Base de dados : LILACS
Pesquisa : D13.444.735.490 [Categoria DeCS]
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Texto completo SciELO Saúde Pública
Szwarcwald, Célia Landmann
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Id: lil-776715
Autor: Malta, Deborah Carvalho; Szwarcwald, Celia Landmann.
Título: Pesquisa Nacional de Saúde e a Saúde Pública Brasileira / National Health Survey and Public Health in Brazil
Fonte: Rev. bras. epidemiol;18(supl.2):1-2, Out.-Dez. 2015.
Idioma: en.
Descritores: Begomovirus/genética
Lycopersicon esculentum/virologia
Doenças das Plantas/virologia
Plantas Geneticamente Modificadas/virologia
Interferência de RNA
Vírus Satélites/genética
-Begomovirus/fisiologia
Lycopersicon esculentum/genética
Lycopersicon esculentum/imunologia
Omã
Doenças das Plantas/genética
Doenças das Plantas/imunologia
Doenças das Plantas/prevenção & controle
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/imunologia
RNA de Cadeia Dupla/genética
RNA de Cadeia Dupla/metabolismo
RNA Viral/genética
RNA Viral/metabolismo
Vírus Satélites/fisiologia
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 10 LILACS  
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Texto completo SciELO Brasil
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Id: lil-763064
Autor: Wang, Chenghe; Chen, Zhong; Wu, Jia; Zhang, Yan; Hu, Jia; Ge, Qiangqiang; Wang, Tao; Yang, Weimin; Xu, Hua; Liu, Jihong; Ye, Zhangqun.
Título: Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD
Fonte: Int. braz. j. urol;41(4):764-772, July-Aug. 2015. graf.
Idioma: en.
Projeto: National Natural Science Foundation of PR China.
Resumo: ABSTRACTPurpose:RNA activation (RNAa) is a mechanism of gene activation triggered by promoter-targeted small double stranded RNAs (dsRNAs), also known as small activating RNAs (saRNAs). Myogenic regulatory factor MyoD is regarded as the master activator of myogenic differentiation cascade by binding to enhancer of muscle specific genes. Stress urinary incontinence (SUI) is a condition primarily resulted from urethral sphincter deficiency. It is thus expected that by promoting differentiation of adipose-derived stem cells (ADSCs) into myoblasts by activating MyoD gene through RNAa may offer benefits to SUI.Materials and Methods:Rats ADSCs were isolated, proliferated in vitro, and identified by flow cytometry. Purified ADSCs were then transfected with a MyoD saRNA or control transfected. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to detect MyoD mRNA and protein expression, respectively. Immunocytochemical staining was applied to determine the expression of desmin protein in transfected cells. Cell viability was measured by using CellTiter 96® AQueous One Solution Cell Proliferation Assay kit.Results:Transfection of a MyoD saRNA (dsMyoD) into ADSCs significantly induced the expression of MyoD at both the mRNA and protein levels, and inhibited cell proliferation. Desmin protein expression was detected in dsMyoD treated ADSCs 2 weeks later.Conclusion:Our findings show that RNAa mediated overexpression of MyoD can promote transdifferentiation of ADSCs into myoblasts and may help treat stress urinary incontinence (SUI)–a condition primarily resulted from urethral sphincter deficiency.
Descritores: Tecido Adiposo/citologia
Diferenciação Celular/genética
Desmina/metabolismo
Proteína MyoD/genética
Mioblastos/citologia
RNA de Cadeia Dupla
Células-Tronco/citologia
-Western Blotting
Sobrevivência Celular
Citometria de Fluxo
Expressão Gênica
Imuno-Histoquímica
Proteína MyoD/metabolismo
Mioblastos/metabolismo
Cultura Primária de Células
Regiões Promotoras Genéticas/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Células-Tronco/metabolismo
Transfecção
Ativação Transcricional/fisiologia
Uretra/patologia
Incontinência Urinária por Estresse/genética
Incontinência Urinária por Estresse/metabolismo
Limites: Animais
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  3 / 10 LILACS  
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Id: lil-746453
Autor: Assmann, Taís Silveira; Brondani, Letícia de Almeida; Bouças, Ana Paula; Canani, Luís Henrique; Crispim, Daisy.
Título: Toll-like receptor 3 (TLR3) and the development of type 1 diabetes mellitus
Fonte: Arch. endocrinol. metab. (Online);59(1):4-12, 02/2015. tab, graf.
Idioma: en.
Resumo: Type 1 diabetes mellitus (T1DM) is a chronic, progressive autoimmune disease characterized by metabolic decompensation often leading to dehydration and ketoacidosis. Viral agents seem to play an important role in triggering the autoimmune destruction that leads to the development of T1DM. Among several viral strains investigated so far, the enterovirus family has been consistently associated with the onset of T1DM in humans. One of the mediators of viral damage is the double-stranded RNA (dsRNA) generated during replication and transcription of viral RNA and DNA. The Toll-like receptor 3 (TLR3) gene codes for an endoplasmic receptor of the pattern-recognition receptors (PRRs) family that recognizes dsRNA, plays an important role in the innate immune response triggered by viral infection. Binding of dsRNA to the TLR3 triggers the release of proinflammatory cytokines, such as interferons, which exhibit potent antiviral action; thus, protecting uninfected cells and inducing apoptosis of infected ones. Therefore, the TLR3 gene is a good candidate for the development of T1DM. Within this context, the objective of the present review was to address the role of the TLR3 gene in the development of T1DM. Arch Endocrinol Metab. 2015;59(1):4-12.
Descritores: Diabetes Mellitus Tipo 1/genética
RNA de Cadeia Dupla/metabolismo
/genética
TOLL-LIKE RECEPTOR ABATTOIRS/genética
-Citocinas/metabolismo
Diabetes Mellitus Tipo 1/imunologia
Diabetes Mellitus Tipo 1/virologia
Enterovirus/imunologia
Enterovirus/fisiologia
Imunidade Inata/fisiologia
Inflamação/metabolismo
Células Secretoras de Insulina/metabolismo
Transdução de Sinais/fisiologia
/metabolismo
TOLL-LIKE RECEPTOR ABATTOIRS/metabolismo
Replicação Viral/genética
Replicação Viral/imunologia
Limites: Animais
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Revisão
Responsável: BR1.1 - BIREME


  4 / 10 LILACS  
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Texto completo SciELO Brasil
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Id: lil-685497
Autor: Memorias do Instituto Oswaldo Cruz; Mourao, Marina de Moraes; Bitar, Maina; Lobo, Francisco Pereira; Peconick, Ana Paula; Grynberg, Priscila; Prosdocimi, Francisco; Waisberg, Michael; Cerqueira, Gustavo Coutinho; Macedo, Andrea Mara; Machado, Carlos Renato; Yoshino, Timothy; Franco, Gloria Regina.
Título: A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
Fonte: Mem. Inst. Oswaldo Cruz;108(6):707-717, set. 2013. tab, graf.
Idioma: en.
Projeto: CNPq; . FAPEMIG.
Resumo: Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of trans-spliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974).
Descritores: Técnicas de Silenciamento de Genes
Precursores de RNA/isolamento & purificação
RNA Líder para Processamento/genética
Schistosoma mansoni/genética
Trans-Splicing/fisiologia
-Etiquetas de Sequências Expressas
Biblioteca Gênica
Regulação da Expressão Gênica/genética
Larva
Estágios do Ciclo de Vida/genética
Fenótipo
Reação em Cadeia da Polimerase em Tempo Real
Precursores de RNA/genética
RNA de Cadeia Dupla
RNA Interferente Pequeno/metabolismo
Schistosoma mansoni/crescimento & desenvolvimento
Trans-Splicing/genética
Limites: Animais
Feminino
Masculino
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  5 / 10 LILACS  
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Texto completo SciELO Chile
Texto completo
Id: lil-448772
Autor: Jana, Snehasis; Chakraborty, Chiranjib; Nandi, Shyamsundar.
Título: Mechanisms and roles of the RNA-based gene silencing
Fonte: Electron. j. biotechnol;7(3):15-16, Dec. 2004. ilus, tab, graf.
Idioma: en.
Resumo: RNA silencing is a remarkable type of gene regulation. This process has been found to occur in many different organisms such as plants (co-suppression), fungi (quelling), and animals (RNA interference; RNAi). Double-stranded RNA (dsRNA) is a potent trigger in RNA silencing mechanisms operating in a wide range of organisms. This mechanism recognizes dsRNA and processes them into small 21-25nt RNAs (smRNAs). Small RNAs can guide post-transcriptional degradation of complementary messenger RNAs and in plants, transcriptional gene silencing is occurred by methylation of homologous DNA sequences. In plants, it serves as an antiviral defense, and many plant viruses encode suppressors of silencing such as helper component-proteinase of potyviruses (HC-Pro) and the p25 protein encoded by potato virus X (PVX). HC-Pro acts by preventing accumulation of smRNAs that provide specificity determinant for homologous RNA degradation, but p25 viral protein acts by targeting the mobile silencing signal. The encouraging view is that RNA silencing is part of a sophisticated network of interconnected pathways for cellular defense and development and that it may become a powerful tool to manipulate gene expression experimentally.
Descritores: RNA de Cadeia Dupla/metabolismo
Inativação Gênica
Interferência de RNA
-Metilação de DNA
Fungos
Modelos Biológicos
Plantas
Limites: Animais
Tipo de Publ: Revisão
Responsável: CL1.1 - Biblioteca Central


  6 / 10 LILACS  
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Id: lil-419686
Autor: Fraga, Jorge; Rojas, Lázara; Sariego, Idalia; Fernández-Calienes, Aymé.
Título: Double-stranded RNA viral infection in Cuban Trichomonas vaginalis isolates
Fonte: Braz. j. infect. dis;9(6):521-524, Dec. 2005. ilus.
Idioma: en.
Resumo: Trichomonas vaginalis can be infected with double-stranded RNA (dsRNA) viruses designated T. vaginalis virus (TVV), which may have important implications for trichomonal virulence and disease pathogenesis. We tested for TVV in 40 fresh T. vaginalis isolates from Cuban patients by total extraction of nucleic acids (DNA and RNA). TVV was detected in 22 (55 percent) of the 40 T. vaginalis isolates. This gives an estimate of the infection rate of Cuban T. vaginalis isolates by the dsRNA virus. Future research should focus on the association between trichomonosis symptoms and the presence of TVV.
Descritores: Vírus de RNA/isolamento & purificação
RNA de Cadeia Dupla/isolamento & purificação
Vaginite por Trichomonas/virologia
Trichomonas vaginalis/virologia
-Cuba
DNA Viral/análise
Eletroforese em Gel de Ágar
Vírus de RNA/genética
RNA de Cadeia Dupla/genética
RNA Viral/análise
Trichomonas vaginalis/isolamento & purificação
Trichomonas vaginalis/patogenicidade
Limites: Adolescente
Animais
Feminino
Humanos
Responsável: BR1.1 - BIREME


  7 / 10 LILACS  
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Id: lil-417185
Autor: Lenz, G.
Título: The RNA interference revolution
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;38(12):1749-1757, Dec. 2005. ilus.
Idioma: en.
Resumo: The discovery of double-stranded RNA-mediated gene silencing has rapidly led to its use as a method of choice for blocking a gene, and has turned it into one of the most discussed topics in cell biology. Although still in its infancy, the field of RNA interference has already produced a vast array of results, mainly in Caenorhabditis elegans, but recently also in mammalian systems. Micro-RNAs are short hairpins of RNA capable of blocking translation, which are transcribed from genomic DNA and are implicated in several aspects from development to cell signaling. The present review discusses the main methods used for gene silencing in cell culture and animal models, including the selection of target sequences, delivery methods and strategies for a successful silencing. Expected developments are briefly discussed, ranging from reverse genetics to therapeutics. Thus, the development of the new paradigm of RNA-mediated gene silencing has produced two important advances: knowledge of a basic cellular mechanism present in the majority of eukaryotic cells and access to a potent and specific new method for gene silencing.
Descritores: Caenorhabditis elegans/genética
Interferência de RNA/fisiologia
RNA Interferente Pequeno/genética
RNA de Cadeia Dupla/genética
-Técnicas de Transferência de Genes
Camundongos Knockout
Limites: Animais
Camundongos
Tipo de Publ: Revisão
Responsável: BR1.1 - BIREME


  8 / 10 LILACS  
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Id: lil-283057
Autor: Azevedo, Andréia Cristiane Souza; Sosa-Gómez, Daniel Ricardo; Faria, Marcos Rodrigues; Fungaro, Maria Helena Pelegrinelli.
Título: Effects of double-stranded RNA on virulence of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) against the silverleaf whitefly, Bemisia tabaci strain B (Homoptera: Aleyrodidae)
Fonte: Genet. mol. biol;23(1):61-3, Mar. 2000. ilus, tab.
Idioma: en.
Resumo: Bandas de dsRNA foram detectadas em três dos doze isolados de Paecilomyces fumosoroseus. A identidade destas bandas foi provada através de tratamentos com RNAse, DNAse e S1 nuclease. A cura do dsRNA para um dos isolados (P92) foi obtida através do isolamento de colônias monospóricas. Linhagens isogênicas, com e sem dsRNA, foram submetidas ao teste de virulência contra a mosca branca Bemisia tabaci biotipo B. Ao contrário do que ocorre para vários fungos fitopatogênicos, os fragmentos de dsRNA näo causaram hipovirulência em P.fumosoroseus.
Descritores: Fungos
RNA de Cadeia Dupla
-Insetos
Paecilomyces
Limites: Animais
Responsável: BR26.1 - Biblioteca Central


  9 / 10 LILACS  
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Id: lil-204019
Autor: Gatti, Maria Silvia Viccari.
Título: Psicobirnavírus: um novo vírus animal, características gerais, infecçäo experimental em ratos e estudos epidemiológicos em suínos.
Fonte: Säo Paulo; s.n; 1994. 106 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Escola Paulista de Medicina para obtenção do grau de Doutor.
Descritores: Resinas Acrílicas
Eletroforese
RNA de Cadeia Dupla
RNA Viral/análise
Limites: Ratos
Animais
Responsável: BR1.2 - Biblioteca Central
BR1.2; 1495


  10 / 10 LILACS  
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Id: lil-85154
Autor: Guimaraës, M. A. A. M; Nozawa, C. M.
Título: Acridine orange metachromasia in the cytoplasm of simian rotavirus (SA-11)-infected MA-104 cell cultures
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;23(2):169-77, 1990. tab, ilus.
Idioma: en.
Resumo: Acridine orange metachromasia was used to determine the distribution of simian rotavirus double-stranded RNA in cultured MA-104 cells 0 to 72 h post-infection. Correlations were made among time of detection and amount of viral antigens, virus yield and the ultrastructural aspects of infected cells. RNAase-resistant cytoplasmic metachromasia appeared 48 h post-infection, 36 h after the initial detection of viral antigens or infectious virions and 24 h after the appearance of the cytopathic effect. Acridine orange staining is thus useful for monitoring the progress of rotaviral infection in cell cultures due to its simplicity and low cost, in spite of its lower sensitivity compared to other techniques evaluated
Descritores: Laranja de Acridina
Antígenos Virais/análise
Infecções por Rotavirus/diagnóstico
Rotavirus/imunologia
Rotavirus/ultraestrutura
-Células Cultivadas/microbiologia
RNA de Cadeia Dupla/metabolismo
Coloração e Rotulagem
Responsável: BR26.1 - Biblioteca Central



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