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Id: biblio-1254957
Autor: Iqbal, Amjad; Yang, Yaodong; Wu, Yi; Li, Jing; Hamayun, Muhammad; Hussain, Anwar; Shah, Farooq.
Título: An easy and robust method for the isolation of high quality RNA from coconut tissues
Fonte: Electron. j. biotechnol;48:78-85, nov. 2020. ilus, tab.
Idioma: en.
Projeto: International Science and Technology Cooperation projects of Hainan Province; . Fundamental Scientific Research Funds for Chinese Academy of Tropical Agricultural Sciences.
Resumo: BACKGROUND: Coconut tissues consist of a complex network of polysaccharides, proteins, polyphenols, and lipids that can bind to nucleic acids and pose difficulty in isolation. Certainly, a vigorous method is required to isolate high quality and quantity of RNA from such tissues for the purpose of downstream experiments. In this paper, we discuss a newly developed method for the Isolation of RNA from Complex Matrices (IRCM) method from coconut tissues. RESULTS: The method is robust, cheap, and efficient for the extraction of quality RNA in high quantities from the solid endosperm of stored and fresh coconut (150 µg/g FW with A260/280 = 1.89 and 247.5 µg/g FW with A260/280 = 1.91), coconut apple (263.8 µg/g FW with A260/280 = 1.97), and coconut bud (1052.5 µg/g FW with A260/280 = 2.00). The other well established methods, such as Method of RNA Isolation from Palm (MRIP), Cetyl Trimethyl Ammonium Bromide (CTAB), TRIZOL, and RNA plant kit failed to isolate quality RNA in appreciable quantities from the coconut tissues. Furthermore, the resultant RNA performed well in the downstream experiment, that is, RT-PCR for the production and amplification of cDNA. CONCLUSIONS: From the study, we concluded that the present method will play a vital role in the extraction of high quality RNA from complex matrices in a short time.
Descritores: RNA/isolamento & purificação
Cocos/genética
-Reação em Cadeia da Polimerase Via Transcriptase Reversa
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1147090
Autor: Michelon, Cleonice Maria; Piccinini, Alexandre.
Título: Presença de RNA do SARS-CoV-2 em fezes de pacientes com COVID-19 / Presence of SARS-CoV-2 RNA in faeces of COVID-19 patients
Fonte: Rev. bras. anal. clin;52(2):168-172, 20200630. ilus.
Idioma: pt.
Resumo: A COVID-19, doença causada pelo novo Coronavírus, alastrou-se rapidamente por todos os continentes promovendo uma pandemia. Estudos relacionados à fisiopatologia da COVID-19 demonstraram que o vírus SARS-CoV-2 invade células da mucosa intestinal, sendo eliminado nas fezes, alertando para possibilidade da transmissão da doença por via fecal-oral. A presença do vírus nas fezes aventou também a expectativa de utilizar essa amostra biológica para fins diagnósticos. Nesta revisão, resumimos os estudos recentes relacionados à investigação da presença do RNA do SARS-CoV-2 nas fezes de pacientes com COVID-19.
Descritores: RNA
Infecções por Coronavirus
Fezes
Betacoronavirus
Tipo de Publ: Revisão Sistemática
Responsável: BR408.1 - Biblioteca da Faculdade de Medicina - BFM


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Id: biblio-1247370
Autor: Brasil. Ministério da Saúde. Coordenação de Monitoramento e Avaliação de Tecnologias em Saúde.
Título: Rendesivir para tratamento de pacientes com COVID-19 / Rendesivir for treatment of patients with Covid-19.
Fonte: s.l; s.n; mar. 2021.
Idioma: pt.
Descritores: Antivirais/uso terapêutico
Pneumonia Viral/tratamento farmacológico
RNA/farmacologia
Infecções por Coronavirus/tratamento farmacológico
Betacoronavirus/efeitos dos fármacos
-Avaliação da Tecnologia Biomédica
Análise Custo-Benefício
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
GOVERNMENT PUBLICATIONS
Responsável: BR1.1 - BIREME


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Id: biblio-1005720
Autor: Bezerra, Daniela da Silva; Neves, Beatriz Gonçalves; Guedes, Sarah Florindo de Figueiredo; Regis, Wanessa Fernandes Matias; Stipp, Rafael Nóbrega; Rodrigues, Lidiany Karla Azevedo.
Título: Extraction and purification of RNA from human carious dentine: an approach to enable bacterial gene expression studies / Extração e purificação de RNA proveniente de dentina humana cariada: uma abordagem para viabilizar estudos de expressão gênica
Fonte: J. Health Biol. Sci. (Online);7(2):145-151, abr.-jun. 2019.
Idioma: en.
Resumo: Background: RNA isolation from bacteria within dentine caries lesions could be difficult due to reduced amount of collectable biomass and high mRNA instability. Attempting to overcome this challenge we describe one protocol developed to extract and purify total RNA from dentine lesions. Objective: customize a bacterial RNA extraction and purification method from human carious dentine. Methods: quantity and purity of extracted RNA were measured with a microvolume UV-VIS spectrophotometer, RNA integrity was assessed by standard denaturing agarose gel electrophoresis and images were captured under ultraviolet light with camera and analyzed. DNase treatment removed genomic DNA and an additional step of purification was carried out in silica spin column. Results: final yield (ng/µl) was 67.01 ± 22.33, absorbance ratio A260/A280 = 2.0 ± 0.07 and RNA integrity were obtained. The purified samples were reversely transcribed and the expression of atpD and fabM gene from Streptococcus mutans analyzed by quantitative real-time PCR. Conclusion: the extraction methodology developed produced high-quality RNA from dentine microbiota for transcriptional analysis.

Introdução: o isolamento de RNA de bactérias dentro de lesões de dentina cariada pode ser difícil devido à quantidade reduzida de biomassa e alta instabilidade de mRNA. Na tentativa de superar esse desafio, descrevemos um protocolo desenvolvido para extrair e purificar o RNA total das lesões dentinárias. Objetivo: personalizar um método de extração e purificação de RNA bacteriano a partir da dentina cariada humana. Métodos: a quantidade e a pureza do RNA extraído foram medidas com um espectrofotômetro UV-VIS de microvolume, a integridade do RNA foi avaliada por eletroforese em gel de agarose desnaturante padrão e as imagens foram capturadas sob luz ultravioleta e analisadas. O tratamento com DNase removeu o DNA genômico e uma etapa adicional de purificação foi realizada em coluna de spin de sílica. Resultados: o rendimento final (ng / µl) foi de 67,01 ± 22,33, a razão de absorbância A260 / A280 = 2,0 ± 0,07 e a integridade do RNA foram obtidas. As amostras purificadas foram transcritas reversamente e a expressão do gene atpD e fabM de Streptococcus mutans analisadas por PCR quantitativo em tempo real (RT-qPCR). Conclusão: a metodologia de extração desenvolvida produziu RNA de alta qualidade da microbiota dentinária para análises transcricionais.
Descritores: RNA
Dentina
-Streptococcus mutans
Expressão Gênica
Responsável: BR1780.2


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Id: biblio-1177997
Autor: Pastrian Soto, Gabriel.
Título: Potenciales agentes terapéuticos contra el COVID-19 basados en el bloqueo e inhibición del ciclo de vida viral y en el síndrome de tormenta de citoquinas / Potential therapeutic agents against COVID-19 based on blocking and inhibition of the viral life cycle and the cytokine storm syndrome
Fonte: An. Fac. Cienc. Méd. (Asunción);53(3):131-146, 20201201.
Idioma: en.
Resumo: La pandemia de COVID-19, causada por SARS-CoV-2, es considerara la mayor emergencia sanitaria en un siglo. Clínicamente, la mayoría de los pacientes tienen síntomas leves a moderados. Sin embargo, pacientes de edad avanzada o con comorbilidades pueden desarrollar una de las complicaciones más severas de COVID-19, es decir, el síndrome de tormenta de citoquinas. Actualmente, no existen tratamientos aprobados para SARS-CoV-2. Mientras tanto, las estrategias terapéuticas se basan en la experiencia previa con otros virus. En este artículo se revisarán los diferentes agentes terapéuticos propuestos para el tratamiento de COVID-19 basados en el bloqueo e inhibición del ciclo de vida viral de SARS-CoV-2, y para el tratamiento del síndrome de tormenta de citoquinas. Se realizó una revisión narrativa mediante búsqueda en la base de datos PubMed. Entre los principales objetivos terapéuticos contra el SARS-CoV-2 están la proteína estructural principal Spike y las enzimas virales proteasa similar a la 3-quimotripsina, la proteasa viral similar a la papaína y la ARN-polimerasa dependiente de ARN. Remdesivir, un antiviral análogo a la adenosina que inhibe a la ARN-polimerasa dependiente de ARN, es considerado el fármaco más prometedor en el tratamiento de COVID-19. No obstante, su eficacia aún no se ha determinado. En el síndrome de tormenta de citoquinas, la lesión tisular causada por el virus puede inducir la producción exagerada de citoquinas proinflamatorias como la interleucina-6. Tocilizumab, un anticuerpo monoclonal que bloquea receptores de interleucina-6 y corticosteroides como la metilprednisolona pueden ser opciones terapéuticas en el tratamiento de la severidad del síndrome.

The COVID-19 pandemic, caused by SARS-CoV-2, is considered as the major health emergency in a century. Clinically, most patients have mild to moderate symptoms. Nevertheless, elderly or with comorbidities patients may develop one of the most severe complication of COVID-19, that is, the cytokine storm syndrome. Currently, there are no approved treatments for SARS-CoV-2. Meanwhile, therapeutic strategies are based on previous experience with other viruses. This article will review the different therapeutic agents proposed for the treatment of COVID-19 based on the blocking and inhibition of the viral life cycle of SARS-CoV-2, and for the treatment of cytokine storm syndrome. A narrative review was performed by searching in the PubMed database. Among the main therapeutic target against SARS-CoV-2 are the major structural protein Spike and viral enzymes 3-chymotrypsin-like protease, viral papain-like protease, and RNA-dependent RNA polymerase. Remdesivir, an adenosine analogue antiviral that inhibits RNA-dependent RNA polymerase, is considered the most promising drug in the treatment of COVID-19. Nonetheless, its efficacy has not yet been determined. In the cytokine storm syndrome, the tissue injury caused by the virus may induce the exaggerated production of pro-inflammatory cytokines such as interleukin-6. Tocilizumab, a monoclonal antibody that blocks interleukin-6 receptors, and corticosteroids such as methylprednisolone may be therapeutic options in treating the severity of the syndrome.
Descritores: RNA Polimerase Dependente de RNA
RNA
Metilprednisolona
Adenosina
Citocinas
Interleucina-6
Corticosteroides
Infecções por Coronavirus
Betacoronavirus
-Pandemias
Objetivos
Estágios do Ciclo de Vida
Responsável: PY1.4 - Biblioteca


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Texto completo SciELO Chile
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Id: biblio-983944
Autor: Ren, Yongzhe; Yue, Huifang; Li, Le; Xu, Yanhua; Wang, Zhiqiang; Xin, Zeyu; Lin, Tongbao.
Título: Identification and characterization of circRNAs involved in the regulation of low nitrogen-promoted root growth in hexaploid wheat
Fonte: Biol. Res;51:43, 2018. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . National Key Research and Development Program of China; . National Key Research and Development Program of China; . Natural Science Foundation of Henan province; . State Key Laboratory Program.
Resumo: BACKGROUND: CircRNAs are widespread in plants and play important roles in response to abiotic stresses. Low nitrogen (LN) promotes the growth of plant root system, allowing it to explore more nitrogen. However, whether circRNAs involved in the response to LN stress and the regulation of LN-promoted root growth in wheat remains unclear. METHODS: Two wheat varieties (LH9 and XN979) with contrasting root phenotypes to LN stress were used as materials to identify circRNAs under control and LN conditions by using high-throughput sequencing technology. RESULTS: Six differentially expressed circRNAs (DECs) involved in the common response to LN stress and 23 DECs involved in the regulation of LN-promoted root growth were successfully identified. GO analysis of the DEC-host genes involved in the regulation of LN-promoted root growth showed that GO terms related to biological regulation, responses to stimuli and signalling were significantly enriched. Moreover, seven DECs were predicted to have miRNA binding sites and may serve as miRNA sponges to capture miRNAs from their target genes. CONCLUSIONS: LN stress altered the expression profiles of circRNAs in wheat. This is the first report of LN stress responsive circRNAs in plants. Our results provided new clues for investigating the functions of circRNAs in response to LN stress and in the regulation of LN-promoted wheat root growth.
Descritores: Estresse Fisiológico/fisiologia
Triticum/crescimento & desenvolvimento
RNA/isolamento & purificação
Raízes de Plantas/crescimento & desenvolvimento
Regulação da Expressão Gênica de Plantas/fisiologia
Nitrogênio/metabolismo
-Triticum/fisiologia
RNA/metabolismo
RNA Circular
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Chile
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Id: biblio-1011421
Autor: Xu, Yanhua; Ren, Yongzhe; Lin, Tongbao; Cui, Dangqun.
Título: Identification and characterization of CircRNAs involved in the regulation of wheat root length
Fonte: Biol. Res;52:19, 2019. tab, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Natural Science Foundation of Henan province; . State Key Laboratory Program.
Resumo: BACKGROUND: Recent studies indicate that circular RNAs (circRNAs) may play important roles in the regulation of plant growth and development. Plant roots are the main organs of nutrient and water uptake. However, whether circRNAs involved in the regulation of plant root growth remains to be elucidated. METHODS: LH9, XN979 and YN29 are three Chinese wheat varieties with contrasting root lengths. Here, the root circRNA expression profiles of LH9, XN979 and YN29 were examined by using high-throughput sequencing technology. RESULTS: Thirty-three and twenty-two differentially expressed circRNAs (DECs) were identified in the YN29-LH9 comparison and YN29-XN979 comparison, respectively. Among them, ten DECs coexisted in both comparisons. As the roots of both LH9 and XN979 were significantly larger and deeper than YN29, the ten DECs coexisting in the two comparisons were highly likely to be involved in the regulation of wheat root length. Moreover, three of the ten DECs have potential miRNA binding sites. Real-time PCR analysis showed that the expression levels of the potential binding miRNAs exhibited significant differences between the long root plants and the short root plants. CONCLUSIONS: The expression levels of some circRNAs exhibited significant differences in wheat varieties with contrasting root phenotypes. Ten DECs involved in the regulation of wheat root length were successfully identified in which three of them have potential miRNAs binding sites. The expression levels of putative circRNA-binding miRNAs were correlated with their corresponding circRNAs. Our results provide new clues for studying the potential roles of circRNAs in the regulation of wheat root length.
Descritores: Triticum/crescimento & desenvolvimento
RNA/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
-Triticum/fisiologia
Regulação para Baixo/fisiologia
Regulação para Cima/fisiologia
Regulação da Expressão Gênica de Plantas
Sequenciamento de Nucleotídeos em Larga Escala
Reação em Cadeia da Polimerase em Tempo Real
RNA Circular
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Cuba
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Id: biblio-961279
Autor: Corrales Alonso, Sahilí; Morales Díaz, Mariuska; Ávalos García, Roxana; González Lecusay, Amaury; Díaz Ramos, Danais; Celestrin Tápanes, Homero.
Título: Comportamiento virológico de pacientes con hepatitis C tratados con antivirales cubanos / Virological behavior of patients with hepatitis C treated with Cuban antivirals
Fonte: Rev. medica electron;40(4):1045-1058, jul.-ago. 2018. ilus.
Idioma: es.
Resumo: Introducción: el seguimiento virológico de los pacientes con hepatitis C se realiza mediante la determinación cuantitativa del ácido ribonucleico viral por técnicas de biología molecular. Objetivos: evaluar el comportamiento virológico de los pacientes con hepatitis crónica C tratados con antivirales cubanos. Materiales y métodos: se realizó un estudio descriptivo, prospectivo en 45 pacientes con hepatitis crónica C atendidos en Consulta de Hepatología del Hospital Universitario Faustino Pérez, de Matanzas, en el período comprendido entre enero 2014 a diciembre 2017, tratados durante 48 semanas con PEG-heberon y ribavirina. De ellos se analizaron las características basales así como los diferentes tipos de respuesta al tratamiento según resultados virológicos. Resultados: predominaron los pacientes del sexo femenino, menores de 45 años, vírgenes de tratamiento y con cargas virales basales altas. Se alcanzó la respuesta virológica rápida en el 31,1%, la temprana total en el 19,4 %, al final del tratamiento en el 77,1% y la respuesta virológica sostenida en el 59,3%. Entre los respondedores predominaron los rápidos con respuesta virológica sostenida y entre los no respondedores, los nulos. Conclusiones: los estudios cuantitativos de ácido ribonucleico viral son esenciales para el seguimiento de los pacientes con hepatitis C ya que a través de sus determinaciones basales, durante el tratamiento y posterior a este, puede evaluarse la respuesta al tratamiento (AU).

Introduction: the virological follow-up of patients with hepatitis C is made through the quantitative determination of the viral ribonucleic acid using techniques of molecular biology. Objectives: to assess the virological behavior of patients with hepatitis C treated with Cuban antivirals. Materials and methods: a prospective, descriptive study was carried out in 45 patients with hepatitis C who attended the Consultation of Hepatology of the University Hospital "Faustino Pérez", of Matanzas, in the period from January 2014 to December 2017, treated with PEG-eberon and ribavirin for 48 weeks. Their basal characteristics were analyzed and also the different kinds of answer to the treatment according to the virological results. Results: female sex, patients aged less than 45 years old, non-treated before and with high viral loads. The fast virological answer was reached in 31 % of the patients, and the total early answer in 19.4 %; at the end of the treatment in 77.1 %, and the sustained virological answer in 59.3 % of the patients. Among the answering ones predominated the fast with sustained virological answer, and among the non-answering predominated the null ones. Conclusions: quantitative studies of viral ribonucleic acid are essential for the follow-up of patients with hepatitis C, because through their basal determinations, during and after the treatment, the answer to the treatment can be evaluated (AU).
Descritores: Antivirais/uso terapêutico
Hepatite C/virologia
-Pacientes
RNA/efeitos dos fármacos
RNA/uso terapêutico
RNA/farmacologia
Resultado do Tratamento
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo Observacional
Responsável: CU424.1 - Centro Provincial de Información de Ciencias Médicas


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Texto completo SciELO Cuba
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Id: biblio-1139050
Autor: Hart Casares, Marcia.
Título: Diagnóstico microbiológico de SARS-COV 2 / Microbiological diagnosis of SARS-VOC 2
Fonte: Rev. cuba. med;59(2):e1344, abr.-jun. 2020.
Idioma: es.
Resumo: Introducción: La enfermedad producida por el nuevo coronavirus constituye un reto para los sistemas de salud. En estos tiempos de pandemia disponer de pruebas que ayuden a un diagnóstico temprano e incluso a detectar pacientes asintomáticos es una de las claves para disminuir los contagios y evitar la propagación. Objetivo: Revisar los aspectos más importantes en el diagnóstico del nuevo coronavirus. Desarrollo: La detección de ARN de SARS-CoV2 en muestras respiratorias, es la técnica de referencia y de elección para el diagnóstico microbiológico de COVID-19. Tomando la muestra de la parte posterior de la faringe y de las fosas nasales puede detectarse la presencia del virus. La detección de antígenos es un tipo de prueba de diagnóstico rápido la cual detecta la presencia de proteínas virales (antígenos) expresadas por el virus de la COVID-19. La detección de los anticuerpos generados en el organismo huésped infectado es una de las técnicas más utilizadas a nivel mundial en grandes poblaciones, incluso como pesquizaje, aunque su interpretación puede requerir intervención de médicos especializados. También está basada en la detección de anticuerpos del tipo IgM e IgG y algunas presentan la detección de anticuerpos IgA. Conclusiones: La interpretación de las pruebas serológicas debe realizarse con cautela, teniendo en cuenta sus limitaciones, y evaluarlas acorde a la situación clínica del paciente y de los resultados de la prueba de referencia(AU)

Introduction: The disease caused by the new coronavirus constitutes a challenge for health systems. In these times of pandemic, having tests that help early diagnosis and even detect asymptomatic patients is one of the keys to reducing infections and preventing the spread. Objective: To review the most important aspects in the diagnosis of the new coronavirus. Findings: Detection of SARS-CoV2 RNA in respiratory samples is the reference and technique of choice for the microbiological diagnosis of COVID-19. By taking samples from the back of the pharynx and the nostrils, the presence of the virus can be detected. Antigen detection is a type of rapid diagnostic test which detects the presence of viral proteins (antigens) expressed by COVID-19 virus. Detection of the antibodies generated in the infected host organism is one of the most widely used techniques worldwide in large populations, even as screening, although its interpretation may require the intervention of specialists. It is also based on the detection of IgM and IgG type antibodies and some have the detection of IgA antibodies. Conclusions: The interpretation of serological tests should be done with caution, taking into account the limitations, and assessing them according to the patient's clinical situation and the results of the reference test(AU)
Descritores: RNA/uso terapêutico
Infecções por Coronavirus/microbiologia
Diagnóstico Precoce
-Imunoglobulina M/análise
Testes Sorológicos/métodos
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Revisão
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: biblio-1117111
Autor: Atkinson, Emily G; Marcial, Alejandro; Sánchez, Zuleima; Porter, Christian; Plotkin, Lilian I.
Título: MLO-Y4 osteocytic cell clones express distinct gene expression patterns characteristic of different stages of osteocyte differentiation / Clones de las células osteocíticas MLO-Y4 tienen diferentes patrones de expresión génica característicos de diferentes estadíos de diferenciación osteocítica
Fonte: Actual. osteol;13(3):207-213, Sept - DIc. 2017. ilus, graf.
Idioma: en.
Resumo: Osteocytes are the most abundant bone cell and are formed when osteoblasts become embedded in the bone matrix. Through changes in gene expression and paracrine effects, osteocytes regulate the number of osteoblasts, bone forming cells, and osteoclasts, bone resorbing cells, which are needed to maintain bone mass. MLO-Y4 is the better characterized osteocytic cell line; however, lacks expression of sclerostin, the product of the SOST gene, which is fundamental for osteocyte function and blocks bone formation. With the objective to isolate MLO-Y4 clones with different gene expression profiles, we performed cultures at very low density of MLO-Y4 cells stably transfected with nuclear green fluorescent protein (MLOnGFP). Cell morphology was visualized under a fluorescence microscope. Once the cells reached 80% confluency, RNA was extracted and quantitative real time PCR was performed. Clones exhibit different sizes and morphology, with some cells showing a spindle-like shape and others with abundant projections and a star-like shape. Gene expression also differed among clones. However, none of the clones examined expressed SOST. We conclude that the MLO-nGFP clones constitute a useful tool to study osteocyte differentiation and the role of osteocytes in the control of bone formation and resorption in vitro. (AU)

Los osteocitos son las células más abundantes del hueso y se forman cuando los osteoblastos se encuentran rodeados de matriz ósea. A través de cambios en la expresión génica y efectos paracrinos, los osteocitos controlan el número de osteoblastos que forman el hueso, y osteoclastos que resorben el hueso, células necesarias para mantener la masa ósea. Las células MLO-Y4 son la línea celular osteocítica más investigada; sin embargo, no expresan esclerostina, el pro esclerostina, el producto del gen SOST que bloquea la formación ósea y es indispensable para la función de los osteocitos. Con el objetivo de aislar clones de las células MLO-Y4 con diferentes perfiles de expresión génica, realizamos cultivos a muy baja densidad de las células transfectadas en forma estable con proteína verde fluorescente nuclear (MLO-nGFP). La morfología celular fue evaluada utilizando un microscopio de fluorescencia. Una vez que las células alcanzaron el 80% de confluencia, el ARN fue extraído y analizado por PCR cuantitativa en tiempo real. Las células de los diferentes clones tienen diferentes tamaños y morfología, algunas células son fusiformes y otras con proyecciones citoplasmáticas abundantes y en forma de estrella. La expresión de los genes también varió en los distintos clones. Sin embargo, ninguno de ellos expresó SOST. En conclusión, los clones de las células MLO-nGFP constituyen una herramienta útil para estudiar la diferenciación de los osteocitos y el rol de estas células en el control de la formación y resorción ósea in vitro. (AU)
Descritores: Osteoblastos/citologia
Osteoclastos/citologia
Osteócitos/citologia
Linhagem Celular
Células Clonais/citologia
-Osteoblastos/metabolismo
Osteoclastos/metabolismo
Osteócitos/metabolismo
Osteogênese/genética
Reabsorção Óssea/genética
Técnicas In Vitro
RNA/análise
Expressão Gênica
Reação em Cadeia da Polimerase
Colágeno/genética
Fosfatase Alcalina/metabolismo
Fluorescência
Antibacterianos/administração & dosagem
Limites: Humanos
Masculino
Feminino
Tipo de Publ: Estudo Clínico
Responsável: AR2.1 - Biblioteca Central



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