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Monteiro, Hugo Pequeno
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Id: biblio-886187
Autor: Oliveira, Teresinha Regina Ribeiro de; Oliveira, Geraldo Ferreira de; Simões, Ricardo Santos; Feitosa, Suellen Maurim; Tikazawa, Eduardo Hiroshi; Monteiro, Hugo Pequeno; Fagundes, Djalma José; Taha, Murched Omar.
Título: The expression of endothelial and inducible nitric oxide synthase and apoptosis in intestinal ischemia and reperfusion injury under the action of ischemic preconditioning and pentoxifylline
Fonte: Acta cir. bras;32(11):935-948, Nov. 2017. graf.
Idioma: en.
Resumo: Abstract Purpose: To investigate the expression of nitric oxide synthase (NOS) and apoptosis associated with ischemic preconditioning (IPC) and pentoxifylline (PTX) in intestinal ischemia (I) and reperfusion (R) injury. Methods: Thirty male rats were assigned to 5 groups: (CG), no clamping of the superior mesenteric artery (90 minutes); (IR-SS) saline + ischemia (30 minutes) + reperfusion (60 minutes); (IR-PTX) PTX + ischemia (30 minutes) + reperfusion (60 minutes); (IPC-IR-SS) 5 minutes of ischemia + 5 minutes of reperfusion (IPC) + saline + I(30 minutes)+R(60 minutes); and (IPC-IR-PTX) IPC + PTX + I(30 minutes)+ R(60 minutes). Results: The application of IPC and PTX showed a significantly lower immunohistochemistry reaction for active caspase-3 (P<0.05) compared to IR+SS. The number of cells immunoreactive to BCL-2 was higher in the IR-PTX group (P>0.05). The NOS-2 expression (qRTPCR) in the IR-PTX group (P<0.05) was higher than the values for the IPC+IR-SS and IPC-IR-PTX groups. The NOS-3 expression was significantly upper in the IPC-IR-PTX group than in the CG (P<0.05), the IR-SS (P<0.05) and the IR-PTX (P<0.05) groups. Conclusions: The BCL-2 and active caspase-3 showed beneficial effects on PTX and IPC. The expression of NOS-2 and NOS-3 in the IPC and IPC-PTX groups showed no synergistic effect.
Descritores: Pentoxifilina/uso terapêutico
Apoptose/efeitos dos fármacos
Óxido Nítrico Sintase/metabolismo
Precondicionamento Isquêmico
Enteropatias/prevenção & controle
Intestinos/irrigação sanguínea
-Vasodilatadores/uso terapêutico
RNA Mensageiro/análise
Imuno-Histoquímica
Ratos Wistar
Apoptose/fisiologia
Modelos Animais de Doenças
Enteropatias/enzimologia
Intestinos/patologia
Limites: Seres Humanos
Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: lil-233880
Autor: Januário, Adriana; Pietro, Rosemeire C. L. Rodrigues; Rodrigues, Evandro L. L; França, Celso A. de; Silva, Celio L.
Título: Produçäo de interleucinas RNAm em camundongos BALB/c infectados por paracoccidioides brasiliensis, com análise dos resultados através de processamento de imagens / RNAm interleukins production in BALB/c mice infected by Paracoccidioides brasiliensis, with resulted analysis by of the images processing
Fonte: In: Schiabel, Homero; Slaets, Annie France Frère; Costa, Luciano da Fontoura; Baffa Filho, Oswaldo; Marques, Paulo Mazzoncini de Azevedo. Anais do III Fórum Nacional de Ciência e Tecnologia em Saúde. Säo Carlos, s.n, 1996. p.584-584, ilus.
Idioma: pt.
Conferência: Apresentado em: Fórum Nacional de Ciência e Tecnologia em Saúde, 3 e Congresso Brasileiro de Engenharia Biomédica, 15 e Congresso Brasileiro de Físicos em Medicina , 6 e Congresso Brasileiro de Informática em Saúde, 5 e Encontro Brasileiro de Proteçäo Radiológica, Campos do Jordäo, 13-17 out. 1996.
Resumo: A Paracoccidioidomicose é uma infecçäo fúngica progressiva causada pelo fungo dimórfico Paracoccidioides brasiliensis, caracterizada por uma reaçäo inflamatória crônica, granulomatosa e por uma depressäo da resposta imune mediada por célula. As interaçöes fungo-hospedeiroinduzem distúrbios imunerregulatórios que promovem alteraçöes na produçäo de citocinas. Iniciamos um estudo sobre a produçäo de RNAm para citocinas em camundongos BALB/c infectados com P. brasiliensis. No início da infecçäo, observamos produçäo equivalente para IL-2 e IL-10 RNAm, com predomínio de RNAm para IL-2 no pulmäo. Ao contrário, as células do fígado apresentaram maiores níveis para o RNAm de IL-10. Neste modelo , há uma mudança dinâmica dos níveis de RNAm para IL-2 e IL-10, sugerindo a presença de ambas populaçöes de células TC4 + . estes resultados e o progresso desta investigaçäo cintribuiräo para um melhor entendimento da imunopatologia da paracoccidioidomicose.
Descritores: Paracoccidioidomicose/epidemiologia
Processamento de Imagem Assistida por Computador
Interleucinas
Avaliação de Resultados (Cuidados de Saúde)/métodos
-Autorradiografia/estatística & dados numéricos
RNA Mensageiro
Hibridização Genética
Nylons
Limites: Animais
Camundongos
Responsável: BR1.1 - BIREME
BR1.1/3012.72


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Carvalho, Emília Campos de
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Id: lil-752510
Autor: Teixeira, Carla Regina de Souza; Pereira, Marta Cristiane Alves; Kusumota, Luciana; Gaioso, Vanessa Pirani; Mello, Carolina Lima de; Carvalho, Emília Campos de.
Título: Avaliação dos estudantes de enfermagem sobre a aprendizagem com a simulação clínica / Evaluación de los estudiantes de enfermería sobre el aprendizaje con la simulación clínica / Evaluation of nursing students about learning with clinical simulation
Fonte: Rev. bras. enferm;68(2):311-319, Mar-Apr/2015. tab.
Idioma: pt.
Projeto: Conselho Nacional de Desenvolvimento Científico e Tecnológico.
Resumo: RESUMO Objetivo: descrever as contribuições da simulação clínica para aprendizagem de atributos cognitivos e procedimentais, por meio do debriefing, na perspectiva dos estudantes de enfermagem. Método: estudo descritivo exploratório. Participaram 20 estudantes de Graduação em Enfermagem de uma universidade do interior paulista. Na coleta de dados, realizada na etapa do debriefing, foi registrada a percepção do aluno sobre a simulação, aspectos positivos e o que poderia ser feito de forma diferente. Os relatos foram agrupados em categorias temáticas centrais, segundo referencial de análise de conteúdo de Bardin (2011), analisadas por meio de estatística descritiva. Resultados: identificada valorização da aprendizagem ativa, crítica e reflexiva (47,5%) em decorrência da aproximação à realidade assistencial (20,3%), manifestação dos sentimentos vivenciados durante a simulação (16,9%) e composição do cenário (15,3%). Conclusão: a simulação clínica seguida do debriefing favorece a compreensão da relação entre ação e resultados alcançados na aprendizagem. .

RESUMEN Objetivo: describir las contribuciones de simulación clínica para aprender atributos cognitivos y de procedimiento, a través de debriefing, desde la perspectiva de los estudiantes de enfermería. Método: estudio exploratorio descriptivo. 20 estudiantes participaron en el Pregrado en Enfermería de una universidad de São Paulo. Durante la recolección de datos, que se aplicó durante el debriefing, fue grabado en la percepción de los estudiantes de la simulación, los aspectos positivos y lo que podría hacerse de otra manera. Los informes de los estudiantes se agrupan de acuerdo a los temas centrales, según el referencial de análisis de contenido de Bardin (2011) y analizados mediante estadística descriptiva. Resultados: identificado la mejora de aprendizaje activo, crítico y reflexivo (47,5%) debido a la aproximación a la realidad en la atención de enfermería (20,3%), un resultado de la composición del escenario (16,9%), lo que favorece el desarrollo de sentimientos experimentados durante la simulación (15,3%). Conclusión: la simulación clínica seguida de debriefing favorece la comprensión de la relación entre la acción y los resultados obtenidos en el aprendizaje. .

ABSTRACT Objective: to describe the contributions of clinical simulation for learning cognitive and procedural attributes through debriefi ng, from the perspective of nursing students. Method: descriptive exploratory study. Twenty nursing undergraduate students from a university in the interior of the state of São Paulo participated in this study. Data collection was performed at the debriefi ng stage. Student’s perceptions about the simulation, positive aspects and what they could have done differently were registered. The students’ statements were grouped according to the central themes and the framework of Bardin’s content analysis (2011) and were analyzed using descriptive statistics. Results: enhancement of active, critical and refl ective learning (47.5%) was identifi ed due to the closeness to reality in nursing care (20.3%), manifestation of feelings experienced during the simulation (15.3%) and composition of the scenario (15.3%). Conclusion: the clinical simulation followed by debriefi ng promotes the understanding of the link between action and achievements in learning. .
Descritores: Proteínas de Arabidopsis/metabolismo
Arabidopsis/crescimento & desenvolvimento
Arabidopsis/imunologia
Imunidade Inata/imunologia
Fragmentos de Peptídeos/imunologia
Imunidade Vegetal/imunologia
Receptores de Reconhecimento de Padrão/imunologia
-Sequência de Aminoácidos
Arabidopsis/genética
Western Blotting
Regulação da Expressão Gênica de Plantas
Dados de Sequência Molecular
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/imunologia
Raízes de Plantas/metabolismo
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Receptores de Reconhecimento de Padrão/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência de Aminoácidos
Transdução de Sinais
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-618186
Autor: Wei, C. B; Chen, J.
Título: A novel lipocalin homologue from the venom gland of Deinagkistrodon acutus similar to mammalian lipocalins
Fonte: J. venom. anim. toxins incl. trop. dis;18(1):16-23, 2012. ilus.
Idioma: en.
Projeto: Natural Scientific Foundation of Anhui Province Education Commission.
Resumo: Lipocalins are involved in a variety of functions including retinol transport, cryptic coloration, olfaction, pheromone transport, prostaglandin synthesis, regulation of the immune response and cell homeostatic mediation. A full-length cDNA clone (named d-lipo), isolated from the venom gland cDNA library of Deinagkistrodon acutus, contained an insert of 664 bp including an open reading frame that encodes a lipocalin homologue of 177 amino acids. Comparison of d-lipo and other related proteins revealed an overall amino acid identity of less than 21.5 percent. Primary structures of d-lipo carried three structurally conserved regions (SCR) showing homologies to those of lipocalins. The first conserved Cys residue - the essential amino acid residue for the catalytic activity and unique to lipocalin-type prostaglandin D synthase (L-PGDS) in the lipocalin protein family - was identified in d-lipo at amino acid position 58. Phylogenetic tree analysis showed that d-lipo was in-between the large L-PGDS cluster and the small von Ebner's-gland proteins (VEGP) cluster. Moreover, d-lipo gene presented a high-level expression in the venom gland and a low-level expression in the brain and its expression was significantly increased under pathological conditions, suggesting a possible relationship between d-lipo mRNA expression and the venom gland inflammatory disease. This is also the first report of a lipocalin homologous gene identified in the venom gland of a snake.(AU)
Descritores: Venenos de Serpentes
Homologia de Sequência de Aminoácidos
Lipocalinas/química
-RNA Mensageiro
Biblioteca Gênica
Análise de Sequência de DNA
Limites: Animais
Responsável: BR33.1 - Divisão Técnica de Biblioteca e Documentação


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Id: biblio-1021049
Autor: Lei, Chao; Hao, Ruijuan; Zheng, Zhe; Deng, Yuewen; Wang, Qingheng; Li, Junhui.
Título: Molecular cloning and characterisation of scavenger receptor class B in pearl oyster Pinctada fuctada martensii
Fonte: Electron. j. biotechnol;30:12-17, nov. 2017. tab, graf.
Idioma: en.
Projeto: Graduate Education Innovation Program of Guangdong Ocean University; . Administration of Ocean and Fisheries of Guangdong Province; . Innovation Strong School Project of Guangdong Province.
Resumo: Background: Molluscs can accumulate carotenoids in their body tissues by predominantly feeding on aquatic plant sources. Carotenoid transport and absorption are determined by the regulation of various proteins such as Scavenger receptor class B(SR-BI). We report the identification and characterisation of pearl oyster Pinctada fuctada martensii SR-BI (PmSR-BI). The correlation between total carotenoid content (TCC) and gene expression was also estimated. Results: The full-length cDNA of PmSR-BI was 1828 bp, including an open-reading frame encoding of 1518 bp with a pI value of 5.83. PmSR-BI protein contains a hydrophobic CD36 domain and four centrally clustered cysteine residues for the arrangement of disulphide bridges. The deduced amino acid sequence had an identity of 30% to 60% with the SR-B of other organisms. Reverse transcription polymerase chain reaction analysis showed that mRNA transcripts were expressed in multiple tissues of adult pearl oyster. A higher expression of PmSR-BI gene was observed in the hepatopancreas than in the adductor muscle, gill and mantle. The TCC and gene expression of PmSR-BI were significantly correlated (P b 0.05), with a correlation coefficient of 0.978. Conclusions: The results suggested that PmSR-BI is involved in the absorption of carotenoids in the pearl oyster P. fuctada martensii.
Descritores: Carotenoides/metabolismo
Pinctada
Receptores Depuradores/genética
Receptores Depuradores/metabolismo
-Terpenos
Vitamina A/metabolismo
RNA Mensageiro/genética
Expressão Gênica
Clonagem Molecular
Análise de Sequência
Ácido Abscísico
DNA Complementar/genética
Interações Hidrofóbicas e Hidrofílicas
Reação em Cadeia da Polimerase em Tempo Real
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1017093
Autor: Alipour, Hamzeh; Raz, Abbasali; Zakeri, Sedigheh; Dinparast Djadid, Navid.
Título: Molecular characterization of matrix metalloproteinase-1 (MMP-1) in Lucilia sericata larvae for potential therapeutic applications
Fonte: Electron. j. biotechnol;29:47-56, sept. 2017. ilus, tab.
Idioma: en.
Projeto: Pasteur Institute of Iran; . Iranian National Science Foundation.
Resumo: Background: The salivary glands of Lucilia sericata are the first organs to express specific endopeptidase enzymes. These enzymes play a central role in wound healing, and they have potential to be used therapeutically. Methods: Rapid amplification of cDNA ends and rapid amplification of genomic ends were used to identify the coding sequence of MMP-1 from L. sericata. Different segments of MMP1 gene, namely the middle part, 3' end, and 5' end, were cloned, sequenced, and analyzed using bioinformatics tools to determine the distinct features of MMP-1 protein. Results: Assembling the different segments revealed that the complete mRNA sequence of MMP-1 is 1932 bp long. CDS is 1212 bp long and is responsible for the production of MMP-1 of 404 amino acid residues with a predicted molecular weight of 45.1 kDa. The middle part, 3' end, and 5' end sequences were 933, 503, and 496 bp. In addition, it was revealed that the MMP-1 genomic sequence includes three exons and two introns. Furthermore, the three-dimensional structure of L. sericata MMP-1 protein was evaluated, and its alignment defined that it has high similarity to chain A of human MMP-2 with 100% confidence, 72% coverage, and 38% identity according to the SWISS-MODEL modeling analysis. Conclusions: MMP-1 of L. sericata has a close relationship with its homologs in invertebrates and other insects. The present study significantly contributes to understanding the function, classification, and evolution of the characterized MMP-1 from L. sericata and provides basic required information for the development of an effective medical bioproduct.
Descritores: Glândulas Salivares/enzimologia
Metaloproteinase 1 da Matriz/genética
Dípteros/enzimologia
Dípteros/genética
-RNA Mensageiro/genética
Reação em Cadeia da Polimerase
Análise de Sequência de RNA
DNA Complementar/genética
Biologia Computacional
Larva
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-1008840
Autor: Kang, Bo; Jiang, Dongmei; He, Hui; Ma, Rong; Yi, Zhixin; Chen, Ziyu.
Título: Characterization of OAZ1 and its potential functions in goose follicular development
Fonte: Electron. j. biotechnol;26:1-6, Mar. 2017. ilus, graf.
Idioma: en.
Projeto: National Natural Science Foundation of China; . Specialized Research Fund for the Doctoral Program of Higher Education.
Resumo: Background: Ornithine decarboxylase antizyme 1 (OAZ1) is an important regulator of polyamine synthesis and uptake. Our previous studies indicated that high OAZ1 expression in the ovaries of laying geese is responsible for poor egg production. In the present study, the molecular characterization of goose OAZ1 gene was analyzed, as well as the expression profile in various follicular tissues. Results: An 873-bp cDNA sequence of the OAZ1 gene (Accession No. KC845302) with a +1 frameshift site (+175T) was obtained. The sequence consisted of a 652-bp two overlapping open reading frames (a putative protein with 216 amino acids). The OAZ domain, OAZ signature and OAZ super family domain were prominent conserved regions among species. As the follicle size increased, OAZ1 abundance showed an increasing trend during follicular development, while it decreased during follicular regression. The level of OAZ1 mRNA expression was the lowest in the fifth largest preovulatory follicle, and was 0.65-fold compared to the small white follicle (P b 0.05). OAZ1 mRNA expression in the largest preovulatory and postovulatory follicle was 2.11- and 2.49-fold compared to the small white follicle, respectively (P b 0.05). Conclusions: The goose OAZ1 structure confirms that OAZ1 plays an important role in ornithine decarboxylase-mediated regulation of polyamine homeostasis. Our findings provide an evidence for a potential function of OAZ1 in follicular development, ovulation and regression.
Descritores: Proteínas/genética
Proteínas/metabolismo
Gansos/metabolismo
Folículo Ovariano/metabolismo
-Ornitina Descarboxilase/metabolismo
Poliaminas/metabolismo
RNA Mensageiro
Clonagem Molecular
Análise de Sequência
DNA Complementar
Reação em Cadeia da Polimerase em Tempo Real
Folículo Ovariano/crescimento & desenvolvimento
Limites: Animais
Feminino
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-889146
Autor: Ono, Ekaterina Alexandrovna Durymanova; Taniwaki, Sueli Akemi; Brandão, Paulo.
Título: Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA
Fonte: Braz. j. microbiol;48(3):566-569, July-Sept. 2017. tab, graf.
Idioma: en.
Projeto: FAPESP; . National Counsel of Technological and Scientific Development.
Resumo: Abstract The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3):879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1 log decrease in virus titter and 5.16-fold reduction in P mRNA, 24 h post-inoculation when compared to non-treated cells. In vivo, siRNA 360 was able to induce partial protection, but with no significant difference when compared to non-treated mice. These results indicate that, despite the need for improvement for in vivo applications, P mRNA might be a target for an RNAi-based treatment for rabies.
Descritores: Fosfoproteínas/genética
Raiva/veterinária
Vírus da Raiva/genética
Proteínas Virais/genética
Quirópteros/virologia
RNA Interferente Pequeno/genética
Interferência de RNA
-Fosfoproteínas/metabolismo
Raiva/virologia
Vírus da Raiva/fisiologia
Proteínas Virais/metabolismo
Replicação Viral
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/metabolismo
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: biblio-889106
Autor: Jasso-Villagomez, EI; Garcia-Lorenzana, M; Almanza-Perez, JC; Fortis-Barrera, MA; Blancas-Flores, G; Roman-Ramos, R; Prado-Barragan, LA; Alarcon-Aguilar, FJ.
Título: Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARgamma expression
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;51(6):e7238, 2018. tab, graf.
Idioma: en.
Projeto: CONACyT; . PROMEP-SEP.
Resumo: Ulomoides dermestoides is a beetle traditionally consumed to treat diabetes. In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Normal mice and alloxan-induced diabetic mice were administered fractions of chitin, protein or fat, and the acute hypoglycemic effect was evaluated. A subacute study involving daily administration of these fractions to diabetic mice was also performed over 30 days, after which the liver and pancreas were processed by conventional histological techniques and stained with hematoxylin and eosin to evaluate morphological changes. The most active fraction, the fat fraction, was analyzed by gas chromatography-mass spectrometry (GC-MS), and PPARγ and GLUT4 mRNA expressions were determined in 3T3-L1 adipocytes. The protein and fat fractions exhibited hypoglycemic effects in the acute as well as in the 30-day study. Only the fat fraction led to elevated insulin levels and reduced glycemia, as well as lower intake of water and food. In the liver, we observed recovery of close hepatic cords in the central lobule vein following treatment with the fat fraction, while in the pancreas there was an increased density and percentage of islets and number of cells per islet, suggesting cellular regeneration. The GC-MS analysis of fat revealed three fatty acids as the major components. Finally, increased expression of PPARγ and GLUT4 was observed in 3T3-L1 adipocytes, indicating an antidiabetic effect.
Descritores: Pâncreas/efeitos dos fármacos
Extratos de Tecidos/uso terapêutico
Coleópteros/química
Corpo Adiposo/química
Hipoglicemiantes/uso terapêutico
Fígado/efeitos dos fármacos
-Pâncreas/metabolismo
Pâncreas/patologia
Extratos de Tecidos/isolamento & purificação
RNA Mensageiro/efeitos dos fármacos
RNA Mensageiro/metabolismo
Regulação da Expressão Gênica
PPAR gama/efeitos dos fármacos
PPAR gama/metabolismo
Diabetes Mellitus Experimental/metabolismo
Diabetes Mellitus Experimental/patologia
Diabetes Mellitus Experimental/tratamento farmacológico
Transportador de Glucose Tipo 4/efeitos dos fármacos
Transportador de Glucose Tipo 4/metabolismo
Hipoglicemiantes/isolamento & purificação
Fígado/metabolismo
Fígado/patologia
Cromatografia Gasosa-Espectrometria de Massas
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-889067
Autor: Ma, Huiwen; Wang, Ping; Jin, Dan; Jia, Ting; Mao, Hong; Zhang, Jiandi; Zhao, Shi.
Título: The hepatic ectonucleotide pyrophosphatase/phosphodiesterase 1 gene mRNA abundance is reduced by insulin and induced by dexamethasone
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;51(4):e6980, 2018. graf.
Idioma: en.
Projeto: National Natural Science Foundation.
Resumo: Hormones regulate hepatic gene expressions to maintain metabolic homeostasis. Ectonucleotide pyrophosphatase/phosphodiesterase 1 has been thought to interfere with insulin signaling. To determine its potential role in the regulation of metabolism, we analyzed its gene (Enpp1) expression in the liver of rats experiencing fasting and refeeding cycles, and in primary rat hepatocytes and human hepatoma HepG2 cells treated with insulin and dexamethasone using northern blot and real-time PCR techniques. Hepatic Enpp1 expression was induced by fasting and reduced by refeeding in the rat liver. In primary rat hepatocytes and HepG2 hepatoma cells, insulin reduced Enpp1 mRNA abundance, whereas dexamethasone induced it. Dexamethasone disrupted the insulin-reduced Enpp1 expression in primary hepatocytes. This is in contrast to the responses of the expression of the cytosolic form of phosphoenolpyruvate carboxykinase gene to the same hormones, where insulin reduced it significantly in the process. In addition, the dexamethasone-induced Enpp1 gene expression was attenuated in the presence of 8-Br-cAMP. In conclusion, we demonstrated for the first time that hepatic Enpp1 is regulated in the cycle of fasting and refeeding, a process that might be attributed to insulin-reduced Enpp1 expression. This insulin-reduced Enpp1 expression might play a role in the development of complications in diabetic patients.
Descritores: Pirofosfatases/genética
RNA Mensageiro/efeitos dos fármacos
Dexametasona/farmacologia
Diester Fosfórico Hidrolases/genética
Glucocorticoides/farmacologia
Hipoglicemiantes/farmacologia
Insulina/farmacologia
Fígado/enzimologia
-Pirofosfatases/biossíntese
Pirofosfatases/efeitos dos fármacos
Resistência à Insulina
RNA Mensageiro/metabolismo
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Indução Enzimática/efeitos dos fármacos
Jejum/metabolismo
Ratos Sprague-Dawley
Diester Fosfórico Hidrolases/biossíntese
Diester Fosfórico Hidrolases/efeitos dos fármacos
Células Hep G2
Reação em Cadeia da Polimerase em Tempo Real
Limites: Seres Humanos
Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde