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Pesquisa : D13.444.735.828 [Categoria DeCS]
Referências encontradas : 447 [refinar]
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Id: biblio-1101102
Autor: Farias, Lucas de Pádua Gomes de; Pereira, Helena Alves Costa; Anastacio, Eduardo Pinheiro Zarattini; Minenelli, Fernanda Formagio; Teles, Gustavo Borges da Silva.
Título: The halo sign as a chest computed tomography finding of COVID-19 / O sinal do halo como apresentação tomográfica pulmonar na COVID-19
Fonte: Einstein (Säo Paulo);18:eAI5742, 2020. graf.
Idioma: en.
Descritores: Pneumonia Viral/diagnóstico por imagem
-RNA Viral/análise
Tomografia Computadorizada por Raios X
Infecções por Coronavirus/diagnóstico por imagem
Pandemias
Reação em Cadeia da Polimerase em Tempo Real
Betacoronavirus/isolamento & purificação
Betacoronavirus/genética
SARS-CoV-2
COVID-19
Pulmão/diagnóstico por imagem
Limites: Humanos
Masculino
Adulto
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


  2 / 447 LILACS  
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Id: biblio-1045801
Autor: Zhang, Q; Shou, C; Liu, X; Yu, H; Yu, J.
Título: Epstein-Barr virus associated lymphoepithelioma-like carcinoma at the lesser curvature of the upper gastric body: a Case Report / El virus de Epstein-Barr asociado al carcinoma de tipo linfoepitelioma en la curvatura menor de la parte superior del cuerpo gástrico: Reporte de Caso
Fonte: West Indian med. j;63(1):112-114, Jan. 2014. ilus.
Idioma: en.
Resumo: Lymphoepithelioma-like gastric carcinoma (LELGC) is a rare neoplasm of the stomach with dense lymphocytic infiltration. More than 80% of LELGCs are positive for the Epstein-Barr virus (EBV). Here, we report a 64-year old Chinese man with swallowing discomfort while eating food. Endoscopy and computed tomography both showed a submucosal lesion at the lesser curvature of the upper gastric body. The first diagnostic impression was a gastrointestinal stromal tumour. Subsequently, the patient received a wedge resection of the stomach. On histopathological examination, the tumour was found to consist of small nests of neoplastic cells within dense lymphocytic infiltration. Additionally, most of the neoplastic cells were positive for cytokeratin and Epstein-Barr virus-encoded RNA (EBER). Subsequently, the diagnosis of LELGC was made. We believe that physicians should be aware of the diagnosis of submucosal gastric lesions, particularly in older male patients.

El carcinoma gástrico de tipo linfoepitelioma (CGLE) es una neoplasia rara del estómago con una infiltración linfocítica densa. Más del 80% de los CGLEs son positivos al virus de Epstein-Barr (EBV). Aquí reportamos el caso de un paciente chino de 64 años, que sentía malestar al efectuar la deglución de alimentos. Tanto la endoscopia como la tomografía computarizada mostraron una lesión submucosa en la curvatura menor de la parte superior del cuerpo gástrico. La primera impresión diagnóstica fue de un tumor del estroma gastrointestinal Posteriormente, al paciente se le hizo una resección en cuña del estómago. En el examen histopatológico, se halló que el tumor consistía de pequeños nidos de células neoplásicas dentro de una infiltración linfocítica densa. Además, la mayoría de las células neoplásicas eran positivas a la citoqueratina y al ARN codificado por el virus de Epstein-Barr (EBER). Posteriormente, se realizó el diagnóstico de CGLE. Creemos que los médicos deben tomar conciencia del diagnóstico de las lesiones submucosas gástricas, especialmente en los pacientes mayores hombres.
Descritores: Neoplasias Gástricas/diagnóstico
Carcinoma/diagnóstico
Herpesvirus Humano 4/genética
-Neoplasias Gástricas/virologia
RNA Viral/análise
Carcinoma/virologia
Linfócitos/patologia
Limites: Humanos
Masculino
Pessoa de Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


  3 / 447 LILACS  
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Id: biblio-950718
Autor: Acharya, Arpan; Vaniawala, Salil; Shah, Parth; Parekh, Harsh; Misra, Rabindra Nath; Wani, Minal; Mukhopadhyaya, Pratap N.
Título: A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting
Fonte: Biol. Res;47:1-8, 2014. graf, tab.
Idioma: en.
Resumo: BACKGROUND: Human Immunodeficiency Virus Type 1 (HIV-1) viral load testing at regular intervals is an integral component of disease management in Acquired Immunodeficiency Syndrome (AIDS) patients. The need in countries like India is therefore an assay that is not only economical but efficient and highly specific for HIV-1 sub type C virus. This study reports a SYBR Green-based HIV-1 real time PCR assay for viral load testing and is designed for enhanced specificity towards HIV-1 sub type C viruses prevalent in India. RESULTS: Linear regression of the observed and reference concentration of standards used in this study generated a correlation coefficient of 0.998 (p<0.001). Lower limit of detection of the test protocol was 50 copies/ml of plasma. The assay demonstrated 100% specificity when tested with negative control sera. The Spearman coefficient of the reported assay with an US-FDA approved, Taqman probe-based commercial kit was found to be 0.997. No significant difference in viral load was detected when the SYBR Green based assay was used to test infected plasma stored at -20°C and room temperature for 7 days respectively (Wilcoxon signed rank test, p=0.105). In a comparative study on 90 pretested HIV-1 positive samples with viral loads ranging from 5,000 - 25,000 HIV-1 RNA copies/ml and between two commercial assays it was found that the later failed to amplify in 13.33% and 10% samples respectively while in 7.77% and 4.44% samples the copy number values were reduced by >0.5 log value, a figure that is considered clinically significant by physicians. CONCLUSION: The HIV-1 viral load assay reported in this study was found to be robust, reliable, economical and effective in resource limited settings such as those existing in India. PCR probes specially designed from HIV-1 Subtype C-specific nucleotide sequences originating from India imparted specificity towards such isolates and demonstrated superior results when compared to two similar commercial assays widely used in India.
Descritores: RNA Viral/sangue
Infecções por HIV/diagnóstico
HIV-1/isolamento & purificação
Carga Viral/métodos
-Compostos Orgânicos
Kit de Reagentes para Diagnóstico/economia
Sequência de Bases/genética
Genes gag/genética
Modelos Lineares
Sensibilidade e Especificidade
HIV-1/classificação
Estatísticas não Paramétricas
Gerenciamento Clínico
Limite de Detecção
Reação em Cadeia da Polimerase em Tempo Real
Invenções
Índia
Limites: Humanos
Tipo de Publ: Estudo de Validação
Responsável: CL1.1 - Biblioteca Central


  4 / 447 LILACS  
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Id: lil-776529
Autor: Oliveira, Danilo Bretas de; Machado, Guilherme; Almeida, Gabriel Magno de Freitas; Ferreira, Paulo César Peregrino; Bonjardim, Cláudio Antônio; Trindade, Giliane de Souza; Abrahão, Jônatas Santos; Kroon, Erna Geessien.
Título: Infection of the central nervous system with dengue virus 3 genotype I causing neurological manifestations in Brazil
Fonte: Rev. Soc. Bras. Med. Trop;49(1):125-129, Jan.-Feb. 2016. graf.
Idioma: en.
Resumo: Abstract: A case of dengue virus 3 (DENV-3) genotype I infection with neurological manifestations occurred in Belo Horizonte, Minas Gerais in October 2012. The serotype was detected by PCR, and the genotype was assessed by sequencing and phylogenetic analysis of the C-prM region. The virus causing neurological manifestations clustered with other sequences of DENV-3 genotype I. Because neurological manifestations of DENV are possibly misdiagnosed in Brazil, this study serves as an alert of the importance of DENV diagnoses in CNS infections.
Descritores: Viroses do Sistema Nervoso Central/virologia
Dengue/virologia
Vírus da Dengue/genética
-Filogenia
RNA Viral/genética
Viroses do Sistema Nervoso Central/complicações
Dengue/complicações
Genótipo
Limites: Humanos
Feminino
Adulto Jovem
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


  5 / 447 LILACS  
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Id: lil-785787
Autor: Souza, Ariani Impieri; Ferreira, Ana Laura Carneiro Gomes; Arraes, Matheus Alencar; Moura, Bruno Marcelo; Braga, Maria Cynthia.
Título: Dengue as a cause of fever during pregnancy: a report of two cases
Fonte: Rev. Soc. Bras. Med. Trop;49(3):380-382tab.
Idioma: en.
Resumo: Abstract: Dengue infection has not been routinely investigated among pregnant women and parturients with acute febrile syndrome in endemic settings. Here, we report two cases of dengue fever detected at the time of delivery in parturients enrolled in a cohort prospective study conducted in a hospital in Recife, Brazil. The parturients reported fever onset within seven days prior to delivery, and dengue infection was confirmed upon detection of viral ribonucleic acid (RNA) by using the reverse transcriptase-polymerase chain reaction. Dengue infection should be considered as a diagnostic possibility in cases of fever during pregnancy and labor, especially in endemic areas.
Descritores: Complicações Infecciosas na Gravidez/diagnóstico
Dengue/diagnóstico
-Complicações Infecciosas na Gravidez/virologia
RNA Viral
Estudos Prospectivos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limites: Humanos
Feminino
Gravidez
Adolescente
Adulto Jovem
Tipo de Publ: Relatos de Casos
Responsável: BR1.1 - BIREME


  6 / 447 LILACS  
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Id: lil-785796
Autor: Romeiro, Marilia Farignoli; Souza, William Marciel de; Tolardo, Aline Lavado; Vieira, Luiz Carlos; Colombo, Tatiana Elias; Aquino, Victor Hugo; Nogueira, Maurício Lacerda; Figueiredo, Luiz Tadeu Moraes.
Título: Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil
Fonte: Rev. Soc. Bras. Med. Trop;49(3):279-285tab, graf.
Idioma: en.
Projeto: Fundação de Amparo à Pesquisa do Estado de São Paulo; . CNPq.
Resumo: Abstract: INTRODUCTION: The genus Flavivirus includes several pathogenic species that cause severe illness in humans. Therefore, a rapid and accurate molecular method for diagnosis and surveillance of these viruses would be of great importance. Here, we evaluate and optimize a quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) method for the diagnosis of the Flavivirus genus. METHODS: We evaluated different commercial kits that use the SYBR Green system for real-time RT-PCR with a primer set that amplifies a fragment of the NS5 flavivirus gene. The specificity and sensitivity of the assay were tested using twelve flaviviruses and ribonucleic acid (RNA) transcribed from the yellow fever virus. Additionally, this assay was evaluated using the sera of 410 patients from different regions of Brazil with acute febrile illness and a negative diagnosis for the dengue virus. RESULTS: The real-time RT-PCR amplified all flaviviruses tested at a melting temperature of 79.92 to 83.49°C. A detection limit of 100 copies per ml was determined for this assay. Surprisingly, we detected dengue virus in 4.1% (17/410) of samples from patients with febrile illness and a supposedly negative dengue infection diagnosis. The viral load in patients ranged from 2.1×107to 3.4×103copies per ml. CONCLUSIONS: The real-time RT-PCR method may be very useful for preliminary diagnoses in screenings, outbreaks, and other surveillance studies. Moreover, this assay can be easily applied to monitor viral activity and to measure viral load in pathogenesis studies.
Descritores: Infecções por Flavivirus/diagnóstico
Flavivirus/genética
-Compostos Orgânicos
Kit de Reagentes para Diagnóstico
Brasil
RNA Viral/genética
Sensibilidade e Especificidade
Infecções por Flavivirus/virologia
Primers do DNA
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Flavivirus/isolamento & purificação
Flavivirus/classificação
Corantes Fluorescentes
Limites: Humanos
Tipo de Publ: Estudo de Avaliação
Responsável: BR1.1 - BIREME


  7 / 447 LILACS  
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Id: biblio-896994
Autor: Esposito, Danillo Lucas Alves; Fonseca, Benedito Antonio Lopes da.
Título: Sensitivity and detection of chikungunya viral genetic material using several PCR-based approaches
Fonte: Rev. Soc. Bras. Med. Trop;50(4):465-469, July-Aug. 2017. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION: Chikungunya fever is a condition resulting from infection by chikungunya virus (CHIKV), an Aedes sp.-transmitted virus. This disease has been diagnosed in thousands of cases in the Americas, particularly in Brazil, in recent years, and there is an ongoing epidemic of chikungunya fever in Brazil that began in 2014. Clinical diagnosis is difficult; only a few cases have been confirmed by laboratory tests due to the low number of specific, efficient tests available for virus or antibody detection. Here, we aimed to evaluate different polymerase chain reaction (PCR) approaches for detection of CHIKV genetic material. METHODS: Specific primers and probes within the viral capsid gene region were designed for this work. To evaluate the analytic sensitivity of detection, human sera were spiked with serial dilutions of the viral stock. Several PCR protocols were performed to investigate the sensitivity of CHIKV RNA detection in serum dilutions ranging from 106 to 1 PFU equivalents. RESULTS: The technique showing the greatest sensitivity was a real-time PCR assay using specific probes that could detect the genetic material of the virus at all dilutions, followed by conventional PCR. Digital PCR showed low sensitivity and was much more expensive than other technologies. Digital PCR should be used for specific purposes other than clinical diagnosis. CONCLUSIONS: Although quantitative PCR using probes was more expensive than the use of intercalating dyes or conventional PCR, it had the highest sensitivity out of all tested PCR approaches.
Descritores: RNA Viral/análise
Vírus Chikungunya/genética
Primers do DNA/genética
Febre de Chikungunya/diagnóstico
-Sensibilidade e Especificidade
Reação em Cadeia da Polimerase em Tempo Real
Limites: Humanos
Responsável: BR1.1 - BIREME


  8 / 447 LILACS  
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Martelli, Celina Maria Turchi
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Id: biblio-1041413
Autor: Tassara, Marianna Peres; Guilarde, Adriana Oliveira; Rocha, Benigno Alberto Moraes da; Féres, Valéria Christina de Rezende; Martelli, Celina Maria Turchi.
Título: Neurological manifestations of dengue in Central Brazil
Fonte: Rev. Soc. Bras. Med. Trop;50(3):379-382, May-June 2017. tab.
Idioma: en.
Projeto: CNPq.
Resumo: Abstract INTRODUCTION: The incidence of dengue has increased throughout the 2000s with a consequent global increase in atypical clinical forms. METHODS: This study reports a series of cases of neurological dengue out of 498 confirmed cases of laboratory dengue in Goiânia, Brazil. Cases were confirmed based on viral RNA detection via polymerase chain reaction or IgM antibody capture. RESULTS: Neurological symptoms occurred in 5.6% of cases, including paresthesia (3.8%), encephalitis (2%), encephalopathy (1%), seizure (0.8%), meningoencephalitis (0.4%), and paresis (0.4%). DENV-3 was the predominant circulating serotype (93%). CONCLUSIONS: We reported dengue cases with neurological manifestations in endemic area.
Descritores: Parestesia/virologia
Ensaio de Imunoadsorção Enzimática
Encefalite Viral/virologia
Dengue/complicações
Dengue/epidemiologia
Meningoencefalite/virologia
-Parestesia/epidemiologia
Brasil/epidemiologia
RNA Viral/genética
Reação em Cadeia da Polimerase
Encefalite Viral/epidemiologia
Vírus da Dengue/genética
Vírus da Dengue/imunologia
Meningoencefalite/epidemiologia
Pessoa de Meia-Idade
Anticorpos Antivirais/sangue
Limites: Humanos
Masculino
Feminino
Idoso
Responsável: BR1.1 - BIREME


  9 / 447 LILACS  
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Campos, Norberto Camilo
Brigido, Luís Fernando de Macedo
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Id: biblio-1041396
Autor: Matsuda, Elaine Monteiro; Colpas, Daniela Rodrigues; Campos, Norberto Camilo; Coelho, Luana Portes Ozorio; Carmo, Andreia Moreira dos Santos; Brígido, Luís Fernando de Macedo.
Título: Undiagnosed acute HIV infection identified through RNA testing of pooled serum samples obtained during a dengue outbreak in São Paulo, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;50(1):110-112, Jan.-Feb. 2017. tab.
Idioma: en.
Projeto: FAPESP.
Resumo: Abstract INTRODUCTION: Improving HIV diagnostics and treatment is necessary to end the AIDS epidemic. Pooled plasma can be used to identify patients with acute HIV disease, even before serological tests. During dengue outbreaks, patients having symptoms common to other acute viral diseases might seek medical care. METHODS: We evaluated HIV RNA in pooled seronegative dengue samples. RESULTS: After excluding individuals with a known HIV diagnosis, an HIV-1 prevalence of 0.73% [95% confidence interval (CI) 0.23-1.76; 4/546 samples] was found. CONCLUSIONS: Promoting strategies to diagnose these individuals and provide them with medical treatment might be instrumental for controlling the HIV epidemic.
Descritores: RNA Viral/sangue
Infecções por HIV/diagnóstico
Surtos de Doenças
HIV-1/genética
Dengue/epidemiologia
-Brasil/epidemiologia
Infecções por HIV/epidemiologia
Doença Aguda
Prevalência
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adulto
Responsável: BR1.1 - BIREME


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Id: biblio-1041479
Autor: Guimarães, Vanessa de Souza; Melo, Talita Gomes de; Ferreira, Rita de Cássia Damasceno; Almeida, Sherley Ferreira de; Martins, Luisa Caricio.
Título: Prevalence of hepatitis C virus genotypes in the State of Pará, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;51(4):508-512, July-Aug. 2018. tab, graf.
Idioma: en.
Resumo: Abstract INTRODUCTION This study reports the genotype prevalence of hepatitis C virus (HCV) in Pará, Brazil. METHODS: A retrospective cross-sectional study was conducted on 344 plasma samples sent to the Lacen-Pará for diagnostics by molecular techniques. RESULTS: HCV genotypes identified in the different regions of Pará were 1b (47.7%), 3 (23.3%), 1a (18%), and 2 (4.4%). Genotype 1 occurred in 41.6% of men and 30.8% of women in the 18-86-year-old group. CONCLUSIONS: Genotype 1 is the most predominant in Pará, which reinforces the idea of its relationship with late-diagnosed chronic infection.
Descritores: Hepacivirus/genética
Hepatite C Crônica/epidemiologia
-Brasil/epidemiologia
RNA Viral/genética
Prevalência
Estudos Transversais
Estudos Retrospectivos
Hepatite C Crônica/virologia
Genótipo
Pessoa de Meia-Idade
Limites: Humanos
Masculino
Feminino
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Adulto Jovem
Responsável: BR1.1 - BIREME



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