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Id: biblio-953185
Autor: Borges, Bruna Emanuelle Sanches; Brito, Elza Baia de; Fuzii, Hellen Thais; Baltazar, Cláudia Simone; Sá, Aline Barreto; Silva, Camile Irene Mota da; Santos, Gleyce de Fátima Silva; Pinheiro, Maria da Conceição Nascimento.
Título: Human papillomavirus infection and cervical cancer precursor lesions in women living by Amazon rivers: investigation of relations with markers of oxidative stress / Infecção por papilomavírus humano e lesões precursoras do câncer cervicouterino em Ribeirinhas da Amazônia: avaliação da relação com marcadores de estresse oxidativo
Fonte: Einstein (Säo Paulo);16(3):eAO4190, 2018. tab.
Idioma: en.
Resumo: ABSTRACT Objective To investigate the relation between oxidative stress markers, human papillomavirus infection and cervical cancer precursor lesions. Methods The study comprised women aged 14 to 60 years living in communities located by Amazon rivers in the state of Pará (Itaituba, Limoeiro do Ajuru and Bragança, 126, 68 and 43 women respectively). Papanicolau smears and polymerase chain reaction tests for human papillomavirus DNA detection were performed. Blood samples were collected to test malondialdehyde, total and oxidized glutathione levels. Results Malondialdehyde, total and oxidized glutathione concentrations did not differ significantly (p>0.05) between women with and without low-grade squamous intraepithelial lesions across communities. Malondialdehyde levels (8.02nmols/mL) were almost five times higher in human papillomavirus-positive compared to human papillomavirus-negative women (1.70nmols/mL) living in Itaituba (statistically significant difference; p<0.05). Malondialdehyde levels did not differ significantly (p>0.05) between human papillomavirus-positive and human papillomavirus-negative women living in remaining communities. Significant (p<0.05) differences in total glutathione levels between human papillomavirus-positive and human papillomavirus-negative women (8.20μg/mL and 1.47μg/mL, respectively) were limited to those living in Bragança. Conclusion Malondialdehyde and total glutathione levels were significantly associated with human papillomavirus infection. However, lack of similar associations with squamous lesions suggest oxidative stress alone does not explain correlations with cervical carcinogenesis. Other factors may therefore be involved.

RESUMO Objetivo Avaliar a relação de marcadores de estresse oxidativo com a infecção pelo papilomavírus humano e as lesões precursoras do câncer cervical. Métodos Foram incluídas mulheres ribeirinhas da Amazônia (a saber: 126 do município de Itaituba, 68 de Limoeiro do Ajuru e 43 de Bragança), todas do Estado do Pará, de 14 a 60 anos. Foram realizados testes Papanicolau e de reação em cadeia de polimerase para a detecção de DNA do papilomavírus humano. Coleta de amostras de sangue foi realizada para a avaliação do malondialdeído e a determinação da glutationa total e oxidada. Resultados As concentrações oxidativas do malondialdeído e os teores de glutationa total e oxidada não apresentaram diferença significativa (p>0,05) nas mulheres com e sem lesão intraepitelial escamosa de baixo grau, em todas as comunidades pesquisadas. Mulheres com papilomavírus humano em Itaituba apresentaram níveis de malondialdeído (8,02nmols/mL) quase cinco vezes maior quando relacionadas àquelas sem o HPV (1,70nmols/mL), com diferença estatística significativa (p<0,05). As outras comunidades não mostraram diferença estatística significativa entre as concentrações de malondialdeído nas mulheres com e sem papilomavírus humano (p>0,05). Os teores de glutationa total mostraram diferença significativa (p<0,05) somente em Bragança naquelas com (8,20µg/mL) e sem a infecção pelo papilomavírus humano (1,47µg/mL). Conclusão Houve associação significativa da resposta oxidativa do malondialdeído e da glutationa total com a infecção pelo papilomavírus humano, porém não houve diferença quando associada à lesão escamosa, sugerindo que o estresse oxidativo isoladamente não explica a relação com a carcinogênese do colo uterino, que deve ser influenciada ainda por outros fatores.
Descritores: Neoplasias do Colo do Útero/sangue
Estresse Oxidativo/fisiologia
Dissulfeto de Glutationa/sangue
Infecções por Papillomavirus/sangue
Lesões Intraepiteliais Escamosas Cervicais/sangue
Glutationa Peroxidase/sangue
Malondialdeído/sangue
-Papillomaviridae/isolamento & purificação
Valores de Referência
Índice de Gravidade de Doença
Brasil
DNA Viral
Biomarcadores Tumorais/sangue
Neoplasias do Colo do Útero/virologia
Reação em Cadeia da Polimerase
Estudos Transversais
Estatísticas não Paramétricas
Infecções por Papillomavirus/complicações
Rios
Teste de Papanicolaou
Lesões Intraepiteliais Escamosas Cervicais/virologia
Pessoa de Meia-Idade
Limites: Humanos
Feminino
Adolescente
Adulto
Adulto Jovem
Tipo de Publ: Estudo Observacional
Responsável: BR1.1 - BIREME


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Id: biblio-960051
Autor: Rodríguez, Laura; Otero, William; Grosso, Fabio.
Título: Cáncer metastásico con primario desconocido. Una revisión / A review of metastatic cancer with unknown primary cancer
Fonte: Rev. colomb. gastroenterol;33(2):134-144, abr.-jun. 2018. tab, graf.
Idioma: es.
Resumo: Resumen El tumor metastásico no siempre tiene un origen evidente, hasta en un tercio de los casos nunca se encuentra el tumor primario. Este artículo es una guía de los avances más recientes para mejorar el enfoque diagnóstico y el manejo del paciente con este tumor fatal y frecuente. El objetivo de este artículo, además de ser una guía, es ayudar a evitar errores comunes y graves. Uno de los errores más importantes es no tener en cuenta el papel fundamental de la confirmación histológica, pues esta puede evitar investigaciones innecesarias. En el artículo también se detallan los componentes de la evaluación estándar, la clasificación según su pronóstico y las indicaciones de la evaluación secundaria, que incluye las indicaciones de la endoscopia alta y baja, los marcadores tumorales, la tomografía por emisión de positrones (TEP), el papel que ocupa el perfil genético, la epigenética y el ácido desoxirribonucleico (ADN) viral. Adicionalmente, se indica el momento en que se debe detener la investigación. Recientemente, el tratamiento se ha modificado, lo que parece cambiar la historia de estos pacientes y de sus contrapartes con primario conocido.

Abstract Metastatic tumors do not always have obvious origins: in one third of these cases, the primary tumor is never found. This article is a guide to the most recent advances in diagnostic approaches and patient management of these fatal and frequent tumors. An additional objective of this article is to help avoid common and serious errors. One of the most important errors is not taking the fundamental role of histological confirmation into account since it can avoid unnecessary investigations. The article also details the components of a standard evaluation, classification according to prognosis and indications for a secondary evaluation. These include indications for upper and lower endoscopy, tumor markers, positron emission tomography, and the roles of genetic profiling, epigenetics and viral DNA. It also indicates the moment at which an investigation should be stopped. Recently, treatment has changed, and these changes seems to have changed the history of these patients and their counterparts with known primary tumors.
Descritores: DNA Viral
Biomarcadores Tumorais
Tomografia por Emissão de Pósitrons
Neoplasias
-Pacientes
Prognóstico
Diagnóstico
Endoscopia
Limites: Humanos
Tipo de Publ: Revisão
Responsável: CO354 - Sociedad Colombiana de Gastroenterología


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Id: biblio-840810
Autor: Aydin, Merve; Bozkurt, Aliseydi; Cikman, Aytekin; Gulhan, Baris; Karabakan, Mehmet; Gokce, Aysun; Alper, Murat; Kara, Murat.
Título: Lack of evidence of HPV etiology of prostate cancer following radical surgery and higher frequency of the Arg/Pro genotype in turkish men with prostate cancer
Fonte: Int. braz. j. urol;43(1):36-46, Jan.-Feb. 2017. tab, graf.
Idioma: en.
Projeto: Erzincan University.
Resumo: ABSTRACT Objectives The aim of this study was to assess the possible role of HPV in the development of prostate cancer (PCa) and investigate the distribution of the p53 codon 72 polymorphism in PCa in a Turkish population. Materials and methods A total of 96 tissues, which had been obtained using a radical surgery method, formalin-fixed and parafin-embedded, were used in this study. The study group consisted of 60 PCa tissues (open radical prostatectomy) and the control group contained 36 benign prostatic hyperplasia tissues (BPH) (transvesical open prostatectomy). The presence of HPV and the p53 codon 72 polymorphism was investigated in both groups using real-time PCR and pyrosequencing. Results The results of the real-time PCR showed no HPV DNA in any of the 36 BPH tissue samples. HPV-DNA was positive in only 1 of the 60 PCa samples (1.7%). The HPV type of this sample was identified as HPV-57. The distribution of the three genotypes, Arg/Arg, Arg/Pro and Pro/Pro was found to be 45.6, 45.6, and 8.8% in the PCa group and 57.1%, 34.3% and 8.6% in the control group, respectively. Compared with the control group, patients with PCa had a higher frequency of the Arg/Pro genotype and Proline allele (odds ratio (OR)=1.67, 95% confidence interval (CI)=0.68-4.09, p=0.044; OR=1.13, 95% CI=0.76-1.68, p=0.021, respectively). Conclusions The results of the study do not support the hyphothesis that prostate cancer is associated with HPV infection but indicated that Proline allele can be a risk factor in the development of PCa in the Turkish population.
Descritores: Papillomaviridae/isolamento & purificação
Polimorfismo Genético
Neoplasias da Próstata/genética
Neoplasias da Próstata/virologia
Proteína Supressora de Tumor p53/genética
Infecções por Papillomavirus/complicações
-Prostatectomia
Hiperplasia Prostática/genética
Hiperplasia Prostática/patologia
Hiperplasia Prostática/virologia
Neoplasias da Próstata/cirurgia
Neoplasias da Próstata/patologia
Turquia
Códon/genética
DNA Viral
Prolina/genética
Estudos Retrospectivos
Fatores de Risco
Inclusão em Parafina
Estudos de Associação Genética
Gradação de Tumores
Técnicas de Genotipagem
Reação em Cadeia da Polimerase em Tempo Real
Genótipo
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


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Almeida, Paulo Roberto Carvalho de
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Id: biblio-974922
Autor: Cavalcante, José Roosevelt; Pinheiro, Luiz Gonzaga Porto; Almeida, Paulo Roberto Carvalho de; Ferreira, Márcia Valéria Pitombeira; Cruz, Gizele Almada; Campelo, Thales Alves; Silva, Caroliny Soares; Lima, Luana Nepomuceno Gondim Costa; Oliveira, Bruno Masato Kitagawa de; Lima, Lara Mulato; Feitosa, Laura Magda Costa; Pinheiro, Agostinho Câmara; Frota, Cristiane Cunha.
Título: Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence
Fonte: Clinics;73:e465, 2018. tab, graf.
Idioma: en.
Resumo: OBJECTIVE: The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil. METHOD: One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region. RESULTS: Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples. CONCLUSIONS: The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.
Descritores: Neoplasias da Mama/virologia
DNA Viral/genética
Infecções por Papillomavirus/genética
-Estudos de Casos e Controles
Reação em Cadeia da Polimerase
Estudos Transversais
Genótipo
Limites: Humanos
Feminino
Adulto
Pessoa de Meia-Idade
Idoso
Idoso de 80 Anos ou mais
Responsável: BR1.1 - BIREME


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Id: biblio-899767
Autor: Flores, Carlos; González, Erika; Verna, Andrea; Peralta, Andrea; Madariaga, Carolina; Odeón, Anselmo; Cantón, Germán.
Título: Virus orf en humanos, confirmación molecular de un caso clínico en Chile / Orf virus in human, confirmation in case report from Chile
Fonte: Rev. chil. infectol;34(6):607-609, dic. 2017. graf.
Idioma: es.
Resumo: Resumen La infección por el virus orf, también conocida como ectima contagioso, es reconocida una zoonosis ocupacional. Se diagnostica por lesiones cutáneas que evolu cionan rápidamente desde máculas a pápulas, vesículas y pústulas. Se presenta el caso clínico de una estudiante de medicina veterinaria que había tenido contacto con caprinos, clínicamente sanos y sin lesiones aparentes, hacía 19 días. Presentó dos lesiones vesiculares que coalescieron hasta formar una lesión de mayor tamaño rodeada por un halo eritematoso. Las lesiones fueron compatibles con la presentación clásica de las producidas por el virus orf en humanos. Se confirmó la presencia del virus orf mediante una RPC anidada del tejido de biopsia. Es uno de los primeros casos confirmados mediante técnicas moleculares en seres humanos en Chile.

Infection with the orf virus, also known as contagious ecthyma, is recognized as an occupational zoonosis worldwide. It is diagnosed by cutaneous lesions that progress rapidly from macules to papules, vesicles and pustules. The clinical case of a student of veterinary medicine who had had contact with goats, clinically healthy and without apparent lesions, which occured 19 days ago, is reported. She presented two vesicular lesions that coalesced to form a larger lesion surrounded by an erythematous halo. The lesions were compatible with the classical presentation of those produced by the orf virus in humans. The presence of the orf virus was confirmed by a nested PCR from biopsy tissue. It is one of the first cases confirmed by molecular techniques in humans in Chile.
Descritores: Vírus do Orf/isolamento & purificação
Ectima Contagioso/patologia
-Vírus do Orf/patogenicidade
Biópsia
DNA Viral
Cabras
Doenças das Cabras/virologia
Chile
Reação em Cadeia da Polimerase
Dermatopatias Virais/patologia
Limites: Humanos
Animais
Feminino
Adulto Jovem
Tipo de Publ: Relatos de Casos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1055886
Autor: Santos, Helen Nazareth Veloso dos; Ferracioli-Oda, Eduardo; Barbosa, Thaisa Silveira; Otani, Camila Sayuri Vicentini; Tanaka, Tatiana; Silva, Luciane de Carvalho Sarahyba da; Lopes, Guilherme de Oliveira; Doi, Andre; Hirata, Carlos Eduardo; Yamamoto, Joyce Hisae.
Título: Usefulness of aqueous and vitreous humor analysis in infectious uveitis
Fonte: Clinics;75:e1498, 2020. tab, graf.
Idioma: en.
Resumo: OBJECTIVE: To evaluate the role of intraocular fluid analysis as a diagnostic aid for uveitis. METHODS: Twenty-eight samples (27 patients including 3 HIV-infected patients) with active (n=24) or non-active (n=4) uveitis were submitted to aqueous (AH; n=12) or vitreous humor (VH) analysis (n=16). All samples were analyzed by quantitative PCR for herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV) and Toxoplasma gondii. RESULTS: The positivity of the PCR in AH was 41.7% (5/12), with 50% (2/4) in immunocompetent and 67% (2/3) in HIV+ patients. The positivity of the PCR in VH was 31.2% (5/16), with 13% (1/8) in immunocompetent and 50% (4/8) in immunosuppressed HIV negative patients. The analysis was a determinant in the diagnostic definition in 58% of HA and 50% of VH. CONCLUSION: Even in posterior uveitis, initial AH analysis may be helpful. A careful formulation of possible clinical diagnosis seems to increase the chance of intraocular sample analysis being meaningful.
Descritores: Humor Aquoso/microbiologia
Humor Aquoso/parasitologia
Humor Aquoso/virologia
Uveíte/diagnóstico
Corpo Vítreo/microbiologia
Corpo Vítreo/parasitologia
-Toxoplasma
Uveíte/microbiologia
Uveíte/parasitologia
Uveíte/virologia
Corpo Vítreo/virologia
DNA Viral/análise
Reação em Cadeia da Polimerase
HIV-1
Hospedeiro Imunocomprometido
Simplexvirus/genética
Simplexvirus/imunologia
Herpesvirus Humano 4
Herpesvirus Humano 3/genética
Herpesvirus Humano 3/imunologia
Citomegalovirus/genética
Citomegalovirus/imunologia
Imunocompetência
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


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Monteiro, Talita Antonia Furtado
Vasconcelos, Pedro Fernando da Costa
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Id: biblio-957418
Autor: Vieira, Marcelo Adriano da Cunha e Silva; Castro, Alzira Almeida de Sousa; Henriques, Daniele Freitas; Silva, Eliana Vieira Pinto da; Tavares, Fernando Neto; Martins, Lívia Carício; Guimarães, Lucas Melo; Monteiro, Talita Antonia Furtado; Azevedo, Raimunda do Socorro da Silva; Cruz, Ana Cecíclia Ribeiro; Vasconcelos, Pedro Fernando da Costa.
Título: Encephalitis associated with Zika virus infection and reactivation of the varicella-zoster virus in a Brazilian child
Fonte: Rev. Soc. Bras. Med. Trop;51(3):390-392, Apr.-June 2018. graf.
Idioma: en.
Projeto: CNPq; . CNPq.
Resumo: Abstract We report a case of encephalitis associated with Zika virus infection and reactivation of varicella-zoster virus in the central nervous system of a Brazilian child. This case raises the possibility that reactivation of the latent varicella-zoster virus may be a mechanism of neurological impairment induced by acquired Zika virus infection.
Descritores: Herpesvirus Humano 3/isolamento & purificação
Encefalite por Varicela Zoster/complicações
Zika virus/isolamento & purificação
Infecção por Zika virus/complicações
-Ativação Viral
DNA Viral/líquido cefalorraquidiano
Líquido Cefalorraquidiano/virologia
Encefalite por Varicela Zoster/diagnóstico
Eletroencefalografia
Infecção por Zika virus/diagnóstico
Limites: Humanos
Masculino
Criança
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-1041492
Autor: Fonseca, Ricardo Roberto de Souza; Alves, Ana Cláudia Braga Amoras; Amanajás, Thalita de Almeida; Nogueira, Brenna Magdalena Lima; Menezes, Tatiany Oliveira de Alencar; Siravenha, Leonardo Quintão; Pereira, Danilo Leôncio Aguiar; Machado, Luiz Fernando Almeida; Gilet, Luciana Campêlo da Silva; Menezes, Silvio Augusto Fernandes de.
Título: Absence of cytomegalovirus in gingivitis and chronic periodontitis in HIV-1 patients in Northern Brazil
Fonte: Rev. Soc. Bras. Med. Trop;51(6):809-812, Nov.-Dec. 2018. tab.
Idioma: en.
Projeto: National Council for Scientific and Technological Development.
Resumo: Abstract INTRODUCTION The influence of cytomegalovirus (CMV) on the progression of chronic periodontitis in HIV patients is poorly investigated. METHODS ELISA was used for anti-CMV antibody IgG titer measurements and real-time polymerase chain reaction for qualitative and quantitative CMV detection. Data on the CD4 + T lymphocyte count and plasma HIV viral load were obtained from patient records. RESULTS CMV DNA was detected in samples of subgingival biofilm in only three individuals, two of them with chronic periodontitis (4%) and one with gingivitis (3.3%). CONCLUSIONS The prevalence of CMV is very low both in HIV-1 patients with gingivitis and chronic periodontitis.
Descritores: Infecções por HIV/complicações
Carga Viral
Citomegalovirus/isolamento & purificação
Periodontite Crônica/virologia
Gengivite/virologia
-DNA Viral
HIV-1
Infecções por Citomegalovirus/virologia
Contagem de Linfócito CD4
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Masculino
Feminino
Adolescente
Responsável: BR1.1 - BIREME


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Id: biblio-1003137
Autor: Salles, Nanci Alves; Nishiya, Anna Shoko; Ferreira, Suzete Cleusa; Rocha, Vanderson Geraldo; Mendrone-Junior, Alfredo.
Título: Detection of HIV-1 infections in blood donors during the pre-seroconversion window period in São Paulo, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;52:e20180432, 2019. tab.
Idioma: en.
Resumo: Abstract By decreasing the pre-seroconversion window period, nucleic acid testing (NAT) has improved the safety of blood products and reduced the risk of transfusion-transmitted infections. Between 2011 and 2017, NAT determinations for approximately 898,202 donations were performed at Fundação Pró-Sangue/Hemocentro de São Paulo (FPS-HSP). Three seronegative HIV-viremic donations were detected. The NAT yield rate per million donations was 3.34 for HIV, and the acute HIV-1 infections detected are described, followed by a brief review of the situation in Brazil.
Descritores: Doadores de Sangue
DNA Viral/sangue
RNA Viral/sangue
Infecções por HIV/diagnóstico
HIV-1/genética
Técnicas de Amplificação de Ácido Nucleico
Limites: Humanos
Masculino
Adulto
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


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Id: biblio-1020443
Autor: Figueiredo, Regina Maria Pinto de; Souza, Victor Costa de; Nascimento, Valdinete Alves do; Naveca, Felipe Gomes.
Título: Human parvovirus B19 genotype 1 in suspected dengue patients of Tefé, Amazonas State, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;52:e20190304, 2019. tab, graf.
Idioma: en.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Fundação de Amparo à Pesquisa do Estado do Amazonas; . Conselho Nacional de Desenvolvimento Científico e Tecnológico; . Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Resumo: Abstract INTRODUCTION: Human parvovirus B19 (B19V) is a common pathogen, which on infection causes variety of clinical conditions from benign self-limiting exanthematous disease and other similar pathologies to fetal death. METHODS: We collected 341 serum samples between the first and fourth day after the onset of symptoms from all patients suspected of dengue fever who were attended at Regional Hospital of Tefé. Initially, patients were screened for malaria by blood smear test and negative samples were sent to Fundação de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD) situated in Manaus (AM) for dengue testing using semi-nested multiplex PCR. Further, we investigated 44 malaria and dengue-negative samples of children for B19V DNA by nested-PCR. Positive samples were analyzed by BLAST against entire public non-redundant nucleotide database and genotyped by phylogenetic analyses using neighbor-joining clustering method. RESULTS: Eight samples (18.2%) were found to be PCR positive. Fever, headache, ocular pain, and/or muscle pain were reported as the most frequent symptoms by the patients and none were diagnosed with rash at the time of sample collection. Phylogenetic analysis of major capsid protein 2 (VP2) and VP3 coding region showed high similarity with B19V genotype 1. CONCLUSIONS: Our results reveal the spread of B19V genotype 1 in Tefé. Moreover, our results emphasize the significance of laboratorial differential diagnosis using molecular techniques in patients with acute febrile, and thereby aid the health surveillance system in improving patient care even in the remote areas of Amazon.
Descritores: DNA Viral/sangue
Parvovirus B19 Humano/genética
Infecções por Parvoviridae/diagnóstico
Infecções por Parvoviridae/virologia
Dengue/diagnóstico
-Filogenia
Brasil
Reação em Cadeia da Polimerase
Genótipo
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Idoso
Adulto Jovem
Responsável: BR1.1 - BIREME



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