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Chaib, Eleazar
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Id: biblio-949203
Autor: Vasques, Enio Rodrigues; Cunha, José Eduardo Monteiro; Kubrusly, Marcia Saldanha; Coelho, Ana Maria; Sanpietri, Sandra N; Nader, Helena B; Tersariol, Ivarne L S; Lima, Marcelo A; Chaib, Eleazar; D'Albuquerque, Luiz Augusto Carneiro.
Título: The m-rna, expression of serca2 and ncx1 in the process of pharmacological cell protection in experimental acute pancreatitis induced by taurocholate / Expressão do rna-m, da serca2 e do ncx1 no processo de proteção celular farmacológica na pancreatite aguda experimental induzida por taurocolato
Fonte: ABCD arq. bras. cir. dig;31(1):e1352, 2018. tab.
Idioma: en.
Resumo: ABSTRACT Background: Intracellular calcium overload is known to be a precipitating factor of pancreatic cell injury in acute pancreatitis (AP). Intracellular calcium homeostasis depends of Plasmatic Membrane Calcium ATPase (PMCA), Sarcoplasmic Endothelial Reticulum Calcium ATPase 2 (SERCA 2) and the Sodium Calcium Exchanger (NCX1). The antioxidant melatonin (Mel) and Trisulfate Disaccharide (TD) that accelerates NCX1 action could reduce the cell damage determined by the AP. Aim: To evaluate m-RNA expressions of SERCA2 and NCX1 in acute pancreatitis induced by sodium taurocholate in Wistar rats pre-treated with melatonin and/or TD. Methods: Wistar rats were divided in groups: 1) without AP; 2) AP without pre-treatment; 3) AP and Melatonin; 4) AP and TD; 5) AP and Melatonin associated to TD. Pancreatic tissue samples were collected for detection of SERCA2 and NCX1 m-R NA levels by polymerase chain reaction (PCR). Results: Increased m-RNA expression of SERCA2 in the melatonin treated group, without increase of m-RNA expression of the NCX1. The TD did not affect levels of SERCA2 and NCX1 m-RNA expressions. The combined melatonin and TD treatment reduced the m-RNA expression of SERCA2. Conclusions: The effect of melatonin is restricted to increased m-RNA expression of SERCA2. Although TD does not affect gene expression, its action in accelerating calcium exchanger function can explain the slightest expression of SERCA2 m-RNA when associated with Melatonin, perhaps by a joint action of drugs with different and but possibly complementary mechanisms.

RESUMO Racional: A lesão celular da pancreatite aguda (PA) envolve sobrecarga de cálcio, regulada pela atividade da Cálcio ATPase de membrana (PMCA), Cálcio ATPase do Retículo (SERCA2) e pelo Trocador Sódio Cálcio (NCX1). A melatonina (antioxidante) e o Dissacarídeo Trissulfatado (acelerador do NCX1) poderiam reduzir a lesão celular na PA. Objetivo: Avaliar a expressão do RNAm da SERCA2 e NCX1 em modelo animal de pancreatite aguda tratados com melatonina e/ou dissacarídeo trissulfatado (DT). Método: Ratos Wistar foram divididos em grupos: 1) sem pancreatite aguda; 2) com pancreatite aguda por taurocolato; 3) PA e Melatonina; 4) PA e DT; 5) PA e Melatonina com DT. Amostras de tecido foram colhidas para detecção dos níveis de RNAm da SERCA2 e NCX1 por PCR. Resultados: Houve aumento da expressão do RNAm da SERCA2 no grupo com PA tratados com Melatonina, porém sem aumento de expressão do NCX1. O DT não afetou os níveis de SERCA2 e NCX1. O tratamento conjunto com Melatonina e DT diminuiu a expressão da SERCA2. Conclusões: O efeito da Melatonina é restrito ao aumento da expressão da SERCA2. O DT não tem ação na expressão gênica, porém sua ação na aceleração do trocador na retirada do cálcio pode explicar a menor expressão da SERCA2 quando associado à Melatonina, pela ação conjunta de drogas com mecanismos diferentes e possivelmente complementares.
Descritores: Pancreatite/genética
RNA Mensageiro/biossíntese
Trocador de Sódio e Cálcio/genética
Citoproteção/genética
ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático/genética
-Pancreatite/induzido quimicamente
Ácido Taurocólico/administração & dosagem
Doença Aguda
Ratos Wistar
Dissacarídeos/farmacologia
Modelos Animais de Doenças
Melatonina/farmacologia
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Mantovani, Mário Sérgio
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Id: biblio-1283485
Autor: Biazi, Bruna Isabela; Zanetti, Thalita Alves; Marques, Lilian Areal; Baranoski, Adrivanio; Coatti, Giuliana Castello; Mantovani, Mário Sérgio.
Título: mRNAs biomarker related to the control of proliferation and cell death in HepG2/C3A spheroid and monolayer cultures treated with piperlongumine
Fonte: Appl. cancer res;40:1-13, Oct. 19, 2020. ilus.
Idioma: en.
Resumo: Background: Cell culture (spheroid and 2D monolayer cultures) is an essential tool in drug discovery. Piperlongumine (PLN), a naturally occurring alkaloid present in the long pepper (Piper longum), has been implicated in the regulation of GSTP1 activity. In vitro treatment of cancer cells with PLN increases ROS (reactive oxygen species) levels and induces cell death, but its molecular mode of action has not been entirely elucidated. Methods: In this study, we correlated the antiproliferative effects (2D and 3D cultures) of PLN (CAS 20069­09-4, Sigma-Aldrich) with morphological and molecular analyses in HepG2/C3A cell line. We performed assays for cytotoxicity (MTT), comet assays for genotoxicity, induction of apoptosis, analysis of the cell cycle phase, and analysis of the membrane integrity by flow cytometry. Relative expression of mRNA of genes related to proliferation, apoptosis, cell cycle control, metabolism of xenobiotics, and reticulum endoplasmic stress. Results: PLN reduced the cell proliferation by the cell cycle arrest in G2/M. Changes in the mRNA expression for CDKN1A (4.9x) and CCNA2 (0.5x) of cell cycle control genes were observed. Cell death occurred due to apoptosis, which may have been induced by increased expression of proapoptotic mRNAs (BAK1, 3.1x; BBC3, 2.4x), and by an increase in 9 and 3/7 active caspases. PLN induced cellular injury by ROS generation and DNA damage. DNA damage induced MDM2 signaling (3.0x) associated with the appearance of the monastral spindle in mitosis. Genes associated with ROS degradation also showed increased mRNA expression (GSR, 2.0x; SOD1, 2.1x). PLN induce endoplasmic reticulum stress with the increase in the mRNA expression of ERN1 (4.5x) and HSPA14 (2.2x). The xenobiotic metabolism showed increased mRNA expression for CYP1A2 (2.2x) and CYP3A4 (3.4x). In addition to 2D culture, PLN treatment also inhibited the growth of 3D culture (spheroids). Conclusion: Thus, the findings of our study show that several gene expression biomarkers (mRNAs) and monastral spindle formation indicated the many pathways of damage induced by PLN treatment that contributes to its antiproliferative effects
Descritores: RNA Mensageiro/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
Técnicas de Cultura de Células
Proliferação de Células/efeitos dos fármacos
Dioxolanos/farmacologia
Antineoplásicos/farmacologia
-Biomarcadores/análise
Expressão Gênica/efeitos dos fármacos
Esferoides Celulares/efeitos dos fármacos
Células Hep G2/efeitos dos fármacos
Limites: Humanos
Responsável: BR30.1 - Biblioteca


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Id: biblio-1278914
Autor: Barros-Oliveira, Maria da Conceição; Costa-Silva, Danylo Rafhael; dos Santos, Alesse Ribeiro; Pereira, Renato Oliveira; Soares-Júnior, José Maria; Silva, Benedito Borges da.
Título: Influence of CYP19A1 gene expression levels in women with breast cancer: a systematic review of the literature
Fonte: Clinics;76:e2846, 2021. tab, graf.
Idioma: en.
Resumo: Breast cancer is the most frequently diagnosed malignant neoplasm in women and is considered a multifactorial disease of unknown etiology. One of the major risk factors is genetic alteration. Changes in CYP19A1 gene expression levels have been associated with increased risk and increased aggressiveness of breast cancer. Increased CYP19A1 gene expression and/or aromatase activity are among the major regulatory events for intratumoral production of estrogens in breast malignant tissues. This systematic review aimed to investigate the influence of CYP19A1 gene expression levels in women with breast cancer. The research was carried out using the PubMed, Scopus, and Web of Science databases. Searches were conducted between February 2 and May 15, 2019. Inclusion criteria were studies published between 2009 and 2019, English language publications, and human studies addressing the gene expression of CYP19A1 in breast cancer. A total of 6.068 studies were identified through PubMed (n=773), Scopus (n=2,927), and the Web of Science (n=2,368). After selecting and applying the inclusion and exclusion criteria, six articles were included in this systematic review. This systematic review provides evidence that increased or decreased levels of CYP19A1 gene expression may be related to pathological clinical factors of disease, MFS, OS, DFS, WATi, markers of metabolic function, concentrations of E1, FSH, and in the use of multiple exons 1 of the CYP19A1 gene in breast cancer.
Descritores: Neoplasias da Mama/genética
-RNA Mensageiro
Aromatase/genética
Expressão Gênica
Estrogênios
Limites: Humanos
Feminino
Tipo de Publ: Revisão Sistemática
Responsável: BR1.1 - BIREME


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Id: biblio-1223233
Autor: Zeng, Jie; Zhou, Shiwei; Yang, Yuxin; Du, Junli; Kang, Danju; Wang, Xiaolong; Chen, Yulin.
Título: Effect of dietary nutrition on tail fat deposition and evaluation of tail-related genes in fat-tailed sheep
Fonte: Electron. j. biotechnol;46:30-37, jul. 2020. tab, graf.
Idioma: en.
Projeto: Tan Sheep Breeding Project of Ningxia; . China Agriculture Research System.
Resumo: BACKGROUND: The effects of dietary nutrition on tail fat deposition and the correlation between production performance and the Hh signaling pathway and OXCT1 were investigated in fat-tailed sheep. Tan sheep were fed different nutritional diets and the variances in tail length, width, thickness and tail weight as well as the mRNA expression of fat-related genes (C/EBPα, FAS, LPL, and HSL) were determined in the tail fat of sheep at three different growth stages based on their body weight. Furthermore, the correlations between tail phenotypes and the Hedgehog (Hh) signaling pathway components (IHH, PTCH1, SMO, and GLI1) and OXCT1 were investigated. RESULTS: C/EBPα, FAS, LPL, and HSL were expressed with differences in tail fat of sheep fed different nutritional diets at three different growth stages. The results of the two-way ANOVA showed the significant effect of nutrition, stage, and interaction on gene expression, except the between C/EBPα and growth stage. C/EBPα, FAS, and LPL were considerably correlated with the tail phenotypes. Furthermore, the results of the correlation analysis demonstrated a close relationship between the tail phenotypes and Hh signaling pathway and OXCT1. CONCLUSIONS: The present study demonstrated the gene-level role of dietary nutrition in promoting tail fat deposition and related tail fat-related genes. It provides a molecular basis by which nutritional balance and tail fat formation can be investigated and additional genes can be identified. The findings of the present study may help improve the production efficiency of fat-tailed sheep and identify crucial genes associated with tail fat deposition.
Descritores: Cauda/metabolismo
Ovinos/genética
Tecido Adiposo
Dieta
-Fenótipo
RNA Mensageiro
Coenzima A-Transferases
Expressão Gênica
Distribuição da Gordura Corporal
Adipogenia
Lipogênese/genética
Proteínas Hedgehog/genética
Reação em Cadeia da Polimerase em Tempo Real
Limites: Animais
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1045758
Autor: Yu, X; Zuo, Q; Chao, W; Jiang, R.
Título: The changes of muscle FGF-6 expression at different time points after exercise-induced muscle damage / Cambios de expresión de FGF-6 muscular en diferentes puntos del tiempo después del daño muscular inducido por ejercicio
Fonte: West Indian med. j;62(9):793-798, Dec. 2013. ilus, graf, tab.
Idioma: en.
Projeto: National Natural Science Foundation of China.
Resumo: OBJECTIVE: To investigate the changes of fibroblast growth factor (FGF)-6 expression in the regeneration and repair process after exercise-induced muscle damage (EIMD) and the relationship with skeletal muscle regeneration and repair. METHODS: The expression of FGF-6 at different time points was examined by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry staining after a downhill treadmill exercise. Skeletal muscle injury and regeneration at different times after EIMD was assessed by haematoxylin and eosin (H & E) staining. RESULTS: The FGF-6 protein expression was initially elevated, followed by a gradual reduction, while the changes of FGF-6 mRNA were almost all raised after the treadmill exercise. CONCLUSION: The results point out that FGF-6 is closely related to skeletal muscle regeneration and repair, probably implying a dual function in muscle regeneration.

OBJETIVO: Investigar los cambios de expresión del factor de crecimiento fibroblástico (FGF)-6 en el proceso de regeneración y reparación después de daño muscular inducido por el ejercicio (DMIE) y la relación con la reparación y regeneración del músculo esquelético. MÉTODOS: La expresión de FGF-6 en diferentes tiempos fue examinada mediante reacción en cadena de la polimerasa con transcriptasa inversa (RT-PCR) y tinción inmunohistoquímica, después de un ejercicio de carrera descendente en cinta rodante. La lesión del músculo esquelético y la regeneración en diferentes momentos después del DMIE, fueron evaluadas mediante hematoxilina y eosina (H & E). RESULTADOS: La expresión de la proteína FGF-6 fue elevada al principio, seguida por una reducción gradual, mientras que los cambios de FGF-6 mRNA fueron casi todos incrementados tras los ejercicios en la cinta rodante. CONCLUSIÓN: Nuestros resultados señalan que FGF-6 se relaciona estrechamente con la regeneración del músculo esquelético, lo que probablemente implica una función dual en la regeneración muscular.
Descritores: Condicionamento Físico Animal/efeitos adversos
Regeneração/fisiologia
Músculo Esquelético/lesões
Fator 6 de Crescimento de Fibroblastos/metabolismo
-RNA Mensageiro/metabolismo
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Modelos Animais de Doenças
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-973461
Autor: di Filippo Iriarte, Giselle; Orjuela Vargas, Julie Liliana; Osorio Zambrano, William Frend; Jiménez Camargo, Laura Carolina.
Título: Detección de ARNm de oncoproteínas E6/E7 del Virus del Papiloma Humano en cáncer de cuello uterino / Detection of Human Papillomavirus E6/E7 mRNA in cervical cancer / Detecção de RNAm de oncoproteínas E6/E7 do Vírus do Papiloma Humano em câncer de colo de útero
Fonte: Acta bioquím. clín. latinoam;52(3):361-372, set. 2018. ilus, graf, map, tab.
Idioma: es.
Resumo: La infección de transmisión sexual por el Virus del Papiloma Humano (VPH) es una de las más comunes en el mundo. Los VPH se clasifican en bajo riesgo y alto riesgo. Los VPH de bajo riesgo son los causantes de lesiones benignas como verrugas genitales y condilomas acuminados, mientras que los VPH de alto riesgo pueden originar la transformación maligna de las células del epitelio cervical por la acción de las oncoproteínas E6 y E7, lo que puede dar origen al cáncer de cuello uterino. La detección de los niveles de ARN mensajero (ARNm) de E6 y E7 de VPH en las células del epitelio cervical está siendo evaluada como un marcador predictivo del cáncer de cuello uterino como alternativa a las técnicas de detección del ADN de VPH. En este artículo se realizó una revisión crítica acerca de la relación existente entre la detección de la expresión de ARNm de E6 y E7 del virus del papiloma humano y el cáncer de cuello uterino. Además, se revisaron las técnicas aprobadas actualmente por la FDA (Food and Drug Administration) para la detección de ARNm de las oncoproteínas E6 y E7 de VPH y el estado del arte del VPH en Colombia.

The sexually transmitted infection by the Human Papillomavirus (HPV) is one of the most common in the world. Human papillomaviruses are classified as low risk and high risk. Low-risk HPVs are the cause of benign lesions such as genital warts and condylomata acuminata, while high-risk HPV types can cause malignant transformation of cervical epithelial cells by the action of oncoproteins E6 and E7, which could result in the development of cervical cancer. The detection of levels of HPV E6 and E7 mRNA (messenger RNA) in cervical epithelial cells is being evaluated as a predictive marker of cervical cancer as an alternative to HPV DNA detection techniques. In this article, a critical review was made about the relationship between the detection of E6 and E7 mRNA expression of human papillomavirus and cervical cancer. Furthermore, the techniques currently approved by the Food and Drug Administration (FDA) for the detection of mRNA from the HPV E6 and E7 oncoproteins and the state of the art of HPV in Colombia were reviewed.

A infecção sexualmente transmissível pelo Vírus do Papiloma Humano (HPV) é uma das mais comuns no mundo. Os HPV são classificados em tipos de baixo e de alto risco. Os HPV de baixo risco são a causa de lesões benignas, tais como verrugas genitais e condilomas acuminados, enquanto que os HPV de alto risco podem causar a transformação maligna das células do epitélio cervical pela ação das oncoproteínas E6 e E7, o que pode dar origem ao câncer de colo de útero. A detecção dos níveis de RNA Mensageiro (RNAm) E6 e E7 de HPV nas células do epitélio cervical está sendo avaliada como marcador preditivo do câncer de colo de útero como alternativa às técnicas de detecção de DNA do HPV. Neste artigo, foi realizada uma revisão crítica sobre a relação entre a detecção da expressão de RNAm E6 e E7 do vírus do papiloma humano e o câncer do colo de útero. Do mesmo modo, foram revisadas as técnicas aprovadas pela FDA (Food and Drug Administration) para detecção de ARNm das oncoproteínas E6 e E7 de HPV e o estado da arte do HPV na Colômbia.
Descritores: Doenças Sexualmente Transmissíveis
Neoplasias do Colo do Útero
Proteínas Oncogênicas
Biologia Celular
Alphapapillomavirus
-Virologia
DNA
RNA Mensageiro
Condiloma Acuminado
Causalidade
Células Epiteliais
Neoplasias
Limites: Humanos
Responsável: AR144.1 - CIBCHACO - Centro de Información Biomedica del Chaco


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Texto completo SciELO Chile
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Id: biblio-990038
Autor: Shati, Ali A.
Título: Sub-chronic administration of vincristine sulfate induces renal damage and apoptosis in rats via induction of oxidative stress and activation of Raf1-MEK1/2-Erk1/2 signal transduction / La administración subcrónica de sulfato de vincristina induce daño renal y apoptosis en ratas por inducción de estrés oxidativo y activación de la transducción de señales Raf1-MEK1/2-Erk1/2
Fonte: Int. j. morphol;37(1):273-283, 2019. tab, graf.
Idioma: en.
Resumo: SUMMARY: In spite of being one of the most powerful anti-cancer drug, the nephrotoxicity of Vincristine (VCR) is not well established in either animals or humans. Hence, this study evaluates the nephrotoxic effect of VCR in rats after sub-chronic long-term administration. Rats were divided into 2 groups (n=10/group) of either control and VCR treated rats (50 mg/kg). Treatments were carried out for 30 consecutive days, after which a series of biochemical and molecular experiments related to kidney function were evaluated. VCR administration significantly decreased the survival rate (69.8 %) and impaired renal function as evidenced by lowered creatinine (Cr) clearance (Ccr), high serum levels of urea and Cr, increased urinary protein levels and resulted in sever cortex pathological alterations, including glomerulus congestion and damage as well as vascular degenerations up to necrosis of both proximal and distal convoluted tubules. Mechanistically, VCR lowered renal antioxidant potential and ATP levels, enhanced lipid peroxidation and induced inflammation. In addition, VCR induced activation of Raf-1-MEK1/2-ERK1/2 signaling pathway leading to downregulation of Bcl2 and upregulation of P53, Bax, and cleaved caspase-3. In conclusion, these findings show a nephrotoxic effect of VCR sulfate in rats after sub-chronic administration and such effect was mediated by activation of ERK1/2 induced apoptosis.

RESUMEN: A pesar de ser uno de los medicamentos de mayor eficacia contra el cáncer, aún no se ha establecido la nefrotoxicidad de la vincristina (VCR) en animales y humanos. Por lo tanto, este estudio evalúa el efecto nefrotóxico de la VCR en ratas después de la administración subcrónica a largo plazo. Las ratas se dividieron en 2 grupos (n = 10 / grupo) de control y ratas tratadas con VCR (50 mg / kg). Los tratamientos se llevaron a cabo durante 30 días consecutivos, después de los cuales se evaluaron una serie de experimentos bioquímicos y moleculares relacionados con la función renal. La administración de VCR disminuyó significativamente la tasa de supervivencia (69,8 %), dificultó la función renal, lo que se observó además en los bajos niveles de creatinina (Cr) (Ccr), los niveles séricos elevados de urea y Cr, un nivel más alto de proteína urinaria, los que dieron lugar a alteraciones patológicas severas de la corteza, incluido el glomérulo congestión y daño, como también degeneraciones vasculares, incluyendo la necrosis de los túbulos contorneados proximales y distales. Mecánicamente, el VCR redujo el potencial antioxidante renal y los niveles de ATP, mejoró la peroxidación lipídica y la inflamación inducida. Además, la VCR indujo la activación de la vía de señalización Raf-1-MEK1 / 2-ERK1 / 2 que conduce a la regulación negativa de Bcl-2 y la regulación positiva de P53, Bax y la caspasa-3. En conclusión, estos hallazgos muestran un efecto nefrotóxico del sulfato de VCR en ratas después de la administración subcrónica. Dicho efecto fue mediado por la activación de la apoptosis inducida por ERK1 / 2.
Descritores: Vincristina/toxicidade
Nefropatias/induzido quimicamente
-Ureia/sangue
RNA Mensageiro
Western Blotting
Taxa de Sobrevida
Ratos Wistar
Apoptose/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Creatinina/sangue
Sistema de Sinalização das MAP Quinases
MAP Quinase Quinase 1
Proteína Quinase 3 Ativada por Mitógeno
Rim/efeitos dos fármacos
Rim/patologia
Necrose
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Texto completo SciELO Brasil
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Id: biblio-1131595
Autor: Jiang, Bo; Shi, Chun-Sheng.
Título: Dynamic changes of periostin and collagen I in the sclera during progressive myopia in guinea pigs / Alterações dinâmicas de periostina e colágeno I na esclera durante miopia progressiva em cobaias
Fonte: Arq. bras. oftalmol;83(3):190-195, May-June 2020. tab, graf.
Idioma: en.
Resumo: ABSTRACT Purpose: To investigate periostin and collagen I expression during a scleral remodeling in myopic eyes and to determine their role in collagen remodeling of the myopic sclera. Methods: Fifty one-week-old guinea pigs were divided into the control and form-deprivation myopia (FDM) groups. The eyes of animals in the form-deprivation myopia group were covered for 2, 4, and 8 weeks, or were covered for 4 weeks and then uncovered for 2 weeks. The diopters and axial lengths in the eyes in each group of guinea pigs were measured. Immunohistochemistry and reverse transcription polymerase chain reaction were used to detect the relative protein and mRNA expressions of periostin and collagen I in the scleral tissues of guinea pig. Results: Before masking, guinea pigs in the control and form-deprivation myopia groups were hypermetropic and did not differ significantly (p>0.05). Hypermetropic refraction in the control group gradually decreased. In guinea pigs from the form-deprivation myopia group, the refractive power gradually changed from +2.14 ± 0.33 D to -7.22 ± 0.51 D, and the axial length gradually changed from 5.92 ± 0.37 mm to 8.05 ± 0.34 mm from before until the end of masking. Before covering, no significant difference was observed in the relative collagen I and periostin mRNA and protein expression levels in the sclera of the guinea pig control and form-deprivation myopia groups (p>0.05). The relative collagen I and periostin protein and mRNA expression levels in the sclera of guinea pigs in the form-deprivation myopia group at 2, 4, and 8 weeks, and after covering the eyes for 4 weeks followed by uncovering for 2 weeks, were significantly lower than those in the control group (p<0.05). The collagen I and periostin mRNA expression levels were positively correlated with protein expression levels in the sclera of guinea pigs (protein: r=0.936, p<0.05; mRNA: r=0.909, p<0.05). Conclusions: Periostin was expressed in the myopic sclera of guinea pigs, and changes in periostin and collagen I expression were highly consistent. Periostin and collagen I may be involved in the regulation of scleral remodeling in myopia.

RESUMO Objetivo: Investigar a expressão da periostina e do colágeno I durante o remodelamento escleral em olhos míopes e determinar seu papel na remodelação do colágeno da esclera miópica. Métodos: Cinquenta cobaias com uma semana de idade foram divididas em grupo controle e miopia de privação de forma. Os olhos dos animais no grupo de miopia de privação de forma foram cobertos por 2, 4 e 8 semanas, ou foram cobertos por 4 semanas e depois descobertas por 2 semanas. As dioptrias e comprimentos axiais dos olhos em cada grupo de cobaias foram medidos. A imunohistoquímica e a reação em cadeia da polimerase com transcrição reversa foram utilizadas para detectar as expressões relativas de proteína e mRNA de periostina e colágeno I em tecidos esclerais das cobaias. Resultados: Antes do mascaramento, as cobaias nos grupos controle e miopia de privação de forma eram hipermetrópicas e não diferiam significativamente (p>0,05). A refração hipermetrópica no grupo controle diminuiu gradualmente. Nas cobaias do grupo de miopia de privação de forma, a potência de refração mudou gradualmente de +2,14 ± 0,33 D para -7,22 ± 0,51 D e o comprimento axial mudou gradualmente de 5,92 ± 0,37 mm para 8,05 ± 0,34 mm desde antes até o final do mascaramento. Antes do mascaramento, nenhuma diferença significativa foi observada nos níveis de expressão de mRNA e proteína de colágeno I e periostina na esclera dos grupos controle e miopia de privação de forma (p>0,05). Os níveis relativos de expressão de colágeno I e proteína periostina e mRNA na esclera de cobaias no grupo de miopia de privação de forma em 2, 4 e 8 semanas, e após cobertura dos olhos por 4 semanas seguido de descoberta por 2 semanas, foram significativamente menores que aqueles no grupo controle (p<0,05). Os níveis de expressão de mRNA, colágeno I e proteína periostina foram positivamente correlacionados com os níveis de expressão de proteína na esclera das cobaias (proteína: r=0,936, p<0,05; mRNA: r=0,909, p<0,05). Conclusões: A periostina foi expressa na esclerótica míope de cobaias e as alterações na expressão de periostina e colágeno I foram altamente consistentes. A periostina e o colágeno I podem estar envolvidos na regulação do remodelamento escleral na miopia.
Descritores: Esclera
Miopia Degenerativa
-RNA Mensageiro
Colágeno
Modelos Animais de Doenças
Cobaias
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: biblio-950727
Autor: Urriola-Muñoz, Paulina; Lizama, Carlos; Lagos-Cabré, Raúl; Reyes, Juan G; Moreno, Ricardo D.
Título: Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation
Fonte: Biol. Res;47:1-12, 2014. ilus, graf.
Idioma: en.
Projeto: FONDECYT.
Resumo: BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in different tissues. ADAM17 and ADAM10 are two members of this family surface metalloproteases involved in germ cell apoptosis during the first wave of spermatogenesis in the rat, but they have other signaling functions in somatic tissues. RESULTS: In an attempt to further study these two enzymes, we describe the presence and localization in adult male rats. Results showed that both enzymes are detected in germ and Sertoli cells during all the stages of spermatogenesis. Interestingly their protein levels and cell surface localization in adult rats were stage-specific, suggesting activation of these enzymes at particular events of rat spermatogenesis. CONCLUSIONS: Therefore, these results show that ADAM10 and ADAM17 protein levels and subcellular (cell surface) localization are regulated during rat spermatogenesis.
Descritores: Espermatogênese/fisiologia
Espermatozoides/metabolismo
Proteínas ADAM/metabolismo
-Túbulos Seminíferos/química
Células de Sertoli/citologia
Células de Sertoli/metabolismo
Espermátides/citologia
Espermátides/metabolismo
Testículo/anatomia & histologia
RNA Mensageiro/análise
Imuno-Histoquímica
Diferenciação Celular/fisiologia
Ratos Sprague-Dawley
Apoptose/fisiologia
Receptor fas/análise
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Proteínas ADAM/análise
Proteína ADAM10
Proteína ADAM17
Limites: Animais
Masculino
Ratos
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1041392
Autor: Tanikawa, Aline Aki; Grotto, Rejane Maria Tommasini; Silva, Giovanni Faria; Ferrasi, Adriana Camargo; Sarnighausen, Valéria Cristina Rodrigues; Pardini, Maria Inês de Moura Campos.
Título: Platelet-derived growth factor A mRNA in platelets is associated with the degree of hepatic fibrosis in chronic hepatitis C
Fonte: Rev. Soc. Bras. Med. Trop;50(1):113-116, Jan.-Feb. 2017. tab.
Idioma: en.
Resumo: Abstract: INTRODUCTION: Transforming growth factor beta 1 (TGFB1) and platelet-derived growth factor (PDGF) are the main cytokines related to hepatic fibrogenesis. METHODS: RNA isolated from the platelets and hepatic tissue of 43 HCV carriers was used for quantitative polymerase chain reaction to determine TGFB1, PDGFA, and PDGFB RNA expression. RESULTS: The mRNA expression of PDGFA in platelets was significantly lower in the group with advanced fibrosis than in the group with early-stage fibrosis. TGFB1 was more frequently expressed in platelets than in hepatic tissue, which was different from PDGFB. CONCLUSIONS: A pathway mediated by overexpression of TGFB1 via PDGFA in megakaryocytes could be involved in the development of fibrosis.
Descritores: Fator de Crescimento Derivado de Plaquetas/análise
Hepatite C Crônica/sangue
Proteínas Proto-Oncogênicas c-sis/sangue
Fator de Crescimento Transformador beta1/sangue
Cirrose Hepática/sangue
-Índice de Gravidade de Doença
Plaquetas/química
RNA Mensageiro/análise
Reação em Cadeia da Polimerase
Hepatite C Crônica/complicações
Cirrose Hepática/virologia
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adulto
Responsável: BR1.1 - BIREME



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