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Id: biblio-955110
Autor: Costa, Jimena Ferreira da; Ferrarini, Mariana Galvão; Nardelli, Sheila Cristina; Goldenberg, Samuel; Ávila, Andréa Rodrigues; Holetz, Fabíola Barbieri.
Título: Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
Fonte: Mem. Inst. Oswaldo Cruz;113(6):e170531, 2018. graf.
Idioma: en.
Resumo: BACKGROUND Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. OBJECTIVES This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. METHODS We used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. FINDINGS TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. MAIN CONCLUSIONS TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism.
Descritores: RNA/sangue
RNA Mensageiro/análise
Metaciclina/uso terapêutico
-RNA Nuclear Pequeno
Limites: Humanos
Responsável: BR1.1 - BIREME


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Id: lil-526208
Autor: Pavía, Paula Ximena; Cuervo, Claudia L; Gil, Juliana; Romero, Ibeth; Morales, Liliana; Díez, Hugo; Quintero, Claudia; del Portillo, Patricia; Adolfo Vallejo, Gustavo; Florez, Astrid C; Montilla, Marleny; Mercado, Marcela; Vacca, Miguel; Nicholls, Rubén Santiago; López, Manuel C; Puerta, Concepción J.
Título: Caracterización molecular de los genes histona H2A y ARNsno-Cl de Trypanosoma rangeli: aplicación en pruebas diagnósticas / Molecular characterization of histone H2A and snoRNA-Cl genes of Trypanosoma rangeli: application in diagnostic tests
Fonte: Infectio;13(1):43-57, 2009. ilus, tab.
Idioma: es.
Resumo: La aplicación de la reacción en cadena de la polimerasa (PCR) para detectar e identificar Trypanosoma rangeli y Trypanosoma rangeli presenta a menudo dificultades de interpretación. Así, algunas pruebas generan la amplificación de bandas similares provenientes de uno de los dos parásitos, fragmentos polimórficos de un mismo parásito, o la prevalencia en la detección de T. cruzi en infecciones mixtas. En este estudio se presentan y analizan los trabajos de investigación básica realizados con el objeto de diseñar y estandarizar pruebas de PCR específicas de cada parásito. Los iniciadores TcH2AF/R se diseñaron sobre la base de la región diferencial observada entre las unidades génicas que contienen los genes h2a en estos tripanosomas. Esta pareja de iniciadores amplifican un fragmento de 234 pb específico para T. cruzi (cepas I y II). Los iniciadores TrF/R2 anillan en las regiones intergénicas del fragmento génico de 801 pb codificante para seis transcritos que forman la agrupación ARNsno-Cl en T. rangeli. Estos iniciadores amplifican un fragmento de 620 pb exclusivo de las cepas KP1(-) y KP1(+) de este parásito. La aplicación de estas PCR en vectores infectados y en pacientes con enfermedad de Chagas muestra que ambas pruebas constituyen herramientas útiles para el diagnóstico y la identificación diferencial de estos tripanosomátidos.

The application of polymerase chain reaction (PCR) to detect Trypanosoma rangeli and Trypanosoma rangeli often presents interpretation challenges. For example, some tests yield the amplification of similar bands from either parasite, polymorphic fragments of the same parasite, or present deviation towards T. cruzi in mixed infections. In this study, the basic researching needed for designing and standardizating specific PCR tests for each parasite species PCR are shown and analyzed. The TcH2AF/R primers were designed on the basis of the differential gene region observed between the histone h2a genic units of these parasites. These primers amplify a specific 234 bp fragment in T. cruzi (T. cruzi I and II strains). The TrF/R2 primers anneal to the intergenic regions of an 801 bp gene fragment encoding for six transcripts that conform the snoRNA-Cl cluster in T. rangeli. These primers amplify a fragment of 620 bp exclusively in KP1(-) and KP1(+) strains of the parasite. The application of these PCR tests in infected vectors and in chagasic patients show that both tests constitute useful tools for the diagnosis and differential identification of these Trypanosomatids. Key words: histone, RNA small nucleolar (snoRNA), polymerase chain reaction (PCR), Trypanosoma.
Descritores: Testes Diagnósticos de Rotina
Histonas
Reação em Cadeia da Polimerase
RNA Nuclear Pequeno
Trypanosoma
-Colômbia
Tipo de Publ: Revisão
Responsável: CO42.1 - Biblioteca Nacional de Salud José Celestino Mutis


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Id: lil-440625
Autor: Ambrósio, D. L; Silva, M. T. A; Cicarelli, R. M. B.
Título: Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
Fonte: Mem. Inst. Oswaldo Cruz;102(1):97-106, Feb. 2007. tab, ilus.
Idioma: en.
Projeto: Financiamento de Projetos de Extensão Universitária; . Fundação de Amparo à Pesquisa do Estado de São Paulo; . Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Programa de Apoio ao Desenvolvimento Científico.
Resumo: Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in splicing, catalyzed by spliceosomes, large RNA-protein complexes composed of various snRNPs. Even though they are well defined in mammals, snRNPs are still not totally characterized in certain trypanosomatids as Trypanosoma cruzi. For this reason we subjected snRNAs (U2, U4, U5, and U6) from T. cruzi epimastigotes to molecular characterization by polymerase chain reaction (PCR) and reverse transcription-PCR. These amplified sequences were cloned, sequenced, and compared with those other of trypanosomatids. Among these snRNAs, U5 was less conserved and U6 the most conserved. Their respective secondary structures were predicted and compared with known T. brucei structures. In addition, the copy number of each snRNA in the T. cruzi genome was characterized by Southern blotting.
Descritores: Genoma de Protozoário/genética
RNA Nuclear Pequeno/genética
Trypanosoma cruzi/genética
-Southern Blotting
Clonagem Molecular
Reação em Cadeia da Polimerase/métodos
Processamento de RNA
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-430953
Autor: Puerta, Concepción J.
Título: Identificación de una agrupación génica que codifica para ARN pequeños de nucleólo en Trypanosoma Rangeli / Identification of a Genetic Cluster Codingfor Six Small Nucleolar RNA in Trypanosoma rangeli
Fonte: Infectio;9(4):171-179, dic. 2005. ilus, tab.
Idioma: es.
Resumo: Objetivo. Realizar un análisis in silico de un fragmento de 801 pb de Trypanosoma rangeli, previamente reportado como codificante para el ARN pequeño de nucléolo C1, perteneciente a la familia de los C/D snoARN. Materiales y métodos. El tamizaje de las secuencias se realizó en las bases de datos de los diferentes proyectos de genomas de los parásitos usando el programa BLASTN. Las alineaciones de las secuencias se hicieron con los programas L-ALIGN y Clustal W. Resultados. La secuencia C1 constituye una agrupación génica que codifica para seis ARN pequeños de nucléolo, tres pertenecientes a la familia C/D snoARN y tres a la famila H/ACA snoARN. Conclusión. Los ARN pequeños de nucléolo de Trypanosomatidae presentan una organización genómica similar y elevados porcentajes de identidad en las secuencias consenso, lo cual unido a las funciones de estos ARN en el procesamiento y modificación posteriores a la transcripción del ARN ribosómico y, también, en el proceso de trans splicing, hace de estas moléculas un blanco atractivo para el control de estos parásitos
Descritores: RNA Nuclear Pequeno/genética
Análise de Sequência de DNA
Trypanosoma/genética
Responsável: CO42.1 - Biblioteca Nacional de Salud José Celestino Mutis


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Id: lil-225265
Autor: Flores, Georgina; Martínez, Filiberto; Reyes, Pedro A; Cortés, J. Jesús.
Título: Molecular analysis of snRNAs and scRNAs using autoantibodies from patients with systemic lupus erythematosus
Fonte: Arch. med. res;28(4):571-5, dec. 1997. ilus, tab.
Idioma: en.
Resumo: Immunoprecipitation analysis of total HeLa cells RNA extract byprotein A-Sheparose purified autoantibodies and pCp 32P-3' end labeling RNAs revealed that U1, U2, U4 and U5 snRNAs are related with anti-Sm or U1nRNP autoantibodies, while the hY1, hY3, hY4 and hY5 scRNAs were related to anti-SSA/Ro autoantibodies present in sera of patient with Systemic Lupus Erythematosus. The authors detected molecular snRNAs and scRNAs specificities by autoantibodies in 71 sera, the molecular RNA specificity for anti-Sm (U1, U2, U4 and U5 snRNAs) was present in 39 percent; anti-SSA/Ro sera reacted against scRNAs (hY1, hY3, hY4 and hY5) in 36 percent, then anti-U1nRNP sera recognized U1 snRNA in 13 percent of sera and anti-rRNP related with rRNA were recognized in 8 percent. Twenty-nine SLE sera were RNA negative. A molecular characterization of the autoantibodies in sera from SLE patients may be a useful tool for clinical and laboratory diagnosis of SLE, and the use of autoantibodies es molecular probes allows to continue exploring some basic mechanism of gene expression
Descritores: RNA Nuclear Pequeno/análise
RNA Nuclear Pequeno/imunologia
Autoanticorpos/sangue
Autoanticorpos/imunologia
Células HeLa
Lúpus Eritematoso Sistêmico/imunologia
Lúpus Eritematoso Sistêmico/sangue
Testes de Precipitina
RNA/análise
RNA/imunologia
Limites: Humanos
Responsável: MX1.1 - CENIDSP - Centro de Información para Decisiones en Salud Pública


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Id: lil-176756
Autor: Luciano, Edgar; Arroyo, Gerardo; Candelas, Teresa; Candelas, Graciela C.
Título: Prelude activities in the synthesis of tissue-specific secretory protein products
Fonte: P. R. health sci. j;11(2):73-6, ago. 1992.
Idioma: en.
Resumo: In studying the process of protein synthesis of a silk-producing organism we have found that several macromolecules must be synthesized in order for the process to occur. Through time course studies, we have found that small RNAs may play a paramount role in directing the finely orchestrated process. Alanine tRNA, U1 snRNA, and 5S RNA have been identified through Northern blotting as molecules timely and tissue-specific synthesized and upgraded as a prelude activity for the silk being produced
Descritores: Fibroínas/biossíntese
Aranhas/metabolismo
-Northern Blotting
Eletroforese
RNA Nuclear Pequeno/isolamento & purificação
Limites: Animais
Feminino
Responsável: BR1.1 - BIREME



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