Base de dados : LILACS
Pesquisa : D13.444.735.686.670 [Categoria DeCS]
Referências encontradas : 43 [refinar]
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Id: biblio-951818
Autor: Boujelben, Ines; Gdoura, Radhouane; Hammami, Adnane.
Título: A broad-range PCR technique for the diagnosis of infective endocarditis
Fonte: Braz. j. microbiol;49(3):534-543, July-Sept. 2018. tab, graf.
Idioma: en.
Resumo: Abstract Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A broad-range PCR technique was selected and evaluated in terms of its efficiency in the diagnosis of endocarditis using 19 heart valves from patients undergoing cardiovascular surgeries at the Habib Bourguiba Hospital of Sfax, Tunisia, on the grounds of suspected IE. The results demonstrated the efficiency of this technique particularly in cases involving a limited number of bacteria since it helped to increase detection sensitivity. The technique proved to be efficient, particularly, in the bacteriological diagnosis of IE in contexts involving negative results from conventional culture methods and other contexts involving bacterial species that were not amenable to identification by phenotypic investigations. Indeed, the sequencing of the partial 16S ribosomal RNA gene revealed the presence of Bartonella henselae, Enterobacter sp., and Streptococcus pyogenes in three heart valves with the negative culture. It should be noted that the results obtained from the polymerase chain reaction-sequencing identification applied to the heart valve and the strain isolated from the same tissue were not consistent with the ones found by the conventional microbiological methods in the case of IE caused by Gemella morbillorum. In fact, the results from the molecular identification revealed the presence of Lactobacillus jensenii. Overall, the results have revealed that the proposed method is sensitive, reliable and might open promising opportunities for the early diagnosis of IE.
Descritores: Bactérias/isolamento & purificação
Reação em Cadeia da Polimerase/métodos
Endocardite/microbiologia
Endocardite Bacteriana/microbiologia
-Filogenia
Bactérias/classificação
Bactérias/genética
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Endocardite/diagnóstico
Endocardite Bacteriana/diagnóstico
Valvas Cardíacas/microbiologia
Meia-Idade
Limites: Seres Humanos
Masculino
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME


  2 / 43 LILACS  
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Id: biblio-951798
Autor: Rodrigues, Dalila Ribeiro; Silva, Aleksandro Ferreira da; Cavalcanti, Maria Idaline Pessoa; Escobar, Indra Elena Costa; Fraiz, Ana Carla Resende; Ribeiro, Paula Rose de Almeida; Ferreira Neto, Reginaldo Alves; Freitas, Ana Dolores Santiago de; Fernandes-Júnior, Paulo Ivan.
Título: Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest
Fonte: Braz. j. microbiol;49(3):503-512, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: Brazilian Council for Scientific and Technological Development.
Resumo: Abstract Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic genes. The bacteria showed a high variable metabolic profile. Bradyrhizobium (6), Rhizobium (3) and Paraburkholderia (1) were identified, differing from their geographic origin. The isolates grew up to 45 °C to 0.51 mol L-1 of NaCl. Bacteria which produced more auxin in the medium with l-tryptophan and two Rhizobium and one Bradyrhizobium were phosphate solubilizers. All bacteria nodulated and ESA 90 (Rhizobium sp.) plus ESA 96 (Paraburkholderia sp.) were more efficient symbiotically. Diverse and efficient rhizobia inhabit the soils of Caatinga dry forests, with the bacterial differentiation by the sampling sites.
Descritores: Rhizobium/fisiologia
Simbiose
Bradyrhizobium/fisiologia
Erythrina/microbiologia
-Fenótipo
Filogenia
Rhizobium/isolamento & purificação
Rhizobium/genética
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Cloreto de Sódio/metabolismo
Florestas
Bradyrhizobium/isolamento & purificação
Bradyrhizobium/genética
Erythrina/fisiologia
Responsável: BR1.1 - BIREME


  3 / 43 LILACS  
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Id: biblio-951793
Autor: Wu, Xiukun; Zhang, Gaosen; Zhang, Wei; Liu, Guangxiu; Chen, Tuo; Wang, Yun; Long, Haozhi; Tai, Xisheng; Zhang, Baogui; Li, Zhongqin.
Título: Variations in culturable bacterial communities and biochemical properties in the foreland of the retreating Tianshan No. 1 glacier
Fonte: Braz. j. microbiol;49(3):443-451, July-Sept. 2018. tab, graf.
Idioma: en.
Projeto: Natural Science Foundation of China; . National Basic Research Program (973) of China; . China Postdoctoral Science Fund.
Resumo: Abstract As a glacier retreats, barren areas are exposed, and these barren areas are ideal sites to study microbial succession. In this study, we characterized the soil culturable bacterial communities and biochemical parameters of early successional soils from a receding glacier in the Tianshan Mountains. The total number of culturable bacteria ranged from 2.19 × 105 to 1.30 × 106 CFU g-1 dw and from 9.33 × 105 to 2.53 × 106 CFU g-1 dw at 4 °C and 25 °C, respectively. The number of culturable bacteria in the soil increased at 25 °C but decreased at 4 °C along the chronosequence. The total organic carbon, total nitrogen content, and enzymatic activity were relatively low in the glacier foreland. The number of culturable bacteria isolated at 25 °C was significantly positively correlated with the TOC and TN as well as the soil urease, protease, polyphenoloxidase, sucrase, catalase, and dehydrogenase activities. We obtained 358 isolates from the glacier foreland soils that clustered into 35 groups using amplified ribosomal DNA restriction analysis. These groups are affiliated with 20 genera that belong to six taxa, namely, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Bacteroides, and Deinococcus-Thermus, with a predominance of members of Actinobacteria and Proteobacteria in all of the samples. A redundancy analysis showed that the bacterial succession was divided into three periods, an early stage (10a), a middle stage (25-74a), and a late stage (100-130a), with the total number of culturable bacteria mainly being affected by the soil enzymatic activity, suggesting that the microbial succession correlated with the soil age along the foreland.
Descritores: Bactérias/isolamento & purificação
Camada de Gelo/microbiologia
Camada de Gelo/química
-Filogenia
Solo/química
Microbiologia do Solo
Bactérias/classificação
Bactérias/crescimento & desenvolvimento
Bactérias/genética
DNA Bacteriano/genética
DNA Ribossômico/genética
RNA Ribossômico 16S/genética
China
Análise de Sequência de DNA
Nitrogênio/análise
Nitrogênio/metabolismo
Responsável: BR1.1 - BIREME


  4 / 43 LILACS  
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Id: biblio-839352
Autor: Dalitz, Camila de Araújo; Porsani, Mariana Vieira; Figel, Izabel Cristina; Pimentel, Ida C; Dalzoto, Patrícia R.
Título: Potential for biocontrol of melanized fungi by actinobacteria isolated from intertidal region of Ilha Do Mel, Paraná, Brazil
Fonte: Braz. j. microbiol;48(1):32-36, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. Identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16S region of ribosomal DNA (rDNA), which is conserved and present in all bacteria, is an important method of identification. Melanized fungi are free-living organisms, which can also be pathogens of clinical importance. This work aimed to evaluate growth inhibition of melanized fungi by actinobacteria and to identify the latter to the species level. In this study, antimicrobial activity of 13 actinobacterial isolates from the genus Streptomyces was evaluated against seven melanized fungi of the genera Exophiala, Cladosporium, and Rhinocladiella. In all tests, all actinobacterial isolates showed inhibitory activity against all isolates of melanized fungi, and only one actinobacterial isolate had less efficient inhibitory activity. The 16S rDNA region of five previously unidentified actinobacterial isolates from Ilha do Mel, Paraná, Brazil, was sequenced; four of the isolates were identified as Streptomyces globisporus subsp. globisporus, and one isolate was identified as Streptomyces aureus. This work highlights the potential of actinobacteria with antifungal activity and their role in the pursuit of novel antimicrobial substances.
Descritores: Actinobacteria/fisiologia
Fungos/fisiologia
Antibiose
-Filogenia
Streptomyces/classificação
Streptomyces/genética
Brasil
RNA Ribossômico 16S/genética
Actinobacteria/isolamento & purificação
Actinobacteria/classificação
Actinobacteria/genética
Responsável: BR1.1 - BIREME


  5 / 43 LILACS  
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Id: biblio-839350
Autor: Cardoso, Aline Assis; Andraus, Michel de Paula; Borba, Tereza Cristina de Oliveira; Martin-Didonet, Claudia Cristina Garcia; Ferreira, Enderson Petrônio de Brito.
Título: Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean
Fonte: Braz. j. microbiol;48(1):43-50, Jan.-Mar. 2017. tab, graf.
Idioma: en.
Resumo: Abstract This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversity and the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of common bean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The isolates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at different temperatures (28 °C, 33 °C, 38 °C, 43 °C and 48 °C). Genotypic characterization was performed based on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbiotic efficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among 98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to temperature, while 24 isolates and Rhizobium tropici CIAT899 were able to use all of the carbon sources studied. Clustering analysis based on the ability to use carbon sources and on the tolerance to salinity and temperature grouped 49 isolates, R. tropici CIAT899 and R. tropici H12 with a similarity level of 76%. Based on genotypic characterization, 65% of the isolates showed an approximately 66% similarity with R. tropici CIAT899 and R. tropici H12. About 20% of the isolates showed symbiotic efficiency similar to or better than the best Rhizobium reference strain (R. tropici CIAT899). Phylogenetic analysis of the 16S rRNA revealed that two efficient isolates (ALSG5A1 and JPrG6A8) belong to the group of strains used as commercial inoculant for common bean in Brazil and must be assayed in field experiments.
Descritores: Rhizobium/fisiologia
Simbiose
Phaseolus/genética
Phaseolus/microbiologia
Nódulos Radiculares de Plantas/microbiologia
Genótipo
-Filogenia
Rhizobium/isolamento & purificação
Rhizobium/classificação
Adaptação Biológica
Carbono/metabolismo
RNA Ribossômico 16S/genética
Phaseolus/classificação
Meio Ambiente
Tolerância a Sal
Responsável: BR1.1 - BIREME


  6 / 43 LILACS  
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Id: biblio-974306
Autor: Dealtry, Simone; Ghizelini, Angela Michelato; Mendonça-Hagler, Leda C. S; Chaloub, Ricardo Moreira; Reinert, Fernanda; Campos, Tácio M. P. de; Gomes, Newton C. M; Smalla, Kornelia.
Título: Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana
Fonte: Braz. j. microbiol;49(4):757-769, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Deutsche Forschungsgemeinschaft; . FAPERJ-Brazil.
Resumo: ABSTRACT Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while the bacterial community was rather stable, pronounced shifts were observed in the abundance of bacteria carrying plasmids. A PCR-Southern blot hybridization analysis indicated an increase in the abundance of IncP-9 catabolic plasmids. Denaturing gradient gel electrophoresis of naphthalene dioxygenase (ndo) genes amplified from cDNA (RNA) indicated the dominance of a specific ndo gene in the inoculated petroleum amendment treatment. The petroleum hydrocarbon degrading consortium characterization indicated the prevalence of bacteria assigned to Pseudomonas spp., Comamonas spp. and Ochrobactrum spp. IncP-9 plasmids were detected for the first time in Comamonas sp. and Ochrobactrum spp., which is a novelty of this study.
Descritores: Bactérias/isolamento & purificação
Bactérias/metabolismo
Avicennia/microbiologia
Hidrocarbonetos/metabolismo
-Plasmídeos/genética
Plasmídeos/metabolismo
Poluentes do Solo/análise
Poluentes do Solo/metabolismo
Bactérias/classificação
Bactérias/genética
Biodegradação Ambiental
DNA Bacteriano/genética
Petróleo/análise
RNA Ribossômico 16S/genética
Poluição por Petróleo/análise
Avicennia/metabolismo
Rizosfera
Responsável: BR1.1 - BIREME


  7 / 43 LILACS  
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Id: biblio-974305
Autor: Leite, Jakson; Passos, Samuel Ribeiro; Simões-Araújo, Jean Luiz; Rumjanek, Norma Gouvêa; Xavier, Gustavo Ribeiro; Zilli, Jerri Édson.
Título: Genomic identification and characterization of the elite strains Bradyrhizobium yuanmingense BR 3267 and Bradyrhizobium pachyrhizi BR 3262 recommended for cowpea inoculation in Brazil
Fonte: Braz. j. microbiol;49(4):703-713, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Resumo: ABSTRACT The leguminous inoculation with nodule-inducing bacteria that perform biological nitrogen fixation is a good example of an "eco-friendly agricultural practice". Bradyrhizobium strains BR 3267 and BR 3262 are recommended for cowpea (Vigna unguiculata) inoculation in Brazil and showed remarkable responses; nevertheless neither strain was characterized at species level, which is our goal in the present work using a polyphasic approach. The strains presented the typical phenotype of Bradyrhizobium with a slow growth and a white colony on yeast extract-mannitol medium. Strain BR 3267 was more versatile in its use of carbon sources compared to BR 3262. The fatty acid composition of BR 3267 was similar to the type strain of Bradyrhizobium yuanmingense; while BR 3262 was similar to Bradyrhizobium elkanii and Bradyrhizobium pachyrhizi. Phylogenetic analyses based on 16S rRNA and three housekeeping genes placed both strains within the genus Bradyrhizobium: strain BR 3267 was closest to B. yuanmingense and BR 3262 to B. pachyrhizi. Genome average nucleotide identity and DNA-DNA reassociation confirmed the genomic identification of B. yuanmingense BR 3267 and B. pachyrhizi BR 3262. The nodC and nifH gene analyses showed that strains BR 3267 and BR 3262 hold divergent symbiotic genes. In summary, the results indicate that cowpea can establish effective symbiosis with divergent bradyrhizobia isolated from Brazilian soils.
Descritores: Bradyrhizobium/isolamento & purificação
Bradyrhizobium/genética
Inoculantes Agrícolas/isolamento & purificação
Inoculantes Agrícolas/genética
Vigna/microbiologia
-Filogenia
Simbiose
Brasil
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Genoma Bacteriano
Evolução Molecular
Bradyrhizobium/classificação
Bradyrhizobium/fisiologia
Genômica
Nódulos Radiculares de Plantas/microbiologia
Inoculantes Agrícolas/classificação
Inoculantes Agrícolas/fisiologia
Vigna/fisiologia
Responsável: BR1.1 - BIREME


  8 / 43 LILACS  
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Id: biblio-974298
Autor: Vásquez-Ponce, Felipe; Higuera-Llantén, Sebastián; Pavlov, María S; Marshall, Sergio H; Olivares-Pacheco, Jorge.
Título: Phylogenetic MLSA and phenotypic analysis identification of three probable novel Pseudomonas species isolated on King George Island, South Shetland, Antarctica
Fonte: Braz. j. microbiol;49(4):695-702, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: Pontificia Universidad Católica de Valparaíso; . Programa de Investigación Asociativa.
Resumo: ABSTRACT Antarctica harbors a great diversity of microorganisms, including bacteria, archaea, microalgae and yeasts. The Pseudomonas genus is one of the most diverse and successful bacterial groups described to date, but only eight species isolated from Antarctica have been characterized. Here, we present three potentially novel species isolated on King George Island. The most abundant isolates from four different environments, were genotypically and phenotypically characterized. Multilocus sequence analysis and 16S rRNA gene analysis of a sequence concatenate for six genes (16S, aroE, glnS, gyrB, ileS and rpoD), determined one of the isolates to be a new Pseudomonas mandelii strain, while the other three are good candidates for new Pseudomonas species. Additionally, genotype analyses showed the three candidates to be part of a new subgroup within the Pseudomonas fluorescens complex, together with the Antarctic species Pseudomonas antarctica and Pseudomonas extremaustralis. We propose terming this new subgroup P. antarctica. Likewise, phenotypic analyses using API 20 NE and BIOLOG® corroborated the genotyping results, confirming that all presented isolates form part of the P. fluorescens complex. Pseudomonas genus research on the Antarctic continent is in its infancy. To understand these microorganisms' role in this extreme environment, the characterization and description of new species is vital.
Descritores: Filogenia
Pseudomonas/isolamento & purificação
Pseudomonas/classificação
-Fenótipo
Pseudomonas/genética
Microbiologia do Solo
DNA Bacteriano/genética
DNA Ribossômico/genética
RNA Ribossômico 16S/genética
Tipagem de Sequências Multilocus
Ilhas
Genótipo
Regiões Antárticas
Responsável: BR1.1 - BIREME


  9 / 43 LILACS  
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Id: biblio-974296
Autor: Goes, Kelly Campos Guerra Pinheiro de; Lovato, Gisele Milani; Andrade, Diva S.
Título: Composition of bacterial community in enrichment cultures of shale by-products from Irati Formation, Brazil
Fonte: Braz. j. microbiol;49(4):742-748, Oct.-Dec. 2018. graf.
Idioma: en.
Projeto: Fundação Araucária.
Resumo: ABSTRACT We examined microbial communities from enriched fine and retorted shale particles using sequencing of V4 variable region of 16S rRNA. High number of microbial genera was found in both enriched shale by-products that were dominate by Actinobacteria, Firmicutes and Proteobacteria, showing differences due to microbial colonization after the pyrolysis process.
Descritores: Bactérias/isolamento & purificação
Resíduos/análise
Sedimentos Geológicos/microbiologia
Microbiota
-Filogenia
Bactérias/classificação
Bactérias/genética
Brasil
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Sedimentos Geológicos/química
Biodiversidade
Responsável: BR1.1 - BIREME


  10 / 43 LILACS  
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Vasconcellos, Silvio Arruda
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Id: biblio-974290
Autor: Karcher, Daniel; Grenfell, Rafaella C; Moreno, Andrea Micke; Moreno, Luisa Zanolli; Vasconcellos, Silvio Arruda; Heinemann, Marcos B; Almeida Junior, Joao N. de; Juliano, Luiz; Juliano, Maria A.
Título: Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry
Fonte: Braz. j. microbiol;49(4):900-908, Oct.-Dec. 2018. tab, graf.
Idioma: en.
Projeto: FAPESP; . CNPq.
Resumo: ABSTRACT Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection of well-identified Brazilian isolates kept in the Bacterial Zoonosis Laboratory at the Veterinary Preventive Medicine and Animal Health Department at Sao Paulo University. In addition, L. interrogans and L. kirschneri were differentiated using an in-depth mass spectrometry analysis with ClinProTools™ software. In conclusion, our in-house reference spectra database has the necessary accuracy to differentiate pathogenic and non-pathogenic species and to distinguish L. interrogans and L. kirschneri.
Descritores: Técnicas de Tipagem Bacteriana/métodos
Espectrometria de Massas em Tandem/métodos
Leptospira/isolamento & purificação
Leptospirose/microbiologia
-Brasil
DNA Bacteriano/genética
RNA Ribossômico 16S/genética
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
Leptospira/classificação
Leptospira/genética
Leptospira/química
Limites: Seres Humanos
Tipo de Publ: Estudos de Avaliação
Responsável: BR1.1 - BIREME



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