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Pesquisa : D13.444.735.500 [Categoria DeCS]
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Id: lil-749738
Autor: Khan, Shamiyan R.; Nirmal, J.I. Kumar; Kumar, Rita N.; Patel, Jignasha G..
Título: Biodegradation of kerosene: Study of growth optimization and metabolic fate of P. janthinellum SDX7
Fonte: Braz. j. microbiol;46(2):397-406, Apr-Jun/2015. tab, graf.
Idioma: en.
Resumo: Penicillum janthinellum SDX7 was isolated from aged petroleum hydrocarbon-affected soil at the site of Anand, Gujarat, India, and was tested for different pH, temperature, agitation and concentrations for optimal growth of the isolate that was capable of degrading upto 95%, 63% and 58% of 1%, 3% and 5% kerosene, respectively, after a period of 16 days, at optimal growth conditions of pH 6.0, 30 °C and 180 rpm agitation. The GC/MS chromatograms revealed that then-alkane fractions are easily degraded; however, the rate might be lower for branched alkanes, n-alkylaromatics, cyclic alkanes and polynuclear aromatics. The test doses caused a concentration-dependent depletion of carbohydrates of P. janthinellum SDX7 by 3% to 80%, proteins by 4% to 81% and amino acids by 8% to 95% upto 16 days of treatment. The optimal concentration of 3% kerosene resulted in the least reduction of the metabolites of P. janthinellum such as carbohydrates, proteins and amino acids with optimal growth compared to 5% and 1% (v/v) kerosene doses on the 12th and 16th day of exposure. Phenols were found to be mounted by 43% to 66% at lower and higher concentrations during the experimental period. Fungal isolate P. janthinellum SDX7 was also tested for growth on various xenobiotic compounds.
Descritores: Querosene
Penicillium/crescimento & desenvolvimento
Penicillium/metabolismo
Microbiologia do Solo
Poluentes do Solo/metabolismo
Xenobióticos/metabolismo
-Composição de Bases
Biotransformação
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Cromatografia Gasosa-Espectrometria de Massas
Genes de RNAr
Concentração de Íons de Hidrogênio
Índia
Dados de Sequência Molecular
Penicillium/genética
Penicillium/isolamento & purificação
RNA Fúngico/genética
/genética
RNA, RIBOSOMAL, 1ABDOMINAL NEOPLASMSS/genética
Análise de Sequência de DNA
Temperatura
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  2 / 14 LILACS  
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Id: lil-730266
Autor: Fuentes, Marisol; Hermosilla, Germán; Alburquenque, Claudio; Falconer, Mary A; Amaro, José; Tapia, Cecilia.
Título: Caracterización de los mecanismos de resistencia a azoles en aislados clínicos chilenos de Candida albicans / Characterization of azole resistance mechanisms in Chilean clinical isolates of Candida albicans
Fonte: Rev. chil. infectol;31(5):511-517, oct. 2014. ilus, graf, tab.
Idioma: es.
Projeto: Fondecyt; . UCH.
Resumo: Introduction: The commensal yeast Candida albicans, can cause superficial or systemic candidiasis in susceptible hosts. In Chile, azole antifungals are the most widely used drugs in the treatment of candidiasis. In a previous study performed at our center, 2.1 and 1.6% of clinical isolates of C. albicans were found to be resistant to fluconazole and voriconazole, respectively. Objective: To characterize the resistance mechanisms involved in azoles resistance in Chilean clinical isolates. Methodology: Eight resistant, nine susceptible-dose dependent (SDD) and 10 susceptible strains (n: 27) were selected according to the Clinical Laboratory Standards Institute (CLSI) M27-S3 criteria, from vaginal and urine samples. Mutations in the 408-488 region of the ERG11 gene were studied by sequencing, and the relative expression of ERG11 gene and efflux pump genes CDR1, CDR2 and MDR1, was evaluated by quantitative real-time PCR (q-PCR). Results: No mutations were detected in the ERG11 gene and its overexpression was found only in 12.5% of the resistant strains (1/8). The most prevalent mechanism of resistance was the over-expression of efflux pumps (62.5%; 5/8). Conclusion: The study of the expression of efflux pumps by q-PCR could be a useful diagnostic tool for early detection of azole resistance in C. albicans.

Introducción: Candida albicans es una levadura comensal capaz de causar una infección oportunista en hospederos susceptibles denominada candidiasis, que puede ser superficial o sistémica. En Chile, los antifúngicos más utilizados para el tratamiento de las candidiasis son los azoles. En un estudio previo en nuestro centro, se detectó que 2,1 y 1,6% de cepas clínicas de C. albicans fueron resistentes a fluconazol y voriconazol, respectivamente. Objetivo: Caracterizar los mecanismos de resistencia involucrados en la resistencia a azoles en cepas clínicas chilenas. Metodología: Según los criterios del Clinical Laboratory Standards Institute (CLSI) M27-S3, se seleccionaron ocho cepas resistentes, nueve cepas susceptibles dosis dependiente (SDD) y 10 cepas sensibles (n: 27), aisladas de flujo vaginal y orina. Se evaluó la presencia de mutaciones en la región 408-488 del gen ERG11 por secuenciación y la expresión relativa del gen ERG11 y de los genes de bombas de eflujo CDR1, CDR2 y MDR1 por RPC en tiempo real cuantitativa (q-PCR). Resultados: No se encontraron mutaciones en el gen ERG11 y la sobre-expresión de éste sólo se presentó en 12,5% de las cepas resistentes (1/8). El mecanismo prevalente en la cepas resistentes fue la sobre-expresión de bombas de eflujo encontrándose en 62,5% de las cepas resistentes (5/8). Conclusión: El estudio de la expresión bombas de eflujo por q-PCR podría ser una herramienta diagnóstica útil para la detección temprana de resistencia a azoles en C. albicans.
Descritores: Antifúngicos/farmacologia
Candida albicans/efeitos dos fármacos
Fluconazol/farmacologia
Voriconazol/farmacologia
-Chile
Candida albicans/genética
Candida albicans/isolamento & purificação
Farmacorresistência Fúngica
Regulação Fúngica da Expressão Gênica
Genes Fúngicos/genética
Reação em Cadeia da Polimerase em Tempo Real
RNA Fúngico/genética
Limites: Feminino
Humanos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


  3 / 14 LILACS  
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Id: lil-699830
Autor: Qiu, Shanlian; Wang, MK; Wang, Fei; Chen, Jichen; Li, Xiaoyan; Li, Qinghua; Lin, Cheng; Lin, Xinjian.
Título: Effects of open drainage ditch design on bacterial and fungal communities of cold waterlogged paddy soils
Fonte: Braz. j. microbiol;44(3):983-991, July-Sept. 2013. ilus, graf, tab.
Idioma: en.
Projeto: China. Agro-scientific Research; . China. Fujian Academy of Agricultural Sciences; . China. Fujian Province.
Resumo: A field experiment established in 1980 was conducted to evaluate the effects of open drainage ditch applied for water removal on bacterial and fungal communities of cold waterlogged paddy soils in 2011. In this experiment, traditional plate counting and temperature gradient gel electrophoresis were employed to characterize the abundance and diversity of soil bacterial and fungal communities. Four different distances from the open drainage ditch, 5, 15, 25 and 75 m with different degrees of drainage were designed for this study. Maximum populations of culturable aerobic bacteria and fungi were at 15-m distance while minimum populations were at 75-m distance. Significant differences (p < 0.05) in fungal populations were observed at all distances from open drainage ditch. The highest diversity of the bacterial community was found at a distance of 25 m, while that of the fungal community was observed at a distance of 5 m. Sequencing of excised TGGE bands indicated that the dominant bacteria at 75-m distance belonged to anaerobic or microaerobic bacteria. Relationships between microbial characteristics and soil physicochemical properties indicated that soil pH and available nitrogen contents were key factors controlling the abundance of culturable aerobic bacteria and fungi, while soil water capacity also affected the diversity of fungal community. These findings can provide the references for better design and advanced management of the drainage ditches in cold waterlogged paddy soils.
Descritores: Biota
Bactérias/classificação
Bactérias/isolamento & purificação
Fenômenos Químicos
Fungos/classificação
Fungos/isolamento & purificação
Microbiologia do Solo
-Análise por Conglomerados
Temperatura Baixa
Eletroforese em Gel de Gradiente Desnaturante
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Drenagem
Genes de RNAr
Concentração de Íons de Hidrogênio
Dados de Sequência Molecular
Nitrogênio/análise
Filogenia
RNA Bacteriano/genética
RNA Fúngico/genética
/genética
RNA, RIBOSOMAL, 1ABDOMINAL NEOPLASMSS/genética
/genética
RNA, RIBOSOMAL, ABNORMALITIES, MULTIPLES/genética
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
Solo/química
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  4 / 14 LILACS  
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Id: lil-657669
Autor: Zhang, Yuejin; Qin, Yali; Guo, Lijun; Zhou, Zili; Liang, Zongsuo; Zhang, Chenlu; Guo, Hongbo.
Título: Isolation of high quality RNA from Polyporus umbellatus (Pers. ) Fries
Fonte: Electron. j. biotechnol;15(5):10-10, Sept. 2012. ilus, tab.
Idioma: en.
Projeto: Education Ministry National Higher Education Fund for Doctoral Program; . National Higher Education Fund for Basic Research.
Resumo: Background: The dried sclerotium of medicinal fungus Polyporus umbellatus (Pers.) Fries has many pharmacological functions such as diuretic and anticancer activity, in which high-content polysaccharides may play an important role. However, RNA isolation is difficult in filamentous fungi and lacking in P. umbellatus. Results: Five methods for RNA extraction from five strains collected from four provinces were assessed for their ability to recover a high-quality RNA applicable for sequence-related amplification polymorphism (SRAP) PCR and GDP-D-mannose pyrophosphorylase (GMP) gene expression profiles. Both A260/A280 and A260/A230 ratios of the best Trizol Plus + RNAiso-mate for Plant Tissue method are around 2 with a yield of 1122.00 +/- 0.21 ng ul-1. The Trizol method also showed good quality with the yield 469.60 ng ul-1. The SRAP PCR amplified clear and polymorphic bands in all five cDNA samples transcribed from RNA by using primer Me4-Em4. GMP gene fragment (1251 bp) was successfully amplified by RT-PCR, suggesting the integrity of isolated RNA. Conclusion: All these results showed that the total RNA isolated by this protocol is of sufficient quality for subsequent molecular applications.
Descritores: RNA Fúngico/isolamento & purificação
Polyporus/genética
Polyporus/química
-Reação em Cadeia da Polimerase Via Transcriptase Reversa
Responsável: CL1.1 - Biblioteca Central


  5 / 14 LILACS  
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Id: lil-553764
Autor: Basso, T. S; Pungartnik, C; Brendel, M.
Título: Low productivity of ribonucleotide reductase in Saccharomyces cerevisiae increases sensitivity to stannous chloride
Fonte: Genet. mol. res. (Online);7(1):1-6, Jan. 2008. ilus.
Idioma: en.
Resumo: Ribonucleotide reductase (RNR) of the yeast Saccharomyces cerevisiae is a tetrameric protein complex, consisting of two large and two small subunits. The small subunits Y2 and Y4 form a heterodimer and are encoded by yeast genes RNR2 and RNR4, respectively. Loss of Y4 in yeast mutant rnr4delta can be compensated for by up-regulated expression of Y2, and the formation of a small subunit Y2Y2 homodimer that allows for a partially functional RNR. However, rnr4delta mutants exhibit slower growth than wild-type (WT) cells and are sensitive to many mutagens, amongst them UVC and photo-activated mono- and bi-functional psoralens. Cells of the haploid rnr4delta mutant also show a 3- to 4-fold higher sensitivity to the oxidative stress-inducing chemical stannous chloride than those of the isogenic WT. Both strains acquired increased resistance to SnCl2 with age of culture, i.e., 24-h cultures were more sensitive than cells grown for 2, 3, 4, and 5 days in liquid culture. However, the sensitivity factor of three to four (WT/mutant) did not change significantly. Cultures of the rnr4delta mutant in stationary phase of growth always showed higher frequency of budding cells (budding index around 0.5) than those of the corresponding WT (budding index <0.1), pointing to a delay of mitosis/cytokinesis.
Descritores: Compostos de Estanho/toxicidade
Genes Fúngicos/genética
Mutagênicos/toxicidade
Ribonucleotídeo Redutases/genética
Saccharomyces cerevisiae/enzimologia
-Sobrevivência Celular
Dimerização
Haploidia
Mutação
RNA Fúngico/biossíntese
Ribonucleotídeo Redutases/química
Saccharomycetales
Sensibilidade e Especificidade
Saccharomyces cerevisiae/citologia
Saccharomyces cerevisiae/genética
Fatores de Tempo
Responsável: BR26.1 - Biblioteca Central


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Id: lil-490636
Autor: Niklitschek, Mauricio; Alcaíno, Jennifer; Barahona, Salvador; Sepúlveda, Dionisia; Lozano, Carla; Carmona, Marisela; Marcoleta, Andrés; Martínez, Claudio; Lodato, Patricia; Baeza, Marcelo; Cifuentes, Víctor.
Título: Genomic organization of the structural genes controlling the astaxanthin biosynthesis pathway of Xanthophyllomyces dendrorhous
Fonte: Biol. Res;41(1):93-108, 2008. ilus, tab.
Idioma: en.
Projeto: Fondecyt; . Mecesup.
Resumo: The cloning and nucleotide sequence of the genes (idi, crtE, crtYB, crtl and crtS) controlling the astaxanthin biosynthesis pathway of the wild-type ATCC 24230 strain of Xanthophyllomyces dendrorhous in their genomic and cDNA version were obtained. The idi, crtE, crtYB, crtl and crtS genes were cloned, as fragments of 10.9, 11.5, 15.8, 5.9 and 4 kb respectively. The nucleotide sequence data analysis indicates that the idi, crtE, crtYB, crtl and crtS genes have 4, 8,4, 11, and 17 introns and 5, 9, 5, 12 and 18 exons respectively. In addition, a highly efficient site-directed mutagenesis system was developed by transformation by integration, followed by mitotic recombination (the double recombinant method). Heterozygote idi (idi+ / idi-::hph), crtE (crtE+ / crtE -::hph), crtYB (crtYB + / crtYB -::hph), crtI (crtI+ / crtI-::hph) and crtS (crtS +/crtS -::hph) and homozygote mutants crtYB (crtYB -::hph/crtYB -::hph), crtI (crtI -::hph/crtI -::hph) and crtS (crtS -::hph / crtS -::hph) were constructed. All the heterozygote mutants have a pale phenotype and produce less carotenoids than the wild-type strain. The genetic analysis of the crtYB, crtl and crtS loci in the wild-type, heterozygote, and homozygote give evidence of the diploid constitution of ATCC 24230 strains. In addition, the cloning of a truncated form of the crtYB that lacks 153 amino acids of the N-terminal region derived from alternatively spliced mRNA was obtained. Their heterologous expression in Escherichia coli carrying the carotenogenic cluster of Erwinia uredovora result in trans-complementation and give evidence of its functionality in this bacterium, maintaining its phytoene synthase activity but not the lycopene cyclase activity.
Descritores: Basidiomycota/genética
Regulação Fúngica da Expressão Gênica/genética
-Sequência de Aminoácidos
Sequência de Bases
Clonagem Molecular
DNA Complementar/genética
Genes Fúngicos/genética
Reação em Cadeia da Polimerase
RNA Fúngico/genética
Xantofilas/biossíntese
Xantofilas/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  7 / 14 LILACS  
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Id: lil-476862
Autor: Valenzuela F., Eduardo; Toro Z., Viviana; Martínez V., Oscar; Pinochet T., Dante.
Título: Determinación de fosfatasas en hongos de praderas / Phosphatases determination in praire fungi
Fonte: Bol. micol;20:83-89, dic. 2005. tab.
Idioma: es.
Resumo: Se realizó un estudio para determinar la actividad de la fosfatasa ácida y alcalina en 180 cepas fúngicas aisladas desde suelo rizósferico y la rizósfera de tres plantas forrajeras (Dactilys glomerata, Lolium perenne y Trifollium repens) cultivadas en una pradera en rotación y una pradera permanente. Las fosfatasas fueron determinadas por el método desarrollado por Tabatabai & Bremner (1969) y se leyeron en un espectrofotómetro a 400 nm. Los resultados obtenidos se sometieron a un análisis de varianza (ANDEVA). En las cepas fúngicas ensayadas se determinó actividad para fosfatasa ácida y alcalina. Los mayores valores tanto para fosfatasa ácida (139.68 mg/mL*g micelio) y alcalina (127.12 mg/mL*g micelio) los registró la cepa 14-2R de Penicillium chrysogenum aislada de la rizófera de L. perenne cultivado en pradera permanente. Entre las mejores cepas evaluadas de la pradera permanente existió una mejor concordancia entre los valores determinados para fosfatasa ácida y alcalina.
Descritores: Fosfatase Ácida/análise
Fosfatase Ácida/fisiologia
Fosfatase Alcalina/análise
Fosfatase Alcalina/fisiologia
Fungos/isolamento & purificação
Penicillium chrysogenum/isolamento & purificação
-Chile
DNA Fúngico
RNA Fúngico
Microbiologia do Solo
Qualidade do Solo
Responsável: CL2.1 - Biblioteca de Medicina


  8 / 14 LILACS  
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Id: lil-464302
Autor: Gao, L; Sun, Y; Chen, C; Xi, Y; Wang, J; Wang, Z.
Título: Primary mechanism of apoptosis induction in a leukemia cell line by fraction FA-2-b-ß prepared from the mushroom Agaricus blazei Murill
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;40(11):1545-1555, Nov. 2007. ilus, tab.
Idioma: en.
Resumo: Agaricus blazei Murill is a native Brazilian mushroom which functions primarily as an anticancer substance in transplanted mouse tumors. However, the mechanism underlying this function of A. blazei Murill remains obscure. The present study was carried out to investigate the effect of fraction FA-2-b-ß, an RNA-protein complex isolated from A. blazei Murill, on human leukemia HL-60 cells in vitro. Typical apoptotic characteristics were determined by morphological methods using DNA agarose gel electrophoresis and flow cytometry. The growth suppressive effect of fraction FA-2-b-ß on HL-60 cells in vitro occurred in a dose- (5-80 mug/mL) and time-dependent (24-96 h) manner. The proliferation of HL-60 cells (1 x 10(5) cells/mL) treated with 40 mug/mL of fraction FA-2-b-ß for 24-96 h and with 5-80 mug/mL for 96 h resulted in inhibitory rates ranging from 8 to 54.5 percent, and from 4.9 to 86.3 percent, respectively. Both telomerase activity determined by TRAP-ELISA and mRNA expression of the caspase-3 gene detected by RT-PCR were increased in HL-60 cells during fraction FA-2-b-ß treatment. The rate of apoptosis correlated negatively with the decrease of telomerase activity (r = 0.926, P < 0.05), but correlated positively with caspase-3 mRNA expression (r = 0.926, P < 0.05). These data show that fraction FA-2-b-ß can induce HL-60 cell apoptosis and that the combined effect of down-regulation of telomerase activity and up-regulation of mRNA expression of the caspase-3 gene could be the primary mechanism of induction of apoptosis. These findings provide strong evidence that fraction FA-2-b-ß could be of interest for the clinical treatment of acute leukemia.
Descritores: Agaricus/química
Antineoplásicos/farmacologia
Apoptose/efeitos dos fármacos
RNA Fúngico/química
Proteínas de Ligação a RNA/farmacologia
-Antineoplásicos/isolamento & purificação
/análise
CASPASE ABATTOIRS/análise
Proliferação de Células/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Eletroforese em Gel de Ágar
/efeitos dos fármacos
HL-ACCESSORY NERVE CELLS/efeitos dos fármacos
Microscopia Eletrônica de Transmissão
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Fúngico/isolamento & purificação
RNA Mensageiro/química
Proteínas de Ligação a RNA/isolamento & purificação
Fatores de Tempo
Telomerase/análise
Limites: Humanos
Responsável: BR1.1 - BIREME


  9 / 14 LILACS  
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Id: lil-456610
Autor: Lodato, P; Alcaíno, J; Barahona, S; Niklitschek, M; Carmona, M; Wozniak, A; Baeza, M; Jiménez, A; Cifuentes, V.
Título: Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous
Fonte: Biol. Res;40(1):73-84, 2007. graf, tab.
Idioma: en.
Projeto: Fondecyt.
Resumo: In the yeast Xanthophyllomyces dendrorhous the genes idi, crtE, crtYB, crtl and ast are involved in the biosynthesis of astaxanthin from isopentenyl pyrophosphate. The carotenoid production and the kinetics of mRNA expression of structural genes controlling the carotenogenesis in a wild-type ATCC 24230 and in carotenoid overproducer deregulated atxS2 strains were studied. The biosynthesis of carotenoid was induced at the late exponential growth phase in both strains. However, the cellular carotenoid concentration was four times higher in atxS2 than in the wild-type strain in the exponential growth phase, suggesting that carotenogenesis was deregulated in atxS2 at the beginning of growth. In addition, the maximum expression of the carotenogenesis genes at the mRNA level was observed during the induction period of carotenoid biosynthesis in the wild-type strain. The mRNA level of the crtYB, crtl, ast genes and to a lesser extent the idi gene, decayed at the end of the exponential growth phase. The mRNA levels of the crtE gene remained high along the whole growth curve of the yeast. In the atxS2 strain the mRNA levels of crtE gene were about two times higher than the wild-type strain in the early phase of the growth cycle.
Descritores: Basidiomycota/genética
Carotenoides/genética
Regulação Fúngica da Expressão Gênica
-Basidiomycota/metabolismo
Meios de Cultura
Carotenoides/biossíntese
Reação em Cadeia da Polimerase Via Transcriptase Reversa
RNA Fúngico/genética
RNA Mensageiro/genética
Xantofilas
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


  10 / 14 LILACS  
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Id: lil-445288
Autor: Albuquerque, P; Baptista, A. J; Derengowsky, L. da S; Procópio, L; Nicola, A. M; Arraes, F. B; Souza, D. P; Kyaw, C. M; Silva-Pereira, I.
Título: Paracoccidioides brasiliensis RNA biogenesis apparatus revealed by functional genome analysis
Fonte: Genet. mol. res. (Online);4(2):251-272, 30 jun. 2005. tab.
Idioma: en.
Resumo: The RNA biogenesis machinery of Paracoccidioides brasiliensis was assessed by comparative analyses of PbAESTs (P. brasiliensis assembled expressed sequence tags (ESTs)) with sequences from Saccharomyces cerevisiae MIPS database. PbAESTs related to almost all categories of S. cerevisiae RNA biogenesis were found. Two of the 12 S. cerevisiae RNA Pol II core subunits, Rpb3 and Rpb7, were found, probably reflecting the growth phase from which the cDNA libraries used in ESTs generation were constructed, as well as the low abundance of some of these transcripts. We have also found orthologs to TATA-box-binding protein (TBP), and at least one subunit of each TBP-associated factors (TFII) in P. brasiliensis transcriptome, except TFIIB. Genes associated to the chromatin remodeling complex, as well as transcription factors probably involved in the control of genes associated to a sexual cycle and virulence, were also identified. With respect to the pre-mRNA processing, 65 PbAEST orthologs to S. cerevisiae basal splicing machinery and 21 orthologs of 5'- and 3'-end formation processes were found. Components involved in RNA interference were detected, suggesting that this gene expression regulation mechanism is probably used by P. brasiliensis. Twelve PbAESTs related to Pol I and Pol III machineries were assigned as S. cerevisiae orthologs. Finally, 25 and 10 PbAESTs associated to rRNA and tRNA processing, respectively, were detected. Taken together, our results enable us to depict, for the first time, a global view of transcription and RNA processing in P. brasiliensis.
Descritores: Origem da Vida
Etiquetas de Sequências Expressas
Fatores de Transcrição/genética
Paracoccidioides/genética
-Fatores de Transcrição/fisiologia
Genoma Fúngico
Paracoccidioides/fisiologia
Reprodução
RNA Fúngico/genética
RNA Polimerase II/genética
RNA Polimerase II/fisiologia
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/fisiologia
Transcrição Genética/fisiologia
Limites: Humanos
Responsável: BR1.1 - BIREME



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