Base de dados : LILACS
Pesquisa : D13.444.735.686 [Categoria DeCS]
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Id: lil-623598
Autor: Mello, Maricilda P. de; Gomes, Henrique B. M; Guevara, Palmira; Ramirez, José L; Silveira, José Franco da.
Título: Trypanosoma cruzi: cloning of the 26 S Ribosomal gene and its associated spacer
Fonte: Mem. Inst. Oswaldo Cruz;83(supl.1):334-336, Nov. 1988.
Idioma: en.
Conferência: Apresentado em: Annual Meeting on Basic Research in Chagas's disease, 15, Apresentado em: Meeting of the Brazilian Society of Protozoology4, Caxambu, 7-10 Nov. 1988.
Descritores: RNA Ribossômico/genética
Clonagem Molecular
Responsável: BR1.1 - BIREME


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Id: lil-796906
Autor: Bardella, Vanessa Bellini; Pita, Sebastián; Vanzela, André Luis Laforga; Galvão, Cleber; Panzera, Francisco.
Título: Heterochromatin base pair composition and diversification in holocentric chromosomes of kissing bugs (Hemiptera, Reduviidae)
Fonte: Mem. Inst. Oswaldo Cruz;111(10):614-624, Oct. 2016. tab, graf.
Idioma: en.
Projeto: Udelar.
Resumo: The subfamily Triatominae (Hemiptera, Reduviidae) includes 150 species of blood-sucking insects, vectors of Chagas disease or American trypanosomiasis. Karyotypic information reveals a striking stability in the number of autosomes. However, this group shows substantial variability in genome size, the amount and distribution of C-heterochromatin, and the chromosome positions of 45S rDNA clusters. Here, we analysed the karyotypes of 41 species from six different genera with C-fluorescence banding in order to evaluate the base-pair richness of heterochromatic regions. Our results show a high heterogeneity in the fluorescent staining of the heterochromatin in both autosomes and sex chromosomes, never reported before within an insect subfamily with holocentric chromosomes. This technique allows a clear discrimination of the heterochromatic regions classified as similar by C-banding, constituting a new chromosome marker with taxonomic and evolutionary significance. The diverse fluorescent patterns are likely due to the amplification of different repeated sequences, reflecting an unusual dynamic rearrangement in the genomes of this subfamily. Further, we discuss the evolution of these repeated sequences in both autosomes and sex chromosomes in species of Triatominae.
Descritores: Cromossomos de Insetos/genética
Heterocromatina/genética
Insetos Vetores/genética
Triatominae/genética
-Evolução Biológica
Doença de Chagas/transmissão
DNA Ribossômico/genética
Cariotipagem
RNA Ribossômico/genética
Triatominae/classificação
Limites: Animais
Responsável: BR1.1 - BIREME


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Id: lil-755814
Autor: Santos, Bruna Silveira Lamanes dos; Gomes, Arthur Filipe Sousa; Franciscon, Emanuele Giuliane; Oliveira, Jean Maikon de; Baffi, Milla Alves.
Título: Thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production
Fonte: Braz. j. microbiol;46(3):903-910, July-Sept. 2015. tab, ilus.
Idioma: en.
Resumo:

Nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at Brazilian Cerrado and identified up to species level on the basis of the gene sequencing of 5.8S-ITS and 26S ribosomal DNA regions. Four species were identified: Kluyveromyces marxianus, Aspergillus niger, Aspergillus sydowii and Aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. Among them, three strains were selected as good producers of hemicellulolitic enzymes: A. niger (SBCM3), A. sydowii (SBCM7) and A. fumigatus (SBC4). The best β-xylosidase producer was A. niger SBCM3 strain. This crude enzyme presented optimal activity at pH 3.5 and 55 °C (141 U/g). For β-glucosidase and xylanase the best producer was A. fumigatus SBC4 strain, whose enzymes presented maximum activity at 60 °C and pH 3.5 (54 U/g) and 4.0 (573 U/g), respectively. All these crude enzymes presented stability around pH 3.0–8.0 and up to 60 °C, which can be very useful in industrial processes that work at high temperatures and low pHs. These enzymes also exhibited moderate tolerance to ethanol and the sugars glucose and xylose. These similar characteristics among these fungal crude enzymes suggest that they can be used synergistically in cocktails in future studies of biomass conversion with potential application in several biotechnological sectors.

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Descritores: Aspergillus fumigatus/enzimologia
Aspergillus niger/enzimologia
Celulose/metabolismo
/metabolismo
ENDO-1,ABBREVIATIONS AS TOPIC-BETA XYLANASES/metabolismo
Kluyveromyces/enzimologia
Saccharum/microbiologia
Xilosidases/metabolismo
beta-Glucosidase/metabolismo
-Aspergillus fumigatus/isolamento & purificação
Aspergillus fumigatus/metabolismo
Aspergillus niger/isolamento & purificação
Aspergillus niger/metabolismo
Sequência de Bases
Biomassa
Brasil
DNA Fúngico/genética
DNA Intergênico/genética
Fermentação
Kluyveromyces/isolamento & purificação
Kluyveromyces/metabolismo
Lignina/metabolismo
Tipagem Molecular
Técnicas de Tipagem Micológica
RNA Ribossômico/genética
Análise de Sequência de DNA
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-755801
Autor: Merín, María Gabriela; Martín, María Carolina; Rantsiou, Kalliopi; Cocolin, Luca; de Ambrosini, Vilma Inés Morata.
Título: Characterization of pectinase activity for enology from yeasts occurring in Argentine Bonarda grape
Fonte: Braz. j. microbiol;46(3):815-823, July-Sept. 2015. tab, ilus.
Idioma: en.
Resumo:

Pectinolytic enzymes are greatly important in winemaking due to their ability to degrade pectic polymers from grape, contributing to enhance process efficiency and wine quality. This study aimed to analyze the occurrence of pectinolytic yeasts during spontaneous fermentation of Argentine Bonarda grape, to select yeasts that produce extracellular pectinases and to characterize their pectinolytic activity under wine-like conditions. Isolated yeasts were grouped using PCR-DGGE and identified by partial sequencing of 26S rRNA gene. Isolates comprised 7 genera, with Aureobasidium pullulans as the most predominant pectinolytic species, followed by Rhodotorula dairenensis and Cryptococcus saitoi. No pectinolytic activity was detected among ascomycetous yeasts isolated on grapes and during fermentation, suggesting a low occurrence of pectinolytic yeast species in wine fermentation ecosystem. This is the first study reporting R. dairenensis and Cr. saitoi species with pectinolytic activity. R. dairenensis GM-15 produced pectinases that proved to be highly active at grape pH, at 12 °C, and under ethanol and SO2 concentrations usually found in vinifications (pectinase activity around 1.1 U/mL). This strain also produced cellulase activity at 12 °C and pH 3.5, but did not produce β-glucosidase activity under these conditions. The strain showed encouraging enological properties for its potential use in low-temperature winemaking.

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Descritores: Ascomicetos/enzimologia
Cryptococcus/enzimologia
Poligalacturonase/metabolismo
Rhodotorula/enzimologia
Vitis/microbiologia
Vinho/microbiologia
-Argentina
Ascomicetos/isolamento & purificação
Cryptococcus/isolamento & purificação
Fermentação/fisiologia
Dados de Sequência Molecular
Tipagem Molecular
Técnicas de Tipagem Micológica
Reação em Cadeia da Polimerase
Pectinas/metabolismo
RNA Ribossômico/genética
Rhodotorula/isolamento & purificação
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-723096
Autor: Ponzzes-Gomes, Camila M.P.B.S. de; Mélo, Dângelly L.F.M. de; Santana, Caroline A.; Pereira, Giuliano E.; Mendonça, Michelle O.C.; Gomes, Fátima C.O.; Oliveira, Evelyn S.; Barbosa Jr, Antonio M.; Trindade, Rita C.; Rosa, Carlos A..
Título: Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley
Fonte: Braz. j. microbiol;45(2):411-416, Apr.-June 2014. ilus, graf.
Idioma: en.
Resumo: The aims of this work was to characterise indigenous Saccharomyces cerevisiae strains in the naturally fermented juice of grape varieties Cabernet Sauvignon, Grenache, Tempranillo, Sauvignon Blanc and Verdejo used in the São Francisco River Valley, northeastern Brazil. In this study, 155 S. cerevisiae and 60 non-Saccharomyces yeasts were isolated and identified using physiological tests and sequencing of the D1/D2 domains of the large subunit of the rRNA gene. Among the non-Saccharomyces species, Rhodotorula mucilaginosa was the most common species, followed by Pichia kudriavzevii, Candida parapsilosis, Meyerozyma guilliermondii, Wickerhamomyces anomalus, Kloeckera apis, P. manshurica, C. orthopsilosis and C. zemplinina. The population counts of these yeasts ranged among 1.0 to 19 x 10(5) cfu/mL. A total of 155 isolates of S. cerevisiae were compared by mitochondrial DNA restriction analysis, and five molecular mitochondrial DNA restriction profiles were detected. Indigenous strains of S. cerevisiae isolated from grapes of the São Francisco Valley can be further tested as potential starters for wine production.
Descritores: Biodiversidade
Vitis/microbiologia
Leveduras/classificação
Leveduras/isolamento & purificação
-Brasil
Contagem de Colônia Microbiana
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Técnicas de Tipagem Micológica
Polimorfismo de Fragmento de Restrição
RNA Ribossômico/genética
Análise de Sequência de DNA
Leveduras/genética
Tipo de Publ: Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-709467
Autor: Silva-Bedoya, L.M.; Ramírez-Castrillón, M.; Osorio-Cadavid, E..
Título: Yeast diversity associated to sediments and water from two Colombian artificial lakes
Fonte: Braz. j. microbiol;45(1):135-142, 2014. ilus, tab.
Idioma: en.
Resumo: In Colombia, knowledge of the yeast and yeast-like fungi community is limited because most studies have focused on species with clinical importance. Sediments and water represent important habitats for the study of yeast diversity, especially for yeast species with industrial, biotechnological, and bioremediation potential. The main purpose of this study was to identify and compare the diversity of yeast species associated with sediment and water samples from two artificial lakes in Universidad del Valle (Cali-Colombia). Yeast samplings were performed from fifteen sediment samples and ten water samples. Grouping of similar isolates was initially based on colony and cell morphology, which was then complemented by micro/mini satellite primed PCR banding pattern analysis by using GTG5 as single primer. A representative isolate for each group established was chosen for D1/D2 domain sequencing and identification. In general, the following yeast species were identified: Candida albicans, Candida diversa, Candida glabrata, Candida pseudolambica, Cryptococcus podzolicus, Cryptococcus rajasthanensis, Cryptococcus laurentii, Williopsis saturnus, Hanseniaspora thailandica, Hanseniaspora uvarum, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, Torulaspora delbrueckii, Torulaspora pretoriensis, Tricosporon jirovecii, Trichosporon laibachii and Yarrowia lypolitica. Two possible new species were also found, belonging to the Issatchenkia sp. and Bullera sp. genera. In conclusion, the lakes at the Universidad del Valle campus have significant differences in yeast diversity and species composition between them.
Descritores: Biodiversidade
Lagos/microbiologia
Leveduras/classificação
Leveduras/isolamento & purificação
-Colômbia
DNA Fúngico/química
DNA Fúngico/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Sedimentos Geológicos/microbiologia
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
RNA Ribossômico/genética
Análise de Sequência de DNA
Microbiologia da Água
Leveduras/genética
Responsável: BR1.1 - BIREME


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Id: lil-709457
Autor: Shetty, Prakasham Reddy; Buddana, Sudheer Kumar; Tatipamula, Vinay Bharadwaj; Naga, Yaswanth Varanasi Venkata; Ahmad, Jamal.
Título: Production of polypeptide antibiotic from Streptomyces parvulus and its antibacterial activity
Fonte: Braz. j. microbiol;45(1):303-312, 2014. ilus, tab.
Idioma: en.
Resumo: A highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of Visakhapatnam sea coast, Bay of Bengal. Over all ten strains are isolated from the collected soil sediments. Among the ten actinomycetes strains the broad spectrum strain RSPSN2 was selected for molecular characterization, antibiotic production and its purification. The nucleotide sequence of the 1 rRNA gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with Streptomyces parvulus 1044 strain, Streptomyces parvulus NBRC 13193 and Streptomyces parvulus BY-F. From the taxonomic features, the actinomycetes isolate RSPSN2 matches with Streptomyces parvulus in the morphological, physiological and biochemical characters. Thus, it was given the suggested name Streptomyces parvulus RSPSN2. The active metabolite was extracted using ethyl acetate (1:3, v/v) at pH 7.0. The separation of active ingredient and its purification was performed by using both thin layer chromatography (TLC) and column chromatography (CC) techniques. Spectrometric studies such as UV-visible, FTIR, and NMR and mass were performed. The antibacterial activity of pure compound was performed by cup plate method against some pathogenic bacteria including of streptomycin resistant bacteria like (Pseudomonas mirabilis. Pseudomonas putida and Bacillus cereus). In conclusion, the collected data emphasized the fact that a polypeptide antibiotic (Actinomycin D) was produced by Streptomyces parvulus RSPSN2.
Descritores: Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Peptídeos/isolamento & purificação
Peptídeos/farmacologia
Streptomyces/metabolismo
-Antibacterianos/química
Técnicas de Tipagem Bacteriana
Cromatografia Líquida
Cromatografia em Camada Delgada
Análise por Conglomerados
DNA Bacteriano/química
DNA Bacteriano/genética
Sedimentos Geológicos/microbiologia
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Índia
Espectroscopia de Ressonância Magnética
Testes de Sensibilidade Microbiana
Filogenia
Peptídeos/química
RNA Ribossômico/genética
Análise de Sequência de DNA
Espectrofotometria Ultravioleta
Espectroscopia de Infravermelho com Transformada de Fourier
Streptomyces/isolamento & purificação
Responsável: BR1.1 - BIREME


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Id: lil-705816
Autor: Mourão, Maria Vitória Assumpção; Toledo Jr, Antonio; Gomes, Luciana Inácia; Freire, Verônica Vieira; Rabello, Ana.
Título: Parasite load and risk factors for poor outcome among children with visceral leishmaniasis. A cohort study in Belo Horizonte, Brazil, 2010-2011
Fonte: Mem. Inst. Oswaldo Cruz;109(2):147-153, abr. 2014. tab, graf.
Idioma: en.
Projeto: FAPEMIG; . CNPq.
Resumo: Clinical and laboratory risk factors for death from visceral leishmaniasis (VL) are relatively known, but quantitative real-time polymerase chain reaction (qPCR) might assess the role of parasite load in determining clinical outcome. The aim of this study was to identify risk factors, including parasite load in peripheral blood, for VL poor outcome among children. This prospective cohort study evaluated children aged ≤ 12 years old with VL diagnosis at three times: pre-treatment (T0), during treatment (T1) and post-treatment (T2). Forty-eight patients were included and 16 (33.3%) met the criteria for poor outcome. Age ≤ 12 months [relative risk (RR) 3.51; 95% confidence interval (CI) 1.89-6.52], tachydyspnoea (RR 3.46; 95% CI 2.19-5.47), bacterial infection (RR 3.08; 95% CI 1.27-7.48), liver enlargement (RR 3.00; 95% CI 1.44-6.23) and low serum albumin (RR 7.00; 95% CI 1.80-27.24) were identified as risk factors. qPCR was positive in all patients at T0 and the parasite DNA was undetectable in 76.1% of them at T1 and in 90.7% at T2. There was no statistical association between parasite load at T0 and poor outcome.
Descritores: Leishmania/isolamento & purificação
Leishmaniose Visceral/parasitologia
Avaliação de Resultados (Cuidados de Saúde)/normas
Carga Parasitária/estatística & dados numéricos
-Brasil/epidemiologia
Distribuição de Qui-Quadrado
DNA de Protozoário/isolamento & purificação
Dispneia/diagnóstico
Hepatomegalia
Leishmania/genética
Leishmaniose Visceral/diagnóstico
Leishmaniose Visceral/epidemiologia
Fígado/parasitologia
Estudos Prospectivos
Reação em Cadeia da Polimerase/normas
Fatores de Risco
RNA Ribossômico/sangue
Albumina Sérica
Estatísticas não Paramétricas
Baço/parasitologia
Limites: Criança
Pré-Escolar
Feminino
Seres Humanos
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-678294
Autor: Memorias do Instituto Oswaldo Cruz; Richards, Bethany; la Rua, Nicholas M de; Monroy, Carlota; Stevens, Lori; Dorn, Patricia L.
Título: Novel polymerase chain reaction-restriction fragment length polymorphism assay to determine internal transcribed spacer-2 group in the Chagas disease vector, Triatoma dimidiata (Latreille, 1811)
Fonte: Mem. Inst. Oswaldo Cruz;108(4):395-398, jun. 2013. tab, graf.
Idioma: en.
Projeto: NIH.
Resumo: Triatoma dimidiata is the most important Chagas disease insect vector in Central America as this species is primarily responsible for Trypanosoma cruzi transmission to humans, the protozoan parasite that causes Chagas disease. T. dimidiata sensu lato is a genetically diverse assemblage of taxa and effective vector control requires a clear understanding of the geographic distribution and epidemiological importance of its taxa. The nuclear ribosomal internal transcribed spacer 2 (ITS-2) is frequently used to infer the systematics of triatomines. However, oftentimes amplification and sequencing of ITS-2 fails, likely due to both the large polymerase chain reaction (PCR) product and polymerase slippage near the 5' end. To overcome these challenges we have designed new primers that amplify only the 3'-most 200 base pairs of ITS-2. This region distinguishes the ITS-2 group for 100% of known T. dimidiata haplotypes. Furthermore, we have developed a PCR-restriction fragment length polymorphism (RFLP) approach to determine the ITS-2 group, greatly reducing, but not eliminating, the number of amplified products that need to be sequenced. Although there are limitations with this new PCR-RFLP approach, its use will help with understanding the geographic distribution of T. dimidiata taxa and can facilitate other studies characterising the taxa, e.g. their ecology, evolution and epidemiological importance, thus improving vector control.
Descritores: DNA Espaçador Ribossômico/análise
Insetos Vetores/genética
RNA Ribossômico/análise
Triatoma/genética
-Doença de Chagas/transmissão
Guatemala
Amplificação de Genes/genética
Haplótipos
Insetos Vetores/classificação
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Triatoma/classificação
Limites: Animais
Tipo de Publ: Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Responsável: BR1.1 - BIREME


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Id: lil-674691
Autor: Assis, Dnieber Chagas; Resende, Deisy Vivian; Cabrine-Santos, Marlene; Correia, Dalmo; Oliveira-Silva, Márcia Benedita.
Título: Prevalence and genetic characterization of Cryptosporidium spp. and Cystoisospora belli in HIV-infected patients / Prevalência e caracterização genética de Cryptosporidium spp. e Cystoisospora belli em pacientes infectados pelo HIV
Fonte: Rev. Inst. Med. Trop. Säo Paulo;55(3):149-154, May-Jun/2013. graf.
Idioma: en.
Resumo: Cryptosporidium spp. and Cystoisospora belli are monoxenic protozoa that have been recognized as the causative agents of chronic diarrhea in immunocompromised individuals, especially HIV-infected subjects. The objective of this study was to evaluate the frequency of these intestinal protozoa in HIV-positive patients in the Triângulo Mineiro region of Brazil and to correlate the presence of these infections with clinical, epidemiological and laboratory data of the patients. Oocysts were detected in stool samples of 10 (16.9%) of the 59 patients studied, while Cryptosporidium spp. were present in 10.1% (6/59) and C. belli in 6.7% (4/59). The frequency of these parasites was higher among patients with diarrheic syndrome and CD4+ T lymphocyte counts < 200 cells/mm 3 , demonstrating the opportunistic characteristic of these infections. A significant association was observed between the lack of adherence to antiretroviral therapy and the presence of Cryptosporidium spp. and/or C. belli. Parasitism with Cryptosporidium spp. was more frequent in February and April, the months following the period of high rainfall. The same was not observed for C. belli. Genetic characterization of two isolates led to the identification of Cryptosporidium parvum, one of the main species associated with the zoonotic transmission of cryptosporidiosis.

Cryptosporidium spp. e Cystoisospora belli são protozoários monoxenos reconhecidos como agentes causadores de diarréia crônica em indivíduos imunocomprometidos, especialmente aqueles infectados pelo HIV. Os objetivos deste estudo foram o de avaliar a frequência destes protozoários em pacientes HIV - positivos na região do Triângulo Mineiro, Brasil, e correlacionar a presença destas infecções com dados clínicos, epidemiológicos e laboratoriais dos pacientes. Oocistos foram detectados em amostras fecais de 10 (16,9%) dos 59 pacientes estudados, sendo 10.1% (6/59) das amostras positivas para Cryptosporidium spp. e 6,7% (4/59) das amostras positivas para C. belli. A frequência destes parasitos foi maior entre pacientes com síndrome diarreica e contagem de linfócitos T CD4+ < 200 cells/mm 3 , o que demonstra o caráter oportunista destas infecções. Foi observada uma associação significativa entre a falta de aderência à terapia antiretroviral e a presença de Cryptosporidium spp. e/ou C. belli. Parasitismo por Cryptosporidium spp. foi mais frequente em fevereiro e abril, meses subsequentes ao período chuvoso. O mesmo não foi observado para C. belli. A caracterização genética de dois isolados levou à identificação de Cryptosporidium parvum, uma das principais espécies associadas com a transmissão zoonótica da criptosporidiose.
Descritores: Infecções Oportunistas Relacionadas com a AIDS/epidemiologia
Criptosporidiose/epidemiologia
Cryptosporidium/genética
-Infecções Oportunistas Relacionadas com a AIDS/diagnóstico
Infecções Oportunistas Relacionadas com a AIDS/parasitologia
Brasil/epidemiologia
Criptosporidiose/diagnóstico
Criptosporidiose/parasitologia
Cryptosporidium/classificação
Fezes/parasitologia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
RNA de Protozoário/análise
RNA Ribossômico/análise
Limites: Adolescente
Adulto
Feminino
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
Responsável: BR1.1 - BIREME



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