Base de dados : LILACS
Pesquisa : D13.444.308.568 [Categoria DeCS]
Referências encontradas : 459 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 46 ir para página                         

  1 / 459 LILACS  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-839410
Autor: Petito, Guilherme; Carneiro, Megmar Aparecida dos Santos; Santos, Sílvia Helena de Rabello; Silva, Antonio Marcio Teodoro Cordeiro; Alencar, Rita de Cassia; Gontijo, Antonio Paulo; Saddi, Vera Aparecida.
Título: Human papillomavirus in oral cavity and oropharynx carcinomas in the central region of Brazil / Papilomavírus humano (HPV) em carcinomas de cavidade oral e orofaringe na região central do Brasil
Fonte: Braz. j. otorhinolaryngol. (Impr.);83(1):38-44, Jan.-Feb. 2017. tab.
Idioma: en.
Resumo: Abstract Introduction Molecular studies about carcinomas of the oral cavity and oropharynx demonstrate the presence of human papilomavirus genome in these tumors, reinforcing the participation of human papilomavirus in oral carcinogenesis. Objectives This study aimed to determine the prevalence of human papilomavirus and genotype distribution of HPV16 and HPV18 in oral cavity and oropharynx carcinomas, as well as their association with clinical characteristics of the tumors. Methods This is a retrospective study, with clinical data collected from 82 patients. Human papilomavirus detection was conducted on specimens of oral cavity and oropharynx carcinomas included in paraffin blocks. Patients were assisted in a cancer reference center, in the central region of Brazil, between 2005 and 2007. Polymerase chain reaction was used for the detection and genotyping of human papilomavirus. Results Among the patients evaluated, 78% were male. The average age of the group was about 58 years. Risk factors, such as smoking (78%) and alcohol consumption (70.8%) were recorded for the group. HPV DNA was detected in 21 cases (25.6%; 95% confidence interval 16.9–36.6) of which 33.3% were HPV16 and 14.3% were HPV18. The presence of lymph node metastases and registered deaths were less frequent in human papilomavirus positive tumors, suggesting a better prognosis for these cases; however, the differences between the groups were not statistically significant. Conclusion The results obtained in the present study, with respect to the presence of the high-risk HPV16 and HPV18 genotypes, highlight the importance of human papilomavirus vaccination in the control of oral cavity and oropharynx carcinomas.

Resumo Introdução Estudos moleculares sobre carcinomas da cavidade oral e orofaringe demonstram a presença do genoma do papilomavírus humano (HPV) nesses tumores, o que enfatiza a participação do HPV na carcinogênese oral. Objetivos Determinar a prevalência de HPV e a distribuição genotípica de HPV16 e HPV18 nos carcinomas de cavidade oral e orofaringe, bem como sua associação com as características clínicas dos tumores. Método Estudo retrospectivo, com dados clínicos coletados de 82 pacientes. A detecção de HPV foi feita em amostras de carcinomas de cavidade oral e orofaringe incluídos em blocos de parafina. Os pacientes foram atendidos em um centro de referência para tratamento do câncer, na região central do Brasil, entre 2005 e 2007. Foi usada a reação em cadeia de polimerase (PCR) para a detecção e genotipagem do HPV. Resultados Entre os pacientes avaliados, 78% eram homens. A média de idade do grupo era de 58 anos. Fatores de risco como o tabagismo (78%) e consumo de álcool (70,8%) foram registrados para o grupo. HPV DNA foi detectado em 21 casos (25,6%; IC de 95%, 16,9-36,6), dos quais 33,3% eram HPV16 e 14,3% eram HPV18. A presença de metástases em linfonodos e os óbitos registrados foram menos frequentes em tumores positivos para HPV, o que sugere melhor prognóstico para esses casos; contudo, as diferenças entre os grupos não foram estatisticamente significantes. Conclusão Os resultados obtidos no presente estudo, com respeito à presença de genótipos de alto risco de HPV16 e HPV18, destacam a importância da vacinação para HPV no controle dos carcinomas de cavidade oral e orofaringe.
Descritores: Neoplasias Orofaríngeas/virologia
Infecções por Papillomavirus/genética
Infecções por Papillomavirus/virologia
Papillomavirus Humano 16/genética
Papillomavirus Humano 18/genética
Boca/virologia
-Brasil
DNA Viral/genética
Reação em Cadeia da Polimerase
Estudos Retrospectivos
Fatores de Risco
Papillomavirus Humano 18/isolamento & purificação
Genótipo
Limites: Humanos
Masculino
Feminino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


  2 / 459 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: lil-780801
Autor: Zhao, Youyun; Wu, Jianhua; Sun, Lijun; Liu, Guangzhong; Li, Bo; Zheng, Yi; Li, Xiaodong; Tao, Junxiu.
Título: Prevalence of mutations in HBV DNA polymerase gene associated with nucleos(t)ide resistance in treatment-naive patients with Chronic Hepatitis B in Central China
Fonte: Braz. j. infect. dis;20(2):173-178, Mar.-Apr. 2016. tab.
Idioma: en.
Resumo: Abstract Objective There are a lot of disagreements in the studies on hepatitis B virus (HBV) DNA polymerase mutation rate associated with nucleos(t)ide analogues (NAs) in treatment-naive chronic hepatitis B (CHB) patients. This is the first study aimed to investigate the prevalence of spontaneous HBV resistance mutations in Central China. Methods This study included treatment-naive patients with CHB from June 2012 to May 2015 receiving care at the Institute of Liver Disease in Central China. All patients completed a questionnaire covering different aspects, such as family medical history, course of liver disease, medication history, alcohol use, among others. Mutations in HBV DNA polymerase associated with NAs resistance were detected using INNO-LiPA assay. Results 269 patients were infected with HBV genotype B (81.4%), C (17.9%), and both B and C (0.7%). Mutations in HBV DNA polymerase were detected in 24 patients (8.9%) including rtM204I/V (n = 6), rtN236T (n = 5), rtM250V (n = 2), rtL180M (n = 2), rtT184G (n = 1), rtM207I (n = 1), rtS202I (n = 1), rtM204V/I & rtL180M (n = 5), and rtM204I & rtM250V (n = 1). Conclusion Spontaneous HBV resistance mutations in HBV DNA polymerase were found in treatment-naive patients with CHB in Central China. These findings suggest that we should analyze HBV DNA polymerase resistance mutation associated with NAs before giving antiviral therapy such as lamivudine (LAM), adefovir (ADV), and telbivudine (LdT).
Descritores: DNA Viral/genética
Vírus da Hepatite B/genética
Hepatite B Crônica/virologia
Farmacorresistência Viral/genética
DNA Polimerase Dirigida por DNA/genética
Mutação/genética
-Antivirais/uso terapêutico
China
Vírus da Hepatite B/efeitos dos fármacos
Prevalência
Estudos Prospectivos
Hepatite B Crônica/tratamento farmacológico
Genótipo
Limites: Humanos
Masculino
Feminino
Gravidez
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 459 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-828122
Autor: Cayres-Vallinoto, Izaura Maria Vieira; Vallinoto, Antonio Carlos Rosário; Pena, Giselle Priscila dos Anjos; Azevedo, Vânia Nakauth; Machado, Luiz Fernando Almeida; Ishak, Marluísa de Oliveira Guimarães; Ishak, Ricardo.
Título: JC virus/human immunodeficiency virus 1 co-infection in the Brazilian Amazonian region
Fonte: Braz. j. infect. dis;20(4):360-364, July-Aug. 2016. tab, graf.
Idioma: en.
Resumo: Abstract JC virus (JCV) is a member of the Polyomaviridae family and is associated to a severe disease known as progressive multifocal leukoencephalopathy, PML, which is gradually increasing in incidence as an opportunistic infection among AIDS patients. The present study aimed to investigate the occurrence of JCV among HIV-1 carriers including their types and molecular subtypes and the possible association with disease. Urine samples from 66 HIV-1 infected subjects were investigated for the presence of the virus by amplifying VP1 (215 bp) and IG (610 bp) regions using the polymerase chain reaction. JCV was detected in 32% of the samples. The results confirmed the occurrence of type B (subtype Af2); in addition, another polyomavirus, BKV, was also detected in 1.5% of samples of the HIV-1 infected subjects. Apparently, there was no significant difference between mono- (HIV-1 only) and co-infected (HIV-1/JCV) subjects regarding their TCD4+/TCD8+ lymphocyte counts or HIV-1 plasma viral load. Self admitted seizures, hearing and visual loses were not significantly different between the two groups.
Descritores: Leucoencefalopatia Multifocal Progressiva/diagnóstico
Infecções Oportunistas Relacionadas com a AIDS/virologia
Vírus JC/genética
-DNA Viral/urina
Reação em Cadeia da Polimerase
Estudos Transversais
Infecções Oportunistas Relacionadas com a AIDS/diagnóstico
Infecções Oportunistas Relacionadas com a AIDS/urina
Vírus JC/isolamento & purificação
Contagem de Linfócito CD4
Carga Viral
Coinfecção/virologia
Limites: Humanos
Masculino
Feminino
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


  4 / 459 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-839207
Autor: Campos, Karoline Rodrigues; Gonçalves, Maria Gisele; Costa, Nadia Aparecida; Caterino-de-Araujo, Adele.
Título: Comparative performances of serologic and molecular assays for detecting human T lymphotropic virus type 1 and type 2 (HTLV-1 and HTLV-2) in patients infected with human immunodeficiency virus type 1 (HIV-1)
Fonte: Braz. j. infect. dis;21(3):297-305, May-June 2017. tab.
Idioma: en.
Projeto: Ministério da Saúde; . CNPq; . FAPESP; . FAPESP; . CNPq.
Resumo: ABSTRACT The present study evaluated several techniques currently available (commercial kits and in-house assays) for diagnosing human T lymphotropic viruses types 1 and 2 in two groups of patients enrolled at HIV/AIDS specialized care services in São Paulo: Group 1 (G1), n = 1608, 1237 male/371 female, median age 44.3 years old, majority using highly active antiretroviral therapy (HAART); G2, n = 1383, 930 male/453 female, median age of 35.6 years old, majority HAART naïve. Enzyme immunoassays [(EIA) Murex and Gold ELISA] were employed for human T lymphotropic viruses types 1 and 2 screening; Western blotting (WB), INNO-LIA (LIA), real-time PCR pol (qPCR), and nested-PCR-RFLP (tax) were used to confirm infection. Samples were considered human T lymphotropic viruses types 1 and 2 positive when there was reactivity using at least one of the four confirmatory assays. By serological screening, 127/2991 samples were positive or borderline, and human T lymphotropic virus infection was confirmed in 108 samples (three EIA-borderline): 56 human T lymphotropic virus type 1 [G1 (27) + G2 (29)]; 45 human T lymphotropic virus type 2 [G1 (21) + G2 (24)]; one human T lymphotropic virus type 1 + human T lymphotropic virus type 2 (G2); six human T lymphotropic virus [G1 (2) + G2 (4)]. Although there were differences in group characteristics, human T lymphotropic viruses types 1 and 2 prevalence was similar [3.1% (G1) and 4.2% (G2), p = 0.113]. The overall sensitivities of LIA, WB, qPCR, and PCR-RFLP were 97.2%, 82.4%, 68.9%, and 68.4%, respectively, with some differences among groups, likely due to the stage of human T lymphotropic virus infection and/or HAART duration. Indeterminate immunoblotting results were detected in G2, possibly due to the seroconversion period. Negative results in molecular assays could be explained by the use of HAART, the occurrence of defective provirus and/or the low circulating proviral load. In conclusion, when determining the human T lymphotropic virus infection, the findings highlight that there is a need to consider the blood samples with borderline results in screening assays. Of all the tested assays, LIA was the assay of choice for detecting human T lymphotropic virus type 1 and human T lymphotropic virus type 2 in human immunodeficiency virus type 1-infected patients.
Descritores: Infecções por HTLV-I/diagnóstico
Infecções por HTLV-II/diagnóstico
Infecções por HIV/complicações
-DNA Viral/genética
Ensaio de Imunoadsorção Enzimática
Anticorpos Anti-HTLV-I/sangue
Infecções por HTLV-I/complicações
Anticorpos Anti-HTLV-II/sangue
Infecções por HTLV-II/complicações
Western Blotting
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Masculino
Feminino
Adulto
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  5 / 459 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-888889
Autor: Akçay, Emine; Çarhan, Ahmet; Hondur, Gözde; Tufan, Zeliha Koçak; Duru, Necati; Kılıç, Selçuk; Ensari, Ezgi Naz; Uğurlu, Nagihan; Çağıl, Nurullah.
Título: Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study
Fonte: Braz. j. infect. dis;21(4):391-395, July-Aug. 2017. tab.
Idioma: en.
Resumo: Abstract Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients. Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at -80 ºC up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used for control group. Results: A total of 176 samples (conjunctival and pharyngeal samples of 62 patient and 26 healthy subjects) were included. The mean age of 34 (55.7%) male and 27 (44.3%) female patients was 34 ± 17. Twenty five (40.3%) of the patients were receiving antibiotic drops at first visit. The main etiologic agent in conjunctival samples was found to be Adenovirus (46/62, 74.2%) followed by Enterovirus 70 (4/62, 6.4%) and Enterovirus 71 (4/62, 6.4%). Coxsackievirus 16 and 24 were also found in 2 patients (1/62 each, 1.6%). Pharyngeal samples were also positive for Adenovirus (20/62, 32.3%), Enterovirus 70 and 71 (7/62, 11.3% and 5/62, 8.1% respectively), Coxsackievirus 16 and 24 (2/62, 3.2% and 1/61, 1.6%). Conclusions: It is very difficult in viral conjunctivitis to make clinical differentiation caused by different agents because of common clinical signs and symptoms. In routine clinical work, the viral conjunctivitis usually related with Adenovirus. But almost one fourth of the patients' conjunctivitis were not related to Adenovirus, which shows the importance of the laboratory diagnostics. True diagnosis plays an important role on prevention of contamination and unnecessary use of antibiotics in viral conjunctivitis.
Descritores: Faringe/virologia
DNA Viral/isolamento & purificação
Adenoviridae/isolamento & purificação
Conjuntivite Viral/virologia
Enterovirus/isolamento & purificação
-Estudos de Casos e Controles
Adenoviridae/classificação
Adenoviridae/genética
Reação em Cadeia da Polimerase
Doença Aguda
Estudos Prospectivos
Enterovirus/classificação
Enterovirus/genética
Limites: Humanos
Masculino
Feminino
Adulto
Tipo de Publ: Ensaio Clínico Controlado
Responsável: BR1.1 - BIREME


  6 / 459 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Villanova, Marcia Guimaräes
Martinelli, Ana de Lourdes Candolo
Texto completo
Id: biblio-888899
Autor: Chachá, Silvana Gama Florencio; Gomes-Gouvêa, Michele Soares; Malta, Fernanda de Mello; Ferreira, Sandro da Costa; Villanova, Márcia Guimarães; Souza, Fernanda Fernandes; Teixeira, Andreza Correa; Passos, Afonso Dinis da Costa; Pinho, João Renato Rebello; Martinelli, Ana de Lourdes Candolo.
Título: Distribution of HBV subgenotypes in Ribeirão Preto, Southeastern Brazil: a region with history of intense Italian immigration
Fonte: Braz. j. infect. dis;21(4):424-432, July-Aug. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract Hepatitis B virus (HBV) is distributed worldwide, with geographical variations regarding prevalence of the different genotypes. The aim of this study was to determine the HBV genotypes and subgenotypes circulating in Southeast Brazil and compare the genetic sequences found with HBV sequences previously described in the world. Sequences from 166 chronic HBV carriers were analyzed using the fragment constituted by 1306 base pairs comprising surface and polymerase regions of the HBV genome. The sequences obtained were submitted to phylogenetic analysis. HBV subgenotypes A1, A2, D1-D4, F2a, and F4 were found. HBV genotype D was the most frequent, found in 99 patients (58.4%). Within this group, subgenotype D3 was the most prevalent, in 73 patients (42.9%). HBV genotype A was identified in 58 (36%) patients, subgenotype A1, in 48 (29.8%) subjects. Genotype F was identified in 9 (5.4%). According to the phylogenetic analysis, the sequences found were grouped with sequences from Europe, Asia and Middle East (subgenotypes D1, D2, D3) and sequences from Latin America and Africa (subgenotype A1). HBV D3 grouped in different clusters inside D3 clade, several of them with sequences isolated in Italy. We also identified eight families whose relatives were infected with the same HBV subgenotype, most with high similarity between sequences. In conclusion, the distribution of the HBV sequences obtained interweaved with sequences from other continents, corresponding to regions from where many immigrants came to this region, in accordance to the hypothesis that the HBV detected over there were brought during the colonization times.
Descritores: Vírus da Hepatite B/genética
Hepatite B Crônica/virologia
Emigrantes e Imigrantes
-Filogenia
Brasil
DNA Viral/genética
Dados de Sequência Molecular
Análise de Sequência de DNA
Emigração e Imigração
Genótipo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Tipo de Publ: Estudo Comparativo
Responsável: BR1.1 - BIREME


  7 / 459 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-888904
Autor: Silva Souza, Adaliany Cecília da; Souza Marasca, Giórgia de; Kretzmann-Filho, Nélson Alexandre; Dall-Bello, Aline; Kliemann, Dimas Alexandre; Tovo, Cristiane Valle; Veiga, Ana Beatriz Gorini da.
Título: Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
Fonte: Braz. j. infect. dis;21(5):525-529, Sept.-Oct. 2017. tab.
Idioma: en.
Resumo: Abstract Infection by hepatitis B virus (HBV) is a worldwide public health problem. Chronic HBV infection with high viral replication may lead to cirrhosis and/or hepatocellular carcinoma. Mutant HBV strains, such as the HBV A1762T/G1764A double mutant, have been associated with poor prognosis and higher risk of the patient for developing cirrhosis and/or hepatocellular carcinoma. This study analyzed the presence of the HBV A1762T/G1764A double mutant in patients with chronic HBV and its association with clinical parameters such as viral load, aminotransferases, and HBV antigens. A total of 49 patients with chronic hepatitis B were included in the study, and the HBV A1762T/G1764A double mutant strain was detected in four samples (8.16%) by polymerase chain reaction followed by restriction fragment length analysis (PCR-RFLP). The viral load was not significantly different between patients with or without the double mutant strain (p = 0.43). On the other hand, carriers of the HBV A1762T/G1764A double mutant had higher levels of ALT (p = 0.0028), while AST levels did not differ between groups (p = 0.051). In this study, 75% of the samples with the HBV A1762T/G1764A double mutation were HBeAg negative and anti-HBe positive, reflecting seroconversion even though they still displayed high viral loads. Our study has shown that the HBV A1762T/G1764A double mutant strain circulates in Brazilian patients, and is associated with elevated levels of ALT and HBeAg seroconversion.
Descritores: DNA Viral/genética
Vírus da Hepatite B/genética
Hepatite B Crônica/virologia
Antígenos E da Hepatite B/sangue
Mutação/genética
-Brasil
Reação em Cadeia da Polimerase
Estudos Transversais
Análise de Sequência de DNA
Genótipo
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME


  8 / 459 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-984016
Autor: Zhang, Jingmin; Wang, Yafeng; Peng, Youmei; Qin, Chongzhen; Liu, Yixian; Li, Jingjing; Jiang, Jinhua; Zhou, Yubing; Chang, Junbiao; Wang, Qingduan.
Título: Novel fluoronucleoside analog NCC inhibits lamivudine-resistant hepatitis B virus in a hepatocyte model
Fonte: Braz. j. infect. dis;22(6):477-486, Nov.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: This work was supported by grants from the Natural Science Foundation of China; . Young Innovation Foundation of The First Affiliated Hospital of Zhengzhou University of China.
Resumo: ABSTRACT Antiviral drug resistance is the most important factor contributing to treatment failure using nucleos(t)ide analogs such as lamivudine for chronic infection with hepatitis B virus (HBV). Development of a system supporting efficient replication of clinically resistant HBV strains is imperative, and new antiviral drugs are needed urgently to prevent selection of drug-resistant HBV mutants. A novel fluorinated cytidine analog, NCC (N-cyclopropyl-4′-azido-2′-deoxy-2′-fluoro-β-d-cytidine), was recently shown to strongly inhibit human HBV in vitro and in vivo. This study was designed to evaluate the antiviral activity of NCC against lamivudine-resistant HBV. We generated a stable cell line encoding the major pattern of lamivudine-resistant mutations rtL180M/M204V and designated it "HepG2.RL1". Immuno-transmission electron microscopic examination and enzyme-linked immunosorbent assay were used to detect secretion of HBV-specific particles and antigens. Quantification of extracellular DNA and intracellular DNA of HepG2.RL1 cells by quantitative real-time polymerase chain reaction revealed >625-fold and >5556-fold increases in the 50% inhibitory concentration of lamivudine, respectively, compared with that for the wild-type virus. The results showed that NCC inhibited DNA replication and HBeAg production in wild-type or lamivudine-resistant HBV in a dose-dependent manner. In conclusion, screening for antiviral compounds active against lamivudine-resistant HBV can be carried out with relative ease using hepG2.RL1 cells. NCC is a potential antiviral agent against wild-type HBV and clinical lamivudine-resistant HBV and deserves evaluation for the treatment of HBV infection.
Descritores: Antivirais/farmacologia
Replicação Viral/efeitos dos fármacos
Vírus da Hepatite B/efeitos dos fármacos
Lamivudina/farmacologia
Citidina/análogos & derivados
-DNA Viral/química
Testes de Sensibilidade Microbiana
Linhagem Celular
Vírus da Hepatite B/isolamento & purificação
Vírus da Hepatite B/fisiologia
Hepatócitos/virologia
Farmacorresistência Viral/efeitos dos fármacos
Mutação
Limites: Humanos
Feminino
Pessoa de Meia-Idade
Responsável: BR1.1 - BIREME


  9 / 459 LILACS  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1001495
Autor: Haghighi, Malihe Fathi; Seyyedi, Noorossadat; Farhadi, Ali; Zare, Farahnaz; Kasraian, Leila; Dehbidi, Gholam Reza Refiei; Ranjbaran, Reza; Behzad-Behbahani, Abbas.
Título: Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors
Fonte: Braz. j. infect. dis;23(1):22-26, Jan.-Feb. 2019. tab.
Idioma: en.
Projeto: Shiraz University of Medical Sciences Grant Number.
Resumo: ABSTRACT Objectives: To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors. Methods: The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stored at −70 °C for future investigation. DNA was extracted and subjected to simple, sensitive and specific semi-nested PCR as well as QPCR using both general and specific primers for different assays. Results: Of 250 blood samples, 66 (26.4%) were positive for BKV DNA (146-34,514 copies/106 cells). JC DNA was found in 45 (18%) blood samples (65-21,250 copies/106 cells). Co-infection with these viruses were found in 11 (4.4%) out of 250 blood samples. Discussion: Our study provides important data on polyomavirus infection in peripheral blood mononuclear leukocytes in immunocompetent individuals. These data indicate significant differences between the prevalence of BKV and JCV infection in healthy blood donors. The prevalence of BK and JC virus infection is higher in the age range 30-39 years compared to other age ranges.
Descritores: Infecções Tumorais por Vírus/virologia
Doadores de Sangue
Leucócitos Mononucleares/virologia
Vírus BK/isolamento & purificação
Vírus JC/isolamento & purificação
Infecções por Polyomavirus/virologia
-Infecções Tumorais por Vírus/sangue
Infecções Tumorais por Vírus/epidemiologia
DNA Viral/isolamento & purificação
Prevalência
Distribuição por Idade
Vírus BK/genética
Vírus JC/genética
Carga Viral
Infecções por Polyomavirus/sangue
Infecções por Polyomavirus/epidemiologia
Reação em Cadeia da Polimerase em Tempo Real
Irã (Geográfico)/epidemiologia
Limites: Humanos
Masculino
Feminino
Adulto
Pessoa de Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME


  10 / 459 LILACS  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo SciELO Brasil
Texto completo
Id: biblio-1011574
Autor: Rathsam-Pinheiro, Regina Helena; Boa-Sorte, Ney; Grassi, Maria Fernanda Rios; Copello, Úrsula Cury; Rios, Karla Tayrine Silva Guimarães; Araújo, Thessika; Galvão-Castro, Bernardo.
Título: Revisiting Keratoconjunctivitis sicca associated with Human T-Cell Lymphotropic Virus Type 1: prevalence, clinical aspects and proviral load
Fonte: Braz. j. infect. dis;23(2):95-101, Mar.-Apr. 2019. tab.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Fundação de Amparo à Pesquisa do Estado da Bahia.
Resumo: ABSTRACT Background: The prevalence of keratoconjunctivitis sicca (KCS) associated with Human T-Cell Lymphotropic Virus Type 1 (HTLV-1) (HTLV-1/KCS) has been estimated at around 37%, but its clinical manifestations are poorly described. Purpose: To determine the prevalence and associated factors of HTLV-1/KCS in a large cohort of HTLV-1-infected individuals living in Salvador, Brazil. Methods: A cross-sectional study was conducted between June 2004 and September 2017 at the Integrative and Multidisciplinary Center for HTLV in Salvador, Bahia-Brazil. Data from 758 HTLV-1-infected patients was collected. A complete ophthalmologic examination was performed in both eyes. Lacrimal function was evaluated by breakup time, Rose Bengal and Schirmer I Tests. KCS diagnosis was considered in the presence of at least two out of three positive tests. HTLV-1 proviral load Crude and Adjusted Prevalence Rates (PR) with 95% Confidence Intervals (95% CI) were estimated using multivariate Poisson Regression with robust error variance. Results: The overall prevalence of KCS was 31.7%, with higher rates observed in HTLV-1-associated myelopathy/tropical spastic paraparesis patients (crude PR: 1.84; CI95%: 1.50-2.26) even after adjusting for age, sex, time of HTLV-1 diagnosis and schooling (adjusted PR: 1.63; CI95%: 1.31-2.02). Proviral load, low corrected visual acuity, burning and/or pain and itching were all significantly higher in patients with KCS. Conclusion: Burning and/or pain and itching and low corrected visual acuity were the most common alterations of HTLV-1/KCS. High Proviral load was found to be associated with the presence of KCS. It is strongly recommended that HTLV-1 patients undergo periodic ophthalmologic examination to promote the early diagnosis of KCS and prevent the consequences associated with dry eye disease.
Descritores: Vírus Linfotrópico T Tipo 1 Humano/patogenicidade
Ceratoconjuntivite Seca/epidemiologia
Ceratoconjuntivite Seca/virologia
-Fatores Socioeconômicos
Brasil/epidemiologia
DNA Viral
Ensaio de Imunoadsorção Enzimática
Distribuição de Poisson
Fatores Sexuais
Ceratoconjuntivite Seca/patologia
Prevalência
Estudos Transversais
Fatores Etários
Distribuição por Idade
Carga Viral
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Humanos
Masculino
Feminino
Pré-Escolar
Criança
Adolescente
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME



página 1 de 46 ir para página                         
   


Refinar a pesquisa
  Base de dados : Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde