Base de dados : LILACS
Pesquisa : D13.444.308.212 [Categoria DeCS]
Referências encontradas : 550 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 55 ir para página                         

  1 / 550 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-777090
Autor: Khosravi, Azar Dokht; Hoveizavi, Hajar; Mohammadian, Ali; Farahani, Abbas; Jenabi, Atefeh.
Título: Genotyping of multidrug-resistant strains of Pseudomonas aeruginosa isolated from burn and wound infections by ERIC-PCR
Fonte: Acta cir. bras;31(3):206-211, Mar. 2016. tab, graf.
Idioma: en.
Projeto: Ahvaz Jundishapur University of Medical Sciences.
Resumo: ABSTRACT PURPOSE: To determine the genetic diversity of MDR P. aeruginosa strains isolated from burn and wound infections in Ahvaz, Iran, by ERIC-PCR. METHODS: From total 99 strains of P. aeruginosa defined as MDR by using drug susceptibility testing, 66 were subjected to ERIC-PCR analysis, comprises 53 strains isolated from burn infection, and 13 randomly selected strains from wound infection with higher resistance to combinations of more numbers of drugs. RESULTS: Eight clusters (I to VIII), and 50 single clones were generated for tested MDR isolates analyzed by ERIC-PCR. The high heterogeneity was observed among the isolates from burn infections including 16 isolates which were categorized in eight clusters and 37 single clones. The isolates in clusters II, III, VI, VIII showed 100% similarity. CONCLUSIONS: The high level of genotypic heterogeneity in P. aeruginosa strains demonstrated no genetic correlation between them. Extremely high drug resistance in isolates from burn, suggests that efficient control measures and proper antibiotic policy should be observed.
Descritores: Pseudomonas aeruginosa/genética
Infecções por Pseudomonas/microbiologia
Infecção dos Ferimentos/microbiologia
Queimaduras/microbiologia
DNA Bacteriano/genética
Farmacorresistência Bacteriana Múltipla/genética
-Pseudomonas aeruginosa/isolamento & purificação
Sequências Repetitivas de Ácido Nucleico/genética
Reação em Cadeia da Polimerase
Genótipo
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 550 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Rodrigues, Dália dos Prazeres
Leal, Nilma Cintra
Texto completo
Id: lil-426797
Autor: Theophilo, Grace Nazareth Diogo; Rodrigues, Dália dos Prazeres; Leal, Nilma Cintra; Hofer, Ernesto.
Título: Distribution of virulence markers in clinical and environmental Vibrio cholerae non-O1/non-O139 strains isolated in Brazil from 1991 to 2000
Fonte: Rev. Inst. Med. Trop. Säo Paulo;48(2):65-70, Mar,-Apr. 2006. tab.
Idioma: en.
Projeto: National Council for Research Support.
Resumo: Cento e setenta e nove amostras de V. cholerae não O1/não O139, isoladas de casos clínicos (139) e de meio ambiente (40), no período de 1991 a 2000 no Brasil, foram caracterizadas antigenicamente pelo National Institute of Health (Japão) e investigadas quanto ao seu potencial genético de virulência, representado pelos genes ctxA, zot, ace e tcpA. As análises fenotípicas revelaram extraordinária diversidade antigênica, com a ocorrência de 54 diferentes sorogrupos, com prevalência para O26 (7,8%). A técnica de PCR, empregada na detecção dos genes localizados no elemento genético CTX (ctxA, zot, ace) e na Ilha de Patogenicidade de Vibrio-VPI (tcpA), possibilitou a identificação de 27 cepas contendo qualquer um desses genes. O gene ctxA (codificador da sub-unidade A de CT), só foi evidenciado no sorogrupo O26, sendo também o único capaz de se apresentar com o cassete de virulência de forma intacta. Com base nos resultados obtidos deste estudo preliminar, admite-se a hipótese da potencialidade destas cepas, evoluir para raças epidêmicas.
Descritores: Proteínas de Bactérias/genética
DNA Bacteriano/genética
Genes Bacterianos/genética
/genética
VIBRIO CHOLERAE OACHONDROPLASIA/genética
Vibrio cholerae não O1/genética
-Brasil
Marcadores Genéticos
Reação em Cadeia da Polimerase
Técnica de Amplificação ao Acaso de DNA Polimórfico
/patogenicidade
VIBRIO CHOLERAE OACHONDROPLASIA/patogenicidade
Vibrio cholerae não O1/patogenicidade
Virulência/genética
Limites: Seres Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 550 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-426796
Autor: Avila-Campos, Mario J; Rivera, Irma N; Nakano, Viviane.
Título: Genetic diversity of oral Fusobacterium nucleatum isolated from patients with different clinical conditions
Fonte: Rev. Inst. Med. Trop. Säo Paulo;48(2):59-63, Mar,-Apr. 2006. ilus, tab.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Neste estudo foi avaliada a diversidade genética de 23 amostras de Fusobacterium nucleatum isoladas da cavidade bucal de 15 pacientes com doença periodontal, de oito cepas isoladas de sete indivíduos sadios, de nove isoladas de nove pacientes com AIDS e de duas isoladas de dois macacos Cebus apella. Pela ação da enzima EcoRI sobre o DNA bacteriano foram reconhecidos 28 ribotipos agrupados de A a J. Os isolados testados formaram 24 ribotipos os quais foram contidos nos grupos A, B, C, D, E e F, e as três cepas de referência e dois isolados clínicos de A. actinomycetemcomitans e E. coli CDC formaram quatro diferentes ribotipos contidos nos grupos G, H, I e J. Em adição, as nove cepas de F. nucleatum isoladas de pacientes com AIDS, seis pertenciam ao grupo C e três ao grupo D. Usando-se a ribotipagem foi possível distinguir F. nucleatum isolados de diferentes origens.
Descritores: Variação Genética
DNA Bacteriano/análise
Infecções por Fusobacterium/microbiologia
Fusobacterium nucleatum/genética
Doenças Periodontais/microbiologia
-Infecções Oportunistas Relacionadas com a AIDS/microbiologia
Técnicas de Tipagem Bacteriana
Southern Blotting
Cebus/microbiologia
Ribotipagem
Limites: Seres Humanos
Animais
Adolescente
Adulto
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 550 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-779908
Autor: BINTA, Buhle; PATEL, Mrudula.
Título: Detection of cfxA2, cfxA3, and cfxA6 genes in beta-lactamase producing oral anaerobes
Fonte: J. appl. oral sci;24(2):142-147, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Projeto: University of Copenhagen.
Resumo: ABSTRACT Purpose The aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxA and BlaTEM genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. Material and Methods Periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plates with and without β-lactam antibiotics. Presumptive β-lactamase-producing isolates were evaluated for definite β-lactamase production using the nitrocefin slide method and identified using the API Rapid 32A system. Antimicrobial susceptibility was performed using disc diffusion and microbroth dilution tests as described by CLSI Methods. Isolates were screened for the presence of the β-lactamase-TEM (BlaTEM) and β-lactamase-cfxA genes using Polymerase Chain Reaction (PCR). Amplified PCR products were sequenced and the cfxA gene was characterized using Genbank databases. Results Seventy five percent of patients carried two species of β-lactamase-producing anaerobic bacteria that comprised 9.4% of the total number of cultivable bacteria. Fifty one percent of β-lactamase-producing strains mainly Prevotella, Porphyromonas, and Bacteroides carried the cfxA gene, whereas none of them carried blaTEM. Further characterization of the cfxA gene showed that 76.7% of these strains carried the cfxA2 gene, 14% carried cfxA3, and 9.3% carried cfxA6. The cfxA6 gene was present in three Prevotella spp. and in one Porphyromonas spp. Strains containing cfxA genes (56%) were resistant to the β-lactam antibiotics. Conclusion This study indicates that there is a high prevalence of the cfxA gene in β-lactamase-producing anaerobic oral bacteria, which may lead to drug resistance and treatment failure.
Descritores: Bolsa Periodontal/microbiologia
Bactérias Anaeróbias/isolamento & purificação
Bactérias Anaeróbias/metabolismo
beta-Lactamases/biossíntese
-Valores de Referência
beta-Lactamases/isolamento & purificação
beta-Lactamases/genética
DNA Bacteriano
Testes de Sensibilidade Microbiana
Reação em Cadeia da Polimerase
Resistência beta-Lactâmica
Periodontite Crônica/microbiologia
Boca/microbiologia
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


  5 / 550 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-779905
Autor: GONÇALVES, Cristiane; SOARES, Geisla Mary S; FAVERI, Marcelo; PÉREZ-CHAPARRO, Paula Juliana; LOBÃO, Eduardo; FIGUEIREDO, Luciene Cristina; BACCELLI, Gustavo Titonele; FERES, Magda.
Título: Association of three putative periodontal pathogens with chronic periodontitis in Brazilian subjects
Fonte: J. appl. oral sci;24(2):181-185, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Projeto: São Paulo Research Foundation.
Resumo: ABSTRACT Objective The aim of this study was to evaluate the association of Porphyromonas endodontalis, Filifactor alocis and Dialister pneumosintes with the occurrence of periodontitis. Material and Methods Thirty subjects with chronic periodontitis (ChP) and 10 with periodontal health (PH) were included in the study. Nine subgingival biofilm samples were collected as follows: i) PH group - from the mesial/buccal aspect of each tooth in two randomly chosen contralateral quadrants; ii) ChP group - from three sites in each of the following probing depth (PD) categories: shallow (≤3 mm), moderate (4-6 mm) and deep (≥7 mm). Checkerboard DNA-DNA hybridization was used to analyze the samples. Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test). We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test). In addition, the prevalence and levels of D. pneumosintes, and especially of F. alocis were very low in healthy subjects (0.12x105 and 0.01x105, respectively). Conclusion F. alocis and D. pneumosintes might be associated with the etiology of ChP, and their role in the onset and progression of this infection should be further investigated. The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.
Descritores: Peptostreptococcus/patogenicidade
Porphyromonas endodontalis/patogenicidade
Veillonellaceae/patogenicidade
Periodontite Crônica/microbiologia
-Peptostreptococcus/isolamento & purificação
Brasil
DNA Bacteriano
Contagem de Colônia Microbiana
Sondas de DNA
Estudos de Casos e Controles
Estatísticas não Paramétricas
Biofilmes
Porphyromonas endodontalis/isolamento & purificação
Placa Dentária/microbiologia
Veillonellaceae/isolamento & purificação
Gengiva/microbiologia
Limites: Seres Humanos
Masculino
Feminino
Adulto
Meia-Idade
Responsável: BR1.1 - BIREME


  6 / 550 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1020078
Autor: Brasiliense, Danielle; Cayô, Rodrigo; Streling, Ana Paula; Nodari, Carolina S; Barata, Rafael R; Lemos, Poliana S; Massafra, Janaina M; Correa, Yan; Magalhães, Igor; Gales, Ana C; Sodré, Roberta.
Título: Diversity of metallo-B-lactamase-encoding genes found in distinct species of Acinetobacter isolated from the Brazilian Amazon Region
Fonte: Mem. Inst. Oswaldo Cruz;114:e190020, 2019. tab, graf.
Idioma: en.
Projeto: CAPES; . APS; . CSN; . DS-CAPES; . CNPq; . ACG.
Resumo: BACKGROUND The multidrug resistance (MDR) phenotype is frequently observed in Acinetobacter baumannii, the most clinically relevant pathogenic species of its genus; recently, other species belonging to the A. calcoaceticus-A. baumannii complex have emerged as important MDR nosocomial pathogens. OBJECTIVES The present study aimed to verify the occurrence of metallo-β-lactamase genes among distinct Acinetobacter species in a hospital located in the Brazilian Amazon Region. METHODS Antimicrobial susceptibility profiles were determined by broth microdilution. The genetic relationships among these isolates were assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Pyrosequencing reads of plasmids carrying the bla NDM-1 gene were generated using the Ion Torrent™ platform sequencing. FINDINGS A total of six isolates carried bla NDM-1: A. baumannii (n = 2), A. nosocomialis (n = 3), and A. pittii (n = 1); three carried bla IMP-1: A. baumannii, A. nosocomialis, and A. bereziniae. Resistance to colistin was observed for an NDM-1-producing A. nosocomialis isolate. Diverse PFGE patterns and sequence types were found among A. nosocomialis and A. baumannii isolates. The bla NDM-1 sequence was inserted in a Tn125 transposon, while the bla IMP-1 was found as a gene cassette of the class 1 integron In86. MAIN CONCLUSIONS To the best of our knowledge, this is the first report describing the dissemination of bla NDM-1 among distinct Acinetobacter species recovered from the same hospital in South America.
Descritores: Acinetobacter/isolamento & purificação
Acinetobacter/efeitos dos fármacos
Acinetobacter/química
beta-Lactamases/isolamento & purificação
DNA Bacteriano
Resistência a Medicamentos
Carbapenêmicos/farmacologia
Eletroforese
Antibacterianos/farmacologia
-Brasil
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  7 / 550 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Campos, Carlos Eduardo Dias
Suffys, Philip N
Texto completo
Id: biblio-955125
Autor: Machado, Edson; Vasconcellos, Sidra Ezidio Gonçalves; Cerdeira, Camillo; Gomes, Lia Lima; Junqueira, Ricardo; Carvalho, Luciana Distasio de; Ramos, Jesus Pais; Redner, Paulo; Campos, Carlos Eduardo Dias; Caldas, Paulo Cesar de Souza; Gomes, Ana Paula Chaves Sobral; Goldenberg, Telma; Montes, Fatima Fandinho; Mello, Fernanda Carvalho de Queiroz; Mussi, Vinicius de Oliveira; Lasunskaia, Elena; Soolingen, Dick van; Miranda, Antonio Basílio de; Rigouts, Leen; Jong, Bouke C de; Meehan, Conor J; Catanho, Marcos; Suffys, Philip N.
Título: Whole genome sequence of Mycobacterium kansasii isolates of the genotype 1 from Brazilian patients with pulmonary disease demonstrates considerable heterogeneity
Fonte: Mem. Inst. Oswaldo Cruz;113(9):e180085, 2018. tab, graf.
Idioma: en.
Projeto: CNPq.
Resumo: Mycobacterium kansasii is an opportunistic pathogen and one of the most commonly encountered species in individuals with lung disease. We here report the complete genome sequence of 12 clinical isolates of M. kansasii from patients with pulmonary disease in Brazil.
Descritores: DNA Bacteriano
Genoma Bacteriano/genética
Mycobacterium kansasii/genética
-Gráficos por Computador
Responsável: BR1.1 - BIREME


  8 / 550 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-894856
Autor: Arraes, Maria Luisa Bezerra de Macedo; Holanda, Maísa Viana de; Lima, Luana Nepomuceno Gondim Costa; Sabadia, José Antônio Beltrão; Duarte, Cynthia Romariz; Almeida, Rosa Livia Freitas; Kendall, Carl; Kerr, Ligia Regina Sansigolo; Frota, Cristiane Cunha.
Título: Natural environmental water sources in endemic regions of northeastern Brazil are potential reservoirs of viable Mycobacterium leprae
Fonte: Mem. Inst. Oswaldo Cruz;112(12):805-811, Dec. 2017. tab, graf.
Idioma: en.
Projeto: CNPq; . Water and Public Health.
Resumo: BACKGROUND The detection of live Mycobacterium leprae in soil and animals other than humans suggests that the environment plays a role in the transmission of leprosy. OBJECTIVE The objective of this study was to investigate the presence of viable M. leprae in natural water sources used by the local population in five municipalities in the state of Ceará, northeastern Brazil. METHODS Samples were collected from 30 different sources. Viable bacilli were identified by reverse transcriptase polymerase chain reaction (PCR) of the M. leprae gyrA gene and sequencing of the PCR products. Physicochemical properties of each water source were also assessed. FINDINGS M. leprae gyrA mRNA was found in 23 (76.7%) of the water sources. No association was found between depth of the water and sample positivity, nor was there any association between the type of water used by the population and sample positivity. An association between viable M. leprae and temperature and pH was found. Georeferencing showed a relation between the residences of leprosy cases and water source containing the bacterium. MAIN CONCLUSIONS The finding of viable M. leprae in natural water sources associated with human contact suggests that the environment plays an important role in maintaining endemic leprosy in the study region.
Descritores: DNA Bacteriano/genética
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Mycobacterium leprae/isolamento & purificação
Mycobacterium leprae/genética
-Microbiologia da Água
Brasil
Reservatórios de Doenças
Genótipo
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  9 / 550 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-889237
Autor: Yasir, Muhammad.
Título: Analysis of bacterial communities and characterization of antimicrobial strains from cave microbiota
Fonte: Braz. j. microbiol;49(2):248-257, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah.
Resumo: Abstract In this study for the first-time microbial communities in the caves located in the mountain range of Hindu Kush were evaluated. The samples were analyzed using culture-independent (16S rRNA gene amplicon sequencing) and culture-dependent methods. The amplicon sequencing results revealed a broad taxonomic diversity, including 21 phyla and 20 candidate phyla. Proteobacteria were dominant in both caves, followed by Bacteroidetes, Actinobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, and the archaeal phylum Euryarchaeota. Representative operational taxonomic units from Koat Maqbari Ghaar and Smasse-Rawo Ghaar were grouped into 235 and 445 different genera, respectively. Comparative analysis of the cultured bacterial isolates revealed distinct bacterial taxonomic profiles in the studied caves dominated by Proteobacteria in Koat Maqbari Ghaar and Firmicutes in Smasse-Rawo Ghaar. Majority of those isolates were associated with the genera Pseudomonas and Bacillus. Thirty strains among the identified isolates from both caves showed antimicrobial activity. Overall, the present study gave insight into the great bacterial taxonomic diversity and antimicrobial potential of the isolates from the previously uncharacterized caves located in the world's highest mountains range in the Indian sub-continent.
Descritores: Bactérias/isolamento & purificação
Bactérias/classificação
Microbiologia Ambiental
Biota
Antibiose
-Paquistão
Filogenia
Bactérias/crescimento & desenvolvimento
Bactérias/genética
DNA Bacteriano/genética
DNA Bacteriano/química
DNA Ribossômico/genética
DNA Ribossômico/química
RNA Ribossômico 16S/genética
Análise por Conglomerados
Análise de Sequência de DNA
Euryarchaeota/isolamento & purificação
Euryarchaeota/classificação
Euryarchaeota/crescimento & desenvolvimento
Euryarchaeota/genética
DNA Arqueal/genética
DNA Arqueal/química
Metagenômica
Responsável: BR1.1 - BIREME


  10 / 550 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-889216
Autor: Yang, Xue-Jing; Wang, Sai; Cao, Jun-Min; Hou, Jia-Hui.
Título: Complete genome sequence of human pathogen Kosakonia cowanii type strain 888-76T
Fonte: Braz. j. microbiol;49(1):16-17, Jan.-Mar. 2018.
Idioma: en.
Projeto: Science and Technology Program of Zhejiang Province; . Zhejiang Provincial Medical and Health Science.
Resumo: ABSTRACT Kosakonia cowanii type strain 888-76T is a human pathogen which was originally isolated from blood as NIH group 42. In this study, we report the complete genome sequence of K. cowanii 888-76T. 888-76T has 1 chromosome and 2 plasmids with a total genome size of 4,857,567 bp and C+G 56.15%. This genome sequence will not only help us to understand the virulence features of K. cowanii 888-76T but also provide us the useful information for the study of evolution of Kosakonia genus.
Descritores: Genoma Bacteriano
Enterobacteriaceae/isolamento & purificação
Enterobacteriaceae/genética
Infecções por Enterobacteriaceae/microbiologia
-Filogenia
Plasmídeos/genética
Composição de Bases
DNA Bacteriano/genética
Dados de Sequência Molecular
Sequência de Bases
Enterobacteriaceae/classificação
Limites: Seres Humanos
Responsável: BR1.1 - BIREME



página 1 de 55 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde