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Id: biblio-974337
Autor: Marinho, Rebeca C; Martins, Gabrielle R; Souza, Kelma C; Sousa, Ana Lídia M; Silva, Sabrina Tainah C; Nobre, Juliana A; Teixeira, Maria F. S.
Título: Duplex nested-PCR for detection of small ruminant lentiviruses
Fonte: Braz. j. microbiol;49(supl.1):83-92, 2018. tab, graf.
Idioma: en.
Projeto: CNPq; . CAPES.
Resumo: Abstract Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosis of SRLV. The technique was designed and tested with the control strains CAEV Co and MVV 1514. Then, field strains were submitted to the same protocol of duplex nested-PCR. Blood samples of sheep and goats were tested with AGID and nested PCR with specific primers for pol, gag and LTR. The AGID results showed low detection capacity of positive animals, while the nested PCR demonstrated a greater capacity of virus detection. Results demonstrated that LTR-PCR was more efficient in detecting positive sheep samples, whereas gag-PCR allowed a good detection of samples of positive goats and positive sheep. In addition, pol-PCR was more efficient with goat samples than for sheep. Duplex nested PCR performed with standard virus samples and field strains demonstrated that the technique is more efficient for the detection of multiple pro-viral DNA sequences. This study demonstrated a successful duplex nested PCR assay allowing a more accurate diagnosis of SRLV.
Descritores: Doenças dos Ovinos/virologia
Doenças das Cabras/virologia
Reação em Cadeia da Polimerase/métodos
Infecções por Lentivirus/veterinária
-Doenças dos Ovinos/diagnóstico
DNA Viral/genética
Cabras
Ovinos
Doenças das Cabras/diagnóstico
Infecções por Lentivirus/diagnóstico
Infecções por Lentivirus/virologia
Primers do DNA/genética
Limites: Animais
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


  2 / 445 LILACS  
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Id: biblio-837975
Autor: Avancini, João; Sanches, José Antonio; Cherubim, Andre Pires Zanata; Pazzini, Renato; Oliveira, Cristina Mendes de; Sumita, Laura Masami; Valente, Neusa Yuriko Sakai; Pannuti, Claudio Sergio; Festa Neto, Cyro.
Título: Angiosarcoma in HIV-negative patients is not associated with HHV-8
Fonte: An. bras. dermatol;91(6):738-741, Nov.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract: BACKGROUND: Angiosarcoma is an aggressive, malignant neoplasm of vascular or lymphatic origin. Herpes virus 8 (HHV-8) is a member of the herpes family with a tropism for endothelial cells and it has been proven to induce vascular neoplasms, such as Kaposi's sarcoma. The role of HHV-8 in the pathogenesis of angiosarcoma has not been well defined. OBJECTIVE: To investigate the relationship between the presence of HHV-8 and angiosarcoma. METHODS: In this study, the team investigated the relationship between the presence of HHV-8, as determined by polymerase chain reaction, and angiosarcoma, using samples from patients with epidemic Kaposi's sarcoma as controls. RESULTS: While all control cases with epidemic Kaposi's sarcoma were positive for HHV-8, none of the angiosarcoma cases was. CONCLUSION: These findings support most previous studies that found no association between HHV-8 and angiosarcoma.
Descritores: Sarcoma de Kaposi/virologia
Neoplasias Cutâneas/virologia
Infecções Oportunistas Relacionadas com a AIDS/virologia
Soronegatividade para HIV
Herpesvirus Humano 8/isolamento & purificação
Hemangiossarcoma/virologia
-Sarcoma de Kaposi/patologia
Neoplasias Cutâneas/patologia
Brasil
DNA Viral
Infecções por HIV/virologia
Reação em Cadeia da Polimerase
Estudos Retrospectivos
Infecções Oportunistas Relacionadas com a AIDS/patologia
Globinas beta/análise
Hemangiossarcoma/patologia
Limites: Seres Humanos
Masculino
Feminino
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


  3 / 445 LILACS  
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Id: biblio-828194
Autor: Rosca, Irina; Petrovici, Anca Roxana; Brebu, Mihai; Stoica, Irina; Minea, Bogdan; Marangoci, Narcisa.
Título: An original method for producing acetaldehyde and diacetyl by yeast fermentation
Fonte: Braz. j. microbiol;47(4):949-954, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Projeto: \"Program of Excellency in the multidisciplinary doctoral and post-doctoral research of chronic diseases\"; . European Social Fund through the Sectoral Operational Program Human Resources Development; . Romanian National Authority for Scientific Research, CNCS - UEFISCDI.
Resumo: Abstract In this study a natural culture medium that mimics the synthetic yeast peptone glucose medium used for yeast fermentations was designed to screen and select yeasts capable of producing high levels of diacetyl and acetaldehyde. The presence of whey powder and sodium citrate in the medium along with manganese and magnesium sulfate enhanced both biomass and aroma development. A total of 52 yeasts strains were cultivated in two different culture media, namely, yeast peptone glucose medium and yeast acetaldehyde-diacetyl medium. The initial screening of the strains was based on the qualitative reaction of the acetaldehyde with Schiff's reagent (violet color) and diacetyl with Brady's reagent (yellow precipitate). The fermented culture media of 10 yeast strains were subsequently analyzed by gas chromatography to quantify the concentration of acetaldehyde and diacetyl synthesized. Total titratable acidity values indicated that a total titratable acidity of 5.5 °SH, implying culture medium at basic pH, was more favorable for the acetaldehyde biosynthesis using strain D15 (Candida lipolytica; 96.05 mg L-1 acetaldehyde) while a total titratable acidity value of 7 °SH facilitated diacetyl flavor synthesis by strain D38 (Candida globosa; 3.58 mg L-1 diacetyl). Importantly, the results presented here suggest that this can be potentially used in the baking industry.
Descritores: Vírus da Hepatite B/genética
Hepatite C/diagnóstico
Hepatite C/virologia
Hepacivirus/genética
Carga Viral
Hepatite B/diagnóstico
Hepatite B/virologia
-DNA Viral
RNA Viral
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  4 / 445 LILACS  
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Id: biblio-828211
Autor: Zauli, Danielle Alves Gomes; Menezes, Carla Lisandre Paula de; Oliveira, Cristiane Lommez de; Mateo, Elvis Cristian Cueva; Ferreira, Alessandro Clayton de Souza.
Título: In-house quantitative real-time PCR for the diagnosis of hepatitis B virus and hepatitis C virus infections
Fonte: Braz. j. microbiol;47(4):987-992, Oct.-Dec. 2016. tab, graf.
Idioma: en.
Resumo: Abstract The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 109 IU/mL of serum, with a coefficient of determination (r2) of 0.99. In sera from patients infected with hepatitis B virus or hepatitis C virus viral loads (19 IU/mL and 1.9 × 109 IU/mL), we quantified viral loads with a detection limit of 1.9 × 102 IU/mL. The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients.
Descritores: Vírus da Hepatite B/genética
Hepatite C/diagnóstico
Hepatite C/virologia
Hepacivirus/genética
Carga Viral
Hepatite B/diagnóstico
Hepatite B/virologia
-DNA Viral
RNA Viral
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Responsável: BR1.1 - BIREME


  5 / 445 LILACS  
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Id: lil-780838
Autor: Souza, Carine Kunzler; Streck, André Felipe; Gonçalves, Karla Ratje; Pinto, Luciane Dubina; Ravazzolo, Ana Paula; Barcellos, David Emílio dos Santos Neves de; Canal, Cláudio Wageck.
Título: Phylogenetic characterization of the first Ungulate tetraparvovirus 2 detected in pigs in Brazil
Fonte: Braz. j. microbiol;47(2):513-517, Apr.-June 2016. tab, graf.
Idioma: en.
Resumo: Abstract Ungulate tetraparvovirus 2 (UTV2) , formerly known as porcine hokovirus due to its discovery in Hong Kong, is closely related to a Primate tetraparvovirus (human PARV-4) and Ungulate tetraparvovirus 1 (bovine hokovirus). Until now, UTV2 was detected in European, Asian and North American countries, but its occurrence in Latin America is still unknown. This study describes the first report of UTV2 in Brazil, as well as its phylogenetic characterization. Tissue samples (lymph node, lung, liver, spleen and kidney) of 240 piglets from eight different herds (30 animals each herd) were processed for DNA extraction. UTV2 DNA was detected by PCR and the entire VP1/VP2 gene was sequenced for phylogenetic analysis. All pigs from this study displayed postweaning multisystemic wasting syndrome (PMWS). UTV2 was detected in 55.3% of the samples distributed in the variety of porcine tissues investigated, as well as detected in almost all herds, with one exception. The phylogenetic analysis demonstrated that Brazilian UTV2 sequences were more closely related to sequences from Europe and United States.
Descritores: Filogenia
Doenças dos Suínos/virologia
Infecções por Parvoviridae/veterinária
Parvovirinae/isolamento & purificação
Parvovirinae/classificação
-Suínos
Brasil
DNA Viral/genética
Infecções por Parvoviridae/virologia
Parvovirinae/genética
Limites: Animais
Responsável: BR1.1 - BIREME


  6 / 445 LILACS  
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Lemos, Marcílio Figueiredo
Moreira, Regina Celia
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Id: lil-772614
Autor: Santos, Ana Paula de Torres; Levi, José Eduardo; Lemos, Marcilio Figueiredo; Calux, Samira Julien; Oba, Isabel Takano; Moreira, Regina Célia.
Título: An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA
Fonte: Mem. Inst. Oswaldo Cruz;111(2):134-140, Feb. 2016. tab, graf.
Idioma: en.
Projeto: FAPESP.
Resumo: This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fifty-two samples were from patients who were human immunodeficiency virus and hepatitis C virus positive, but HBV negative. Genotypes were characterised, and the viral load was measure in each sample. The in-house rtPCR showed an excellent success rate compared with commercial tests; inter-assay and intra-assay coefficients correlated with commercial tests (r = 0.96 and r = 0.913, p < 0.001) and the in-house test showed no genotype-dependent differences in detection and quantification rates. The in-house assay tested in this study could be used for screening and quantifying HBV DNA in order to monitor patients during therapy.
Descritores: DNA Viral/isolamento & purificação
Técnicas de Genotipagem/normas
Vírus da Hepatite B/isolamento & purificação
Hepatite B Crônica/diagnóstico
Técnicas de Diagnóstico Molecular
Reação em Cadeia da Polimerase em Tempo Real/normas
-Primers do DNA/normas
Estudos de Avaliação como Assunto
Genótipo
Soropositividade para HIV/sangue
Soropositividade para HIV/diagnóstico
Vírus da Hepatite B/genética
Hepatite B Crônica/sangue
Hepatite C/sangue
Hepatite C/diagnóstico
Invenções/normas
Técnicas de Diagnóstico Molecular/instrumentação
Técnicas de Diagnóstico Molecular/métodos
Sensibilidade e Especificidade
Carga Viral
Limites: Seres Humanos
Masculino
Feminino
Lactente
Pré-Escolar
Criança
Adolescente
Adulto
Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Tipo de Publ: Research Support, Non-U.S. Gov't
Estudo Comparativo
Responsável: BR1.1 - BIREME


  7 / 445 LILACS  
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Id: lil-734667
Autor: Hoffstetter, René; Andana, Alejandra; Guzmán, Pablo; Ili, Carmen G; Retamal, Javier; Mora, Bárbara; Roa, Juan C; Sánchez, Raúl.
Título: Frecuencia de virus papiloma humano en tumores no ginecológicos de la Región de la Araucanía, Chile / Human papilloma virus frequency in non-gynecological tumors in the Araucanía Region, Chile
Fonte: Int. j. morphol;32(4):1254-1260, Dec. 2014. ilus.
Idioma: es.
Projeto: MEC; . CORFO; . DIUFRO; . CORFO-CEGIN; . Scientific and Technological Bioresource Nucleus; . FONDECYT.
Resumo: El Virus Papiloma Humano (HPV por sus siglas en inglés) es una de las infecciones de transmisión sexual más frecuentes del mundo y se encuentra presente en la mayoría de los cánceres de cuello uterino. Se ha descrito su presencia en otros tipos de cáncer no ginecológicos como lo son esófago y próstata. Sin embargo, las frecuencias de HPV descritas hasta el momento para estos tipos de cáncer son muy variables, y no hay artículos donde se muestren la presencia de HPV en estas neoplasias en Chile. El objetivo de este estudio fue determinar la frecuencia de HPV en muestras de biopsias de tumores no ginecológicos y tejido inflamatorio de pacientes de la región de La Araucanía. Se extrajo DNA desde un total de 47 biopsias de pacientes con esofagitis, 25 con carcinoma escamoso esofágico, 20 con hiperplasia nodular de la próstata y 39 con adenocarcinoma prostático. Estas fueron analizadas por PCR de la región L1 del virus y posterior genotipificación por reverse line blot. Se detectó HPV en el 53,2% de las muestras de esofagitis, 48% en muestras de carcinoma escamoso esofágico, 15% en hiperplasia nodular de la próstata y un 15,4% en los casos de adenocarcinoma prostático. Siendo los más frecuentes los genotipos de HPV 16 y 18, ya sea en infecciones simples o junto con otros genotipos, en lesiones preneoplásicas y neoplásicas de los tejidos estudiados. Existe una alta frecuencia de infección por HPV en biopsias de esofagitis y tejido inflamatorio esofágico de pacientes de la región de la Araucanía. En los casos de adenocarcinoma prostático e hiperplasia nodular de la próstata se observa una baja frecuencia de HPV.

Human Papilloma Virus (HPV) is the most common sexually transmitted disease in the world and it is present in practically all cervical cancers. Its presence was described in other types of non-gynecologic cancer such as esophageal and prostate. However, HPV frequency described for these cancers is highly variable, and there are no articles describing the presence of HPV in these tumors in Chile. To determine HPV frequency in samples from biopsies of non-gynecological tumors and inflammatory tissue from patients in the Araucanía region, DNA was extracted from a total of 47 biopsies from patients with esophagitis, 25 with esophageal squamous cell carcinoma, 20 with prostate nodular hyperplasia and 39 with prostate adenocarcinoma. These were analyzed by PCR of HPV L1 region and subsequent genotyping by reverse line blot. HPV was detected in 53.2% of esophagitis samples, 48% in esophageal squamous cell carcinoma, 15% in prostatitis and 15.4% in cases of prostatic adenocarcinoma. The most frequent HPV genotypes were 16 and 18, either single or in combination with other genotype infections, in inflammatory tissue and neoplastic lesions. In patients of the Araucanía region, there is a high rate of HPV infection in biopsies obtained in esophagitis and esophageal inflammatory tissue. In cases of prostatic adenocarcinoma and prostate nodular hyperplasia a low rate of HPV was observed.
Descritores: Neoplasias da Próstata/virologia
Neoplasias Esofágicas/virologia
Infecções por Papillomavirus/complicações
-Papillomaviridae/isolamento & purificação
Papillomaviridae/genética
Hiperplasia Prostática/virologia
DNA Viral
Carcinoma de Células Escamosas/virologia
Adenocarcinoma/virologia
Chile
Reação em Cadeia da Polimerase
Infecções por Papillomavirus/virologia
Esofagite/virologia
Genótipo
Limites: Seres Humanos
Masculino
Feminino
Meia-Idade
Idoso
Responsável: CL1.1 - Biblioteca Central


  8 / 445 LILACS  
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Id: biblio-984761
Autor: Cataneo, Allan Henrique Depieri; Kuczera, Diogo; Mosimann, Ana Luiza Pamplona; Silva, Emanuele Guimarães; Ferreira, Álvaro Gil Araújo; Marques, João Trindade; Wowk, Pryscilla Fanini; Santos, Claudia Nunes Duarte dos; Bordignon, Juliano.
Título: Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus
Fonte: Mem. Inst. Oswaldo Cruz;114:e180432, 2019. tab, graf.
Idioma: en.
Projeto: Ministério da Saúde; . Fundação Araucária; . Fundação Araucária; . SESA-PR; . CNPq; . MS-Decit; . PPSUS; . JTM; . CNDS; . JB; . CNPq; . AHDC; . DK; . CNDS; . JB.
Resumo: BACKGROUND The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral strains for their studies. C6/36 cells derived from Aedes albopictus larvae are commonly used for arbovirus isolation from clinical samples and for the preparation of viral stocks. OBJECTIVES Here, we report the contamination of two widely used ZIKV strains by Brevidensovirus, here designated as mosquito densovirus (MDV). METHODS Molecular and immunological techniques were used to analyse the MDV contamination of ZIKV stocks. Also, virus passages in mammalian cell line and infecting susceptible mice were used to MDV clearance from ZIKV stocks. FINDINGS MDV contamination was confirmed by molecular and immunological techniques and likely originated from C6/36 cultures commonly used to grow viral stocks. We applied two protocols that successfully eliminated MDV contamination from ZIKV stocks, and these protocols can be widely applied in the field. As MDV does not infect vertebrate cells, we performed serial passages of contaminated stocks using a mammalian cell line and infecting susceptible mice prior to re-isolating ZIKV from the animals' blood serum. MDV elimination was confirmed with immunostaining, polymerase chain reaction (PCR), and analysis of the mosquitoes that were allowed to feed on the infected mice. MAIN CONCLUSIONS Since the putative impact of viral contaminants in ZIKV strains generally used for research purposes is unknown, researchers working in the field must be aware of potential contaminants and test viral stocks to certify sample purity.
Descritores: Cultura de Vírus
Bancos de Espécimes Biológicos
Zika virus
-DNA Viral
Imunofluorescência
Densovirus/genética
Camundongos
Limites: Seres Humanos
Animais
Responsável: BR1.1 - BIREME


  9 / 445 LILACS  
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Id: biblio-894837
Autor: Oliveira, Gisele R de; Siqueira, Juliana D; Finger-Jardim, Fabiana; Vieira, Valdimara C; Silva, Ronald L; Gonçalves, Carla V; Soares, Esmeralda A; Martinez, Ana Maria Barral de; Soares, Marcelo A.
Título: Characterisation of complete high- and low-risk human papillomavirus genomes isolated from cervical specimens in southern Brazil
Fonte: Mem. Inst. Oswaldo Cruz;112(10):728-731, Oct. 2017. tab.
Idioma: en.
Projeto: FAPERJ; . CNPq; . FAPERGS.
Resumo: The classification of human papillomavirus (HPV) intratypic lineages by complete genome sequencing is a determinant in understanding biological differences in association with this disease. In this work, we have characterised complete HPV genomes from southern Brazil. Fifteen cervicovaginal Pap smear negative samples previously categorised as HPV-positive were sequenced using ultradeep sequencing, and 18 complete genomes from 13 different HPV types were assembled. Phylogenetic and genetic distance analyses were performed to classify the HPV genomes into lineages and sublineages. This is the first report describing the distribution of HPV intratype lineages of high and low oncogenic risk in asymptomatic women from southern Brazil.
Descritores: Papillomaviridae
Papillomaviridae/genética
Esfregaço Vaginal
DNA Viral
Doenças do Colo do Útero/virologia
Genoma Viral
Infecções por Papillomavirus/virologia
-Fatores de Risco
Limites: Seres Humanos
Feminino
Adulto
Responsável: BR1.1 - BIREME


  10 / 445 LILACS  
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Id: biblio-841811
Autor: de Oliveira, Gisele R; Vieira, Valdimara C; Ávila, Emiliana C; Finger-Jardim, Fabiana; Caldeira, Thaís DM; Gatti, Fabiane AA; Gonçalves, Carla V; Oliveira, Sandro G; da Hora, Vanusa P; Soares, Marcelo A; de Martinez, Ana MB.
Título: Human papillomavirus type distribution and HPV16 intratype diversity in southern Brazil in women with and without cervical lesions
Fonte: Mem. Inst. Oswaldo Cruz;112(7):492-498, July 2017. tab.
Idioma: en.
Projeto: CAPES; . CNPq; . FAPERJ.
Resumo: BACKGROUND Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer. OBJECTIVES This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in southern Brazil in women with normal cytology or intraepithelial lesions. METHODS HPV typing was determined by L1 gene sequencing. To identify HPV16 variants, the LCR and E6 regions were sequenced, and characteristic single nucleotide variants were identified. FINDINGS A total of 445 samples were studied, with 355 from cervical scrapes and 90 from cervical biopsies. HPV was detected in 24% and 91% of these samples, respectively. The most prevalent HPV types observed were 16 (cervical, 24%; biopsies, 57%) and 58 (cervical, 12%; biopsies, 12%). Seventy-five percent of the HPV16-positive samples were classified into lineages, with 88% defined as lineage A, 10% as lineage D, and 2% as lineage B. MAIN CONCLUSIONS This study identified a high frequency of European and North American HPV16 lineages, consistent with the genetic background of the human population in southern Brazil.
Descritores: Variação Genética/genética
DNA Viral/genética
Neoplasias do Colo do Útero/virologia
Infecções por Papillomavirus/virologia
Papillomavirus Humano 16/genética
-Fatores Socioeconômicos
Brasil
Displasia do Colo do Útero
Reação em Cadeia da Polimerase
Estudos Transversais
Limites: Seres Humanos
Feminino
Adulto
Responsável: BR1.1 - BIREME



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