Base de dados : LILACS
Pesquisa : D13.444.308.212 [Categoria DeCS]
Referências encontradas : 571 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 58 ir para página                         

  1 / 571 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951818
Autor: Boujelben, Ines; Gdoura, Radhouane; Hammami, Adnane.
Título: A broad-range PCR technique for the diagnosis of infective endocarditis
Fonte: Braz. j. microbiol;49(3):534-543, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A broad-range PCR technique was selected and evaluated in terms of its efficiency in the diagnosis of endocarditis using 19 heart valves from patients undergoing cardiovascular surgeries at the Habib Bourguiba Hospital of Sfax, Tunisia, on the grounds of suspected IE. The results demonstrated the efficiency of this technique particularly in cases involving a limited number of bacteria since it helped to increase detection sensitivity. The technique proved to be efficient, particularly, in the bacteriological diagnosis of IE in contexts involving negative results from conventional culture methods and other contexts involving bacterial species that were not amenable to identification by phenotypic investigations. Indeed, the sequencing of the partial 16S ribosomal RNA gene revealed the presence of Bartonella henselae, Enterobacter sp., and Streptococcus pyogenes in three heart valves with the negative culture. It should be noted that the results obtained from the polymerase chain reaction-sequencing identification applied to the heart valve and the strain isolated from the same tissue were not consistent with the ones found by the conventional microbiological methods in the case of IE caused by Gemella morbillorum. In fact, the results from the molecular identification revealed the presence of Lactobacillus jensenii. Overall, the results have revealed that the proposed method is sensitive, reliable and might open promising opportunities for the early diagnosis of IE.
Descritores: Bactérias/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Endocardite/microbiologia
Endocardite Bacteriana/microbiologia
-Filogenia
Bactérias/classificação
Bactérias/genética
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Endocardite/diagnóstico
Endocardite Bacteriana/diagnóstico
Valvas Cardíacas/microbiologia
Meia-Idade
Limites: Seres Humanos
Masculino
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


  2 / 571 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951804
Autor: Bakhshi, Bita; Afshari, Nasim; Fallah, Fatemeh.
Título: Enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis as a reliable evidence for suspected Shigella spp. outbreaks
Fonte: Braz. j. microbiol;49(3):529-533, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Background Shigellosis remains a serious public health problem and an important cause of morbidity and mortality worldwide. The aim of this study was to characterize fliC and the genetic relatedness of Shigella spp. isolated during a one-year period from children in a suspected outbreak in Tehran, Iran. Methods and results Fifty Shigella spp. were isolated from 3779 stool samples of children with diarrhea (prevalence rate: 1.32%). Among the isolates, 92% were characterized as Shigella sonnei, while 6% and 2% were identified as S. flexneri and S. boydii, respectively. S. dysenteriae was not recovered from the patients. All isolates were negative for fliC except for Shigella standard strains. The enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) profiles allowed differentiating the 50 isolates into 5 ERIC types, which were grouped into five clusters (ET1-ET5). Computer-assisted clustering of the strains showed a high degree of similarity among the isolates. Conclusion In conclusion, given the clonal correlation of the Shigella strains isolated in this study and the lack of fliC among them, we propose that probably a single or limited fliC-defected Shigella clone spread and caused the outbreak.
Descritores: Shigella/isolamento & purificação
Surtos de Doenças
DNA Intergênico/genética
Disenteria Bacilar/microbiologia
-Filogenia
Shigella/classificação
Shigella/genética
DNA Bacteriano/genética
Reação em Cadeia da Polimerase
Disenteria Bacilar/epidemiologia
Flagelina/genética
Irã (Geográfico)/epidemiologia
Limites: Seres Humanos
Masculino
Feminino
Pré-Escolar
Criança
Responsável: BR1.1 - BIREME


  3 / 571 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951798
Autor: Rodrigues, Dalila Ribeiro; Silva, Aleksandro Ferreira da; Cavalcanti, Maria Idaline Pessoa; Escobar, Indra Elena Costa; Fraiz, Ana Carla Resende; Ribeiro, Paula Rose de Almeida; Ferreira Neto, Reginaldo Alves; Freitas, Ana Dolores Santiago de; Fernandes-Júnior, Paulo Ivan.
Título: Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest
Fonte: Braz. j. microbiol;49(3):503-512, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: Brazilian Council for Scientific and Technological Development.
Resumo: Abstract Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic genes. The bacteria showed a high variable metabolic profile. Bradyrhizobium (6), Rhizobium (3) and Paraburkholderia (1) were identified, differing from their geographic origin. The isolates grew up to 45 °C to 0.51 mol L-1 of NaCl. Bacteria which produced more auxin in the medium with l-tryptophan and two Rhizobium and one Bradyrhizobium were phosphate solubilizers. All bacteria nodulated and ESA 90 (Rhizobium sp.) plus ESA 96 (Paraburkholderia sp.) were more efficient symbiotically. Diverse and efficient rhizobia inhabit the soils of Caatinga dry forests, with the bacterial differentiation by the sampling sites.
Descritores: Rhizobium/fisiologia
Simbiose
Bradyrhizobium/fisiologia
Erythrina/microbiologia
-Fenótipo
Filogenia
Rhizobium/isolamento & purificação
Rhizobium/genética
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Cloreto de Sódio/metabolismo
Florestas
Bradyrhizobium/isolamento & purificação
Bradyrhizobium/genética
Erythrina/fisiologia
Responsável: BR1.1 - BIREME


  4 / 571 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-951793
Autor: Wu, Xiukun; Zhang, Gaosen; Zhang, Wei; Liu, Guangxiu; Chen, Tuo; Wang, Yun; Long, Haozhi; Tai, Xisheng; Zhang, Baogui; Li, Zhongqin.
Título: Variations in culturable bacterial communities and biochemical properties in the foreland of the retreating Tianshan No. 1 glacier
Fonte: Braz. j. microbiol;49(3):443-451, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: Natural Science Foundation of China; . National Basic Research Program (973) of China; . China Postdoctoral Science Fund.
Resumo: Abstract As a glacier retreats, barren areas are exposed, and these barren areas are ideal sites to study microbial succession. In this study, we characterized the soil culturable bacterial communities and biochemical parameters of early successional soils from a receding glacier in the Tianshan Mountains. The total number of culturable bacteria ranged from 2.19 × 105 to 1.30 × 106 CFU g-1 dw and from 9.33 × 105 to 2.53 × 106 CFU g-1 dw at 4 °C and 25 °C, respectively. The number of culturable bacteria in the soil increased at 25 °C but decreased at 4 °C along the chronosequence. The total organic carbon, total nitrogen content, and enzymatic activity were relatively low in the glacier foreland. The number of culturable bacteria isolated at 25 °C was significantly positively correlated with the TOC and TN as well as the soil urease, protease, polyphenoloxidase, sucrase, catalase, and dehydrogenase activities. We obtained 358 isolates from the glacier foreland soils that clustered into 35 groups using amplified ribosomal DNA restriction analysis. These groups are affiliated with 20 genera that belong to six taxa, namely, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Bacteroides, and Deinococcus-Thermus, with a predominance of members of Actinobacteria and Proteobacteria in all of the samples. A redundancy analysis showed that the bacterial succession was divided into three periods, an early stage (10a), a middle stage (25-74a), and a late stage (100-130a), with the total number of culturable bacteria mainly being affected by the soil enzymatic activity, suggesting that the microbial succession correlated with the soil age along the foreland.
Descritores: Bactérias/isolamento & purificação
Camada de Gelo/microbiologia
Camada de Gelo/química
-Filogenia
Solo/química
Microbiologia do Solo
Bactérias/classificação
Bactérias/crescimento & desenvolvimento
Bactérias/genética
DNA Bacteriano/genética
DNA Ribossômico/genética
RNA Ribossômico 16S/genética
China
Análise de Sequência de DNA
Nitrogênio/análise
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  5 / 571 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974306
Autor: Dealtry, Simone; Ghizelini, Angela Michelato; Mendonça-Hagler, Leda C. S; Chaloub, Ricardo Moreira; Reinert, Fernanda; Campos, Tácio M. P. de; Gomes, Newton C. M; Smalla, Kornelia.
Título: Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana
Fonte: Braz. j. microbiol;49(4):757-769, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Deutsche Forschungsgemeinschaft; . FAPERJ-Brazil.
Resumo: ABSTRACT Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while the bacterial community was rather stable, pronounced shifts were observed in the abundance of bacteria carrying plasmids. A PCR-Southern blot hybridization analysis indicated an increase in the abundance of IncP-9 catabolic plasmids. Denaturing gradient gel electrophoresis of naphthalene dioxygenase (ndo) genes amplified from cDNA (RNA) indicated the dominance of a specific ndo gene in the inoculated petroleum amendment treatment. The petroleum hydrocarbon degrading consortium characterization indicated the prevalence of bacteria assigned to Pseudomonas spp., Comamonas spp. and Ochrobactrum spp. IncP-9 plasmids were detected for the first time in Comamonas sp. and Ochrobactrum spp., which is a novelty of this study.
Descritores: Bactérias/isolamento & purificação
Bactérias/metabolismo
Avicennia/microbiologia
Hidrocarbonetos/metabolismo
-Plasmídeos/genética
Plasmídeos/metabolismo
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
Bactérias/classificação
Bactérias/genética
Biodegradação Ambiental
DNA Bacteriano/genética
Petróleo/análise
RNA Ribossômico 16S/genética
Poluição por Petróleo/análise
Avicennia/metabolismo
Rizosfera
Responsável: BR1.1 - BIREME


  6 / 571 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974305
Autor: Leite, Jakson; Passos, Samuel Ribeiro; Simões-Araújo, Jean Luiz; Rumjanek, Norma Gouvêa; Xavier, Gustavo Ribeiro; Zilli, Jerri Édson.
Título: Genomic identification and characterization of the elite strains Bradyrhizobium yuanmingense BR 3267 and Bradyrhizobium pachyrhizi BR 3262 recommended for cowpea inoculation in Brazil
Fonte: Braz. j. microbiol;49(4):703-713, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT The leguminous inoculation with nodule-inducing bacteria that perform biological nitrogen fixation is a good example of an "eco-friendly agricultural practice". Bradyrhizobium strains BR 3267 and BR 3262 are recommended for cowpea (Vigna unguiculata) inoculation in Brazil and showed remarkable responses; nevertheless neither strain was characterized at species level, which is our goal in the present work using a polyphasic approach. The strains presented the typical phenotype of Bradyrhizobium with a slow growth and a white colony on yeast extract-mannitol medium. Strain BR 3267 was more versatile in its use of carbon sources compared to BR 3262. The fatty acid composition of BR 3267 was similar to the type strain of Bradyrhizobium yuanmingense; while BR 3262 was similar to Bradyrhizobium elkanii and Bradyrhizobium pachyrhizi. Phylogenetic analyses based on 16S rRNA and three housekeeping genes placed both strains within the genus Bradyrhizobium: strain BR 3267 was closest to B. yuanmingense and BR 3262 to B. pachyrhizi. Genome average nucleotide identity and DNA-DNA reassociation confirmed the genomic identification of B. yuanmingense BR 3267 and B. pachyrhizi BR 3262. The nodC and nifH gene analyses showed that strains BR 3267 and BR 3262 hold divergent symbiotic genes. In summary, the results indicate that cowpea can establish effective symbiosis with divergent bradyrhizobia isolated from Brazilian soils.
Descritores: Bradyrhizobium/isolamento & purificação
Bradyrhizobium/genética
Inoculantes Agrícolas/isolamento & purificação
Inoculantes Agrícolas/genética
Vigna/microbiologia
-Filogenia
Simbiose
Brasil
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Genoma Bacteriano
Evolução Molecular
Bradyrhizobium/classificação
Bradyrhizobium/fisiologia
Genômica
Nódulos Radiculares de Plantas/microbiologia
Inoculantes Agrícolas/classificação
Inoculantes Agrícolas/fisiologia
Vigna/fisiologia
Responsável: BR1.1 - BIREME


  7 / 571 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974298
Autor: Vásquez-Ponce, Felipe; Higuera-Llantén, Sebastián; Pavlov, María S; Marshall, Sergio H; Olivares-Pacheco, Jorge.
Título: Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica
Fonte: Braz. j. microbiol;49(4):695-702, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Pontificia Universidad Católica de Valparaíso; . Programa de Investigación Asociativa.
Resumo: ABSTRACT Antarctica harbors a great diversity of microorganisms, including bacteria, archaea, microalgae and yeasts. The Pseudomonas genus is one of the most diverse and successful bacterial groups described to date, but only eight species isolated from Antarctica have been characterized. Here, we present three potentially novel species isolated on King George Island. The most abundant isolates from four different environments, were genotypically and phenotypically characterized. Multilocus sequence analysis and 16S rRNA gene analysis of a sequence concatenate for six genes (16S, aroE, glnS, gyrB, ileS and rpoD), determined one of the isolates to be a new Pseudomonas mandelii strain, while the other three are good candidates for new Pseudomonas species. Additionally, genotype analyses showed the three candidates to be part of a new subgroup within the Pseudomonas fluorescens complex, together with the Antarctic species Pseudomonas antarctica and Pseudomonas extremaustralis. We propose terming this new subgroup P. antarctica. Likewise, phenotypic analyses using API 20 NE and BIOLOG® corroborated the genotyping results, confirming that all presented isolates form part of the P. fluorescens complex. Pseudomonas genus research on the Antarctic continent is in its infancy. To understand these microorganisms' role in this extreme environment, the characterization and description of new species is vital.
Descritores: Filogenia
Pseudomonas/isolamento & purificação
Pseudomonas/classificação
-Fenótipo
Pseudomonas/genética
Microbiologia do Solo
DNA Bacteriano/genética
DNA Ribossômico/genética
RNA Ribossômico 16S/genética
Tipagem de Sequências Multilocus
Ilhas
Genótipo
Regiões Antárticas
Responsável: BR1.1 - BIREME


  8 / 571 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974296
Autor: Goes, Kelly Campos Guerra Pinheiro de; Lovato, Gisele Milani; Andrade, Diva S.
Título: Composition of bacterial community in enrichment cultures of shale by-products from Irati Formation, Brazil
Fonte: Braz. j. microbiol;49(4):742-748, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: Fundação Araucária.
Resumo: ABSTRACT We examined microbial communities from enriched fine and retorted shale particles using sequencing of V4 variable region of 16S rRNA. High number of microbial genera was found in both enriched shale by-products that were dominate by Actinobacteria, Firmicutes and Proteobacteria, showing differences due to microbial colonization after the pyrolysis process.
Descritores: Bactérias/isolamento & purificação
Resíduos/análise
Sedimentos Geológicos/microbiologia
Microbiota
-Filogenia
Bactérias/classificação
Bactérias/genética
Brasil
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Sedimentos Geológicos/química
Biodiversidade
Responsável: BR1.1 - BIREME


  9 / 571 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Vasconcellos, Silvio Arruda
Texto completo
Id: biblio-974290
Autor: Karcher, Daniel; Grenfell, Rafaella C; Moreno, Andrea Micke; Moreno, Luisa Zanolli; Vasconcellos, Silvio Arruda; Heinemann, Marcos B; Almeida Junior, Joao N. de; Juliano, Luiz; Juliano, Maria A.
Título: Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry
Fonte: Braz. j. microbiol;49(4):900-908, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: ABSTRACT Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection of well-identified Brazilian isolates kept in the Bacterial Zoonosis Laboratory at the Veterinary Preventive Medicine and Animal Health Department at Sao Paulo University. In addition, L. interrogans and L. kirschneri were differentiated using an in-depth mass spectrometry analysis with ClinProTools™ software. In conclusion, our in-house reference spectra database has the necessary accuracy to differentiate pathogenic and non-pathogenic species and to distinguish L. interrogans and L. kirschneri.
Descritores: Técnicas de Tipagem Bacteriana/métodos
Espectrometria de Massas em Tandem/métodos
Leptospira/isolamento & purificação
Leptospirose/microbiologia
-Brasil
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Leptospira/classificação
Leptospira/genética
Leptospira/química
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


  10 / 571 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-974289
Autor: Charousová, Ivana; Medo, Juraj; Hleba, Lukáš; Javoreková, Soňa.
Título: Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics
Fonte: Braz. j. microbiol;49(4):816-822, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Slovak Academy of Sciences.
Resumo: ABSTRACT Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolates matched those described for members of the genus Streptomyces. DK-15 strain exhibited the highest 16S rRNA gene sequence similarity to Streptomyces globosus DSM-40815 (T) and Streptomyces toxytricini DSM-40178 (T) and ST-13 strain to Streptomyces ederensis DSM-40741 (T) and Streptomyces phaeochromogenes DSM-40073 (T). For the proper identification, MALDI-TOF/MS profile of whole-cell proteins led to the identification of S. globosus DK-15 (accession number: KX527570) and S. ederensis ST13 (accession number: KX527568). To our knowledge, there is no report about the production of these antibiotics by S.globosus and S. ederensis, thus isolates DK15 and ST13 identified as S. globosus DK-15 and S.ederensis ST-13 can be considered as new sources of these unique antibacterial metabolites.
Descritores: Streptomyces/isolamento & purificação
Streptomyces/metabolismo
Antibacterianos/biossíntese
-Filogenia
Piridonas/metabolismo
Microbiologia do Solo
Streptomyces/classificação
Streptomyces/genética
Benzo(a)Antracenos/metabolismo
DNA Bacteriano/genética
Técnicas de Tipagem Bacteriana
Responsável: BR1.1 - BIREME



página 1 de 58 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde