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Pesquisa : D13.444.308.442 [Categoria DeCS]
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Id: biblio-1250488
Autor: Ludwig, Aline; Murer, Laurete; Santos, Helton F. dos; Ludwig, Adriana; Sangioni, Luis Antonio; Vogel, Fernanda S. F.
Título: Molecular detection of Apicomplexa protozoa in tissues from Alouatta guariba clamitans / Detecção molecular de protozoários Apicomplexa em tecidos de Alouatta guariba clamitans
Fonte: Pesqui. vet. bras = Braz. j. vet. res;41:e06717, 2021. tab, ilus.
Idioma: en.
Resumo: The brown howler monkey (Alouatta guariba clamitans) is a primate species widely distributed in South America. Infections by protozoa are common in primates. However, studies on protozoa in primates in Brazil are scarce, so the goal of this study was to investigate DNA from the apicomplexan protozoa Neospora caninum, Sarcocystis spp. and Toxoplasma gondii in tissues of A. guariba clamitans. DNA extraction was performed on tissue samples from the heart, brain, liver, spleen, lung and intestine of six A. guariba clamitans from Santa Maria, Central Region of Rio Grande do Sul, Brazil. Conventional PCR was performed using 18S rRNA gene general primers for Apicomplexa and also specific primers to amplify Neosporaspp. and Toxoplasma gondii DNA. All animals were positive in the 18S PCR and the genetic sequencing confirmed the presence of Sarcocystis spp. DNA in the tissues of four animals belonging to at least two species (S. neurona and S. gigantea) and T. gondii DNA in the other two animals. One positive sample for T. gondii was genotypically characterized as atypical by the restriction fragment length polymorphism technique. N. caninum DNA was not detected in the tested samples. The presence of Apicomplexa protozoan DNA in the tissues of the six animals tested in this study highlights the importance of howler monkeys as maintainers of these pathogens in nature.(AU)

O bugio ruivo (Alouatta guariba clamitans) é uma espécie de primata amplamente distribuída na América do Sul. As infecções por protozoários são comuns em primatas. Entretanto, estudos sobre protozoários em primatas no Brasil são escassos, portanto o objetivo deste estudo foi pesquisar DNA dos protozoários Apicomplexa Neospora caninum, Sarcocystisspp. e Toxoplasma gondii em tecidos de A. guariba clamitans. A extração de DNA foi realizada em amostras de tecido do coração, cérebro, fígado, baço, pulmão e intestino de seis A. guariba clamitans oriundos de Santa Maria, Região Central do Rio Grande do Sul, Brasil. Foi realizada PCR convencional utilizando primers geral do gene 18S rRNA para Apicomplexa e também primers específicos para amplificação de DNA de Neospora spp.e Toxoplasma gondii. Todos os animais foram positivos no PCR geral para Apicomplexa e no sequenciamento genético confirmou-se a presença de DNA de Sarcocystis nos tecidos de quatro animais pertencentes a pelo menos duas espécies (S. neurona e S. gigantea), e DNA de T. gondii foi detectado nos outros dois animais. Uma amostra positiva para T. gondii foi caracterizada genotipicamente como atípico pela técnica de polimorfismo do comprimento do fragmento de restrição. Não foi detectado DNA de N. caninum nas amostras testadas. A presença de DNA de protozoários apicomplexa nos tecidos dos seis animais testados neste estudo destaca a importância dos bugios ruivos como mantenedores desses patógenos na natureza.(AU)
Descritores: Toxoplasma/patogenicidade
Reação em Cadeia da Polimerase
Apicomplexa/patogenicidade
Alouatta/microbiologia
Técnicas de Genotipagem/veterinária
Animais Selvagens/microbiologia
-Infecções por Protozoários/diagnóstico
DNA de Protozoário
Técnicas de Diagnóstico Molecular
Infecções
Limites: Animais
Responsável: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: biblio-1278907
Autor: Melo, Gessica Baptista de; Bosqui, Larissa Rodrigues; Costa, Idessania Nazareth da; Paula, Fabiana Martins de; Gryschek, Ronaldo Cesar Borges.
Título: Current status of research regarding Blastocystis sp, an enigmatic protist, in Brazil
Fonte: Clinics;76:e2489, 2021. tab, graf.
Idioma: en.
Projeto: FAPESP; . CAPES.
Resumo: The present study aimed to evaluate the occurrence of Blastocystis sp. in Brazilian studies over a period of years (2000-2020), as well as point out relevant aspects of this enigmatic organism. We performed a literature search using six sources of international databases. The data were divided into diagnostic by parasitological and molecular techniques, and relevant aspects. After applying the inclusion and exclusion criteria, 52 studies were included in the final analysis. The occurrence of Blastocystis sp. in Brazil ranged from 0.5% to 86.6%, as determined using parasitological techniques. The highest occurrence was in the North (27.3%) and the lowest, in the Midwest region (13.4%). In Brazil, most studies have employed molecular techniques and are concentrated in the Southeast region. The Blastocystis sp. subtype ST3 had the highest average positivity, followed by ST1 and ST2. These findings represent a panorama that reflects the reality of Brazil; thus, we believe that the effectiveness of parasitological diagnosis should be considered with regard to making an appropriate choice of technique for detecting Blastocystis sp. Additionally, we emphasize the importance of further studies in the context of molecular epidemiology with regard to this genus. Blastocystis sp. is not well understood yet, and very little information regarding this genus is available; hence, further research regarding this genus is urgently needed.
Descritores: Infecções por Blastocystis/diagnóstico
Infecções por Blastocystis/epidemiologia
Blastocystis/genética
-Filogenia
Variação Genética
Brasil/epidemiologia
Prevalência
DNA de Protozoário
Fezes
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-782108
Autor: Ferreira, Renata Trotta Barroso; Melandre, Aline Martins; Cabral, Maria Luiza; Branquinho, Maria Regina; Cardarelli-Leite, Paola.
Título: Extraction of trypanosoma cruzi DNA from food: a contribution to the elucidation of acute Chagas disease outbreaks
Fonte: Rev. Soc. Bras. Med. Trop;49(2):190-195, Mar.-Apr. 2016. tab, graf.
Idioma: en.
Resumo: Abstract: INTRODUCTION: Before 2004, the occurrence of acute Chagas disease (ACD) by oral transmission associated with food was scarcely known or investigated. Originally sporadic and circumstantial, ACD occurrences have now become frequent in the Amazon region, with recently related outbreaks spreading to several Brazilian states. These cases are associated with the consumption of açai juice by waste reservoir animals or insect vectors infected with Trypanosoma cruzi in endemic areas. Although guidelines for processing the fruit to minimize contamination through microorganisms and parasites exist, açai-based products must be assessed for quality, for which the demand for appropriate methodologies must be met. METHODS: Dilutions ranging from 5 to 1,000 T. cruzi CL Brener cells were mixed with 2mL of acai juice. Four Extraction of T. cruzi DNA methods were used on the fruit, and the cetyltrimethyl ammonium bromide (CTAB) method was selected according to JRC, 2005. RESULTS: DNA extraction by the CTAB method yielded satisfactory results with regard to purity and concentration for use in PCR. Overall, the methods employed proved that not only extraction efficiency but also high sensitivity in amplification was important. CONCLUSIONS: The method for T. cruzi detection in food is a powerful tool in the epidemiological investigation of outbreaks as it turns epidemiological evidence into supporting data that serve to confirm T. cruzi infection in the foods. It also facilitates food quality control and assessment of good manufacturing practices involving acai-based products.
Descritores: Trypanosoma cruzi/isolamento & purificação
Contaminação de Alimentos
DNA de Protozoário/isolamento & purificação
Parasitologia de Alimentos
Doença de Chagas/transmissão
Euterpe/parasitologia
-Trypanosoma cruzi/genética
Reação em Cadeia da Polimerase
Surtos de Doenças
Doença de Chagas/epidemiologia
Limites: Humanos
Animais
Responsável: BR1.1 - BIREME


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Langoni, Hélio
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Id: lil-798119
Autor: Richini-Pereira, Virgínia Bodelão; Marson, Pâmela Merlo; Silva, Rodrigo Costa da; Langoni, Helio.
Título: Genotyping of toxoplasma gondii and sarcocystis spp. in road-killed wild mammals from the central western region of the state of São Paulo, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;49(5):602-607, Sept.-Oct. 2016. tab.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo.
Resumo: Abstract INTRODUCTION: Road-killed wild animals host zoonotic pathogens such as Toxoplasma gondii, offering a new opportunity for the epidemiological study of these infectious organisms. METHODS This investigation aimed to determine the presence of T. gondii and other apicomplexan parasites in tissue samples of 64 road-killed wild animals, using polymerase chain reaction (PCR). Positive samples were then typed by PCR-restriction fragment length polymorphism (RFLP) using 7 markers: SAG1, 5′-3′SAG2, SAG3, BTUB, c29-6, PK1, and Apico. PCR-RFLP targeting 18S ribosomal RNA (rRNA) genes was also performed on all samples to detect other apicomplexan parasites. RESULTS T. gondii DNA was detected in 16 tissue samples from 8 individual animals, as follows: 1 Cerdocyon thous (crab-eating fox), 1 Didelphis albiventris (white-eared opossum), 1 Lutreolina crassicaudata (lutrine opossum), 2 Myrmecophaga tridactyla (giant anteater), 1 Procyon cancrivorus (crab-eating raccoon), and 2 Sphiggurus spinosus (Paraguay hairy dwarf porcupine). Seven different T. gondii genotypes were identified, 6 of which were novel. Typing by 18S rRNA verified these 16 T. gondii-infected samples, and identified 1 Sarcocystis spp.-infected animal [Dasypus novemcinctus (nine-banded armadillo)]. The amplified T. gondii (GenBank accession No. L37415.1) and Sarcocystis spp. 18S rRNA products were confirmed by sequencing. CONCLUSIONS Our results indicate that T. gondii is commonly present in wild mammals, which act as sources of infection for humans and animals, including other wild species. The approach employed herein proved useful for detecting T. gondii and Sarcocystis spp. in the environment and identifying their natural reservoirs, contributing to our understanding of host-parasite interactions.
Descritores: Toxoplasma/genética
DNA de Protozoário/genética
Sarcocystis/genética
Animais Selvagens/parasitologia
Mamíferos/parasitologia
-Toxoplasma/isolamento & purificação
Brasil
Reação em Cadeia da Polimerase
Sarcocystis/isolamento & purificação
Genótipo
Limites: Animais
Responsável: BR1.1 - BIREME


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Chiari, Egler
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Id: biblio-896996
Autor: Volpato, Fabiana Caroline Zempulski; Sousa, Giovane Rodrigo; D'Ávila, Daniella Alchaar; Galvão, Lúcia Maria da Cunha; Chiari, Egler.
Título: Combined parasitological and molecular-based diagnostic tools improve the detection of Trypanosoma cruzi in single peripheral blood samples from patients with Chagas disease
Fonte: Rev. Soc. Bras. Med. Trop;50(4):506-515, July-Aug. 2017. tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de Minas Gerais; . Conselho Nacional de Desenvolvimento Científico e Tecnológico Chamada Universal.
Resumo: Abstract INTRODUCTION In order to detect Trypanosoma cruzi and determine the genetic profiles of the parasite during the chronic phase of Chagas disease (ChD), parasitological and molecular diagnostic methods were used to assess the blood of 91 patients without specific prior treatment. METHODS Blood samples were collected from 68 patients with cardiac ChD and 23 patients with an indeterminate form of ChD, followed by evaluation using blood culture and polymerase chain reaction. T . cruzi isolates were genotyped using three different genetic markers. RESULTS: Blood culture was positive in 54.9% of all patients, among which 60.3% had the cardiac form of ChD, and 39.1% the indeterminate form of ChD. There were no significant differences in blood culture positivity among patients with cardiac and indeterminate forms. Additionally, patient age and clinical forms did not influence blood culture results. Polymerase chain reaction (PCR) was positive in 98.9% of patients, although comparisons between blood culture and PCR results showed that the two techniques did not agree. Forty-two T . cruzi stocks were isolated, and TcII was detected in 95.2% of isolates. Additionally, one isolate corresponded to TcIII or TcIV, and another corresponded to TcV or TcVI. CONCLUSIONS Blood culture and PCR were both effective for identifying T. cruzi using a single blood sample, and their association did not improve parasite detection. However, we were not able to establish an association between the clinical form of ChD and the genetic profile of the parasite.
Descritores: Trypanosoma cruzi/isolamento & purificação
Trypanosoma cruzi/genética
DNA de Protozoário/genética
Doença de Chagas/diagnóstico
-Reação em Cadeia da Polimerase
Doença Crônica
Sensibilidade e Especificidade
Doença de Chagas/sangue
Hemocultura
Genótipo
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adulto
Idoso
Responsável: BR1.1 - BIREME


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Id: biblio-896966
Autor: Trajano-Silva, Lays Adrianne Mendonça; Pessoa-e-Silva, Rômulo; Gonçalves-de-Albuquerque, Suênia da Cunha; Morais, Rayana Carla Silva de; Costa-Oliveira, Cíntia Nascimento da; Goes, Tayná Correia de; Paiva-Cavalcanti, Milena de.
Título: Standardization and evaluation of a duplex real-time quantitative PCR for the detection of Leishmania infantum DNA: a sample quality control approach
Fonte: Rev. Soc. Bras. Med. Trop;50(3):350-357, May-June 2017. tab, graf.
Idioma: en.
Projeto: Fundação Oswaldo Cruz.
Resumo: Abstract INTRODUCTION: Molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. The present study describes the optimization and evaluation of a duplex real-time quantitative PCR (qPCR) protocol developed for the simultaneous detection of Leishmania infantum DNA and sample quality control. METHODS: After preliminary tests with the newly designed TaqMan® probes for the two targets ( L. infantum and glyceraldehyde 3-phosphate dehydrogenase (G3PD) gene), the duplex qPCR protocol was optimized. For the evaluation of the standardized protocol, human blood samples were tested (n=68) and the results were compared to those obtained by reference diagnostic techniques. Statistical analyses included percentage agreement and the Kappa ( k ) coefficient. RESULTS: The detection limit of L. infantum DNA reached 2x10 2 fg (corresponding to ~1 parasite) per µL of blood (ε: 93.9%). The percentage agreement obtained between the duplex VL qPCR and the reference techniques was individually obtained as follows: molecular: 88.3% ( k =0.666; 95% CI 0.437-0.894, good), and serological: 81.7% ( k =0.411; 95% CI 0.125-0.697, moderate). Between the reference techniques, the percentage agreement was 86.7% ( k =0.586; 95% CI 0.332-0.840, moderate). CONCLUSIONS: The new duplex VL qPCR protocol indicated good potential for the accurate, fast, and reliable detection of L. infantum DNA, when applied as a complement to the classical diagnostic tools already available, especially in health or research reference centers.
Descritores: Controle de Qualidade
DNA de Protozoário/análise
Leishmania infantum/genética
Reação em Cadeia da Polimerase em Tempo Real/normas
Leishmaniose Visceral/diagnóstico
-Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Limites: Humanos
Tipo de Publ: Estudo Comparativo
Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Id: biblio-842817
Autor: Oliveira, Tatiana da Silva Fonseca de; Santos, Barbara Neves dos; Galdino, Tainah Silva; Hasslocher-Moreno, Alejandro Marcel; Bastos, Otilio Machado Pereira; Sousa, Maria Auxiliadora de.
Título: Trypanosoma cruzi I genotype among isolates from patients with chronic Chagas disease followed at the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, Brazil)
Fonte: Rev. Soc. Bras. Med. Trop;50(1):35-43, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT INTRODUCTION: Trypanosoma cruzi is the etiologic agent of Chagas disease in humans, mainly in Latin America. Trypanosome stocks were isolated by hemoculture from patients followed at Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ) and studied using different approaches. METHODS: For species and genotype identification, the stocks were analyzed by parasitological techniques, polymerase chain reaction assays targeted to specific DNA sequences, isoenzyme patterns, besides sequencing of a polymorphic locus of TcSC5D gene (one stock). RESULTS: The isolates presented typical T. cruzi morphology and usually grew well in routine culture media. Metacyclic trypomastigotes were found in cultures or experimentally infected Triatoma infestans. All isolates were pure T. cruzi cultures, presenting typical 330-bp products from kinetoplast DNA minicircles, and 250 or 200-bp amplicons from the mini-exon non-transcribed spacer. Their genetic type assignment was resolved by their isoenzyme profiles. The finding of TcI in one asymptomatic patient from Paraíba was confirmed by the sequencing assay. TcVI was found in two asymptomatic individuals from Bahia and Rio Grande do Sul. TcII was identified in six patients from Pernambuco, Bahia and Minas Gerais, who presented different clinical forms: cardiac (2), digestive with megaesophagus (1), and indeterminate (3). CONCLUSIONS: The main T. cruzi genotypes found in Brazilian chronic patients were identified in this work, including TcI, which is less frequent and usually causes asymptomatic disease, unlike that in other American countries. This study emphasizes the importance of T. cruzi genotyping for possible correlations between the parasite and patient’ responses to therapeutic treatment or disease clinical manifestations.
Descritores: Trypanosoma cruzi/genética
DNA de Protozoário
Doença de Chagas/parasitologia
-Filogenia
Brasil
Reação em Cadeia da Polimerase
Doença Crônica
Genótipo
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adulto
Idoso
Responsável: BR1.1 - BIREME


  8 / 263 LILACS  
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Brazil, Reginaldo Peçanha
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Id: biblio-1041460
Autor: Araujo-Pereira, Thais de; Pita-Pereira, Daniela de; Moreira, Regina Barbosa; Silva-Galdino, Tainah; Duarte, Márcia P. de Oliveira; Brazil, Reginaldo Peçanha; Britto, Constança.
Título: Molecular diagnosis of cutaneous leishmaniasis in an endemic area of Acre State in the Amazonian Region of Brazil
Fonte: Rev. Soc. Bras. Med. Trop;51(3):376-381, Apr.-June 2018. tab, graf.
Idioma: en.
Projeto: CNPq; . Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro; . Armed Forces Health Surveillance Center, Global Emerging Infections Surveillance and Response System.
Resumo: Abstract INTRODUCTION This study proposes to identify the Leishmania species found in the skin lesions of cutaneous leishmaniasis (CL) patients from Brasiléia municipality (Acre). METHODS Skin biopsy imprints or biopsy fragments were assayed via kDNA-PCR/RFLP and FRET-real-time PCR. RESULTS Of individuals with suspected CL, 18 were positive for Leishmania kDNA. Leishmania (Viannia) braziliensis (61.1%) and Leishmania (Viannia) guyanensis (5.5%) were identified in the positive samples. CONCLUSIONS These results are congruent with the previous reports in Acre and Bolivia, revealing L. braziliensis as the most prevalent species. L. guyanensis identification also corroborates with the epidemiology of the disease in the Amazon Basin.
Descritores: Leishmania braziliensis/genética
Leishmaniose Cutânea/diagnóstico
Leishmania guyanensis/genética
-Biópsia
Polimorfismo de Fragmento de Restrição
Brasil/epidemiologia
DNA de Protozoário/genética
Leishmaniose Cutânea/epidemiologia
DNA de Cinetoplasto/genética
Doenças Endêmicas
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Masculino
Feminino
Recém-Nascido
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Adulto Jovem
Responsável: BR1.1 - BIREME


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Id: biblio-957441
Autor: Azevedo, Paulo Hernane Rabelo; Xavier, Marcelo Antônio Pascoal; Silva, Glenda Nicioli da; Costa, Priscilla Almeida da; Carneiro, Cláudia Martins; Brasileiro Filho, Geraldo.
Título: Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi detection
Fonte: Rev. Soc. Bras. Med. Trop;51(4):467-474, July-Aug. 2018. graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. METHODS: We used 1) positive controls (PCs) - 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls - a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases - 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. RESULTS: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. CONCLUSIONS: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease.
Descritores: Trypanosoma cruzi/imunologia
Anticorpos Antiprotozoários/sangue
DNA de Protozoário/análise
Doença de Chagas/diagnóstico
Endocárdio/parasitologia
Anticorpos Monoclonais/sangue
-Biópsia
Imuno-Histoquímica
Estudos de Casos e Controles
Reação em Cadeia da Polimerase
Sensibilidade e Especificidade
Formação de Anticorpos
Limites: Humanos
Tipo de Publ: Estudo de Validação
Responsável: BR1.1 - BIREME


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Coura, José Rodrigues
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Id: biblio-990433
Autor: Miguel, Renata Bortolasse; Albuquerque, Hermano Gomes; Sanchez, Maria Carmen Arroyo; Coura, José Rodrigues; Santos, Simone da Silva; Silva, Sidnei da; Moreira, Carlos José de Carvalho; Suárez-Mutis, Martha Cecilia.
Título: Asymptomatic plasmodium infection in a residual malaria transmission area in the atlantic forest region: implications for elimination
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180537, 2019. tab, graf.
Idioma: en.
Projeto: Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro; . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Abstract INTRODUCTION: Elimination of malaria in areas of interrupted transmission warrants careful case assessment to avoid the reintroduction of this disease. Occasional malaria cases are reported among visitors of the Atlantic Forest area of Brazil, while data on residents of this area are scarce. METHODS: A sectional study was carried out to examine 324 individuals living in a municipality where autochthonous cases were detected. RESULTS: Asymptomatic Plasmodium infections were detected in 2.8% of the individuals by polymerase chain reaction (PCR), with one case of P. falciparum (0.3%), two cases of P. vivax (0.6%), and six cases of P. malariae (1.9%). The thick blood smears were negative in all individuals. Serological tests performed in 314 subjects were reactive in 11.1%, with 3.5% for P. falciparum and 7.7% for P. vivax. A subsample of 42 reactive individuals for any Plasmodium species showed P. malariae in 30.9% of specimens. Individuals who entered the Atlantic Forest region were 2.7 times more likely to exhibit reactive serology for P. vivax compared with individuals who did not enter this region (p<0.05). Children <15 years had a higher chance of reactive serology for P. falciparum and P. vivax than individuals ≥15 years of age (p<0.05). Individuals living in the Paraiso district had a higher chance of reactive serology for P. vivax compared to other districts (p<0.05). No associations were found between sex, past exposure to malaria, or serological response to antibodies of any Plasmodium species. CONCLUSIONS: The implications of these results for the elimination of malaria were discussed.
Descritores: Malária Vivax/diagnóstico
Malária Vivax/transmissão
Malária Falciparum/diagnóstico
Malária Falciparum/transmissão
-Brasil/epidemiologia
Ensaio de Imunoadsorção Enzimática
Reação em Cadeia da Polimerase
Estudos Transversais
DNA de Protozoário/análise
Malária Vivax/epidemiologia
Malária Falciparum/epidemiologia
Infecções Assintomáticas/epidemiologia
Antígenos de Protozoários/imunologia
Limites: Humanos
Masculino
Feminino
Adulto
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME



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