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Id: biblio-1283791
Autor: González Bonet, Ileana; Romero Elías, María Jacqueline; Morales Mejías, Erik; Valdez Moyano, Eliana; Cofré Loyola, Cecilia; Manques Maldonado, Belarmino; Rojas Rubio, Armando.
Título: Patrón EPIYA en cepas de Helicobacter pylori CagA positivas en pacientes del Hospital Regional de Talca / EPIYA pattern in Helicobacter pylori CagA positive strains in patients of the Regional Hospital of Talca
Fonte: Rev. méd. Maule;33(1):8-13, jun. 2017. tab.
Idioma: es.
Resumo: BACKGROUND: The clinical outcome of Helicobacter pylori (H. pylori) infection has been related to the presence of CagA protein. This protein is highly polymorphic and its oncogenic ability depends on the number and type of tyrosine phosphorylation sites in the EPIYAs repeat sequences (A, B, C and D). AIM: To determine the EPIYA patterns of the CagA gene in H. pylori strains and its relationship with gastrointestinal pathology in infected patients of the Regional Hospital of Talca. MATERIAL AND METHODS: The strains were isolated from gastric biopsies and characterized by bacteriological and molecular methods. Gastrointestinal pathology was characterized by histopathological analysis. For the determination of the presence of the cagA gene and the EPIYAs standards, the conventional PCR technique was used. RESULTS: 138 DNA samples from H. pylori strains were analyzed. 92.0% (127/138) of the isolates carried the cagA gene, of which 66 (52.0%) corresponded to the EPIYA-ABC pattern, 43 (33.8%) to the EPIYA-ABCC pattern and 21 16.5%) to the EPIYA-ABCCC phosphorylation pattern. 50.4% (64/127) of cagA positive strains isolated from dyspeptic patients in the Maule region have more than two C sites of phosphorylation. The number of EPIYAs C motifs was associated with the presence of more severe histopathological damage in the gastric mucosa.
Descritores: Neoplasias Gástricas/microbiologia
Neoplasias Gástricas/patologia
Helicobacter pylori/genética
Infecções por Helicobacter/microbiologia
Infecções por Helicobacter/patologia
Motivos de Aminoácidos
-Neoplasias Gástricas/epidemiologia
Proteínas de Bactérias/genética
Biópsia
DNA Bacteriano/genética
DNA Bacteriano/química
Chile/epidemiologia
Epidemiologia Descritiva
Endoscopia do Sistema Digestório
Infecções por Helicobacter/epidemiologia
Comissão de Ética
Análise de Sequência de DNA
Antígenos de Bactérias/genética
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-961436
Autor: Wormwood, Tracy; Parra, Álvaro; Bresky, Gustavo; Madariaga, Juan A; Häberle, Sergio; Flores, Jacqueline; Bernal, Giuliano.
Título: Prevalencia de cepas cagA-positivo en la región de Coquimbo, determinada mediante nested-qPCR en muestras fecales / Frequency of CagA-positive Helicobacter pylori strains in 160 patients subjected to endoscopy
Fonte: Rev. méd. Chile;146(5):596-602, mayo 2018. tab, graf.
Idioma: es.
Projeto: CORFO.
Resumo: Background: Helicobacter pylori is the most significant pathogen associated with gastric diseases, including gastric cancer. Infected patients with strains that are CagA-positive generally have worse outcomes than those infected with CagA-negative strains. Patients infected with CagA-positive strains have a higher risk for developing gastric cancer. Aim: To determine the prevalence of CagA-positive H. pylori strains in fecal samples of patients from the Coquimbo Region of Chile, using a non-invasive, nested-qPCR method. Material and Methods: We evaluated 160 patients with gastrointestinal symptoms subjected to an upper gastrointestinal endoscopy. DNA was extracted from fecal samples and tested for the presence of H. pylori using nested-qPCR for the ureC gene, and subsequently compared with the results of histology-Giemsa stain from the patients' endoscopic biopsies. When H. pylori was found, the presence of CagA-positive strains was determined via nested-qPCR. Results: The histology-Giemsa stain was positive for H. pylori infection in 123 patients (76.9%), while the analysis of fecal samples detected H. pylori in 129 patients (80.6%). The sensitivity and specificity of nested-qPCR to detect the bacterium was 96.7 and 73.0% respectively. Among patients with the infection, 25% had CagA-positive strains. Conclusions: In this sample of patients, there is a low prevalence of CagA-positive H. pylori strains.
Descritores: Gastropatias/microbiologia
Proteínas de Bactérias/genética
DNA Bacteriano/genética
Helicobacter pylori/genética
Infecções por Helicobacter/diagnóstico
Fezes/microbiologia
Antígenos de Bactérias/genética
-Gastropatias/diagnóstico
Proteínas de Bactérias/isolamento & purificação
Reação em Cadeia da Polimerase
Endoscopia do Sistema Digestório
Sensibilidade e Especificidade
Antígenos de Bactérias/isolamento & purificação
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-890746
Autor: de Souza Carraro, Danila; Carraro, Rafael Medeiros; Campos, Silvia Vidal; Iuamoto, Leandro Ryuchi; Braga, Karina Andrighetti de Oliveira; Oliveira, Lea Campos de; Sabino, Ester Cerdeira; Rossi, Flavia; Pêgo-Fernandes, Paulo Manuel.
Título: Burkholderia cepacia, cystic fibrosis and outcomes following lung transplantation: experiences from a single center in Brazil
Fonte: Clinics;73:e166, 2018. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: OBJECTIVES: To evaluate the impact of Burkholderia cepacia complex colonization in cystic fibrosis patients undergoing lung transplantation. METHODS: We prospectively analyzed clinical data and respiratory tract samples (sputum and bronchoalveolar lavage) collected from suppurative lung disease patients between January 2008 and November 2013. We also subtyped different Burkholderia cepacia complex genotypes via DNA sequencing using primers against the recA gene in samples collected between January 2012 and November 2013. RESULTS: From 2008 to 2013, 34 lung transplants were performed on cystic fibrosis patients at our center. Burkholderia cepacia complex was detected in 13 of the 34 (38.2%) patients. Seven of the 13 (53%) strains were subjected to genotype analysis, from which three strains of B. metallica and four strains of B. cenocepacia were identified. The mortality rate was 1/13 (7.6%), and this death was not related to B. cepacia infection. CONCLUSION: The results of our study suggest that colonization by B. cepacia complex and even B. cenocepacia in patients with cystic fibrosis should not be considered an absolute contraindication to lung transplantation in Brazilian centers.
Descritores: Transplante de Pulmão/efeitos adversos
Burkholderia cepacia/isolamento & purificação
Infecções por Burkholderia/etiologia
Fibrose Cística/microbiologia
-Filogenia
Fatores de Tempo
Brasil/epidemiologia
DNA Bacteriano
Estudos Prospectivos
Análise de Regressão
Fatores de Risco
Transplante de Pulmão/mortalidade
Resultado do Tratamento
Infecções por Burkholderia/mortalidade
Fibrose Cística/cirurgia
Fibrose Cística/complicações
Fibrose Cística/mortalidade
Estimativa de Kaplan-Meier
Contraindicações de Procedimentos
Unidades de Terapia Intensiva
Tempo de Internação
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 627 LILACS  
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Teixeira, Lisete Ribeiro
ANTONANGELO, LEILA
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Id: biblio-974919
Autor: Carnevale, Gabriela Gaspar; Vargas, Francisco Suso; Caiaffa-Filho, Hélio Hehl; Acencio, Milena Marques Pagliarelli; Marçal, Lia Junqueira; Sales, Roberta Karla Barbosa; Teixeira, Lisete Ribeiro; Antonangelo, Leila.
Título: Preanalytical conditions can interfere with M. tuberculosis detection by PCR in respiratory samples
Fonte: Clinics;73:e410, 2018. tab, graf.
Idioma: en.
Resumo: OBJECTIVES: Tuberculosis is one of the most prevalent infections in humans. Although culture is the reference for diagnosis, its sensitivity is compromised, especially in paucibacillary samples. Because polymerase chain reaction (PCR) amplifies mycobacterial DNA, it is more sensitive than culture for the diagnosis of Mycobacterium tuberculosis (Mtb). However, its performance can be affected by intrinsic sample inhibitors and by the extraction/detection techniques used. METHODS: We evaluated the influence of preanalytical conditions on Mtb detection in samples of sputum (SPU), bronchoalveolar lavage (BAL), and pleural fluid (PF) using combinations of extraction/detection methods. Respiratory samples were prepared to contain different concentrations of red blood cells and nucleated cells to which increasing amounts of Mtb colonies were inoculated and submitted to PCR. RESULTS: Up to 102 CFU/ml of Mtb were detected in the SPU in all methods, except for the Roche extraction/detection method, regardless of the preanalytical sample condition. In BAL samples, medium and high concentrations of cells and high concentrations of red blood cells contributed to a lower Mtb detection, regardless of the extraction method used. In PF, red blood cells were the variable that most interfered with Mtb detection, with better recovery (102 CFU/ml) observed with the Qiagen/Nanogen combination. CONCLUSION: The choice of Mtb extraction and detection method is of fundamental importance for PCR analytical sensitivity, especially when paucibacillary samples and/or samples containing potential PCR inhibitors are analyzed.
Descritores: Derrame Pleural/microbiologia
Escarro/microbiologia
Líquido da Lavagem Broncoalveolar/microbiologia
Reação em Cadeia da Polimerase/métodos
Mycobacterium tuberculosis/isolamento & purificação
-Tuberculose Pleural/microbiologia
DNA Bacteriano/isolamento & purificação
Contagem de Colônia Microbiana
Sensibilidade e Especificidade
Eritrócitos/microbiologia
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-899756
Autor: Núñez, M. Antonieta; Contreras, Karla; Depix, M. Soledad; Geoffroy, Enrique; Villagra, Nicolás; Mellado, Sandra; Salinas, Ana M.
Título: Prevalencia de Bartonella henselae en donantes de sangre y riesgo de transmisión sanguínea en Chile / Prevalence of Bartonella henselae in blood donors and risk of blood transmission in Chile
Fonte: Rev. chil. infectol;34(6):539-543, dic. 2017. graf.
Idioma: es.
Resumo: Resumen Introducción: Bartonella henselae es el agente causal de la enfermedad del arañazo del gato en personas inmunocompetentes y de la angiomatosis bacilar y peliosis hepatis en inmunocomprometidos. En Chile la prevalencia de anticuerpos contra B. henselae en niños y adolescentes sanos es de 13,3%, en personas con riesgo ocupacional 60,5% y en gatos 85,6%. No existen datos publicados respecto de la seroprevalencia en donantes de sangre en nuestro país, por lo que determinar si B. henselae se encuentra presente en la sangre de los donantes al momento de la donación es muy importante, ya que este microorganismo puede sobrevivir hasta 35 días en los eritrocitos almacenados en banco de sangre a 4 °C. Objetivo: Determinar la prevalencia de B. henselae en donantes de sangre. Metodología: Se analizaron 140 muestras de sangre de donantes, para detectar la presencia de B. henselae, utilizando la técnica de la reacción de polimerasa en cadena (RPC). Resultados: Se obtuvo 13,6% de los donantes de sangre con RPC positiva para la B. henselae. La secuencia de los fragmentos amplificados presentó una identidad por sobre 98% con respecto a secuencias de B. henselae de referencia. Conclusión: El riesgo de transmisión sanguínea debiera ser considerado en un país con alta seroprevalencia de infección por B. henselae.

Background: Bartonella henselae is the causal agent of cat scratch disease in immunocompetent persons and bacterial angiomatosis in immunocompromised patients. In Chile, the prevalence of antibodies against B. henselae in healthy children and adolescents is 13.3%, in persons with occupational risk 60.5%, and in cats 85.6%. There are no published data regarding the seroprevalence in blood donors in our country, so determining if B. henselae is present in the blood of donors at the time of donation is very important, since this microorganism can survive up to 35 days in the red blood cells stored in a blood bank at 4 °C. Objective: To determine the prevalence of B. henselae in blood donors. Methodology: 140 donor blood samples were analyzed to detect the presence of B. henselae, using the polymerase chain reaction technique. Results: 13.6% of the blood donors with positive polymerase chain reaction for B. henselae were obtained. The sequence of the amplified fragments showed an identity of over 98% with respect to B. henselae reference sequences. Conclusion: The risk of blood transmission is due to a country with high B. henselae infection.
Descritores: Infecções por Bartonella/sangue
Infecções por Bartonella/epidemiologia
Doadores de Sangue
Bartonella henselae/isolamento & purificação
-Infecções por Bartonella/transmissão
Sangue/microbiologia
Transfusão de Sangue
DNA Bacteriano
Estudos Soroepidemiológicos
Chile/epidemiologia
Reação em Cadeia da Polimerase
Prevalência
Fatores de Risco
Anticorpos Antibacterianos/sangue
Limites: Humanos
Masculino
Feminino
Responsável: CL1.1 - Biblioteca Central


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Id: lil-782105
Autor: Feyisa, Seifu Gizaw; Haeili, Mehri; Zahednamazi, Fatemeh; Mosavari, Nader; Taheri, Mohammad Mohammad; Hamzehloo, Gholamreza; Zamani, Samin; Feizabadi, Mohammad Mehdi.
Título: Molecular characterization of mycobacterium tuberculosis isolates from Tehran, Iran by restriction fragment length polymorphism analysis and spoligotyping
Fonte: Rev. Soc. Bras. Med. Trop;49(2):204-210, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Projeto: Tehran University of Medical Sciences.
Resumo: Abstract: INTRODUCTION Characterization of Mycobacterium tuberculosis (MTB) isolates by DNA fingerprinting has contributed to tuberculosis (TB) control. The aim of this study was to determine the genetic diversity of MTB isolates from Tehran province in Iran. METHODS MTB isolates from 60 Iranian and 10 Afghan TB patients were fingerprinted by standard IS6110-restriction fragment length polymorphism (RFLP) analysis and spoligotyping. RESULTS The copy number of IS6110 ranged from 10-24 per isolate. The isolates were classified into 22 clusters showing ≥ 80% similarity by RFLP analysis. Fourteen multidrug-resistant (MDR) isolates were grouped into 4 IS6110-RFLP clusters, with 10 isolates [71% (95% CI: 45-89%)] in 1 cluster, suggesting a possible epidemiological linkage. Eighteen Iranian isolates showed ≥ 80% similarity with Afghan isolates. There were no strains with identical fingerprints. Spoligotyping of 70 isolates produced 23 distinct patterns. Sixty (85.7%) isolates were grouped into 13 clusters, while the remaining 10 isolates (14.2%) were not clustered. Ural (formerly Haarlem4) (n = 22, 31.4%) was the most common family followed by Central Asian strain (CAS) (n = 18, 25.7%) and T (n = 9, 12.8%) families. Only 1strain was characterized as having the Beijing genotype. Among 60 Iranian and 10 Afghan MTB isolates, 25% (95% CI: 16-37) and 70% (95% CI: 39-89) were categorized as Ural lineage, respectively. CONCLUSIONS A higher prevalence of Ural family MTB isolates among Afghan patients than among Iranian patients suggests the possible transmission of this lineage following the immigration of Afghans to Iran.
Descritores: Tuberculose/microbiologia
Variação Genética/genética
DNA Bacteriano/genética
Técnicas de Tipagem Bacteriana/métodos
Mycobacterium tuberculosis/genética
-Polimorfismo de Fragmento de Restrição
Análise por Conglomerados
Impressões Digitais de DNA
Epidemiologia Molecular
Genótipo
Irã (Geográfico)
Mycobacterium tuberculosis/isolamento & purificação
Limites: Humanos
Responsável: BR1.1 - BIREME


  7 / 627 LILACS  
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Id: lil-785791
Autor: Oliveira, Caio Fernando de; Cavanagh, Jorunn Pauline; Fredheim, Elizabeth G. Aarag; Reiter, Keli Cristine; Rieger, Alexandre; Klingenberg, Claus; d'Azevedo, Pedro Alves; Sollid, Johanna Ericson.
Título: Coagulase-negative staphylococci in Southern Brazil: looking toward its high diversity
Fonte: Rev. Soc. Bras. Med. Trop;49(3):292-299tab, graf.
Idioma: en.
Resumo: Abstract: INTRODUCTION: Coagulase-negative staphylococci (CoNS) are the most prevalent pathogens in nosocomial infections and may serve as a reservoir of mobile genetic elements such as the staphylococcal cassette chromosome mec (SCCmec) encoding methicillin resistance. Molecular characterization of SCCmec types combined with advanced molecular typing techniques may provide essential information for understanding the evolution and epidemiology of CoNS infections. We therefore aimed to investigate the SCCmec distribution, multidrug-resistance (MDR), and biofilm formation in CoNS blood culture isolates from a hospital in Southern Brazil. METHODS: We analyzed 136 CoNS blood culture isolates obtained during 2002-2004 from patients admitted to a tertiary care hospital in Brazil. SCCmec types I to V were determined using multiplex PCR. The clonal relationship of Staphylococcus epidermidis was determined using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Molecular epidemiological data were interpreted along with data on biofilm formation, presence of the icaD gene, and MDR. RESULTS: The most prevalent species were S. epidermidis, Staphylococcus haemolyticus, and Staphylococcus hominis harboring mainly SCCmec types II, III, and V. Overall, the presence of multiple SCCmec was associated with non-MDR, except for S. epidermidis. S. epidermidis isolates showed a high prevalence of icaD, but had low phenotypic biofilm formation. PFGE and MLST revealed high genetic diversity in the S. epidermidis population. CONCLUSIONS: Our results suggest a major shift in SCCmec types within a short period and reveal a different behavior of S. epidermidis with regard to the association between the presence of multiple SCCmec types and MDR profile.
Descritores: Staphylococcus/classificação
Variação Genética/genética
DNA Bacteriano/genética
Cromossomos Bacterianos/genética
-Staphylococcus/enzimologia
Staphylococcus/genética
Eletroforese em Gel de Campo Pulsado
Coagulase/biossíntese
Biofilmes/crescimento & desenvolvimento
Tipagem de Sequências Multilocus
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-792800
Autor: Kobs, Vanessa Cristine; Ferreira, Jéssica Augustini; Bobrowicz, Thaís Alexandra; Ferreira, Leslie Ecker; Deglmann, Roseneide Campos; Westphal, Glauco Adrieno; França, Paulo Henrique Condeixa de.
Título: The role of the genetic elements bla oxa and IS Aba 1 in the Acinetobacter calcoaceticus-Acinetobacter baumannii complex in carbapenem resistance in the hospital setting
Fonte: Rev. Soc. Bras. Med. Trop;49(4):433-440, July-Aug. 2016. tab, graf.
Idioma: en.
Resumo: Abstract: INTRODUCTION: Members of the Acinetobacter genus are key pathogens that cause healthcare-associated infections, and they tend to spread and develop new antibiotic resistance mechanisms. Oxacillinases are primarily responsible for resistance to carbapenem antibiotics. Higher rates of carbapenem hydrolysis might be ascribed to insertion sequences, such as the ISAba1 sequence, near bla OXA genes. The present study examined the occurrence of the genetic elements bla OXA and ISAba1 and their relationship with susceptibility to carbapenems in clinical isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. METHODS: Isolates identified over 6 consecutive years in a general hospital in Joinville, Southern Brazil, were evaluated. The investigation of 5 families of genes encoding oxacillinases and the ISAba1 sequence location relative to bla OXA genes was conducted using polymerase chain reaction. RESULTS: All isolates presented the bla OXA-51-like gene (n = 78), and 91% tested positive for the bla OXA-23-like gene (n = 71). The presence of ISAba1 was exclusively detected in isolates carrying the bla OXA-23-like gene. All isolates in which ISAba1 was found upstream of the bla OXA-23-like gene (n = 69) showed resistance to carbapenems, whereas the only isolate in which ISAba1 was not located near the bla OXA-23-like gene was susceptible to carbapenems. The ISAba1 sequence position of another bla OXA-23-like-positive isolate was inconclusive. The isolates exclusively carrying the bla OXA-51-like gene (n = 7) showed susceptibility to carbapenems. CONCLUSIONS: The presence of the ISAba1 sequence upstream of the bla OXA-23-like gene was strongly associated with carbapenem resistance in isolates of the A. calcoaceticus-A. baumannii complex in the hospital center studied.
Descritores: Proteínas de Bactérias/genética
DNA Bacteriano/genética
Carbapenêmicos/farmacologia
Acinetobacter calcoaceticus/efeitos dos fármacos
Resistência beta-Lactâmica/genética
Acinetobacter baumannii/efeitos dos fármacos
Antibacterianos/farmacologia
-Fenótipo
Proteínas de Bactérias/metabolismo
Brasil
Infecções por Acinetobacter/microbiologia
Reação em Cadeia da Polimerase
Eletroforese em Gel de Campo Pulsado
Acinetobacter calcoaceticus/isolamento & purificação
Acinetobacter calcoaceticus/genética
Acinetobacter baumannii/isolamento & purificação
Acinetobacter baumannii/genética
Genótipo
Limites: Humanos
Responsável: BR1.1 - BIREME


  9 / 627 LILACS  
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Id: lil-792801
Autor: Shahraki-Zahedani, Shahram; Rigi, Shahnaz; Bokaeian, Mohammad; Ansari-Moghaddam, Alireza; Moghadampour, Mehdi.
Título: First report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-producing Klebsiella pneumoniae from Iran
Fonte: Rev. Soc. Bras. Med. Trop;49(4):441-445, July-Aug. 2016. tab, graf.
Idioma: en.
Resumo: Abstract: INTRODUCTION: Extended-spectrum beta-lactamases (ESBLs) are bacterial enzymes capable of hydrolyzing beta-lactams. The aim of this study was to describe the prevalence of TEM- and SHV-type ESBL-producing Klebsiella pneumoniae strains in Zahedan, Southeast Iran. METHODS: A total of 170 non-repetitive K. pneumoniae strains were collected from patients referred to three teaching hospitals of Zahedan. Antibiotic susceptibility testing was determined for 17 antibiotics using the Kirby-Bauer disc diffusion method. The frequency of ESBL-producing strains was calculated, and minimum inhibitory concentrations of ESBL-producing strains were determined for cefotaxime, ceftazidime, ceftriaxone, and cefpodoxime. The presence of bla TEM and bla SHV genes was tested in all ESBL-producing strains using polymerase chain reaction and DNA sequencing. RESULTS: Among the 170 K. pneumoniae clinical isolates, 55 (32.4%) were ESBL producers; 92.7% (n=51) and 72.7% (n=40) of the isolates carried the bla SHV and bla TEM genes, respectively, and 67.3% (n=37) carried both genes. The sequencing results showed that all bla TEM types were bla TEM-1, except for two isolates that were bla TEM-104. The bla SHV types were bla SHV-1, bla SHV-11, bla SHV-12, bla SHV-99, bla SHV-108, and bla SHV-110. CONCLUSIONS: The percentage of bla TEM and bla SHV among ESBL-producing K. pneumoniae isolates from Zahedan is relatively high, indicating the need for further surveillance and consideration in antibiotic use. To the best of our knowledge, this is the first report of TEM-104-, SHV-99-, SHV-108-, and SHV-110-type ESBLs among clinical isolates of K. pneumoniae from Iran, and TEM-1, SHV-1, SHV-11, and SHV-12 appear to be the dominant ESBLs in this region.
Descritores: Proteínas de Bactérias/genética
beta-Lactamases/biossíntese
DNA Bacteriano/genética
Klebsiella pneumoniae/efeitos dos fármacos
Klebsiella pneumoniae/genética
Antibacterianos/farmacologia
-Fenótipo
Infecções por Klebsiella/microbiologia
Análise de Sequência de DNA
Farmacorresistência Bacteriana Múltipla/genética
Testes de Sensibilidade a Antimicrobianos por Disco-Difusão
Genes Bacterianos
Genótipo
Irã (Geográfico)
Limites: Humanos
Responsável: BR1.1 - BIREME


  10 / 627 LILACS  
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Id: biblio-1250191
Autor: Abu Fanas, Salem; Brigi, Carel; Varma, Sudhir Rama; Desai, Vijay; Senok, Abiola; Dsouza, Jovita.
Título: The prevalence of novel periodontal pathogens and bacterial complexes in Stage II generalized periodontitis based on 16S rRNA next generation sequencing
Fonte: J. appl. oral sci;29:e20200787, 2021. tab, graf.
Idioma: en.
Projeto: Ajman University of Science and Technology, Ajman, UAE.
Resumo: Abstract Objective: To define the subgingival microbial profile associated with Stage II generalized periodontitis using next-generation sequencing and to determine the relative abundance of novel periodontal pathogens and bacterial complexes. Methodology: Subgingival biofilm samples were collected from 80 subjects diagnosed with Stage II generalized periodontitis. Bacterial DNA was extracted, and 16S rRNA-based bacterial profiling via next-generation sequencing was carried out. The bacterial composition and diversity of microbial communities based on the age and sex of the patients were analyzed. The bacterial species were organized into groups: bacterial complexes (red, orange, purple, yellow, and green), novel periodontal pathogens, periodontal health-related species, and unclassified periodontal species. The results were analyzed and statistically evaluated. Results: The highest number of bacteria belonged to the phylum Bacteroidetes and Firmicutes. In terms of relative abundance, the orange complex represented 18.99%, novel bacterial species (Fretibacterium spp. and Saccharibacteria spp.) comprised 17.34%, periodontal health-related species accounted for 16.75% and unclassified periodontal species represented (Leptotrichia spp. and Selenomonas spp.) 15.61%. Novel periodontal pathogens had outweighed the periodontal disease-related red complex (5.3%). The one-sample z-test performed was statistically significant at p<0.05. The Beta diversity based on the unweighted UniFrac distance at the species level demonstrated a total variance of 15.77% based on age and 39.19% on sex, which was not statistically significant. Conclusion: The bacterial species corresponding to the disease-related orange complex and novel periodontal pathogens are predominant in Stage II generalized periodontitis.
Descritores: Periodontite
Placa Dentária
-Bactérias/genética
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Prevalência
Sequenciamento de Nucleotídeos em Larga Escala
Limites: Humanos
Adulto
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde