Base de dados : LILACS
Pesquisa : D13.444.600.723.480 [Categoria DeCS]
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Texto completo SciELO Chile
Texto completo
Id: lil-430695
Autor: Fcollins, James.
Título: Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
Fonte: Biol. Res;39(1):25-37, 2006. tab.
Idioma: en.
Projeto: NIH; . HRSA.
Resumo: Previous studies revealed novel genetic changes in the duodenal mucosa of iron-deprived rats during post-natal development. These observations are now extended to compare the genetic response to iron deficiency in the duodenum versus jejunum of 12-wk-old rats. cRNA samples were prepared from the duodenal and jejunal mucosa of three groups each of control and iron-deficient rats and hybridized with RAE 230A and 230B gene chips (Affymetrix). Stringent data reduction strategies were employed. Results showed that several genes were similarly induced in both gut segments, including DMT1, Dcytb, transferrin receptor 1, heme oxygenase 1, metallothionein, the Menkes copper ATPase (ATP7A), tripartitie motif protein 27, and the sodium-dependent vitamin C transporter. However, a subset of genes showed regulation in only one or the other gut segment. In duodenum only, gastrokine 1, trefoil factor 1 and claudin 2 were induced by iron-deficiency. Other genes previously identified were only regulated in the duodenum. Overall, these studies demonstrate similarities and distinct differences in the genetic response to iron deprivation in the duodenum versus jejunum and provide evidence that more distal gut segments also may play a role in increasing iron absorption in iron-deficiency anemia.
Descritores: Duodeno/metabolismo
Absorção Intestinal/genética
Mucosa Intestinal/metabolismo
Análise de Sequência com Séries de Oligonucleotídeos/métodos
Ratos Sprague-Dawley
RNA Complementar/análise
Limites: Animais
Responsável: BR1.1 - BIREME

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Texto completo SciELO Brasil
Texto completo
Id: lil-425790
Autor: Zander, T; Yunes, J. A; Cardoso, A. A; Nadler, L. M.
Título: Rapid, reliable and inexpensive quality assessment of biotinylated cRNA
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;39(5):589-593, May 2006. ilus, tab.
Idioma: en.
Projeto: Frauke Weiskam-Christel Ruranski Foundation; . NIH; . Leukemia and Lymphoma Society; . NIH.
Resumo: The interpretation of oligonucleotide array experiments depends on the quality of the target cRNA used. cRNA target quality is assessed by quantitative analysis of the representation of 5' and 3' sequences of control genes using commercially available Test arrays. The Test array provides an economically priced means of determining the quality of labeled target prior to analysis on whole genome expression arrays. This manuscript validates the use of a duplex RT-PCR assay as a faster (6 h) and less expensive (6 were chosen and classified as degraded cRNAs, and 31 samples with a ß-actin 3'/5' ratio <6 were selected as good quality cRNAs. Blinded samples were then used for the RT-PCR assay. After gel electrophoresis, optical densities of the amplified 3' and 5' fragments of ß-actin were measured and the 3'/5' ratio was calculated. There was a strong correlation (r² = 0.6802) between the array and the RT-PCR ß-actin 3'/5' ratios. Moreover, the RT-PCR 3'/5' ratio was significantly different (P < 0.0001) between undegraded (mean ± SD, 0.34 ± 0.09) and degraded (1.71 ± 0.83) samples. None of the other parameters analyzed, such as i) the starting amount of RNA, ii) RNA quality assessed using the Bioanalyzer Chip technology, or iii) the concentration and OD260/OD280 ratio of the purified biotinylated cRNA, correlated with cRNA quality.
Descritores: Análise de Sequência com Séries de Oligonucleotídeos/métodos
RNA Complementar/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Limites: Humanos
Tipo de Publ: Estudo de Validação
Responsável: BR1.1 - BIREME

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Id: lil-211512
Autor: Ayerra de Holstein, Bethy.
Título: Interferón, sin pausa / Interferon, without a pause
Fonte: Rev. Asoc. Méd. Argent;97(1):3-6, ene.-feb. 1984.
Idioma: es.
Descritores: Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem
Indutores de Interferon/síntese química
Interferon-alfa/uso terapêutico
Interferon beta/uso terapêutico
Interferon gama/uso terapêutico
Metástase Neoplásica
Neoplasias/tratamento farmacológico
RNA Complementar
Interferência Viral
-Encefalite Viral/tratamento farmacológico
Hepatite Crônica/terapia
Herpes Genital/tratamento farmacológico
Herpes Labial/tratamento farmacológico
Limites: Humanos
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas

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Id: lil-180079
Autor: Lippman, M.
Título: Terapia biológica del cáncer de mama / Biological therapy of the breast cancer
Fonte: Rev. argent. mastología;14(45):228-34, sept. 1995. tab.
Idioma: es.
Conferência: Apresentado em: Congreso Argentino de Mastología, 5 y Congreso Ibero-Americano de Mastología, 2, Buenos Aires, 17-20 sept. 1995.
Descritores: Tratamento Biológico
Neoplasias da Mama/tratamento farmacológico
Neoplasias da Mama/imunologia
Neoplasias da Mama/terapia
Técnicas In Vitro
Metástase Neoplásica
-Estrogênios/uso terapêutico
Exotoxinas/uso terapêutico
Fator de Crescimento Epidérmico
Genes erbB-2
RNA Complementar/isolamento & purificação
Tamoxifeno/uso terapêutico
Limites: Humanos
Responsável: AR1.1 - Biblioteca Rafael Herrera Vegas

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