Staphylococcus aureus isolates were primarily identified using standard microbiological methods like biochemical assays, specialized media and latex agglutination test. Confirmation of MRSA strains was done by growing the isolates on MRSA agar and by using PCR to amplify two MRSA specific genes. All the isolated Staphylococcus aureus strains were subjected to antibiotic sensitivity tests. Results: A total of 17 Staphylococcus aureus strains were isolated from 50 saliva samples of pre-surgical OSCC patients of which 13 were confirmed to be MRSA. These MRSA strains were also found to be mostly resistant to other commonly used antibiotics. Univariate analysis revealed that most patients with MRSA infections had a prior history of hospitalization and surgery. Also, it was confirmed that patients with other comorbidities and infections were more prone to having MRSA present in the saliva. Conclusion: The majority of Staphylococcus aureus isolates from the saliva of OSCC patients were MRSA, and were resistant to several other commonly used antibiotics.

Objetivos: Estudiar la prevalencia de Staphylococcus aureus resistente a la meticilina (MRSA) en muestras de saliva prequirúrgicas de pacientes con carcinoma oral de células escamosas (COCE) junto con su patrón de resistencia a otros antibióticos. Métodos: Se recolectaron muestras de saliva de pacientes con COCE y se procesaron para el aislamiento de SARM. Los aislamientos de Staphylococcus aureus se identificaron principalmente mediante métodos microbiológicos estándar, como los análisis bioquímicos, los medios especializados y la prueba de aglutinación con látex. La confirmación de las cepas de SARM se realizó cultivando los aislados en agar SARM y utilizando PCR para amplificar dos genes específicos de SARM. Todas las cepas aisladas de Staphylococcus aureus se sometieron a pruebas de sensibilidad a los antibióticos. Resultados: Se aislaron un total de 17 cepas de Staphylococcus aureus a partir de 50 muestras de saliva de pacientes prequirúrgicos con COCE, de los cuales solo se confirmó que 13 eran SARM. También se encontró que estas cepas de SARM son resistentes a otros antibióticos de uso común. El análisis univariado reveló que la mayoría de los pacientes con infecciones por SARM tenían antecedentes previos de hospitalización y cirugía. Además, se confirmó que los pacientes con otras comorbilidades e infecciones eran más propensos a las infecciones por SARM. Conclusión: la mayoría de los aislamientos de Staphylococcus aureusde la saliva de los pacientes con OSCC fueron MRSA y fueron resistentes a varios otros antibióticos de uso común.
Descritores: Staphylococcus/isolamento & purificação
Resistência Microbiana a Medicamentos
Staphylococcus aureus Resistente à Meticilina/genética
Carcinoma de Células Escamosas de Cabeça e Pescoço
Boca/microbiologia
-Saliva
DNA Bacteriano/genética
Prevalência
Antibacterianos
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: CL30.1 - Biblioteca


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Id: biblio-1132581
Autor: Damghani, Mohammad Ali; Dehghan, Elham.
Título: Is there any association between Helicobacter pylori and otitis media with effusion? / Há associação entre Helicobacter pylori e otite média com efusão?
Fonte: Braz. j. otorhinolaryngol. (Impr.);86(2):217-221, March-Apr. 2020. tab, graf.
Idioma: en.
Resumo: Abstract Introduction: It is proposed that Helicobacter pylori can be responsible for the development of otitis media with effusion. Objective: The aim of this study is to investigate the prevalence of H. pylori in the adenoid tissue and fluid of the middle ear in patients who suffer from adenoid hyperplasia and otitis media with effusion in comparison with those who suffer from adenoid hyperplasia without otitis media with effusion. Methods: This is a case-control study that was carried out in 50 children of age 2-7 years old who were admitted with adenoid hyperplasia. Patients were divided into case and control groups. The study group included 25 patients with adenoid hyperplasia and otitis media with effusion and the control group included 25 patients with adenoid hyperplasia without otitis media with effusion. The patients in both groups underwent surgical adenoidectomy. For the case group we carried out myringotomy and placement of tympanostomy tube, and fluid samples were collected under sterile conditions. The samples were sent to the laboratory for polymerase chain reactions. Results: In the case group H. pylori was found to be positive in 18 samples of the middle ear fluid (70%) and in 1 polymerase chain reaction adenoid tissue sample (4%). In the control group H. pylori was positive in 3 samples of adenoid tissues (12%). There was no gender difference. Conclusion: H. pylori is one of the important bacteria that plays a role in the pathogenesis of otitis media with effusion. Whether adenoid tissue may be a reservoir for H. Pylori is unclear.

Resumo Introdução: Propõe-se que o Helicobacter pylori possa ser responsável pelo desenvolvimento de otite média com efusão. Objetivo: Investigar a prevalência de H. pylori no tecido adenoideano e no fluido da orelha média em pacientes com hiperplasia de adenoide e otite média com efusão em comparação àqueles com hiperplasia de adenoide sem otite média com efusão. Método: Este é um estudo de caso-controle feito em 50 crianças de 2 a 7 anos, com sinais e sintomas de hiperplasia de adenoide. Os pacientes foram divididos em grupo de estudo e grupo controle. O grupo de estudo incluiu 25 pacientes com hiperplasia de adenoide e otite média com efusão e o grupo controle incluiu 25 pacientes com hiperplasia de adenoide sem otite média com efusão. Os pacientes dos dois grupos foram submetidos a adenoidectomia e, no grupo de estudo, realizou-se também miringotomia com colocação de tubo de ventilação e amostras de fluidos foram coletadas sob condições estéreis. As amostras foram enviadas para o laboratório, para investigação por reação de polimerase em cadeia. Resultados: No grupo de estudo, houve positividade para H. pylori em 18 amostras do fluido de orelha média (70%) e uma amostra de tecido adenoideano foi positiva na reação de polimerase em cadeia (4%). No grupo controle, houve positividade para H. pylori em 3 amostras de tecido adenoideano (12%). Não houve diferença entre os gêneros. Conclusão: H. pylori é uma das bactérias importantes que desempenham um papel na patogênese da otite médica com efusão. Se o tecido adenoideano pode ou não representar um reservatório para H. pylori ainda necessita ser esclarecido.
Descritores: Otite Média com Derrame/microbiologia
DNA Bacteriano/genética
Helicobacter pylori/genética
Infecções por Helicobacter/diagnóstico
-Estudos de Casos e Controles
Reação em Cadeia da Polimerase
Helicobacter pylori/isolamento & purificação
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Responsável: BR1.1 - BIREME


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Id: lil-780805
Autor: Berber, Ismet; Avsar, Cumhur; Yegin, Zeynep; Tekerci, Melike; Civek, Seyhan.
Título: Molecular epidemiology of Pseudomonas aeruginosa clinical isolates
Fonte: Braz. j. infect. dis;20(2):224-225, Mar.-Apr. 2016. graf.
Idioma: en.
Descritores: Pseudomonas aeruginosa/genética
Fatores de Virulência/genética
Antibacterianos/farmacologia
-Pseudomonas aeruginosa/efeitos dos fármacos
Turquia
DNA Bacteriano/análise
Epidemiologia Molecular
Genes Bacterianos/genética
Limites: Humanos
Tipo de Publ: Carta
Responsável: BR1.1 - BIREME


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Id: lil-780813
Autor: Café Oliveira, Luita Nice; Muniz-Sobrinho, Jairo da Silva; Viana-Magno, Luiz Alexandre; Oliveira Melo, Sônia Cristina; Macho, Antonio; Rios-Santos, Fabrício.
Título: Detection of multidrug-resistant Mycobacterium tuberculosis strains isolated in Brazil using a multimarker genetic assay for katG and rpoB genes
Fonte: Braz. j. infect. dis;20(2):166-172, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Resumo: Abstract Multidrug-resistant tuberculosis (MDRTB) is a serious world health problem that limits public actions to control tuberculosis, because the most used anti-tuberculosis first-line drugs fail to stop mycobacterium spread. Consequently, a quick detection through molecular diagnosis is essential to reduce morbidity and medical costs. Despite the availability of several molecular-based commercial-kits to diagnose multidrug-resistant tuberculosis, their diagnostic value might diverge worldwide since Mycobacterium tuberculosis genetic variability differs according to geographic location. Here, we studied the predictive value of four common mycobacterial mutations in strains isolated from endemic areas of Brazil. Mutations were found at the frequency of 41.9% for katG, 25.6% for inhA, and 69.8% for rpoB genes in multidrug-resistant strains. Multimarker analysis revealed that combination of only two mutations (“katG/S315T + rpoB/S531L”) was a better surrogate of multidrug-resistant tuberculosis than single-marker analysis (86% sensitivity vs. 62.8%). Prediction of multidrug-resistant tuberculosis was not improved by adding a third or fourth mutation in the model. Therefore, rather than using diagnostic kits detecting several mutations, we propose a simple dual-marker panel to detect multidrug-resistant tuberculosis, with 86% sensitivity and 100% specificity. In conclusion, this approach (previous genetic study + analysis of only prevalent markers) would considerably decrease the processing costs while retaining diagnostic accuracy.
Descritores: Proteínas de Bactérias/genética
RNA Polimerases Dirigidas por DNA/genética
Catalase/genética
Farmacorresistência Bacteriana Múltipla/genética
Isoniazida/farmacologia
Antituberculosos/farmacologia
-Rifampina/farmacologia
DNA Bacteriano
Testes de Sensibilidade Microbiana
Marcadores Genéticos
Reação em Cadeia da Polimerase
Valor Preditivo dos Testes
Sensibilidade e Especificidade
Tuberculose Resistente a Múltiplos Medicamentos/microbiologia
Genótipo
Mutação/genética
Mycobacterium tuberculosis/efeitos dos fármacos
Mycobacterium tuberculosis/genética
Limites: Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo de Validação
Responsável: BR1.1 - BIREME


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Id: lil-789480
Autor: Raveendran, Reena; Wattal, Chand.
Título: Utility of multiplex real-time PCR in the diagnosis of extrapulmonary tuberculosis
Fonte: Braz. j. infect. dis;20(3):235-241, May.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Objective The diagnosis of extrapulmonary tuberculosis is still a challenge because of its pauci-bacillary nature. The aim of the study was to evaluate the role of a multiplex PCR assay in the diagnosis of extrapulmonary tuberculosis and to compare the efficiency of two targets, IS6110 and MPB64 to detect Mycobacterium tuberculosis. Methods 150 extrapulmonary samples (61 pus/aspirate, 46 tissue, 32 body fluids, and 11 urine) from clinically suspected cases of tuberculosis were included in the study. All the samples were subjected to direct fluorescent microscopy, TB culture (BacT/ALERT 3D, biomerieux, Durham, North Carolina, USA) and a Multiplexed Tandem PCR targeting two mycobacterial DNA sequences, IS6110 and MPB64. Master-Mix reagents and primers were prepared by AusDiagnostics Pvt. Ltd (Alexandria, New South Wales, Australia). The performance of the assay was assessed using a composite gold standard, which included clinical characteristics, microbiology smear as well as culture, histopathology, cytology, radiology, and response to antitubercular therapy. Results 20.3%, 23.6%, and 45.3% of specimens were positive by smear, culture, and PCR, respectively. The sensitivity and specificity of the multiplex PCR was 91.9% and 88.4%, respectively, using the composite gold standard. Positive and negative predictive values of the PCR were estimated as 85.1% and 93.8%, respectively. Higher positivity was observed with target IS6110 (44.6%) as compared to target MPB64 (18.9%). The sensitivities of IS6110 and MPB64 individual targets were 90.3% and 64.5%, respectively, and specificities were 88.4% and 97.7%, respectively. Conclusion PCR can play an important role in rapid and accurate diagnosis of extrapulmonary tuberculosis. IS6110 alone is an effective target in our part of the country.
Descritores: Tuberculose/diagnóstico
Reação em Cadeia da Polimerase Multiplex
Mycobacterium tuberculosis/genética
Antígenos de Bactérias/genética
-DNA Bacteriano/análise
Amplificação de Genes
Técnicas Bacteriológicas/métodos
Sensibilidade e Especificidade
Meios de Cultura
Limites: Humanos
Responsável: BR1.1 - BIREME


  6 / 598 LILACS  
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Id: biblio-828119
Autor: de Filippis, Ivano; Andrade, Claudia Ferreira de; Caldeira, Nathalia; Azevedo, Aline Carvalho de; Almeida, Antonio Eugenio de.
Título: Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples
Fonte: Braz. j. infect. dis;20(4):335-341, July-Aug. 2016. tab, graf.
Idioma: en.
Projeto: INCQS/FIOCRUZ; . FAPERJ.
Resumo: Abstract Background Several in-house PCR-based assays have been described for the detection of bacterial meningitis caused by Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae from clinical samples. PCR-based methods targeting different bacterial genes are frequently used by different laboratories worldwide, but no standard method has ever been established. The aim of our study was to compare different in-house and a commercial PCR-based tests for the detection of bacterial pathogens causing meningitis and invasive disease in humans. Methods A total of 110 isolates and 134 clinical samples (99 cerebrospinal fluid and 35 blood samples) collected from suspected cases of invasive disease were analyzed. Specific sets of primers frequently used for PCR-diagnosis of the three pathogens were used and compared with the results achieved using the multiplex approach described here. Several different gene targets were used for each microorganism, namely ctrA, crgA and nspA for N. meningitidis, ply for S. pneumoniae, P6 and bexA for H. influenzae. Results All used methods were fast, specific and sensitive, while some of the targets used for the in-house PCR assay detected lower concentrations of genomic DNA than the commercial method. An additional PCR reaction is described for the differentiation of capsulated and non-capsulated H. influenzae strains, the while commercial method only detects capsulated strains. Conclusions The in-house PCR methods here compared showed to be rapid, sensitive, highly specific, and cheaper than commercial methods. The in-house PCR methods could be easily adopted by public laboratories of developing countries for diagnostic purposes. The best results were achieved using primers targeting the genes nspA, ply, and P6 which were able to detect the lowest DNA concentrations for each specific target.
Descritores: Haemophilus influenzae/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Meningite por Haemophilus/diagnóstico
Meningite Meningocócica/diagnóstico
Meningite Pneumocócica/diagnóstico
Neisseria meningitidis/isolamento & purificação
-Streptococcus pneumoniae/isolamento & purificação
Streptococcus pneumoniae/genética
DNA Bacteriano/genética
Haemophilus influenzae/genética
Sensibilidade e Especificidade
Primers do DNA
Meningite por Haemophilus/microbiologia
Meningite Meningocócica/microbiologia
Meningite Pneumocócica/microbiologia
Neisseria meningitidis/genética
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


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Id: biblio-828136
Autor: Martínez-Meléndez, Adrián; Morfín-Otero, Rayo; Villarreal-Treviño, Licet; Camacho-Ortíz, Adrián; González-González, Gloria; Llaca-Díaz, Jorge; Rodríguez-Noriega, Eduardo; Garza-González, Elvira.
Título: Molecular epidemiology of coagulase-negative bloodstream isolates: detection of Staphylococcus epidermidis ST2, ST7 and linezolid-resistant ST23
Fonte: Braz. j. infect. dis;20(5):419-428, Sept.-Oct. 2016. tab, graf.
Idioma: en.
Projeto: CONACyT (Mexican Council for Science and Technology).
Resumo: Abstract The mechanisms contributing to persistence of coagulase-negative staphylococci are diverse; to better understanding of their dynamics, the characterization of nosocomial isolates is needed. Our aim was to characterize phenotypic and molecular characteristics of Staphylococcus epidermidis and Staphylococcus haemolyticus human blood isolates from two tertiary care hospitals in Mexico, the Hospital Universitario in Monterrey and the Hospital Civil in Guadalajara. Antimicrobial susceptibility was determined. Biofilm formation was assessed by crystal violet staining. Detection of the ica operon and Staphylococcal Cassette Chromosome mec typing were performed by PCR. Clonal relatedness was determined by Pulsed-fiel gel electrophoresis and Multi locus sequence typing. Methicillin-resistance was 85.5% and 93.2% for S. epidermidis and S. haemolyticus, respectively. Both species showed resistance >70% to norfloxacin, clindamycin, levofloxacin, trimethoprim/sulfamethoxazole, and erythromycin. Three S. epidermidis and two S. haemolyticus isolates were linezolid-resistant (one isolate of each species was cfr+). Most isolates of both species were strong biofilm producers (92.8% of S. epidermidis and 72.9% of S. haemolyticus). The ica operon was amplified in 36 (43.4%) S. epidermidis isolates. SCCmec type IV was found in 47.2% of the S. epidermidis isolates and SCCmec type V in 14.5% of S. haemolyticus isolates. No clonal relatedness was found in either species. Resistance to clindamycin, levofloxacin, erythromycin, oxacillin, and cefoxitin was associated with biofilm production for both species (p < 0.05). A G2576T mutation in 23S rRNA gene was detected in an S. haemolyticus linezolid-resistant isolate. All linezolid-resistant S. epidermidis isolates belonged to ST23; isolate with SCCmec type IV belonged to ST7, and isolate with SCCmec type III belonged to ST2. This is the first report of ST7 in Mexico. There was a high genetic diversity in both species, though both species shared characteristics that may contibute to virulence.
Descritores: Staphylococcus epidermidis/isolamento & purificação
Staphylococcus epidermidis/efeitos dos fármacos
Coagulase/sangue
Staphylococcus haemolyticus/efeitos dos fármacos
Linezolida/farmacologia
Antibacterianos/farmacologia
-Valores de Referência
Staphylococcus epidermidis/genética
DNA Bacteriano
Testes de Sensibilidade Microbiana
Eletroforese em Gel de Campo Pulsado
Coagulase/isolamento & purificação
Coagulase/genética
Biofilmes/crescimento & desenvolvimento
Biofilmes/efeitos dos fármacos
Farmacorresistência Bacteriana
Staphylococcus haemolyticus/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Tipagem de Sequências Multilocus
Reação em Cadeia da Polimerase Multiplex
México
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo Multicêntrico
Responsável: BR1.1 - BIREME


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Id: biblio-828162
Autor: Zurita, Jeannete; Barba, Pedro; Ortega-Paredes, David; Mora, Marcelo; Rivadeneira, Sebastián.
Título: Local circulating clones of Staphylococcus aureus in Ecuador
Fonte: Braz. j. infect. dis;20(6):525-533, Nov.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT The spread of pandemic Staphylococcus aureus clones, mainly methicillin-resistant S. aureus (MRSA), must be kept under surveillance to assemble an accurate, local epidemiological analysis. In Ecuador, the prevalence of the USA300 Latin American variant clone (USA300-LV) is well known; however, there is little information about other circulating clones. The aim of this work was to identify the sequence types (ST) using a Multiple-Locus Variable number tandem repeat Analysis 14-locus genotyping approach. We analyzed 132 S. aureus strains that were recovered from 2005 to 2013 and isolated in several clinical settings in Quito, Ecuador. MRSA isolates composed 46.97% (62/132) of the study population. Within MRSA, 37 isolates were related to the USA300-LV clone (ST8-MRSA-IV, Panton-Valentine Leukocidin [PVL] +) and 10 were related to the Brazilian clone (ST239-MRSA-III, PVL−). Additionally, two isolates (ST5-MRSA-II, PVL−) were related to the New York/Japan clone. One isolate was related to the Pediatric clone (ST5-MRSA-IV, PVL−), one isolate (ST45-MRSA-II, PVL−) was related to the USA600 clone, and one (ST22-MRSA-IV, PVL−) was related to the epidemic UK-EMRSA-15 clone. Moreover, the most prevalent MSSA sequence types were ST8 (11 isolates), ST45 (8 isolates), ST30 (8 isolates), ST5 (7 isolates) and ST22 (6 isolates). Additionally, we found one isolate that was related to the livestock associated S. aureus clone ST398. We conclude that in addition to the high prevalence of clone LV-ST8-MRSA-IV, other epidemic clones are circulating in Quito, such as the Brazilian, Pediatric and New York/Japan clones. The USA600 and UK-EMRSA-15 clones, which were not previously described in Ecuador, were also found. Moreover, we found evidence of the presence of the livestock associated clone ST398 in a hospital environment.
Descritores: Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/genética
Toxinas Bacterianas/genética
Exotoxinas/genética
Leucocidinas/genética
Antibacterianos/farmacologia
-Staphylococcus aureus/isolamento & purificação
Staphylococcus aureus/classificação
DNA Bacteriano
Testes de Sensibilidade Microbiana
Prevalência
Fatores de Virulência/genética
Equador
Staphylococcus aureus Resistente à Meticilina/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/classificação
Staphylococcus aureus Resistente à Meticilina/genética
Tipagem de Sequências Multilocus
Genótipo
Limites: Humanos
Masculino
Feminino
Recém-Nascido
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-839184
Autor: Campana, Eloiza Helena; Xavier, Danilo Elias; Petrolini, Fernanda Villas-Boas; Cordeiro-Moura, Jhonatha Rodrigo; Araujo, Maria Rita Elmor de; Gales, Ana Cristina.
Título: Carbapenem-resistant and cephalosporin-susceptible: a worrisome phenotype among Pseudomonas aeruginosa clinical isolates in Brazil
Fonte: Braz. j. infect. dis;21(1):57-62, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed by PFGE. Carbapenems hydrolysis was not detected by carbapenemase assay in the carbapenem-resistant and cephalosporin-susceptible P. aueruginosa clinical isolates. OprD decreased expression was observed in all P. aeruginosa isolates by qRT-PCR. The outer membrane protein profile by SDS-PAGE suggested a change in the expression of the 46 kDa porin that could correspond to OprD porin. The isolates were clustered into 17 genotypes without predominance of a specific PFGE pattern. These results emphasize the involvement of multiple chromosomal mechanisms in carbapenem-resistance among clinical isolates of P. aeruginosa, alert for adaptation of P. aeruginosa clinical isolates under antimicrobial selective pressure and make aware of the emergence of an uncommon phenotype among P. aeruginosa clinical isolates.
Descritores: Pseudomonas aeruginosa/efeitos dos fármacos
Carbapenêmicos/farmacologia
Cefalosporinas/farmacologia
Resistência beta-Lactâmica/genética
Antibacterianos/farmacologia
-Fenótipo
Pseudomonas aeruginosa/enzimologia
Pseudomonas aeruginosa/genética
Espectrofotometria Ultravioleta
Proteínas da Membrana Bacteriana Externa
Proteínas de Bactérias/metabolismo
beta-Lactamases/metabolismo
Brasil
DNA Bacteriano
Testes de Sensibilidade Microbiana
Eletroforese em Gel de Campo Pulsado
Análise de Sequência de DNA
Porinas/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Humanos
Responsável: BR1.1 - BIREME


  10 / 598 LILACS  
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Belone, Andrea de Faria Fernandes
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Id: biblio-839189
Autor: Azevedo, Michelle de Campos Soriani; Ramuno, Natália Mortari; Fachin, Luciana Raquel Vincenzi; Tassa, Mônica; Rosa, Patrícia Sammarco; Belone, Andrea de Faria Fernandes; Diório, Suzana Madeira; Soares, Cleverson Teixeira; Garlet, Gustavo Pompermaier; Trombone, Ana Paula Favaro.
Título: qPCR detection of Mycobacterium leprae in biopsies and slit skin smear of different leprosy clinical forms
Fonte: Braz. j. infect. dis;21(1):71-78, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract Leprosy, whose etiological agent is Mycobacterium leprae, is a chronic infectious disease that mainly affects the skin and peripheral nervous system. The diagnosis of leprosy is based on clinical evaluation, whereas histopathological analysis and bacilloscopy are complementary diagnostic tools. Quantitative PCR (qPCR), a current useful tool for diagnosis of infectious diseases, has been used to detect several pathogens including Mycobacterium leprae. The validation of this technique in a robust set of samples comprising the different clinical forms of leprosy is still necessary. Thus, in this study samples from 126 skin biopsies (collected from patients on all clinical forms and reactional states of leprosy) and 25 slit skin smear of leprosy patients were comparatively analyzed by qPCR (performed with primers for the RLEP region of M. leprae DNA) and routine bacilloscopy performed in histological sections or in slit skin smear. Considering clinical diagnostic as the gold standard, 84.9% of the leprosy patients were qPCR positive in skin biopsies, resulting in 84.92% sensitivity, with 84.92 and 61.22% positive (PPV) and negative (NPV) predictive values, respectively. Concerning bacilloscopy of histological sections (BI/H), the sensitivity was 80.15% and the PPV and NPV were 80.15 and 44.44%, respectively. The concordance between qPCR and BI/H was 87.30%. Regarding the slit skin smear, 84% of the samples tested positive in the qPCR. Additionally, qPCR showed 100% specificity, since all samples from different mycobacteria, from healthy individuals, and from other granulomatous diseases presented negative results. In conclusion, the qPCR technique for detection of M. leprae using RLEP primers proved to be specific and sensitive, and qPCR can be used as a complementary test to diagnose leprosy irrespective of the clinical form of disease.
Descritores: Pele/microbiologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Hanseníase/microbiologia
Mycobacterium leprae/isolamento & purificação
-Valores de Referência
Pele/patologia
Biópsia
DNA Bacteriano/isolamento & purificação
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Primers do DNA/isolamento & purificação
Hanseníase/patologia
Mycobacterium leprae/genética
Limites: Humanos
Tipo de Publ: Estudo de Validação
Responsável: BR1.1 - BIREME



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